Rroxscaffold_4G00321010

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0004224
Physical Location & Seq
Forward (+)
50049947 .. 50054543
4597 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00321010.1

Sequence Viewer

Length: 2187 bp
ATGGCCTTAGTAGTACATGGGAAAATGCTTCTTATTTTAGTCTTATTAGCAGCTGCAACGACATTGCTAGCTGATAAAACCCCCTCACCGCCTCAAGCCAAGCCCAACTGCCCAGACCGCTGTGGTAACCTCACAATTCCATATCCATTTGGCATCGGTGAAGGCTGTTACTTACCACTGAAGGGAAAGGAACAGCAATTCGAACTCGCTTGTAATTATTCTACTAAACCCCCATCATTGACCTGGCCGATGGACCCCAGCTCCGTCAGTATCTCTGGTTTTCACCTTGCTGAGGGTGAGTTGGAAGTCATGAGTGCGATAAGCAAGGATTGTTACAACAAGAGCGGTTTTAATGTAGAGGAATACTTCTCTCTCTCTTGGTATCCTCCTTTCAGCCTTTCCGGTCGAAAAAACAGCTTCGTCGCCATAGGCTGCGACACTTCTGCTGCTTTCCGGGGTTTTCGTGGCAAAAAAAAAATCATGACGGGGTGCATGTCCGTGTGCCTCGATATTAACACCGTGGATCTAAACTCTTGCACCGGTGTCGGTTGTTGCCAGACCAAGATCCCTGATGAACTGAATAATTTTACTGTGAGATTGGATAGCTATTTCAATCATTCGGATATATGGGAATTCAATCCCTGCAGCTATGCCTTCGTTGTGAAAGATGGCTACTTTAACTTCAGCGGTACGAGGAGTTTCAAAGAGCTCAAGAACATGGAGCAGGTTCCATTTATCGCTAATTGGCAGATTGGAAATGAGACATGCGATGTAGCTAAAAAGAATGCCGTGAGTTATGCATGCAAGGCAAATTCCGTGTGCGTCAACCGGACCAAGAGCCCATGGCCTGCAGGTTACTACTGCCAATGCTCACCAGGCTACGAAGGGAACCCATACATCGGTTGCCGAGATATTGAGGAGTGTTTGGCTGAAATTAACCCATGCGGGAATGGAACGTGCAAGAATATACCTGGAAGTTACTCTTGTAAATGTAAAAAAGGGTTCAAAAATGATGGTCCTTTCAAGTGCATTGCTCGTCCCACGGCAAGCAACAGTGCTCTGAAAATTTCACTGGTCGTAATTTTTGGATTATATCGCGAATACAAGAGAAGGCAGTTCAACGAAATGAAAAAAAAGTATTTTGAAGCAAACGGCGGGCCAAAATTACAACTACAATTGAAGCAATTGGCTAGTCAAAAAGAACCTCTTGCAGTGCAAATCTTTACTGAAGAAGAACTTAAGAAGGCAACAAAGAATTACGATGAAAATGAAAAACTTGGAGAAGGAGGCTATGGAATAGTCTACAAAGGCGTACTCGATAAAAGAGTGGTTGCAATAAAGATATCCAAAATGAATGCCCAGGTTGAAAGTGATCAATTTATCAATGAGGTCATTGTTCTTTCTCAAATCAGTCATAGAAATGTGGTGAGGCTGCTAGGCTGTTGTTTGGAGACAAAAACGCCTATGCTAGTTTACGAGTATGTTGGTAGTGGAACTCTTTATGACCACATTCATAAAAAGAAAGGACAACTACTTTCATTTGCACAACGATTGAAGATAGCAGCAGAAACTGCGACAGCACTTTCATACTTACATCACTCCACCACCACGCAAATTGTTCATCGAGATGTCAAGGCAACGAATATACTGTTGGATGAGAAACTTATGGCCAAAGTGTCGGACTTCGGAGCTTCAAAACTTATTCCTGACGACAAAACTCAACTTTCAACTCTAGTGCAGGGGACATTGGGATACTTGGACCCTGAATATCTTCAGTCCAACACTCTGACAGAAAAGAGCCTGCGCGCATTGGGTCCAATTCTTGATGATGAAATAGTCAAGGATGGAAACTGGGTGATCATCGAAAAAGTTGCCAATCTTGCCCAAAGATGCTTAAGTGTGAAAGGAGACGAAAGGCCTACCATGAAAGAAATAGAGAGGGAGCTAGATGAAATACGGAAAACTTCCGCAAAGCAACCAGATGGGAAGAAGCTTTATGCCTCCTCTTCCAAAGAGACGGACCCATCTCTTGACTCACATTCAGACGCTTACAATGTGGAGATTAGTGATGAAGGCGATGGTGGTTCTACTGGCATTATTAGCAGTACAGAGTATGACGCCAGCATGCAAAATCAAGCCCAAAAGTCTTCCAGAGTTATGGTCGATAAAAATTCTTCGGCCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK cEGF

Protein Analysis

728

Amino Acids

80.63

Weight (kDa)

7.98

Isoelectric Point (pI)

31.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 37 - 96 1.8e-08 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 304 - 343 2.1e-07 Calcium-binding EGF domain
Pkinase PF00069 420 - 599 6.7e-38 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 423 - 600 2.7e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 715, 842
AccBSI CCGCTC 1 cut(s) 345
AccI GTMKAC 1 cut(s) 1302
AccII CGCG 2 cut(s) 1098, 1806
AciI CCGC 7 cut(s) 89, 118, 345, 687, 945, 1155, 1968
AclWI GGATC 2 cut(s) 531, 559
AcoI YGGCCR 2 cut(s) 245, 1668
AcsI RAATTY 4 cut(s) 632, 811, 1065, 2170
AcuI CTGAAG 4 cut(s) 200, 667, 1248, 1757
AcyI GRCGYC 1 cut(s) 2118
AfaI GTAC 4 cut(s) 15, 691, 1314, 2107
AfiI CCNNNNNNNGG 3 cut(s) 182, 292, 899
AflII CTTAAG 2 cut(s) 1238, 1894
AgeI ACCGGT 1 cut(s) 539
AjnI CCWGG 4 cut(s) 242, 874, 970, 1359
AjuI GAANNNNNNNTTGG 2 cut(s) 1634, 1666
Alw21I GWGCWC 2 cut(s) 711, 1060
Alw26I GTCTC 4 cut(s) 755, 1445, 1902, 2009
AlwI GGATC 2 cut(s) 531, 559
AlwNI CAGNNNCTG 1 cut(s) 1571
AoxI GGCC 7 cut(s) 3, 245, 845, 1157, 1668, 1916, 2178
ApeKI GCWGC 7 cut(s) 50, 53, 432, 446, 645, 1432, 1562
ApoI RAATTY 4 cut(s) 632, 811, 1065, 2170
AsiGI ACCGGT 1 cut(s) 539
Asp700I GAANNNNTTC 1 cut(s) 1770
AspLEI GCGC 2 cut(s) 1806, 1808
AspS9I GGNCC 7 cut(s) 253, 831, 1016, 1157, 1759, 1814, 2020
AsuC2I CCSGG 1 cut(s) 455
AsuHPI GGTGA 7 cut(s) 78, 170, 275, 308, 864, 1438, 1867
AsuII TTCGAA 1 cut(s) 201
AsuNHI GCTAGC 1 cut(s) 67
AvaII GGWCC 6 cut(s) 253, 831, 1016, 1759, 1814, 2020
BalI TGGCCA 1 cut(s) 1670
BanII GRGCYC 2 cut(s) 711, 842
BbsI GAAGAC 1 cut(s) 2139
Bbv12I GWGCWC 2 cut(s) 711, 1060
BbvCI CCTCAGC 1 cut(s) 291
BbvI GCAGC 7 cut(s) 40, 62, 419, 433, 657, 1419, 1574
BccI CCATC 8 cut(s) 241, 244, 662, 1007, 1838, 1976, 2032, 2072
BceAI ACGGC 3 cut(s) 773, 1059, 1168
BcgI CGANNNNNNTGC 6 cut(s) 425, 459, 526, 560, 1853, 1887
BciT130I CCWGG 4 cut(s) 244, 876, 972, 1361
BciVI GTATCC 2 cut(s) 393, 1745
BclI TGATCA 2 cut(s) 1372, 1857
BcnI CCSGG 1 cut(s) 455
BcoDI GTCTC 4 cut(s) 755, 1445, 1902, 2009
BfaI CTAG 6 cut(s) 68, 1191, 1436, 1469, 1733, 1946
BfmI CTRYAG 2 cut(s) 643, 849
BfrI CTTAAG 2 cut(s) 1238, 1894
BfuAI ACCTGC 2 cut(s) 715, 842
BfuI GTATCC 2 cut(s) 393, 1745
BisI GCNGC 7 cut(s) 51, 54, 433, 447, 646, 1433, 1563
BlsI GCNGC 7 cut(s) 52, 55, 434, 448, 647, 1434, 1564
Bme1390I CCNGG 5 cut(s) 244, 455, 876, 972, 1361
Bme18I GGWCC 6 cut(s) 253, 831, 1016, 1759, 1814, 2020
BmgT120I GGNCC 7 cut(s) 253, 831, 1016, 1157, 1759, 1814, 2020
BmiI GGNNCC 6 cut(s) 255, 729, 890, 1761, 1815, 2022
BmrFI CCNGG 5 cut(s) 244, 455, 876, 972, 1361
BmrI ACTGGG 1 cut(s) 1861
BmsI GCATC 2 cut(s) 162, 1880
BmtI GCTAGC 1 cut(s) 71
BmuI ACTGGG 1 cut(s) 1861
BpiI GAAGAC 1 cut(s) 2139
Bpu10I CCTNAGC 1 cut(s) 291
Bpu14I TTCGAA 1 cut(s) 201
BpuEI CTTGAG 2 cut(s) 78, 695
BpuMI CCSGG 1 cut(s) 455
BsaHI GRCGYC 1 cut(s) 2118
BsaJI CCNNGG 5 cut(s) 454, 519, 842, 1041, 1359
BsaWI WCCGGW 3 cut(s) 401, 539, 828
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 3 cut(s) 182, 292, 899
Bse118I RCCGGY 1 cut(s) 539
Bse1I ACTGG 3 cut(s) 1077, 1856, 2095
Bse3DI GCAATG 2 cut(s) 62, 1029
BseBI CCWGG 4 cut(s) 244, 876, 972, 1361
BseDI CCNNGG 5 cut(s) 454, 519, 842, 1041, 1359
BseGI GGATG 2 cut(s) 1660, 1849
BseLI CCNNNNNNNGG 3 cut(s) 182, 292, 899
BseMI GCAATG 2 cut(s) 62, 1029
BseMII CTCAG 1 cut(s) 282
BseNI ACTGG 3 cut(s) 1077, 1856, 2095
BsePI GCGCGC 1 cut(s) 1804
BseRI GAGGAG 3 cut(s) 709, 932, 1993
BseXI GCAGC 7 cut(s) 40, 62, 419, 433, 657, 1419, 1574
BseYI CCCAGC 1 cut(s) 257
BsgI GTGCAG 1 cut(s) 1757
Bsh1236I CGCG 2 cut(s) 1098, 1806
Bsh1285I CGRYCG 1 cut(s) 406
BshFI GGCC 7 cut(s) 5, 247, 847, 1159, 1670, 1918, 2180
BshTI ACCGGT 1 cut(s) 539
BsiEI CGRYCG 1 cut(s) 406
BsiHKAI GWGCWC 2 cut(s) 711, 1060
BsiSI CCGG 4 cut(s) 402, 454, 540, 829
BslFI GGGAC 2 cut(s) 1023, 1756
BslI CCNNNNNNNGG 3 cut(s) 182, 292, 899
BsmAI GTCTC 4 cut(s) 755, 1445, 1902, 2009
BsmBI CGTCTC 2 cut(s) 1902, 2009
BsmFI GGGAC 2 cut(s) 1023, 1756
BsmI GAATGC 2 cut(s) 790, 1360
BsnI GGCC 7 cut(s) 5, 247, 847, 1159, 1670, 1918, 2180
Bsp119I TTCGAA 1 cut(s) 201
Bsp1286I GDGCHC 3 cut(s) 711, 842, 1060
Bsp143I GATC 4 cut(s) 523, 564, 1372, 1857
Bsp19I CCATGG 1 cut(s) 842
Bsp68I TCGCGA 1 cut(s) 1098
BspACI CCGC 7 cut(s) 89, 118, 345, 687, 945, 1155, 1968
BspANI GGCC 7 cut(s) 5, 247, 847, 1159, 1670, 1918, 2180
BspCNI CTCAG 1 cut(s) 283
BspFNI CGCG 2 cut(s) 1098, 1806
BspHI TCATGA 2 cut(s) 309, 480
BspLI GGNNCC 6 cut(s) 255, 729, 890, 1761, 1815, 2022
BspMAI CTGCAG 2 cut(s) 647, 853
BspMI ACCTGC 2 cut(s) 715, 842
BspOI GCTAGC 1 cut(s) 71
BspPI GGATC 2 cut(s) 531, 559
BspT104I TTCGAA 1 cut(s) 201
BspTI CTTAAG 2 cut(s) 1238, 1894
BsrBI CCGCTC 1 cut(s) 345
BsrDI GCAATG 2 cut(s) 62, 1029
BsrFI RCCGGY 1 cut(s) 539
BsrI ACTGG 3 cut(s) 1077, 1856, 2095
BssAI RCCGGY 1 cut(s) 539
BssECI CCNNGG 5 cut(s) 454, 519, 842, 1041, 1359
BssHII GCGCGC 1 cut(s) 1804
BssMI GATC 4 cut(s) 523, 564, 1372, 1857
BssNI GRCGYC 1 cut(s) 2118
BssT1I CCWWGG 1 cut(s) 842
Bst2UI CCWGG 4 cut(s) 244, 876, 972, 1361
Bst4CI ACNGT 4 cut(s) 520, 592, 1055, 1650
Bst6I CTCTTC 1 cut(s) 2011
BstACI GRCGYC 1 cut(s) 2118
BstAFI CTTAAG 2 cut(s) 1238, 1894
BstAPI GCANNNNNTGC 1 cut(s) 1571
BstBI TTCGAA 1 cut(s) 201
BstC8I GCNNGC 9 cut(s) 69, 802, 849, 1048, 1157, 1802, 1806, 2122, 2126
BstDEI CTNAG 2 cut(s) 7, 291
BstDSI CCRYGG 3 cut(s) 519, 842, 1041
BstEII GGTNACC 1 cut(s) 125
BstENI CCTNNNNNAGG 1 cut(s) 290
BstF5I GGATG 2 cut(s) 1660, 1849
BstFNI CGCG 2 cut(s) 1098, 1806
BstHHI GCGC 2 cut(s) 1806, 1808
BstKTI GATC 4 cut(s) 526, 567, 1375, 1860
BstMAI GTCTC 4 cut(s) 755, 1445, 1902, 2009
BstMBI GATC 4 cut(s) 523, 564, 1372, 1857
BstMCI CGRYCG 1 cut(s) 406
BstMWI GCNNNNNNNGC 6 cut(s) 117, 806, 876, 1571, 1880, 2100
BstNI CCWGG 4 cut(s) 244, 876, 972, 1361
BstNSI RCATGY 4 cut(s) 496, 768, 804, 2128
BstPI GGTNACC 1 cut(s) 125
BstSCI CCNGG 5 cut(s) 242, 453, 874, 970, 1359
BstSFI CTRYAG 2 cut(s) 643, 849
BstUI CGCG 2 cut(s) 1098, 1806
BstV1I GCAGC 7 cut(s) 40, 62, 419, 433, 657, 1419, 1574
BstV2I GAAGAC 1 cut(s) 2139
BstX2I RGATCY 2 cut(s) 523, 564
BstXI CCANNNNNNTGG 1 cut(s) 2158
BstYI RGATCY 2 cut(s) 523, 564
BsuI GTATCC 2 cut(s) 393, 1745
BsuRI GGCC 7 cut(s) 5, 247, 847, 1159, 1670, 1918, 2180
BtgI CCRYGG 3 cut(s) 519, 842, 1041
BtgZI GCGATG 2 cut(s) 783, 2091
BtsCI GGATG 2 cut(s) 1660, 1849
BtsI GCAGTG 1 cut(s) 1218
BtsIMutI CAGTG 4 cut(s) 176, 1060, 1070, 1218
BtuMI TCGCGA 1 cut(s) 1098
BveI ACCTGC 2 cut(s) 715, 842
Cac8I GCNNGC 9 cut(s) 69, 802, 849, 1048, 1157, 1802, 1806, 2122, 2126
CaiI CAGNNNCTG 1 cut(s) 1571
CciI TCATGA 2 cut(s) 309, 480
CfoI GCGC 2 cut(s) 1806, 1808
Cfr10I RCCGGY 1 cut(s) 539
Cfr13I GGNCC 7 cut(s) 253, 831, 1016, 1157, 1759, 1814, 2020
CseI GACGC 3 cut(s) 811, 2054, 2126
Csp6I GTAC 4 cut(s) 14, 690, 1313, 2106
CspAI ACCGGT 1 cut(s) 539
CspCI CAANNNNNGTGG 2 cut(s) 1597, 1632
CviQI GTAC 4 cut(s) 14, 690, 1313, 2106
DdeI CTNAG 2 cut(s) 7, 291
DpnI GATC 4 cut(s) 525, 566, 1374, 1859
DpnII GATC 4 cut(s) 523, 564, 1372, 1857
EaeI YGGCCR 2 cut(s) 245, 1668
Eam1104I CTCTTC 1 cut(s) 2011
EarI CTCTTC 1 cut(s) 2011
Ecl136II GAGCTC 1 cut(s) 709
Eco130I CCWWGG 1 cut(s) 842
Eco147I AGGCCT 1 cut(s) 1918
Eco24I GRGCYC 2 cut(s) 711, 842
Eco32I GATATC 1 cut(s) 1344
Eco47I GGWCC 6 cut(s) 253, 831, 1016, 1759, 1814, 2020
Eco53kI GAGCTC 1 cut(s) 709
Eco57I CTGAAG 4 cut(s) 200, 667, 1248, 1757
Eco91I GGTNACC 1 cut(s) 125
EcoICRI GAGCTC 1 cut(s) 709
EcoNI CCTNNNNNAGG 1 cut(s) 290
EcoO65I GGTNACC 1 cut(s) 125
EcoRI GAATTC 1 cut(s) 632
EcoRII CCWGG 4 cut(s) 242, 874, 970, 1359
EcoRV GATATC 1 cut(s) 1344
EcoT14I CCWWGG 1 cut(s) 842
EcoT22I ATGCAT 1 cut(s) 802
EcoT38I GRGCYC 2 cut(s) 711, 842
ErhI CCWWGG 1 cut(s) 842
Esp3I CGTCTC 2 cut(s) 1902, 2009
FalI AAGNNNNNCTT 6 cut(s) 967, 999, 1191, 1223, 1221, 1253
FaqI GGGAC 2 cut(s) 1023, 1756
FauI CCCGC 2 cut(s) 938, 1148
FbaI TGATCA 2 cut(s) 1372, 1857
FblI GTMKAC 1 cut(s) 1302
Fnu4HI GCNGC 7 cut(s) 51, 54, 433, 447, 646, 1433, 1563
FokI GGATG 2 cut(s) 1667, 1856
FriOI GRGCYC 2 cut(s) 711, 842
Fsp4HI GCNGC 7 cut(s) 51, 54, 433, 447, 646, 1433, 1563
FspBI CTAG 6 cut(s) 68, 1191, 1436, 1469, 1733, 1946
GlaI GCGC 2 cut(s) 1805, 1807
GluI GCNGC 7 cut(s) 51, 54, 433, 447, 646, 1433, 1563
GsaI CCCAGC 1 cut(s) 261
HaeIII GGCC 7 cut(s) 5, 247, 847, 1159, 1670, 1918, 2180
HapII CCGG 4 cut(s) 402, 454, 540, 829
HgaI GACGC 3 cut(s) 811, 2054, 2126
HhaI GCGC 2 cut(s) 1806, 1808
Hin1I GRCGYC 1 cut(s) 2118
Hin6I GCGC 2 cut(s) 1804, 1806
HinP1I GCGC 2 cut(s) 1804, 1806
HincII GTYRAC 1 cut(s) 826
HindII GTYRAC 1 cut(s) 826
HindIII AAGCTT 1 cut(s) 1991
HinfI GANTC 1 cut(s) 2033
HpaII CCGG 4 cut(s) 402, 454, 540, 829
HphI GGTGA 7 cut(s) 78, 170, 275, 308, 864, 1438, 1867
Hpy166II GTNNAC 3 cut(s) 826, 1303, 1474
Hpy188I TCNGA 6 cut(s) 622, 1062, 1681, 1688, 1788, 2044
Hpy188III TCNNGA 9 cut(s) 310, 481, 712, 1097, 1625, 1706, 1823, 2030, 2151
Hpy8I GTNNAC 3 cut(s) 826, 1303, 1474
Hpy99I CGWCG 1 cut(s) 425
HpyAV CCTTC 8 cut(s) 155, 175, 664, 878, 1104, 1237, 1277, 2066
HpyCH4III ACNGT 4 cut(s) 520, 592, 1055, 1650
HpyCH4IV ACGT 1 cut(s) 956
HpyF10VI GCNNNNNNNGC 6 cut(s) 117, 806, 876, 1571, 1880, 2100
HpyF3I CTNAG 2 cut(s) 7, 291
HpySE526I ACGT 1 cut(s) 956
Hsp92I GRCGYC 1 cut(s) 2118
HspAI GCGC 2 cut(s) 1804, 1806
Ksp22I TGATCA 2 cut(s) 1372, 1857
Kzo9I GATC 4 cut(s) 523, 564, 1372, 1857
LmnI GCTCC 4 cut(s) 266, 721, 1688, 1942
Lsp1109I GCAGC 7 cut(s) 40, 62, 419, 433, 657, 1419, 1574
LweI GCATC 2 cut(s) 162, 1880
MaeI CTAG 6 cut(s) 68, 1191, 1436, 1469, 1733, 1946
MaeII ACGT 1 cut(s) 956
MaeIII GTNAC 5 cut(s) 125, 167, 332, 854, 977
MalI GATC 4 cut(s) 525, 566, 1374, 1859
MbiI CCGCTC 1 cut(s) 345
MboI GATC 4 cut(s) 523, 564, 1372, 1857
MboII GAAGA 8 cut(s) 1241, 1244, 1567, 1763, 1998, 1999, 2139, 2166
MfeI CAATTG 2 cut(s) 1175, 1184
MflI RGATCY 2 cut(s) 523, 564
MhlI GDGCHC 3 cut(s) 711, 842, 1060
MlsI TGGCCA 1 cut(s) 1670
MluNI TGGCCA 1 cut(s) 1670
MlyI GAGTC 1 cut(s) 2027
MmeI TCCRAC 4 cut(s) 282, 1632, 1659, 1803
Mox20I TGGCCA 1 cut(s) 1670
Mph1103I ATGCAT 1 cut(s) 802
MroXI GAANNNNTTC 1 cut(s) 1770
MscI TGGCCA 1 cut(s) 1670
MseI TTAA 6 cut(s) 351, 513, 678, 936, 1239, 1895
MslI CAYNNNNRTG 3 cut(s) 497, 1419, 1626
Msp20I TGGCCA 1 cut(s) 1670
MspA1I CMGCKG 3 cut(s) 53, 120, 687
MspCI CTTAAG 2 cut(s) 1238, 1894
MspI CCGG 4 cut(s) 402, 454, 540, 829
MspR9I CCNGG 5 cut(s) 244, 455, 876, 972, 1361
MunI CAATTG 2 cut(s) 1175, 1184
Mva1269I GAATGC 2 cut(s) 790, 1360
MvaI CCWGG 4 cut(s) 244, 876, 972, 1361
MvnI CGCG 2 cut(s) 1098, 1806
MwoI GCNNNNNNNGC 6 cut(s) 117, 806, 876, 1571, 1880, 2100
NciI CCSGG 1 cut(s) 455
NcoI CCATGG 1 cut(s) 842
NdeII GATC 4 cut(s) 523, 564, 1372, 1857
NheI GCTAGC 1 cut(s) 67
NlaIV GGNNCC 6 cut(s) 255, 729, 890, 1761, 1815, 2022
NmeAIII GCCGAG 1 cut(s) 932
NruI TCGCGA 1 cut(s) 1098
NsiI ATGCAT 1 cut(s) 802
NspI RCATGY 4 cut(s) 496, 768, 804, 2128
NspV TTCGAA 1 cut(s) 201
PaeI GCATGC 2 cut(s) 804, 2128
PagI TCATGA 2 cut(s) 309, 480
PauI GCGCGC 1 cut(s) 1804
PceI AGGCCT 1 cut(s) 1918
PctI GAATGC 2 cut(s) 790, 1360
PdmI GAANNNNTTC 1 cut(s) 1770
PinAI ACCGGT 1 cut(s) 539
PkrI GCNGC 7 cut(s) 52, 55, 434, 448, 647, 1434, 1564
PleI GAGTC 1 cut(s) 2027
PpsI GAGTC 1 cut(s) 2027
Psp124BI GAGCTC 1 cut(s) 711
Psp6I CCWGG 4 cut(s) 242, 874, 970, 1359
PspEI GGTNACC 1 cut(s) 125
PspFI CCCAGC 1 cut(s) 257
PspGI CCWGG 4 cut(s) 242, 874, 970, 1359
PspN4I GGNNCC 6 cut(s) 255, 729, 890, 1761, 1815, 2022
PspPI GGNCC 7 cut(s) 253, 831, 1016, 1157, 1759, 1814, 2020
PstI CTGCAG 2 cut(s) 647, 853
PstNI CAGNNNCTG 1 cut(s) 1571
PsuI RGATCY 2 cut(s) 523, 564
PteI GCGCGC 1 cut(s) 1804
PvuII CAGCTG 1 cut(s) 53
RruI TCGCGA 1 cut(s) 1098
RsaI GTAC 4 cut(s) 15, 691, 1314, 2107
RsaNI GTAC 4 cut(s) 14, 690, 1313, 2106
RseI CAYNNNNRTG 3 cut(s) 497, 1419, 1626
SacI GAGCTC 1 cut(s) 711
SaqAI TTAA 6 cut(s) 351, 513, 678, 936, 1239, 1895
SatI GCNGC 7 cut(s) 51, 54, 433, 447, 646, 1433, 1563
Sau3AI GATC 4 cut(s) 523, 564, 1372, 1857
Sau96I GGNCC 7 cut(s) 253, 831, 1016, 1157, 1759, 1814, 2020
SbfI CCTGCAGG 1 cut(s) 853
SchI GAGTC 1 cut(s) 2027
ScrFI CCNGG 5 cut(s) 244, 455, 876, 972, 1361
SdaI CCTGCAGG 1 cut(s) 853
SduI GDGCHC 3 cut(s) 711, 842, 1060
SfaNI GCATC 2 cut(s) 162, 1880
SfcI CTRYAG 2 cut(s) 643, 849
SfuI TTCGAA 1 cut(s) 201
SgrAI CRCCGGYG 1 cut(s) 539
SinI GGWCC 6 cut(s) 253, 831, 1016, 1759, 1814, 2020
SmiMI CAYNNNNRTG 3 cut(s) 497, 1419, 1626
SmlI CTYRAG 4 cut(s) 93, 710, 1238, 1894
SmoI CTYRAG 4 cut(s) 93, 710, 1238, 1894
SphI GCATGC 2 cut(s) 804, 2128
Sse8387I CCTGCAGG 1 cut(s) 853
SseBI AGGCCT 1 cut(s) 1918
SsiI CCGC 7 cut(s) 89, 118, 345, 687, 945, 1155, 1968
SspMI CTAG 6 cut(s) 68, 1191, 1436, 1469, 1733, 1946
SstI GAGCTC 1 cut(s) 711
StuI AGGCCT 1 cut(s) 1918
StyD4I CCNGG 5 cut(s) 242, 453, 874, 970, 1359
StyI CCWWGG 1 cut(s) 842
TaaI ACNGT 4 cut(s) 520, 592, 1055, 1650
TaiI ACGT 1 cut(s) 959
TaqI TCGA 7 cut(s) 201, 406, 507, 1317, 1624, 1863, 2163
TatI WGTACW 2 cut(s) 13, 2105
Tru1I TTAA 6 cut(s) 351, 513, 678, 936, 1239, 1895
Tru9I TTAA 6 cut(s) 351, 513, 678, 936, 1239, 1895
TscAI CASTG 4 cut(s) 183, 1060, 1077, 1218
TseI GCWGC 7 cut(s) 50, 53, 432, 446, 645, 1432, 1562
TspGWI ACGGA 5 cut(s) 253, 487, 805, 1972, 2033
TspRI CASTG 4 cut(s) 183, 1060, 1077, 1218
Vha464I CTTAAG 2 cut(s) 1238, 1894
VpaK11BI GGWCC 6 cut(s) 253, 831, 1016, 1759, 1814, 2020
XagI CCTNNNNNAGG 1 cut(s) 290
XapI RAATTY 4 cut(s) 632, 811, 1065, 2170
XceI RCATGY 4 cut(s) 496, 768, 804, 2128
XcmI CCANNNNNNNNNTGG 1 cut(s) 240
XmiI GTMKAC 1 cut(s) 1302
XmnI GAANNNNTTC 1 cut(s) 1770
XspI CTAG 6 cut(s) 68, 1191, 1436, 1469, 1733, 1946
Zsp2I ATGCAT 1 cut(s) 802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.