Rroxscaffold_1G00013300

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0004224
Physical Location & Seq
Forward (+)
16358197 .. 16362211
4015 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00013300.1

Sequence Viewer

Length: 744 bp
ATGCGGTTGGTACGGTTTGATTCGGGAGAATTTGCTAGCCCTAGAAGGAGAGGGCAGCAAAGCATCTTGGAAGACAAAAGGTTCAGACCTCATTCCATAGCTCTCCACTACCTTTCTTTCTCGTTGCAGCCCTCCCCACAACGGCACCTCTCGGGTGTCAATGATGTCAAAGAAGTTGCTGCTGTTGCATTAAAAAGAGAGTTCATTAAACTCAAAGAAAAGCACTTCAAAGAGAATGGTGGCTTATTGTTACTGCAACAACTCGCTAGTCATGGAAGCTCGATGAGGACAACAAAAATCTTTCCTGCTGAAGAACTTGAGAAGGCAACAAACAACTATCATGAGAGTAGAGTCCTTGGAGAAGGAGGTTATGGAACAGTTTACAAAGGAATACTGCCGGATGAAACAGTGGTTGCCATAAAGAAGTCGAAAGGTGGTGCCCTGACCCAGAGTGATCAATTTGTTAACGAGGTGATTGTACTTTCTCAAATCAACCACAGAAATGTGGTCAAGCTATTGGGTTGTTGTTTAGAAACGGAAGCCCCTTTACTAGTATACGAATTCATTACCCATGGCACTCTTTATGAGCACATTCATAAAAAAAGATCATCACTTCCATTTGAATTAAGAATGAAGATAGCAACTCAAAGCGCAGAAGCACTGTCCCACTTACACTCCTCAATTTCCACACCAATAATACATCGGGATGTGAAAACAGCAAATATTCTGATACCATTGACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

247

Amino Acids

27.75

Weight (kDa)

9.47

Isoelectric Point (pI)

50.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 113 - 244 4e-25 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 116 - 244 2.4e-28 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 144, 437
AccI GTMKAC 1 cut(s) 555
AciI CCGC 1 cut(s) 4
AcsI RAATTY 2 cut(s) 29, 560
AcuI CTGAAG 1 cut(s) 330
AfaI GTAC 2 cut(s) 12, 480
AfiI CCNNNNNNNGG 1 cut(s) 141
AgsI TTSAA 2 cut(s) 229, 623
AhlI ACTAGT 1 cut(s) 550
AluBI AGCT 3 cut(s) 101, 279, 514
AluI AGCT 3 cut(s) 101, 279, 514
Alw21I GWGCWC 1 cut(s) 591
Ama87I CYCGRG 1 cut(s) 151
ApeKI GCWGC 3 cut(s) 55, 127, 179
ApoI RAATTY 2 cut(s) 29, 560
AspLEI GCGC 1 cut(s) 653
AsuHPI GGTGA 1 cut(s) 484
AsuNHI GCTAGC 1 cut(s) 35
AvaI CYCGRG 1 cut(s) 151
BaeGI GKGCMC 1 cut(s) 442
BanI GGYRCC 2 cut(s) 144, 437
BarI GAAGNNNNNNTAC 2 cut(s) 531, 563
BbsI GAAGAC 1 cut(s) 78
Bbv12I GWGCWC 1 cut(s) 591
BbvI GCAGC 3 cut(s) 67, 139, 166
BceAI ACGGC 1 cut(s) 158
BclI TGATCA 1 cut(s) 454
BcuI ACTAGT 1 cut(s) 550
BfaI CTAG 4 cut(s) 36, 42, 267, 551
BisI GCNGC 3 cut(s) 56, 128, 180
BlsI GCNGC 3 cut(s) 57, 129, 181
BmeT110I CYCGRG 1 cut(s) 151
BmiI GGNNCC 2 cut(s) 146, 439
BmsI GCATC 1 cut(s) 72
BmtI GCTAGC 1 cut(s) 39
BpiI GAAGAC 1 cut(s) 78
BpuEI CTTGAG 1 cut(s) 338
BsaJI CCNNGG 2 cut(s) 355, 571
BsaXI ACNNNNNCTCC 2 cut(s) 659, 689
Bsc4I CCNNNNNNNGG 1 cut(s) 141
BseDI CCNNGG 2 cut(s) 355, 571
BseGI GGATG 2 cut(s) 406, 712
BseLI CCNNNNNNNGG 1 cut(s) 141
BseRI GAGGAG 1 cut(s) 667
BseSI GKGCMC 1 cut(s) 442
BseXI GCAGC 3 cut(s) 67, 139, 166
BshNI GGYRCC 2 cut(s) 144, 437
BsiHKAI GWGCWC 1 cut(s) 591
BsiHKCI CYCGRG 1 cut(s) 151
BsiSI CCGG 1 cut(s) 398
BslFI GGGAC 1 cut(s) 649
BslI CCNNNNNNNGG 1 cut(s) 141
BsmFI GGGAC 1 cut(s) 649
BsoBI CYCGRG 1 cut(s) 151
Bsp1286I GDGCHC 2 cut(s) 442, 591
Bsp143I GATC 2 cut(s) 454, 605
Bsp19I CCATGG 1 cut(s) 571
BspACI CCGC 1 cut(s) 4
BspHI TCATGA 1 cut(s) 340
BspLI GGNNCC 2 cut(s) 146, 439
BspOI GCTAGC 1 cut(s) 39
BspT107I GGYRCC 2 cut(s) 144, 437
BssECI CCNNGG 2 cut(s) 355, 571
BssMI GATC 2 cut(s) 454, 605
BssNAI GTATAC 1 cut(s) 556
BssT1I CCWWGG 2 cut(s) 355, 571
Bst1107I GTATAC 1 cut(s) 556
Bst4CI ACNGT 4 cut(s) 15, 379, 409, 663
BstC8I GCNNGC 1 cut(s) 37
BstDSI CCRYGG 1 cut(s) 571
BstF5I GGATG 2 cut(s) 406, 712
BstHHI GCGC 1 cut(s) 653
BstKTI GATC 2 cut(s) 457, 608
BstMBI GATC 2 cut(s) 454, 605
BstMWI GCNNNNNNNGC 1 cut(s) 185
BstSLI GKGCMC 1 cut(s) 442
BstV1I GCAGC 3 cut(s) 67, 139, 166
BstV2I GAAGAC 1 cut(s) 78
BstZ17I GTATAC 1 cut(s) 556
BtgI CCRYGG 1 cut(s) 571
BtsCI GGATG 2 cut(s) 406, 712
BtsIMutI CAGTG 2 cut(s) 414, 659
Cac8I GCNNGC 1 cut(s) 37
CciI TCATGA 1 cut(s) 340
CfoI GCGC 1 cut(s) 653
Csp6I GTAC 2 cut(s) 11, 479
CviAII CATG 3 cut(s) 272, 341, 572
CviJI RGCY 7 cut(s) 39, 101, 130, 243, 279, 514, 542
CviKI_1 RGCY 7 cut(s) 39, 101, 130, 243, 279, 514, 542
CviQI GTAC 2 cut(s) 11, 479
DpnI GATC 2 cut(s) 456, 607
DpnII GATC 2 cut(s) 454, 605
Eco130I CCWWGG 2 cut(s) 355, 571
Eco57I CTGAAG 1 cut(s) 330
Eco88I CYCGRG 1 cut(s) 151
EcoRI GAATTC 1 cut(s) 560
EcoT14I CCWWGG 2 cut(s) 355, 571
ErhI CCWWGG 2 cut(s) 355, 571
FaeI CATG 3 cut(s) 275, 344, 575
FaqI GGGAC 1 cut(s) 649
FatI CATG 3 cut(s) 271, 340, 571
FbaI TGATCA 1 cut(s) 454
FblI GTMKAC 1 cut(s) 555
Fnu4HI GCNGC 3 cut(s) 56, 128, 180
FokI GGATG 2 cut(s) 413, 719
Fsp4HI GCNGC 3 cut(s) 56, 128, 180
FspBI CTAG 4 cut(s) 36, 42, 267, 551
GlaI GCGC 1 cut(s) 652
GluI GCNGC 3 cut(s) 56, 128, 180
HapII CCGG 1 cut(s) 398
HhaI GCGC 1 cut(s) 653
Hin1II CATG 3 cut(s) 275, 344, 575
Hin6I GCGC 1 cut(s) 651
HinP1I GCGC 1 cut(s) 651
HincII GTYRAC 1 cut(s) 466
HindII GTYRAC 1 cut(s) 466
HinfI GANTC 2 cut(s) 20, 351
HpaI GTTAAC 1 cut(s) 466
HpaII CCGG 1 cut(s) 398
HphI GGTGA 1 cut(s) 484
Hpy166II GTNNAC 3 cut(s) 382, 466, 556
Hpy188I TCNGA 2 cut(s) 86, 729
Hpy188III TCNNGA 3 cut(s) 24, 341, 704
Hpy8I GTNNAC 3 cut(s) 382, 466, 556
HpyAV CCTTC 3 cut(s) 39, 316, 356
HpyCH4III ACNGT 4 cut(s) 15, 379, 409, 663
HpyCH4V TGCA 3 cut(s) 127, 188, 256
HpyF10VI GCNNNNNNNGC 1 cut(s) 185
Hsp92II CATG 3 cut(s) 275, 344, 575
HspAI GCGC 1 cut(s) 651
Ksp22I TGATCA 1 cut(s) 454
KspAI GTTAAC 1 cut(s) 466
Kzo9I GATC 2 cut(s) 454, 605
LpnPI CCDG 4 cut(s) 318, 411, 455, 461
Lsp1109I GCAGC 3 cut(s) 67, 139, 166
LweI GCATC 1 cut(s) 72
MaeI CTAG 4 cut(s) 36, 42, 267, 551
MaeIII GTNAC 1 cut(s) 249
MalI GATC 2 cut(s) 456, 607
MboI GATC 2 cut(s) 454, 605
MboII GAAGA 3 cut(s) 83, 323, 646
MhlI GDGCHC 2 cut(s) 442, 591
MluCI AATT 5 cut(s) 29, 458, 560, 623, 681
MlyI GAGTC 1 cut(s) 360
MnlI CCTC 8 cut(s) 44, 99, 142, 158, 279, 359, 463, 688
MseI TTAA 4 cut(s) 191, 207, 465, 626
MslI CAYNNNNRTG 2 cut(s) 501, 705
MspI CCGG 1 cut(s) 398
MwoI GCNNNNNNNGC 1 cut(s) 185
NcoI CCATGG 1 cut(s) 571
NdeII GATC 2 cut(s) 454, 605
NheI GCTAGC 1 cut(s) 35
NlaIII CATG 3 cut(s) 275, 344, 575
NlaIV GGNNCC 2 cut(s) 146, 439
PagI TCATGA 1 cut(s) 340
PfeI GAWTC 1 cut(s) 20
PkrI GCNGC 3 cut(s) 57, 129, 181
PleI GAGTC 1 cut(s) 359
PpsI GAGTC 1 cut(s) 359
PspN4I GGNNCC 2 cut(s) 146, 439
RsaI GTAC 2 cut(s) 12, 480
RsaNI GTAC 2 cut(s) 11, 479
RseI CAYNNNNRTG 2 cut(s) 501, 705
SaqAI TTAA 4 cut(s) 191, 207, 465, 626
SatI GCNGC 3 cut(s) 56, 128, 180
Sau3AI GATC 2 cut(s) 454, 605
SchI GAGTC 1 cut(s) 360
SduI GDGCHC 2 cut(s) 442, 591
SfaNI GCATC 1 cut(s) 72
SmiMI CAYNNNNRTG 2 cut(s) 501, 705
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
SpeI ACTAGT 1 cut(s) 550
Sse9I AATT 5 cut(s) 29, 458, 560, 623, 681
SsiI CCGC 1 cut(s) 4
SspI AATATT 1 cut(s) 724
SspMI CTAG 4 cut(s) 36, 42, 267, 551
StyI CCWWGG 2 cut(s) 355, 571
TaaI ACNGT 4 cut(s) 15, 379, 409, 663
TaqI TCGA 2 cut(s) 281, 428
TasI AATT 5 cut(s) 29, 458, 560, 623, 681
TatI WGTACW 1 cut(s) 478
TfiI GAWTC 1 cut(s) 20
Tru1I TTAA 4 cut(s) 191, 207, 465, 626
Tru9I TTAA 4 cut(s) 191, 207, 465, 626
TscAI CASTG 2 cut(s) 414, 666
TseI GCWGC 3 cut(s) 55, 127, 179
TspDTI ATGAA 5 cut(s) 193, 417, 553, 584, 647
TspGWI ACGGA 1 cut(s) 551
TspRI CASTG 2 cut(s) 414, 666
XapI RAATTY 2 cut(s) 29, 560
XmiI GTMKAC 1 cut(s) 555
XspI CTAG 4 cut(s) 36, 42, 267, 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.