MD11G1261400.v1.1

protein phosphatase 2C 55

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0004533
Physical Location & Seq
Reverse (-)
37629162 .. 37630193
1032 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1261400.v1.1.491

Sequence Viewer

Length: 1032 bp
ATGCTGATTGCTAAGAAGCCCGGTAGGGTTGAAGACCCCCGCTTCGTCTTCCCGGAGAAGCAAACTATCTGCGTGGCGGACGGTGTCGGTTGGGCCAAGACAGACGTCGACGCCGGTGACTATGCAGGGGAACAGATGATGCTGACGGAAGCTACTTTGAGTTTATTCATGTTGAAGTACGTAAACCACAATGGTATCATCAACCACAACAATAGCTATGGTCAAGAAACAAAGAGAAACCTGCACGAGATGGTTTGTGGGTCGGTGTATCTTGCCAAAGATAACAAGTTAGGCGAAGACGCTCACTTCATCTGCCCGGAGAAGCTGACTATCGGCGTGGCAGATGGCGTTGGCGGTTGGGCTAACAAAGGCATCGACGCTGGTGAGTACGCGAGGGAACTCATGAACAATGCCTTTATCGCAGTCCATAATCCGAATAACATCAACGAACAGAAAAGAAAAAGAAAGGCCGCGGATATGATCGGAGCCGTTGATCCAAGAAAAGTGTTGCAGGAGGCTTATGCAAAGACCAAGGAGAAAGGCTCGTCGACCGCTTGCATCCTCAGCCTTAACAAAGAGAGCGGAGTCTTGCATGCTGTGAACGTTGGGGACAGTGGGTTCTTGCTATTTAGGAACAACAAGGTTGTGTACCAATCTCCAACTCAGCAACGCAGGTTCAACTGTCCCTATCAGTTGGGGAACAGCATCAGCAGCGACCGCCCTGACTGCGCTTGGGAGGAGTGGATTCAGACAGTCCCCGGAGACATGCTAGTGCTTGGGACTGATGGCTTGCTGGACAACATGTTTCCAAGTGAGATTGAGAAGGTTCTTGTACAAGGTGGTGGTGGTGGTGATCTTGATCTTGATTGTGGCACGTTGGCTTCGAGCATCGCCAATCTGGCTCTCTATAACTCGTTCGATAAGTACAACCCTAGCCCTTTTTCCGAAAATGCTAGAAAGGCTGGATTCGAGCACAAAGGAGGCAAGATCGACGATATTACTGTTATTGTTGCTCAAGTTGTGGCTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoIIE

Protein Analysis

344

Amino Acids

37.46

Weight (kDa)

5.58

Isoelectric Point (pI)

38.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoIIE PF07228 147 - 268 9.3e-06 Stage II sporulation protein E (SpoIIE)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 108
Acc36I ACCTGC 2 cut(s) 249, 663
AccBSI CCGCTC 1 cut(s) 582
AccI GTMKAC 2 cut(s) 108, 548
AccII CGCG 2 cut(s) 392, 473
AciI CCGC 8 cut(s) 40, 77, 354, 471, 473, 552, 582, 718
AclI AACGTT 1 cut(s) 603
AclWI GGATC 1 cut(s) 488
AcyI GRCGYC 2 cut(s) 105, 111
AfaI GTAC 5 cut(s) 179, 389, 650, 834, 926
AflIII ACRYGT 1 cut(s) 801
AgsI TTSAA 3 cut(s) 32, 175, 679
AloI GAACNNNNNNTCC 2 cut(s) 602, 634
AluBI AGCT 3 cut(s) 152, 216, 325
AluI AGCT 3 cut(s) 152, 216, 325
Alw21I GWGCWC 1 cut(s) 975
Alw26I GTCTC 1 cut(s) 756
AlwI GGATC 1 cut(s) 488
AoxI GGCC 2 cut(s) 93, 468
ApeKI GCWGC 1 cut(s) 711
ArsI GACNNNNNNTTYG 2 cut(s) 26, 58
AspLEI GCGC 1 cut(s) 731
AspS9I GGNCC 1 cut(s) 93
AsuC2I CCSGG 4 cut(s) 21, 53, 317, 759
AsuHPI GGTGA 3 cut(s) 128, 395, 863
BaeI ACNNNNGTAYC 2 cut(s) 178, 211
BauI CACGAG 1 cut(s) 245
BbsI GAAGAC 3 cut(s) 39, 40, 303
Bbv12I GWGCWC 1 cut(s) 975
BbvCI CCTCAGC 1 cut(s) 563
BbvI GCAGC 1 cut(s) 723
BccI CCATC 3 cut(s) 244, 338, 779
BceAI ACGGC 1 cut(s) 473
BcnI CCSGG 4 cut(s) 21, 53, 317, 759
BcoDI GTCTC 1 cut(s) 756
BfaI CTAG 3 cut(s) 770, 933, 954
BfuAI ACCTGC 2 cut(s) 249, 663
BglI GCCNNNNNGGC 1 cut(s) 899
BisI GCNGC 2 cut(s) 471, 712
BlsI GCNGC 2 cut(s) 472, 713
Bme1390I CCNGG 4 cut(s) 21, 53, 317, 759
BmgT120I GGNCC 1 cut(s) 93
BmiI GGNNCC 1 cut(s) 487
BmrFI CCNGG 4 cut(s) 21, 53, 317, 759
BmsI GCATC 5 cut(s) 129, 381, 567, 714, 897
BoxI GACNNNNGTC 1 cut(s) 104
BpiI GAAGAC 3 cut(s) 39, 40, 303
BplI GAGNNNNNCTC 2 cut(s) 527, 559
Bpu10I CCTNAGC 1 cut(s) 563
BpuEI CTTGAG 1 cut(s) 999
BpuMI CCSGG 4 cut(s) 21, 53, 317, 759
BsaAI YACGTR 1 cut(s) 181
BsaBI GATNNNNATC 1 cut(s) 858
BsaHI GRCGYC 2 cut(s) 105, 111
BsaJI CCNNGG 3 cut(s) 471, 531, 757
Bse118I RCCGGY 1 cut(s) 113
Bse8I GATNNNNATC 1 cut(s) 858
BseDI CCNNGG 3 cut(s) 471, 531, 757
BseGI GGATG 1 cut(s) 558
BseJI GATNNNNATC 1 cut(s) 858
BseMII CTCAG 2 cut(s) 577, 677
BseRI GAGGAG 1 cut(s) 752
BseXI GCAGC 1 cut(s) 723
BsgI GTGCAG 1 cut(s) 227
Bsh1236I CGCG 2 cut(s) 392, 473
Bsh1285I CGRYCG 2 cut(s) 552, 718
BshFI GGCC 2 cut(s) 95, 470
BsiEI CGRYCG 2 cut(s) 552, 718
BsiHKAI GWGCWC 1 cut(s) 975
BsiSI CCGG 5 cut(s) 21, 53, 114, 317, 759
BslFI GGGAC 4 cut(s) 623, 669, 740, 793
BsmAI GTCTC 1 cut(s) 756
BsmFI GGGAC 4 cut(s) 623, 669, 740, 793
BsnI GGCC 2 cut(s) 95, 470
Bsp1286I GDGCHC 1 cut(s) 975
Bsp1407I TGTACA 1 cut(s) 832
Bsp143I GATC 5 cut(s) 480, 493, 853, 859, 987
BspACI CCGC 8 cut(s) 40, 77, 354, 471, 473, 552, 582, 718
BspANI GGCC 2 cut(s) 95, 470
BspCNI CTCAG 2 cut(s) 576, 676
BspFNI CGCG 2 cut(s) 392, 473
BspHI TCATGA 1 cut(s) 402
BspLI GGNNCC 1 cut(s) 487
BspMI ACCTGC 2 cut(s) 249, 663
BspPI GGATC 1 cut(s) 488
BsrBI CCGCTC 1 cut(s) 582
BsrFI RCCGGY 1 cut(s) 113
BsrGI TGTACA 1 cut(s) 832
BssAI RCCGGY 1 cut(s) 113
BssECI CCNNGG 3 cut(s) 471, 531, 757
BssMI GATC 5 cut(s) 480, 493, 853, 859, 987
BssNI GRCGYC 2 cut(s) 105, 111
BssSI CACGAG 1 cut(s) 245
BssT1I CCWWGG 1 cut(s) 531
Bst2BI CACGAG 1 cut(s) 245
Bst4CI ACNGT 5 cut(s) 83, 614, 683, 754, 1003
BstACI GRCGYC 2 cut(s) 105, 111
BstAUI TGTACA 1 cut(s) 832
BstBAI YACGTR 1 cut(s) 181
BstC8I GCNNGC 3 cut(s) 556, 594, 791
BstDEI CTNAG 3 cut(s) 12, 563, 663
BstDSI CCRYGG 1 cut(s) 471
BstF5I GGATG 1 cut(s) 558
BstFNI CGCG 2 cut(s) 392, 473
BstHHI GCGC 1 cut(s) 731
BstKTI GATC 5 cut(s) 483, 496, 856, 862, 990
BstMAI GTCTC 1 cut(s) 756
BstMBI GATC 5 cut(s) 480, 493, 853, 859, 987
BstMCI CGRYCG 2 cut(s) 552, 718
BstMWI GCNNNNNNNGC 7 cut(s) 419, 564, 711, 717, 726, 899, 959
BstNSI RCATGY 3 cut(s) 596, 769, 805
BstPAI GACNNNNGTC 1 cut(s) 104
BstSCI CCNGG 4 cut(s) 19, 51, 315, 757
BstSNI TACGTA 1 cut(s) 181
BstUI CGCG 2 cut(s) 392, 473
BstV1I GCAGC 1 cut(s) 723
BstV2I GAAGAC 3 cut(s) 39, 40, 303
BsuRI GGCC 2 cut(s) 95, 470
BtgI CCRYGG 1 cut(s) 471
BtgZI GCGATG 1 cut(s) 874
BtsCI GGATG 1 cut(s) 558
BtsIMutI CAGTG 1 cut(s) 619
BveI ACCTGC 2 cut(s) 249, 663
Cac8I GCNNGC 3 cut(s) 556, 594, 791
CciI TCATGA 1 cut(s) 402
CfoI GCGC 1 cut(s) 731
Cfr10I RCCGGY 1 cut(s) 113
Cfr13I GGNCC 1 cut(s) 93
Cfr42I CCGCGG 1 cut(s) 474
CseI GACGC 3 cut(s) 119, 308, 386
Csp6I GTAC 5 cut(s) 178, 388, 649, 833, 925
CviAII CATG 5 cut(s) 169, 403, 593, 766, 802
CviQI GTAC 5 cut(s) 178, 388, 649, 833, 925
DdeI CTNAG 3 cut(s) 12, 563, 663
DpnI GATC 5 cut(s) 482, 495, 855, 861, 989
DpnII GATC 5 cut(s) 480, 493, 853, 859, 987
EciI GGCGGA 1 cut(s) 92
Eco105I TACGTA 1 cut(s) 181
Eco130I CCWWGG 1 cut(s) 531
EcoT14I CCWWGG 1 cut(s) 531
ErhI CCWWGG 1 cut(s) 531
FaeI CATG 5 cut(s) 172, 406, 596, 769, 805
FaqI GGGAC 4 cut(s) 623, 669, 740, 793
FatI CATG 5 cut(s) 168, 402, 592, 765, 801
FauI CCCGC 1 cut(s) 47
FblI GTMKAC 2 cut(s) 108, 548
Fnu4HI GCNGC 2 cut(s) 471, 712
FokI GGATG 1 cut(s) 545
Fsp4HI GCNGC 2 cut(s) 471, 712
FspBI CTAG 3 cut(s) 770, 933, 954
GlaI GCGC 1 cut(s) 730
GluI GCNGC 2 cut(s) 471, 712
HaeIII GGCC 2 cut(s) 95, 470
HapII CCGG 5 cut(s) 21, 53, 114, 317, 759
HgaI GACGC 3 cut(s) 119, 308, 386
HhaI GCGC 1 cut(s) 731
Hin1I GRCGYC 2 cut(s) 105, 111
Hin1II CATG 5 cut(s) 172, 406, 596, 769, 805
Hin6I GCGC 1 cut(s) 729
HinP1I GCGC 1 cut(s) 729
HincII GTYRAC 2 cut(s) 109, 549
HindII GTYRAC 2 cut(s) 109, 549
HinfI GANTC 3 cut(s) 585, 745, 966
HpaII CCGG 5 cut(s) 21, 53, 114, 317, 759
HphI GGTGA 3 cut(s) 128, 395, 863
Hpy166II GTNNAC 5 cut(s) 109, 184, 549, 601, 649
Hpy188I TCNGA 4 cut(s) 435, 485, 750, 946
Hpy188III TCNNGA 4 cut(s) 224, 403, 857, 863
Hpy8I GTNNAC 5 cut(s) 109, 184, 549, 601, 649
Hpy99I CGWCG 5 cut(s) 110, 113, 380, 550, 995
HpyAV CCTTC 1 cut(s) 817
HpyCH4III ACNGT 5 cut(s) 83, 614, 683, 754, 1003
HpyCH4IV ACGT 4 cut(s) 105, 180, 603, 875
HpyCH4V TGCA 6 cut(s) 125, 244, 511, 524, 558, 592
HpyF10VI GCNNNNNNNGC 7 cut(s) 419, 564, 711, 717, 726, 899, 959
HpyF3I CTNAG 3 cut(s) 12, 563, 663
HpySE526I ACGT 4 cut(s) 105, 180, 603, 875
Hsp92I GRCGYC 2 cut(s) 105, 111
Hsp92II CATG 5 cut(s) 172, 406, 596, 769, 805
HspAI GCGC 1 cut(s) 729
KspI CCGCGG 1 cut(s) 474
Kzo9I GATC 5 cut(s) 480, 493, 853, 859, 987
LmnI GCTCC 1 cut(s) 485
Lsp1109I GCAGC 1 cut(s) 723
LweI GCATC 5 cut(s) 129, 381, 567, 714, 897
MaeI CTAG 3 cut(s) 770, 933, 954
MaeII ACGT 4 cut(s) 105, 180, 603, 875
MaeIII GTNAC 1 cut(s) 116
MalI GATC 5 cut(s) 482, 495, 855, 861, 989
MbiI CCGCTC 1 cut(s) 582
MboI GATC 5 cut(s) 480, 493, 853, 859, 987
MboII GAAGA 3 cut(s) 40, 44, 308
MhlI GDGCHC 1 cut(s) 975
MlyI GAGTC 1 cut(s) 594
MmeI TCCRAC 1 cut(s) 683
MnlI CCTC 5 cut(s) 387, 508, 572, 730, 974
MseI TTAA 1 cut(s) 570
MslI CAYNNNNRTG 1 cut(s) 770
MspA1I CMGCKG 1 cut(s) 473
MspI CCGG 5 cut(s) 21, 53, 114, 317, 759
MspR9I CCNGG 4 cut(s) 21, 53, 317, 759
MvnI CGCG 2 cut(s) 392, 473
MwoI GCNNNNNNNGC 7 cut(s) 419, 564, 711, 717, 726, 899, 959
NciI CCSGG 4 cut(s) 21, 53, 317, 759
NdeII GATC 5 cut(s) 480, 493, 853, 859, 987
NlaIII CATG 5 cut(s) 172, 406, 596, 769, 805
NlaIV GGNNCC 1 cut(s) 487
NmuCI GTSAC 1 cut(s) 116
NspI RCATGY 3 cut(s) 596, 769, 805
PaeI GCATGC 1 cut(s) 596
PagI TCATGA 1 cut(s) 402
PciI ACATGT 1 cut(s) 801
PcsI WCGNNNNNNNCGW 1 cut(s) 881
PfeI GAWTC 2 cut(s) 745, 966
PflFI GACNNNGTC 1 cut(s) 83
PfoI TCCNGGA 1 cut(s) 51
PkrI GCNGC 2 cut(s) 472, 713
PleI GAGTC 1 cut(s) 593
PpsI GAGTC 1 cut(s) 593
Ppu21I YACGTR 1 cut(s) 181
PscI ACATGT 1 cut(s) 801
PshAI GACNNNNGTC 1 cut(s) 104
Psp1406I AACGTT 1 cut(s) 603
PspN4I GGNNCC 1 cut(s) 487
PspPI GGNCC 1 cut(s) 93
PsyI GACNNNGTC 1 cut(s) 83
RsaI GTAC 5 cut(s) 179, 389, 650, 834, 926
RsaNI GTAC 5 cut(s) 178, 388, 649, 833, 925
RseI CAYNNNNRTG 1 cut(s) 770
SacII CCGCGG 1 cut(s) 474
SalI GTCGAC 2 cut(s) 107, 547
SaqAI TTAA 1 cut(s) 570
SatI GCNGC 2 cut(s) 471, 712
Sau3AI GATC 5 cut(s) 480, 493, 853, 859, 987
Sau96I GGNCC 1 cut(s) 93
SchI GAGTC 1 cut(s) 594
ScrFI CCNGG 4 cut(s) 21, 53, 317, 759
SduI GDGCHC 1 cut(s) 975
SfaNI GCATC 5 cut(s) 129, 381, 567, 714, 897
Sfr303I CCGCGG 1 cut(s) 474
SgrAI CRCCGGYG 1 cut(s) 113
SgrBI CCGCGG 1 cut(s) 474
SgrDI CGTCGACG 1 cut(s) 107
SmiMI CAYNNNNRTG 1 cut(s) 770
SmlI CTYRAG 1 cut(s) 1014
SmoI CTYRAG 1 cut(s) 1014
SnaBI TACGTA 1 cut(s) 181
SphI GCATGC 1 cut(s) 596
SsiI CCGC 8 cut(s) 40, 77, 354, 471, 473, 552, 582, 718
SspMI CTAG 3 cut(s) 770, 933, 954
StyD4I CCNGG 4 cut(s) 19, 51, 315, 757
StyI CCWWGG 1 cut(s) 531
TaaI ACNGT 5 cut(s) 83, 614, 683, 754, 1003
TaiI ACGT 4 cut(s) 108, 183, 606, 878
TaqI TCGA 7 cut(s) 108, 375, 548, 884, 918, 969, 990
TatI WGTACW 2 cut(s) 832, 924
TauI GCSGC 1 cut(s) 473
TfiI GAWTC 2 cut(s) 745, 966
Tru1I TTAA 1 cut(s) 570
Tru9I TTAA 1 cut(s) 570
TscAI CASTG 1 cut(s) 619
TseFI GTSAC 1 cut(s) 116
TseI GCWGC 1 cut(s) 711
Tsp45I GTSAC 1 cut(s) 116
TspDTI ATGAA 3 cut(s) 157, 298, 419
TspGWI ACGGA 1 cut(s) 161
TspRI CASTG 1 cut(s) 619
Tth111I GACNNNGTC 1 cut(s) 83
XceI RCATGY 3 cut(s) 596, 769, 805
XmiI GTMKAC 2 cut(s) 108, 548
XspI CTAG 3 cut(s) 770, 933, 954
ZraI GACGTC 1 cut(s) 106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.