RLG00000023080

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0004224
Physical Location & Seq
Reverse (-)
17787366 .. 17809899
22534 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023080

Sequence Viewer

Length: 2280 bp
ATGCATCTTATGAAACTCACATTAGTCTTATTAGCAGCTGCAACGACATTGCTAGCTGCAGCAGCTGATAAACCCCTCCCACCGCCTCAAGCCAAGCCCAACTGTCAAGACCGCTGTGGTGACCTCACAATTCCATATCCATTTGGCATCGGTGACGGCTGTTACATGCTACTGAAGGGACAGGAAACGACATTCGAACTCACCTGTGATCATTCCACTAATCCCCCATCATTGAACTTGCCCCCCAGCATCCGTGTCACCGGTATTAATCTTAATGAGGGTGAGTTGGAAGCTATGAATTTTATAAGCAAGGATTGTTACAACAAGAGCGGATCTAATGAGGAGAACAACGTTCCTGGGCTCTGGTGGGCTCCTCCTTTCAGTATTTCCGGTTCCGAAAACAGGTTCTTCGCCGTAGGCTGCGACACTTACGCTGTTTTCCAGGGTTTTCGTGGCGAAGAAGAGTTCAGGTTAGGGTGCATGTCCGTGTGCCTCAACATCAGCAAAGTGGATCAAAACTCTTGCTCTGGTGTCGGGTGTTGCCAGATCAAAATCCCTGAAGGACTGAAGAATCTTACTGTAACATTGAGTAGCTATTACAATCATACGGATATATGGAGATTTAATCCCTGCAGCTATGCCTTCCTTGTAAAAGATGGCTACTTTAACTTCAGCGGTGAGAGGAGTTTCGAAGAGCTCAAGAACACAGATGAAACGCCGATGATCGTTAATTGGCAGATTGGAAATGAGACATGCGAGGTAGCTGAAAAGAATGCTGTGGATTATGCATGCAAGTCAAATTCCAAATGTGTCAACCAGATTAAGGGCCCATACCCTGGTTATCATTGCCAGTGCTTAGACGGCTACGAAGGGAACCCATACATAGGTTGCCGAGATATTGACGAGTGTTTGGCTGAAATTAAGCCATGCGCAAATGGAATGTGCCAAAATTCACCTGGAACCTACTCTTGTAAATGTAATAAAGGGTTCAAAAATGATGGCCTTTTGAAGTGCATTGCTAGTCCCACGGCAAACAACACTCCTCTGAAAGTTTCATTGGGTATTTGTATAGGCTGCTTTGTTCTATTAGTCGTAGTTTTTGGGCTATATCGCGGGTGCAAGAGAAGACAGTTCAACAAAATGAAAGAAAAGTACTTTCACACAAATGGCGGGCCAAAATTAGAACTGCTATTAAAACAATTGGCTAGTGAAAATGAACCTCTTCCAGCCAAAATCTTTACTGCAGAAGAACTTAAGAAAGCAACAAAGGATTACGACGAAAATGAAAAAATTGGTGAAGGAGGTTATGGAACAGTCTACAAAGGCGTACTCGAAGATAAAAGAGTGGTTGCAATAAAGATGTCCAAAATGACTACCCAGGTCGGAAGCGATCAATTTATTAACGAGGTCATTGTTCTTTCTCAAATCCGTCATAGAAATGTGGTAAGGCTGCTAGGCTGTTGTTTGGACACAAAAACGCCTTTGCTAGTTTACGAGTATGTTGGTAATGGAACTCTGTATGATCACATTCATAAAAAGAAAGGAGAACCACTTTCATTTGCACAGCGATTGAAGATAGCAGCAGAAACTGCGAGGGCACTTTCGCACTTACATCGTTCTGCCATCCATCGAATTATTCATCGAGATGTCAAGGCAACGAATATACTGTTGGATGAGAAACTTACGGCCAAAGTGACGGACTTCGGAGCTTCAAAACTGGTTCCTGACGATAAAACTATACTTTCAACTTTAGTGCAGGGGACAATGGGATACTTGGACCCTGAATATCTTCGGTCCAACACACTAACAGAAAAGAGTGACGTTTATAGCTTTGGAGTTGTCCTCGTGGAGCTACTGACAGGTGAAAATGCAGTTCGTTTCGATAAACCCGAGGAGGAGAGAAACCTCGCCAACGTCTTTGTTTCTACAATAAATAAAGGTCCGCTGCATCCAATTCTTGATGGTGAAATAGTCAAGGATGGAAATTGGGAGATCATCGAAAGAGTTGCCAAGCTCGCAGAAGAATGCTTAAGTGTGAAAGGAGATGAAAGACCTCCCATGGAAGTGGTAGAGAGGGAGCTAGATGAAATACTGAAAACTATGGAGAAGCAACCAGGGGGAAAGAAGCTTCCCTTCTTTCCAGATCTTCCATCGATGCTTCTTTATGAATACTTTGTAAAACAACCAGCATGTGAGAATGGAGATCATGTGAATGATGCTGCTCAAGCTTACAACGTGGTACTGGGAATTGCCATCCCAATGCATAGAAAGGACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK cEGF

Protein Analysis

760

Amino Acids

84.4

Weight (kDa)

5.94

Isoelectric Point (pI)

33.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 35 - 97 5.3e-11 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 299 - 334 9.2e-08 Calcium-binding EGF domain
Pkinase PF00069 425 - 688 1.1e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 428 - 693 3e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 305
Acc16I TGCGCA 1 cut(s) 931
AccB7I CCANNNNNTGG 1 cut(s) 836
AccBSI CCGCTC 1 cut(s) 330
AccI GTMKAC 1 cut(s) 1317
AccII CGCG 1 cut(s) 1113
AciI CCGC 7 cut(s) 83, 112, 330, 675, 1113, 1170, 1943
AclI AACGTT 1 cut(s) 351
AclWI GGATC 2 cut(s) 340, 519
AcoI YGGCCR 1 cut(s) 1686
AcsI RAATTY 3 cut(s) 298, 799, 949
AcuI CTGAAG 4 cut(s) 194, 579, 587, 655
AfaI GTAC 3 cut(s) 1154, 1329, 2241
AfiI CCNNNNNNNGG 4 cut(s) 402, 823, 836, 884
AflII CTTAAG 2 cut(s) 1253, 2029
AgeI ACCGGT 1 cut(s) 260
AgsI TTSAA 7 cut(s) 235, 991, 1009, 1135, 1573, 1713, 1746
AjnI CCWGG 6 cut(s) 355, 441, 835, 955, 1377, 2113
AjuI GAANNNNNNNTTGG 4 cut(s) 1040, 1072, 1652, 1684
Alw21I GWGCWC 1 cut(s) 699
Alw26I GTCTC 1 cut(s) 743
AlwI GGATC 2 cut(s) 340, 519
AlwNI CAGNNNCTG 2 cut(s) 65, 1589
Ama87I CYCGRG 1 cut(s) 1889
AoxI GGCC 4 cut(s) 826, 1000, 1172, 1686
ApaI GGGCCC 1 cut(s) 830
ApoI RAATTY 3 cut(s) 298, 799, 949
AseI ATTAAT 1 cut(s) 267
AsiGI ACCGGT 1 cut(s) 260
Asp700I GAANNNNTTC 2 cut(s) 1221, 1788
AspLEI GCGC 1 cut(s) 932
AspS9I GGNCC 6 cut(s) 826, 827, 1172, 1777, 1794, 1940
AsuII TTCGAA 2 cut(s) 195, 690
AsuNHI GCTAGC 1 cut(s) 52
AvaI CYCGRG 1 cut(s) 1889
AvaII GGWCC 3 cut(s) 1777, 1794, 1940
BaeGI GKGCMC 2 cut(s) 830, 1600
BanII GRGCYC 4 cut(s) 363, 373, 699, 830
BauI CACGAG 1 cut(s) 1844
BbsI GAAGAC 1 cut(s) 1132
Bbv12I GWGCWC 1 cut(s) 699
BccI CCATC 9 cut(s) 235, 650, 992, 1631, 1635, 1955, 1973, 2158, 2261
BceAI ACGGC 5 cut(s) 172, 398, 877, 1044, 1701
BcgI CGANNNNNNTGC 4 cut(s) 1595, 1629, 1988, 2022
BciT130I CCWGG 6 cut(s) 357, 443, 837, 957, 1379, 2115
BciVI GTATCC 1 cut(s) 1763
BclI TGATCA 2 cut(s) 208, 1522
BcoDI GTCTC 1 cut(s) 743
BfaI CTAG 6 cut(s) 53, 1020, 1206, 1454, 1487, 2081
BfmI CTRYAG 3 cut(s) 57, 631, 1242
BfrI CTTAAG 2 cut(s) 1253, 2029
BfuI GTATCC 1 cut(s) 1763
BglII AGATCT 1 cut(s) 2143
BmcAI AGTACT 1 cut(s) 1154
Bme1390I CCNGG 6 cut(s) 357, 443, 837, 957, 1379, 2115
Bme18I GGWCC 3 cut(s) 1777, 1794, 1940
BmeT110I CYCGRG 1 cut(s) 1889
BmgT120I GGNCC 6 cut(s) 826, 827, 1172, 1777, 1794, 1940
BmiI GGNNCC 7 cut(s) 372, 394, 828, 875, 961, 1722, 1779
BmrFI CCNGG 6 cut(s) 357, 443, 837, 957, 1379, 2115
BmrI ACTGGG 1 cut(s) 2252
BmsI GCATC 6 cut(s) 13, 156, 258, 1957, 2145, 2206
BmtI GCTAGC 1 cut(s) 56
BmuI ACTGGG 1 cut(s) 2252
BpiI GAAGAC 1 cut(s) 1132
BplI GAGNNNNNCTC 2 cut(s) 1827, 1859
Bpu14I TTCGAA 2 cut(s) 195, 690
BpuEI CTTGAG 3 cut(s) 72, 683, 2208
Bsa29I ATCGAT 1 cut(s) 2153
BsaBI GATNNNNATC 1 cut(s) 551
BsaJI CCNNGG 8 cut(s) 356, 442, 835, 1026, 1377, 1890, 2058, 2114
BsaWI WCCGGW 2 cut(s) 260, 389
BsaXI ACNNNNNCTCC 2 cut(s) 2193, 2223
Bsc4I CCNNNNNNNGG 4 cut(s) 402, 823, 836, 884
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 3 cut(s) 850, 1722, 2247
Bse3DI GCAATG 3 cut(s) 47, 844, 1014
Bse8I GATNNNNATC 1 cut(s) 551
BseBI CCWGG 6 cut(s) 357, 443, 837, 957, 1379, 2115
BseCI ATCGAT 1 cut(s) 2153
BseDI CCNNGG 8 cut(s) 356, 442, 835, 1026, 1377, 1890, 2058, 2114
BseGI GGATG 6 cut(s) 249, 1623, 1678, 1948, 1984, 2253
BseJI GATNNNNATC 1 cut(s) 551
BseLI CCNNNNNNNGG 4 cut(s) 402, 823, 836, 884
BseMI GCAATG 3 cut(s) 47, 844, 1014
BseNI ACTGG 3 cut(s) 850, 1722, 2247
BseRI GAGGAG 6 cut(s) 356, 363, 697, 1032, 1907, 1910
BseSI GKGCMC 2 cut(s) 830, 1600
BseYI CCCAGC 1 cut(s) 245
BsgI GTGCAG 1 cut(s) 1775
Bsh1236I CGCG 1 cut(s) 1113
BshFI GGCC 4 cut(s) 828, 1002, 1174, 1688
BshTI ACCGGT 1 cut(s) 260
BshVI ATCGAT 1 cut(s) 2153
BsiHKAI GWGCWC 1 cut(s) 699
BsiHKCI CYCGRG 1 cut(s) 1889
BsiSI CCGG 2 cut(s) 261, 390
BslFI GGGAC 3 cut(s) 192, 1008, 1774
BslI CCNNNNNNNGG 4 cut(s) 402, 823, 836, 884
BsmAI GTCTC 1 cut(s) 743
BsmFI GGGAC 3 cut(s) 192, 1008, 1774
BsmI GAATGC 2 cut(s) 778, 2030
BsnI GGCC 4 cut(s) 828, 1002, 1174, 1688
BsoBI CYCGRG 1 cut(s) 1889
Bsp119I TTCGAA 2 cut(s) 195, 690
Bsp120I GGGCCC 1 cut(s) 826
Bsp1286I GDGCHC 5 cut(s) 363, 373, 699, 830, 1600
Bsp19I CCATGG 1 cut(s) 2058
BspACI CCGC 7 cut(s) 83, 112, 330, 675, 1113, 1170, 1943
BspANI GGCC 4 cut(s) 828, 1002, 1174, 1688
BspDI ATCGAT 1 cut(s) 2153
BspFNI CGCG 1 cut(s) 1113
BspLI GGNNCC 7 cut(s) 372, 394, 828, 875, 961, 1722, 1779
BspMAI CTGCAG 3 cut(s) 61, 635, 1246
BspOI GCTAGC 1 cut(s) 56
BspPI GGATC 2 cut(s) 340, 519
BspQI GCTCTTC 1 cut(s) 687
BspT104I TTCGAA 2 cut(s) 195, 690
BspTI CTTAAG 2 cut(s) 1253, 2029
BsrBI CCGCTC 1 cut(s) 330
BsrDI GCAATG 3 cut(s) 47, 844, 1014
BsrFI RCCGGY 1 cut(s) 260
BsrI ACTGG 3 cut(s) 850, 1722, 2247
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 8 cut(s) 356, 442, 835, 1026, 1377, 1890, 2058, 2114
BssSI CACGAG 1 cut(s) 1844
BssT1I CCWWGG 1 cut(s) 2058
Bst2BI CACGAG 1 cut(s) 1844
Bst2UI CCWGG 6 cut(s) 357, 443, 837, 957, 1379, 2115
Bst4CI ACNGT 5 cut(s) 104, 580, 1131, 1315, 1668
Bst6I CTCTTC 3 cut(s) 456, 687, 1227
BstAFI CTTAAG 2 cut(s) 1253, 2029
BstAPI GCANNNNNTGC 1 cut(s) 1589
BstBI TTCGAA 2 cut(s) 195, 690
BstC8I GCNNGC 4 cut(s) 54, 790, 1172, 2016
BstDEI CTNAG 1 cut(s) 856
BstDSI CCRYGG 2 cut(s) 1026, 2058
BstEII GGTNACC 1 cut(s) 119
BstF5I GGATG 6 cut(s) 249, 1623, 1678, 1948, 1984, 2253
BstFNI CGCG 1 cut(s) 1113
BstHHI GCGC 1 cut(s) 932
BstMAI GTCTC 1 cut(s) 743
BstMWI GCNNNNNNNGC 5 cut(s) 62, 861, 1589, 2015, 2225
BstNI CCWGG 6 cut(s) 357, 443, 837, 957, 1379, 2115
BstNSI RCATGY 5 cut(s) 169, 484, 756, 792, 2193
BstPI GGTNACC 1 cut(s) 119
BstSCI CCNGG 6 cut(s) 355, 441, 835, 955, 1377, 2113
BstSFI CTRYAG 3 cut(s) 57, 631, 1242
BstSLI GKGCMC 2 cut(s) 830, 1600
BstUI CGCG 1 cut(s) 1113
BstV2I GAAGAC 1 cut(s) 1132
BstX2I RGATCY 2 cut(s) 332, 2143
BstXI CCANNNNNNTGG 1 cut(s) 2065
BstYI RGATCY 2 cut(s) 332, 2143
Bsu15I ATCGAT 1 cut(s) 2153
BsuI GTATCC 1 cut(s) 1763
BsuRI GGCC 4 cut(s) 828, 1002, 1174, 1688
BsuTUI ATCGAT 1 cut(s) 2153
BtgI CCRYGG 2 cut(s) 1026, 2058
BtsCI GGATG 6 cut(s) 249, 1623, 1678, 1948, 1984, 2253
BtsIMutI CAGTG 1 cut(s) 857
Cac8I GCNNGC 4 cut(s) 54, 790, 1172, 2016
CaiI CAGNNNCTG 2 cut(s) 65, 1589
CfoI GCGC 1 cut(s) 932
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 6 cut(s) 826, 827, 1172, 1777, 1794, 1940
ClaI ATCGAT 1 cut(s) 2153
Csp6I GTAC 3 cut(s) 1153, 1328, 2240
CspAI ACCGGT 1 cut(s) 260
CviAII CATG 8 cut(s) 166, 481, 753, 789, 927, 2059, 2190, 2207
CviQI GTAC 3 cut(s) 1153, 1328, 2240
DdeI CTNAG 1 cut(s) 856
EaeI YGGCCR 1 cut(s) 1686
Eam1104I CTCTTC 3 cut(s) 456, 687, 1227
EarI CTCTTC 3 cut(s) 456, 687, 1227
Ecl136II GAGCTC 1 cut(s) 697
Eco130I CCWWGG 1 cut(s) 2058
Eco24I GRGCYC 4 cut(s) 363, 373, 699, 830
Eco47I GGWCC 3 cut(s) 1777, 1794, 1940
Eco53kI GAGCTC 1 cut(s) 697
Eco57I CTGAAG 4 cut(s) 194, 579, 587, 655
Eco88I CYCGRG 1 cut(s) 1889
Eco91I GGTNACC 1 cut(s) 119
EcoICRI GAGCTC 1 cut(s) 697
EcoO109I RGGNCCY 1 cut(s) 826
EcoO65I GGTNACC 1 cut(s) 119
EcoRII CCWGG 6 cut(s) 355, 441, 835, 955, 1377, 2113
EcoT14I CCWWGG 1 cut(s) 2058
EcoT22I ATGCAT 3 cut(s) 6, 790, 2265
EcoT38I GRGCYC 4 cut(s) 363, 373, 699, 830
ErhI CCWWGG 1 cut(s) 2058
FaeI CATG 8 cut(s) 169, 484, 756, 792, 930, 2062, 2193, 2210
FalI AAGNNNNNCTT 2 cut(s) 2117, 2149
FaqI GGGAC 3 cut(s) 192, 1008, 1774
FatI CATG 8 cut(s) 165, 480, 752, 788, 926, 2058, 2189, 2206
FauI CCCGC 2 cut(s) 1106, 1163
FbaI TGATCA 2 cut(s) 208, 1522
FblI GTMKAC 1 cut(s) 1317
FokI GGATG 6 cut(s) 236, 1610, 1685, 1935, 1991, 2240
FriOI GRGCYC 4 cut(s) 363, 373, 699, 830
FspBI CTAG 6 cut(s) 53, 1020, 1206, 1454, 1487, 2081
FspI TGCGCA 1 cut(s) 931
GlaI GCGC 1 cut(s) 931
GsaI CCCAGC 1 cut(s) 249
HaeIII GGCC 4 cut(s) 828, 1002, 1174, 1688
HapII CCGG 2 cut(s) 261, 390
HhaI GCGC 1 cut(s) 932
Hin1II CATG 8 cut(s) 169, 484, 756, 792, 930, 2062, 2193, 2210
Hin6I GCGC 1 cut(s) 930
HinP1I GCGC 1 cut(s) 930
HincII GTYRAC 1 cut(s) 814
HindII GTYRAC 1 cut(s) 814
HindIII AAGCTT 2 cut(s) 2126, 2226
HinfI GANTC 1 cut(s) 571
HpaII CCGG 2 cut(s) 261, 390
Hpy166II GTNNAC 3 cut(s) 814, 1318, 1492
Hpy188I TCNGA 4 cut(s) 397, 1047, 1385, 1706
Hpy188III TCNNGA 6 cut(s) 107, 700, 1643, 1724, 1958, 2141
Hpy8I GTNNAC 3 cut(s) 814, 1318, 1492
Hpy99I CGWCG 1 cut(s) 1280
HpyAV CCTTC 6 cut(s) 169, 554, 652, 863, 1292, 2143
HpyCH4III ACNGT 5 cut(s) 104, 580, 1131, 1315, 1668
HpyCH4IV ACGT 4 cut(s) 351, 1821, 1914, 2235
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 861, 1589, 2015, 2225
HpyF3I CTNAG 1 cut(s) 856
HpySE526I ACGT 4 cut(s) 351, 1821, 1914, 2235
Hsp92II CATG 8 cut(s) 169, 484, 756, 792, 930, 2062, 2193, 2210
HspAI GCGC 1 cut(s) 930
Ksp22I TGATCA 2 cut(s) 208, 1522
LguI GCTCTTC 1 cut(s) 687
LmnI GCTCC 4 cut(s) 376, 1706, 1849, 2077
LweI GCATC 6 cut(s) 13, 156, 258, 1957, 2145, 2206
MaeI CTAG 6 cut(s) 53, 1020, 1206, 1454, 1487, 2081
MaeII ACGT 4 cut(s) 351, 1821, 1914, 2235
MaeIII GTNAC 8 cut(s) 119, 152, 161, 256, 317, 580, 1693, 1817
MbiI CCGCTC 1 cut(s) 330
MfeI CAATTG 1 cut(s) 1199
MflI RGATCY 2 cut(s) 332, 2143
MhlI GDGCHC 5 cut(s) 363, 373, 699, 830, 1600
MmeI TCCRAC 4 cut(s) 267, 1363, 1650, 1821
Mph1103I ATGCAT 3 cut(s) 6, 790, 2265
MroXI GAANNNNTTC 2 cut(s) 1221, 1788
MslI CAYNNNNRTG 7 cut(s) 485, 1164, 1437, 1644, 2063, 2211, 2258
MspA1I CMGCKG 5 cut(s) 38, 65, 114, 675, 1945
MspCI CTTAAG 2 cut(s) 1253, 2029
MspI CCGG 2 cut(s) 261, 390
MspR9I CCNGG 6 cut(s) 357, 443, 837, 957, 1379, 2115
MunI CAATTG 1 cut(s) 1199
Mva1269I GAATGC 2 cut(s) 778, 2030
MvaI CCWGG 6 cut(s) 357, 443, 837, 957, 1379, 2115
MvnI CGCG 1 cut(s) 1113
MwoI GCNNNNNNNGC 5 cut(s) 62, 861, 1589, 2015, 2225
NcoI CCATGG 1 cut(s) 2058
NheI GCTAGC 1 cut(s) 52
NlaIII CATG 8 cut(s) 169, 484, 756, 792, 930, 2062, 2193, 2210
NlaIV GGNNCC 7 cut(s) 372, 394, 828, 875, 961, 1722, 1779
NmeAIII GCCGAG 1 cut(s) 917
NmuCI GTSAC 5 cut(s) 119, 152, 256, 1693, 1817
NsbI TGCGCA 1 cut(s) 931
NsiI ATGCAT 3 cut(s) 6, 790, 2265
NspI RCATGY 5 cut(s) 169, 484, 756, 792, 2193
NspV TTCGAA 2 cut(s) 195, 690
PaeI GCATGC 1 cut(s) 792
PciSI GCTCTTC 1 cut(s) 687
PcsI WCGNNNNNNNCGW 1 cut(s) 1887
PctI GAATGC 2 cut(s) 778, 2030
PdmI GAANNNNTTC 2 cut(s) 1221, 1788
PfeI GAWTC 1 cut(s) 571
PflMI CCANNNNNTGG 1 cut(s) 836
PinAI ACCGGT 1 cut(s) 260
PshBI ATTAAT 1 cut(s) 267
PsiI TTATAA 1 cut(s) 305
Psp124BI GAGCTC 1 cut(s) 699
Psp1406I AACGTT 1 cut(s) 351
Psp6I CCWGG 6 cut(s) 355, 441, 835, 955, 1377, 2113
PspEI GGTNACC 1 cut(s) 119
PspFI CCCAGC 1 cut(s) 245
PspGI CCWGG 6 cut(s) 355, 441, 835, 955, 1377, 2113
PspN4I GGNNCC 7 cut(s) 372, 394, 828, 875, 961, 1722, 1779
PspOMI GGGCCC 1 cut(s) 826
PspPI GGNCC 6 cut(s) 826, 827, 1172, 1777, 1794, 1940
PsrI GAACNNNNNNTAC 2 cut(s) 376, 408
PstI CTGCAG 3 cut(s) 61, 635, 1246
PstNI CAGNNNCTG 2 cut(s) 65, 1589
PsuI RGATCY 2 cut(s) 332, 2143
PvuII CAGCTG 2 cut(s) 38, 65
RsaI GTAC 3 cut(s) 1154, 1329, 2241
RsaNI GTAC 3 cut(s) 1153, 1328, 2240
RseI CAYNNNNRTG 7 cut(s) 485, 1164, 1437, 1644, 2063, 2211, 2258
SacI GAGCTC 1 cut(s) 699
SapI GCTCTTC 1 cut(s) 687
Sau96I GGNCC 6 cut(s) 826, 827, 1172, 1777, 1794, 1940
ScaI AGTACT 1 cut(s) 1154
ScrFI CCNGG 6 cut(s) 357, 443, 837, 957, 1379, 2115
SduI GDGCHC 5 cut(s) 363, 373, 699, 830, 1600
SfaNI GCATC 6 cut(s) 13, 156, 258, 1957, 2145, 2206
SfcI CTRYAG 3 cut(s) 57, 631, 1242
SfuI TTCGAA 2 cut(s) 195, 690
SinI GGWCC 3 cut(s) 1777, 1794, 1940
SmiMI CAYNNNNRTG 7 cut(s) 485, 1164, 1437, 1644, 2063, 2211, 2258
SmlI CTYRAG 5 cut(s) 87, 698, 1253, 2029, 2223
SmoI CTYRAG 5 cut(s) 87, 698, 1253, 2029, 2223
SphI GCATGC 1 cut(s) 792
SsiI CCGC 7 cut(s) 83, 112, 330, 675, 1113, 1170, 1943
SspMI CTAG 6 cut(s) 53, 1020, 1206, 1454, 1487, 2081
SstI GAGCTC 1 cut(s) 699
StyD4I CCNGG 6 cut(s) 355, 441, 835, 955, 1377, 2113
StyI CCWWGG 1 cut(s) 2058
TaaI ACNGT 5 cut(s) 104, 580, 1131, 1315, 1668
TaiI ACGT 4 cut(s) 354, 1824, 1917, 2238
TaqI TCGA 8 cut(s) 195, 690, 1332, 1630, 1642, 1881, 1998, 2153
TaqII GACCGA 1 cut(s) 1782
TatI WGTACW 1 cut(s) 1152
TfiI GAWTC 1 cut(s) 571
TscAI CASTG 1 cut(s) 857
TseFI GTSAC 5 cut(s) 119, 152, 256, 1693, 1817
Tsp45I GTSAC 5 cut(s) 119, 152, 256, 1693, 1817
TspGWI ACGGA 5 cut(s) 242, 475, 623, 1418, 1712
TspRI CASTG 1 cut(s) 857
Van91I CCANNNNNTGG 1 cut(s) 836
Vha464I CTTAAG 2 cut(s) 1253, 2029
VpaK11BI GGWCC 3 cut(s) 1777, 1794, 1940
VspI ATTAAT 1 cut(s) 267
XapI RAATTY 3 cut(s) 298, 799, 949
XceI RCATGY 5 cut(s) 169, 484, 756, 792, 2193
XcmI CCANNNNNNNNNTGG 2 cut(s) 449, 953
XmiI GTMKAC 1 cut(s) 1317
XmnI GAANNNNTTC 2 cut(s) 1221, 1788
XspI CTAG 6 cut(s) 53, 1020, 1206, 1454, 1487, 2081
ZrmI AGTACT 1 cut(s) 1154
Zsp2I ATGCAT 3 cut(s) 6, 790, 2265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.