RchiOBHm_Chr5g0016781

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0004224
Physical Location & Seq
Reverse (-)
11541069 .. 11541467
399 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29714

Sequence Viewer

Length: 399 bp
ATGACAAGGGATCTGTTGAGACAGCAGCCAGGATCTTTACCACGGCAGACCTCAAGTAGGTCACAAACAATTAATTACCACAGAGATGGGATCCTGGGTGAAGGAGGTTACGGAACAGTTTACAAAGGAGTACTTCCGGATAATAAAGTGGTAGCCATAAAGTCCAAAATCATGGCCAAGTCCCAGACTGATCAGTTTGTTAATGAAGTAGTTGTTCTCTCTCAAATAAACCATAGAAATGTGGTGAAGCTTTTAGGTTGCTGTTTAGAGACAGAAATGCCTTTACTAGTTTATGAGTACGTTAGCAATGGCACTCTTTATGACCATGTTCATAAAAGAAATGGAAAAGGTCCACTTTCATTAGGATTGCGATTGAAGATTGCAGCAGAAACAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK hEGF

Protein Analysis

132

Amino Acids

14.64

Weight (kDa)

9.48

Isoelectric Point (pI)

36.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 26 - 113 4.5e-15 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 31 - 130 7.8e-18 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 136
AclWI GGATC 4 cut(s) 18, 40, 85, 98
AcoI YGGCCR 1 cut(s) 174
AfaI GTAC 2 cut(s) 132, 299
AfiI CCNNNNNNNGG 1 cut(s) 57
AgsI TTSAA 1 cut(s) 376
AhlI ACTAGT 1 cut(s) 286
AjnI CCWGG 2 cut(s) 28, 93
AluBI AGCT 1 cut(s) 250
AluI AGCT 1 cut(s) 250
Alw26I GTCTC 2 cut(s) 13, 263
AlwI GGATC 4 cut(s) 18, 40, 85, 98
Aor13HI TCCGGA 1 cut(s) 136
AoxI GGCC 1 cut(s) 174
ApeKI GCWGC 2 cut(s) 25, 383
AseI ATTAAT 1 cut(s) 72
AspS9I GGNCC 1 cut(s) 350
AsuHPI GGTGA 2 cut(s) 110, 256
AvaII GGWCC 1 cut(s) 350
BalI TGGCCA 1 cut(s) 176
BamHI GGATCC 1 cut(s) 90
BbvI GCAGC 2 cut(s) 37, 395
BccI CCATC 1 cut(s) 80
BceAI ACGGC 1 cut(s) 59
BciT130I CCWGG 2 cut(s) 30, 95
BclI TGATCA 1 cut(s) 190
BcoDI GTCTC 2 cut(s) 13, 263
BcuI ACTAGT 1 cut(s) 286
BfaI CTAG 1 cut(s) 287
BisI GCNGC 2 cut(s) 26, 384
BlsI GCNGC 2 cut(s) 27, 385
BmcAI AGTACT 1 cut(s) 132
Bme1390I CCNGG 2 cut(s) 30, 95
Bme18I GGWCC 1 cut(s) 350
BmgT120I GGNCC 1 cut(s) 350
BmiI GGNNCC 1 cut(s) 92
BmrFI CCNGG 2 cut(s) 30, 95
BpuEI CTTGAG 1 cut(s) 37
BsaJI CCNNGG 2 cut(s) 41, 94
BsaWI WCCGGW 1 cut(s) 136
Bsc4I CCNNNNNNNGG 1 cut(s) 57
Bse3DI GCAATG 1 cut(s) 313
BseAI TCCGGA 1 cut(s) 136
BseBI CCWGG 2 cut(s) 30, 95
BseDI CCNNGG 2 cut(s) 41, 94
BseLI CCNNNNNNNGG 1 cut(s) 57
BseMI GCAATG 1 cut(s) 313
BseXI GCAGC 2 cut(s) 37, 395
BshFI GGCC 1 cut(s) 176
BsiSI CCGG 1 cut(s) 137
BslFI GGGAC 1 cut(s) 166
BslI CCNNNNNNNGG 1 cut(s) 57
BsmAI GTCTC 2 cut(s) 13, 263
BsmFI GGGAC 1 cut(s) 166
BsnI GGCC 1 cut(s) 176
Bsp13I TCCGGA 1 cut(s) 136
Bsp143I GATC 4 cut(s) 10, 32, 90, 190
BspANI GGCC 1 cut(s) 176
BspEI TCCGGA 1 cut(s) 136
BspLI GGNNCC 1 cut(s) 92
BspPI GGATC 4 cut(s) 18, 40, 85, 98
BsrDI GCAATG 1 cut(s) 313
BssECI CCNNGG 2 cut(s) 41, 94
BssMI GATC 4 cut(s) 10, 32, 90, 190
Bst2UI CCWGG 2 cut(s) 30, 95
Bst4CI ACNGT 1 cut(s) 118
BstDSI CCRYGG 1 cut(s) 41
BstENI CCTNNNNNAGG 1 cut(s) 55
BstKTI GATC 4 cut(s) 13, 35, 93, 193
BstMAI GTCTC 2 cut(s) 13, 263
BstMBI GATC 4 cut(s) 10, 32, 90, 190
BstMWI GCNNNNNNNGC 1 cut(s) 392
BstNI CCWGG 2 cut(s) 30, 95
BstSCI CCNGG 2 cut(s) 28, 93
BstV1I GCAGC 2 cut(s) 37, 395
BstX2I RGATCY 3 cut(s) 10, 32, 90
BstXI CCANNNNNNTGG 2 cut(s) 86, 172
BstYI RGATCY 3 cut(s) 10, 32, 90
BsuRI GGCC 1 cut(s) 176
BtgI CCRYGG 1 cut(s) 41
Cfr13I GGNCC 1 cut(s) 350
Csp6I GTAC 2 cut(s) 131, 298
CviAII CATG 2 cut(s) 172, 326
CviJI RGCY 4 cut(s) 28, 155, 176, 250
CviKI_1 RGCY 4 cut(s) 28, 155, 176, 250
CviQI GTAC 2 cut(s) 131, 298
DpnI GATC 4 cut(s) 12, 34, 92, 192
DpnII GATC 4 cut(s) 10, 32, 90, 190
EaeI YGGCCR 1 cut(s) 174
Eco47I GGWCC 1 cut(s) 350
EcoNI CCTNNNNNAGG 1 cut(s) 55
EcoRII CCWGG 2 cut(s) 28, 93
FaeI CATG 2 cut(s) 175, 329
FaiI YATR 8 cut(s) 158, 173, 234, 294, 321, 327, 333, 397
FalI AAGNNNNNCTT 4 cut(s) 117, 149, 339, 371
FaqI GGGAC 1 cut(s) 166
FatI CATG 2 cut(s) 171, 325
FbaI TGATCA 1 cut(s) 190
Fnu4HI GCNGC 2 cut(s) 26, 384
Fsp4HI GCNGC 2 cut(s) 26, 384
FspBI CTAG 1 cut(s) 287
GluI GCNGC 2 cut(s) 26, 384
HaeIII GGCC 1 cut(s) 176
HapII CCGG 1 cut(s) 137
Hin1II CATG 2 cut(s) 175, 329
HindIII AAGCTT 1 cut(s) 248
HpaII CCGG 1 cut(s) 137
HphI GGTGA 2 cut(s) 110, 256
Hpy166II GTNNAC 2 cut(s) 121, 353
Hpy188III TCNNGA 1 cut(s) 137
Hpy8I GTNNAC 2 cut(s) 121, 353
HpyAV CCTTC 1 cut(s) 95
HpyCH4III ACNGT 1 cut(s) 118
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 1 cut(s) 383
HpyF10VI GCNNNNNNNGC 1 cut(s) 392
HpySE526I ACGT 1 cut(s) 300
Hsp92II CATG 2 cut(s) 175, 329
Kpn2I TCCGGA 1 cut(s) 136
Ksp22I TGATCA 1 cut(s) 190
Kzo9I GATC 4 cut(s) 10, 32, 90, 190
LpnPI CCDG 6 cut(s) 15, 42, 80, 107, 150, 197
Lsp1109I GCAGC 2 cut(s) 37, 395
MaeI CTAG 1 cut(s) 287
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 2 cut(s) 60, 107
MalI GATC 4 cut(s) 12, 34, 92, 192
MboI GATC 4 cut(s) 10, 32, 90, 190
MboII GAAGA 1 cut(s) 388
MflI RGATCY 3 cut(s) 10, 32, 90
MlsI TGGCCA 1 cut(s) 176
MluCI AATT 2 cut(s) 69, 73
MluNI TGGCCA 1 cut(s) 176
MnlI CCTC 2 cut(s) 61, 98
Mox20I TGGCCA 1 cut(s) 176
MroI TCCGGA 1 cut(s) 136
MscI TGGCCA 1 cut(s) 176
MseI TTAA 2 cut(s) 72, 201
MslI CAYNNNNRTG 2 cut(s) 84, 237
Msp20I TGGCCA 1 cut(s) 176
MspI CCGG 1 cut(s) 137
MspR9I CCNGG 2 cut(s) 30, 95
MvaI CCWGG 2 cut(s) 30, 95
MwoI GCNNNNNNNGC 1 cut(s) 392
NdeII GATC 4 cut(s) 10, 32, 90, 190
NlaIII CATG 2 cut(s) 175, 329
NlaIV GGNNCC 1 cut(s) 92
NmuCI GTSAC 1 cut(s) 60
PkrI GCNGC 2 cut(s) 27, 385
PshBI ATTAAT 1 cut(s) 72
Psp6I CCWGG 2 cut(s) 28, 93
PspGI CCWGG 2 cut(s) 28, 93
PspN4I GGNNCC 1 cut(s) 92
PspPI GGNCC 1 cut(s) 350
PsuI RGATCY 3 cut(s) 10, 32, 90
RsaI GTAC 2 cut(s) 132, 299
RsaNI GTAC 2 cut(s) 131, 298
RseI CAYNNNNRTG 2 cut(s) 84, 237
SaqAI TTAA 2 cut(s) 72, 201
SatI GCNGC 2 cut(s) 26, 384
Sau3AI GATC 4 cut(s) 10, 32, 90, 190
Sau96I GGNCC 1 cut(s) 350
ScaI AGTACT 1 cut(s) 132
ScrFI CCNGG 2 cut(s) 30, 95
SetI ASST 7 cut(s) 53, 62, 109, 252, 259, 303, 352
SinI GGWCC 1 cut(s) 350
SmiMI CAYNNNNRTG 2 cut(s) 84, 237
SmlI CTYRAG 1 cut(s) 52
SmoI CTYRAG 1 cut(s) 52
SpeI ACTAGT 1 cut(s) 286
Sse9I AATT 2 cut(s) 69, 73
SspMI CTAG 1 cut(s) 287
StyD4I CCNGG 2 cut(s) 28, 93
TaaI ACNGT 1 cut(s) 118
TaiI ACGT 1 cut(s) 303
TasI AATT 2 cut(s) 69, 73
TatI WGTACW 1 cut(s) 130
Tru1I TTAA 2 cut(s) 72, 201
Tru9I TTAA 2 cut(s) 72, 201
TseFI GTSAC 1 cut(s) 60
TseI GCWGC 2 cut(s) 25, 383
Tsp45I GTSAC 1 cut(s) 60
TspDTI ATGAA 3 cut(s) 219, 320, 348
TspGWI ACGGA 1 cut(s) 126
VpaK11BI GGWCC 1 cut(s) 350
VspI ATTAAT 1 cut(s) 72
XagI CCTNNNNNAGG 1 cut(s) 55
XspI CTAG 1 cut(s) 287
ZrmI AGTACT 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.