RchiOBHm_Chr1g0329511

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004224
Physical Location & Seq
Reverse (-)
19174308 .. 19177711
3404 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55882

Sequence Viewer

Length: 2328 bp
ATGGCCTTAGTAGTACATGGGAAAATGCTTCTTATGAAACGCTCATTAGTCTTATTAGCAGCTGCAACGACATTGCTAGCTGATAAAACCCCCTCGCCGCCTCAAGCCAAGCCCAACTGCCCAGACCGCTGTGGTAACCTGTCAATTCCATATCCATTTGGCATCGGTGAAGGCTGTTATTTACCACTGAAGGGAAAGAAAAAGCAATTCGAACTCGCTTGTAATTATTCTACTAAACCCCCATCATTGACCTGGCCGACGGACCGCTCCGCCAGTATCTCTGGTTTTCACCTTGCTGAGGGTGAGTTGGAAGTCATGAATTCGATAAGCAAGGATTGTTACAACAAGAGCGGTTTTAATGTAGAGGAATACTTCTCTCTCTCTTGGTCTCCTCCTTTCAGCCTTTCCGGTCGAAAAAACAGCTTCGTCGCCATAGGCTGCGACACTTCTGCTGCTTTCCTGGGTTTTCGTGGCCAAAAACAAATCATGACGGGGTGCATGTCCGTGTGCCTCGATATTAACACCGTGGATCTAAACTCTTGCACCGGTGTCGGTTGTTGCCAGACCAAGATCCCTGATGAACTGAATAATTTGACTGTGAGATTGGATAGCTATTTCAATCATTCGTATGTACGGGAATTCAATCCCTGCAGCTATGCCTTCGTTGTGAAAGATGGCTACTTTAACTTCAGCGGTACGAGGAGTTTCAAAGAGCTCAAGAACATGGAGCAGGTTCCACTTATCGGTAATTGGCAGATTGGAAATGAGACATGCGATGTAGCTAAAAAGAATGCCGTGAGTTATGCATGCAAGGCAAATTCCGTGTGCGTCAACCGGAGCAAGAGCCCATGGCCTGCAGGTTACTACTGCCAATGCTCACCAGGCTACGAAGGGAACCCATACATCGGTTGCCGAGATATTGACGAGTGTTTGGCTGAAATTAACCCATGCGGGAATGGAACGTGCAAGAATATACCTGGAAGTTACTCTTGTAAATGTAAAAAAGGGTTCAAAAGTGATGGTCCTTTCAAGTGCATTGCTCGTCCCACGGCAAACAACAGTGCTCTGAAAATTTCACTGGGTATTTGTATAGGCTTCTTTGTTCTATTAGTCGTGATTTTTGGATTATATCGCGAATACAAGAGAAGACAGTTCAACGAAATGAAAAAAAAGTATTTTGAAGCAAACGGCGGGCCAAAATTACAACTACAATTGAAGCAATTGGCTAGTCAAAAAGAACCTCTTGCAGTGCAAATCTTTACTGAAGAAGAACTTAAGAAGGCAACAAAGAATTACGATGAAAATGAAAAACTTGGAGAAGGAGGCTATGGAATAGTCTACAAAGGCGTACTCGATAAAAGAGTGGTTGCAATAAAGATATCCAAAATGACTGCCCAGGTTGAAAGTGATCAATTTATCAATGAGGTCATTGTTCTTTCTCAAATCAGTCATAGAAATGTGGTGAGGCTGCTAGGCTGTTGTTTGGAGACAAAAACGCCTATGCTAGTTTACGAGTATGTTGGTAGTGGAACTCTTTATGACCACATTCATAAAAAGAAAGGACAACTACTTTCATTTGCACAACGATTGAAGATAGCAGCAGAAACTGCGACAGCACTTTCATACTTACATCACTCCACCACCACGCAAATTGTTCATCGAGATGTCAAGGCAACGAATATACTGTTGGATGAGAAACTTATGGCCAAAGTGTCGGACTTCGGAGCTTCAAAACTTATTCCTGACGACAAAACTCAACTTTCAACTCTAGTGCAGGGGACATTGGGATACTTGGACCCTGAATATCTTCAGTCCAACACTCTGACAGAAAAGAGTGACGTTTATAGCTTTGGAGTCGTCCTCGTGGAGCTACTGACAAGCGAGAATGTAATTCGTTTTGATAAAACCGAGGCAGAGAGAAACCTGGCCAACGTCTTTGTTTCTACAATTGAAAAAAAAGGCCTGCGCGCATTGGGTCCAATTCTTGACGATGAAATAGTCAAGGATGGAAACTGGGAGATCATCGAAAAAGTTGCCAATCTTGCCCAAAGATGCTTAAGTGTGAAAGGAGACGAAAGGCCTACCATGAAAGAAATAGAGAGGGAGCTAGATGAAATACGGAAAACTTCCGCAAAGCAACCAGATGGGAAGAAGCTTTATGCCTCCTCTTCCAAAGAGACGGACCCCTCTCTTGAGTCACATTCAGACGCTTACAATGTGGAGATCAGTGATGAAGGCGATGGTGGTTCTACTGGCATTATTAGCAGTACAGAGTATGACGCCAGCATGCAAAATCAAGCCCAAAAGTCGTCCAGTTATGGTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK cEGF

Protein Analysis

775

Amino Acids

85.77

Weight (kDa)

8.18

Isoelectric Point (pI)

32.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 40 - 103 1.8e-08 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 306 - 345 5.2e-08 Calcium-binding EGF domain
Pkinase PF00069 432 - 699 2.2e-50 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 435 - 703 9.9e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 721, 848
AccBSI CCGCTC 2 cut(s) 267, 351
AccI GTMKAC 1 cut(s) 1338
AccII CGCG 2 cut(s) 1134, 1968
AciI CCGC 9 cut(s) 98, 127, 265, 270, 351, 693, 951, 1191, 2130
AclWI GGATC 2 cut(s) 537, 565
AcoI YGGCCR 4 cut(s) 254, 472, 1704, 1926
AcsI RAATTY 4 cut(s) 319, 638, 817, 1071
AcuI CTGAAG 4 cut(s) 209, 673, 1284, 1793
AcyI GRCGYC 1 cut(s) 2280
AfaI GTAC 5 cut(s) 15, 633, 697, 1350, 2269
AfiI CCNNNNNNNGG 4 cut(s) 191, 298, 743, 905
AflII CTTAAG 2 cut(s) 1274, 2056
AgeI ACCGGT 1 cut(s) 545
AjnI CCWGG 6 cut(s) 251, 459, 880, 976, 1395, 1923
AjuI GAANNNNNNNTTGG 2 cut(s) 1670, 1702
Alw21I GWGCWC 2 cut(s) 717, 1066
Alw26I GTCTC 5 cut(s) 393, 761, 1481, 2064, 2171
AlwI GGATC 2 cut(s) 537, 565
AlwNI CAGNNNCTG 1 cut(s) 1607
AoxI GGCC 9 cut(s) 3, 254, 472, 851, 1193, 1704, 1926, 1960, 2078
ApeKI GCWGC 7 cut(s) 59, 62, 438, 452, 651, 1468, 1598
ApoI RAATTY 4 cut(s) 319, 638, 817, 1071
AsiGI ACCGGT 1 cut(s) 545
Asp700I GAANNNNTTC 1 cut(s) 1806
AspLEI GCGC 2 cut(s) 1968, 1970
AspS9I GGNCC 6 cut(s) 262, 1022, 1193, 1795, 1976, 2182
AsuHPI GGTGA 5 cut(s) 179, 281, 314, 870, 1474
AsuII TTCGAA 1 cut(s) 210
AsuNHI GCTAGC 1 cut(s) 76
AvaII GGWCC 5 cut(s) 262, 1022, 1795, 1976, 2182
BalI TGGCCA 3 cut(s) 474, 1706, 1928
BanII GRGCYC 2 cut(s) 717, 848
BauI CACGAG 1 cut(s) 1862
BbsI GAAGAC 1 cut(s) 1153
Bbv12I GWGCWC 2 cut(s) 717, 1066
BbvCI CCTCAGC 1 cut(s) 297
BbvI GCAGC 7 cut(s) 49, 71, 425, 439, 663, 1455, 1610
BccI CCATC 6 cut(s) 250, 668, 1013, 2000, 2138, 2234
BceAI ACGGC 3 cut(s) 779, 1065, 1204
BcgI CGANNNNNNTGC 6 cut(s) 431, 465, 532, 566, 2015, 2049
BciT130I CCWGG 6 cut(s) 253, 461, 882, 978, 1397, 1925
BciVI GTATCC 1 cut(s) 1781
BclI TGATCA 1 cut(s) 1408
BcoDI GTCTC 5 cut(s) 393, 761, 1481, 2064, 2171
BfaI CTAG 6 cut(s) 77, 1227, 1472, 1505, 1769, 2108
BfmI CTRYAG 2 cut(s) 649, 855
BfrI CTTAAG 2 cut(s) 1274, 2056
BfuAI ACCTGC 2 cut(s) 721, 848
BfuI GTATCC 1 cut(s) 1781
BisI GCNGC 8 cut(s) 60, 63, 98, 439, 453, 652, 1469, 1599
BlsI GCNGC 8 cut(s) 61, 64, 99, 440, 454, 653, 1470, 1600
Bme1390I CCNGG 6 cut(s) 253, 461, 882, 978, 1397, 1925
Bme18I GGWCC 5 cut(s) 262, 1022, 1795, 1976, 2182
BmgT120I GGNCC 6 cut(s) 262, 1022, 1193, 1795, 1976, 2182
BmiI GGNNCC 5 cut(s) 735, 896, 1797, 1977, 2184
BmrFI CCNGG 6 cut(s) 253, 461, 882, 978, 1397, 1925
BmrI ACTGGG 2 cut(s) 1088, 2023
BmsI GCATC 2 cut(s) 171, 2042
BmtI GCTAGC 1 cut(s) 80
BmuI ACTGGG 2 cut(s) 1088, 2023
BpiI GAAGAC 1 cut(s) 1153
BplI GAGNNNNNCTC 2 cut(s) 1845, 1877
Bpu10I CCTNAGC 1 cut(s) 297
Bpu14I TTCGAA 1 cut(s) 210
BpuEI CTTGAG 3 cut(s) 87, 701, 2213
BsaHI GRCGYC 1 cut(s) 2280
BsaI GGTCTC 1 cut(s) 393
BsaJI CCNNGG 6 cut(s) 460, 525, 848, 1047, 1395, 1908
BsaWI WCCGGW 3 cut(s) 407, 545, 834
Bsc4I CCNNNNNNNGG 4 cut(s) 191, 298, 743, 905
Bse118I RCCGGY 1 cut(s) 545
Bse1I ACTGG 5 cut(s) 273, 1083, 2018, 2257, 2313
Bse3DI GCAATG 2 cut(s) 71, 1035
BseBI CCWGG 6 cut(s) 253, 461, 882, 978, 1397, 1925
BseDI CCNNGG 6 cut(s) 460, 525, 848, 1047, 1395, 1908
BseGI GGATG 2 cut(s) 1696, 2011
BseLI CCNNNNNNNGG 4 cut(s) 191, 298, 743, 905
BseMI GCAATG 2 cut(s) 71, 1035
BseMII CTCAG 1 cut(s) 288
BseNI ACTGG 5 cut(s) 273, 1083, 2018, 2257, 2313
BsePI GCGCGC 1 cut(s) 1966
BseRI GAGGAG 3 cut(s) 381, 715, 2155
BseXI GCAGC 7 cut(s) 49, 71, 425, 439, 663, 1455, 1610
BsgI GTGCAG 1 cut(s) 1793
Bsh1236I CGCG 2 cut(s) 1134, 1968
Bsh1285I CGRYCG 1 cut(s) 412
BshFI GGCC 9 cut(s) 5, 256, 474, 853, 1195, 1706, 1928, 1962, 2080
BshTI ACCGGT 1 cut(s) 545
BsiEI CGRYCG 1 cut(s) 412
BsiHKAI GWGCWC 2 cut(s) 717, 1066
BsiSI CCGG 3 cut(s) 408, 546, 835
BslFI GGGAC 2 cut(s) 1029, 1792
BslI CCNNNNNNNGG 4 cut(s) 191, 298, 743, 905
BsmAI GTCTC 5 cut(s) 393, 761, 1481, 2064, 2171
BsmBI CGTCTC 2 cut(s) 2064, 2171
BsmFI GGGAC 2 cut(s) 1029, 1792
BsmI GAATGC 1 cut(s) 796
BsnI GGCC 9 cut(s) 5, 256, 474, 853, 1195, 1706, 1928, 1962, 2080
Bso31I GGTCTC 1 cut(s) 393
Bsp119I TTCGAA 1 cut(s) 210
Bsp1286I GDGCHC 3 cut(s) 717, 848, 1066
Bsp143I GATC 5 cut(s) 529, 570, 1408, 2019, 2223
Bsp19I CCATGG 1 cut(s) 848
Bsp68I TCGCGA 1 cut(s) 1134
BspACI CCGC 9 cut(s) 98, 127, 265, 270, 351, 693, 951, 1191, 2130
BspANI GGCC 9 cut(s) 5, 256, 474, 853, 1195, 1706, 1928, 1962, 2080
BspCNI CTCAG 1 cut(s) 289
BspFNI CGCG 2 cut(s) 1134, 1968
BspHI TCATGA 2 cut(s) 315, 486
BspLI GGNNCC 5 cut(s) 735, 896, 1797, 1977, 2184
BspMAI CTGCAG 2 cut(s) 653, 859
BspMI ACCTGC 2 cut(s) 721, 848
BspOI GCTAGC 1 cut(s) 80
BspPI GGATC 2 cut(s) 537, 565
BspT104I TTCGAA 1 cut(s) 210
BspTI CTTAAG 2 cut(s) 1274, 2056
BspTNI GGTCTC 1 cut(s) 393
BsrBI CCGCTC 2 cut(s) 267, 351
BsrDI GCAATG 2 cut(s) 71, 1035
BsrFI RCCGGY 1 cut(s) 545
BsrI ACTGG 5 cut(s) 273, 1083, 2018, 2257, 2313
BssAI RCCGGY 1 cut(s) 545
BssECI CCNNGG 6 cut(s) 460, 525, 848, 1047, 1395, 1908
BssHII GCGCGC 1 cut(s) 1966
BssMI GATC 5 cut(s) 529, 570, 1408, 2019, 2223
BssNI GRCGYC 1 cut(s) 2280
BssSI CACGAG 1 cut(s) 1862
BssT1I CCWWGG 1 cut(s) 848
Bst2BI CACGAG 1 cut(s) 1862
Bst2UI CCWGG 6 cut(s) 253, 461, 882, 978, 1397, 1925
Bst4CI ACNGT 5 cut(s) 526, 598, 1061, 1152, 1686
Bst6I CTCTTC 1 cut(s) 2173
BstACI GRCGYC 1 cut(s) 2280
BstAFI CTTAAG 2 cut(s) 1274, 2056
BstAPI GCANNNNNTGC 1 cut(s) 1607
BstBI TTCGAA 1 cut(s) 210
BstC8I GCNNGC 8 cut(s) 78, 808, 855, 1193, 1964, 1968, 2284, 2288
BstDEI CTNAG 2 cut(s) 7, 297
BstDSI CCRYGG 3 cut(s) 525, 848, 1047
BstEII GGTNACC 1 cut(s) 134
BstENI CCTNNNNNAGG 1 cut(s) 296
BstF5I GGATG 2 cut(s) 1696, 2011
BstFNI CGCG 2 cut(s) 1134, 1968
BstHHI GCGC 2 cut(s) 1968, 1970
BstKTI GATC 5 cut(s) 532, 573, 1411, 2022, 2226
BstMAI GTCTC 5 cut(s) 393, 761, 1481, 2064, 2171
BstMBI GATC 5 cut(s) 529, 570, 1408, 2019, 2223
BstMCI CGRYCG 1 cut(s) 412
BstMWI GCNNNNNNNGC 6 cut(s) 126, 812, 882, 1607, 2042, 2262
BstNI CCWGG 6 cut(s) 253, 461, 882, 978, 1397, 1925
BstNSI RCATGY 4 cut(s) 502, 774, 810, 2290
BstPI GGTNACC 1 cut(s) 134
BstSCI CCNGG 6 cut(s) 251, 459, 880, 976, 1395, 1923
BstSFI CTRYAG 2 cut(s) 649, 855
BstUI CGCG 2 cut(s) 1134, 1968
BstV1I GCAGC 7 cut(s) 49, 71, 425, 439, 663, 1455, 1610
BstV2I GAAGAC 1 cut(s) 1153
BstX2I RGATCY 2 cut(s) 529, 570
BstYI RGATCY 2 cut(s) 529, 570
BsuI GTATCC 1 cut(s) 1781
BsuRI GGCC 9 cut(s) 5, 256, 474, 853, 1195, 1706, 1928, 1962, 2080
BtgI CCRYGG 3 cut(s) 525, 848, 1047
BtgZI GCGATG 2 cut(s) 789, 2253
BtsCI GGATG 2 cut(s) 1696, 2011
BtsI GCAGTG 1 cut(s) 1254
BtsIMutI CAGTG 5 cut(s) 185, 1066, 1076, 1254, 2233
BtuMI TCGCGA 1 cut(s) 1134
BveI ACCTGC 2 cut(s) 721, 848
Cac8I GCNNGC 8 cut(s) 78, 808, 855, 1193, 1964, 1968, 2284, 2288
CaiI CAGNNNCTG 1 cut(s) 1607
CciI TCATGA 2 cut(s) 315, 486
CfoI GCGC 2 cut(s) 1968, 1970
Cfr10I RCCGGY 1 cut(s) 545
Cfr13I GGNCC 6 cut(s) 262, 1022, 1193, 1795, 1976, 2182
CpoI CGGWCCG 1 cut(s) 262
CseI GACGC 3 cut(s) 817, 2216, 2288
Csp6I GTAC 5 cut(s) 14, 632, 696, 1349, 2268
CspAI ACCGGT 1 cut(s) 545
CspCI CAANNNNNGTGG 2 cut(s) 1633, 1668
CspI CGGWCCG 1 cut(s) 262
CviQI GTAC 5 cut(s) 14, 632, 696, 1349, 2268
DdeI CTNAG 2 cut(s) 7, 297
DpnI GATC 5 cut(s) 531, 572, 1410, 2021, 2225
DpnII GATC 5 cut(s) 529, 570, 1408, 2019, 2223
EaeI YGGCCR 4 cut(s) 254, 472, 1704, 1926
Eam1104I CTCTTC 1 cut(s) 2173
EarI CTCTTC 1 cut(s) 2173
EciI GGCGGA 1 cut(s) 259
Ecl136II GAGCTC 1 cut(s) 715
Eco130I CCWWGG 1 cut(s) 848
Eco147I AGGCCT 2 cut(s) 1962, 2080
Eco24I GRGCYC 2 cut(s) 717, 848
Eco31I GGTCTC 1 cut(s) 393
Eco32I GATATC 1 cut(s) 1380
Eco47I GGWCC 5 cut(s) 262, 1022, 1795, 1976, 2182
Eco53kI GAGCTC 1 cut(s) 715
Eco57I CTGAAG 4 cut(s) 209, 673, 1284, 1793
Eco91I GGTNACC 1 cut(s) 134
EcoICRI GAGCTC 1 cut(s) 715
EcoNI CCTNNNNNAGG 1 cut(s) 296
EcoO65I GGTNACC 1 cut(s) 134
EcoRI GAATTC 2 cut(s) 319, 638
EcoRII CCWGG 6 cut(s) 251, 459, 880, 976, 1395, 1923
EcoRV GATATC 1 cut(s) 1380
EcoT14I CCWWGG 1 cut(s) 848
EcoT22I ATGCAT 1 cut(s) 808
EcoT38I GRGCYC 2 cut(s) 717, 848
ErhI CCWWGG 1 cut(s) 848
Esp3I CGTCTC 2 cut(s) 2064, 2171
FalI AAGNNNNNCTT 6 cut(s) 973, 1005, 1227, 1259, 1257, 1289
FaqI GGGAC 2 cut(s) 1029, 1792
FauI CCCGC 2 cut(s) 944, 1184
FbaI TGATCA 1 cut(s) 1408
FblI GTMKAC 1 cut(s) 1338
Fnu4HI GCNGC 8 cut(s) 60, 63, 98, 439, 453, 652, 1469, 1599
FokI GGATG 2 cut(s) 1703, 2018
FriOI GRGCYC 2 cut(s) 717, 848
Fsp4HI GCNGC 8 cut(s) 60, 63, 98, 439, 453, 652, 1469, 1599
FspBI CTAG 6 cut(s) 77, 1227, 1472, 1505, 1769, 2108
GlaI GCGC 2 cut(s) 1967, 1969
GluI GCNGC 8 cut(s) 60, 63, 98, 439, 453, 652, 1469, 1599
HaeIII GGCC 9 cut(s) 5, 256, 474, 853, 1195, 1706, 1928, 1962, 2080
HapII CCGG 3 cut(s) 408, 546, 835
HgaI GACGC 3 cut(s) 817, 2216, 2288
HhaI GCGC 2 cut(s) 1968, 1970
Hin1I GRCGYC 1 cut(s) 2280
Hin6I GCGC 2 cut(s) 1966, 1968
HinP1I GCGC 2 cut(s) 1966, 1968
HincII GTYRAC 1 cut(s) 832
HindII GTYRAC 1 cut(s) 832
HindIII AAGCTT 1 cut(s) 2153
HinfI GANTC 2 cut(s) 1854, 2195
HpaII CCGG 3 cut(s) 408, 546, 835
HphI GGTGA 5 cut(s) 179, 281, 314, 870, 1474
Hpy166II GTNNAC 3 cut(s) 832, 1339, 1510
Hpy188I TCNGA 5 cut(s) 1068, 1717, 1724, 1824, 2206
Hpy188III TCNNGA 9 cut(s) 316, 487, 718, 1114, 1133, 1661, 1742, 1985, 2192
Hpy8I GTNNAC 3 cut(s) 832, 1339, 1510
Hpy99I CGWCG 2 cut(s) 262, 431
HpyAV CCTTC 7 cut(s) 164, 184, 670, 884, 1273, 1313, 2228
HpyCH4III ACNGT 5 cut(s) 526, 598, 1061, 1152, 1686
HpyCH4IV ACGT 3 cut(s) 962, 1839, 1932
HpyF10VI GCNNNNNNNGC 6 cut(s) 126, 812, 882, 1607, 2042, 2262
HpyF3I CTNAG 2 cut(s) 7, 297
HpySE526I ACGT 3 cut(s) 962, 1839, 1932
Hsp92I GRCGYC 1 cut(s) 2280
HspAI GCGC 2 cut(s) 1966, 1968
Ksp22I TGATCA 1 cut(s) 1408
Kzo9I GATC 5 cut(s) 529, 570, 1408, 2019, 2223
LmnI GCTCC 6 cut(s) 272, 727, 837, 1724, 1867, 2104
Lsp1109I GCAGC 7 cut(s) 49, 71, 425, 439, 663, 1455, 1610
LweI GCATC 2 cut(s) 171, 2042
MaeI CTAG 6 cut(s) 77, 1227, 1472, 1505, 1769, 2108
MaeII ACGT 3 cut(s) 962, 1839, 1932
MaeIII GTNAC 6 cut(s) 134, 338, 860, 983, 1835, 2196
MalI GATC 5 cut(s) 531, 572, 1410, 2021, 2225
MbiI CCGCTC 2 cut(s) 267, 351
MboI GATC 5 cut(s) 529, 570, 1408, 2019, 2223
MboII GAAGA 7 cut(s) 1158, 1277, 1280, 1603, 1799, 2160, 2161
MfeI CAATTG 3 cut(s) 1211, 1220, 1947
MflI RGATCY 2 cut(s) 529, 570
MhlI GDGCHC 3 cut(s) 717, 848, 1066
MlsI TGGCCA 3 cut(s) 474, 1706, 1928
MluNI TGGCCA 3 cut(s) 474, 1706, 1928
MlyI GAGTC 2 cut(s) 1863, 2204
MmeI TCCRAC 4 cut(s) 288, 1668, 1695, 1839
Mox20I TGGCCA 3 cut(s) 474, 1706, 1928
Mph1103I ATGCAT 1 cut(s) 808
MroXI GAANNNNTTC 1 cut(s) 1806
MscI TGGCCA 3 cut(s) 474, 1706, 1928
MseI TTAA 6 cut(s) 357, 519, 684, 942, 1275, 2057
MslI CAYNNNNRTG 4 cut(s) 503, 627, 1455, 1662
Msp20I TGGCCA 3 cut(s) 474, 1706, 1928
MspA1I CMGCKG 3 cut(s) 62, 129, 693
MspCI CTTAAG 2 cut(s) 1274, 2056
MspI CCGG 3 cut(s) 408, 546, 835
MspR9I CCNGG 6 cut(s) 253, 461, 882, 978, 1397, 1925
MunI CAATTG 3 cut(s) 1211, 1220, 1947
Mva1269I GAATGC 1 cut(s) 796
MvaI CCWGG 6 cut(s) 253, 461, 882, 978, 1397, 1925
MvnI CGCG 2 cut(s) 1134, 1968
MwoI GCNNNNNNNGC 6 cut(s) 126, 812, 882, 1607, 2042, 2262
NcoI CCATGG 1 cut(s) 848
NdeII GATC 5 cut(s) 529, 570, 1408, 2019, 2223
NheI GCTAGC 1 cut(s) 76
NlaIV GGNNCC 5 cut(s) 735, 896, 1797, 1977, 2184
NmeAIII GCCGAG 1 cut(s) 938
NmuCI GTSAC 2 cut(s) 1835, 2196
NruI TCGCGA 1 cut(s) 1134
NsiI ATGCAT 1 cut(s) 808
NspI RCATGY 4 cut(s) 502, 774, 810, 2290
NspV TTCGAA 1 cut(s) 210
PaeI GCATGC 2 cut(s) 810, 2290
PagI TCATGA 2 cut(s) 315, 486
PauI GCGCGC 1 cut(s) 1966
PceI AGGCCT 2 cut(s) 1962, 2080
PctI GAATGC 1 cut(s) 796
PdmI GAANNNNTTC 1 cut(s) 1806
PinAI ACCGGT 1 cut(s) 545
PkrI GCNGC 8 cut(s) 61, 64, 99, 440, 454, 653, 1470, 1600
PleI GAGTC 2 cut(s) 1862, 2203
PpsI GAGTC 2 cut(s) 1862, 2203
Psp124BI GAGCTC 1 cut(s) 717
Psp6I CCWGG 6 cut(s) 251, 459, 880, 976, 1395, 1923
PspEI GGTNACC 1 cut(s) 134
PspGI CCWGG 6 cut(s) 251, 459, 880, 976, 1395, 1923
PspN4I GGNNCC 5 cut(s) 735, 896, 1797, 1977, 2184
PspPI GGNCC 6 cut(s) 262, 1022, 1193, 1795, 1976, 2182
PstI CTGCAG 2 cut(s) 653, 859
PstNI CAGNNNCTG 1 cut(s) 1607
PsuI RGATCY 2 cut(s) 529, 570
PteI GCGCGC 1 cut(s) 1966
PvuII CAGCTG 1 cut(s) 62
RruI TCGCGA 1 cut(s) 1134
RsaI GTAC 5 cut(s) 15, 633, 697, 1350, 2269
RsaNI GTAC 5 cut(s) 14, 632, 696, 1349, 2268
RseI CAYNNNNRTG 4 cut(s) 503, 627, 1455, 1662
Rsr2I CGGWCCG 1 cut(s) 262
RsrII CGGWCCG 1 cut(s) 262
SacI GAGCTC 1 cut(s) 717
SaqAI TTAA 6 cut(s) 357, 519, 684, 942, 1275, 2057
SatI GCNGC 8 cut(s) 60, 63, 98, 439, 453, 652, 1469, 1599
Sau3AI GATC 5 cut(s) 529, 570, 1408, 2019, 2223
Sau96I GGNCC 6 cut(s) 262, 1022, 1193, 1795, 1976, 2182
SbfI CCTGCAGG 1 cut(s) 859
SchI GAGTC 2 cut(s) 1863, 2204
ScrFI CCNGG 6 cut(s) 253, 461, 882, 978, 1397, 1925
SdaI CCTGCAGG 1 cut(s) 859
SduI GDGCHC 3 cut(s) 717, 848, 1066
SfaNI GCATC 2 cut(s) 171, 2042
SfcI CTRYAG 2 cut(s) 649, 855
SfuI TTCGAA 1 cut(s) 210
SgrAI CRCCGGYG 1 cut(s) 545
SinI GGWCC 5 cut(s) 262, 1022, 1795, 1976, 2182
SmiMI CAYNNNNRTG 4 cut(s) 503, 627, 1455, 1662
SmlI CTYRAG 5 cut(s) 102, 716, 1274, 2056, 2192
SmoI CTYRAG 5 cut(s) 102, 716, 1274, 2056, 2192
SphI GCATGC 2 cut(s) 810, 2290
Sse8387I CCTGCAGG 1 cut(s) 859
SseBI AGGCCT 2 cut(s) 1962, 2080
SsiI CCGC 9 cut(s) 98, 127, 265, 270, 351, 693, 951, 1191, 2130
SspMI CTAG 6 cut(s) 77, 1227, 1472, 1505, 1769, 2108
SstI GAGCTC 1 cut(s) 717
StuI AGGCCT 2 cut(s) 1962, 2080
StyD4I CCNGG 6 cut(s) 251, 459, 880, 976, 1395, 1923
StyI CCWWGG 1 cut(s) 848
TaaI ACNGT 5 cut(s) 526, 598, 1061, 1152, 1686
TaiI ACGT 3 cut(s) 965, 1842, 1935
TaqI TCGA 8 cut(s) 210, 323, 412, 513, 1353, 1660, 2025, 2323
TatI WGTACW 2 cut(s) 13, 2267
TauI GCSGC 1 cut(s) 100
Tru1I TTAA 6 cut(s) 357, 519, 684, 942, 1275, 2057
Tru9I TTAA 6 cut(s) 357, 519, 684, 942, 1275, 2057
TscAI CASTG 5 cut(s) 192, 1066, 1083, 1254, 2233
TseFI GTSAC 2 cut(s) 1835, 2196
TseI GCWGC 7 cut(s) 59, 62, 438, 452, 651, 1468, 1598
Tsp45I GTSAC 2 cut(s) 1835, 2196
TspGWI ACGGA 5 cut(s) 275, 493, 811, 2134, 2195
TspRI CASTG 5 cut(s) 192, 1066, 1083, 1254, 2233
Vha464I CTTAAG 2 cut(s) 1274, 2056
VpaK11BI GGWCC 5 cut(s) 262, 1022, 1795, 1976, 2182
XagI CCTNNNNNAGG 1 cut(s) 296
XapI RAATTY 4 cut(s) 319, 638, 817, 1071
XceI RCATGY 4 cut(s) 502, 774, 810, 2290
XcmI CCANNNNNNNNNTGG 1 cut(s) 249
XmiI GTMKAC 1 cut(s) 1338
XmnI GAANNNNTTC 1 cut(s) 1806
XspI CTAG 6 cut(s) 77, 1227, 1472, 1505, 1769, 2108
Zsp2I ATGCAT 1 cut(s) 808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.