RchiOBHm_Chr5g0017081

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0004224
Physical Location & Seq
Reverse (-)
11750545 .. 11751435
891 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29740

Sequence Viewer

Length: 825 bp
ATGACTGTTGAACTATATATGTGCAGGAATTTCCGTAGGCCTATTTGTGATTTTATTAGTTGGAAGTTTATGGATGTATTGGGGATGAAGAAAAGGAAGTTCATAAAACTCAAAGAGAAGTACTTTACAGAAAATGGTGGTTTATTGTTAAAACAAAAACTCACTAGTCAAGGAGGATCTGTTGAGACAACCAAACTATTTACTACAAAAGAACTTGAGAAGGCCACACACAATTACCATGAAAGTAGAATCCTTGGTGAAGGAGGGTATGGAATAGTTTACAGAGGCATATTACTAGATAACCAAGTGGTTGCAATAAAAAAGTCCAAAGTTGGTGCCCCGACTCAAACTGATCAGTTTGTTAATGAGGTGATTGTTCTTTCTCAAGTCAACCATAGAAATGTAGGCACTCTCTTTGAGCACATTCACAAGAAAAAGGGTAAAAGGTCATCACTTTCATGGGAACTCCAATTGAAGATAGCATCAGAAACTGGAGGAGCACTAGCATACTTGCACTCCTCCACTTCCACACCAATCATACACCGAGATGTGAAAACAATGAATATACTATTAGATGACAATTACACAGCCAAAGTGTTAGATTTTGGAGCTTCACGTTTCATTCCTATTGATCAAACTCAACTAGCAATAATGGTGCAAGGAACATTTGGATACTTGGACCCTGAATACTTCCATTCAAATCAACTAACAGAAAAGAGTGACGTCTATAGCTTTGGAGTGGTTCTAGCAGAGCTACTCACAAGCAAAGTGGCATTTTCTTTTGCAAGGCCTAAGGCAAAGAGATGCCTTGCACACTTTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK hEGF

Protein Analysis

274

Amino Acids

31.09

Weight (kDa)

9.5

Isoelectric Point (pI)

27.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 79 - 146 5.1e-08 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 82 - 143 5.3e-06 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 136 - 254 3e-20 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 138 - 260 3.6e-24 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 726
AccB1I GGYRCC 1 cut(s) 335
AclWI GGATC 1 cut(s) 184
AcsI RAATTY 1 cut(s) 28
AcyI GRCGYC 1 cut(s) 723
AfaI GTAC 1 cut(s) 122
AgsI TTSAA 3 cut(s) 11, 475, 699
AhlI ACTAGT 1 cut(s) 164
AluBI AGCT 3 cut(s) 611, 732, 754
AluI AGCT 3 cut(s) 611, 732, 754
Alw21I GWGCWC 2 cut(s) 423, 502
Alw26I GTCTC 1 cut(s) 179
AlwI GGATC 1 cut(s) 184
AlwNI CAGNNNCTG 1 cut(s) 491
AoxI GGCC 3 cut(s) 38, 222, 788
ApoI RAATTY 1 cut(s) 28
AspS9I GGNCC 1 cut(s) 679
AsuHPI GGTGA 2 cut(s) 269, 382
AvaII GGWCC 1 cut(s) 679
AxyI CCTNAGG 1 cut(s) 792
BaeGI GKGCMC 1 cut(s) 340
BanI GGYRCC 1 cut(s) 335
Bbv12I GWGCWC 2 cut(s) 423, 502
BciVI GTATCC 1 cut(s) 665
BclI TGATCA 2 cut(s) 352, 631
BcoDI GTCTC 1 cut(s) 179
BcuI ACTAGT 1 cut(s) 164
BfaI CTAG 5 cut(s) 165, 296, 503, 644, 746
BfmI CTRYAG 1 cut(s) 727
BfuI GTATCC 1 cut(s) 665
BmcAI AGTACT 1 cut(s) 122
Bme18I GGWCC 1 cut(s) 679
BmgT120I GGNCC 1 cut(s) 679
BmiI GGNNCC 2 cut(s) 337, 681
BmsI GCATC 2 cut(s) 491, 794
BpmI CTGGAG 1 cut(s) 513
BpuEI CTTGAG 2 cut(s) 236, 369
BsaHI GRCGYC 1 cut(s) 723
BsaJI CCNNGG 1 cut(s) 253
BsaXI ACNNNNNCTCC 4 cut(s) 165, 195, 500, 530
Bse1I ACTGG 1 cut(s) 496
Bse21I CCTNAGG 1 cut(s) 792
BseDI CCNNGG 1 cut(s) 253
BseGI GGATG 2 cut(s) 79, 90
BseNI ACTGG 1 cut(s) 496
BseRI GAGGAG 2 cut(s) 508, 510
BseSI GKGCMC 1 cut(s) 340
BsgI GTGCAG 1 cut(s) 43
BshFI GGCC 3 cut(s) 40, 224, 790
BshNI GGYRCC 1 cut(s) 335
BsiHKAI GWGCWC 2 cut(s) 423, 502
BsmAI GTCTC 1 cut(s) 179
BsnI GGCC 3 cut(s) 40, 224, 790
Bsp1286I GDGCHC 3 cut(s) 340, 423, 502
Bsp143I GATC 3 cut(s) 176, 352, 631
BspANI GGCC 3 cut(s) 40, 224, 790
BspLI GGNNCC 2 cut(s) 337, 681
BspPI GGATC 1 cut(s) 184
BspT107I GGYRCC 1 cut(s) 335
BsrI ACTGG 1 cut(s) 496
BssECI CCNNGG 1 cut(s) 253
BssMI GATC 3 cut(s) 176, 352, 631
BssNI GRCGYC 1 cut(s) 723
BssT1I CCWWGG 1 cut(s) 253
Bst4CI ACNGT 1 cut(s) 7
BstACI GRCGYC 1 cut(s) 723
BstDEI CTNAG 1 cut(s) 792
BstF5I GGATG 2 cut(s) 79, 90
BstKTI GATC 3 cut(s) 179, 355, 634
BstMAI GTCTC 1 cut(s) 179
BstMBI GATC 3 cut(s) 176, 352, 631
BstSFI CTRYAG 1 cut(s) 727
BstSLI GKGCMC 1 cut(s) 340
BstX2I RGATCY 1 cut(s) 176
BstYI RGATCY 1 cut(s) 176
Bsu36I CCTNAGG 1 cut(s) 792
BsuI GTATCC 1 cut(s) 665
BsuRI GGCC 3 cut(s) 40, 224, 790
BtsCI GGATG 2 cut(s) 79, 90
CaiI CAGNNNCTG 1 cut(s) 491
Cfr13I GGNCC 1 cut(s) 679
Csp6I GTAC 1 cut(s) 121
CspCI CAANNNNNGTGG 2 cut(s) 750, 785
CviAII CATG 2 cut(s) 239, 459
CviJI RGCY 7 cut(s) 40, 224, 590, 611, 732, 754, 790
CviKI_1 RGCY 7 cut(s) 40, 224, 590, 611, 732, 754, 790
CviQI GTAC 1 cut(s) 121
DdeI CTNAG 1 cut(s) 792
DpnI GATC 3 cut(s) 178, 354, 633
DpnII GATC 3 cut(s) 176, 352, 631
Eco130I CCWWGG 1 cut(s) 253
Eco147I AGGCCT 2 cut(s) 40, 790
Eco47I GGWCC 1 cut(s) 679
Eco81I CCTNAGG 1 cut(s) 792
EcoT14I CCWWGG 1 cut(s) 253
ErhI CCWWGG 1 cut(s) 253
FaeI CATG 2 cut(s) 242, 462
FatI CATG 2 cut(s) 238, 458
FbaI TGATCA 2 cut(s) 352, 631
FokI GGATG 2 cut(s) 86, 97
FspBI CTAG 5 cut(s) 165, 296, 503, 644, 746
GsuI CTGGAG 1 cut(s) 513
HaeIII GGCC 3 cut(s) 40, 224, 790
Hin1I GRCGYC 1 cut(s) 723
Hin1II CATG 2 cut(s) 242, 462
HincII GTYRAC 1 cut(s) 391
HindII GTYRAC 1 cut(s) 391
HinfI GANTC 2 cut(s) 249, 343
HphI GGTGA 2 cut(s) 269, 382
Hpy166II GTNNAC 2 cut(s) 280, 391
Hpy188I TCNGA 1 cut(s) 487
Hpy8I GTNNAC 2 cut(s) 280, 391
HpyAV CCTTC 2 cut(s) 214, 254
HpyCH4III ACNGT 1 cut(s) 7
HpyCH4IV ACGT 2 cut(s) 616, 723
HpyCH4V TGCA 6 cut(s) 24, 314, 514, 658, 785, 812
HpyF3I CTNAG 1 cut(s) 792
HpySE526I ACGT 2 cut(s) 616, 723
Hsp92I GRCGYC 1 cut(s) 723
Hsp92II CATG 2 cut(s) 242, 462
Ksp22I TGATCA 2 cut(s) 352, 631
Kzo9I GATC 3 cut(s) 176, 352, 631
LmnI GCTCC 2 cut(s) 497, 608
LpnPI CCDG 3 cut(s) 10, 477, 696
LweI GCATC 2 cut(s) 491, 794
MaeI CTAG 5 cut(s) 165, 296, 503, 644, 746
MaeII ACGT 2 cut(s) 616, 723
MaeIII GTNAC 1 cut(s) 719
MalI GATC 3 cut(s) 178, 354, 633
MboI GATC 3 cut(s) 176, 352, 631
MboII GAAGA 2 cut(s) 100, 487
MfeI CAATTG 1 cut(s) 470
MflI RGATCY 1 cut(s) 176
MhlI GDGCHC 3 cut(s) 340, 423, 502
MluCI AATT 4 cut(s) 28, 232, 470, 580
MlyI GAGTC 1 cut(s) 337
MmeI TCCRAC 1 cut(s) 41
MnlI CCTC 6 cut(s) 167, 257, 278, 361, 488, 529
MseI TTAA 2 cut(s) 149, 363
MslI CAYNNNNRTG 3 cut(s) 399, 457, 546
MunI CAATTG 1 cut(s) 470
NdeII GATC 3 cut(s) 176, 352, 631
NlaIII CATG 2 cut(s) 242, 462
NlaIV GGNNCC 2 cut(s) 337, 681
NmuCI GTSAC 1 cut(s) 719
PceI AGGCCT 2 cut(s) 40, 790
PfeI GAWTC 1 cut(s) 249
PleI GAGTC 1 cut(s) 337
PpsI GAGTC 1 cut(s) 337
PspN4I GGNNCC 2 cut(s) 337, 681
PspPI GGNCC 1 cut(s) 679
PstNI CAGNNNCTG 1 cut(s) 491
PsuI RGATCY 1 cut(s) 176
RsaI GTAC 1 cut(s) 122
RsaNI GTAC 1 cut(s) 121
RseI CAYNNNNRTG 3 cut(s) 399, 457, 546
SaqAI TTAA 2 cut(s) 149, 363
Sau3AI GATC 3 cut(s) 176, 352, 631
Sau96I GGNCC 1 cut(s) 679
ScaI AGTACT 1 cut(s) 122
SchI GAGTC 1 cut(s) 337
SduI GDGCHC 3 cut(s) 340, 423, 502
SetI ASST 7 cut(s) 372, 449, 613, 619, 726, 734, 756
SfaNI GCATC 2 cut(s) 491, 794
SfcI CTRYAG 1 cut(s) 727
SinI GGWCC 1 cut(s) 679
SmiMI CAYNNNNRTG 3 cut(s) 399, 457, 546
SmlI CTYRAG 2 cut(s) 215, 384
SmoI CTYRAG 2 cut(s) 215, 384
SpeI ACTAGT 1 cut(s) 164
Sse9I AATT 4 cut(s) 28, 232, 470, 580
SseBI AGGCCT 2 cut(s) 40, 790
SspMI CTAG 5 cut(s) 165, 296, 503, 644, 746
StuI AGGCCT 2 cut(s) 40, 790
StyI CCWWGG 1 cut(s) 253
TaaI ACNGT 1 cut(s) 7
TaiI ACGT 2 cut(s) 619, 726
TasI AATT 4 cut(s) 28, 232, 470, 580
TatI WGTACW 1 cut(s) 120
TfiI GAWTC 1 cut(s) 249
Tru1I TTAA 2 cut(s) 149, 363
Tru9I TTAA 2 cut(s) 149, 363
TseFI GTSAC 1 cut(s) 719
Tsp45I GTSAC 1 cut(s) 719
TspDTI ATGAA 6 cut(s) 91, 101, 255, 447, 575, 610
TspGWI ACGGA 1 cut(s) 23
VpaK11BI GGWCC 1 cut(s) 679
XapI RAATTY 1 cut(s) 28
XspI CTAG 5 cut(s) 165, 296, 503, 644, 746
ZraI GACGTC 1 cut(s) 724
ZrmI AGTACT 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.