Rh5CG144700

Protein phosphatase 2C 55

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0004533
Physical Location & Seq
Reverse (-)
13029783 .. 13031112
1330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG144700.1

Sequence Viewer

Length: 861 bp
ATGAATTGGCTCTCGAAGATACCTAGGGTTGCCGAAATCATTTGGGTACACCTAGAGAGCGACCCAACTCCATTAAAAGAAGGAACCACACTGAAGATGGTCTGTGGGTCTTTTTATTTGCCCAAAGAAAGTGAAAATCGATCGCTACTAGGCGAAGACGCTCAATTCATTTGTGCAAAAGAGAACACCATTGGTGTTGCGGATGGTGTTGGCGGGTGGGCTAAGTATGGCGTAGATGCTGGAGAGTATGCAAGGAAGCTCATGGACAACTCAGTCATGGCTGTACATAACCAACATAGAAAATCTGGAGATGTTGATGTCGATCCGATACAAATCTTGCATGAAGCCTACGGAAACACCAAGAATATTGCTGGGACATCAACAGCTTGCATCGTCACGCTTAGTAACGAAGGTAGAGCCTTGCATGCTGTGAATGTTGGGGATAGTGGTTTCATGCTGTTTAGAGACAAGAAGTGTGTGTACATTTCGCCAATTCGACAGAGTTACTTTAACTGTCCTCTTCAATTGGGGACAGGATCAGGATATACTCCCCAGTCGGCTACTGAGATTAAAGTTAGGGTACTTCGGCCGGGAGATGTCATAGTTCTTGGGACAGATGGGTTACTGGACAATGTGTCGCCGGGAGAAATAGAGAAAGTTCTGGAGGAGAATACCATAGAAGGTTTTGTGGAACCGGAGATGTTGGCATGGATCATAGCAGAGAGGCTGGCTTTACGCAACTCCGAGGATGCAGGAAGGTTGACTCCTTTTTCAATTGCAGCTCATAAGGCCGGAGTCGAGCACATTGGAGGTAAGAGGGATGATATCACGGTCATTGTAGGTCAAATACTTGCAGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoIIE

Protein Analysis

286

Amino Acids

31.06

Weight (kDa)

5.33

Isoelectric Point (pI)

36.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoIIE PF07228 100 - 236 4e-06 Stage II sporulation protein E (SpoIIE)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 272
AciI CCGC 2 cut(s) 200, 213
AclWI GGATC 3 cut(s) 317, 544, 719
AcoI YGGCCR 1 cut(s) 587
AcuI CTGAAG 1 cut(s) 113
AfaI GTAC 4 cut(s) 48, 285, 482, 582
AgsI TTSAA 2 cut(s) 524, 774
AhdI GACNNNNNGTC 1 cut(s) 634
AluBI AGCT 3 cut(s) 259, 386, 782
AluI AGCT 3 cut(s) 259, 386, 782
Alw21I GWGCWC 1 cut(s) 804
Alw26I GTCTC 1 cut(s) 459
AlwI GGATC 3 cut(s) 317, 544, 719
AoxI GGCC 2 cut(s) 587, 789
ApeKI GCWGC 2 cut(s) 779, 854
AspA2I CCTAGG 1 cut(s) 23
AsuC2I CCSGG 2 cut(s) 591, 642
AvrII CCTAGG 1 cut(s) 23
BarI GAAGNNNNNNTAC 2 cut(s) 464, 496
BbsI GAAGAC 1 cut(s) 162
Bbv12I GWGCWC 1 cut(s) 804
BbvI GCAGC 1 cut(s) 791
BccI CCATC 3 cut(s) 91, 197, 611
BcnI CCSGG 2 cut(s) 591, 642
BcoDI GTCTC 1 cut(s) 459
BfaI CTAG 3 cut(s) 24, 53, 149
BisI GCNGC 2 cut(s) 780, 855
BlnI CCTAGG 1 cut(s) 23
BlsI GCNGC 2 cut(s) 781, 856
Bme1390I CCNGG 2 cut(s) 591, 642
BmeRI GACNNNNNGTC 1 cut(s) 634
BmiI GGNNCC 2 cut(s) 85, 693
BmrFI CCNGG 2 cut(s) 591, 642
BmrI ACTGGG 1 cut(s) 547
BmsI GCATC 3 cut(s) 226, 399, 739
BmuI ACTGGG 1 cut(s) 547
BpiI GAAGAC 1 cut(s) 162
BpmI CTGGAG 3 cut(s) 261, 327, 683
BpuMI CCSGG 2 cut(s) 591, 642
Bsa29I ATCGAT 1 cut(s) 139
BsaBI GATNNNNATC 2 cut(s) 321, 332
BsaJI CCNNGG 2 cut(s) 23, 744
BsaWI WCCGGW 1 cut(s) 694
Bse1I ACTGG 2 cut(s) 553, 630
Bse8I GATNNNNATC 2 cut(s) 321, 332
BseCI ATCGAT 1 cut(s) 139
BseDI CCNNGG 2 cut(s) 23, 744
BseGI GGATG 3 cut(s) 208, 754, 826
BseJI GATNNNNATC 2 cut(s) 321, 332
BseMII CTCAG 2 cut(s) 285, 555
BseNI ACTGG 2 cut(s) 553, 630
BseRI GAGGAG 1 cut(s) 680
BseX3I CGGCCG 1 cut(s) 587
BseXI GCAGC 1 cut(s) 791
BseYI CCCAGC 1 cut(s) 371
Bsh1285I CGRYCG 2 cut(s) 143, 590
BshFI GGCC 2 cut(s) 589, 791
BshVI ATCGAT 1 cut(s) 139
BsiEI CGRYCG 2 cut(s) 143, 590
BsiHKAI GWGCWC 1 cut(s) 804
BsiSI CCGG 4 cut(s) 590, 641, 695, 792
BslFI GGGAC 3 cut(s) 388, 544, 625
BsmAI GTCTC 1 cut(s) 459
BsmFI GGGAC 3 cut(s) 388, 544, 625
BsnI GGCC 2 cut(s) 589, 791
Bsp1286I GDGCHC 1 cut(s) 804
Bsp1407I TGTACA 2 cut(s) 283, 480
Bsp143I GATC 4 cut(s) 140, 322, 536, 711
BspACI CCGC 2 cut(s) 200, 213
BspANI GGCC 2 cut(s) 589, 791
BspCNI CTCAG 2 cut(s) 284, 556
BspDI ATCGAT 1 cut(s) 139
BspLI GGNNCC 2 cut(s) 85, 693
BspPI GGATC 3 cut(s) 317, 544, 719
BsrGI TGTACA 2 cut(s) 283, 480
BsrI ACTGG 2 cut(s) 553, 630
BssECI CCNNGG 2 cut(s) 23, 744
BssMI GATC 4 cut(s) 140, 322, 536, 711
BssT1I CCWWGG 1 cut(s) 23
Bst4CI ACNGT 2 cut(s) 515, 832
Bst6I CTCTTC 1 cut(s) 525
BstAUI TGTACA 2 cut(s) 283, 480
BstC8I GCNNGC 3 cut(s) 388, 426, 729
BstDEI CTNAG 4 cut(s) 222, 271, 401, 564
BstF5I GGATG 3 cut(s) 208, 754, 826
BstKTI GATC 4 cut(s) 143, 325, 539, 714
BstMAI GTCTC 1 cut(s) 459
BstMBI GATC 4 cut(s) 140, 322, 536, 711
BstMCI CGRYCG 2 cut(s) 143, 590
BstMWI GCNNNNNNNGC 2 cut(s) 425, 788
BstNSI RCATGY 1 cut(s) 428
BstSCI CCNGG 2 cut(s) 589, 640
BstV1I GCAGC 1 cut(s) 791
BstV2I GAAGAC 1 cut(s) 162
BstZI CGGCCG 1 cut(s) 587
Bsu15I ATCGAT 1 cut(s) 139
BsuRI GGCC 2 cut(s) 589, 791
BsuTUI ATCGAT 1 cut(s) 139
BtsCI GGATG 3 cut(s) 208, 754, 826
BtsIMutI CAGTG 1 cut(s) 89
Cac8I GCNNGC 3 cut(s) 388, 426, 729
ClaI ATCGAT 1 cut(s) 139
CseI GACGC 1 cut(s) 167
Csp6I GTAC 4 cut(s) 47, 284, 481, 581
CviAII CATG 6 cut(s) 262, 277, 341, 425, 454, 708
CviQI GTAC 4 cut(s) 47, 284, 481, 581
DdeI CTNAG 4 cut(s) 222, 271, 401, 564
DpnI GATC 4 cut(s) 142, 324, 538, 713
DpnII GATC 4 cut(s) 140, 322, 536, 711
DrdI GACNNNNNNGTC 1 cut(s) 272
DriI GACNNNNNGTC 1 cut(s) 634
DseDI GACNNNNNNGTC 1 cut(s) 272
EaeI YGGCCR 1 cut(s) 587
EagI CGGCCG 1 cut(s) 587
Eam1104I CTCTTC 1 cut(s) 525
Eam1105I GACNNNNNGTC 1 cut(s) 634
EarI CTCTTC 1 cut(s) 525
EclXI CGGCCG 1 cut(s) 587
Eco130I CCWWGG 1 cut(s) 23
Eco32I GATATC 1 cut(s) 826
Eco52I CGGCCG 1 cut(s) 587
Eco57I CTGAAG 1 cut(s) 113
EcoRV GATATC 1 cut(s) 826
EcoT14I CCWWGG 1 cut(s) 23
ErhI CCWWGG 1 cut(s) 23
FaeI CATG 6 cut(s) 265, 280, 344, 428, 457, 711
FaqI GGGAC 3 cut(s) 388, 544, 625
FatI CATG 6 cut(s) 261, 276, 340, 424, 453, 707
FauI CCCGC 1 cut(s) 206
Fnu4HI GCNGC 2 cut(s) 780, 855
FokI GGATG 3 cut(s) 215, 761, 833
Fsp4HI GCNGC 2 cut(s) 780, 855
FspBI CTAG 3 cut(s) 24, 53, 149
GluI GCNGC 2 cut(s) 780, 855
GsaI CCCAGC 1 cut(s) 375
GsuI CTGGAG 3 cut(s) 261, 327, 683
HaeIII GGCC 2 cut(s) 589, 791
HapII CCGG 4 cut(s) 590, 641, 695, 792
HgaI GACGC 1 cut(s) 167
Hin1II CATG 6 cut(s) 265, 280, 344, 428, 457, 711
HincII GTYRAC 1 cut(s) 762
HindII GTYRAC 1 cut(s) 762
HinfI GANTC 2 cut(s) 763, 795
HpaII CCGG 4 cut(s) 590, 641, 695, 792
Hpy166II GTNNAC 3 cut(s) 49, 481, 762
Hpy188I TCNGA 2 cut(s) 327, 745
Hpy188III TCNNGA 4 cut(s) 13, 306, 540, 662
Hpy8I GTNNAC 3 cut(s) 49, 481, 762
HpyAV CCTTC 4 cut(s) 74, 404, 674, 750
HpyCH4III ACNGT 2 cut(s) 515, 832
HpyCH4V TGCA 8 cut(s) 176, 251, 340, 390, 424, 752, 779, 854
HpyF10VI GCNNNNNNNGC 2 cut(s) 425, 788
HpyF3I CTNAG 4 cut(s) 222, 271, 401, 564
Hsp92II CATG 6 cut(s) 265, 280, 344, 428, 457, 711
Kzo9I GATC 4 cut(s) 140, 322, 536, 711
Lsp1109I GCAGC 1 cut(s) 791
LweI GCATC 3 cut(s) 226, 399, 739
MaeI CTAG 3 cut(s) 24, 53, 149
MaeIII GTNAC 4 cut(s) 394, 404, 503, 621
MalI GATC 4 cut(s) 142, 324, 538, 713
MboI GATC 4 cut(s) 140, 322, 536, 711
MboII GAAGA 4 cut(s) 28, 106, 167, 512
MfeI CAATTG 2 cut(s) 524, 774
MhlI GDGCHC 1 cut(s) 804
MluCI AATT 5 cut(s) 4, 164, 492, 524, 774
MlyI GAGTC 2 cut(s) 757, 804
MnlI CCTC 6 cut(s) 528, 658, 717, 739, 803, 810
MseI TTAA 3 cut(s) 74, 510, 570
MspI CCGG 4 cut(s) 590, 641, 695, 792
MspR9I CCNGG 2 cut(s) 591, 642
MunI CAATTG 2 cut(s) 524, 774
MwoI GCNNNNNNNGC 2 cut(s) 425, 788
NciI CCSGG 2 cut(s) 591, 642
NdeII GATC 4 cut(s) 140, 322, 536, 711
NlaIII CATG 6 cut(s) 265, 280, 344, 428, 457, 711
NlaIV GGNNCC 2 cut(s) 85, 693
NmuCI GTSAC 1 cut(s) 394
NspI RCATGY 1 cut(s) 428
PaeI GCATGC 1 cut(s) 428
PkrI GCNGC 2 cut(s) 781, 856
Ple19I CGATCG 1 cut(s) 143
PleI GAGTC 2 cut(s) 757, 803
PpsI GAGTC 2 cut(s) 757, 803
PspFI CCCAGC 1 cut(s) 371
PspN4I GGNNCC 2 cut(s) 85, 693
PvuI CGATCG 1 cut(s) 143
RsaI GTAC 4 cut(s) 48, 285, 482, 582
RsaNI GTAC 4 cut(s) 47, 284, 481, 581
SaqAI TTAA 3 cut(s) 74, 510, 570
SatI GCNGC 2 cut(s) 780, 855
Sau3AI GATC 4 cut(s) 140, 322, 536, 711
SchI GAGTC 2 cut(s) 757, 804
ScrFI CCNGG 2 cut(s) 591, 642
SduI GDGCHC 1 cut(s) 804
SfaNI GCATC 3 cut(s) 226, 399, 739
SphI GCATGC 1 cut(s) 428
Sse9I AATT 5 cut(s) 4, 164, 492, 524, 774
SsiI CCGC 2 cut(s) 200, 213
SspI AATATT 1 cut(s) 367
SspMI CTAG 3 cut(s) 24, 53, 149
StyD4I CCNGG 2 cut(s) 589, 640
StyI CCWWGG 1 cut(s) 23
TaaI ACNGT 2 cut(s) 515, 832
TaqI TCGA 5 cut(s) 14, 139, 321, 496, 798
TasI AATT 5 cut(s) 4, 164, 492, 524, 774
TatI WGTACW 2 cut(s) 283, 480
Tru1I TTAA 3 cut(s) 74, 510, 570
Tru9I TTAA 3 cut(s) 74, 510, 570
TscAI CASTG 1 cut(s) 96
TseFI GTSAC 1 cut(s) 394
TseI GCWGC 2 cut(s) 779, 854
Tsp45I GTSAC 1 cut(s) 394
TspDTI ATGAA 4 cut(s) 17, 157, 357, 442
TspGWI ACGGA 1 cut(s) 366
TspRI CASTG 1 cut(s) 96
XceI RCATGY 1 cut(s) 428
XcmI CCANNNNNNNNNTGG 1 cut(s) 94
XmaJI CCTAGG 1 cut(s) 23
XspI CTAG 3 cut(s) 24, 53, 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.