Rh5DG134400

Protein phosphatase 2C 55

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004533
Physical Location & Seq
Reverse (-)
13335150 .. 13336515
1366 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG134400.1

Sequence Viewer

Length: 891 bp
ATGAATTGGCTCTCGAAGATACCTAGGGTTATGAATTGGCTCTCGAAGATACCTAGGGTTGCCGAAATCATTTGGGTAAACCTAGAGAGCGACCCAACTCCATTAAAAGAAGGAACCACACAGAAGATGGTCTGTGGGTCTTTTTATTTGCCCAAAGAAAGTGAAAATCGATCGCTACTAGGCGAAGACGCTCACTTCATTTGTGCAAAAGAGAACACCATTGGTGTTGCGGATGGTGTTGGCGGGTGGGCTAAGTATGGCGTAGATGCTGGAGAGTATGCAAGGAAGCTCATGGACAACTCAGTCATGGCTGTACATAACCAACATAGAAAATCTGGAGATGTTGATGTCGATCCGATACAAATCTTGCATGAAGCCTACGGAAACACCAAGAATATTGCTGGGACATCAACAGCTTGCATCGTCACGCTTAGTAACGAAGGTAGAACCTTGCATGCTGTGAATGTTGGGGATAGTGGTTTCATGCTGTTTAGAGACAAGAAGTGTGTGTACATTTCGCCAATTCGACAGAGTTACTTTAACTGTCCTCTTCAATTGGGGACAGGATCAGGATATACTCCCCAGTCGGCTACTGAGATTAAAGTTAGGGTACTTCGGCCGGGAGATGTCATAGTTCTTGGGACAGATGGGTTACTGGACAATGTGTCGCCGGGAGAAATAGAGAAAGTTCTGGAGGAGAATACCATAGAAGGTTTTGTGGAACCGGAGATGTTGGCATGGATCATAGCAGAGAGGCTGGCTTTACGCAACTCCGAGGATGCAGGAAGGTTGACTCCTTTTTCAATTGCAGCTCATAAGGCCGGAGTCGAGCACATTGGAGGTAAGAGGGATGATATCACGGTCATTGTGGGTCAAATACTTGCAGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoIIE

Protein Analysis

296

Amino Acids

32.32

Weight (kDa)

5.56

Isoelectric Point (pI)

35.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoIIE PF07228 110 - 246 1.3e-06 Stage II sporulation protein E (SpoIIE)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 302
AciI CCGC 2 cut(s) 230, 243
AclWI GGATC 3 cut(s) 347, 574, 749
AcoI YGGCCR 1 cut(s) 617
AfaI GTAC 3 cut(s) 315, 512, 612
AgsI TTSAA 2 cut(s) 554, 804
AhdI GACNNNNNGTC 1 cut(s) 664
AluBI AGCT 3 cut(s) 289, 416, 812
AluI AGCT 3 cut(s) 289, 416, 812
Alw21I GWGCWC 1 cut(s) 834
Alw26I GTCTC 1 cut(s) 489
AlwI GGATC 3 cut(s) 347, 574, 749
AoxI GGCC 2 cut(s) 617, 819
ApeKI GCWGC 2 cut(s) 809, 884
AspA2I CCTAGG 2 cut(s) 23, 53
AsuC2I CCSGG 2 cut(s) 621, 672
AvrII CCTAGG 2 cut(s) 23, 53
BarI GAAGNNNNNNTAC 2 cut(s) 494, 526
BbsI GAAGAC 1 cut(s) 192
Bbv12I GWGCWC 1 cut(s) 834
BbvI GCAGC 1 cut(s) 821
BccI CCATC 3 cut(s) 121, 227, 641
BcnI CCSGG 2 cut(s) 621, 672
BcoDI GTCTC 1 cut(s) 489
BfaI CTAG 4 cut(s) 24, 54, 83, 179
BisI GCNGC 2 cut(s) 810, 885
BlnI CCTAGG 2 cut(s) 23, 53
BlsI GCNGC 2 cut(s) 811, 886
Bme1390I CCNGG 2 cut(s) 621, 672
BmeRI GACNNNNNGTC 1 cut(s) 664
BmiI GGNNCC 2 cut(s) 115, 723
BmrFI CCNGG 2 cut(s) 621, 672
BmrI ACTGGG 1 cut(s) 577
BmsI GCATC 3 cut(s) 256, 429, 769
BmuI ACTGGG 1 cut(s) 577
BpiI GAAGAC 1 cut(s) 192
BpmI CTGGAG 3 cut(s) 291, 357, 713
BpuMI CCSGG 2 cut(s) 621, 672
Bsa29I ATCGAT 1 cut(s) 169
BsaBI GATNNNNATC 2 cut(s) 351, 362
BsaJI CCNNGG 3 cut(s) 23, 53, 774
BsaWI WCCGGW 1 cut(s) 724
Bse1I ACTGG 2 cut(s) 583, 660
Bse8I GATNNNNATC 2 cut(s) 351, 362
BseCI ATCGAT 1 cut(s) 169
BseDI CCNNGG 3 cut(s) 23, 53, 774
BseGI GGATG 3 cut(s) 238, 784, 856
BseJI GATNNNNATC 2 cut(s) 351, 362
BseMII CTCAG 2 cut(s) 315, 585
BseNI ACTGG 2 cut(s) 583, 660
BseRI GAGGAG 1 cut(s) 710
BseX3I CGGCCG 1 cut(s) 617
BseXI GCAGC 1 cut(s) 821
BseYI CCCAGC 1 cut(s) 401
Bsh1285I CGRYCG 2 cut(s) 173, 620
BshFI GGCC 2 cut(s) 619, 821
BshVI ATCGAT 1 cut(s) 169
BsiEI CGRYCG 2 cut(s) 173, 620
BsiHKAI GWGCWC 1 cut(s) 834
BsiSI CCGG 4 cut(s) 620, 671, 725, 822
BslFI GGGAC 3 cut(s) 418, 574, 655
BsmAI GTCTC 1 cut(s) 489
BsmFI GGGAC 3 cut(s) 418, 574, 655
BsnI GGCC 2 cut(s) 619, 821
Bsp1286I GDGCHC 1 cut(s) 834
Bsp1407I TGTACA 2 cut(s) 313, 510
Bsp143I GATC 4 cut(s) 170, 352, 566, 741
BspACI CCGC 2 cut(s) 230, 243
BspANI GGCC 2 cut(s) 619, 821
BspCNI CTCAG 2 cut(s) 314, 586
BspDI ATCGAT 1 cut(s) 169
BspLI GGNNCC 2 cut(s) 115, 723
BspPI GGATC 3 cut(s) 347, 574, 749
BsrGI TGTACA 2 cut(s) 313, 510
BsrI ACTGG 2 cut(s) 583, 660
BssECI CCNNGG 3 cut(s) 23, 53, 774
BssMI GATC 4 cut(s) 170, 352, 566, 741
BssT1I CCWWGG 2 cut(s) 23, 53
Bst4CI ACNGT 2 cut(s) 545, 862
Bst6I CTCTTC 1 cut(s) 555
BstAUI TGTACA 2 cut(s) 313, 510
BstC8I GCNNGC 3 cut(s) 418, 456, 759
BstDEI CTNAG 4 cut(s) 252, 301, 431, 594
BstF5I GGATG 3 cut(s) 238, 784, 856
BstKTI GATC 4 cut(s) 173, 355, 569, 744
BstMAI GTCTC 1 cut(s) 489
BstMBI GATC 4 cut(s) 170, 352, 566, 741
BstMCI CGRYCG 2 cut(s) 173, 620
BstMWI GCNNNNNNNGC 1 cut(s) 818
BstNSI RCATGY 1 cut(s) 458
BstSCI CCNGG 2 cut(s) 619, 670
BstV1I GCAGC 1 cut(s) 821
BstV2I GAAGAC 1 cut(s) 192
BstZI CGGCCG 1 cut(s) 617
Bsu15I ATCGAT 1 cut(s) 169
BsuRI GGCC 2 cut(s) 619, 821
BsuTUI ATCGAT 1 cut(s) 169
BtsCI GGATG 3 cut(s) 238, 784, 856
Cac8I GCNNGC 3 cut(s) 418, 456, 759
ClaI ATCGAT 1 cut(s) 169
CseI GACGC 1 cut(s) 197
Csp6I GTAC 3 cut(s) 314, 511, 611
CviAII CATG 6 cut(s) 292, 307, 371, 455, 484, 738
CviQI GTAC 3 cut(s) 314, 511, 611
DdeI CTNAG 4 cut(s) 252, 301, 431, 594
DpnI GATC 4 cut(s) 172, 354, 568, 743
DpnII GATC 4 cut(s) 170, 352, 566, 741
DrdI GACNNNNNNGTC 1 cut(s) 302
DriI GACNNNNNGTC 1 cut(s) 664
DseDI GACNNNNNNGTC 1 cut(s) 302
EaeI YGGCCR 1 cut(s) 617
EagI CGGCCG 1 cut(s) 617
Eam1104I CTCTTC 1 cut(s) 555
Eam1105I GACNNNNNGTC 1 cut(s) 664
EarI CTCTTC 1 cut(s) 555
EclXI CGGCCG 1 cut(s) 617
Eco130I CCWWGG 2 cut(s) 23, 53
Eco32I GATATC 1 cut(s) 856
Eco52I CGGCCG 1 cut(s) 617
EcoRV GATATC 1 cut(s) 856
EcoT14I CCWWGG 2 cut(s) 23, 53
ErhI CCWWGG 2 cut(s) 23, 53
FaeI CATG 6 cut(s) 295, 310, 374, 458, 487, 741
FaqI GGGAC 3 cut(s) 418, 574, 655
FatI CATG 6 cut(s) 291, 306, 370, 454, 483, 737
FauI CCCGC 1 cut(s) 236
Fnu4HI GCNGC 2 cut(s) 810, 885
FokI GGATG 3 cut(s) 245, 791, 863
Fsp4HI GCNGC 2 cut(s) 810, 885
FspBI CTAG 4 cut(s) 24, 54, 83, 179
GluI GCNGC 2 cut(s) 810, 885
GsaI CCCAGC 1 cut(s) 405
GsuI CTGGAG 3 cut(s) 291, 357, 713
HaeIII GGCC 2 cut(s) 619, 821
HapII CCGG 4 cut(s) 620, 671, 725, 822
HgaI GACGC 1 cut(s) 197
Hin1II CATG 6 cut(s) 295, 310, 374, 458, 487, 741
HincII GTYRAC 1 cut(s) 792
HindII GTYRAC 1 cut(s) 792
HinfI GANTC 2 cut(s) 793, 825
HpaII CCGG 4 cut(s) 620, 671, 725, 822
Hpy166II GTNNAC 3 cut(s) 79, 511, 792
Hpy188I TCNGA 2 cut(s) 357, 775
Hpy188III TCNNGA 5 cut(s) 13, 43, 336, 570, 692
Hpy8I GTNNAC 3 cut(s) 79, 511, 792
HpyAV CCTTC 4 cut(s) 104, 434, 704, 780
HpyCH4III ACNGT 2 cut(s) 545, 862
HpyCH4V TGCA 8 cut(s) 206, 281, 370, 420, 454, 782, 809, 884
HpyF10VI GCNNNNNNNGC 1 cut(s) 818
HpyF3I CTNAG 4 cut(s) 252, 301, 431, 594
Hsp92II CATG 6 cut(s) 295, 310, 374, 458, 487, 741
Kzo9I GATC 4 cut(s) 170, 352, 566, 741
Lsp1109I GCAGC 1 cut(s) 821
LweI GCATC 3 cut(s) 256, 429, 769
MaeI CTAG 4 cut(s) 24, 54, 83, 179
MaeIII GTNAC 4 cut(s) 424, 434, 533, 651
MalI GATC 4 cut(s) 172, 354, 568, 743
MboI GATC 4 cut(s) 170, 352, 566, 741
MboII GAAGA 5 cut(s) 28, 58, 136, 197, 542
MfeI CAATTG 2 cut(s) 554, 804
MhlI GDGCHC 1 cut(s) 834
MluCI AATT 5 cut(s) 4, 34, 522, 554, 804
MlyI GAGTC 2 cut(s) 787, 834
MnlI CCTC 6 cut(s) 558, 688, 747, 769, 833, 840
MseI TTAA 3 cut(s) 104, 540, 600
MspI CCGG 4 cut(s) 620, 671, 725, 822
MspR9I CCNGG 2 cut(s) 621, 672
MunI CAATTG 2 cut(s) 554, 804
MwoI GCNNNNNNNGC 1 cut(s) 818
NciI CCSGG 2 cut(s) 621, 672
NdeII GATC 4 cut(s) 170, 352, 566, 741
NlaIII CATG 6 cut(s) 295, 310, 374, 458, 487, 741
NlaIV GGNNCC 2 cut(s) 115, 723
NmuCI GTSAC 1 cut(s) 424
NspI RCATGY 1 cut(s) 458
PaeI GCATGC 1 cut(s) 458
PkrI GCNGC 2 cut(s) 811, 886
Ple19I CGATCG 1 cut(s) 173
PleI GAGTC 2 cut(s) 787, 833
PpsI GAGTC 2 cut(s) 787, 833
PspFI CCCAGC 1 cut(s) 401
PspN4I GGNNCC 2 cut(s) 115, 723
PvuI CGATCG 1 cut(s) 173
RsaI GTAC 3 cut(s) 315, 512, 612
RsaNI GTAC 3 cut(s) 314, 511, 611
SaqAI TTAA 3 cut(s) 104, 540, 600
SatI GCNGC 2 cut(s) 810, 885
Sau3AI GATC 4 cut(s) 170, 352, 566, 741
SchI GAGTC 2 cut(s) 787, 834
ScrFI CCNGG 2 cut(s) 621, 672
SduI GDGCHC 1 cut(s) 834
SfaNI GCATC 3 cut(s) 256, 429, 769
SphI GCATGC 1 cut(s) 458
Sse9I AATT 5 cut(s) 4, 34, 522, 554, 804
SsiI CCGC 2 cut(s) 230, 243
SspI AATATT 1 cut(s) 397
SspMI CTAG 4 cut(s) 24, 54, 83, 179
StyD4I CCNGG 2 cut(s) 619, 670
StyI CCWWGG 2 cut(s) 23, 53
TaaI ACNGT 2 cut(s) 545, 862
TaqI TCGA 6 cut(s) 14, 44, 169, 351, 526, 828
TasI AATT 5 cut(s) 4, 34, 522, 554, 804
TatI WGTACW 2 cut(s) 313, 510
Tru1I TTAA 3 cut(s) 104, 540, 600
Tru9I TTAA 3 cut(s) 104, 540, 600
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 2 cut(s) 809, 884
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 5 cut(s) 17, 47, 187, 387, 472
TspGWI ACGGA 1 cut(s) 396
XceI RCATGY 1 cut(s) 458
XcmI CCANNNNNNNNNTGG 1 cut(s) 124
XmaJI CCTAGG 2 cut(s) 23, 53
XspI CTAG 4 cut(s) 24, 54, 83, 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.