FvH4_3g37510

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001201
Physical Location & Seq
Forward (+)
32192040 .. 32199582
7543 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g37510.t2

Sequence Viewer

Length: 900 bp
ATGTACCAGAAAATTGCTAATAAGCTTCATCAGCTAGAGTCTCTTGACTCTCTTATGTCAACCCCAAACCCAAACAGTGCGAAGCTGAAAATGTTTAAGACCATGTTGGAGCGCCTCATGGCAGTCCTACAGCTTTCCAAAAGTAACATTTCACCTGGTTTGAAGGAGAAATTGAATGTATATGAGAAGCAGATAATAAATTTCATTAATACAAACAGACCCAGGAAGCCTGTTTCTTCTCTAAATCTTCCTCCATCTGAGATTCACAATGCACACATGCTGCAACAAGCCAAGGTGGAGCCACAAGATGGACAGCAGTGTCAACCTCATCCATCACAGCAAAAGTATTTTTCAGAGATCCAACCACAGATTGGACATATGCAACAACACCAGCTGACCTTGCAACAACACCAGTTGAGCTTACAACAACAGTCAAACCCACTACAGCGAGATATGCAGCATAGGCTTCAAGCATCAGGTCAGGTATCTGGAGCCATACTTCAACCACCAAATACAATGGAGCTGCAAAACCAGTTATATCAATCACATAGATCCCTTTCGGAGACCTCATCAAGCTCTCTAGATTACCCTGCCCAGACTGGACATACAGATTGGCAAGAAGAGGCCAATCAAAAGATTAAGGTCATGAAGCATTTATACTTTGCCGAACTAAGTGAGACGTACTGGAAGATAGCAACTAAACTTCAGCTGCAGGATTCTCATCTTCAACAGCAAACATCTGACCAGTATGACAAGCTGAGCATGTTGAAGAACAAGCTGGAGGACCTCATAATAGTCTTGCACTATTCCAAAACTGACATTTCACTCGTTTTGAAGGAGCAGTTGGTTTTCTACGAGAAGCAGATAGTAGATTTCATTGATACAAATAGAATCAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

300

Amino Acids

34.72

Weight (kDa)

8.73

Isoelectric Point (pI)

55.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 318
AccB7I CCANNNNNTGG 2 cut(s) 308, 371
AclWI GGATC 2 cut(s) 352, 546
AcsI RAATTY 1 cut(s) 199
AcuI CTGAAG 1 cut(s) 689
AfaI GTAC 2 cut(s) 5, 683
AfiI CCNNNNNNNGG 2 cut(s) 308, 371
AgsI TTSAA 7 cut(s) 163, 175, 470, 503, 728, 769, 835
AjnI CCWGG 2 cut(s) 154, 221
AjuI GAANNNNNNNTTGG 2 cut(s) 827, 859
Alw26I GTCTC 3 cut(s) 45, 557, 671
AlwI GGATC 2 cut(s) 352, 546
AoxI GGCC 1 cut(s) 624
ApeKI GCWGC 4 cut(s) 280, 457, 523, 709
ApoI RAATTY 1 cut(s) 199
AseI ATTAAT 1 cut(s) 207
AspLEI GCGC 1 cut(s) 114
AspS9I GGNCC 1 cut(s) 784
AsuHPI GGTGA 1 cut(s) 144
AvaII GGWCC 1 cut(s) 784
BarI GAAGNNNNNNTAC 2 cut(s) 641, 673
BbvI GCAGC 4 cut(s) 267, 469, 510, 696
BccI CCATC 3 cut(s) 262, 302, 340
BciT130I CCWGG 2 cut(s) 156, 223
BcoDI GTCTC 3 cut(s) 45, 557, 671
BfaI CTAG 2 cut(s) 35, 581
BfmI CTRYAG 3 cut(s) 128, 443, 710
BfoI RGCGCY 1 cut(s) 115
BisI GCNGC 4 cut(s) 281, 458, 524, 710
BlpI GCTNAGC 1 cut(s) 758
BlsI GCNGC 4 cut(s) 282, 459, 525, 711
Bme1390I CCNGG 2 cut(s) 156, 223
Bme18I GGWCC 1 cut(s) 784
BmgT120I GGNCC 1 cut(s) 784
BmiI GGNNCC 2 cut(s) 300, 493
BmrFI CCNGG 2 cut(s) 156, 223
BmsI GCATC 1 cut(s) 482
BpmI CTGGAG 2 cut(s) 510, 800
Bpu1102I GCTNAGC 1 cut(s) 758
BsaBI GATNNNNATC 1 cut(s) 720
BsaI GGTCTC 1 cut(s) 557
BsaJI CCNNGG 2 cut(s) 221, 291
Bsc4I CCNNNNNNNGG 2 cut(s) 308, 371
Bse1I ACTGG 5 cut(s) 412, 532, 604, 689, 745
Bse8I GATNNNNATC 1 cut(s) 720
BseBI CCWGG 2 cut(s) 156, 223
BseDI CCNNGG 2 cut(s) 221, 291
BseGI GGATG 1 cut(s) 328
BseJI GATNNNNATC 1 cut(s) 720
BseLI CCNNNNNNNGG 2 cut(s) 308, 371
BseMII CTCAG 2 cut(s) 249, 749
BseNI ACTGG 5 cut(s) 412, 532, 604, 689, 745
BseXI GCAGC 4 cut(s) 267, 469, 510, 696
BshFI GGCC 1 cut(s) 626
BslI CCNNNNNNNGG 2 cut(s) 308, 371
BsmAI GTCTC 3 cut(s) 45, 557, 671
BsmBI CGTCTC 1 cut(s) 671
BsnI GGCC 1 cut(s) 626
Bso31I GGTCTC 1 cut(s) 557
Bsp143I GATC 2 cut(s) 357, 551
Bsp1720I GCTNAGC 1 cut(s) 758
BspANI GGCC 1 cut(s) 626
BspCNI CTCAG 2 cut(s) 250, 750
BspHI TCATGA 1 cut(s) 645
BspLI GGNNCC 2 cut(s) 300, 493
BspMAI CTGCAG 1 cut(s) 714
BspPI GGATC 2 cut(s) 352, 546
BspTNI GGTCTC 1 cut(s) 557
BsrI ACTGG 5 cut(s) 412, 532, 604, 689, 745
BssECI CCNNGG 2 cut(s) 221, 291
BssMI GATC 2 cut(s) 357, 551
BssT1I CCWWGG 1 cut(s) 291
Bst2UI CCWGG 2 cut(s) 156, 223
Bst4CI ACNGT 2 cut(s) 77, 432
Bst6I CTCTTC 1 cut(s) 615
BstDEI CTNAG 3 cut(s) 258, 671, 758
BstF5I GGATG 1 cut(s) 328
BstH2I RGCGCY 1 cut(s) 115
BstHHI GCGC 1 cut(s) 114
BstKTI GATC 2 cut(s) 360, 554
BstMAI GTCTC 3 cut(s) 45, 557, 671
BstMBI GATC 2 cut(s) 357, 551
BstMWI GCNNNNNNNGC 4 cut(s) 31, 400, 454, 463
BstNI CCWGG 2 cut(s) 156, 223
BstNSI RCATGY 2 cut(s) 280, 766
BstSCI CCNGG 2 cut(s) 154, 221
BstSFI CTRYAG 3 cut(s) 128, 443, 710
BstV1I GCAGC 4 cut(s) 267, 469, 510, 696
BstX2I RGATCY 2 cut(s) 357, 551
BstYI RGATCY 2 cut(s) 357, 551
BsuRI GGCC 1 cut(s) 626
BtsCI GGATG 1 cut(s) 328
BtsI GCAGTG 1 cut(s) 323
BtsIMutI CAGTG 2 cut(s) 82, 323
CciI TCATGA 1 cut(s) 645
CfoI GCGC 1 cut(s) 114
Cfr13I GGNCC 1 cut(s) 784
CsiI ACCWGGT 1 cut(s) 154
Csp6I GTAC 2 cut(s) 4, 682
CviAII CATG 5 cut(s) 103, 118, 277, 646, 763
CviQI GTAC 2 cut(s) 4, 682
DdeI CTNAG 3 cut(s) 258, 671, 758
DpnI GATC 2 cut(s) 359, 553
DpnII GATC 2 cut(s) 357, 551
DrdI GACNNNNNNGTC 1 cut(s) 318
DseDI GACNNNNNNGTC 1 cut(s) 318
Eam1104I CTCTTC 1 cut(s) 615
EarI CTCTTC 1 cut(s) 615
Eco130I CCWWGG 1 cut(s) 291
Eco31I GGTCTC 1 cut(s) 557
Eco47I GGWCC 1 cut(s) 784
Eco57I CTGAAG 1 cut(s) 689
EcoO109I RGGNCCY 1 cut(s) 784
EcoRII CCWGG 2 cut(s) 154, 221
EcoT14I CCWWGG 1 cut(s) 291
ErhI CCWWGG 1 cut(s) 291
Esp3I CGTCTC 1 cut(s) 671
FaeI CATG 5 cut(s) 106, 121, 280, 649, 766
FatI CATG 5 cut(s) 102, 117, 276, 645, 762
FauNDI CATATG 1 cut(s) 378
Fnu4HI GCNGC 4 cut(s) 281, 458, 524, 710
FokI GGATG 1 cut(s) 315
Fsp4HI GCNGC 4 cut(s) 281, 458, 524, 710
FspBI CTAG 2 cut(s) 35, 581
GlaI GCGC 1 cut(s) 113
GluI GCNGC 4 cut(s) 281, 458, 524, 710
GsuI CTGGAG 2 cut(s) 510, 800
HaeII RGCGCY 1 cut(s) 115
HaeIII GGCC 1 cut(s) 626
HhaI GCGC 1 cut(s) 114
Hin1II CATG 5 cut(s) 106, 121, 280, 649, 766
Hin6I GCGC 1 cut(s) 112
HinP1I GCGC 1 cut(s) 112
HincII GTYRAC 2 cut(s) 60, 323
HindII GTYRAC 2 cut(s) 60, 323
HindIII AAGCTT 1 cut(s) 23
HinfI GANTC 5 cut(s) 38, 47, 262, 716, 891
HphI GGTGA 1 cut(s) 144
Hpy166II GTNNAC 2 cut(s) 60, 323
Hpy188I TCNGA 4 cut(s) 259, 355, 562, 742
Hpy188III TCNNGA 4 cut(s) 44, 489, 581, 646
Hpy8I GTNNAC 2 cut(s) 60, 323
HpyAV CCTTC 2 cut(s) 157, 829
HpyCH4III ACNGT 2 cut(s) 77, 432
HpyCH4IV ACGT 1 cut(s) 680
HpyCH4V TGCA 8 cut(s) 272, 283, 382, 403, 457, 526, 712, 802
HpyF10VI GCNNNNNNNGC 4 cut(s) 31, 400, 454, 463
HpyF3I CTNAG 3 cut(s) 258, 671, 758
HpySE526I ACGT 1 cut(s) 680
Hsp92II CATG 5 cut(s) 106, 121, 280, 649, 766
HspAI GCGC 1 cut(s) 112
Kzo9I GATC 2 cut(s) 357, 551
LmnI GCTCC 5 cut(s) 109, 298, 491, 520, 838
Lsp1109I GCAGC 4 cut(s) 267, 469, 510, 696
LweI GCATC 1 cut(s) 482
MabI ACCWGGT 1 cut(s) 154
MaeI CTAG 2 cut(s) 35, 581
MaeII ACGT 1 cut(s) 680
MaeIII GTNAC 1 cut(s) 143
MalI GATC 2 cut(s) 359, 553
MboI GATC 2 cut(s) 357, 551
MboII GAAGA 6 cut(s) 228, 239, 632, 700, 716, 781
MflI RGATCY 2 cut(s) 357, 551
MluCI AATT 3 cut(s) 12, 170, 199
MlyI GAGTC 2 cut(s) 41, 47
MmeI TCCRAC 2 cut(s) 87, 385
MnlI CCTC 7 cut(s) 125, 261, 336, 577, 616, 775, 797
MseI TTAA 3 cut(s) 96, 207, 639
MspA1I CMGCKG 2 cut(s) 394, 709
MspR9I CCNGG 2 cut(s) 156, 223
MvaI CCWGG 2 cut(s) 156, 223
MwoI GCNNNNNNNGC 4 cut(s) 31, 400, 454, 463
NdeI CATATG 1 cut(s) 378
NdeII GATC 2 cut(s) 357, 551
NlaIII CATG 5 cut(s) 106, 121, 280, 649, 766
NlaIV GGNNCC 2 cut(s) 300, 493
NspI RCATGY 2 cut(s) 280, 766
PagI TCATGA 1 cut(s) 645
PfeI GAWTC 3 cut(s) 262, 716, 891
PflMI CCANNNNNTGG 2 cut(s) 308, 371
PkrI GCNGC 4 cut(s) 282, 459, 525, 711
PleI GAGTC 2 cut(s) 41, 46
PpsI GAGTC 2 cut(s) 41, 46
PpuMI RGGWCCY 1 cut(s) 784
PshBI ATTAAT 1 cut(s) 207
Psp5II RGGWCCY 1 cut(s) 784
Psp6I CCWGG 2 cut(s) 154, 221
PspGI CCWGG 2 cut(s) 154, 221
PspN4I GGNNCC 2 cut(s) 300, 493
PspPI GGNCC 1 cut(s) 784
PspPPI RGGWCCY 1 cut(s) 784
PstI CTGCAG 1 cut(s) 714
PsuI RGATCY 2 cut(s) 357, 551
PvuII CAGCTG 2 cut(s) 394, 709
RsaI GTAC 2 cut(s) 5, 683
RsaNI GTAC 2 cut(s) 4, 682
SaqAI TTAA 3 cut(s) 96, 207, 639
SatI GCNGC 4 cut(s) 281, 458, 524, 710
Sau3AI GATC 2 cut(s) 357, 551
Sau96I GGNCC 1 cut(s) 784
SchI GAGTC 2 cut(s) 41, 47
ScrFI CCNGG 2 cut(s) 156, 223
SexAI ACCWGGT 1 cut(s) 154
SfaNI GCATC 1 cut(s) 482
SfcI CTRYAG 3 cut(s) 128, 443, 710
SinI GGWCC 1 cut(s) 784
Sse9I AATT 3 cut(s) 12, 170, 199
SspMI CTAG 2 cut(s) 35, 581
StyD4I CCNGG 2 cut(s) 154, 221
StyI CCWWGG 1 cut(s) 291
TaaI ACNGT 2 cut(s) 77, 432
TaiI ACGT 1 cut(s) 683
TasI AATT 3 cut(s) 12, 170, 199
TfiI GAWTC 3 cut(s) 262, 716, 891
Tru1I TTAA 3 cut(s) 96, 207, 639
Tru9I TTAA 3 cut(s) 96, 207, 639
TscAI CASTG 2 cut(s) 82, 323
TseI GCWGC 4 cut(s) 280, 457, 523, 709
TspDTI ATGAA 4 cut(s) 17, 193, 662, 865
TspRI CASTG 2 cut(s) 82, 323
Van91I CCANNNNNTGG 2 cut(s) 308, 371
VpaK11BI GGWCC 1 cut(s) 784
VspI ATTAAT 1 cut(s) 207
XapI RAATTY 1 cut(s) 199
XbaI TCTAGA 1 cut(s) 580
XceI RCATGY 2 cut(s) 280, 766
XcmI CCANNNNNNNNNTGG 1 cut(s) 368
XspI CTAG 2 cut(s) 35, 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.