Rroxscaffold_1G00060800

protein phosphatase 2C 55

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0004533
Physical Location & Seq
Forward (+)
83039536 .. 83040528
993 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00060800.1

Sequence Viewer

Length: 993 bp
ATGACTCTCCAACTGCAGAGGGTACTCCACAATTTTAAACTTCAAGGCAATAATCTTGAGAGTTCGTCGACCATGGTCATGAACTATAATGGATCAATCAAACGCACACGAGCCGAGCCTGATCAAGAACAAGAGGCCGTGATCTTCAAGAAACCTAGGGTTGAAGGCAGCAAACCAGAGAGGTCCGTGATGCCGTACCAAGAACTGAGTCCAAACTATGAATCTGAAACAAGGCTGAAGATGGTATCTGGATCTTTCTATTTGCCCAAGGAAAACGAACTCCGACCCCAAGGTGAAGACGCTCACTTCATATGTGCAGAAGAAAACACCATTGGCGTGGCGGACGGTGTTGGTGGATGGGCTAACAGGGGCATCGACTCGGGTCTCTACGCTCGAGAACTCATGATGAACTCGGTCATGGCGGTTCACAATCAACACAAGACCTCATCAGGAGTCATTAATCCAAGAGAAGTGTTGGAGGAGGCTTTCTCGAACACCAAGAACATCCAAGGCTCGTCAACCGCTTGCATTGTCACGCTAAACAAAGATGTCTTGCACTATGCGAATGTTGGTGACAGTGGCTTCATGTTGTTTCGAAACAAGAAGTGCTTGTTCAAGTCTTCAACCCAACAGCACAGGTTTAACTGTCCTTTTCAGTTGGGGAACGCCCCAAGAAGTGATCGTCCGAGCTCGGCTACGGGGGTGCCGGTTAGGGTGATTCCGGGAGATGTCATAGTTCTTGGGACTGATGGATTGCTTGACAACATGTTTGGGAGGGAAATCGAGCAAGTTTTGGAGGACAAGACTGATGAAGGTGATGTGGATCCGGAGATGTTGGCTGGGATTATAGCGGAGTTGGCTTTGTATAATTCTTTTGATAGATTCAATCCTAGCCCTTTTTCAGTTTCGGCGAAGAAGGCTGGAGTCAAGCATGTAGGAGGTAAGGTTGATGATATCACTGTCATAGTAGCTGCCATTATGGAGGAGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoIIE

Protein Analysis

330

Amino Acids

36.42

Weight (kDa)

5.49

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoIIE PF07228 127 - 260 7.1e-06 Stage II sporulation protein E (SpoIIE)
PP2C PF00481 144 - 216 2.9e-06 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 703
AccI GTMKAC 1 cut(s) 68
AccIII TCCGGA 1 cut(s) 826
AciI CCGC 4 cut(s) 341, 422, 522, 851
AclWI GGATC 4 cut(s) 100, 259, 818, 831
AcuI CTGAAG 1 cut(s) 257
AfaI GTAC 2 cut(s) 24, 197
AflIII ACRYGT 1 cut(s) 765
AgsI TTSAA 6 cut(s) 44, 148, 164, 616, 624, 886
AjuI GAANNNNNNNTTGG 2 cut(s) 315, 347
AluBI AGCT 2 cut(s) 690, 971
AluI AGCT 2 cut(s) 690, 971
Alw21I GWGCWC 1 cut(s) 692
Alw26I GTCTC 1 cut(s) 389
AlwI GGATC 4 cut(s) 100, 259, 818, 831
Ama87I CYCGRG 2 cut(s) 379, 393
Aor13HI TCCGGA 1 cut(s) 826
AoxI GGCC 1 cut(s) 135
ApeKI GCWGC 2 cut(s) 168, 971
ArsI GACNNNNNNTTYG 2 cut(s) 752, 784
AseI ATTAAT 1 cut(s) 459
AspA2I CCTAGG 1 cut(s) 155
AspS9I GGNCC 1 cut(s) 183
AsuC2I CCSGG 1 cut(s) 723
AsuHPI GGTGA 4 cut(s) 305, 584, 727, 827
AsuII TTCGAA 1 cut(s) 595
AvaI CYCGRG 2 cut(s) 379, 393
AvaII GGWCC 1 cut(s) 183
AvrII CCTAGG 1 cut(s) 155
BamHI GGATCC 1 cut(s) 823
BanI GGYRCC 1 cut(s) 703
BanII GRGCYC 1 cut(s) 692
BauI CACGAG 1 cut(s) 108
BbsI GAAGAC 2 cut(s) 303, 612
Bbv12I GWGCWC 1 cut(s) 692
BbvI GCAGC 2 cut(s) 180, 958
BccI CCATC 3 cut(s) 235, 351, 743
BceAI ACGGC 2 cut(s) 122, 178
BclI TGATCA 1 cut(s) 121
BcnI CCSGG 1 cut(s) 723
BcoDI GTCTC 1 cut(s) 389
BfaI CTAG 3 cut(s) 156, 891, 991
BfmI CTRYAG 1 cut(s) 14
BisI GCNGC 2 cut(s) 169, 972
BlnI CCTAGG 1 cut(s) 155
BlsI GCNGC 2 cut(s) 170, 973
Bme1390I CCNGG 1 cut(s) 723
Bme18I GGWCC 1 cut(s) 183
BmeT110I CYCGRG 2 cut(s) 379, 393
BmgT120I GGNCC 1 cut(s) 183
BmiI GGNNCC 2 cut(s) 705, 825
BmrFI CCNGG 1 cut(s) 723
BmsI GCATC 2 cut(s) 180, 381
BoxI GACNNNNGTC 2 cut(s) 74, 381
BpiI GAAGAC 2 cut(s) 303, 612
BplI GAGNNNNNCTC 2 cut(s) 473, 505
BpmI CTGGAG 1 cut(s) 942
Bpu14I TTCGAA 1 cut(s) 595
BpuEI CTTGAG 1 cut(s) 77
BpuMI CCSGG 1 cut(s) 723
BsaBI GATNNNNATC 1 cut(s) 822
BsaI GGTCTC 1 cut(s) 389
BsaJI CCNNGG 5 cut(s) 72, 155, 267, 289, 508
BsaWI WCCGGW 1 cut(s) 826
BsaXI ACNNNNNCTCC 2 cut(s) 930, 960
Bse118I RCCGGY 1 cut(s) 706
Bse8I GATNNNNATC 1 cut(s) 822
BseAI TCCGGA 1 cut(s) 826
BseDI CCNNGG 5 cut(s) 72, 155, 267, 289, 508
BseGI GGATG 2 cut(s) 362, 504
BseJI GATNNNNATC 1 cut(s) 822
BseMII CTCAG 1 cut(s) 197
BseRI GAGGAG 1 cut(s) 494
BseXI GCAGC 2 cut(s) 180, 958
BseYI CCCAGC 1 cut(s) 839
BsgI GTGCAG 1 cut(s) 336
BshFI GGCC 1 cut(s) 137
BshNI GGYRCC 1 cut(s) 703
BsiHKAI GWGCWC 1 cut(s) 692
BsiHKCI CYCGRG 2 cut(s) 379, 393
BsiSI CCGG 3 cut(s) 707, 722, 827
BslFI GGGAC 1 cut(s) 757
BsmAI GTCTC 1 cut(s) 389
BsmFI GGGAC 1 cut(s) 757
BsnI GGCC 1 cut(s) 137
Bso31I GGTCTC 1 cut(s) 389
BsoBI CYCGRG 2 cut(s) 379, 393
Bsp119I TTCGAA 1 cut(s) 595
Bsp1286I GDGCHC 1 cut(s) 692
Bsp13I TCCGGA 1 cut(s) 826
Bsp143I GATC 6 cut(s) 92, 121, 141, 251, 679, 823
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 4 cut(s) 341, 422, 522, 851
BspANI GGCC 1 cut(s) 137
BspCNI CTCAG 1 cut(s) 198
BspEI TCCGGA 1 cut(s) 826
BspHI TCATGA 2 cut(s) 78, 402
BspLI GGNNCC 2 cut(s) 705, 825
BspMAI CTGCAG 1 cut(s) 18
BspPI GGATC 4 cut(s) 100, 259, 818, 831
BspT104I TTCGAA 1 cut(s) 595
BspT107I GGYRCC 1 cut(s) 703
BspTNI GGTCTC 1 cut(s) 389
BsrFI RCCGGY 1 cut(s) 706
BssAI RCCGGY 1 cut(s) 706
BssECI CCNNGG 5 cut(s) 72, 155, 267, 289, 508
BssMI GATC 6 cut(s) 92, 121, 141, 251, 679, 823
BssSI CACGAG 1 cut(s) 108
BssT1I CCWWGG 5 cut(s) 72, 155, 267, 289, 508
Bst2BI CACGAG 1 cut(s) 108
Bst4CI ACNGT 4 cut(s) 347, 578, 647, 961
BstBI TTCGAA 1 cut(s) 595
BstC8I GCNNGC 1 cut(s) 526
BstDEI CTNAG 1 cut(s) 206
BstDSI CCRYGG 1 cut(s) 72
BstF5I GGATG 2 cut(s) 362, 504
BstKTI GATC 6 cut(s) 95, 124, 144, 254, 682, 826
BstMAI GTCTC 1 cut(s) 389
BstMBI GATC 6 cut(s) 92, 121, 141, 251, 679, 823
BstMWI GCNNNNNNNGC 2 cut(s) 857, 917
BstNSI RCATGY 2 cut(s) 769, 935
BstPAI GACNNNNGTC 2 cut(s) 74, 381
BstSCI CCNGG 1 cut(s) 721
BstSFI CTRYAG 1 cut(s) 14
BstV1I GCAGC 2 cut(s) 180, 958
BstV2I GAAGAC 2 cut(s) 303, 612
BstX2I RGATCY 2 cut(s) 251, 823
BstXI CCANNNNNNTGG 1 cut(s) 337
BstYI RGATCY 2 cut(s) 251, 823
BsuRI GGCC 1 cut(s) 137
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 2 cut(s) 362, 504
BtsIMutI CAGTG 2 cut(s) 583, 957
Cac8I GCNNGC 1 cut(s) 526
CciI TCATGA 2 cut(s) 78, 402
Cfr10I RCCGGY 1 cut(s) 706
Cfr13I GGNCC 1 cut(s) 183
CseI GACGC 1 cut(s) 308
Csp6I GTAC 2 cut(s) 23, 196
CviAII CATG 7 cut(s) 73, 79, 403, 418, 586, 766, 932
CviQI GTAC 2 cut(s) 23, 196
DdeI CTNAG 1 cut(s) 206
DpnI GATC 6 cut(s) 94, 123, 143, 253, 681, 825
DpnII GATC 6 cut(s) 92, 121, 141, 251, 679, 823
DraI TTTAAA 1 cut(s) 37
EciI GGCGGA 1 cut(s) 356
Ecl136II GAGCTC 1 cut(s) 690
Eco130I CCWWGG 5 cut(s) 72, 155, 267, 289, 508
Eco24I GRGCYC 1 cut(s) 692
Eco31I GGTCTC 1 cut(s) 389
Eco32I GATATC 1 cut(s) 955
Eco47I GGWCC 1 cut(s) 183
Eco53kI GAGCTC 1 cut(s) 690
Eco57I CTGAAG 1 cut(s) 257
Eco88I CYCGRG 2 cut(s) 379, 393
EcoICRI GAGCTC 1 cut(s) 690
EcoRV GATATC 1 cut(s) 955
EcoT14I CCWWGG 5 cut(s) 72, 155, 267, 289, 508
EcoT38I GRGCYC 1 cut(s) 692
ErhI CCWWGG 5 cut(s) 72, 155, 267, 289, 508
FaeI CATG 7 cut(s) 76, 82, 406, 421, 589, 769, 935
FalI AAGNNNNNCTT 2 cut(s) 593, 625
FaqI GGGAC 1 cut(s) 757
FatI CATG 7 cut(s) 72, 78, 402, 417, 585, 765, 931
FauNDI CATATG 1 cut(s) 311
FbaI TGATCA 1 cut(s) 121
FblI GTMKAC 1 cut(s) 68
Fnu4HI GCNGC 2 cut(s) 169, 972
FokI GGATG 2 cut(s) 369, 491
FriOI GRGCYC 1 cut(s) 692
Fsp4HI GCNGC 2 cut(s) 169, 972
FspBI CTAG 3 cut(s) 156, 891, 991
GluI GCNGC 2 cut(s) 169, 972
GsaI CCCAGC 1 cut(s) 843
GsuI CTGGAG 1 cut(s) 942
HaeIII GGCC 1 cut(s) 137
HapII CCGG 3 cut(s) 707, 722, 827
HgaI GACGC 1 cut(s) 308
Hin1II CATG 7 cut(s) 76, 82, 406, 421, 589, 769, 935
HincII GTYRAC 2 cut(s) 69, 519
HindII GTYRAC 2 cut(s) 69, 519
HinfI GANTC 9 cut(s) 4, 208, 221, 377, 453, 718, 882, 924, 986
HpaII CCGG 3 cut(s) 707, 722, 827
HphI GGTGA 4 cut(s) 305, 584, 727, 827
Hpy166II GTNNAC 3 cut(s) 69, 427, 519
Hpy188I TCNGA 3 cut(s) 226, 284, 687
Hpy8I GTNNAC 3 cut(s) 69, 427, 519
Hpy99I CGWCG 1 cut(s) 70
HpyAV CCTTC 3 cut(s) 158, 806, 910
HpyCH4III ACNGT 4 cut(s) 347, 578, 647, 961
HpyCH4V TGCA 4 cut(s) 16, 317, 528, 556
HpyF10VI GCNNNNNNNGC 2 cut(s) 857, 917
HpyF3I CTNAG 1 cut(s) 206
Hsp92II CATG 7 cut(s) 76, 82, 406, 421, 589, 769, 935
Kpn2I TCCGGA 1 cut(s) 826
Ksp22I TGATCA 1 cut(s) 121
Kzo9I GATC 6 cut(s) 92, 121, 141, 251, 679, 823
Lsp1109I GCAGC 2 cut(s) 180, 958
LweI GCATC 2 cut(s) 180, 381
MaeI CTAG 3 cut(s) 156, 891, 991
MaeIII GTNAC 2 cut(s) 532, 572
MalI GATC 6 cut(s) 94, 123, 143, 253, 681, 825
MboI GATC 6 cut(s) 92, 121, 141, 251, 679, 823
MboII GAAGA 6 cut(s) 136, 250, 308, 332, 612, 925
MflI RGATCY 2 cut(s) 251, 823
MhlI GDGCHC 1 cut(s) 692
MluCI AATT 2 cut(s) 31, 868
MlyI GAGTC 4 cut(s) 217, 371, 462, 933
MmeI TCCRAC 3 cut(s) 34, 307, 456
MroI TCCGGA 1 cut(s) 826
MseI TTAA 3 cut(s) 36, 459, 642
MslI CAYNNNNRTG 2 cut(s) 77, 335
MspI CCGG 3 cut(s) 707, 722, 827
MspR9I CCNGG 1 cut(s) 723
MwoI GCNNNNNNNGC 2 cut(s) 857, 917
NciI CCSGG 1 cut(s) 723
NcoI CCATGG 1 cut(s) 72
NdeI CATATG 1 cut(s) 311
NdeII GATC 6 cut(s) 92, 121, 141, 251, 679, 823
NlaIII CATG 7 cut(s) 76, 82, 406, 421, 589, 769, 935
NlaIV GGNNCC 2 cut(s) 705, 825
NmeAIII GCCGAG 2 cut(s) 139, 671
NmuCI GTSAC 2 cut(s) 532, 572
NspI RCATGY 2 cut(s) 769, 935
NspV TTCGAA 1 cut(s) 595
PaeR7I CTCGAG 1 cut(s) 393
PagI TCATGA 2 cut(s) 78, 402
PciI ACATGT 1 cut(s) 765
PfeI GAWTC 3 cut(s) 221, 718, 882
PfoI TCCNGGA 1 cut(s) 721
PkrI GCNGC 2 cut(s) 170, 973
PleI GAGTC 4 cut(s) 216, 371, 461, 932
PpsI GAGTC 4 cut(s) 216, 371, 461, 932
PscI ACATGT 1 cut(s) 765
PshAI GACNNNNGTC 2 cut(s) 74, 381
PshBI ATTAAT 1 cut(s) 459
Psp124BI GAGCTC 1 cut(s) 692
PspFI CCCAGC 1 cut(s) 839
PspN4I GGNNCC 2 cut(s) 705, 825
PspPI GGNCC 1 cut(s) 183
PstI CTGCAG 1 cut(s) 18
PsuI RGATCY 2 cut(s) 251, 823
RsaI GTAC 2 cut(s) 24, 197
RsaNI GTAC 2 cut(s) 23, 196
RseI CAYNNNNRTG 2 cut(s) 77, 335
SacI GAGCTC 1 cut(s) 692
SalI GTCGAC 1 cut(s) 67
SaqAI TTAA 3 cut(s) 36, 459, 642
SatI GCNGC 2 cut(s) 169, 972
Sau3AI GATC 6 cut(s) 92, 121, 141, 251, 679, 823
Sau96I GGNCC 1 cut(s) 183
SchI GAGTC 4 cut(s) 217, 371, 462, 933
ScrFI CCNGG 1 cut(s) 723
SduI GDGCHC 1 cut(s) 692
SfaNI GCATC 2 cut(s) 180, 381
SfcI CTRYAG 1 cut(s) 14
Sfr274I CTCGAG 1 cut(s) 393
SfuI TTCGAA 1 cut(s) 595
SinI GGWCC 1 cut(s) 183
SlaI CTCGAG 1 cut(s) 393
SmiMI CAYNNNNRTG 2 cut(s) 77, 335
SmlI CTYRAG 2 cut(s) 56, 393
SmoI CTYRAG 2 cut(s) 56, 393
Sse9I AATT 2 cut(s) 31, 868
SsiI CCGC 4 cut(s) 341, 422, 522, 851
SspMI CTAG 3 cut(s) 156, 891, 991
SstI GAGCTC 1 cut(s) 692
StyD4I CCNGG 1 cut(s) 721
StyI CCWWGG 5 cut(s) 72, 155, 267, 289, 508
TaaI ACNGT 4 cut(s) 347, 578, 647, 961
TaqI TCGA 6 cut(s) 68, 375, 394, 491, 595, 783
TaqII GACCGA 1 cut(s) 403
TasI AATT 2 cut(s) 31, 868
TfiI GAWTC 3 cut(s) 221, 718, 882
Tru1I TTAA 3 cut(s) 36, 459, 642
Tru9I TTAA 3 cut(s) 36, 459, 642
TscAI CASTG 2 cut(s) 583, 964
TseFI GTSAC 2 cut(s) 532, 572
TseI GCWGC 2 cut(s) 168, 971
Tsp45I GTSAC 2 cut(s) 532, 572
TspDTI ATGAA 6 cut(s) 95, 234, 298, 422, 574, 825
TspGWI ACGGA 1 cut(s) 175
TspRI CASTG 2 cut(s) 583, 964
VpaK11BI GGWCC 1 cut(s) 183
VspI ATTAAT 1 cut(s) 459
XceI RCATGY 2 cut(s) 769, 935
XhoI CTCGAG 1 cut(s) 393
XmaJI CCTAGG 1 cut(s) 155
XmiI GTMKAC 1 cut(s) 68
XspI CTAG 3 cut(s) 156, 891, 991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.