Prupe.I003700_v2.0.a1

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
scaffold_271
OG0001201
Physical Location & Seq
Forward (+)
54 .. 2967
2914 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.I003700.1

Sequence Viewer

Length: 903 bp
ATGAACTTGCAAGGTTCTGTAGCAACAATGCCGCAGAGCAATATGACAAGCTTGCAGCAAAGTTCTATGTCGGCTTTATCTGGGGTTTCAACAGCGCAGCAGAACATGATGAATTCATTGCCACCTAGTTCCAGCATGGATTCAGGACAAGGGAATGCACTGAACTCATTGCAGCAGGTTCCTGTGGGAAGTAACCAACAAACTCCCGTAAGTGCTCCCCAACAAGCAAATATGAATGCCTTGTCATCACAGAGTGGGGTTAATATGCTACAGGCAAACATGAATTCCATTCAGTCAAATTCTGGTATGCTTCAACACCAGCATTTGAAACAACAGCAGGAACATCAAATGTTTCAGAATCAACTCAAGCAACAATTTCAACATCGACAGATGCAGCAGCAATTGATGCAGAAGCAGCAGTTACTGCAACATCAACAGCAGCAGCTGCAGCAGTTACAACTGCAAGCAGAGCAGCAGCTTCCAGCACAGTTGCAGGCGCACCAACAGCAAATGCCACAGCTTCATCAAATGAATGATGTAAATGACTTGAAGATGAGACAGGGGATGGGTGTAAAGCCAGGGGTTTTTCAGCAACATCTCTCTGTAGATAGTTGGTTTGTGATGCAAACAGGCTTCTTAGTTATCAAACTCATGAAGGAGATGTACTTACCCGAAAGTGAAAAAATTGCTACTAAACTTCTGCAGCAGCTTGACAAACTGAAAATGTTCAGGACCATGTTGGAGCGCCTCATATCAGTCTTACAGATTTCCAAAAGTAGCATTTCACCCGGTTTGAAGGACAAGTTGTTTTTTCACGAAAAGCAGATAGTAAATTTTATTAATGCCTACGAAGCCAGTTTCTGCAAGGCATCAATGGACAGCACAGCCGCCCCTCCTACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

301

Amino Acids

33.82

Weight (kDa)

9.35

Isoelectric Point (pI)

64.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 166
AciI CCGC 2 cut(s) 32, 888
AcsI RAATTY 4 cut(s) 112, 283, 298, 832
AdeI CACNNNGTG 1 cut(s) 254
AfaI GTAC 1 cut(s) 665
AgsI TTSAA 6 cut(s) 90, 314, 328, 380, 550, 796
AjnI CCWGG 1 cut(s) 577
AluBI AGCT 5 cut(s) 51, 445, 478, 520, 709
AluI AGCT 5 cut(s) 51, 445, 478, 520, 709
Alw21I GWGCWC 1 cut(s) 217
Alw26I GTCTC 1 cut(s) 550
AlwNI CAGNNNCTG 3 cut(s) 424, 445, 861
ApoI RAATTY 4 cut(s) 112, 283, 298, 832
AseI ATTAAT 1 cut(s) 840
Asp700I GAANNNNTTC 1 cut(s) 725
AspLEI GCGC 3 cut(s) 97, 499, 747
AspS9I GGNCC 1 cut(s) 732
AsuC2I CCSGG 1 cut(s) 789
AsuHPI GGTGA 1 cut(s) 777
AvaII GGWCC 1 cut(s) 732
BarI GAAGNNNNNNTAC 2 cut(s) 647, 679
Bbv12I GWGCWC 1 cut(s) 217
BccI CCATC 1 cut(s) 559
BciT130I CCWGG 1 cut(s) 579
BcnI CCSGG 1 cut(s) 789
BcoDI GTCTC 1 cut(s) 550
BfaI CTAG 1 cut(s) 126
BfmI CTRYAG 5 cut(s) 18, 269, 446, 603, 701
BfoI RGCGCY 1 cut(s) 748
BfuAI ACCTGC 1 cut(s) 166
Bme1390I CCNGG 2 cut(s) 579, 789
Bme18I GGWCC 1 cut(s) 732
BmgT120I GGNCC 1 cut(s) 732
BmiI GGNNCC 1 cut(s) 180
BmrFI CCNGG 2 cut(s) 579, 789
BmsI GCATC 4 cut(s) 381, 396, 612, 878
BpuEI CTTGAG 1 cut(s) 350
BpuMI CCSGG 1 cut(s) 789
BsaJI CCNNGG 1 cut(s) 578
Bse1I ACTGG 1 cut(s) 855
Bse3DI GCAATG 2 cut(s) 116, 167
BseBI CCWGG 1 cut(s) 579
BseDI CCNNGG 1 cut(s) 578
BseGI GGATG 1 cut(s) 570
BseMI GCAATG 2 cut(s) 116, 167
BseNI ACTGG 1 cut(s) 855
BsiHKAI GWGCWC 1 cut(s) 217
BsiSI CCGG 1 cut(s) 789
BsmAI GTCTC 1 cut(s) 550
BsmI GAATGC 2 cut(s) 160, 241
Bsp1286I GDGCHC 1 cut(s) 217
BspACI CCGC 2 cut(s) 32, 888
BspHI TCATGA 1 cut(s) 651
BspLI GGNNCC 1 cut(s) 180
BspMAI CTGCAG 2 cut(s) 450, 705
BspMI ACCTGC 1 cut(s) 166
BsrDI GCAATG 2 cut(s) 116, 167
BsrI ACTGG 1 cut(s) 855
BssECI CCNNGG 1 cut(s) 578
Bst2UI CCWGG 1 cut(s) 579
Bst4CI ACNGT 1 cut(s) 489
BstAPI GCANNNNNTGC 3 cut(s) 406, 424, 445
BstC8I GCNNGC 3 cut(s) 53, 465, 495
BstDEI CTNAG 1 cut(s) 637
BstF5I GGATG 1 cut(s) 570
BstH2I RGCGCY 1 cut(s) 748
BstHHI GCGC 3 cut(s) 97, 499, 747
BstMAI GTCTC 1 cut(s) 550
BstMWI GCNNNNNNNGC 8 cut(s) 406, 415, 424, 445, 448, 469, 505, 851
BstNI CCWGG 1 cut(s) 579
BstSCI CCNGG 2 cut(s) 577, 787
BstSFI CTRYAG 5 cut(s) 18, 269, 446, 603, 701
BtsCI GGATG 1 cut(s) 570
BtsIMutI CAGTG 1 cut(s) 158
BveI ACCTGC 1 cut(s) 166
Cac8I GCNNGC 3 cut(s) 53, 465, 495
CaiI CAGNNNCTG 3 cut(s) 424, 445, 861
CciI TCATGA 1 cut(s) 651
CfoI GCGC 3 cut(s) 97, 499, 747
Cfr13I GGNCC 1 cut(s) 732
Csp6I GTAC 1 cut(s) 664
CviAII CATG 5 cut(s) 106, 136, 280, 652, 736
CviQI GTAC 1 cut(s) 664
DdeI CTNAG 1 cut(s) 637
DraIII CACNNNGTG 1 cut(s) 254
Eco47I GGWCC 1 cut(s) 732
EcoRI GAATTC 2 cut(s) 112, 283
EcoRII CCWGG 1 cut(s) 577
FaeI CATG 5 cut(s) 109, 139, 283, 655, 739
FatI CATG 5 cut(s) 105, 135, 279, 651, 735
FokI GGATG 1 cut(s) 577
FspBI CTAG 1 cut(s) 126
GlaI GCGC 3 cut(s) 96, 498, 746
HaeII RGCGCY 1 cut(s) 748
HapII CCGG 1 cut(s) 789
HhaI GCGC 3 cut(s) 97, 499, 747
Hin1II CATG 5 cut(s) 109, 139, 283, 655, 739
Hin6I GCGC 3 cut(s) 95, 497, 745
HinP1I GCGC 3 cut(s) 95, 497, 745
HindIII AAGCTT 1 cut(s) 49
HinfI GANTC 2 cut(s) 140, 358
HpaII CCGG 1 cut(s) 789
HphI GGTGA 1 cut(s) 777
Hpy188I TCNGA 1 cut(s) 357
Hpy188III TCNNGA 4 cut(s) 144, 652, 730, 815
HpyAV CCTTC 2 cut(s) 649, 790
HpyCH4III ACNGT 1 cut(s) 489
HpyF10VI GCNNNNNNNGC 8 cut(s) 406, 415, 424, 445, 448, 469, 505, 851
HpyF3I CTNAG 1 cut(s) 637
Hsp92II CATG 5 cut(s) 109, 139, 283, 655, 739
HspAI GCGC 3 cut(s) 95, 497, 745
LmnI GCTCC 2 cut(s) 220, 742
LweI GCATC 4 cut(s) 381, 396, 612, 878
MaeI CTAG 1 cut(s) 126
MaeIII GTNAC 3 cut(s) 191, 420, 453
MboII GAAGA 1 cut(s) 562
MfeI CAATTG 1 cut(s) 401
MhlI GDGCHC 1 cut(s) 217
MluCI AATT 7 cut(s) 112, 283, 298, 374, 401, 684, 832
MmeI TCCRAC 1 cut(s) 720
MnlI CCTC 2 cut(s) 758, 903
MroXI GAANNNNTTC 1 cut(s) 725
MseI TTAA 2 cut(s) 261, 840
MspA1I CMGCKG 1 cut(s) 445
MspI CCGG 1 cut(s) 789
MspR9I CCNGG 2 cut(s) 579, 789
MunI CAATTG 1 cut(s) 401
Mva1269I GAATGC 2 cut(s) 160, 241
MvaI CCWGG 1 cut(s) 579
MwoI GCNNNNNNNGC 8 cut(s) 406, 415, 424, 445, 448, 469, 505, 851
NciI CCSGG 1 cut(s) 789
NlaIII CATG 5 cut(s) 109, 139, 283, 655, 739
NlaIV GGNNCC 1 cut(s) 180
PagI TCATGA 1 cut(s) 651
PctI GAATGC 2 cut(s) 160, 241
PdmI GAANNNNTTC 1 cut(s) 725
PfeI GAWTC 2 cut(s) 140, 358
PshBI ATTAAT 1 cut(s) 840
Psp6I CCWGG 1 cut(s) 577
PspGI CCWGG 1 cut(s) 577
PspN4I GGNNCC 1 cut(s) 180
PspPI GGNCC 1 cut(s) 732
PstI CTGCAG 2 cut(s) 450, 705
PstNI CAGNNNCTG 3 cut(s) 424, 445, 861
PvuII CAGCTG 1 cut(s) 445
RsaI GTAC 1 cut(s) 665
RsaNI GTAC 1 cut(s) 664
SaqAI TTAA 2 cut(s) 261, 840
Sau96I GGNCC 1 cut(s) 732
ScrFI CCNGG 2 cut(s) 579, 789
SduI GDGCHC 1 cut(s) 217
SetI ASST 9 cut(s) 16, 53, 127, 180, 447, 480, 522, 711, 902
SfaNI GCATC 4 cut(s) 381, 396, 612, 878
SfcI CTRYAG 5 cut(s) 18, 269, 446, 603, 701
SinI GGWCC 1 cut(s) 732
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
Sse9I AATT 7 cut(s) 112, 283, 298, 374, 401, 684, 832
SsiI CCGC 2 cut(s) 32, 888
SspMI CTAG 1 cut(s) 126
StyD4I CCNGG 2 cut(s) 577, 787
TaaI ACNGT 1 cut(s) 489
TaqI TCGA 1 cut(s) 385
TasI AATT 7 cut(s) 112, 283, 298, 374, 401, 684, 832
TatI WGTACW 1 cut(s) 663
TauI GCSGC 2 cut(s) 34, 890
TfiI GAWTC 2 cut(s) 140, 358
Tru1I TTAA 2 cut(s) 261, 840
Tru9I TTAA 2 cut(s) 261, 840
TscAI CASTG 1 cut(s) 165
TspDTI ATGAA 8 cut(s) 17, 105, 125, 248, 296, 512, 545, 668
TspRI CASTG 1 cut(s) 165
VpaK11BI GGWCC 1 cut(s) 732
VspI ATTAAT 1 cut(s) 840
XapI RAATTY 4 cut(s) 112, 283, 298, 832
XmnI GAANNNNTTC 1 cut(s) 725
XspI CTAG 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.