FvH4_3g11080

Protein phosphatase 2C 55

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004533
Physical Location & Seq
Forward (+)
6532064 .. 6532981
918 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g11080.t1

Sequence Viewer

Length: 918 bp
ATGGAAGGAGGAGGAGAGCAACCTTGTCAAGCTGAAGACTTGAATACGTCGTTCCCAAATAAGACGCTTATGGTTGAGGACATCAATCGTGTTGACTTGGGTAACAATGAACCCACTGGATCAAAAGAAAGAACCATTATGAAAATGGTTTGCGGGTCATTTTATTTGTCGAAAGAACAAAGAGACCATCCGATTGAGGGCGACGATGCTCACTTCATATGTGCAGAAAAGAACATCATTGGTGTGGCTGATGGCTTGGGTGGTACTCGAAAGTATGGCATTGATTCCGGAGTTTTCGCAAGGAAGCTCATGAATAATGCAGCCAAGGCCGTACATGATCAACATAGAATAATATCCCCAGATAGTAATGGTGTTGATCTGAGAAAGGCCTTGGATGAGGCTTATGCAAACACCAAGGATGTTGATGGGTCATCAACAGCTTGTATTGTTGCGCTTAATAACAAAGATGGAAAACTGCATGCTTTGAACGTTGGGGATAGCGGTTTCATGTTGTTTAGAAACAAGAAGTGCATCTTTAAGTCTGAAATACAGGTGCATCGTTTTAACTGTCCTGTTAAGTTGGGAAGAAATCAAGCAAAGCATGGTCCCCAATTTGCTGTGGAGATAGAACCGATTGATGTCATTCCGGGAGATGTTATAGTTCTTGGGACAGATGGGTTGCTTGACAATGTGTGGCCGGAAGAAATAGAGCAAGTTTTGGAGGTGAATATTATAGAAGGTTTTGTAGAACCGGAGCAGTTGGCTTGGACTATAGCTGAGCAGCTGGCTTTGCGGAACTCCGAGGATGTAAATAGGTTTAGTCCTTATTCCTTTGAATCTTGCAAGGTTGGGAAGGAGAGCATAGGAGGCAAGGTCGATGATATTACGGTCATAATAGGTCATATTATCGCAACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoIIE

Protein Analysis

306

Amino Acids

33.44

Weight (kDa)

4.99

Isoelectric Point (pI)

41.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoIIE PF07228 93 - 264 2e-07 Stage II sporulation protein E (SpoIIE)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 287
AciI CCGC 3 cut(s) 153, 501, 793
AclI AACGTT 1 cut(s) 489
AclWI GGATC 1 cut(s) 127
AcoI YGGCCR 1 cut(s) 695
AcuI CTGAAG 1 cut(s) 54
AfaI GTAC 2 cut(s) 265, 333
AfiI CCNNNNNNNGG 1 cut(s) 197
AgsI TTSAA 3 cut(s) 43, 487, 836
AluBI AGCT 5 cut(s) 32, 307, 440, 776, 784
AluI AGCT 5 cut(s) 32, 307, 440, 776, 784
Alw26I GTCTC 1 cut(s) 177
AlwI GGATC 1 cut(s) 127
Aor13HI TCCGGA 1 cut(s) 287
AoxI GGCC 3 cut(s) 327, 387, 695
ApeKI GCWGC 2 cut(s) 320, 781
AspLEI GCGC 1 cut(s) 454
AspS9I GGNCC 1 cut(s) 605
AsuC2I CCSGG 1 cut(s) 648
AsuHPI GGTGA 1 cut(s) 736
AvaII GGWCC 1 cut(s) 605
BbsI GAAGAC 1 cut(s) 42
BbvI GCAGC 2 cut(s) 332, 793
BccI CCATC 5 cut(s) 195, 245, 419, 461, 668
BceAI ACGGC 1 cut(s) 314
BclI TGATCA 1 cut(s) 337
BcnI CCSGG 1 cut(s) 648
BcoDI GTCTC 1 cut(s) 177
BfmI CTRYAG 1 cut(s) 771
BisI GCNGC 2 cut(s) 321, 782
BlpI GCTNAGC 1 cut(s) 777
BlsI GCNGC 2 cut(s) 322, 783
Bme1390I CCNGG 1 cut(s) 648
Bme18I GGWCC 1 cut(s) 605
BmgT120I GGNCC 1 cut(s) 605
BmiI GGNNCC 1 cut(s) 607
BmrFI CCNGG 1 cut(s) 648
BmsI GCATC 3 cut(s) 196, 540, 565
BpiI GAAGAC 1 cut(s) 42
Bpu1102I GCTNAGC 1 cut(s) 777
BpuMI CCSGG 1 cut(s) 648
BsaI GGTCTC 1 cut(s) 177
BsaJI CCNNGG 4 cut(s) 324, 390, 414, 801
BsaWI WCCGGW 2 cut(s) 287, 751
BsaXI ACNNNNNCTCC 2 cut(s) 858, 888
Bsc4I CCNNNNNNNGG 1 cut(s) 197
Bse1I ACTGG 1 cut(s) 121
BseAI TCCGGA 1 cut(s) 287
BseDI CCNNGG 4 cut(s) 324, 390, 414, 801
BseGI GGATG 4 cut(s) 187, 400, 424, 811
BseLI CCNNNNNNNGG 1 cut(s) 197
BseMII CTCAG 2 cut(s) 371, 768
BseNI ACTGG 1 cut(s) 121
BseRI GAGGAG 2 cut(s) 24, 27
BseXI GCAGC 2 cut(s) 332, 793
BsgI GTGCAG 1 cut(s) 243
BshFI GGCC 3 cut(s) 329, 389, 697
BsiSI CCGG 4 cut(s) 288, 647, 698, 752
BslFI GGGAC 2 cut(s) 591, 682
BslI CCNNNNNNNGG 1 cut(s) 197
BsmAI GTCTC 1 cut(s) 177
BsmFI GGGAC 2 cut(s) 591, 682
BsnI GGCC 3 cut(s) 329, 389, 697
Bso31I GGTCTC 1 cut(s) 177
Bsp13I TCCGGA 1 cut(s) 287
Bsp143I GATC 3 cut(s) 119, 337, 376
Bsp1720I GCTNAGC 1 cut(s) 777
BspACI CCGC 3 cut(s) 153, 501, 793
BspANI GGCC 3 cut(s) 329, 389, 697
BspCNI CTCAG 2 cut(s) 372, 769
BspEI TCCGGA 1 cut(s) 287
BspHI TCATGA 1 cut(s) 309
BspLI GGNNCC 1 cut(s) 607
BspPI GGATC 1 cut(s) 127
BspTNI GGTCTC 1 cut(s) 177
BsrI ACTGG 1 cut(s) 121
BssECI CCNNGG 4 cut(s) 324, 390, 414, 801
BssMI GATC 3 cut(s) 119, 337, 376
BssT1I CCWWGG 3 cut(s) 324, 390, 414
Bst4CI ACNGT 2 cut(s) 569, 889
BstC8I GCNNGC 2 cut(s) 480, 786
BstDEI CTNAG 2 cut(s) 380, 777
BstF5I GGATG 4 cut(s) 187, 400, 424, 811
BstHHI GCGC 1 cut(s) 454
BstKTI GATC 3 cut(s) 122, 340, 379
BstMAI GTCTC 1 cut(s) 177
BstMBI GATC 3 cut(s) 119, 337, 376
BstMWI GCNNNNNNNGC 3 cut(s) 326, 790, 867
BstNSI RCATGY 1 cut(s) 482
BstSCI CCNGG 1 cut(s) 646
BstSFI CTRYAG 1 cut(s) 771
BstV1I GCAGC 2 cut(s) 332, 793
BstV2I GAAGAC 1 cut(s) 42
BsuRI GGCC 3 cut(s) 329, 389, 697
BtsCI GGATG 4 cut(s) 187, 400, 424, 811
BtsIMutI CAGTG 1 cut(s) 114
Cac8I GCNNGC 2 cut(s) 480, 786
CciI TCATGA 1 cut(s) 309
CfoI GCGC 1 cut(s) 454
Cfr13I GGNCC 1 cut(s) 605
CseI GACGC 1 cut(s) 73
Csp6I GTAC 2 cut(s) 264, 332
CviAII CATG 5 cut(s) 310, 335, 479, 508, 602
CviQI GTAC 2 cut(s) 264, 332
DdeI CTNAG 2 cut(s) 380, 777
DpnI GATC 3 cut(s) 121, 339, 378
DpnII GATC 3 cut(s) 119, 337, 376
EaeI YGGCCR 1 cut(s) 695
Eco130I CCWWGG 3 cut(s) 324, 390, 414
Eco147I AGGCCT 1 cut(s) 389
Eco31I GGTCTC 1 cut(s) 177
Eco47I GGWCC 1 cut(s) 605
Eco57I CTGAAG 1 cut(s) 54
EcoT14I CCWWGG 3 cut(s) 324, 390, 414
ErhI CCWWGG 3 cut(s) 324, 390, 414
FaeI CATG 5 cut(s) 313, 338, 482, 511, 605
FalI AAGNNNNNCTT 2 cut(s) 518, 550
FaqI GGGAC 2 cut(s) 591, 682
FatI CATG 5 cut(s) 309, 334, 478, 507, 601
FauI CCCGC 1 cut(s) 146
FauNDI CATATG 1 cut(s) 218
FbaI TGATCA 1 cut(s) 337
Fnu4HI GCNGC 2 cut(s) 321, 782
FokI GGATG 4 cut(s) 174, 407, 431, 818
Fsp4HI GCNGC 2 cut(s) 321, 782
GlaI GCGC 1 cut(s) 453
GluI GCNGC 2 cut(s) 321, 782
HaeIII GGCC 3 cut(s) 329, 389, 697
HapII CCGG 4 cut(s) 288, 647, 698, 752
HgaI GACGC 1 cut(s) 73
HhaI GCGC 1 cut(s) 454
Hin1II CATG 5 cut(s) 313, 338, 482, 511, 605
Hin6I GCGC 1 cut(s) 452
HinP1I GCGC 1 cut(s) 452
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HinfI GANTC 2 cut(s) 284, 836
HpaII CCGG 4 cut(s) 288, 647, 698, 752
HphI GGTGA 1 cut(s) 736
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 4 cut(s) 192, 381, 544, 802
Hpy188III TCNNGA 2 cut(s) 288, 310
Hpy8I GTNNAC 1 cut(s) 94
Hpy99I CGWCG 2 cut(s) 52, 206
HpyAV CCTTC 2 cut(s) 731, 847
HpyCH4III ACNGT 2 cut(s) 569, 889
HpyCH4IV ACGT 3 cut(s) 47, 489, 914
HpyCH4V TGCA 7 cut(s) 224, 320, 407, 478, 531, 556, 843
HpyF10VI GCNNNNNNNGC 3 cut(s) 326, 790, 867
HpyF3I CTNAG 2 cut(s) 380, 777
HpySE526I ACGT 3 cut(s) 47, 489, 914
Hsp92II CATG 5 cut(s) 313, 338, 482, 511, 605
HspAI GCGC 1 cut(s) 452
Kpn2I TCCGGA 1 cut(s) 287
Ksp22I TGATCA 1 cut(s) 337
Kzo9I GATC 3 cut(s) 119, 337, 376
LmnI GCTCC 1 cut(s) 754
LpnPI CCDG 9 cut(s) 102, 301, 372, 536, 585, 660, 711, 765, 770
Lsp1109I GCAGC 2 cut(s) 332, 793
LweI GCATC 3 cut(s) 196, 540, 565
MaeII ACGT 3 cut(s) 47, 489, 914
MaeIII GTNAC 1 cut(s) 101
MalI GATC 3 cut(s) 121, 339, 378
MboI GATC 3 cut(s) 119, 337, 376
MboII GAAGA 3 cut(s) 47, 597, 713
MluCI AATT 1 cut(s) 611
MnlI CCTC 7 cut(s) 5, 70, 190, 391, 715, 796, 860
MroI TCCGGA 1 cut(s) 287
MseI TTAA 4 cut(s) 456, 537, 564, 576
MslI CAYNNNNRTG 1 cut(s) 242
MspA1I CMGCKG 1 cut(s) 784
MspI CCGG 4 cut(s) 288, 647, 698, 752
MspR9I CCNGG 1 cut(s) 648
MwoI GCNNNNNNNGC 3 cut(s) 326, 790, 867
NciI CCSGG 1 cut(s) 648
NdeI CATATG 1 cut(s) 218
NdeII GATC 3 cut(s) 119, 337, 376
NlaIII CATG 5 cut(s) 313, 338, 482, 511, 605
NlaIV GGNNCC 1 cut(s) 607
NspI RCATGY 1 cut(s) 482
PaeI GCATGC 1 cut(s) 482
PagI TCATGA 1 cut(s) 309
PceI AGGCCT 1 cut(s) 389
PfeI GAWTC 2 cut(s) 284, 836
PfoI TCCNGGA 1 cut(s) 646
PkrI GCNGC 2 cut(s) 322, 783
Psp1406I AACGTT 1 cut(s) 489
PspN4I GGNNCC 1 cut(s) 607
PspPI GGNCC 1 cut(s) 605
PvuII CAGCTG 1 cut(s) 784
RsaI GTAC 2 cut(s) 265, 333
RsaNI GTAC 2 cut(s) 264, 332
RseI CAYNNNNRTG 1 cut(s) 242
SaqAI TTAA 4 cut(s) 456, 537, 564, 576
SatI GCNGC 2 cut(s) 321, 782
Sau3AI GATC 3 cut(s) 119, 337, 376
Sau96I GGNCC 1 cut(s) 605
ScrFI CCNGG 1 cut(s) 648
SfaNI GCATC 3 cut(s) 196, 540, 565
SfcI CTRYAG 1 cut(s) 771
SinI GGWCC 1 cut(s) 605
SmiMI CAYNNNNRTG 1 cut(s) 242
SphI GCATGC 1 cut(s) 482
Sse9I AATT 1 cut(s) 611
SseBI AGGCCT 1 cut(s) 389
SsiI CCGC 3 cut(s) 153, 501, 793
SspI AATATT 1 cut(s) 730
StuI AGGCCT 1 cut(s) 389
StyD4I CCNGG 1 cut(s) 646
StyI CCWWGG 3 cut(s) 324, 390, 414
TaaI ACNGT 2 cut(s) 569, 889
TaiI ACGT 3 cut(s) 50, 492, 917
TaqI TCGA 3 cut(s) 170, 268, 876
TasI AATT 1 cut(s) 611
TfiI GAWTC 2 cut(s) 284, 836
Tru1I TTAA 4 cut(s) 456, 537, 564, 576
Tru9I TTAA 4 cut(s) 456, 537, 564, 576
TscAI CASTG 1 cut(s) 121
TseI GCWGC 2 cut(s) 320, 781
TspDTI ATGAA 5 cut(s) 123, 155, 205, 326, 496
TspRI CASTG 1 cut(s) 121
VpaK11BI GGWCC 1 cut(s) 605
XceI RCATGY 1 cut(s) 482
XcmI CCANNNNNNNNNTGG 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.