RLG00000012617

Protein phosphatase 2C 55

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004533
Physical Location & Seq
Forward (+)
20553637 .. 20554597
961 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012617

Sequence Viewer

Length: 780 bp
ATGGTCTGTGGGTCTTTTTATTTGCCCAAAGAAAGTGAAAATCGATCGCTACTAGGCGAAGACGCTCACTTCATTTGTGCAAAAGAGAACACCATTGGTGTTGCGGATGGTGTTGGCGGGTGGGTTAAGTATGGCGTGGATGCTGGAGAGTATGCAAGGAAGCTCATGGACAACTCAGTCCTGGCTATACATAACCAACACAGAAAATCTAGAGATGCTGATGTCGATCCAATACAAATCTTGGATGAAGCCTACGGAAACACCAAGAATATTGCTGGGACATCAACAGCTTGCATCGTCACCCTTAGTAAAGAAGGTAGAACCTTGCATGCTGTGAATGTTGGCGATAGTGGTTTCATGCTGTTTAGAGACAAGAAGTGTGTGTACATTTCGCCAATTCGACAGAGTTGCTTTAACTGTCCTCTTCAATTGGGGACAGGATCGGGAGATACTCCCCAATCGGCTACCGAGATTAAAGTTAGGGTACTTCGGCCGGGAGATGTCATAGTCCTTGGGACAGATGGGTTACTGGACAATGTGTCGCCTGGAGAAATAGAGAAAGTTCTGGAGGAGAATACCATAGAGGGTTTTGTGGAACCGGAGATGTTGGCATGGATCATAGCAGAGAGGCTGGCTTTACGCAACTCCGAGGATGCAGGAAGGTTGACTCCTTTTTCAATTGCGGCTCGTAAGGCCGGAGTCGAGCACATTGGAGAGTCAGATCATGTATGTTGTGGTAGAGCCATTCATCATATGTTCCCCTGGCCTTCGAAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoIIE

Protein Analysis

260

Amino Acids

28.2

Weight (kDa)

5.51

Isoelectric Point (pI)

41.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoIIE PF07228 67 - 185 1.1e-06 Stage II sporulation protein E (SpoIIE)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 176
AciI CCGC 3 cut(s) 104, 117, 683
AclWI GGATC 3 cut(s) 221, 448, 623
AcoI YGGCCR 1 cut(s) 491
AfaI GTAC 2 cut(s) 386, 486
AgsI TTSAA 2 cut(s) 428, 678
AhdI GACNNNNNGTC 1 cut(s) 538
AjnI CCWGG 3 cut(s) 180, 544, 761
AluBI AGCT 2 cut(s) 163, 290
AluI AGCT 2 cut(s) 163, 290
Alw21I GWGCWC 1 cut(s) 708
Alw26I GTCTC 1 cut(s) 363
AlwI GGATC 3 cut(s) 221, 448, 623
AoxI GGCC 3 cut(s) 491, 693, 764
AsuC2I CCSGG 1 cut(s) 495
AsuHPI GGTGA 1 cut(s) 292
AsuII TTCGAA 1 cut(s) 770
BarI GAAGNNNNNNTAC 2 cut(s) 368, 400
BbsI GAAGAC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 708
BccI CCATC 2 cut(s) 101, 515
BcgI CGANNNNNNTGC 2 cut(s) 390, 424
BciT130I CCWGG 3 cut(s) 182, 546, 763
BcnI CCSGG 1 cut(s) 495
BcoDI GTCTC 1 cut(s) 363
BfaI CTAG 2 cut(s) 53, 210
BisI GCNGC 1 cut(s) 684
BlsI GCNGC 1 cut(s) 685
Bme1390I CCNGG 4 cut(s) 182, 495, 546, 763
BmeRI GACNNNNNGTC 1 cut(s) 538
BmiI GGNNCC 1 cut(s) 597
BmrFI CCNGG 4 cut(s) 182, 495, 546, 763
BmsI GCATC 4 cut(s) 130, 205, 303, 643
BpiI GAAGAC 1 cut(s) 66
BpmI CTGGAG 3 cut(s) 165, 567, 587
Bpu14I TTCGAA 1 cut(s) 770
BpuMI CCSGG 1 cut(s) 495
Bsa29I ATCGAT 1 cut(s) 43
BsaBI GATNNNNATC 1 cut(s) 225
BsaJI CCNNGG 3 cut(s) 511, 648, 761
BsaWI WCCGGW 1 cut(s) 598
Bse1I ACTGG 1 cut(s) 534
Bse8I GATNNNNATC 1 cut(s) 225
BseBI CCWGG 3 cut(s) 182, 546, 763
BseCI ATCGAT 1 cut(s) 43
BseDI CCNNGG 3 cut(s) 511, 648, 761
BseGI GGATG 4 cut(s) 112, 145, 250, 658
BseJI GATNNNNATC 1 cut(s) 225
BseMII CTCAG 1 cut(s) 189
BseNI ACTGG 1 cut(s) 534
BseRI GAGGAG 1 cut(s) 584
BseX3I CGGCCG 1 cut(s) 491
BseYI CCCAGC 1 cut(s) 275
Bsh1285I CGRYCG 2 cut(s) 47, 494
BshFI GGCC 3 cut(s) 493, 695, 766
BshVI ATCGAT 1 cut(s) 43
BsiEI CGRYCG 2 cut(s) 47, 494
BsiHKAI GWGCWC 1 cut(s) 708
BsiSI CCGG 3 cut(s) 494, 599, 696
BslFI GGGAC 3 cut(s) 292, 448, 529
BsmAI GTCTC 1 cut(s) 363
BsmFI GGGAC 3 cut(s) 292, 448, 529
BsnI GGCC 3 cut(s) 493, 695, 766
Bsp119I TTCGAA 1 cut(s) 770
Bsp1286I GDGCHC 1 cut(s) 708
Bsp1407I TGTACA 1 cut(s) 384
Bsp143I GATC 5 cut(s) 44, 226, 440, 615, 721
BspACI CCGC 3 cut(s) 104, 117, 683
BspANI GGCC 3 cut(s) 493, 695, 766
BspCNI CTCAG 1 cut(s) 188
BspDI ATCGAT 1 cut(s) 43
BspLI GGNNCC 1 cut(s) 597
BspPI GGATC 3 cut(s) 221, 448, 623
BspT104I TTCGAA 1 cut(s) 770
BsrGI TGTACA 1 cut(s) 384
BsrI ACTGG 1 cut(s) 534
BssECI CCNNGG 3 cut(s) 511, 648, 761
BssMI GATC 5 cut(s) 44, 226, 440, 615, 721
BssT1I CCWWGG 1 cut(s) 511
Bst2UI CCWGG 3 cut(s) 182, 546, 763
Bst4CI ACNGT 1 cut(s) 419
Bst6I CTCTTC 1 cut(s) 429
BstAUI TGTACA 1 cut(s) 384
BstBI TTCGAA 1 cut(s) 770
BstC8I GCNNGC 3 cut(s) 292, 330, 633
BstDEI CTNAG 2 cut(s) 175, 305
BstF5I GGATG 4 cut(s) 112, 145, 250, 658
BstKTI GATC 5 cut(s) 47, 229, 443, 618, 724
BstMAI GTCTC 1 cut(s) 363
BstMBI GATC 5 cut(s) 44, 226, 440, 615, 721
BstMCI CGRYCG 2 cut(s) 47, 494
BstMWI GCNNNNNNNGC 1 cut(s) 692
BstNI CCWGG 3 cut(s) 182, 546, 763
BstNSI RCATGY 1 cut(s) 332
BstSCI CCNGG 4 cut(s) 180, 493, 544, 761
BstV2I GAAGAC 1 cut(s) 66
BstZI CGGCCG 1 cut(s) 491
Bsu15I ATCGAT 1 cut(s) 43
BsuRI GGCC 3 cut(s) 493, 695, 766
BsuTUI ATCGAT 1 cut(s) 43
BtsCI GGATG 4 cut(s) 112, 145, 250, 658
Cac8I GCNNGC 3 cut(s) 292, 330, 633
ClaI ATCGAT 1 cut(s) 43
CseI GACGC 1 cut(s) 71
Csp6I GTAC 2 cut(s) 385, 485
CviAII CATG 5 cut(s) 166, 329, 358, 612, 725
CviQI GTAC 2 cut(s) 385, 485
DdeI CTNAG 2 cut(s) 175, 305
DpnI GATC 5 cut(s) 46, 228, 442, 617, 723
DpnII GATC 5 cut(s) 44, 226, 440, 615, 721
DrdI GACNNNNNNGTC 1 cut(s) 176
DriI GACNNNNNGTC 1 cut(s) 538
DseDI GACNNNNNNGTC 1 cut(s) 176
EaeI YGGCCR 1 cut(s) 491
EagI CGGCCG 1 cut(s) 491
Eam1104I CTCTTC 1 cut(s) 429
Eam1105I GACNNNNNGTC 1 cut(s) 538
EarI CTCTTC 1 cut(s) 429
EclXI CGGCCG 1 cut(s) 491
Eco130I CCWWGG 1 cut(s) 511
Eco52I CGGCCG 1 cut(s) 491
EcoRII CCWGG 3 cut(s) 180, 544, 761
EcoT14I CCWWGG 1 cut(s) 511
ErhI CCWWGG 1 cut(s) 511
FaeI CATG 5 cut(s) 169, 332, 361, 615, 728
FaqI GGGAC 3 cut(s) 292, 448, 529
FatI CATG 5 cut(s) 165, 328, 357, 611, 724
FauI CCCGC 1 cut(s) 110
FauNDI CATATG 1 cut(s) 753
Fnu4HI GCNGC 1 cut(s) 684
FokI GGATG 4 cut(s) 119, 152, 257, 665
Fsp4HI GCNGC 1 cut(s) 684
FspBI CTAG 2 cut(s) 53, 210
GluI GCNGC 1 cut(s) 684
GsaI CCCAGC 1 cut(s) 279
GsuI CTGGAG 3 cut(s) 165, 567, 587
HaeIII GGCC 3 cut(s) 493, 695, 766
HapII CCGG 3 cut(s) 494, 599, 696
HgaI GACGC 1 cut(s) 71
Hin1II CATG 5 cut(s) 169, 332, 361, 615, 728
HincII GTYRAC 1 cut(s) 666
HindII GTYRAC 1 cut(s) 666
HinfI GANTC 3 cut(s) 667, 699, 716
HpaII CCGG 3 cut(s) 494, 599, 696
HphI GGTGA 1 cut(s) 292
Hpy166II GTNNAC 2 cut(s) 385, 666
Hpy188I TCNGA 2 cut(s) 649, 721
Hpy188III TCNNGA 3 cut(s) 210, 444, 566
Hpy8I GTNNAC 2 cut(s) 385, 666
HpyAV CCTTC 3 cut(s) 308, 654, 777
HpyCH4III ACNGT 1 cut(s) 419
HpyCH4V TGCA 5 cut(s) 80, 155, 294, 328, 656
HpyF10VI GCNNNNNNNGC 1 cut(s) 692
HpyF3I CTNAG 2 cut(s) 175, 305
Hsp92II CATG 5 cut(s) 169, 332, 361, 615, 728
Kzo9I GATC 5 cut(s) 44, 226, 440, 615, 721
LweI GCATC 4 cut(s) 130, 205, 303, 643
MaeI CTAG 2 cut(s) 53, 210
MaeIII GTNAC 2 cut(s) 298, 525
MalI GATC 5 cut(s) 46, 228, 442, 617, 723
MboI GATC 5 cut(s) 44, 226, 440, 615, 721
MboII GAAGA 2 cut(s) 71, 416
MfeI CAATTG 2 cut(s) 428, 678
MhlI GDGCHC 1 cut(s) 708
MluCI AATT 4 cut(s) 396, 428, 678, 775
MlyI GAGTC 3 cut(s) 661, 708, 725
MnlI CCTC 5 cut(s) 432, 562, 577, 621, 643
MseI TTAA 3 cut(s) 126, 414, 474
MspI CCGG 3 cut(s) 494, 599, 696
MspR9I CCNGG 4 cut(s) 182, 495, 546, 763
MunI CAATTG 2 cut(s) 428, 678
MvaI CCWGG 3 cut(s) 182, 546, 763
MwoI GCNNNNNNNGC 1 cut(s) 692
NciI CCSGG 1 cut(s) 495
NdeI CATATG 1 cut(s) 753
NdeII GATC 5 cut(s) 44, 226, 440, 615, 721
NlaIII CATG 5 cut(s) 169, 332, 361, 615, 728
NlaIV GGNNCC 1 cut(s) 597
NmuCI GTSAC 1 cut(s) 298
NspI RCATGY 1 cut(s) 332
NspV TTCGAA 1 cut(s) 770
PaeI GCATGC 1 cut(s) 332
PkrI GCNGC 1 cut(s) 685
Ple19I CGATCG 1 cut(s) 47
PleI GAGTC 3 cut(s) 661, 707, 724
PpsI GAGTC 3 cut(s) 661, 707, 724
Psp6I CCWGG 3 cut(s) 180, 544, 761
PspFI CCCAGC 1 cut(s) 275
PspGI CCWGG 3 cut(s) 180, 544, 761
PspN4I GGNNCC 1 cut(s) 597
PvuI CGATCG 1 cut(s) 47
RsaI GTAC 2 cut(s) 386, 486
RsaNI GTAC 2 cut(s) 385, 485
SaqAI TTAA 3 cut(s) 126, 414, 474
SatI GCNGC 1 cut(s) 684
Sau3AI GATC 5 cut(s) 44, 226, 440, 615, 721
SchI GAGTC 3 cut(s) 661, 708, 725
ScrFI CCNGG 4 cut(s) 182, 495, 546, 763
SduI GDGCHC 1 cut(s) 708
SetI ASST 5 cut(s) 165, 292, 319, 326, 665
SfaNI GCATC 4 cut(s) 130, 205, 303, 643
SfuI TTCGAA 1 cut(s) 770
SphI GCATGC 1 cut(s) 332
Sse9I AATT 4 cut(s) 396, 428, 678, 775
SsiI CCGC 3 cut(s) 104, 117, 683
SspI AATATT 1 cut(s) 271
SspMI CTAG 2 cut(s) 53, 210
StyD4I CCNGG 4 cut(s) 180, 493, 544, 761
StyI CCWWGG 1 cut(s) 511
TaaI ACNGT 1 cut(s) 419
TaqI TCGA 5 cut(s) 43, 225, 400, 702, 770
TasI AATT 4 cut(s) 396, 428, 678, 775
TatI WGTACW 1 cut(s) 384
TauI GCSGC 1 cut(s) 686
Tru1I TTAA 3 cut(s) 126, 414, 474
Tru9I TTAA 3 cut(s) 126, 414, 474
TseFI GTSAC 1 cut(s) 298
Tsp45I GTSAC 1 cut(s) 298
TspDTI ATGAA 4 cut(s) 61, 261, 346, 737
TspGWI ACGGA 1 cut(s) 270
XbaI TCTAGA 1 cut(s) 209
XceI RCATGY 1 cut(s) 332
XspI CTAG 2 cut(s) 53, 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.