RLG00000029879

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004224
Physical Location & Seq
Forward (+)
50644066 .. 50650936
6871 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029879

Sequence Viewer

Length: 2322 bp
ATGGCCTTGGTACATGGGAAAATGCTTCTTATCAAACTCTCATTAGCCTTATTAGCAGCTGCAACAACATTGCTAGCTGATAAACCCCCCCCACCGCCTCAAGCCAAGCCCAACTGCCCAGACCACTGTGGTGACCTCACAATTCCATATCCATTTGGCATCGGTGATGGCTGTTACATACCACTGAAGGGACAGGAAAAGCAATTCGAACTCACTTGTAATCATTCTACTAAAAACCCATCATTGACCTGGACGTACCCGGAGTCCGTCACTATCTCAGGTTTTCACCTTGCTGAGGGTGAGTTGGAAGTCATGAATGGGATAAGCAAGGATTGTTACAACAAGAGCGGTTTTAATGCAAAATTACTTCGTGATGTCGCTTGGTATCCTCCTTTCAGCGTTTCCGGTCAAAAAAACAGCTTCGTTGCCATAGGCTGCGACACTGCTGCTGCTTTCCGGGGTTTTCGTGGCGAAAAAATAATCATGACGGGGTGCATGTCCATGTGCCTCGACATTAACACCGTGGATACAAACTCTTGCACTGGTATCGGGTGTTGCCAAACCAAAATCTCTGATGGACTGAATAATATTACTGTGAGATTGGATAGCTATTACTATCATTTGGATGTATGGGAATTTAATCCCTGCAGCTATGCCTTCGTTGTGAAAGATGGCTACTTTCACTTCAGGGGTTCGAGGGGTTTCGAAGAGCTCAAGAACAAAGATGAGATTCCGATGATCGTTAACTGGCAGATTGGAACTGAGACATGCGATGTAGCTAAAAAGAATGCCGTGCAGTATGCATGCAAGGCAAATTCCGTGTGTGTTAACCGGACCAAGAGCCCATGGCCTGCAGGTTACTATTGCCAATGCTCTCCAGGCTACGAAGGGAACCCATACATCGGTTGCCAAGATATTGACGAGTGTTTGGCTGAAATTAACCCATGCGGGAATGAAACGTGCAAAAATTTACCTGGAAGTTACTCTTGTAAATGTAGTAAAGGGTTCACAAATGATGGTCCTTTGAGGTGCATTGCTCGTCCCACAGCAAACAACACTCCCCTGAAAATTTCATTGGGTGTTTGTATAGGCTTCTTTGTTCTATTAGTCGTAATTTTTGGGTTATATCGCAAATTCAAGAGAAGACAGTTCAACGAATTGAAAAAAAAGTACTTTGAAGCAAACGGCGGGCCAAAATTACAACTACAATTGAAGCAATTGGCTGGTCAAAGAGAATCTCTTGCAGCGCAAATCTTTACTGAAGAAGAACTTAAGAAGGCAACAAAGAATTACAATGAAAATGAAAAAATTGGTGAAGGAGGCTATGCAATAGTCTACAAAGGCGAACTCGATGATAAAAGAGTGGTTGCAATAAAGATGTCCAAAATGACTGCCCAGGTCAGAAGTGATCAGTTTATCAATGAGGTCATTGTTCTTTCTCAAATCAGTCATAGAAATGTGGTGAGGCTGCTAGGCTGTTGTTTGGAGACAAAAACGCCTATTCTAGTTTACGAGTCTGTTGTTAATGGAACTCTTTATGATCACATTCATAAAAAGAAAGGACAACCACTTTCACTTGCACAACGATTGAAGATAGCAGCAGAAACTGCGACGGCACTTTCATACTTACATCACTCCACCACCATGAAAATTGTTCATCGAGATGTCAAGGCAACAAATATACTGTTGGATGAGAAACTTGCGGCCAAAGTGTCGGACTTCGGAGCTTCAAAACTGGTTTCTGACGACAAAGCTCAACTTTCAACTTTAGTGCAGGGTACAAGGGGATACTTGGACCCTGAATATCTTCAGTCCAACACACTAACAGAAAAGAGTGACGTTTATAGGTTTGGAGTTGTCCTCGTGGAGCTACTGACAAGCGAGAATGCATTTAGTTTCGATAAACCCGAGGCGGAGAGAAACCTGGCTAACGTCTTCGTTTCTACAATTGAAAATAAAGGTCCTCTGGATCCAATTCTCGATGGTGAAATAGTCAAGGATGGAAATCGGGAGATCATTGAAAAAGTTGCCAAGCTCGCCGGAAGATGCTTAAGTGTGAAAGGAGACGAAAGGCCTACCATGAAAGAAGTTGAGAAGGAGCTAGATGAAATACTGAAAACTTTGGCAAAGCAACCAGGGGGAAAGAAGCTTTATGCCTCCTCTTCCAAGGAGACGGACCCCTCTCTTGAGTCACATTCAGACGCTTACAATGTGGAAATTAGTGATGAAGGCGATGGTGGTTCTACTGGCATCATTAGCAGTGCAGAGTATGACGCCAGCATGCAAAATCAAGCCCAAAAGCCATCCGGTCATGGTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK cEGF

Protein Analysis

774

Amino Acids

85.26

Weight (kDa)

7.13

Isoelectric Point (pI)

30.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 39 - 104 2e-09 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 305 - 344 5.1e-08 Calcium-binding EGF domain
Pkinase PF00069 431 - 698 2.6e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 434 - 701 1.4e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 845
AccBSI CCGCTC 1 cut(s) 348
AccI GTMKAC 1 cut(s) 1335
AciI CCGC 6 cut(s) 95, 348, 948, 1188, 1703, 1913
AclWI GGATC 2 cut(s) 1964, 1977
AcoI YGGCCR 1 cut(s) 1704
AcsI RAATTY 5 cut(s) 635, 814, 967, 1068, 1133
AcuI CTGAAG 4 cut(s) 206, 670, 1281, 1793
AcyI GRCGYC 1 cut(s) 2274
AfaI GTAC 4 cut(s) 12, 257, 1172, 1780
AfiI CCNNNNNNNGG 3 cut(s) 188, 295, 902
AflII CTTAAG 2 cut(s) 1271, 2050
AjnI CCWGG 6 cut(s) 248, 877, 973, 1395, 1923, 2134
AjuI GAANNNNNNNTTGG 2 cut(s) 1058, 1090
AleI CACNNNNGTG 1 cut(s) 129
Alw21I GWGCWC 1 cut(s) 714
Alw26I GTCTC 4 cut(s) 758, 1481, 2058, 2165
AlwI GGATC 2 cut(s) 1964, 1977
AlwNI CAGNNNCTG 1 cut(s) 1607
Ama87I CYCGRG 1 cut(s) 1907
AoxI GGCC 5 cut(s) 3, 848, 1190, 1704, 2072
ApeKI GCWGC 9 cut(s) 56, 59, 435, 446, 449, 648, 1244, 1468, 1598
ApoI RAATTY 5 cut(s) 635, 814, 967, 1068, 1133
Asp700I GAANNNNTTC 1 cut(s) 1806
AspLEI GCGC 1 cut(s) 1249
AspS9I GGNCC 6 cut(s) 834, 1019, 1190, 1795, 1961, 2176
AsuC2I CCSGG 2 cut(s) 260, 458
AsuHPI GGTGA 7 cut(s) 143, 176, 278, 311, 1325, 1474, 1997
AsuII TTCGAA 2 cut(s) 207, 705
AsuNHI GCTAGC 1 cut(s) 73
AvaI CYCGRG 1 cut(s) 1907
AvaII GGWCC 5 cut(s) 834, 1019, 1795, 1961, 2176
BamHI GGATCC 1 cut(s) 1969
BanII GRGCYC 2 cut(s) 714, 845
BauI CACGAG 1 cut(s) 1862
BbsI GAAGAC 2 cut(s) 1150, 1927
Bbv12I GWGCWC 1 cut(s) 714
BbvCI CCTCAGC 1 cut(s) 294
BbvI GCAGC 9 cut(s) 46, 68, 422, 433, 436, 660, 1256, 1455, 1610
BccI CCATC 9 cut(s) 161, 247, 569, 665, 1010, 1976, 1994, 2228, 2311
BceAI ACGGC 3 cut(s) 776, 1201, 1629
BcgI CGANNNNNNTGC 4 cut(s) 428, 462, 529, 563
BciT130I CCWGG 6 cut(s) 250, 879, 975, 1397, 1925, 2136
BciVI GTATCC 3 cut(s) 396, 520, 1781
BclI TGATCA 2 cut(s) 1408, 1540
BcnI CCSGG 2 cut(s) 260, 458
BcoDI GTCTC 4 cut(s) 758, 1481, 2058, 2165
BfaI CTAG 4 cut(s) 74, 1472, 1505, 2102
BfmI CTRYAG 2 cut(s) 646, 852
BfrI CTTAAG 2 cut(s) 1271, 2050
BfuAI ACCTGC 1 cut(s) 845
BfuI GTATCC 3 cut(s) 396, 520, 1781
BmcAI AGTACT 1 cut(s) 1172
Bme1390I CCNGG 8 cut(s) 250, 260, 458, 879, 975, 1397, 1925, 2136
Bme18I GGWCC 5 cut(s) 834, 1019, 1795, 1961, 2176
BmeT110I CYCGRG 1 cut(s) 1907
BmgT120I GGNCC 6 cut(s) 834, 1019, 1190, 1795, 1961, 2176
BmiI GGNNCC 4 cut(s) 893, 1797, 1971, 2178
BmrFI CCNGG 8 cut(s) 250, 260, 458, 879, 975, 1397, 1925, 2136
BmsI GCATC 3 cut(s) 168, 2036, 2259
BmtI GCTAGC 1 cut(s) 77
BpiI GAAGAC 2 cut(s) 1150, 1927
BplI GAGNNNNNCTC 2 cut(s) 1845, 1877
BpmI CTGGAG 1 cut(s) 861
Bpu10I CCTNAGC 1 cut(s) 294
Bpu14I TTCGAA 2 cut(s) 207, 705
BpuEI CTTGAG 3 cut(s) 84, 698, 2207
BpuMI CCSGG 2 cut(s) 260, 458
BsaBI GATNNNNATC 1 cut(s) 2004
BsaHI GRCGYC 1 cut(s) 2274
BsaJI CCNNGG 8 cut(s) 6, 457, 522, 845, 1395, 1908, 2135, 2166
BsaWI WCCGGW 3 cut(s) 404, 831, 2306
Bsc4I CCNNNNNNNGG 3 cut(s) 188, 295, 902
Bse1I ACTGG 4 cut(s) 547, 752, 1740, 2251
Bse3DI GCAATG 2 cut(s) 68, 1032
Bse8I GATNNNNATC 1 cut(s) 2004
BseBI CCWGG 6 cut(s) 250, 879, 975, 1397, 1925, 2136
BseDI CCNNGG 8 cut(s) 6, 457, 522, 845, 1395, 1908, 2135, 2166
BseGI GGATG 4 cut(s) 631, 1696, 2005, 2303
BseJI GATNNNNATC 1 cut(s) 2004
BseLI CCNNNNNNNGG 3 cut(s) 188, 295, 902
BseMI GCAATG 2 cut(s) 68, 1032
BseMII CTCAG 3 cut(s) 285, 291, 753
BseNI ACTGG 4 cut(s) 547, 752, 1740, 2251
BseRI GAGGAG 1 cut(s) 2149
BseXI GCAGC 9 cut(s) 46, 68, 422, 433, 436, 660, 1256, 1455, 1610
BsgI GTGCAG 3 cut(s) 815, 1793, 2283
BshFI GGCC 5 cut(s) 5, 850, 1192, 1706, 2074
BsiHKAI GWGCWC 1 cut(s) 714
BsiHKCI CYCGRG 1 cut(s) 1907
BsiSI CCGG 6 cut(s) 260, 405, 457, 832, 2040, 2307
BslFI GGGAC 2 cut(s) 204, 1026
BslI CCNNNNNNNGG 3 cut(s) 188, 295, 902
BsmAI GTCTC 4 cut(s) 758, 1481, 2058, 2165
BsmBI CGTCTC 2 cut(s) 2058, 2165
BsmFI GGGAC 2 cut(s) 204, 1026
BsmI GAATGC 2 cut(s) 793, 1891
BsnI GGCC 5 cut(s) 5, 850, 1192, 1706, 2074
BsoBI CYCGRG 1 cut(s) 1907
Bsp119I TTCGAA 2 cut(s) 207, 705
Bsp1286I GDGCHC 2 cut(s) 714, 845
Bsp143I GATC 5 cut(s) 738, 1408, 1540, 1969, 2013
Bsp19I CCATGG 1 cut(s) 845
BspACI CCGC 6 cut(s) 95, 348, 948, 1188, 1703, 1913
BspANI GGCC 5 cut(s) 5, 850, 1192, 1706, 2074
BspCNI CTCAG 3 cut(s) 286, 290, 754
BspHI TCATGA 2 cut(s) 312, 483
BspLI GGNNCC 4 cut(s) 893, 1797, 1971, 2178
BspMAI CTGCAG 2 cut(s) 650, 856
BspMI ACCTGC 1 cut(s) 845
BspOI GCTAGC 1 cut(s) 77
BspPI GGATC 2 cut(s) 1964, 1977
BspQI GCTCTTC 1 cut(s) 702
BspT104I TTCGAA 2 cut(s) 207, 705
BspTI CTTAAG 2 cut(s) 1271, 2050
BsrBI CCGCTC 1 cut(s) 348
BsrDI GCAATG 2 cut(s) 68, 1032
BsrI ACTGG 4 cut(s) 547, 752, 1740, 2251
BssECI CCNNGG 8 cut(s) 6, 457, 522, 845, 1395, 1908, 2135, 2166
BssMI GATC 5 cut(s) 738, 1408, 1540, 1969, 2013
BssNI GRCGYC 1 cut(s) 2274
BssSI CACGAG 1 cut(s) 1862
BssT1I CCWWGG 3 cut(s) 6, 845, 2166
Bst2BI CACGAG 1 cut(s) 1862
Bst2UI CCWGG 6 cut(s) 250, 879, 975, 1397, 1925, 2136
Bst4CI ACNGT 5 cut(s) 128, 523, 595, 1149, 1686
Bst6I CTCTTC 2 cut(s) 702, 2167
BstACI GRCGYC 1 cut(s) 2274
BstAFI CTTAAG 2 cut(s) 1271, 2050
BstAPI GCANNNNNTGC 1 cut(s) 1607
BstBI TTCGAA 2 cut(s) 207, 705
BstC8I GCNNGC 7 cut(s) 75, 805, 852, 1190, 2037, 2278, 2282
BstDEI CTNAG 3 cut(s) 277, 294, 762
BstDSI CCRYGG 2 cut(s) 522, 845
BstEII GGTNACC 1 cut(s) 131
BstENI CCTNNNNNAGG 1 cut(s) 293
BstF5I GGATG 4 cut(s) 631, 1696, 2005, 2303
BstHHI GCGC 1 cut(s) 1249
BstKTI GATC 5 cut(s) 741, 1411, 1543, 1972, 2016
BstMAI GTCTC 4 cut(s) 758, 1481, 2058, 2165
BstMBI GATC 5 cut(s) 738, 1408, 1540, 1969, 2013
BstMWI GCNNNNNNNGC 6 cut(s) 53, 809, 879, 1607, 2036, 2256
BstNI CCWGG 6 cut(s) 250, 879, 975, 1397, 1925, 2136
BstNSI RCATGY 4 cut(s) 499, 771, 807, 2284
BstPI GGTNACC 1 cut(s) 131
BstSCI CCNGG 8 cut(s) 248, 258, 456, 877, 973, 1395, 1923, 2134
BstSFI CTRYAG 2 cut(s) 646, 852
BstV1I GCAGC 9 cut(s) 46, 68, 422, 433, 436, 660, 1256, 1455, 1610
BstV2I GAAGAC 2 cut(s) 1150, 1927
BstX2I RGATCY 1 cut(s) 1969
BstYI RGATCY 1 cut(s) 1969
BsuI GTATCC 3 cut(s) 396, 520, 1781
BsuRI GGCC 5 cut(s) 5, 850, 1192, 1706, 2074
BtgI CCRYGG 2 cut(s) 522, 845
BtgZI GCGATG 2 cut(s) 786, 2247
BtsCI GGATG 4 cut(s) 631, 1696, 2005, 2303
BtsI GCAGTG 2 cut(s) 441, 2266
BtsIMutI CAGTG 5 cut(s) 124, 182, 441, 540, 2266
BveI ACCTGC 1 cut(s) 845
Cac8I GCNNGC 7 cut(s) 75, 805, 852, 1190, 2037, 2278, 2282
CaiI CAGNNNCTG 1 cut(s) 1607
CciI TCATGA 2 cut(s) 312, 483
CfoI GCGC 1 cut(s) 1249
Cfr13I GGNCC 6 cut(s) 834, 1019, 1190, 1795, 1961, 2176
CseI GACGC 2 cut(s) 2210, 2282
Csp6I GTAC 4 cut(s) 11, 256, 1171, 1779
CviQI GTAC 4 cut(s) 11, 256, 1171, 1779
DdeI CTNAG 3 cut(s) 277, 294, 762
DpnI GATC 5 cut(s) 740, 1410, 1542, 1971, 2015
DpnII GATC 5 cut(s) 738, 1408, 1540, 1969, 2013
EaeI YGGCCR 1 cut(s) 1704
Eam1104I CTCTTC 2 cut(s) 702, 2167
EarI CTCTTC 2 cut(s) 702, 2167
EciI GGCGGA 1 cut(s) 1928
Ecl136II GAGCTC 1 cut(s) 712
Eco130I CCWWGG 3 cut(s) 6, 845, 2166
Eco147I AGGCCT 1 cut(s) 2074
Eco24I GRGCYC 2 cut(s) 714, 845
Eco47I GGWCC 5 cut(s) 834, 1019, 1795, 1961, 2176
Eco53kI GAGCTC 1 cut(s) 712
Eco57I CTGAAG 4 cut(s) 206, 670, 1281, 1793
Eco88I CYCGRG 1 cut(s) 1907
Eco91I GGTNACC 1 cut(s) 131
EcoICRI GAGCTC 1 cut(s) 712
EcoNI CCTNNNNNAGG 1 cut(s) 293
EcoO109I RGGNCCY 1 cut(s) 1961
EcoO65I GGTNACC 1 cut(s) 131
EcoRII CCWGG 6 cut(s) 248, 877, 973, 1395, 1923, 2134
EcoT14I CCWWGG 3 cut(s) 6, 845, 2166
EcoT22I ATGCAT 2 cut(s) 805, 1891
EcoT38I GRGCYC 2 cut(s) 714, 845
ErhI CCWWGG 3 cut(s) 6, 845, 2166
Esp3I CGTCTC 2 cut(s) 2058, 2165
FalI AAGNNNNNCTT 6 cut(s) 970, 1002, 1254, 1286, 1743, 1775
FaqI GGGAC 2 cut(s) 204, 1026
FauI CCCGC 2 cut(s) 941, 1181
FbaI TGATCA 2 cut(s) 1408, 1540
FblI GTMKAC 1 cut(s) 1335
FokI GGATG 4 cut(s) 638, 1703, 2012, 2290
FriOI GRGCYC 2 cut(s) 714, 845
FspBI CTAG 4 cut(s) 74, 1472, 1505, 2102
GlaI GCGC 1 cut(s) 1248
GsuI CTGGAG 1 cut(s) 861
HaeIII GGCC 5 cut(s) 5, 850, 1192, 1706, 2074
HapII CCGG 6 cut(s) 260, 405, 457, 832, 2040, 2307
HgaI GACGC 2 cut(s) 2210, 2282
HhaI GCGC 1 cut(s) 1249
Hin1I GRCGYC 1 cut(s) 2274
Hin6I GCGC 1 cut(s) 1247
HinP1I GCGC 1 cut(s) 1247
HincII GTYRAC 2 cut(s) 745, 829
HindII GTYRAC 2 cut(s) 745, 829
HindIII AAGCTT 1 cut(s) 2147
HinfI GANTC 5 cut(s) 263, 730, 1235, 1514, 2189
HpaI GTTAAC 2 cut(s) 745, 829
HpaII CCGG 6 cut(s) 260, 405, 457, 832, 2040, 2307
HphI GGTGA 7 cut(s) 143, 176, 278, 311, 1325, 1474, 1997
Hpy166II GTNNAC 5 cut(s) 745, 829, 1008, 1336, 1510
Hpy188I TCNGA 7 cut(s) 574, 735, 1403, 1717, 1724, 1744, 2200
Hpy8I GTNNAC 5 cut(s) 745, 829, 1008, 1336, 1510
Hpy99I CGWCG 1 cut(s) 1615
HpyAV CCTTC 7 cut(s) 181, 667, 881, 1270, 1310, 2089, 2222
HpyCH4III ACNGT 5 cut(s) 128, 523, 595, 1149, 1686
HpyCH4IV ACGT 4 cut(s) 254, 959, 1839, 1932
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 809, 879, 1607, 2036, 2256
HpyF3I CTNAG 3 cut(s) 277, 294, 762
HpySE526I ACGT 4 cut(s) 254, 959, 1839, 1932
Hsp92I GRCGYC 1 cut(s) 2274
HspAI GCGC 1 cut(s) 1247
Ksp22I TGATCA 2 cut(s) 1408, 1540
KspAI GTTAAC 2 cut(s) 745, 829
Kzo9I GATC 5 cut(s) 738, 1408, 1540, 1969, 2013
LguI GCTCTTC 1 cut(s) 702
LmnI GCTCC 3 cut(s) 1724, 1867, 2098
Lsp1109I GCAGC 9 cut(s) 46, 68, 422, 433, 436, 660, 1256, 1455, 1610
LweI GCATC 3 cut(s) 168, 2036, 2259
MaeI CTAG 4 cut(s) 74, 1472, 1505, 2102
MaeII ACGT 4 cut(s) 254, 959, 1839, 1932
MaeIII GTNAC 8 cut(s) 131, 173, 268, 335, 857, 980, 1835, 2190
MalI GATC 5 cut(s) 740, 1410, 1542, 1971, 2015
MbiI CCGCTC 1 cut(s) 348
MboI GATC 5 cut(s) 738, 1408, 1540, 1969, 2013
MboII GAAGA 9 cut(s) 719, 1155, 1274, 1277, 1603, 1799, 1927, 2055, 2154
MfeI CAATTG 3 cut(s) 1208, 1217, 1947
MflI RGATCY 1 cut(s) 1969
MhlI GDGCHC 2 cut(s) 714, 845
MlyI GAGTC 3 cut(s) 272, 1523, 2198
MmeI TCCRAC 4 cut(s) 285, 1668, 1695, 1839
Mph1103I ATGCAT 2 cut(s) 805, 1891
MroXI GAANNNNTTC 1 cut(s) 1806
MseI TTAA 9 cut(s) 354, 516, 639, 744, 828, 939, 1272, 1524, 2051
MslI CAYNNNNRTG 6 cut(s) 129, 500, 624, 1455, 1643, 1662
MspA1I CMGCKG 1 cut(s) 59
MspCI CTTAAG 2 cut(s) 1271, 2050
MspI CCGG 6 cut(s) 260, 405, 457, 832, 2040, 2307
MspR9I CCNGG 8 cut(s) 250, 260, 458, 879, 975, 1397, 1925, 2136
MunI CAATTG 3 cut(s) 1208, 1217, 1947
Mva1269I GAATGC 2 cut(s) 793, 1891
MvaI CCWGG 6 cut(s) 250, 879, 975, 1397, 1925, 2136
MwoI GCNNNNNNNGC 6 cut(s) 53, 809, 879, 1607, 2036, 2256
NciI CCSGG 2 cut(s) 260, 458
NcoI CCATGG 1 cut(s) 845
NdeII GATC 5 cut(s) 738, 1408, 1540, 1969, 2013
NheI GCTAGC 1 cut(s) 73
NlaIV GGNNCC 4 cut(s) 893, 1797, 1971, 2178
NmuCI GTSAC 4 cut(s) 131, 268, 1835, 2190
NsiI ATGCAT 2 cut(s) 805, 1891
NspI RCATGY 4 cut(s) 499, 771, 807, 2284
NspV TTCGAA 2 cut(s) 207, 705
OliI CACNNNNGTG 1 cut(s) 129
PaeI GCATGC 2 cut(s) 807, 2284
PagI TCATGA 2 cut(s) 312, 483
PceI AGGCCT 1 cut(s) 2074
PciSI GCTCTTC 1 cut(s) 702
PcsI WCGNNNNNNNCGW 1 cut(s) 1905
PctI GAATGC 2 cut(s) 793, 1891
PdmI GAANNNNTTC 1 cut(s) 1806
PfeI GAWTC 2 cut(s) 730, 1235
PleI GAGTC 3 cut(s) 271, 1522, 2197
PpsI GAGTC 3 cut(s) 271, 1522, 2197
PpuMI RGGWCCY 1 cut(s) 1961
Psp124BI GAGCTC 1 cut(s) 714
Psp5II RGGWCCY 1 cut(s) 1961
Psp6I CCWGG 6 cut(s) 248, 877, 973, 1395, 1923, 2134
PspEI GGTNACC 1 cut(s) 131
PspGI CCWGG 6 cut(s) 248, 877, 973, 1395, 1923, 2134
PspN4I GGNNCC 4 cut(s) 893, 1797, 1971, 2178
PspPI GGNCC 6 cut(s) 834, 1019, 1190, 1795, 1961, 2176
PspPPI RGGWCCY 1 cut(s) 1961
PstI CTGCAG 2 cut(s) 650, 856
PstNI CAGNNNCTG 1 cut(s) 1607
PsuI RGATCY 1 cut(s) 1969
PvuII CAGCTG 1 cut(s) 59
RsaI GTAC 4 cut(s) 12, 257, 1172, 1780
RsaNI GTAC 4 cut(s) 11, 256, 1171, 1779
RseI CAYNNNNRTG 6 cut(s) 129, 500, 624, 1455, 1643, 1662
SacI GAGCTC 1 cut(s) 714
SapI GCTCTTC 1 cut(s) 702
SaqAI TTAA 9 cut(s) 354, 516, 639, 744, 828, 939, 1272, 1524, 2051
Sau3AI GATC 5 cut(s) 738, 1408, 1540, 1969, 2013
Sau96I GGNCC 6 cut(s) 834, 1019, 1190, 1795, 1961, 2176
SbfI CCTGCAGG 1 cut(s) 856
ScaI AGTACT 1 cut(s) 1172
SchI GAGTC 3 cut(s) 272, 1523, 2198
ScrFI CCNGG 8 cut(s) 250, 260, 458, 879, 975, 1397, 1925, 2136
SdaI CCTGCAGG 1 cut(s) 856
SduI GDGCHC 2 cut(s) 714, 845
SfaNI GCATC 3 cut(s) 168, 2036, 2259
SfcI CTRYAG 2 cut(s) 646, 852
SfuI TTCGAA 2 cut(s) 207, 705
SinI GGWCC 5 cut(s) 834, 1019, 1795, 1961, 2176
SmiMI CAYNNNNRTG 6 cut(s) 129, 500, 624, 1455, 1643, 1662
SmlI CTYRAG 5 cut(s) 99, 713, 1271, 2050, 2186
SmoI CTYRAG 5 cut(s) 99, 713, 1271, 2050, 2186
SphI GCATGC 2 cut(s) 807, 2284
Sse8387I CCTGCAGG 1 cut(s) 856
SseBI AGGCCT 1 cut(s) 2074
SsiI CCGC 6 cut(s) 95, 348, 948, 1188, 1703, 1913
SspI AATATT 1 cut(s) 589
SspMI CTAG 4 cut(s) 74, 1472, 1505, 2102
SstI GAGCTC 1 cut(s) 714
StuI AGGCCT 1 cut(s) 2074
StyD4I CCNGG 8 cut(s) 248, 258, 456, 877, 973, 1395, 1923, 2134
StyI CCWWGG 3 cut(s) 6, 845, 2166
TaaI ACNGT 5 cut(s) 128, 523, 595, 1149, 1686
TaiI ACGT 4 cut(s) 257, 962, 1842, 1935
TaqI TCGA 9 cut(s) 207, 510, 695, 705, 1350, 1660, 1899, 1980, 2317
TatI WGTACW 1 cut(s) 1170
TauI GCSGC 1 cut(s) 1706
TfiI GAWTC 2 cut(s) 730, 1235
Tru1I TTAA 9 cut(s) 354, 516, 639, 744, 828, 939, 1272, 1524, 2051
Tru9I TTAA 9 cut(s) 354, 516, 639, 744, 828, 939, 1272, 1524, 2051
TscAI CASTG 5 cut(s) 131, 189, 448, 547, 2266
TseFI GTSAC 4 cut(s) 131, 268, 1835, 2190
TseI GCWGC 9 cut(s) 56, 59, 435, 446, 449, 648, 1244, 1468, 1598
Tsp45I GTSAC 4 cut(s) 131, 268, 1835, 2190
TspGWI ACGGA 3 cut(s) 256, 808, 2189
TspRI CASTG 5 cut(s) 131, 189, 448, 547, 2266
Vha464I CTTAAG 2 cut(s) 1271, 2050
VpaK11BI GGWCC 5 cut(s) 834, 1019, 1795, 1961, 2176
XagI CCTNNNNNAGG 1 cut(s) 293
XapI RAATTY 5 cut(s) 635, 814, 967, 1068, 1133
XceI RCATGY 4 cut(s) 499, 771, 807, 2284
XcmI CCANNNNNNNNNTGG 1 cut(s) 246
XmiI GTMKAC 1 cut(s) 1335
XmnI GAANNNNTTC 1 cut(s) 1806
XspI CTAG 4 cut(s) 74, 1472, 1505, 2102
ZrmI AGTACT 1 cut(s) 1172
Zsp2I ATGCAT 2 cut(s) 805, 1891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.