RLG00000032348

Protein phosphatase 2C 55

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0004533
Physical Location & Seq
Reverse (-)
13851389 .. 13852445
1057 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032348

Sequence Viewer

Length: 906 bp
ATGAATTGGCTCTCTAAGATACCTAGGGTTGCCGAAATCATTTGGGTACAGCTAGAGAGCGACCCAACTCCATTAAAAGAAGGAACCATACTGAAGATGGTCTGTGGGTCTTTTTATTTGCCCAAAGAAAGTGAAAATCGATCGCTACTAGGCGAAGACGCTCACTTCATTTGTGCAAAAGAGAACACCATTGGTGTTGCGGATGGTGTTGGCGGGTGGGTTAAGTATGGCGTGGATGCTGGAGAGTATGCAAGGAAGCTCATGGACAACTCAGTCATGGCTATACATAACCAACACAGAAAATCTAGAGATGCTGATGTCGATCCAATACAAATCTTGGATGAAGCCTACGGAAACACCAAGAATATTGCTGGGACATCAACAGCTTGCATCGTCACCCTTAGTAAAGAAGGTAGAACCTTGCATGCTGTGAATGTTGGCGATAGTGGTTTCATGCTGTTTAGAGACAAGAAGTGTGTGTACATTTCGCCAATTCGACAGAGTTACTTTAACTGTCCTCTTCAATTGGGGACAGGATCGGGAGATACTCCCCAATCGGCTACCGAGATTAAAGTTAGGGTACTTCGGCCGGGAGATGTCATAGTCCTTGGGACAGATGGGTTACTGGACAATGTGTCGCCTGGAGAAATAGAGAAAGTTCTGGAGGAGAATACCATAGAGGGTTTTGTGGAACCGGAGATGTTGGCATGGATCATAGCAGAGAGGCTGGCTTTACGCAACTCCGAGGATGCAGGAAGGTTGACTCCTTTTTCAATTGCGGCTCGTAAGGCCGGAGTCGAGCACATTGGAGGTAAGAGGGATGATATCACGGTCATTGTAGAGTCAGATCATGTATGTTGTGGTAGAGCCATTCATCATATGTTCCCCTGGCCTTCGAAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoIIE

Protein Analysis

302

Amino Acids

33.06

Weight (kDa)

5.55

Isoelectric Point (pI)

38.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C_2 PF13672 47 - 212 6.5e-06 Protein phosphatase 2C
SpoIIE PF07228 94 - 236 4.9e-07 Stage II sporulation protein E (SpoIIE)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 272
AciI CCGC 3 cut(s) 200, 213, 779
AclWI GGATC 3 cut(s) 317, 544, 719
AcoI YGGCCR 1 cut(s) 587
AcuI CTGAAG 1 cut(s) 113
AfaI GTAC 3 cut(s) 48, 482, 582
AgsI TTSAA 2 cut(s) 524, 774
AhdI GACNNNNNGTC 1 cut(s) 634
AjnI CCWGG 2 cut(s) 640, 887
AluBI AGCT 3 cut(s) 52, 259, 386
AluI AGCT 3 cut(s) 52, 259, 386
Alw21I GWGCWC 1 cut(s) 804
Alw26I GTCTC 1 cut(s) 459
AlwI GGATC 3 cut(s) 317, 544, 719
AoxI GGCC 3 cut(s) 587, 789, 890
AspA2I CCTAGG 1 cut(s) 23
AsuC2I CCSGG 1 cut(s) 591
AsuHPI GGTGA 1 cut(s) 388
AsuII TTCGAA 1 cut(s) 896
AvrII CCTAGG 1 cut(s) 23
BarI GAAGNNNNNNTAC 4 cut(s) 72, 104, 464, 496
BbsI GAAGAC 1 cut(s) 162
Bbv12I GWGCWC 1 cut(s) 804
BccI CCATC 3 cut(s) 91, 197, 611
BciT130I CCWGG 2 cut(s) 642, 889
BcnI CCSGG 1 cut(s) 591
BcoDI GTCTC 1 cut(s) 459
BfaI CTAG 4 cut(s) 24, 53, 149, 306
BisI GCNGC 1 cut(s) 780
BlnI CCTAGG 1 cut(s) 23
BlsI GCNGC 1 cut(s) 781
Bme1390I CCNGG 3 cut(s) 591, 642, 889
BmeRI GACNNNNNGTC 1 cut(s) 634
BmiI GGNNCC 2 cut(s) 85, 693
BmrFI CCNGG 3 cut(s) 591, 642, 889
BmsI GCATC 4 cut(s) 226, 301, 399, 739
BpiI GAAGAC 1 cut(s) 162
BpmI CTGGAG 3 cut(s) 261, 663, 683
Bpu14I TTCGAA 1 cut(s) 896
BpuMI CCSGG 1 cut(s) 591
Bsa29I ATCGAT 1 cut(s) 139
BsaBI GATNNNNATC 1 cut(s) 321
BsaJI CCNNGG 4 cut(s) 23, 607, 744, 887
BsaWI WCCGGW 1 cut(s) 694
Bse1I ACTGG 1 cut(s) 630
Bse8I GATNNNNATC 1 cut(s) 321
BseBI CCWGG 2 cut(s) 642, 889
BseCI ATCGAT 1 cut(s) 139
BseDI CCNNGG 4 cut(s) 23, 607, 744, 887
BseGI GGATG 5 cut(s) 208, 241, 346, 754, 826
BseJI GATNNNNATC 1 cut(s) 321
BseMII CTCAG 1 cut(s) 285
BseNI ACTGG 1 cut(s) 630
BseRI GAGGAG 1 cut(s) 680
BseX3I CGGCCG 1 cut(s) 587
BseYI CCCAGC 1 cut(s) 371
Bsh1285I CGRYCG 2 cut(s) 143, 590
BshFI GGCC 3 cut(s) 589, 791, 892
BshVI ATCGAT 1 cut(s) 139
BsiEI CGRYCG 2 cut(s) 143, 590
BsiHKAI GWGCWC 1 cut(s) 804
BsiSI CCGG 3 cut(s) 590, 695, 792
BslFI GGGAC 3 cut(s) 388, 544, 625
BsmAI GTCTC 1 cut(s) 459
BsmFI GGGAC 3 cut(s) 388, 544, 625
BsnI GGCC 3 cut(s) 589, 791, 892
Bsp119I TTCGAA 1 cut(s) 896
Bsp1286I GDGCHC 1 cut(s) 804
Bsp1407I TGTACA 1 cut(s) 480
Bsp143I GATC 5 cut(s) 140, 322, 536, 711, 847
BspACI CCGC 3 cut(s) 200, 213, 779
BspANI GGCC 3 cut(s) 589, 791, 892
BspCNI CTCAG 1 cut(s) 284
BspDI ATCGAT 1 cut(s) 139
BspLI GGNNCC 2 cut(s) 85, 693
BspPI GGATC 3 cut(s) 317, 544, 719
BspT104I TTCGAA 1 cut(s) 896
BsrGI TGTACA 1 cut(s) 480
BsrI ACTGG 1 cut(s) 630
BssECI CCNNGG 4 cut(s) 23, 607, 744, 887
BssMI GATC 5 cut(s) 140, 322, 536, 711, 847
BssT1I CCWWGG 2 cut(s) 23, 607
Bst2UI CCWGG 2 cut(s) 642, 889
Bst4CI ACNGT 2 cut(s) 515, 832
Bst6I CTCTTC 1 cut(s) 525
BstAUI TGTACA 1 cut(s) 480
BstBI TTCGAA 1 cut(s) 896
BstC8I GCNNGC 3 cut(s) 388, 426, 729
BstDEI CTNAG 3 cut(s) 15, 271, 401
BstF5I GGATG 5 cut(s) 208, 241, 346, 754, 826
BstKTI GATC 5 cut(s) 143, 325, 539, 714, 850
BstMAI GTCTC 1 cut(s) 459
BstMBI GATC 5 cut(s) 140, 322, 536, 711, 847
BstMCI CGRYCG 2 cut(s) 143, 590
BstMWI GCNNNNNNNGC 1 cut(s) 788
BstNI CCWGG 2 cut(s) 642, 889
BstNSI RCATGY 1 cut(s) 428
BstSCI CCNGG 3 cut(s) 589, 640, 887
BstV2I GAAGAC 1 cut(s) 162
BstZI CGGCCG 1 cut(s) 587
Bsu15I ATCGAT 1 cut(s) 139
BsuRI GGCC 3 cut(s) 589, 791, 892
BsuTUI ATCGAT 1 cut(s) 139
BtsCI GGATG 5 cut(s) 208, 241, 346, 754, 826
Cac8I GCNNGC 3 cut(s) 388, 426, 729
ClaI ATCGAT 1 cut(s) 139
CseI GACGC 1 cut(s) 167
Csp6I GTAC 3 cut(s) 47, 481, 581
CviAII CATG 6 cut(s) 262, 277, 425, 454, 708, 851
CviQI GTAC 3 cut(s) 47, 481, 581
DdeI CTNAG 3 cut(s) 15, 271, 401
DpnI GATC 5 cut(s) 142, 324, 538, 713, 849
DpnII GATC 5 cut(s) 140, 322, 536, 711, 847
DrdI GACNNNNNNGTC 1 cut(s) 272
DriI GACNNNNNGTC 1 cut(s) 634
DseDI GACNNNNNNGTC 1 cut(s) 272
EaeI YGGCCR 1 cut(s) 587
EagI CGGCCG 1 cut(s) 587
Eam1104I CTCTTC 1 cut(s) 525
Eam1105I GACNNNNNGTC 1 cut(s) 634
EarI CTCTTC 1 cut(s) 525
EclXI CGGCCG 1 cut(s) 587
Eco130I CCWWGG 2 cut(s) 23, 607
Eco32I GATATC 1 cut(s) 826
Eco52I CGGCCG 1 cut(s) 587
Eco57I CTGAAG 1 cut(s) 113
EcoRII CCWGG 2 cut(s) 640, 887
EcoRV GATATC 1 cut(s) 826
EcoT14I CCWWGG 2 cut(s) 23, 607
ErhI CCWWGG 2 cut(s) 23, 607
FaeI CATG 6 cut(s) 265, 280, 428, 457, 711, 854
FaqI GGGAC 3 cut(s) 388, 544, 625
FatI CATG 6 cut(s) 261, 276, 424, 453, 707, 850
FauI CCCGC 1 cut(s) 206
FauNDI CATATG 1 cut(s) 879
Fnu4HI GCNGC 1 cut(s) 780
FokI GGATG 5 cut(s) 215, 248, 353, 761, 833
Fsp4HI GCNGC 1 cut(s) 780
FspBI CTAG 4 cut(s) 24, 53, 149, 306
GluI GCNGC 1 cut(s) 780
GsaI CCCAGC 1 cut(s) 375
GsuI CTGGAG 3 cut(s) 261, 663, 683
HaeIII GGCC 3 cut(s) 589, 791, 892
HapII CCGG 3 cut(s) 590, 695, 792
HgaI GACGC 1 cut(s) 167
Hin1II CATG 6 cut(s) 265, 280, 428, 457, 711, 854
HincII GTYRAC 1 cut(s) 762
HindII GTYRAC 1 cut(s) 762
HinfI GANTC 3 cut(s) 763, 795, 842
HpaII CCGG 3 cut(s) 590, 695, 792
HphI GGTGA 1 cut(s) 388
Hpy166II GTNNAC 2 cut(s) 481, 762
Hpy188I TCNGA 2 cut(s) 745, 847
Hpy188III TCNNGA 3 cut(s) 306, 540, 662
Hpy8I GTNNAC 2 cut(s) 481, 762
HpyAV CCTTC 4 cut(s) 74, 404, 750, 903
HpyCH4III ACNGT 2 cut(s) 515, 832
HpyCH4V TGCA 5 cut(s) 176, 251, 390, 424, 752
HpyF10VI GCNNNNNNNGC 1 cut(s) 788
HpyF3I CTNAG 3 cut(s) 15, 271, 401
Hsp92II CATG 6 cut(s) 265, 280, 428, 457, 711, 854
Kzo9I GATC 5 cut(s) 140, 322, 536, 711, 847
LweI GCATC 4 cut(s) 226, 301, 399, 739
MaeI CTAG 4 cut(s) 24, 53, 149, 306
MaeIII GTNAC 3 cut(s) 394, 503, 621
MalI GATC 5 cut(s) 142, 324, 538, 713, 849
MboI GATC 5 cut(s) 140, 322, 536, 711, 847
MboII GAAGA 3 cut(s) 106, 167, 512
MfeI CAATTG 2 cut(s) 524, 774
MhlI GDGCHC 1 cut(s) 804
MluCI AATT 5 cut(s) 4, 492, 524, 774, 901
MlyI GAGTC 3 cut(s) 757, 804, 851
MnlI CCTC 7 cut(s) 528, 658, 673, 717, 739, 803, 810
MseI TTAA 4 cut(s) 74, 222, 510, 570
MspI CCGG 3 cut(s) 590, 695, 792
MspR9I CCNGG 3 cut(s) 591, 642, 889
MunI CAATTG 2 cut(s) 524, 774
MvaI CCWGG 2 cut(s) 642, 889
MwoI GCNNNNNNNGC 1 cut(s) 788
NciI CCSGG 1 cut(s) 591
NdeI CATATG 1 cut(s) 879
NdeII GATC 5 cut(s) 140, 322, 536, 711, 847
NlaIII CATG 6 cut(s) 265, 280, 428, 457, 711, 854
NlaIV GGNNCC 2 cut(s) 85, 693
NmuCI GTSAC 1 cut(s) 394
NspI RCATGY 1 cut(s) 428
NspV TTCGAA 1 cut(s) 896
PaeI GCATGC 1 cut(s) 428
PkrI GCNGC 1 cut(s) 781
Ple19I CGATCG 1 cut(s) 143
PleI GAGTC 3 cut(s) 757, 803, 850
PpsI GAGTC 3 cut(s) 757, 803, 850
Psp6I CCWGG 2 cut(s) 640, 887
PspFI CCCAGC 1 cut(s) 371
PspGI CCWGG 2 cut(s) 640, 887
PspN4I GGNNCC 2 cut(s) 85, 693
PvuI CGATCG 1 cut(s) 143
RsaI GTAC 3 cut(s) 48, 482, 582
RsaNI GTAC 3 cut(s) 47, 481, 581
SaqAI TTAA 4 cut(s) 74, 222, 510, 570
SatI GCNGC 1 cut(s) 780
Sau3AI GATC 5 cut(s) 140, 322, 536, 711, 847
SchI GAGTC 3 cut(s) 757, 804, 851
ScrFI CCNGG 3 cut(s) 591, 642, 889
SduI GDGCHC 1 cut(s) 804
SetI ASST 8 cut(s) 25, 54, 261, 388, 415, 422, 761, 814
SfaNI GCATC 4 cut(s) 226, 301, 399, 739
SfuI TTCGAA 1 cut(s) 896
SphI GCATGC 1 cut(s) 428
Sse9I AATT 5 cut(s) 4, 492, 524, 774, 901
SsiI CCGC 3 cut(s) 200, 213, 779
SspI AATATT 1 cut(s) 367
SspMI CTAG 4 cut(s) 24, 53, 149, 306
StyD4I CCNGG 3 cut(s) 589, 640, 887
StyI CCWWGG 2 cut(s) 23, 607
TaaI ACNGT 2 cut(s) 515, 832
TaqI TCGA 5 cut(s) 139, 321, 496, 798, 896
TasI AATT 5 cut(s) 4, 492, 524, 774, 901
TatI WGTACW 1 cut(s) 480
TauI GCSGC 1 cut(s) 782
Tru1I TTAA 4 cut(s) 74, 222, 510, 570
Tru9I TTAA 4 cut(s) 74, 222, 510, 570
TseFI GTSAC 1 cut(s) 394
Tsp45I GTSAC 1 cut(s) 394
TspDTI ATGAA 5 cut(s) 17, 157, 357, 442, 863
TspGWI ACGGA 1 cut(s) 366
XbaI TCTAGA 1 cut(s) 305
XceI RCATGY 1 cut(s) 428
XcmI CCANNNNNNNNNTGG 1 cut(s) 94
XmaJI CCTAGG 1 cut(s) 23
XspI CTAG 4 cut(s) 24, 53, 149, 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.