RLG00000029880

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004224
Physical Location & Seq
Forward (+)
50656344 .. 50660037
3694 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029880

Sequence Viewer

Length: 2118 bp
ATGCTTCTTATGAAACTCTCATTAGTCTTATTAGCAGCTGCAACGACATTGCTAGCTGATAAAACCCCCTCACCGCCTCAAGCCAAGCCCAACTGCCCAGACCGCTGTGGTAACCTCACAATTCCATATCCATTTGGCATCGGTGACGGCTGTTACATACCACTGCAGGGACAGGAAAAGCAATTCGAACTCGCTTGTAATTATTCTTCTAACCCCCCATCATTGACCTGGACGTACCCCTCCGTCAGTATTTCTGGTTTTCACCTTGCTGAGGGTGAGTTGGAAGTCATGAATGGGATAAGCAAGGACTGTTACAACAAGAGCGGTTTTAATGTAAAGAAATCCATTCCTGGGCTCGCTTTGTCTCCTCCTCTCAGCGTTTCCGGTCAAAAAAACAACTTCGTCGCCATAGGCTGCGACACTTCTGCTGCATTCCGGGCTTTTCGTGGAGAACAAAAAATCATGATGAGGTGCACGTCCGTGTGCCTCGACATTAGCACTGTGGATCTAGACTCTTGCTCTGGTGTCGGGTGTTGCCAGACCGATATCCCTGATGGACTGAATAATCTTACCGTGAGCTTGGATAGCTATTTCAATCATTTGGATACATGGGAATTTAATCCCTGCAGCTATGCCTTCGTTGTGAAAGATGGCTACTTTAACTTCAGCGGTACGAGGAGTTTCGAAAAGCTCAAGAACATGGAGCGGGTTCCAATTATCGCTAATTGGCAGATTGGAAATAAGACATGCGATGTAGCTAAAAAGAATGCCGCGGATTACGCATGCAAGGAAAATTCCGTGTGCGCCAACCAGACCAAGGGCCCATACCCTGCAGGTTACTATTGCCAATGCTCGCCAGGCTACGAAGGGAACCCATACATCGGTTGCCAAGATATTGACGAGTGTTTGGCTGAAATTAACCCATGCGGAAATGGAACATGCAAAAATTTACCTGCAAGTTACTCGTGTAAATGTAATAAAGGGTTCAAAAATGATGGTCCTTTCAAGTGCATTAAGGTTCGTCCCACGGCAAACAACACTCCTCTGAAAATTTCATTGGGTATTTGTATAGGCTTCTTTGTTCTATTAGTCGTAAGTTTTGGGTTATATCGCGAACACAAGAGAAGACTGTTCAACAAAATTAAAATAAAGTACTTTGAAGCACATGGCGGGCAATTATTACGATCACAATTGAAGCAATTGGCTGGTCAAAAAGAATCTCTTGCAGTGCAAATCTTTACTGAAGAAGAAATTAAGAAGGCAACAAAGAATTACGATGAAAATGAAAAAATTGGTGAAGGAGCCTATGGAATAGTCTACAAAGGCGTACTCGATGATAAAAGAGAGGTTGCAATAAAGATCTCCAAAATGACTGCCCTGGTCAAAAGTGATCAATTTATCAATGAGGTCATTGTACTTTCTCAAATCAGTCATAGAAATGTGGTGGGGCTGCTAGGCTGTTGTTTGGAGGCAAAAACGCCTATGCTAGTTTACGAGTATGTTGGTAATGGAACTCTTTATGATCACATTCACAAAAAGAAAGTACAACCACTTTCATTTGCACAACGATTGAAGATAGCAGCACAAACTGCGACGGCACTTTCATACTTGCATTACTCCACCACTACGCAAATTATTCATGGAGATGTCAAGGCAACGAATATACTGTTGGATGAGAAACTTACGGCCAAATTGACGGGCTTTGGAGCTTCAAGACTTGTTCCTGACCGCAAAACTCAACTTTCAACTTTAGTGCAGGGGACAATGGGATACTTGGACCCTGAATATCTTCAGTCCAGCACACTAACAGAAAAGAGTGACGTTTATAGCTTTGGAGTTGTCCTCGTGGAGCTACTGACAAGCGAGAATGCAATTCGTTTTGATAAACCCGAGGCAGAGAGAAACCTGGCCAACGTCTTTGTTTCCACAATTGAAGAAAAAGGTCTGCGCGCATTGGGTCCAATTCTTGATGATGAAATAGTCAAGGATGGAAAGTGGGAGATCATCGAAGAAGTTGCCAATCTTGCCCAAAGATGCTTGAGGGTGAAACGAGACGCAAGGCCTACCATGGAAGAAGTTGAGTGGGAGCTAGATGAAATACTGAGAGAAGCTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK cEGF

Protein Analysis

706

Amino Acids

77.98

Weight (kDa)

7.03

Isoelectric Point (pI)

30.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 32 - 77 2.8e-08 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 298 - 337 3.5e-06 Calcium-binding EGF domain
Pkinase PF00069 425 - 693 1.1e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 428 - 695 6.5e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 824, 961
AccBSI CCGCTC 2 cut(s) 324, 706
AccI GTMKAC 1 cut(s) 1317
AccII CGCG 3 cut(s) 773, 1113, 1950
AclWI GGATC 1 cut(s) 513
AcoI YGGCCR 2 cut(s) 1686, 1908
AcsI RAATTY 4 cut(s) 614, 793, 946, 1050
AcuI CTGAAG 3 cut(s) 649, 1263, 1775
AfaI GTAC 6 cut(s) 236, 673, 1154, 1329, 1416, 1545
AfiI CCNNNNNNNGG 5 cut(s) 167, 271, 351, 817, 881
AjiI CACGTC 1 cut(s) 477
AjnI CCWGG 5 cut(s) 227, 349, 856, 1377, 1905
AjuI GAANNNNNNNTTGG 4 cut(s) 1040, 1072, 1652, 1684
AleI CACNNNNGTG 1 cut(s) 479
Alw21I GWGCWC 1 cut(s) 476
Alw26I GTCTC 2 cut(s) 369, 2046
Alw44I GTGCAC 1 cut(s) 472
AlwI GGATC 1 cut(s) 513
Ama87I CYCGRG 1 cut(s) 1889
AoxI GGCC 4 cut(s) 820, 1686, 1908, 2060
ApaI GGGCCC 1 cut(s) 824
ApaLI GTGCAC 1 cut(s) 472
ApeKI GCWGC 7 cut(s) 35, 38, 414, 428, 627, 1450, 1580
ApoI RAATTY 4 cut(s) 614, 793, 946, 1050
Asp700I GAANNNNTTC 1 cut(s) 1788
AspLEI GCGC 3 cut(s) 806, 1950, 1952
AspS9I GGNCC 5 cut(s) 820, 821, 998, 1777, 1958
AsuC2I CCSGG 1 cut(s) 437
AsuHPI GGTGA 6 cut(s) 63, 155, 254, 287, 1307, 2056
AsuII TTCGAA 2 cut(s) 186, 684
AsuNHI GCTAGC 1 cut(s) 52
AvaI CYCGRG 1 cut(s) 1889
AvaII GGWCC 3 cut(s) 998, 1777, 1958
BaeGI GKGCMC 2 cut(s) 476, 824
BalI TGGCCA 1 cut(s) 1910
BanII GRGCYC 2 cut(s) 357, 824
BarI GAAGNNNNNNTAC 2 cut(s) 190, 222
BauI CACGAG 2 cut(s) 964, 1844
BbsI GAAGAC 1 cut(s) 1132
Bbv12I GWGCWC 1 cut(s) 476
BbvCI CCTCAGC 1 cut(s) 270
BbvI GCAGC 7 cut(s) 25, 47, 401, 415, 639, 1437, 1592
BccI CCATC 5 cut(s) 226, 548, 644, 989, 1982
BceAI ACGGC 4 cut(s) 163, 1044, 1611, 1701
BcgI CGANNNNNNTGC 6 cut(s) 407, 441, 945, 979, 1997, 2031
BciT130I CCWGG 5 cut(s) 229, 351, 858, 1379, 1907
BciVI GTATCC 2 cut(s) 598, 1763
BclI TGATCA 2 cut(s) 1390, 1522
BcnI CCSGG 1 cut(s) 437
BcoDI GTCTC 2 cut(s) 369, 2046
BfaI CTAG 5 cut(s) 53, 509, 1454, 1487, 2090
BfmI CTRYAG 3 cut(s) 164, 625, 831
BfuAI ACCTGC 2 cut(s) 824, 961
BfuI GTATCC 2 cut(s) 598, 1763
BglII AGATCT 1 cut(s) 1359
BisI GCNGC 8 cut(s) 36, 39, 415, 429, 628, 771, 1451, 1581
BlsI GCNGC 8 cut(s) 37, 40, 416, 430, 629, 772, 1452, 1582
BmcAI AGTACT 1 cut(s) 1154
Bme1390I CCNGG 6 cut(s) 229, 351, 437, 858, 1379, 1907
Bme18I GGWCC 3 cut(s) 998, 1777, 1958
BmeT110I CYCGRG 1 cut(s) 1889
BmgBI CACGTC 1 cut(s) 477
BmgT120I GGNCC 5 cut(s) 820, 821, 998, 1777, 1958
BmiI GGNNCC 6 cut(s) 711, 822, 872, 1303, 1779, 1959
BmrFI CCNGG 6 cut(s) 229, 351, 437, 858, 1379, 1907
BmsI GCATC 2 cut(s) 147, 2024
BmtI GCTAGC 1 cut(s) 56
BpiI GAAGAC 1 cut(s) 1132
BplI GAGNNNNNCTC 2 cut(s) 1827, 1859
Bpu10I CCTNAGC 1 cut(s) 270
Bpu14I TTCGAA 2 cut(s) 186, 684
BpuEI CTTGAG 3 cut(s) 63, 677, 2059
BpuMI CCSGG 1 cut(s) 437
BsaJI CCNNGG 7 cut(s) 350, 771, 816, 1026, 1377, 1890, 2067
BsaWI WCCGGW 1 cut(s) 383
Bsc4I CCNNNNNNNGG 5 cut(s) 167, 271, 351, 817, 881
Bse3DI GCAATG 1 cut(s) 47
BseBI CCWGG 5 cut(s) 229, 351, 858, 1379, 1907
BseDI CCNNGG 7 cut(s) 350, 771, 816, 1026, 1377, 1890, 2067
BseGI GGATG 2 cut(s) 1678, 1993
BseLI CCNNNNNNNGG 5 cut(s) 167, 271, 351, 817, 881
BseMI GCAATG 1 cut(s) 47
BseMII CTCAG 3 cut(s) 261, 388, 2093
BsePI GCGCGC 1 cut(s) 1948
BseRI GAGGAG 4 cut(s) 357, 360, 691, 1032
BseSI GKGCMC 2 cut(s) 476, 824
BseXI GCAGC 7 cut(s) 25, 47, 401, 415, 639, 1437, 1592
BsgI GTGCAG 1 cut(s) 1775
Bsh1236I CGCG 3 cut(s) 773, 1113, 1950
BshFI GGCC 4 cut(s) 822, 1688, 1910, 2062
BsiHKAI GWGCWC 1 cut(s) 476
BsiHKCI CYCGRG 1 cut(s) 1889
BsiSI CCGG 2 cut(s) 384, 436
BslFI GGGAC 3 cut(s) 183, 1008, 1774
BslI CCNNNNNNNGG 5 cut(s) 167, 271, 351, 817, 881
BsmAI GTCTC 2 cut(s) 369, 2046
BsmBI CGTCTC 1 cut(s) 2046
BsmFI GGGAC 3 cut(s) 183, 1008, 1774
BsmI GAATGC 3 cut(s) 431, 772, 1873
BsnI GGCC 4 cut(s) 822, 1688, 1910, 2062
BsoBI CYCGRG 1 cut(s) 1889
Bsp119I TTCGAA 2 cut(s) 186, 684
Bsp120I GGGCCC 1 cut(s) 820
Bsp1286I GDGCHC 3 cut(s) 357, 476, 824
Bsp143I GATC 6 cut(s) 505, 1184, 1359, 1390, 1522, 2001
Bsp19I CCATGG 1 cut(s) 2067
Bsp68I TCGCGA 1 cut(s) 1113
BspANI GGCC 4 cut(s) 822, 1688, 1910, 2062
BspCNI CTCAG 3 cut(s) 262, 387, 2094
BspFNI CGCG 3 cut(s) 773, 1113, 1950
BspHI TCATGA 2 cut(s) 288, 462
BspLI GGNNCC 6 cut(s) 711, 822, 872, 1303, 1779, 1959
BspMAI CTGCAG 3 cut(s) 168, 629, 835
BspMI ACCTGC 2 cut(s) 824, 961
BspOI GCTAGC 1 cut(s) 56
BspPI GGATC 1 cut(s) 513
BspT104I TTCGAA 2 cut(s) 186, 684
BsrBI CCGCTC 2 cut(s) 324, 706
BsrDI GCAATG 1 cut(s) 47
BssECI CCNNGG 7 cut(s) 350, 771, 816, 1026, 1377, 1890, 2067
BssHII GCGCGC 1 cut(s) 1948
BssMI GATC 6 cut(s) 505, 1184, 1359, 1390, 1522, 2001
BssSI CACGAG 2 cut(s) 964, 1844
BssT1I CCWWGG 2 cut(s) 816, 2067
Bst2BI CACGAG 2 cut(s) 964, 1844
Bst2UI CCWGG 5 cut(s) 229, 351, 858, 1379, 1907
Bst4CI ACNGT 5 cut(s) 311, 502, 574, 1131, 1668
BstAPI GCANNNNNTGC 1 cut(s) 1589
BstBI TTCGAA 2 cut(s) 186, 684
BstC8I GCNNGC 6 cut(s) 54, 357, 784, 854, 1172, 1950
BstDEI CTNAG 3 cut(s) 270, 374, 2102
BstDSI CCRYGG 3 cut(s) 771, 1026, 2067
BstEII GGTNACC 1 cut(s) 110
BstENI CCTNNNNNAGG 1 cut(s) 269
BstF5I GGATG 2 cut(s) 1678, 1993
BstFNI CGCG 3 cut(s) 773, 1113, 1950
BstHHI GCGC 3 cut(s) 806, 1950, 1952
BstKTI GATC 6 cut(s) 508, 1187, 1362, 1393, 1525, 2004
BstMAI GTCTC 2 cut(s) 369, 2046
BstMBI GATC 6 cut(s) 505, 1184, 1359, 1390, 1522, 2001
BstMWI GCNNNNNNNGC 7 cut(s) 102, 437, 585, 779, 858, 1589, 2024
BstNI CCWGG 5 cut(s) 229, 351, 858, 1379, 1907
BstNSI RCATGY 3 cut(s) 750, 786, 942
BstPI GGTNACC 1 cut(s) 110
BstSCI CCNGG 6 cut(s) 227, 349, 435, 856, 1377, 1905
BstSFI CTRYAG 3 cut(s) 164, 625, 831
BstSLI GKGCMC 2 cut(s) 476, 824
BstUI CGCG 3 cut(s) 773, 1113, 1950
BstV1I GCAGC 7 cut(s) 25, 47, 401, 415, 639, 1437, 1592
BstV2I GAAGAC 1 cut(s) 1132
BstX2I RGATCY 2 cut(s) 505, 1359
BstYI RGATCY 2 cut(s) 505, 1359
BsuI GTATCC 2 cut(s) 598, 1763
BsuRI GGCC 4 cut(s) 822, 1688, 1910, 2062
BtgI CCRYGG 3 cut(s) 771, 1026, 2067
BtgZI GCGATG 1 cut(s) 765
BtrI CACGTC 1 cut(s) 477
BtsCI GGATG 2 cut(s) 1678, 1993
BtsI GCAGTG 2 cut(s) 161, 1233
BtsIMutI CAGTG 3 cut(s) 161, 498, 1233
BtuMI TCGCGA 1 cut(s) 1113
BveI ACCTGC 2 cut(s) 824, 961
Cac8I GCNNGC 6 cut(s) 54, 357, 784, 854, 1172, 1950
CciI TCATGA 2 cut(s) 288, 462
CfoI GCGC 3 cut(s) 806, 1950, 1952
Cfr13I GGNCC 5 cut(s) 820, 821, 998, 1777, 1958
Cfr42I CCGCGG 1 cut(s) 774
CseI GACGC 1 cut(s) 2063
Csp6I GTAC 6 cut(s) 235, 672, 1153, 1328, 1415, 1544
CviQI GTAC 6 cut(s) 235, 672, 1153, 1328, 1415, 1544
DdeI CTNAG 3 cut(s) 270, 374, 2102
DpnI GATC 6 cut(s) 507, 1186, 1361, 1392, 1524, 2003
DpnII GATC 6 cut(s) 505, 1184, 1359, 1390, 1522, 2001
EaeI YGGCCR 2 cut(s) 1686, 1908
Eco130I CCWWGG 2 cut(s) 816, 2067
Eco147I AGGCCT 1 cut(s) 2062
Eco24I GRGCYC 2 cut(s) 357, 824
Eco32I GATATC 1 cut(s) 547
Eco47I GGWCC 3 cut(s) 998, 1777, 1958
Eco57I CTGAAG 3 cut(s) 649, 1263, 1775
Eco88I CYCGRG 1 cut(s) 1889
Eco91I GGTNACC 1 cut(s) 110
EcoNI CCTNNNNNAGG 1 cut(s) 269
EcoO109I RGGNCCY 1 cut(s) 820
EcoO65I GGTNACC 1 cut(s) 110
EcoRII CCWGG 5 cut(s) 227, 349, 856, 1377, 1905
EcoRV GATATC 1 cut(s) 547
EcoT14I CCWWGG 2 cut(s) 816, 2067
EcoT38I GRGCYC 2 cut(s) 357, 824
ErhI CCWWGG 2 cut(s) 816, 2067
Esp3I CGTCTC 1 cut(s) 2046
FalI AAGNNNNNCTT 2 cut(s) 1206, 1238
FaqI GGGAC 3 cut(s) 183, 1008, 1774
FauI CCCGC 2 cut(s) 699, 1163
FbaI TGATCA 2 cut(s) 1390, 1522
FblI GTMKAC 1 cut(s) 1317
Fnu4HI GCNGC 8 cut(s) 36, 39, 415, 429, 628, 771, 1451, 1581
FokI GGATG 2 cut(s) 1685, 2000
FriOI GRGCYC 2 cut(s) 357, 824
Fsp4HI GCNGC 8 cut(s) 36, 39, 415, 429, 628, 771, 1451, 1581
FspBI CTAG 5 cut(s) 53, 509, 1454, 1487, 2090
GlaI GCGC 3 cut(s) 805, 1949, 1951
GluI GCNGC 8 cut(s) 36, 39, 415, 429, 628, 771, 1451, 1581
HaeIII GGCC 4 cut(s) 822, 1688, 1910, 2062
HapII CCGG 2 cut(s) 384, 436
HgaI GACGC 1 cut(s) 2063
HhaI GCGC 3 cut(s) 806, 1950, 1952
Hin6I GCGC 3 cut(s) 804, 1948, 1950
HinP1I GCGC 3 cut(s) 804, 1948, 1950
HindIII AAGCTT 1 cut(s) 2109
HinfI GANTC 2 cut(s) 512, 1217
HpaII CCGG 2 cut(s) 384, 436
HphI GGTGA 6 cut(s) 63, 155, 254, 287, 1307, 2056
Hpy166II GTNNAC 3 cut(s) 474, 1318, 1492
Hpy188I TCNGA 1 cut(s) 1047
Hpy188III TCNNGA 8 cut(s) 289, 463, 509, 694, 1112, 1713, 1724, 1967
Hpy8I GTNNAC 3 cut(s) 474, 1318, 1492
Hpy99I CGWCG 2 cut(s) 407, 1597
HpyAV CCTTC 4 cut(s) 646, 860, 1252, 1292
HpyCH4III ACNGT 5 cut(s) 311, 502, 574, 1131, 1668
HpyCH4IV ACGT 4 cut(s) 233, 476, 1821, 1914
HpyF10VI GCNNNNNNNGC 7 cut(s) 102, 437, 585, 779, 858, 1589, 2024
HpyF3I CTNAG 3 cut(s) 270, 374, 2102
HpySE526I ACGT 4 cut(s) 233, 476, 1821, 1914
HspAI GCGC 3 cut(s) 804, 1948, 1950
Ksp22I TGATCA 2 cut(s) 1390, 1522
KspI CCGCGG 1 cut(s) 774
Kzo9I GATC 6 cut(s) 505, 1184, 1359, 1390, 1522, 2001
LmnI GCTCC 5 cut(s) 703, 1301, 1706, 1849, 2086
Lsp1109I GCAGC 7 cut(s) 25, 47, 401, 415, 639, 1437, 1592
LweI GCATC 2 cut(s) 147, 2024
MaeI CTAG 5 cut(s) 53, 509, 1454, 1487, 2090
MaeII ACGT 4 cut(s) 233, 476, 1821, 1914
MaeIII GTNAC 7 cut(s) 110, 143, 152, 311, 836, 959, 1817
MalI GATC 6 cut(s) 507, 1186, 1361, 1392, 1524, 2003
MbiI CCGCTC 2 cut(s) 324, 706
MboI GATC 6 cut(s) 505, 1184, 1359, 1390, 1522, 2001
MboII GAAGA 9 cut(s) 198, 1137, 1256, 1259, 1585, 1781, 1946, 2021, 2084
MfeI CAATTG 3 cut(s) 1190, 1199, 1929
MflI RGATCY 2 cut(s) 505, 1359
MhlI GDGCHC 3 cut(s) 357, 476, 824
MlsI TGGCCA 1 cut(s) 1910
MluNI TGGCCA 1 cut(s) 1910
MlyI GAGTC 1 cut(s) 506
MmeI TCCRAC 2 cut(s) 261, 1650
Mox20I TGGCCA 1 cut(s) 1910
MroXI GAANNNNTTC 1 cut(s) 1788
MscI TGGCCA 1 cut(s) 1910
MseI TTAA 7 cut(s) 330, 618, 660, 918, 1014, 1143, 1254
MslI CAYNNNNRTG 3 cut(s) 479, 1437, 1644
Msp20I TGGCCA 1 cut(s) 1910
MspA1I CMGCKG 4 cut(s) 38, 105, 669, 773
MspI CCGG 2 cut(s) 384, 436
MspR9I CCNGG 6 cut(s) 229, 351, 437, 858, 1379, 1907
MunI CAATTG 3 cut(s) 1190, 1199, 1929
Mva1269I GAATGC 3 cut(s) 431, 772, 1873
MvaI CCWGG 5 cut(s) 229, 351, 858, 1379, 1907
MvnI CGCG 3 cut(s) 773, 1113, 1950
MwoI GCNNNNNNNGC 7 cut(s) 102, 437, 585, 779, 858, 1589, 2024
NciI CCSGG 1 cut(s) 437
NcoI CCATGG 1 cut(s) 2067
NdeII GATC 6 cut(s) 505, 1184, 1359, 1390, 1522, 2001
NheI GCTAGC 1 cut(s) 52
NlaIV GGNNCC 6 cut(s) 711, 822, 872, 1303, 1779, 1959
NmuCI GTSAC 2 cut(s) 143, 1817
NruI TCGCGA 1 cut(s) 1113
NspI RCATGY 3 cut(s) 750, 786, 942
NspV TTCGAA 2 cut(s) 186, 684
OliI CACNNNNGTG 1 cut(s) 479
PaeI GCATGC 1 cut(s) 786
PagI TCATGA 2 cut(s) 288, 462
PauI GCGCGC 1 cut(s) 1948
PceI AGGCCT 1 cut(s) 2062
PctI GAATGC 3 cut(s) 431, 772, 1873
PdmI GAANNNNTTC 1 cut(s) 1788
PfeI GAWTC 1 cut(s) 1217
PkrI GCNGC 8 cut(s) 37, 40, 416, 430, 629, 772, 1452, 1582
PleI GAGTC 1 cut(s) 506
PpsI GAGTC 1 cut(s) 506
Psp6I CCWGG 5 cut(s) 227, 349, 856, 1377, 1905
PspEI GGTNACC 1 cut(s) 110
PspGI CCWGG 5 cut(s) 227, 349, 856, 1377, 1905
PspN4I GGNNCC 6 cut(s) 711, 822, 872, 1303, 1779, 1959
PspOMI GGGCCC 1 cut(s) 820
PspPI GGNCC 5 cut(s) 820, 821, 998, 1777, 1958
PstI CTGCAG 3 cut(s) 168, 629, 835
PsuI RGATCY 2 cut(s) 505, 1359
PteI GCGCGC 1 cut(s) 1948
PvuII CAGCTG 1 cut(s) 38
RruI TCGCGA 1 cut(s) 1113
RsaI GTAC 6 cut(s) 236, 673, 1154, 1329, 1416, 1545
RsaNI GTAC 6 cut(s) 235, 672, 1153, 1328, 1415, 1544
RseI CAYNNNNRTG 3 cut(s) 479, 1437, 1644
SacII CCGCGG 1 cut(s) 774
SaqAI TTAA 7 cut(s) 330, 618, 660, 918, 1014, 1143, 1254
SatI GCNGC 8 cut(s) 36, 39, 415, 429, 628, 771, 1451, 1581
Sau3AI GATC 6 cut(s) 505, 1184, 1359, 1390, 1522, 2001
Sau96I GGNCC 5 cut(s) 820, 821, 998, 1777, 1958
SbfI CCTGCAGG 1 cut(s) 835
ScaI AGTACT 1 cut(s) 1154
SchI GAGTC 1 cut(s) 506
ScrFI CCNGG 6 cut(s) 229, 351, 437, 858, 1379, 1907
SdaI CCTGCAGG 1 cut(s) 835
SduI GDGCHC 3 cut(s) 357, 476, 824
SfaNI GCATC 2 cut(s) 147, 2024
SfcI CTRYAG 3 cut(s) 164, 625, 831
Sfr303I CCGCGG 1 cut(s) 774
SfuI TTCGAA 2 cut(s) 186, 684
SgrBI CCGCGG 1 cut(s) 774
SinI GGWCC 3 cut(s) 998, 1777, 1958
SmiMI CAYNNNNRTG 3 cut(s) 479, 1437, 1644
SmlI CTYRAG 3 cut(s) 78, 692, 2038
SmoI CTYRAG 3 cut(s) 78, 692, 2038
SphI GCATGC 1 cut(s) 786
Sse8387I CCTGCAGG 1 cut(s) 835
SseBI AGGCCT 1 cut(s) 2062
SspMI CTAG 5 cut(s) 53, 509, 1454, 1487, 2090
StuI AGGCCT 1 cut(s) 2062
StyD4I CCNGG 6 cut(s) 227, 349, 435, 856, 1377, 1905
StyI CCWWGG 2 cut(s) 816, 2067
TaaI ACNGT 5 cut(s) 311, 502, 574, 1131, 1668
TaiI ACGT 4 cut(s) 236, 479, 1824, 1917
TaqI TCGA 5 cut(s) 186, 489, 684, 1332, 2007
TaqII GACCGA 1 cut(s) 557
TatI WGTACW 3 cut(s) 1152, 1414, 1543
TauI GCSGC 1 cut(s) 773
TfiI GAWTC 1 cut(s) 1217
Tru1I TTAA 7 cut(s) 330, 618, 660, 918, 1014, 1143, 1254
Tru9I TTAA 7 cut(s) 330, 618, 660, 918, 1014, 1143, 1254
TscAI CASTG 3 cut(s) 168, 505, 1233
TseFI GTSAC 2 cut(s) 143, 1817
TseI GCWGC 7 cut(s) 35, 38, 414, 428, 627, 1450, 1580
Tsp45I GTSAC 2 cut(s) 143, 1817
TspGWI ACGGA 3 cut(s) 232, 469, 787
TspRI CASTG 3 cut(s) 168, 505, 1233
VneI GTGCAC 1 cut(s) 472
VpaK11BI GGWCC 3 cut(s) 998, 1777, 1958
XagI CCTNNNNNAGG 1 cut(s) 269
XapI RAATTY 4 cut(s) 614, 793, 946, 1050
XbaI TCTAGA 1 cut(s) 508
XceI RCATGY 3 cut(s) 750, 786, 942
XcmI CCANNNNNNNNNTGG 1 cut(s) 225
XmiI GTMKAC 1 cut(s) 1317
XmnI GAANNNNTTC 1 cut(s) 1788
XspI CTAG 5 cut(s) 53, 509, 1454, 1487, 2090
ZrmI AGTACT 1 cut(s) 1154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.