Rh5BG130200

Protein phosphatase 2C 55

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0004533
Physical Location & Seq
Forward (+)
12798088 .. 12798759
672 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG130200.1

Sequence Viewer

Length: 672 bp
ATGATCCTACTCGATCGATCAAAAGAAAGAACCATTATGAAAATGGTTTGTGGGTCATTTTATTTGTCCAAAGAACAAAGAGACCGGCCGATAGAGGGCGATGATGCTCACTTCATTTGTGCAGAAGAGAACATTATCGGTGTGGCGGATGGCGTGGGTGGTACTCGAAAGAATGGCATTGATTCCGAAATTTACGCAAGGAAGCTCATGAACAATGCGGTCACGGCTGTACATAATCTACATAGAAGATCAGGAGATAGTAATGTTGATCCGATAGAGGTCTTGAATGAACCTTATGCAAACACCAAGGATGTTGATGGGTCATCAACAGCTTGTATTGATGCGCTTAATAACAAAGATGGAAAATTGCATGCTGTGAATGTTGGGGATAGTGGTTTTATGTTGTTTAGAAACAAGAAGTGCATCGACAAGTCTGAAATACAGGTACATCGTTTTAACTGTCCTGTTCAATTGGGGAGAAAAACAAAGGATGGTCCCCAATCGGCTGTGGAGAGAAAACCAATTGAGGTTATCCCAGGAGATGTTATAGTTCTTGGGACAGATGGCCTGCTTGACAATGTGTGGCCAGAAGAAATAGAGCAGGTTTTGGAGGAGAATACCATAGAAGGTTTTGTGGAATCGGAGCAGTTGGCGTGGACTATAGCAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoIIE

Protein Analysis

223

Amino Acids

24.72

Weight (kDa)

4.93

Isoelectric Point (pI)

53.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoIIE PF07228 66 - 208 8e-07 Stage II sporulation protein E (SpoIIE)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 592
AciI CCGC 2 cut(s) 146, 218
AclWI GGATC 1 cut(s) 263
AcoI YGGCCR 2 cut(s) 86, 584
AcsI RAATTY 1 cut(s) 189
AfaI GTAC 3 cut(s) 163, 231, 447
AfiI CCNNNNNNNGG 1 cut(s) 95
AgsI TTSAA 2 cut(s) 286, 470
AjnI CCWGG 1 cut(s) 535
AluBI AGCT 2 cut(s) 205, 332
AluI AGCT 2 cut(s) 205, 332
Alw26I GTCTC 1 cut(s) 75
AlwI GGATC 1 cut(s) 263
AoxI GGCC 3 cut(s) 86, 565, 584
ApoI RAATTY 1 cut(s) 189
AspLEI GCGC 1 cut(s) 346
AspS9I GGNCC 1 cut(s) 494
AvaII GGWCC 1 cut(s) 494
BalI TGGCCA 1 cut(s) 586
BccI CCATC 5 cut(s) 143, 311, 353, 485, 557
BceAI ACGGC 1 cut(s) 240
BciT130I CCWGG 1 cut(s) 537
BcoDI GTCTC 1 cut(s) 75
BfmI CTRYAG 1 cut(s) 660
BfuAI ACCTGC 1 cut(s) 592
Bme1390I CCNGG 1 cut(s) 537
Bme18I GGWCC 1 cut(s) 494
BmgT120I GGNCC 1 cut(s) 494
BmiI GGNNCC 1 cut(s) 496
BmrFI CCNGG 1 cut(s) 537
BmsI GCATC 3 cut(s) 94, 331, 432
Bsa29I ATCGAT 1 cut(s) 16
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 2 cut(s) 306, 535
Bsc4I CCNNNNNNNGG 1 cut(s) 95
Bse118I RCCGGY 1 cut(s) 84
BseBI CCWGG 1 cut(s) 537
BseCI ATCGAT 1 cut(s) 16
BseDI CCNNGG 2 cut(s) 306, 535
BseGI GGATG 3 cut(s) 154, 316, 496
BseLI CCNNNNNNNGG 1 cut(s) 95
BseRI GAGGAG 1 cut(s) 626
BseX3I CGGCCG 1 cut(s) 86
BsgI GTGCAG 1 cut(s) 141
Bsh1285I CGRYCG 2 cut(s) 16, 89
BshFI GGCC 3 cut(s) 88, 567, 586
BshVI ATCGAT 1 cut(s) 16
BsiEI CGRYCG 2 cut(s) 16, 89
BsiSI CCGG 1 cut(s) 85
BslFI GGGAC 2 cut(s) 480, 571
BslI CCNNNNNNNGG 1 cut(s) 95
BsmAI GTCTC 1 cut(s) 75
BsmFI GGGAC 2 cut(s) 480, 571
BsnI GGCC 3 cut(s) 88, 567, 586
Bso31I GGTCTC 1 cut(s) 75
Bsp1407I TGTACA 1 cut(s) 229
Bsp143I GATC 5 cut(s) 3, 13, 17, 248, 268
BspACI CCGC 2 cut(s) 146, 218
BspANI GGCC 3 cut(s) 88, 567, 586
BspDI ATCGAT 1 cut(s) 16
BspHI TCATGA 1 cut(s) 207
BspLI GGNNCC 1 cut(s) 496
BspMI ACCTGC 1 cut(s) 592
BspPI GGATC 1 cut(s) 263
BspTNI GGTCTC 1 cut(s) 75
BsrFI RCCGGY 1 cut(s) 84
BsrGI TGTACA 1 cut(s) 229
BssAI RCCGGY 1 cut(s) 84
BssECI CCNNGG 2 cut(s) 306, 535
BssMI GATC 5 cut(s) 3, 13, 17, 248, 268
BssT1I CCWWGG 1 cut(s) 306
Bst2UI CCWGG 1 cut(s) 537
Bst4CI ACNGT 1 cut(s) 461
Bst6I CTCTTC 1 cut(s) 120
BstAUI TGTACA 1 cut(s) 229
BstC8I GCNNGC 2 cut(s) 372, 569
BstF5I GGATG 3 cut(s) 154, 316, 496
BstHHI GCGC 1 cut(s) 346
BstKTI GATC 5 cut(s) 6, 16, 20, 251, 271
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 5 cut(s) 3, 13, 17, 248, 268
BstMCI CGRYCG 2 cut(s) 16, 89
BstMWI GCNNNNNNNGC 1 cut(s) 224
BstNI CCWGG 1 cut(s) 537
BstNSI RCATGY 1 cut(s) 374
BstSCI CCNGG 1 cut(s) 535
BstSFI CTRYAG 1 cut(s) 660
BstZI CGGCCG 1 cut(s) 86
Bsu15I ATCGAT 1 cut(s) 16
BsuRI GGCC 3 cut(s) 88, 567, 586
BsuTUI ATCGAT 1 cut(s) 16
BtgZI GCGATG 1 cut(s) 114
BtsCI GGATG 3 cut(s) 154, 316, 496
BveI ACCTGC 1 cut(s) 592
Cac8I GCNNGC 2 cut(s) 372, 569
CciI TCATGA 1 cut(s) 207
CfoI GCGC 1 cut(s) 346
Cfr10I RCCGGY 1 cut(s) 84
Cfr13I GGNCC 1 cut(s) 494
ClaI ATCGAT 1 cut(s) 16
Csp6I GTAC 3 cut(s) 162, 230, 446
CviAII CATG 2 cut(s) 208, 371
CviJI RGCY 7 cut(s) 88, 205, 227, 332, 506, 567, 586
CviKI_1 RGCY 7 cut(s) 88, 205, 227, 332, 506, 567, 586
CviQI GTAC 3 cut(s) 162, 230, 446
DpnI GATC 5 cut(s) 5, 15, 19, 250, 270
DpnII GATC 5 cut(s) 3, 13, 17, 248, 268
EaeI YGGCCR 2 cut(s) 86, 584
EagI CGGCCG 1 cut(s) 86
Eam1104I CTCTTC 1 cut(s) 120
EarI CTCTTC 1 cut(s) 120
EciI GGCGGA 1 cut(s) 161
EclXI CGGCCG 1 cut(s) 86
Eco130I CCWWGG 1 cut(s) 306
Eco31I GGTCTC 1 cut(s) 75
Eco47I GGWCC 1 cut(s) 494
Eco52I CGGCCG 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 535
EcoT14I CCWWGG 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 2 cut(s) 211, 374
FaqI GGGAC 2 cut(s) 480, 571
FatI CATG 2 cut(s) 207, 370
FokI GGATG 3 cut(s) 161, 323, 503
GlaI GCGC 1 cut(s) 345
HaeIII GGCC 3 cut(s) 88, 567, 586
HapII CCGG 1 cut(s) 85
HhaI GCGC 1 cut(s) 346
Hin1II CATG 2 cut(s) 211, 374
Hin6I GCGC 1 cut(s) 344
HinP1I GCGC 1 cut(s) 344
HinfI GANTC 2 cut(s) 182, 638
HpaII CCGG 1 cut(s) 85
Hpy166II GTNNAC 1 cut(s) 657
Hpy188I TCNGA 4 cut(s) 187, 273, 436, 643
Hpy188III TCNNGA 3 cut(s) 208, 252, 283
Hpy8I GTNNAC 1 cut(s) 657
HpyAV CCTTC 1 cut(s) 620
HpyCH4III ACNGT 1 cut(s) 461
HpyCH4V TGCA 4 cut(s) 122, 299, 370, 423
HpyF10VI GCNNNNNNNGC 1 cut(s) 224
Hsp92II CATG 2 cut(s) 211, 374
HspAI GCGC 1 cut(s) 344
Kzo9I GATC 5 cut(s) 3, 13, 17, 248, 268
LmnI GCTCC 1 cut(s) 643
LpnPI CCDG 9 cut(s) 98, 237, 428, 477, 522, 549, 581, 587, 600
LweI GCATC 3 cut(s) 94, 331, 432
MaeIII GTNAC 1 cut(s) 220
MalI GATC 5 cut(s) 5, 15, 19, 250, 270
MboI GATC 5 cut(s) 3, 13, 17, 248, 268
MboII GAAGA 3 cut(s) 137, 258, 602
MfeI CAATTG 2 cut(s) 470, 522
MlsI TGGCCA 1 cut(s) 586
MluCI AATT 4 cut(s) 189, 365, 470, 522
MluNI TGGCCA 1 cut(s) 586
MnlI CCTC 4 cut(s) 88, 271, 520, 604
Mox20I TGGCCA 1 cut(s) 586
MscI TGGCCA 1 cut(s) 586
MseI TTAA 2 cut(s) 348, 456
Msp20I TGGCCA 1 cut(s) 586
MspI CCGG 1 cut(s) 85
MspR9I CCNGG 1 cut(s) 537
MunI CAATTG 2 cut(s) 470, 522
MvaI CCWGG 1 cut(s) 537
MwoI GCNNNNNNNGC 1 cut(s) 224
NdeII GATC 5 cut(s) 3, 13, 17, 248, 268
NlaIII CATG 2 cut(s) 211, 374
NlaIV GGNNCC 1 cut(s) 496
NmuCI GTSAC 1 cut(s) 220
NspI RCATGY 1 cut(s) 374
PaeI GCATGC 1 cut(s) 374
PagI TCATGA 1 cut(s) 207
PfeI GAWTC 2 cut(s) 182, 638
Ple19I CGATCG 1 cut(s) 16
Psp6I CCWGG 1 cut(s) 535
PspGI CCWGG 1 cut(s) 535
PspN4I GGNNCC 1 cut(s) 496
PspPI GGNCC 1 cut(s) 494
PvuI CGATCG 1 cut(s) 16
RsaI GTAC 3 cut(s) 163, 231, 447
RsaNI GTAC 3 cut(s) 162, 230, 446
SaqAI TTAA 2 cut(s) 348, 456
Sau3AI GATC 5 cut(s) 3, 13, 17, 248, 268
Sau96I GGNCC 1 cut(s) 494
ScrFI CCNGG 1 cut(s) 537
SetI ASST 8 cut(s) 207, 282, 295, 334, 447, 531, 606, 631
SfaNI GCATC 3 cut(s) 94, 331, 432
SfcI CTRYAG 1 cut(s) 660
SinI GGWCC 1 cut(s) 494
SphI GCATGC 1 cut(s) 374
Sse9I AATT 4 cut(s) 189, 365, 470, 522
SsiI CCGC 2 cut(s) 146, 218
StyD4I CCNGG 1 cut(s) 535
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 1 cut(s) 461
TaqI TCGA 4 cut(s) 12, 16, 166, 426
TasI AATT 4 cut(s) 189, 365, 470, 522
TatI WGTACW 1 cut(s) 229
TfiI GAWTC 2 cut(s) 182, 638
Tru1I TTAA 2 cut(s) 348, 456
Tru9I TTAA 2 cut(s) 348, 456
TseFI GTSAC 1 cut(s) 220
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 4 cut(s) 53, 103, 224, 303
VpaK11BI GGWCC 1 cut(s) 494
XapI RAATTY 1 cut(s) 189
XceI RCATGY 1 cut(s) 374
XcmI CCANNNNNNNNNTGG 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.