Rorug05G0029500

protein phosphatase 2C 55

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004533
Physical Location & Seq
Forward (+)
1883031 .. 1884860
1830 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0029500.1

Sequence Viewer

Length: 1194 bp
ATGCGCTGCTTCTCTTGTTGCAAGCCGGTGGAGGATGATCCTCCGAAATTTATAGTCAGAAAGACAGGCAGAAGGCCATATTCTAATACTGCCACGACATTGGACTCATTTTTCGCAACGCTCTCCCTTAAGTCAGGTAGCAGCAGATGCAGGAATTTGCGCAATCAGATACTCAAGGTGGGAAGTGCAAAGCAATCTGCTCGGCTTTTTACGTTCAATGAGCTTCATGCTGCAACTGAGGGCTTCAAGGCTGCTTGTTTGCTTGGAGAAGGAGGATTTGGGAAGGTGTACAAAGGGTACATAGATAGCATCAATTCTAAACAGGCTGTGGCGGTTAAGAAACTTAACAGGAAAGGAATGCAAGGAAGTAGAGAGTTTTGTGCTGAAGTTATCATGTTGAGTCTGTTTCAACACCCAAACCTTGTCAGTCTAGTAGGGTACTGTGCGGAAGGAGAGCAGAGAGTTTTAGTGTATGAATATATGGCCAGTGGATCTCTGGAGGATCACCTTCTAGGTCCGAATCATGAGCAGCCTCTTGATTGGTACACTAGGATGAGAATAGCTGAAGGAGCAGCTAAAGGACTTGAGTACTTGCATGATGTTGCCAAACCAGCAGTGATTTATCGCGATTTCAAAGCATCAAACATATTGTTGGATTCCCAATTCACTCCGAAGCTGTCTGATTTTGGACTTGCTAAGTTAGGTCCAACAGGAGACAAGTCCCATGTGTCTACAAGGGTGATGGGAACTTATGGCTATTGTGCCCCAGAGTATGCTTGCACAGGAAAGCTCACAAAAATGTCTGATGTCTACAGTTTCGGGGTTGTCTTTCTTGAGTTAATCACCGGAAGAAGAGCTGTCGATGAGACAAAACCAGAGGAAGAGCAGATCCTAGTCAGTTGGGCACAACCACTGTTGAAGGATAGAAGTAAATTTTCATTAATTGCTGATCCGTTGCTTAGGGGAAACTTTCCGGTGAAGGGTCTGCATCAAGCTCTTGCAATTGCAGCATTGTGTCTCAATGAGGAAGCTGATTGCCGACCTGTAATGTCTGATGTTGTACTTGCTCTTGAGCATTTATCCAAGCCAAGAAATGAAGTTGTCATTGATGATGAATCAAAATACAGCCCTGTGCAGCTGACCAATGTCCAATCTCTAAAACGAAGGAGTTCCGAAGCAGAGCTAGATCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoIIE

Protein Analysis

397

Amino Acids

44.05

Weight (kDa)

8.52

Isoelectric Point (pI)

39.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 86 - 294 2.6e-45 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 87 - 294 7e-42 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 161
AccI GTMKAC 2 cut(s) 731, 810
AccII CGCG 1 cut(s) 627
AciI CCGC 2 cut(s) 332, 446
AclWI GGATC 5 cut(s) 32, 499, 510, 883, 944
AcoI YGGCCR 1 cut(s) 483
AcsI RAATTY 3 cut(s) 47, 154, 932
AcuI CTGAAG 2 cut(s) 405, 585
AfaI GTAC 6 cut(s) 290, 299, 440, 545, 590, 1062
AfiI CCNNNNNNNGG 1 cut(s) 980
AflII CTTAAG 1 cut(s) 128
AgsI TTSAA 5 cut(s) 217, 247, 410, 634, 919
AjuI GAANNNNNNNTTGG 2 cut(s) 261, 293
Alw26I GTCTC 3 cut(s) 708, 860, 1022
AlwI GGATC 5 cut(s) 32, 499, 510, 883, 944
AoxI GGCC 2 cut(s) 74, 483
ApeKI GCWGC 8 cut(s) 6, 141, 230, 251, 529, 572, 1007, 1135
ApoI RAATTY 3 cut(s) 47, 154, 932
ArsI GACNNNNNNTTYG 2 cut(s) 95, 127
AseI ATTAAT 1 cut(s) 941
Asp700I GAANNNNTTC 1 cut(s) 1168
AspLEI GCGC 2 cut(s) 6, 162
AspS9I GGNCC 2 cut(s) 515, 704
AsuHPI GGTGA 4 cut(s) 497, 751, 835, 988
AvaII GGWCC 2 cut(s) 515, 704
BaeGI GKGCMC 2 cut(s) 766, 907
BalI TGGCCA 1 cut(s) 485
BbvI GCAGC 7 cut(s) 153, 217, 238, 541, 584, 1019, 1147
BccI CCATC 1 cut(s) 736
BcgI CGANNNNNNTGC 2 cut(s) 182, 216
BcoDI GTCTC 3 cut(s) 708, 860, 1022
BfaI CTAG 5 cut(s) 431, 512, 549, 893, 1184
BfmI CTRYAG 2 cut(s) 811, 1190
BfrI CTTAAG 1 cut(s) 128
BglII AGATCT 1 cut(s) 1186
BisI GCNGC 8 cut(s) 7, 142, 231, 252, 530, 573, 1008, 1136
BlsI GCNGC 8 cut(s) 8, 143, 232, 253, 531, 574, 1009, 1137
BmcAI AGTACT 1 cut(s) 590
Bme18I GGWCC 2 cut(s) 515, 704
BmgT120I GGNCC 2 cut(s) 515, 704
BmsI GCATC 4 cut(s) 137, 318, 647, 997
BoxI GACNNNNGTC 1 cut(s) 1145
BpmI CTGGAG 1 cut(s) 518
Bpu10I CCTNAGC 1 cut(s) 959
BpuEI CTTGAG 4 cut(s) 158, 605, 854, 1091
BsaWI WCCGGW 2 cut(s) 845, 973
Bsc4I CCNNNNNNNGG 1 cut(s) 980
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 1 cut(s) 486
BseGI GGATG 2 cut(s) 40, 558
BseLI CCNNNNNNNGG 1 cut(s) 980
BseMII CTCAG 1 cut(s) 228
BseNI ACTGG 1 cut(s) 486
BseSI GKGCMC 2 cut(s) 766, 907
BseXI GCAGC 7 cut(s) 153, 217, 238, 541, 584, 1019, 1147
BsgI GTGCAG 1 cut(s) 1154
Bsh1236I CGCG 1 cut(s) 627
BshFI GGCC 2 cut(s) 76, 485
BsiSI CCGG 3 cut(s) 26, 846, 974
BslFI GGGAC 1 cut(s) 706
BslI CCNNNNNNNGG 1 cut(s) 980
BsmAI GTCTC 3 cut(s) 708, 860, 1022
BsmFI GGGAC 1 cut(s) 706
BsmI GAATGC 1 cut(s) 363
BsnI GGCC 2 cut(s) 76, 485
Bsp1286I GDGCHC 2 cut(s) 766, 907
Bsp1407I TGTACA 1 cut(s) 288
Bsp143I GATC 6 cut(s) 37, 491, 502, 888, 949, 1186
Bsp68I TCGCGA 1 cut(s) 627
BspACI CCGC 2 cut(s) 332, 446
BspANI GGCC 2 cut(s) 76, 485
BspCNI CTCAG 1 cut(s) 229
BspFNI CGCG 1 cut(s) 627
BspHI TCATGA 1 cut(s) 523
BspPI GGATC 5 cut(s) 32, 499, 510, 883, 944
BspQI GCTCTTC 2 cut(s) 847, 876
BspTI CTTAAG 1 cut(s) 128
BsrFI RCCGGY 1 cut(s) 25
BsrGI TGTACA 1 cut(s) 288
BsrI ACTGG 1 cut(s) 486
BssAI RCCGGY 1 cut(s) 25
BssMI GATC 6 cut(s) 37, 491, 502, 888, 949, 1186
Bst4CI ACNGT 3 cut(s) 443, 815, 915
Bst6I CTCTTC 2 cut(s) 847, 876
BstAFI CTTAAG 1 cut(s) 128
BstAPI GCANNNNNTGC 1 cut(s) 147
BstAUI TGTACA 1 cut(s) 288
BstC8I GCNNGC 2 cut(s) 23, 778
BstDEI CTNAG 3 cut(s) 237, 696, 959
BstF5I GGATG 2 cut(s) 40, 558
BstFNI CGCG 1 cut(s) 627
BstHHI GCGC 2 cut(s) 6, 162
BstKTI GATC 6 cut(s) 40, 494, 505, 891, 952, 1189
BstMAI GTCTC 3 cut(s) 708, 860, 1022
BstMBI GATC 6 cut(s) 37, 491, 502, 888, 949, 1186
BstMWI GCNNNNNNNGC 4 cut(s) 147, 569, 611, 1007
BstPAI GACNNNNGTC 1 cut(s) 1145
BstSFI CTRYAG 2 cut(s) 811, 1190
BstSLI GKGCMC 2 cut(s) 766, 907
BstUI CGCG 1 cut(s) 627
BstV1I GCAGC 7 cut(s) 153, 217, 238, 541, 584, 1019, 1147
BstX2I RGATCY 3 cut(s) 491, 888, 1186
BstXI CCANNNNNNTGG 1 cut(s) 100
BstYI RGATCY 3 cut(s) 491, 888, 1186
BsuRI GGCC 2 cut(s) 76, 485
BtsCI GGATG 2 cut(s) 40, 558
BtsI GCAGTG 1 cut(s) 621
BtsIMutI CAGTG 3 cut(s) 493, 621, 911
BtuMI TCGCGA 1 cut(s) 627
Cac8I GCNNGC 2 cut(s) 23, 778
CciI TCATGA 1 cut(s) 523
CfoI GCGC 2 cut(s) 6, 162
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 2 cut(s) 515, 704
Csp6I GTAC 6 cut(s) 289, 298, 439, 544, 589, 1061
CviAII CATG 5 cut(s) 227, 394, 524, 596, 725
CviQI GTAC 6 cut(s) 289, 298, 439, 544, 589, 1061
DdeI CTNAG 3 cut(s) 237, 696, 959
DpnI GATC 6 cut(s) 39, 493, 504, 890, 951, 1188
DpnII GATC 6 cut(s) 37, 491, 502, 888, 949, 1186
EaeI YGGCCR 1 cut(s) 483
Eam1104I CTCTTC 2 cut(s) 847, 876
EarI CTCTTC 2 cut(s) 847, 876
Eco47I GGWCC 2 cut(s) 515, 704
Eco57I CTGAAG 2 cut(s) 405, 585
FaeI CATG 5 cut(s) 230, 397, 527, 599, 728
FaqI GGGAC 1 cut(s) 706
FatI CATG 5 cut(s) 226, 393, 523, 595, 724
FblI GTMKAC 2 cut(s) 731, 810
Fnu4HI GCNGC 8 cut(s) 7, 142, 231, 252, 530, 573, 1008, 1136
FokI GGATG 2 cut(s) 47, 565
Fsp4HI GCNGC 8 cut(s) 7, 142, 231, 252, 530, 573, 1008, 1136
FspBI CTAG 5 cut(s) 431, 512, 549, 893, 1184
FspI TGCGCA 1 cut(s) 161
GlaI GCGC 2 cut(s) 5, 161
GluI GCNGC 8 cut(s) 7, 142, 231, 252, 530, 573, 1008, 1136
GsuI CTGGAG 1 cut(s) 518
HaeIII GGCC 2 cut(s) 76, 485
HapII CCGG 3 cut(s) 26, 846, 974
HhaI GCGC 2 cut(s) 6, 162
Hin1II CATG 5 cut(s) 230, 397, 527, 599, 728
Hin6I GCGC 2 cut(s) 4, 160
HinP1I GCGC 2 cut(s) 4, 160
HinfI GANTC 5 cut(s) 104, 400, 520, 656, 1115
HpaII CCGG 3 cut(s) 26, 846, 974
HphI GGTGA 4 cut(s) 497, 751, 835, 988
Hpy166II GTNNAC 4 cut(s) 289, 546, 732, 811
Hpy188I TCNGA 9 cut(s) 45, 59, 168, 519, 672, 682, 805, 1054, 1174
Hpy188III TCNNGA 6 cut(s) 497, 524, 536, 626, 833, 1070
Hpy8I GTNNAC 4 cut(s) 289, 546, 732, 811
HpyAV CCTTC 9 cut(s) 66, 263, 277, 443, 518, 560, 913, 973, 1158
HpyCH4III ACNGT 3 cut(s) 443, 815, 915
HpyCH4IV ACGT 1 cut(s) 212
HpyF10VI GCNNNNNNNGC 4 cut(s) 147, 569, 611, 1007
HpyF3I CTNAG 3 cut(s) 237, 696, 959
HpySE526I ACGT 1 cut(s) 212
Hsp92II CATG 5 cut(s) 230, 397, 527, 599, 728
HspAI GCGC 2 cut(s) 4, 160
Kzo9I GATC 6 cut(s) 37, 491, 502, 888, 949, 1186
LguI GCTCTTC 2 cut(s) 847, 876
LmnI GCTCC 1 cut(s) 569
Lsp1109I GCAGC 7 cut(s) 153, 217, 238, 541, 584, 1019, 1147
LweI GCATC 4 cut(s) 137, 318, 647, 997
MaeI CTAG 5 cut(s) 431, 512, 549, 893, 1184
MaeII ACGT 1 cut(s) 212
MalI GATC 6 cut(s) 39, 493, 504, 890, 951, 1188
MboI GATC 6 cut(s) 37, 491, 502, 888, 949, 1186
MboII GAAGA 3 cut(s) 861, 864, 893
MfeI CAATTG 1 cut(s) 1002
MflI RGATCY 3 cut(s) 491, 888, 1186
MhlI GDGCHC 2 cut(s) 766, 907
MlsI TGGCCA 1 cut(s) 485
MluCI AATT 7 cut(s) 47, 154, 313, 662, 932, 942, 1002
MluNI TGGCCA 1 cut(s) 485
MlyI GAGTC 2 cut(s) 98, 409
MmeI TCCRAC 2 cut(s) 633, 731
MnlI CCTC 8 cut(s) 25, 51, 232, 266, 493, 543, 871, 1018
Mox20I TGGCCA 1 cut(s) 485
MroXI GAANNNNTTC 1 cut(s) 1168
MscI TGGCCA 1 cut(s) 485
MseI TTAA 5 cut(s) 129, 336, 345, 839, 941
MslI CAYNNNNRTG 2 cut(s) 551, 797
Msp20I TGGCCA 1 cut(s) 485
MspA1I CMGCKG 1 cut(s) 1138
MspCI CTTAAG 1 cut(s) 128
MspI CCGG 3 cut(s) 26, 846, 974
MunI CAATTG 1 cut(s) 1002
Mva1269I GAATGC 1 cut(s) 363
MvnI CGCG 1 cut(s) 627
MwoI GCNNNNNNNGC 4 cut(s) 147, 569, 611, 1007
NdeII GATC 6 cut(s) 37, 491, 502, 888, 949, 1186
NlaIII CATG 5 cut(s) 230, 397, 527, 599, 728
NmeAIII GCCGAG 1 cut(s) 181
NruI TCGCGA 1 cut(s) 627
NsbI TGCGCA 1 cut(s) 161
PagI TCATGA 1 cut(s) 523
PciSI GCTCTTC 2 cut(s) 847, 876
PctI GAATGC 1 cut(s) 363
PdmI GAANNNNTTC 1 cut(s) 1168
PfeI GAWTC 3 cut(s) 520, 656, 1115
PkrI GCNGC 8 cut(s) 8, 143, 232, 253, 531, 574, 1009, 1137
PleI GAGTC 2 cut(s) 98, 408
PpsI GAGTC 2 cut(s) 98, 408
PshAI GACNNNNGTC 1 cut(s) 1145
PshBI ATTAAT 1 cut(s) 941
PspPI GGNCC 2 cut(s) 515, 704
PsuI RGATCY 3 cut(s) 491, 888, 1186
PvuII CAGCTG 1 cut(s) 1138
RruI TCGCGA 1 cut(s) 627
RsaI GTAC 6 cut(s) 290, 299, 440, 545, 590, 1062
RsaNI GTAC 6 cut(s) 289, 298, 439, 544, 589, 1061
RseI CAYNNNNRTG 2 cut(s) 551, 797
SapI GCTCTTC 2 cut(s) 847, 876
SaqAI TTAA 5 cut(s) 129, 336, 345, 839, 941
SatI GCNGC 8 cut(s) 7, 142, 231, 252, 530, 573, 1008, 1136
Sau3AI GATC 6 cut(s) 37, 491, 502, 888, 949, 1186
Sau96I GGNCC 2 cut(s) 515, 704
ScaI AGTACT 1 cut(s) 590
SchI GAGTC 2 cut(s) 98, 409
SduI GDGCHC 2 cut(s) 766, 907
SfaNI GCATC 4 cut(s) 137, 318, 647, 997
SfcI CTRYAG 2 cut(s) 811, 1190
SinI GGWCC 2 cut(s) 515, 704
SmiMI CAYNNNNRTG 2 cut(s) 551, 797
SmlI CTYRAG 5 cut(s) 128, 173, 584, 833, 1070
SmoI CTYRAG 5 cut(s) 128, 173, 584, 833, 1070
Sse9I AATT 7 cut(s) 47, 154, 313, 662, 932, 942, 1002
SsiI CCGC 2 cut(s) 332, 446
SspMI CTAG 5 cut(s) 431, 512, 549, 893, 1184
TaaI ACNGT 3 cut(s) 443, 815, 915
TaiI ACGT 1 cut(s) 215
TaqI TCGA 1 cut(s) 861
TasI AATT 7 cut(s) 47, 154, 313, 662, 932, 942, 1002
TatI WGTACW 3 cut(s) 288, 588, 1060
TfiI GAWTC 3 cut(s) 520, 656, 1115
Tru1I TTAA 5 cut(s) 129, 336, 345, 839, 941
Tru9I TTAA 5 cut(s) 129, 336, 345, 839, 941
TscAI CASTG 3 cut(s) 493, 621, 918
TseI GCWGC 8 cut(s) 6, 141, 230, 251, 529, 572, 1007, 1135
TspDTI ATGAA 5 cut(s) 215, 489, 927, 1110, 1128
TspGWI ACGGA 1 cut(s) 942
TspRI CASTG 3 cut(s) 493, 621, 918
Vha464I CTTAAG 1 cut(s) 128
VpaK11BI GGWCC 2 cut(s) 515, 704
VspI ATTAAT 1 cut(s) 941
XapI RAATTY 3 cut(s) 47, 154, 932
XcmI CCANNNNNNNNNTGG 1 cut(s) 493
XmiI GTMKAC 2 cut(s) 731, 810
XmnI GAANNNNTTC 1 cut(s) 1168
XspI CTAG 5 cut(s) 431, 512, 549, 893, 1184
ZrmI AGTACT 1 cut(s) 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.