Rh5BG132700

Protein phosphatase 2C 55

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0004533
Physical Location & Seq
Reverse (-)
13140168 .. 13140851
684 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG132700.1

Sequence Viewer

Length: 684 bp
ATGAATTGGCTCTCGAAGATACCTAGGGTTATGAATTGGGTCTCGAAGATACCTAGGGTTGCCGAAATCATTTGGGTACAGCTAGAGAGCGACCCAACTCCATTAAAAGAAGGAACCATACTGAAGATGGTCTGTGGGTCTTTTTATTTGCCCAAAGAAAGTGAAAATCGATCACTTCTAGGCGAAGACGCTCACTTCATTTGTGCAGAAGAGAACACTATTGGTGTCGCGGATGGTGTTGGCGGGTGGGCTAAGTATGGCGTGGATGCTGGAGAGTATGCAAGGAAGCTCATGGACAACTCAGTCATGGCTGTACATAACCAACATAGAAAATCTGGAGATGTTGATGTCGATCCGATACAAATTTTGCATGAAGCCTACGGAAACACCAAGAATATTGCTGGGACATCAACAGCTTGCATCGTCACGCTTAGTGACGAAGGTAGAACCTTGCATGCTGTGAATGTTGGGGATAGTGGTTTCATGCTGTTTAGAGACAAGAAGTGTGTGTACATTTCGCCAATTCGACAGGTTTACTTTAACTGTCCTCTTCAATTGGGGACAGGATCAGGAAATACTCCCCAATCAGCTACCGAGATTAAAGTTAAGGTACTTCGGCCGGGAGATGTCATAGTTCTTGGGACAGATGGGTTACTGGACAATGTGTCGCCGGGAGAAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoIIE

Protein Analysis

227

Amino Acids

24.76

Weight (kDa)

5.55

Isoelectric Point (pI)

26.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoIIE PF07228 110 - 221 5.1e-06 Stage II sporulation protein E (SpoIIE)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 302
AccII CGCG 1 cut(s) 230
AciI CCGC 2 cut(s) 230, 243
AclWI GGATC 2 cut(s) 347, 574
AcoI YGGCCR 1 cut(s) 617
AcsI RAATTY 1 cut(s) 363
AcuI CTGAAG 1 cut(s) 143
AfaI GTAC 4 cut(s) 78, 315, 512, 612
AgsI TTSAA 1 cut(s) 554
AhdI GACNNNNNGTC 1 cut(s) 664
AluBI AGCT 4 cut(s) 82, 289, 416, 590
AluI AGCT 4 cut(s) 82, 289, 416, 590
Alw26I GTCTC 2 cut(s) 46, 489
AlwI GGATC 2 cut(s) 347, 574
AoxI GGCC 1 cut(s) 617
ApoI RAATTY 1 cut(s) 363
AspA2I CCTAGG 2 cut(s) 23, 53
AsuC2I CCSGG 2 cut(s) 621, 672
AvrII CCTAGG 2 cut(s) 23, 53
BarI GAAGNNNNNNTAC 4 cut(s) 102, 134, 494, 526
BbsI GAAGAC 1 cut(s) 192
BccI CCATC 3 cut(s) 121, 227, 641
BcnI CCSGG 2 cut(s) 621, 672
BcoDI GTCTC 2 cut(s) 46, 489
BfaI CTAG 4 cut(s) 24, 54, 83, 179
BlnI CCTAGG 2 cut(s) 23, 53
Bme1390I CCNGG 2 cut(s) 621, 672
BmeRI GACNNNNNGTC 1 cut(s) 664
BmiI GGNNCC 1 cut(s) 115
BmrFI CCNGG 2 cut(s) 621, 672
BmsI GCATC 2 cut(s) 256, 429
BpiI GAAGAC 1 cut(s) 192
BpmI CTGGAG 2 cut(s) 291, 357
BpuMI CCSGG 2 cut(s) 621, 672
Bsa29I ATCGAT 1 cut(s) 169
BsaBI GATNNNNATC 1 cut(s) 351
BsaI GGTCTC 1 cut(s) 46
BsaJI CCNNGG 2 cut(s) 23, 53
Bse1I ACTGG 1 cut(s) 660
Bse8I GATNNNNATC 1 cut(s) 351
BseCI ATCGAT 1 cut(s) 169
BseDI CCNNGG 2 cut(s) 23, 53
BseGI GGATG 2 cut(s) 238, 271
BseJI GATNNNNATC 1 cut(s) 351
BseMII CTCAG 1 cut(s) 315
BseNI ACTGG 1 cut(s) 660
BseX3I CGGCCG 1 cut(s) 617
BseYI CCCAGC 1 cut(s) 401
BsgI GTGCAG 1 cut(s) 225
Bsh1236I CGCG 1 cut(s) 230
Bsh1285I CGRYCG 1 cut(s) 620
BshFI GGCC 1 cut(s) 619
BshVI ATCGAT 1 cut(s) 169
BsiEI CGRYCG 1 cut(s) 620
BsiSI CCGG 2 cut(s) 620, 671
BslFI GGGAC 3 cut(s) 418, 574, 655
BsmAI GTCTC 2 cut(s) 46, 489
BsmFI GGGAC 3 cut(s) 418, 574, 655
BsnI GGCC 1 cut(s) 619
Bso31I GGTCTC 1 cut(s) 46
Bsp1407I TGTACA 2 cut(s) 313, 510
Bsp143I GATC 3 cut(s) 170, 352, 566
BspACI CCGC 2 cut(s) 230, 243
BspANI GGCC 1 cut(s) 619
BspCNI CTCAG 1 cut(s) 314
BspDI ATCGAT 1 cut(s) 169
BspFNI CGCG 1 cut(s) 230
BspLI GGNNCC 1 cut(s) 115
BspPI GGATC 2 cut(s) 347, 574
BspTNI GGTCTC 1 cut(s) 46
BsrGI TGTACA 2 cut(s) 313, 510
BsrI ACTGG 1 cut(s) 660
BssECI CCNNGG 2 cut(s) 23, 53
BssMI GATC 3 cut(s) 170, 352, 566
BssT1I CCWWGG 2 cut(s) 23, 53
Bst4CI ACNGT 1 cut(s) 545
Bst6I CTCTTC 2 cut(s) 204, 555
BstAUI TGTACA 2 cut(s) 313, 510
BstC8I GCNNGC 2 cut(s) 418, 456
BstDEI CTNAG 3 cut(s) 252, 301, 431
BstF5I GGATG 2 cut(s) 238, 271
BstFNI CGCG 1 cut(s) 230
BstKTI GATC 3 cut(s) 173, 355, 569
BstMAI GTCTC 2 cut(s) 46, 489
BstMBI GATC 3 cut(s) 170, 352, 566
BstMCI CGRYCG 1 cut(s) 620
BstNSI RCATGY 1 cut(s) 458
BstSCI CCNGG 2 cut(s) 619, 670
BstUI CGCG 1 cut(s) 230
BstV2I GAAGAC 1 cut(s) 192
BstZI CGGCCG 1 cut(s) 617
Bsu15I ATCGAT 1 cut(s) 169
BsuRI GGCC 1 cut(s) 619
BsuTUI ATCGAT 1 cut(s) 169
BtsCI GGATG 2 cut(s) 238, 271
Cac8I GCNNGC 2 cut(s) 418, 456
ClaI ATCGAT 1 cut(s) 169
CseI GACGC 1 cut(s) 197
Csp6I GTAC 4 cut(s) 77, 314, 511, 611
CviAII CATG 5 cut(s) 292, 307, 371, 455, 484
CviJI RGCY 9 cut(s) 10, 82, 251, 289, 311, 377, 416, 590, 619
CviKI_1 RGCY 9 cut(s) 10, 82, 251, 289, 311, 377, 416, 590, 619
CviQI GTAC 4 cut(s) 77, 314, 511, 611
DdeI CTNAG 3 cut(s) 252, 301, 431
DpnI GATC 3 cut(s) 172, 354, 568
DpnII GATC 3 cut(s) 170, 352, 566
DrdI GACNNNNNNGTC 1 cut(s) 302
DriI GACNNNNNGTC 1 cut(s) 664
DseDI GACNNNNNNGTC 1 cut(s) 302
EaeI YGGCCR 1 cut(s) 617
EagI CGGCCG 1 cut(s) 617
Eam1104I CTCTTC 2 cut(s) 204, 555
Eam1105I GACNNNNNGTC 1 cut(s) 664
EarI CTCTTC 2 cut(s) 204, 555
EclXI CGGCCG 1 cut(s) 617
Eco130I CCWWGG 2 cut(s) 23, 53
Eco31I GGTCTC 1 cut(s) 46
Eco52I CGGCCG 1 cut(s) 617
Eco57I CTGAAG 1 cut(s) 143
EcoT14I CCWWGG 2 cut(s) 23, 53
ErhI CCWWGG 2 cut(s) 23, 53
FaeI CATG 5 cut(s) 295, 310, 374, 458, 487
FaqI GGGAC 3 cut(s) 418, 574, 655
FatI CATG 5 cut(s) 291, 306, 370, 454, 483
FauI CCCGC 1 cut(s) 236
FokI GGATG 2 cut(s) 245, 278
FspBI CTAG 4 cut(s) 24, 54, 83, 179
GsaI CCCAGC 1 cut(s) 405
GsuI CTGGAG 2 cut(s) 291, 357
HaeIII GGCC 1 cut(s) 619
HapII CCGG 2 cut(s) 620, 671
HgaI GACGC 1 cut(s) 197
Hin1II CATG 5 cut(s) 295, 310, 374, 458, 487
HpaII CCGG 2 cut(s) 620, 671
Hpy166II GTNNAC 2 cut(s) 511, 535
Hpy188I TCNGA 1 cut(s) 357
Hpy188III TCNNGA 4 cut(s) 13, 43, 336, 570
Hpy8I GTNNAC 2 cut(s) 511, 535
HpyAV CCTTC 2 cut(s) 104, 434
HpyCH4III ACNGT 1 cut(s) 545
HpyCH4V TGCA 5 cut(s) 206, 281, 370, 420, 454
HpyF3I CTNAG 3 cut(s) 252, 301, 431
Hsp92II CATG 5 cut(s) 295, 310, 374, 458, 487
Kzo9I GATC 3 cut(s) 170, 352, 566
LpnPI CCDG 8 cut(s) 255, 321, 387, 515, 549, 555, 633, 641
LweI GCATC 2 cut(s) 256, 429
MaeI CTAG 4 cut(s) 24, 54, 83, 179
MaeIII GTNAC 3 cut(s) 424, 434, 651
MalI GATC 3 cut(s) 172, 354, 568
MboI GATC 3 cut(s) 170, 352, 566
MboII GAAGA 6 cut(s) 28, 58, 136, 197, 221, 542
MfeI CAATTG 1 cut(s) 554
MluCI AATT 5 cut(s) 4, 34, 363, 522, 554
MnlI CCTC 1 cut(s) 558
MseI TTAA 4 cut(s) 104, 540, 600, 606
MspI CCGG 2 cut(s) 620, 671
MspR9I CCNGG 2 cut(s) 621, 672
MunI CAATTG 1 cut(s) 554
MvnI CGCG 1 cut(s) 230
NciI CCSGG 2 cut(s) 621, 672
NdeII GATC 3 cut(s) 170, 352, 566
NlaIII CATG 5 cut(s) 295, 310, 374, 458, 487
NlaIV GGNNCC 1 cut(s) 115
NmuCI GTSAC 2 cut(s) 424, 434
NspI RCATGY 1 cut(s) 458
PaeI GCATGC 1 cut(s) 458
PspFI CCCAGC 1 cut(s) 401
PspN4I GGNNCC 1 cut(s) 115
RsaI GTAC 4 cut(s) 78, 315, 512, 612
RsaNI GTAC 4 cut(s) 77, 314, 511, 611
SaqAI TTAA 4 cut(s) 104, 540, 600, 606
Sau3AI GATC 3 cut(s) 170, 352, 566
ScrFI CCNGG 2 cut(s) 621, 672
SfaNI GCATC 2 cut(s) 256, 429
SphI GCATGC 1 cut(s) 458
Sse9I AATT 5 cut(s) 4, 34, 363, 522, 554
SsiI CCGC 2 cut(s) 230, 243
SspI AATATT 1 cut(s) 397
SspMI CTAG 4 cut(s) 24, 54, 83, 179
StyD4I CCNGG 2 cut(s) 619, 670
StyI CCWWGG 2 cut(s) 23, 53
TaaI ACNGT 1 cut(s) 545
TaqI TCGA 5 cut(s) 14, 44, 169, 351, 526
TasI AATT 5 cut(s) 4, 34, 363, 522, 554
TatI WGTACW 2 cut(s) 313, 510
Tru1I TTAA 4 cut(s) 104, 540, 600, 606
Tru9I TTAA 4 cut(s) 104, 540, 600, 606
TseFI GTSAC 2 cut(s) 424, 434
Tsp45I GTSAC 2 cut(s) 424, 434
TspDTI ATGAA 5 cut(s) 17, 47, 187, 387, 472
TspGWI ACGGA 1 cut(s) 396
XapI RAATTY 1 cut(s) 363
XceI RCATGY 1 cut(s) 458
XcmI CCANNNNNNNNNTGG 1 cut(s) 124
XmaJI CCTAGG 2 cut(s) 23, 53
XspI CTAG 4 cut(s) 24, 54, 83, 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.