RchiOBHm_Chr5g0075821

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001201
Physical Location & Seq
Forward (+)
81560650 .. 81564633
3984 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35052

Sequence Viewer

Length: 1056 bp
ATGAAGGAGACTTACTTGCCTGAATTAAGTGAAATGTTTCAGAAAATTGATAATAAACTCCAGGAGTACGAGTCTGTTCCGCCACAGTCAAATCCAGATAAGCTTGAGAAGCTGAAAGTGTTTAGGACCATGTTGGAGCACCTCATAACAGTCTTACAGGTTTCCAAAAGTAATATTTCACCTGGTTTGAAGGAGAAGTTGGGTTTATACGAGAAGCAGATCATAAATTTCATTAATACAAATAGACCCAGGAAGACTGTTTCTTCTCTACATCTTCCCCCACCTGATATGCACAATGCACACCTGCTGCAACAACCTAAGGTGGAGCAACAAGATCAGAGTGCATCTAACTTAATACCTACTCAAGGACAGCAGTCTCAACCTCATCCATCACAGCAACAATATCTTTCAAATATTCAATCTCAGCTTATACATATACAACAACGACAGTTGAGCATGCAACAACAGTTAATCCCACTACTGCAAGATATGCAGCAAAGGCACAAAGCATCGGGTCAGGTATCGGCAGCCATAATTCAACCACGAAATACAATGGAGCTGCAAAACCAGTTATATCAATCACATACAGCCATTCCGGAGACATCATCAACATCTCTAGATTCACCTGCCCTGACTGGACATACAGACAAGAGGCCTATCAAAAGGTTCATGAAGTACAACGATGGGAGTCCTCCTCAAGGTGGAGAGCCTGCAATGGATGTAGGAGATTGGAGGAGCCAATTGCAGGCGGATTCGCGGCAAAGAATTGTCAATAAGCTTATGGATCTATTGAAGAGGCATCTTCCTTTCTCCAGTCAAGAGGGATTGGACGAACTGAAGAAAATTTCTGTTAGATTTGAGGAAAAGATTTATGGTGCTGCCACAAGTCAGTCAGATTATCTACGGAAAATTTCTCTGAAGATGATCACTATGGAGACCAAGTCTCAGAACACAATGGCCAATTCTTTACAATCCAACTCTGCTGGTAATACCTATGAAACATGTGATAGTAATTCATGCAACTCCATCTTATTGTTTTTTATTTTTTCATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

351

Amino Acids

40.03

Weight (kDa)

8.85

Isoelectric Point (pI)

65.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 242 - 322 5.6e-32 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 312, 634
Acc36I ACCTGC 2 cut(s) 312, 634
AccII CGCG 1 cut(s) 757
AccIII TCCGGA 1 cut(s) 595
AciI CCGC 3 cut(s) 80, 749, 757
AclWI GGATC 1 cut(s) 792
AcoI YGGCCR 1 cut(s) 957
AcsI RAATTY 3 cut(s) 226, 843, 909
AcuI CTGAAG 2 cut(s) 857, 938
AfaI GTAC 2 cut(s) 68, 677
AfiI CCNNNNNNNGG 4 cut(s) 365, 698, 701, 745
AflIII ACRYGT 1 cut(s) 1001
AgsI TTSAA 5 cut(s) 190, 411, 419, 539, 793
AjnI CCWGG 3 cut(s) 60, 181, 248
AjuI GAANNNNNNNTTGG 2 cut(s) 182, 214
AluBI AGCT 5 cut(s) 103, 112, 427, 559, 778
AluI AGCT 5 cut(s) 103, 112, 427, 559, 778
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 5 cut(s) 2, 381, 593, 929, 948
AlwI GGATC 1 cut(s) 792
Aor13HI TCCGGA 1 cut(s) 595
AoxI GGCC 2 cut(s) 653, 957
ApeKI GCWGC 5 cut(s) 307, 493, 527, 559, 878
ApoI RAATTY 3 cut(s) 226, 843, 909
AseI ATTAAT 1 cut(s) 234
Asp700I GAANNNNTTC 1 cut(s) 36
AspS9I GGNCC 1 cut(s) 126
AsuHPI GGTGA 2 cut(s) 171, 615
AvaII GGWCC 1 cut(s) 126
AxyI CCTNAGG 1 cut(s) 318
BalI TGGCCA 1 cut(s) 959
BarI GAAGNNNNNNTAC 1 cut(s) 28
BbsI GAAGAC 1 cut(s) 260
Bbv12I GWGCWC 1 cut(s) 141
BbvI GCAGC 5 cut(s) 294, 505, 539, 546, 865
BccI CCATC 3 cut(s) 397, 677, 1034
BciT130I CCWGG 3 cut(s) 62, 183, 250
BclI TGATCA 1 cut(s) 924
BcoDI GTCTC 5 cut(s) 2, 381, 593, 929, 948
BfaI CTAG 1 cut(s) 617
BfuAI ACCTGC 2 cut(s) 312, 634
BisI GCNGC 6 cut(s) 308, 494, 528, 560, 758, 879
BlsI GCNGC 6 cut(s) 309, 495, 529, 561, 759, 880
Bme1390I CCNGG 3 cut(s) 62, 183, 250
Bme18I GGWCC 1 cut(s) 126
BmgT120I GGNCC 1 cut(s) 126
BmiI GGNNCC 1 cut(s) 737
BmrFI CCNGG 3 cut(s) 62, 183, 250
BmsI GCATC 3 cut(s) 353, 518, 808
BoxI GACNNNNGTC 1 cut(s) 373
BpiI GAAGAC 1 cut(s) 260
BplI GAGNNNNNCTC 2 cut(s) 679, 711
BpmI CTGGAG 2 cut(s) 44, 796
BpuEI CTTGAG 3 cut(s) 125, 348, 681
BsaI GGTCTC 1 cut(s) 929
BsaJI CCNNGG 1 cut(s) 248
BsaWI WCCGGW 1 cut(s) 595
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 926, 956
Bsc4I CCNNNNNNNGG 4 cut(s) 365, 698, 701, 745
Bse1I ACTGG 3 cut(s) 568, 640, 813
Bse21I CCTNAGG 1 cut(s) 318
Bse3DI GCAATG 1 cut(s) 720
BseAI TCCGGA 1 cut(s) 595
BseBI CCWGG 3 cut(s) 62, 183, 250
BseDI CCNNGG 1 cut(s) 248
BseGI GGATG 2 cut(s) 385, 724
BseLI CCNNNNNNNGG 4 cut(s) 365, 698, 701, 745
BseMI GCAATG 1 cut(s) 720
BseMII CTCAG 2 cut(s) 437, 959
BseNI ACTGG 3 cut(s) 568, 640, 813
BseRI GAGGAG 2 cut(s) 684, 748
BseXI GCAGC 5 cut(s) 294, 505, 539, 546, 865
Bsh1236I CGCG 1 cut(s) 757
BshFI GGCC 2 cut(s) 655, 959
BsiHKAI GWGCWC 1 cut(s) 141
BsiSI CCGG 1 cut(s) 596
BslI CCNNNNNNNGG 4 cut(s) 365, 698, 701, 745
BsmAI GTCTC 5 cut(s) 2, 381, 593, 929, 948
BsnI GGCC 2 cut(s) 655, 959
Bso31I GGTCTC 1 cut(s) 929
Bsp1286I GDGCHC 1 cut(s) 141
Bsp13I TCCGGA 1 cut(s) 595
Bsp143I GATC 4 cut(s) 219, 334, 784, 924
BspACI CCGC 3 cut(s) 80, 749, 757
BspANI GGCC 2 cut(s) 655, 959
BspCNI CTCAG 2 cut(s) 436, 958
BspEI TCCGGA 1 cut(s) 595
BspFNI CGCG 1 cut(s) 757
BspHI TCATGA 1 cut(s) 669
BspLI GGNNCC 1 cut(s) 737
BspMI ACCTGC 2 cut(s) 312, 634
BspPI GGATC 1 cut(s) 792
BspTNI GGTCTC 1 cut(s) 929
BsrDI GCAATG 1 cut(s) 720
BsrI ACTGG 3 cut(s) 568, 640, 813
BssECI CCNNGG 1 cut(s) 248
BssMI GATC 4 cut(s) 219, 334, 784, 924
Bst2UI CCWGG 3 cut(s) 62, 183, 250
Bst4CI ACNGT 5 cut(s) 87, 151, 259, 450, 468
Bst6I CTCTTC 1 cut(s) 788
BstAPI GCANNNNNTGC 1 cut(s) 490
BstC8I GCNNGC 3 cut(s) 458, 711, 747
BstDEI CTNAG 3 cut(s) 318, 423, 945
BstENI CCTNNNNNAGG 2 cut(s) 363, 696
BstF5I GGATG 2 cut(s) 385, 724
BstFNI CGCG 1 cut(s) 757
BstKTI GATC 4 cut(s) 222, 337, 787, 927
BstMAI GTCTC 5 cut(s) 2, 381, 593, 929, 948
BstMBI GATC 4 cut(s) 219, 334, 784, 924
BstMWI GCNNNNNNNGC 3 cut(s) 109, 490, 499
BstNI CCWGG 3 cut(s) 62, 183, 250
BstNSI RCATGY 2 cut(s) 460, 1005
BstPAI GACNNNNGTC 1 cut(s) 373
BstSCI CCNGG 3 cut(s) 60, 181, 248
BstUI CGCG 1 cut(s) 757
BstV1I GCAGC 5 cut(s) 294, 505, 539, 546, 865
BstV2I GAAGAC 1 cut(s) 260
BstX2I RGATCY 1 cut(s) 784
BstYI RGATCY 1 cut(s) 784
Bsu36I CCTNAGG 1 cut(s) 318
BsuRI GGCC 2 cut(s) 655, 959
BtsCI GGATG 2 cut(s) 385, 724
BveI ACCTGC 2 cut(s) 312, 634
Cac8I GCNNGC 3 cut(s) 458, 711, 747
CciI TCATGA 1 cut(s) 669
Cfr13I GGNCC 1 cut(s) 126
CsiI ACCWGGT 1 cut(s) 181
Csp6I GTAC 2 cut(s) 67, 676
CviAII CATG 5 cut(s) 130, 457, 670, 1002, 1017
CviQI GTAC 2 cut(s) 67, 676
DdeI CTNAG 3 cut(s) 318, 423, 945
DpnI GATC 4 cut(s) 221, 336, 786, 926
DpnII GATC 4 cut(s) 219, 334, 784, 924
EaeI YGGCCR 1 cut(s) 957
Eam1104I CTCTTC 1 cut(s) 788
EarI CTCTTC 1 cut(s) 788
EciI GGCGGA 2 cut(s) 69, 764
Eco147I AGGCCT 1 cut(s) 655
Eco31I GGTCTC 1 cut(s) 929
Eco47I GGWCC 1 cut(s) 126
Eco57I CTGAAG 2 cut(s) 857, 938
Eco81I CCTNAGG 1 cut(s) 318
EcoNI CCTNNNNNAGG 2 cut(s) 363, 696
EcoRII CCWGG 3 cut(s) 60, 181, 248
FaeI CATG 5 cut(s) 133, 460, 673, 1005, 1020
FatI CATG 5 cut(s) 129, 456, 669, 1001, 1016
FbaI TGATCA 1 cut(s) 924
Fnu4HI GCNGC 6 cut(s) 308, 494, 528, 560, 758, 879
FokI GGATG 2 cut(s) 372, 731
Fsp4HI GCNGC 6 cut(s) 308, 494, 528, 560, 758, 879
FspBI CTAG 1 cut(s) 617
GluI GCNGC 6 cut(s) 308, 494, 528, 560, 758, 879
GsuI CTGGAG 2 cut(s) 44, 796
HaeIII GGCC 2 cut(s) 655, 959
HapII CCGG 1 cut(s) 596
Hin1II CATG 5 cut(s) 133, 460, 673, 1005, 1020
HindIII AAGCTT 2 cut(s) 101, 776
HinfI GANTC 4 cut(s) 71, 620, 688, 752
HpaII CCGG 1 cut(s) 596
HphI GGTGA 2 cut(s) 171, 615
Hpy188I TCNGA 5 cut(s) 42, 339, 895, 918, 948
Hpy188III TCNNGA 5 cut(s) 95, 596, 617, 670, 818
HpyAV CCTTC 1 cut(s) 184
HpyCH4III ACNGT 5 cut(s) 87, 151, 259, 450, 468
HpyF10VI GCNNNNNNNGC 3 cut(s) 109, 490, 499
HpyF3I CTNAG 3 cut(s) 318, 423, 945
Hsp92II CATG 5 cut(s) 133, 460, 673, 1005, 1020
Kpn2I TCCGGA 1 cut(s) 595
Ksp22I TGATCA 1 cut(s) 924
Kzo9I GATC 4 cut(s) 219, 334, 784, 924
LmnI GCTCC 4 cut(s) 136, 325, 556, 735
Lsp1109I GCAGC 5 cut(s) 294, 505, 539, 546, 865
LweI GCATC 3 cut(s) 353, 518, 808
MabI ACCWGGT 1 cut(s) 181
MaeI CTAG 1 cut(s) 617
MalI GATC 4 cut(s) 221, 336, 786, 926
MboI GATC 4 cut(s) 219, 334, 784, 924
MboII GAAGA 7 cut(s) 255, 265, 266, 794, 805, 850, 931
MfeI CAATTG 1 cut(s) 740
MflI RGATCY 1 cut(s) 784
MhlI GDGCHC 1 cut(s) 141
MlsI TGGCCA 1 cut(s) 959
MluNI TGGCCA 1 cut(s) 959
MlyI GAGTC 2 cut(s) 80, 697
MmeI TCCRAC 2 cut(s) 114, 999
MnlI CCTC 9 cut(s) 152, 393, 645, 702, 705, 726, 789, 814, 853
Mox20I TGGCCA 1 cut(s) 959
MroI TCCGGA 1 cut(s) 595
MroXI GAANNNNTTC 1 cut(s) 36
MscI TGGCCA 1 cut(s) 959
MseI TTAA 5 cut(s) 26, 234, 353, 470, 1054
Msp20I TGGCCA 1 cut(s) 959
MspI CCGG 1 cut(s) 596
MspR9I CCNGG 3 cut(s) 62, 183, 250
MunI CAATTG 1 cut(s) 740
MvaI CCWGG 3 cut(s) 62, 183, 250
MvnI CGCG 1 cut(s) 757
MwoI GCNNNNNNNGC 3 cut(s) 109, 490, 499
NdeII GATC 4 cut(s) 219, 334, 784, 924
NlaIII CATG 5 cut(s) 133, 460, 673, 1005, 1020
NlaIV GGNNCC 1 cut(s) 737
NspI RCATGY 2 cut(s) 460, 1005
PaeI GCATGC 1 cut(s) 460
PagI TCATGA 1 cut(s) 669
PaqCI CACCTGC 2 cut(s) 312, 634
PceI AGGCCT 1 cut(s) 655
PciI ACATGT 1 cut(s) 1001
PdmI GAANNNNTTC 1 cut(s) 36
PfeI GAWTC 2 cut(s) 620, 752
PflFI GACNNNGTC 1 cut(s) 940
PfoI TCCNGGA 1 cut(s) 60
PkrI GCNGC 6 cut(s) 309, 495, 529, 561, 759, 880
PleI GAGTC 2 cut(s) 79, 696
PpsI GAGTC 2 cut(s) 79, 696
PscI ACATGT 1 cut(s) 1001
PshAI GACNNNNGTC 1 cut(s) 373
PshBI ATTAAT 1 cut(s) 234
Psp6I CCWGG 3 cut(s) 60, 181, 248
PspGI CCWGG 3 cut(s) 60, 181, 248
PspN4I GGNNCC 1 cut(s) 737
PspPI GGNCC 1 cut(s) 126
PsuI RGATCY 1 cut(s) 784
PsyI GACNNNGTC 1 cut(s) 940
RsaI GTAC 2 cut(s) 68, 677
RsaNI GTAC 2 cut(s) 67, 676
SaqAI TTAA 5 cut(s) 26, 234, 353, 470, 1054
SatI GCNGC 6 cut(s) 308, 494, 528, 560, 758, 879
Sau3AI GATC 4 cut(s) 219, 334, 784, 924
Sau96I GGNCC 1 cut(s) 126
SchI GAGTC 2 cut(s) 80, 697
ScrFI CCNGG 3 cut(s) 62, 183, 250
SduI GDGCHC 1 cut(s) 141
SexAI ACCWGGT 1 cut(s) 181
SfaNI GCATC 3 cut(s) 353, 518, 808
SinI GGWCC 1 cut(s) 126
SmlI CTYRAG 3 cut(s) 104, 363, 696
SmoI CTYRAG 3 cut(s) 104, 363, 696
SphI GCATGC 1 cut(s) 460
SseBI AGGCCT 1 cut(s) 655
SsiI CCGC 3 cut(s) 80, 749, 757
SspI AATATT 2 cut(s) 175, 415
SspMI CTAG 1 cut(s) 617
StuI AGGCCT 1 cut(s) 655
StyD4I CCNGG 3 cut(s) 60, 181, 248
TaaI ACNGT 5 cut(s) 87, 151, 259, 450, 468
TatI WGTACW 1 cut(s) 675
TauI GCSGC 1 cut(s) 760
TfiI GAWTC 2 cut(s) 620, 752
Tru1I TTAA 5 cut(s) 26, 234, 353, 470, 1054
Tru9I TTAA 5 cut(s) 26, 234, 353, 470, 1054
TseI GCWGC 5 cut(s) 307, 493, 527, 559, 878
TspDTI ATGAA 7 cut(s) 17, 220, 658, 686, 1005, 1011, 1038
TspGWI ACGGA 1 cut(s) 919
Tth111I GACNNNGTC 1 cut(s) 940
VpaK11BI GGWCC 1 cut(s) 126
VspI ATTAAT 1 cut(s) 234
XagI CCTNNNNNAGG 2 cut(s) 363, 696
XapI RAATTY 3 cut(s) 226, 843, 909
XbaI TCTAGA 1 cut(s) 616
XceI RCATGY 2 cut(s) 460, 1005
XmnI GAANNNNTTC 1 cut(s) 36
XspI CTAG 1 cut(s) 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.