Rw3G024480

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0004224
Physical Location & Seq
Forward (+)
31515265 .. 31517964
2700 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G024480.1

Sequence Viewer

Length: 1227 bp
ATGAAAAAAAAGTACTTTGAAGCAAACGGCGGGCCAAAATTAGAACTACAGTTAAAGCAATTGGCTAGTCAAAAAGAATCTCTTACAGCCCAAATCTTTACTGCTGAAGAACTTAAGAAGGCAACAAAGGATTACGATGAAAATGAAAAAATTGGTGAAGGAGGTTATGGAACAATCTACAAAGGCGTACTCGAAGATAAAAGAGTGGTTGCAATAAAAAAGTCCAAATTGACTGCCCAGGTCAGAAATGATCAATTTATTAATGAGATCATTGTTCTTTCTCAAATCAGTCATAAAAATGTGGTGAGGCTGCTAGGCTGTTGTTTGGAGACAAAAATGCCTTTGCTAGTTTACGAGTATGTTAGTAATGGAACTCTGTATGATCACATTCATAAAAAGAATGGAGAACCACTTTCATTTGCACAACGATTGAAGATAGCAACAGAAACTGCGAGGGCACTTTCATACTTACATCTTTCCGCCATCCATCGAATTATTCATCGAGATATCAAGGCAACGAATATACTGTTGGATGAGAAACTTACGGCCAAAGTGACGGACTTCGGAGCTTCAAAACTGGTTCCTGACGATAAAACTATACTTTCAACTATAGTGCAGGGGACAGTGGGATACTTGGACCCTGAATATCTTCGGTCCCACACACTAACAGAAAAGAGTGACGTTTATAGCTTTGGAGTTGTCCTCGTGGAGCTACTGACAGGCGAAAATGCATTTCGTTTTGAAAAACCCGAGGCAGAGAGAAACCTGGCCAACGTCTTTGTTTCTACAATAAAGAACGGCCCGCTGCATCCAATTCTTGATGGTGAAATAGTCAAGGATGGAAATTGGGAGATCATCGAAAGAGTTGCCAAGCTTGCAGAAGAATGCTTAAGTGTGAAAGGAGATGAAAGACCTCCCATGGAAGTGGTAGAGAGGAAGCTAGATGAAATACTGAAAACTTGGGAAAATCAACCAAGGGAAAAGAAGCTTCGTGCCTCCTCTTCCAAAGAGACGGACCACTCTCTCGAGTCACTTTCAAACACTTGCATTGTGGAGGTTAGTGGTGAAGACGATGGTGGCTCTACCGGGATTATTAGCAGTGCAGAGTATGACGCCAGCTTGCAGAATCAAGCCCAAAAGCCCTTCCATGATGAATTTCAATCAAGCAATCTTTCTCTATTGAAGAAACAAAGAAACTTGCTGGGCATCTCTCTGCGTCAGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK cEGF

Protein Analysis

408

Amino Acids

45.78

Weight (kDa)

6.26

Isoelectric Point (pI)

30.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 45 - 308 1.5e-51 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 48 - 313 3.9e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 30, 480, 803
AcoI YGGCCR 2 cut(s) 546, 768
AcsI RAATTY 1 cut(s) 1154
AcuI CTGAAG 1 cut(s) 126
AcyI GRCGYC 1 cut(s) 1113
AfaI GTAC 2 cut(s) 14, 189
AflII CTTAAG 2 cut(s) 113, 889
AgsI TTSAA 8 cut(s) 20, 433, 573, 606, 743, 1038, 1160, 1183
AjnI CCWGG 2 cut(s) 237, 765
AjuI GAANNNNNNNTTGG 2 cut(s) 512, 544
AluBI AGCT 7 cut(s) 569, 690, 712, 874, 940, 988, 1119
AluI AGCT 7 cut(s) 569, 690, 712, 874, 940, 988, 1119
Alw26I GTCTC 2 cut(s) 323, 1004
AlwNI CAGNNNCTG 1 cut(s) 449
Ama87I CYCGRG 2 cut(s) 749, 1025
AoxI GGCC 4 cut(s) 32, 546, 768, 799
ApeKI GCWGC 2 cut(s) 310, 805
ApoI RAATTY 1 cut(s) 1154
AseI ATTAAT 1 cut(s) 261
Asp700I GAANNNNTTC 1 cut(s) 648
AspS9I GGNCC 5 cut(s) 32, 637, 654, 800, 1015
AsuC2I CCSGG 1 cut(s) 1087
AsuHPI GGTGA 4 cut(s) 167, 316, 836, 1076
AvaI CYCGRG 2 cut(s) 749, 1025
AvaII GGWCC 3 cut(s) 637, 654, 1015
BaeGI GKGCMC 1 cut(s) 460
BalI TGGCCA 1 cut(s) 770
BauI CACGAG 1 cut(s) 704
BbsI GAAGAC 1 cut(s) 1074
BbvI GCAGC 2 cut(s) 297, 792
BccI CCATC 5 cut(s) 491, 495, 815, 833, 1067
BceAI ACGGC 3 cut(s) 43, 561, 814
BcgI CGANNNNNNTGC 2 cut(s) 848, 882
BciT130I CCWGG 2 cut(s) 239, 767
BciVI GTATCC 1 cut(s) 623
BclI TGATCA 2 cut(s) 250, 382
BcnI CCSGG 1 cut(s) 1087
BcoDI GTCTC 2 cut(s) 323, 1004
BfaI CTAG 5 cut(s) 66, 314, 347, 941, 1225
BfmI CTRYAG 2 cut(s) 47, 609
BfrI CTTAAG 2 cut(s) 113, 889
BfuI GTATCC 1 cut(s) 623
BisI GCNGC 2 cut(s) 311, 806
BlsI GCNGC 2 cut(s) 312, 807
BmcAI AGTACT 1 cut(s) 14
Bme1390I CCNGG 3 cut(s) 239, 767, 1087
Bme18I GGWCC 3 cut(s) 637, 654, 1015
BmeT110I CYCGRG 2 cut(s) 749, 1025
BmgT120I GGNCC 5 cut(s) 32, 637, 654, 800, 1015
BmiI GGNNCC 3 cut(s) 582, 639, 656
BmrFI CCNGG 3 cut(s) 239, 767, 1087
BmsI GCATC 2 cut(s) 817, 1215
BpiI GAAGAC 1 cut(s) 1074
BplI GAGNNNNNCTC 2 cut(s) 687, 719
BpuMI CCSGG 1 cut(s) 1087
BsaHI GRCGYC 1 cut(s) 1113
BsaJI CCNNGG 4 cut(s) 237, 750, 918, 974
Bse1I ACTGG 1 cut(s) 582
BseBI CCWGG 2 cut(s) 239, 767
BseDI CCNNGG 4 cut(s) 237, 750, 918, 974
BseGI GGATG 4 cut(s) 483, 538, 808, 844
BseNI ACTGG 1 cut(s) 582
BseRI GAGGAG 1 cut(s) 988
BseSI GKGCMC 1 cut(s) 460
BseXI GCAGC 2 cut(s) 297, 792
BseYI CCCAGC 1 cut(s) 1201
BsgI GTGCAG 2 cut(s) 635, 1122
BshFI GGCC 4 cut(s) 34, 548, 770, 801
BsiHKCI CYCGRG 2 cut(s) 749, 1025
BsiSI CCGG 1 cut(s) 1086
BslFI GGGAC 2 cut(s) 634, 640
BsmAI GTCTC 2 cut(s) 323, 1004
BsmBI CGTCTC 1 cut(s) 1004
BsmFI GGGAC 2 cut(s) 634, 640
BsmI GAATGC 1 cut(s) 890
BsnI GGCC 4 cut(s) 34, 548, 770, 801
BsoBI CYCGRG 2 cut(s) 749, 1025
Bsp1286I GDGCHC 1 cut(s) 460
Bsp143I GATC 4 cut(s) 250, 267, 382, 852
Bsp19I CCATGG 1 cut(s) 918
BspACI CCGC 3 cut(s) 30, 480, 803
BspANI GGCC 4 cut(s) 34, 548, 770, 801
BspLI GGNNCC 3 cut(s) 582, 639, 656
BspTI CTTAAG 2 cut(s) 113, 889
BsrI ACTGG 1 cut(s) 582
BssECI CCNNGG 4 cut(s) 237, 750, 918, 974
BssMI GATC 4 cut(s) 250, 267, 382, 852
BssNI GRCGYC 1 cut(s) 1113
BssSI CACGAG 1 cut(s) 704
BssT1I CCWWGG 2 cut(s) 918, 974
Bst2BI CACGAG 1 cut(s) 704
Bst2UI CCWGG 2 cut(s) 239, 767
Bst4CI ACNGT 3 cut(s) 51, 528, 625
Bst6I CTCTTC 1 cut(s) 1006
BstACI GRCGYC 1 cut(s) 1113
BstAFI CTTAAG 2 cut(s) 113, 889
BstC8I GCNNGC 5 cut(s) 32, 803, 876, 1117, 1121
BstDSI CCRYGG 1 cut(s) 918
BstF5I GGATG 4 cut(s) 483, 538, 808, 844
BstKTI GATC 4 cut(s) 253, 270, 385, 855
BstMAI GTCTC 2 cut(s) 323, 1004
BstMBI GATC 4 cut(s) 250, 267, 382, 852
BstMWI GCNNNNNNNGC 1 cut(s) 875
BstNI CCWGG 2 cut(s) 239, 767
BstSCI CCNGG 3 cut(s) 237, 765, 1085
BstSFI CTRYAG 2 cut(s) 47, 609
BstSLI GKGCMC 1 cut(s) 460
BstV1I GCAGC 2 cut(s) 297, 792
BstV2I GAAGAC 1 cut(s) 1074
BstXI CCANNNNNNTGG 1 cut(s) 925
BsuI GTATCC 1 cut(s) 623
BsuRI GGCC 4 cut(s) 34, 548, 770, 801
BtgI CCRYGG 1 cut(s) 918
BtsCI GGATG 4 cut(s) 483, 538, 808, 844
BtsI GCAGTG 1 cut(s) 1105
BtsIMutI CAGTG 2 cut(s) 630, 1105
Cac8I GCNNGC 5 cut(s) 32, 803, 876, 1117, 1121
CaiI CAGNNNCTG 1 cut(s) 449
Cfr13I GGNCC 5 cut(s) 32, 637, 654, 800, 1015
CseI GACGC 2 cut(s) 1121, 1205
Csp6I GTAC 2 cut(s) 13, 188
CviAII CATG 2 cut(s) 919, 1148
CviQI GTAC 2 cut(s) 13, 188
DpnI GATC 4 cut(s) 252, 269, 384, 854
DpnII GATC 4 cut(s) 250, 267, 382, 852
EaeI YGGCCR 2 cut(s) 546, 768
Eam1104I CTCTTC 1 cut(s) 1006
EarI CTCTTC 1 cut(s) 1006
EciI GGCGGA 1 cut(s) 469
Eco130I CCWWGG 2 cut(s) 918, 974
Eco32I GATATC 1 cut(s) 508
Eco47I GGWCC 3 cut(s) 637, 654, 1015
Eco57I CTGAAG 1 cut(s) 126
Eco88I CYCGRG 2 cut(s) 749, 1025
EcoRII CCWGG 2 cut(s) 237, 765
EcoRV GATATC 1 cut(s) 508
EcoT14I CCWWGG 2 cut(s) 918, 974
EcoT22I ATGCAT 1 cut(s) 733
ErhI CCWWGG 2 cut(s) 918, 974
Esp3I CGTCTC 1 cut(s) 1004
FaeI CATG 2 cut(s) 922, 1151
FalI AAGNNNNNCTT 2 cut(s) 66, 98
FaqI GGGAC 2 cut(s) 634, 640
FatI CATG 2 cut(s) 918, 1147
FauI CCCGC 2 cut(s) 23, 810
FbaI TGATCA 2 cut(s) 250, 382
Fnu4HI GCNGC 2 cut(s) 311, 806
FokI GGATG 4 cut(s) 470, 545, 795, 851
Fsp4HI GCNGC 2 cut(s) 311, 806
FspBI CTAG 5 cut(s) 66, 314, 347, 941, 1225
GluI GCNGC 2 cut(s) 311, 806
GsaI CCCAGC 1 cut(s) 1205
HaeIII GGCC 4 cut(s) 34, 548, 770, 801
HapII CCGG 1 cut(s) 1086
HgaI GACGC 2 cut(s) 1121, 1205
Hin1I GRCGYC 1 cut(s) 1113
Hin1II CATG 2 cut(s) 922, 1151
HindIII AAGCTT 2 cut(s) 872, 986
HinfI GANTC 3 cut(s) 77, 1028, 1126
HpaII CCGG 1 cut(s) 1086
HphI GGTGA 4 cut(s) 167, 316, 836, 1076
Hpy166II GTNNAC 1 cut(s) 352
Hpy188I TCNGA 2 cut(s) 245, 566
Hpy188III TCNNGA 4 cut(s) 503, 584, 818, 1025
Hpy8I GTNNAC 1 cut(s) 352
HpyAV CCTTC 3 cut(s) 112, 152, 1153
HpyCH4III ACNGT 3 cut(s) 51, 528, 625
HpyCH4IV ACGT 2 cut(s) 681, 774
HpyCH4V TGCA 9 cut(s) 212, 422, 616, 731, 808, 878, 1047, 1103, 1123
HpyF10VI GCNNNNNNNGC 1 cut(s) 875
HpySE526I ACGT 2 cut(s) 681, 774
Hsp92I GRCGYC 1 cut(s) 1113
Hsp92II CATG 2 cut(s) 922, 1151
Ksp22I TGATCA 2 cut(s) 250, 382
Kzo9I GATC 4 cut(s) 250, 267, 382, 852
LmnI GCTCC 2 cut(s) 566, 709
Lsp1109I GCAGC 2 cut(s) 297, 792
LweI GCATC 2 cut(s) 817, 1215
MaeI CTAG 5 cut(s) 66, 314, 347, 941, 1225
MaeII ACGT 2 cut(s) 681, 774
MaeIII GTNAC 3 cut(s) 553, 677, 1029
MalI GATC 4 cut(s) 252, 269, 384, 854
MboI GATC 4 cut(s) 250, 267, 382, 852
MboII GAAGA 8 cut(s) 119, 206, 445, 641, 893, 993, 1079, 1195
MfeI CAATTG 1 cut(s) 59
MhlI GDGCHC 1 cut(s) 460
MlsI TGGCCA 1 cut(s) 770
MluCI AATT 9 cut(s) 38, 59, 150, 227, 254, 492, 813, 844, 1154
MluNI TGGCCA 1 cut(s) 770
MlyI GAGTC 1 cut(s) 1037
MmeI TCCRAC 1 cut(s) 510
Mox20I TGGCCA 1 cut(s) 770
Mph1103I ATGCAT 1 cut(s) 733
MroXI GAANNNNTTC 1 cut(s) 648
MscI TGGCCA 1 cut(s) 770
MseI TTAA 4 cut(s) 53, 114, 261, 890
MslI CAYNNNNRTG 2 cut(s) 297, 923
Msp20I TGGCCA 1 cut(s) 770
MspA1I CMGCKG 1 cut(s) 805
MspCI CTTAAG 2 cut(s) 113, 889
MspI CCGG 1 cut(s) 1086
MspR9I CCNGG 3 cut(s) 239, 767, 1087
MunI CAATTG 1 cut(s) 59
Mva1269I GAATGC 1 cut(s) 890
MvaI CCWGG 2 cut(s) 239, 767
MwoI GCNNNNNNNGC 1 cut(s) 875
NciI CCSGG 1 cut(s) 1087
NcoI CCATGG 1 cut(s) 918
NdeII GATC 4 cut(s) 250, 267, 382, 852
NlaIII CATG 2 cut(s) 922, 1151
NlaIV GGNNCC 3 cut(s) 582, 639, 656
NmuCI GTSAC 3 cut(s) 553, 677, 1029
NsiI ATGCAT 1 cut(s) 733
PaeR7I CTCGAG 1 cut(s) 1025
PctI GAATGC 1 cut(s) 890
PdmI GAANNNNTTC 1 cut(s) 648
PfeI GAWTC 2 cut(s) 77, 1126
PkrI GCNGC 2 cut(s) 312, 807
PleI GAGTC 1 cut(s) 1036
PpsI GAGTC 1 cut(s) 1036
PshBI ATTAAT 1 cut(s) 261
Psp6I CCWGG 2 cut(s) 237, 765
PspFI CCCAGC 1 cut(s) 1201
PspGI CCWGG 2 cut(s) 237, 765
PspN4I GGNNCC 3 cut(s) 582, 639, 656
PspPI GGNCC 5 cut(s) 32, 637, 654, 800, 1015
PstNI CAGNNNCTG 1 cut(s) 449
RsaI GTAC 2 cut(s) 14, 189
RsaNI GTAC 2 cut(s) 13, 188
RseI CAYNNNNRTG 2 cut(s) 297, 923
SaqAI TTAA 4 cut(s) 53, 114, 261, 890
SatI GCNGC 2 cut(s) 311, 806
Sau3AI GATC 4 cut(s) 250, 267, 382, 852
Sau96I GGNCC 5 cut(s) 32, 637, 654, 800, 1015
ScaI AGTACT 1 cut(s) 14
SchI GAGTC 1 cut(s) 1037
ScrFI CCNGG 3 cut(s) 239, 767, 1087
SduI GDGCHC 1 cut(s) 460
SfaNI GCATC 2 cut(s) 817, 1215
SfcI CTRYAG 2 cut(s) 47, 609
Sfr274I CTCGAG 1 cut(s) 1025
SinI GGWCC 3 cut(s) 637, 654, 1015
SlaI CTCGAG 1 cut(s) 1025
SmiMI CAYNNNNRTG 2 cut(s) 297, 923
SmlI CTYRAG 3 cut(s) 113, 889, 1025
SmoI CTYRAG 3 cut(s) 113, 889, 1025
Sse9I AATT 9 cut(s) 38, 59, 150, 227, 254, 492, 813, 844, 1154
SsiI CCGC 3 cut(s) 30, 480, 803
SspMI CTAG 5 cut(s) 66, 314, 347, 941, 1225
StyD4I CCNGG 3 cut(s) 237, 765, 1085
StyI CCWWGG 2 cut(s) 918, 974
TaaI ACNGT 3 cut(s) 51, 528, 625
TaiI ACGT 2 cut(s) 684, 777
TaqI TCGA 5 cut(s) 192, 490, 502, 858, 1026
TaqII GACCGA 1 cut(s) 642
TasI AATT 9 cut(s) 38, 59, 150, 227, 254, 492, 813, 844, 1154
TatI WGTACW 1 cut(s) 12
TfiI GAWTC 2 cut(s) 77, 1126
Tru1I TTAA 4 cut(s) 53, 114, 261, 890
Tru9I TTAA 4 cut(s) 53, 114, 261, 890
TscAI CASTG 2 cut(s) 630, 1105
TseFI GTSAC 3 cut(s) 553, 677, 1029
TseI GCWGC 2 cut(s) 310, 805
Tsp45I GTSAC 3 cut(s) 553, 677, 1029
TspGWI ACGGA 2 cut(s) 572, 1028
TspRI CASTG 2 cut(s) 630, 1105
Vha464I CTTAAG 2 cut(s) 113, 889
VpaK11BI GGWCC 3 cut(s) 637, 654, 1015
VspI ATTAAT 1 cut(s) 261
XapI RAATTY 1 cut(s) 1154
XhoI CTCGAG 1 cut(s) 1025
XmnI GAANNNNTTC 1 cut(s) 648
XspI CTAG 5 cut(s) 66, 314, 347, 941, 1225
ZrmI AGTACT 1 cut(s) 14
Zsp2I ATGCAT 1 cut(s) 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.