Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0001201

Physical Location & Seq

Genomic Coordinates Forward (+)

82181146 .. 82189979

Gene Structure 8834 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG533300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1998 bp

ATGGATCAGAAGAAGCAGTGGTATCAATCTCGAAGAGCCCTTCCGGAGAGTTCAACAAATTCCACAACACATACAAATAGAGGTGATTGGCAAGAGGAGGTCTATCAAAAGATCATAGTCATGAAGGAGAAGTACTTGCCTGAACTAAGTGAAATGTACCAGAAAAGTGCTAATAAACTTCAGCAGCACGAGTCTCTTCCGCAGCCACCAAATTCAGATTCTCTTGCGAAGCTGAAAATGTTCAAGTCCATGCTGGACCGCCTCATAACAGTCCTACAGGTGTCCAAAAGTAACATTTCACCTGGTTTGAAGGAGAAGTTGGGTATATACGAGAAGCAGATTATAAATTTCATTAATACAAATAGACCCAGGAAGACTGTTTCTTCTCTACATCTTCCCCCACCTGAGATGCACAATGCACACCTGCTGCAACAACCCAAGATGGAGCAACAAGATTCAAATGATCATGTAATATATCTTAATTTCTTATCCCCGACTAGTGATACAAGTGGCACTGATTGGCAAGAGGAGGTCTATCAAAAGATCCAAGTCATGAAGGAGACTTACTTGCCTGAATTAAGTGAAATGTTTCAGAAAATTGATAATAAACTCCAGGAGTACGAGTCTGTTCCGCCACAGTCAAATCCAGATAAGCTTGAGAAGCTGAAAGTGTTTAGGACCATGTTGGAGCACCTCATAACAGTCTTACAGGTTTCCAAAAGTAATATTTCACCTGGTTTGAAGGAGAAGTTGGGTTTATACGAGAAGCAGATTATAAATTTCATTAATACAAATAGACCCAGGAAGACTGTTTCTTCTCTACATCTTCCCCCACCTGATATGCACAATGCACACCTGCTGCAACAACCTAAGGTGGAGCAACATGATCAGAGTGCATCTAACTTAATACCTACTCAAGGACAGCAGTCTCAACCTCATCCATCACAGCAACAATATCTTTCAAAGATTCAATCTCAGCTTACACATATACAACAACAACAGTTGAGCATGCAACAACAGTTAATCCCACTACTGCAAGATATGCAGCAAAGGCTCGAAGCATCAGGTCACGTATCTGCAGCCATACTTCAACCACCAAATACAATGGAGCTGCAAAACCAGTTATCAAGTATAGCCCTTCTGGAGACGTCATCAACATCTGTAGATTCACCTGCCCTGACTGGACATACAGACAAGAGGCCTATCAAAAGGTTCATGAAGTACAATGATGGGAGTCCTCCTCAAGGTGGAGAGCCTGCAATGGATGCAGGAGATTGGAGGAGCCAATTACAGGCGGATTCGCGGCAAAGAATTGTCAATAAGCTTATGGATGCATTGAAGAGGCATCTTCCTTTCTGCGGTCAAGAAGGATTGGATGAACTCAAGAAAATCGCTGTTAGATTTGAGGAAAAGATTTATGTTGCTGCCACAAGTCAGACAGATTATCTACGGAAAATTTCTCTGAAGATGATCACTTTGGAGACCAAGTCTCAGAAAACAATGGCCGATTCTTTACAATCCAACTCTGCTGCATCTCTAGATTCACCTGCCCTGACTGGACATACAGAGAAGAGGCCTATCAAACGGTTCAAGAAGTACAACGATGGGAGTCCCCCTCAAGGTGGAGAGCCTGCAATGGATGCAGGAGACTGGAGGAGCCAATTGCAGGCGGATTCGCGGCAAAGAATTGTCAATAAGCTGATGGATACATTGAAGAGGCATCTTCCTTTCTCCAGTCAAGAGGGATTGGATGAACTCAAGAAAATTGCTGTTAGATTTGAGGAAAAGATTTATGGTGCTGCCACAAGTCAGTCAGATTATCTACGGAAAATTTCTCTGAAGATGATCACTATGGAGACCAAGTCTCAGAACACAATGGCCAATTCTTTACAATCCAACTCTGCTGGTAATACCTATGAAACATATGTGATGCAGAACGGATGGCAGTCCAATTCGAAGAGCAGCTTGATCGTGCAAGAGGAGGAAAACGAAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KIX_2

Protein Analysis

665

Amino Acids

75.9

Weight (kDa)

8.9

Isoelectric Point (pI)

62.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KIX_2	PF16987	424 - 502	1.8e-31	KIX domain
KIX_2	PF16987	549 - 628	7.3e-34	KIX domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001201)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15772 AT1G15772 AT1G15772 AT1G15772
fragaria_vesca	FvH4_3g37510 FvH4_3g37510 FvH4_3g37510 FvH4_3g37510
malus_domestica	MD02G1272400.v1.1 MD03G1032800.v1.1 MD03G1033000.v1.1 MD03G1033200.v1.1 MD03G1033300.v1.1 MD03G1033600.v1.1 MD03G1033800.v1.1 MD03G1034600.v1.1
prunus_persica	Prupe.6G027600_v2.0.a1 Prupe.6G027900_v2.0.a1 Prupe.6G027900_v2.0.a1 Prupe.6G027900_v2.0.a1 Prupe.6G027900_v2.0.a1 Prupe.6G027900_v2.0.a1 Prupe.6G027900_v2.0.a1 Prupe.6G027900_v2.0.a1 Prupe.6G027900_v2.0.a1 Prupe.6G027900_v2.0.a1 Prupe.6G027900_v2.0.a1 Prupe.6G027900_v2.0.a1 Prupe.6G027900_v2.0.a1 Prupe.6G027900_v2.0.a1 Prupe.6G027900_v2.0.a1 Prupe.6G027900_v2.0.a1 Prupe.6G027900_v2.0.a1 Prupe.6G027900_v2.0.a1 Prupe.6G027900_v2.0.a1 Prupe.6G027900_v2.0.a1 Prupe.6G027900_v2.0.a1 Prupe.6G027900_v2.0.a1 Prupe.6G027900_v2.0.a1 Prupe.I003700_v2.0.a1
pyrus_communis	pycom02g23320 pycom03g02630 pycom03g02650 pycom03g02660
rosa_chinensis	RchiOBHm_Chr5g0075821
rosa_laevigata	RLG00000036593 RLG00000036596
rosa_multiflora	Rmu_co8383039.1_g000001
rosa_roxburghii	Rroxscaffold_2G00082370
rosa_rugosa	Rorug05G0442800
rosa_samantha	Rh5BG520700 Rh5BG521000 Rh5CG544700 Rh5CG545100 Rh5DG533300
rosa_wichuraiana	Rw5G046390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	344, 776
AarI	CACCTGC	4 cut(s)	432, 864, 1180, 1555
AatII	GACGTC	1 cut(s)	1151
Acc36I	ACCTGC	4 cut(s)	432, 864, 1180, 1555
AccII	CGCG	2 cut(s)	1303, 1678
AccIII	TCCGGA	1 cut(s)	43
AciI	CCGC	8 cut(s)	200, 259, 632, 1295, 1303, 1359, 1670, 1678
AclWI	GGATC	2 cut(s)	12, 538
AcoI	YGGCCR	2 cut(s)	1503, 1878
AcsI	RAATTY	6 cut(s)	58, 211, 346, 778, 1455, 1830
AcuI	CTGAAG	3 cut(s)	164, 1484, 1859
AcyI	GRCGYC	1 cut(s)	1148
AfaI	GTAC	5 cut(s)	134, 158, 620, 1223, 1598
AfiI	CCNNNNNNNGG	8 cut(s)	917, 1244, 1247, 1291, 1358, 1619, 1622, 1666
AhlI	ACTAGT	1 cut(s)	497
AjnI	CCWGG	5 cut(s)	301, 368, 612, 733, 800
AjuI	GAANNNNNNNTTGG	6 cut(s)	202, 234, 302, 334, 734, 766
AluBI	AGCT	8 cut(s)	232, 655, 664, 979, 1111, 1324, 1699, 1965
AluI	AGCT	8 cut(s)	232, 655, 664, 979, 1111, 1324, 1699, 1965
Alw21I	GWGCWC	1 cut(s)	693
Alw26I	GTCTC	9 cut(s)	198, 554, 933, 1139, 1475, 1494, 1641, 1850, 1869
AlwI	GGATC	2 cut(s)	12, 538
Aor13HI	TCCGGA	1 cut(s)	43
AoxI	GGCC	4 cut(s)	1199, 1503, 1574, 1878
ApoI	RAATTY	6 cut(s)	58, 211, 346, 778, 1455, 1830
AseI	ATTAAT	2 cut(s)	354, 786
Asp700I	GAANNNNTTC	2 cut(s)	239, 588
AspS9I	GGNCC	2 cut(s)	256, 678
AsuHPI	GGTGA	5 cut(s)	95, 291, 723, 1161, 1536
AsuII	TTCGAA	1 cut(s)	1955
AvaII	GGWCC	2 cut(s)	256, 678
AxyI	CCTNAGG	1 cut(s)	870
BalI	TGGCCA	1 cut(s)	1880
BanII	GRGCYC	1 cut(s)	40
BarI	GAAGNNNNNNTAC	4 cut(s)	116, 148, 548, 580
BauI	CACGAG	1 cut(s)	188
BbsI	GAAGAC	2 cut(s)	380, 812
Bbv12I	GWGCWC	1 cut(s)	693
BccI	CCATC	6 cut(s)	436, 949, 1223, 1598, 1696, 1935
BcgI	CGANNNNNNTGC	2 cut(s)	1951, 1985
BciT130I	CCWGG	5 cut(s)	303, 370, 614, 735, 802
BciVI	GTATCC	1 cut(s)	1699
BclI	TGATCA	4 cut(s)	463, 886, 1470, 1845
BcoDI	GTCTC	9 cut(s)	198, 554, 933, 1139, 1475, 1494, 1641, 1850, 1869
BcuI	ACTAGT	1 cut(s)	497
BfaI	CTAG	2 cut(s)	498, 1538
BfmI	CTRYAG	3 cut(s)	275, 1077, 1161
BfuAI	ACCTGC	4 cut(s)	432, 864, 1180, 1555
BfuI	GTATCC	1 cut(s)	1699
BmcAI	AGTACT	1 cut(s)	134
Bme1390I	CCNGG	5 cut(s)	303, 370, 614, 735, 802
Bme18I	GGWCC	2 cut(s)	256, 678
BmgT120I	GGNCC	2 cut(s)	256, 678
BmiI	GGNNCC	2 cut(s)	1283, 1658
BmrFI	CCNGG	5 cut(s)	303, 370, 614, 735, 802
BoxI	GACNNNNGTC	1 cut(s)	925
BpiI	GAAGAC	2 cut(s)	380, 812
BplI	GAGNNNNNCTC	4 cut(s)	1225, 1257, 1600, 1632
BpmI	CTGGAG	4 cut(s)	596, 1163, 1672, 1717
Bpu14I	TTCGAA	1 cut(s)	1955
BpuEI	CTTGAG	6 cut(s)	677, 900, 1227, 1367, 1602, 1742
BsaAI	YACGTR	1 cut(s)	1072
BsaHI	GRCGYC	1 cut(s)	1148
BsaI	GGTCTC	2 cut(s)	1475, 1850
BsaJI	CCNNGG	2 cut(s)	368, 800
BsaWI	WCCGGW	1 cut(s)	43
BsaXI	ACNNNNNCTCC	6 cut(s)	608, 638, 1472, 1502, 1847, 1877
Bsc4I	CCNNNNNNNGG	8 cut(s)	917, 1244, 1247, 1291, 1358, 1619, 1622, 1666
Bse1I	ACTGG	5 cut(s)	1120, 1186, 1561, 1655, 1734
Bse21I	CCTNAGG	1 cut(s)	870
Bse3DI	GCAATG	2 cut(s)	1266, 1641
BseAI	TCCGGA	1 cut(s)	43
BseBI	CCWGG	5 cut(s)	303, 370, 614, 735, 802
BseDI	CCNNGG	2 cut(s)	368, 800
BseGI	GGATG	7 cut(s)	937, 1270, 1336, 1381, 1645, 1756, 1946
BseLI	CCNNNNNNNGG	8 cut(s)	917, 1244, 1247, 1291, 1358, 1619, 1622, 1666
BseMI	GCAATG	2 cut(s)	1266, 1641
BseMII	CTCAG	4 cut(s)	396, 989, 1505, 1880
BseNI	ACTGG	5 cut(s)	1120, 1186, 1561, 1655, 1734
BseRI	GAGGAG	6 cut(s)	110, 542, 1230, 1294, 1669, 1994
Bsh1236I	CGCG	2 cut(s)	1303, 1678
BshFI	GGCC	4 cut(s)	1201, 1505, 1576, 1880
BsiHKAI	GWGCWC	1 cut(s)	693
BsiSI	CCGG	1 cut(s)	44
BslFI	GGGAC	1 cut(s)	1596
BslI	CCNNNNNNNGG	8 cut(s)	917, 1244, 1247, 1291, 1358, 1619, 1622, 1666
BsmAI	GTCTC	9 cut(s)	198, 554, 933, 1139, 1475, 1494, 1641, 1850, 1869
BsmBI	CGTCTC	1 cut(s)	1139
BsmFI	GGGAC	1 cut(s)	1596
BsnI	GGCC	4 cut(s)	1201, 1505, 1576, 1880
Bso31I	GGTCTC	2 cut(s)	1475, 1850
Bsp119I	TTCGAA	1 cut(s)	1955
Bsp1286I	GDGCHC	2 cut(s)	40, 693
Bsp13I	TCCGGA	1 cut(s)	43
Bsp143I	GATC	8 cut(s)	4, 111, 463, 543, 886, 1470, 1845, 1968
BspACI	CCGC	8 cut(s)	200, 259, 632, 1295, 1303, 1359, 1670, 1678
BspANI	GGCC	4 cut(s)	1201, 1505, 1576, 1880
BspCNI	CTCAG	4 cut(s)	397, 988, 1504, 1879
BspEI	TCCGGA	1 cut(s)	43
BspFNI	CGCG	2 cut(s)	1303, 1678
BspHI	TCATGA	3 cut(s)	120, 552, 1215
BspLI	GGNNCC	2 cut(s)	1283, 1658
BspMAI	CTGCAG	1 cut(s)	1081
BspMI	ACCTGC	4 cut(s)	432, 864, 1180, 1555
BspPI	GGATC	2 cut(s)	12, 538
BspQI	GCTCTTC	2 cut(s)	28, 1952
BspT104I	TTCGAA	1 cut(s)	1955
BspTNI	GGTCTC	2 cut(s)	1475, 1850
BsrDI	GCAATG	2 cut(s)	1266, 1641
BsrI	ACTGG	5 cut(s)	1120, 1186, 1561, 1655, 1734
BssECI	CCNNGG	2 cut(s)	368, 800
BssMI	GATC	8 cut(s)	4, 111, 463, 543, 886, 1470, 1845, 1968
BssNI	GRCGYC	1 cut(s)	1148
BssSI	CACGAG	1 cut(s)	188
Bst2BI	CACGAG	1 cut(s)	188
Bst2UI	CCWGG	5 cut(s)	303, 370, 614, 735, 802
Bst4CI	ACNGT	8 cut(s)	271, 379, 639, 703, 811, 1002, 1020, 1587
Bst6I	CTCTTC	6 cut(s)	28, 201, 1334, 1565, 1709, 1952
BstACI	GRCGYC	1 cut(s)	1148
BstAPI	GCANNNNNTGC	3 cut(s)	1042, 1265, 1640
BstBAI	YACGTR	1 cut(s)	1072
BstBI	TTCGAA	1 cut(s)	1955
BstC8I	GCNNGC	4 cut(s)	1010, 1257, 1632, 1668
BstDEI	CTNAG	6 cut(s)	146, 405, 870, 975, 1491, 1866
BstENI	CCTNNNNNAGG	2 cut(s)	915, 1242
BstF5I	GGATG	7 cut(s)	937, 1270, 1336, 1381, 1645, 1756, 1946
BstFNI	CGCG	2 cut(s)	1303, 1678
BstKTI	GATC	8 cut(s)	7, 114, 466, 546, 889, 1473, 1848, 1971
BstMAI	GTCTC	9 cut(s)	198, 554, 933, 1139, 1475, 1494, 1641, 1850, 1869
BstMBI	GATC	8 cut(s)	4, 111, 463, 543, 886, 1470, 1845, 1968
BstMWI	GCNNNNNNNGC	5 cut(s)	661, 1042, 1051, 1265, 1640
BstNI	CCWGG	5 cut(s)	303, 370, 614, 735, 802
BstNSI	RCATGY	1 cut(s)	1012
BstPAI	GACNNNNGTC	1 cut(s)	925
BstSCI	CCNGG	5 cut(s)	301, 368, 612, 733, 800
BstSFI	CTRYAG	3 cut(s)	275, 1077, 1161
BstUI	CGCG	2 cut(s)	1303, 1678
BstV2I	GAAGAC	2 cut(s)	380, 812
BstX2I	RGATCY	1 cut(s)	543
BstYI	RGATCY	1 cut(s)	543
Bsu36I	CCTNAGG	1 cut(s)	870
BsuI	GTATCC	1 cut(s)	1699
BsuRI	GGCC	4 cut(s)	1201, 1505, 1576, 1880
BtsCI	GGATG	7 cut(s)	937, 1270, 1336, 1381, 1645, 1756, 1946
BtsI	GCAGTG	1 cut(s)	23
BtsIMutI	CAGTG	2 cut(s)	23, 513
BveI	ACCTGC	4 cut(s)	432, 864, 1180, 1555
Cac8I	GCNNGC	4 cut(s)	1010, 1257, 1632, 1668
CciI	TCATGA	3 cut(s)	120, 552, 1215
Cfr13I	GGNCC	2 cut(s)	256, 678
CsiI	ACCWGGT	2 cut(s)	301, 733
Csp6I	GTAC	5 cut(s)	133, 157, 619, 1222, 1597
CviAII	CATG	8 cut(s)	121, 250, 467, 553, 682, 884, 1009, 1216
CviQI	GTAC	5 cut(s)	133, 157, 619, 1222, 1597
DdeI	CTNAG	6 cut(s)	146, 405, 870, 975, 1491, 1866
DpnI	GATC	8 cut(s)	6, 113, 465, 545, 888, 1472, 1847, 1970
DpnII	GATC	8 cut(s)	4, 111, 463, 543, 886, 1470, 1845, 1968
EaeI	YGGCCR	2 cut(s)	1503, 1878
Eam1104I	CTCTTC	6 cut(s)	28, 201, 1334, 1565, 1709, 1952
EarI	CTCTTC	6 cut(s)	28, 201, 1334, 1565, 1709, 1952
EciI	GGCGGA	3 cut(s)	621, 1310, 1685
Eco147I	AGGCCT	2 cut(s)	1201, 1576
Eco24I	GRGCYC	1 cut(s)	40
Eco31I	GGTCTC	2 cut(s)	1475, 1850
Eco47I	GGWCC	2 cut(s)	256, 678
Eco57I	CTGAAG	3 cut(s)	164, 1484, 1859
Eco81I	CCTNAGG	1 cut(s)	870
EcoNI	CCTNNNNNAGG	2 cut(s)	915, 1242
EcoRII	CCWGG	5 cut(s)	301, 368, 612, 733, 800
EcoT22I	ATGCAT	1 cut(s)	1336
EcoT38I	GRGCYC	1 cut(s)	40
Esp3I	CGTCTC	1 cut(s)	1139
FaeI	CATG	8 cut(s)	124, 253, 470, 556, 685, 887, 1012, 1219
FalI	AAGNNNNNCTT	2 cut(s)	1949, 1981
FaqI	GGGAC	1 cut(s)	1596
FatI	CATG	8 cut(s)	120, 249, 466, 552, 681, 883, 1008, 1215
FauNDI	CATATG	1 cut(s)	1924
FbaI	TGATCA	4 cut(s)	463, 886, 1470, 1845
FokI	GGATG	7 cut(s)	924, 1277, 1343, 1388, 1652, 1763, 1953
FriOI	GRGCYC	1 cut(s)	40
FspBI	CTAG	2 cut(s)	498, 1538
GsuI	CTGGAG	4 cut(s)	596, 1163, 1672, 1717
HaeIII	GGCC	4 cut(s)	1201, 1505, 1576, 1880
HapII	CCGG	1 cut(s)	44
Hin1I	GRCGYC	1 cut(s)	1148
Hin1II	CATG	8 cut(s)	124, 253, 470, 556, 685, 887, 1012, 1219
HindIII	AAGCTT	2 cut(s)	653, 1322
HpaII	CCGG	1 cut(s)	44
HphI	GGTGA	5 cut(s)	95, 291, 723, 1161, 1536
HpyAV	CCTTC	7 cut(s)	50, 118, 304, 550, 736, 1148, 1361
HpyCH4III	ACNGT	8 cut(s)	271, 379, 639, 703, 811, 1002, 1020, 1587
HpyCH4IV	ACGT	2 cut(s)	1071, 1148
HpyF10VI	GCNNNNNNNGC	5 cut(s)	661, 1042, 1051, 1265, 1640
HpyF3I	CTNAG	6 cut(s)	146, 405, 870, 975, 1491, 1866
HpySE526I	ACGT	2 cut(s)	1071, 1148
Hsp92I	GRCGYC	1 cut(s)	1148
Hsp92II	CATG	8 cut(s)	124, 253, 470, 556, 685, 887, 1012, 1219
Kpn2I	TCCGGA	1 cut(s)	43
Ksp22I	TGATCA	4 cut(s)	463, 886, 1470, 1845
Kzo9I	GATC	8 cut(s)	4, 111, 463, 543, 886, 1470, 1845, 1968
LguI	GCTCTTC	2 cut(s)	28, 1952
LmnI	GCTCC	6 cut(s)	445, 688, 877, 1108, 1281, 1656
MabI	ACCWGGT	2 cut(s)	301, 733
MaeI	CTAG	2 cut(s)	498, 1538
MaeII	ACGT	2 cut(s)	1071, 1148
MaeIII	GTNAC	2 cut(s)	290, 1067
MalI	GATC	8 cut(s)	6, 113, 465, 545, 888, 1472, 1847, 1970
MboI	GATC	8 cut(s)	4, 111, 463, 543, 886, 1470, 1845, 1968
MfeI	CAATTG	1 cut(s)	1661
MflI	RGATCY	1 cut(s)	543
MhlI	GDGCHC	2 cut(s)	40, 693
MlsI	TGGCCA	1 cut(s)	1880
MluNI	TGGCCA	1 cut(s)	1880
MlyI	GAGTC	4 cut(s)	200, 632, 1243, 1618
MmeI	TCCRAC	3 cut(s)	666, 1545, 1920
Mox20I	TGGCCA	1 cut(s)	1880
Mph1103I	ATGCAT	1 cut(s)	1336
MroI	TCCGGA	1 cut(s)	43
MroXI	GAANNNNTTC	2 cut(s)	239, 588
MscI	TGGCCA	1 cut(s)	1880
MseI	TTAA	6 cut(s)	354, 480, 578, 786, 905, 1022
MslI	CAYNNNNRTG	1 cut(s)	119
Msp20I	TGGCCA	1 cut(s)	1880
MspI	CCGG	1 cut(s)	44
MspR9I	CCNGG	5 cut(s)	303, 370, 614, 735, 802
MunI	CAATTG	1 cut(s)	1661
MvaI	CCWGG	5 cut(s)	303, 370, 614, 735, 802
MvnI	CGCG	2 cut(s)	1303, 1678
MwoI	GCNNNNNNNGC	5 cut(s)	661, 1042, 1051, 1265, 1640
NdeI	CATATG	1 cut(s)	1924
NdeII	GATC	8 cut(s)	4, 111, 463, 543, 886, 1470, 1845, 1968
NlaIII	CATG	8 cut(s)	124, 253, 470, 556, 685, 887, 1012, 1219
NlaIV	GGNNCC	2 cut(s)	1283, 1658
NmuCI	GTSAC	1 cut(s)	1067
NsiI	ATGCAT	1 cut(s)	1336
NspI	RCATGY	1 cut(s)	1012
NspV	TTCGAA	1 cut(s)	1955
PaeI	GCATGC	1 cut(s)	1012
PagI	TCATGA	3 cut(s)	120, 552, 1215
PaqCI	CACCTGC	4 cut(s)	432, 864, 1180, 1555
PceI	AGGCCT	2 cut(s)	1201, 1576
PciSI	GCTCTTC	2 cut(s)	28, 1952
PdmI	GAANNNNTTC	2 cut(s)	239, 588
PfeI	GAWTC	8 cut(s)	218, 455, 967, 1166, 1298, 1508, 1541, 1673
PflFI	GACNNNGTC	2 cut(s)	1486, 1861
PfoI	TCCNGGA	1 cut(s)	612
PleI	GAGTC	4 cut(s)	199, 631, 1242, 1617
PpsI	GAGTC	4 cut(s)	199, 631, 1242, 1617
Ppu21I	YACGTR	1 cut(s)	1072
PshAI	GACNNNNGTC	1 cut(s)	925
PshBI	ATTAAT	2 cut(s)	354, 786
PsiI	TTATAA	2 cut(s)	344, 776
Psp6I	CCWGG	5 cut(s)	301, 368, 612, 733, 800
PspGI	CCWGG	5 cut(s)	301, 368, 612, 733, 800
PspN4I	GGNNCC	2 cut(s)	1283, 1658
PspPI	GGNCC	2 cut(s)	256, 678
PstI	CTGCAG	1 cut(s)	1081
PsuI	RGATCY	1 cut(s)	543
PsyI	GACNNNGTC	2 cut(s)	1486, 1861
RsaI	GTAC	5 cut(s)	134, 158, 620, 1223, 1598
RsaNI	GTAC	5 cut(s)	133, 157, 619, 1222, 1597
RseI	CAYNNNNRTG	1 cut(s)	119
SapI	GCTCTTC	2 cut(s)	28, 1952
SaqAI	TTAA	6 cut(s)	354, 480, 578, 786, 905, 1022
Sau3AI	GATC	8 cut(s)	4, 111, 463, 543, 886, 1470, 1845, 1968
Sau96I	GGNCC	2 cut(s)	256, 678
ScaI	AGTACT	1 cut(s)	134
SchI	GAGTC	4 cut(s)	200, 632, 1243, 1618
ScrFI	CCNGG	5 cut(s)	303, 370, 614, 735, 802
SduI	GDGCHC	2 cut(s)	40, 693
SexAI	ACCWGGT	2 cut(s)	301, 733
SfcI	CTRYAG	3 cut(s)	275, 1077, 1161
SfuI	TTCGAA	1 cut(s)	1955
SinI	GGWCC	2 cut(s)	256, 678
SmiMI	CAYNNNNRTG	1 cut(s)	119
SmlI	CTYRAG	6 cut(s)	656, 915, 1242, 1382, 1617, 1757
SmoI	CTYRAG	6 cut(s)	656, 915, 1242, 1382, 1617, 1757
SpeI	ACTAGT	1 cut(s)	497
SphI	GCATGC	1 cut(s)	1012
SseBI	AGGCCT	2 cut(s)	1201, 1576
SsiI	CCGC	8 cut(s)	200, 259, 632, 1295, 1303, 1359, 1670, 1678
SspI	AATATT	1 cut(s)	727
SspMI	CTAG	2 cut(s)	498, 1538
StuI	AGGCCT	2 cut(s)	1201, 1576
StyD4I	CCNGG	5 cut(s)	301, 368, 612, 733, 800
TaaI	ACNGT	8 cut(s)	271, 379, 639, 703, 811, 1002, 1020, 1587
TaiI	ACGT	2 cut(s)	1074, 1151
TaqI	TCGA	3 cut(s)	31, 1056, 1955
TatI	WGTACW	3 cut(s)	132, 1221, 1596
TauI	GCSGC	2 cut(s)	1306, 1681
TfiI	GAWTC	8 cut(s)	218, 455, 967, 1166, 1298, 1508, 1541, 1673
Tru1I	TTAA	6 cut(s)	354, 480, 578, 786, 905, 1022
Tru9I	TTAA	6 cut(s)	354, 480, 578, 786, 905, 1022
TscAI	CASTG	2 cut(s)	23, 520
TseFI	GTSAC	1 cut(s)	1067
Tsp45I	GTSAC	1 cut(s)	1067
TspDTI	ATGAA	9 cut(s)	137, 340, 569, 772, 1204, 1232, 1392, 1767, 1932
TspGWI	ACGGA	3 cut(s)	1465, 1840, 1953
TspRI	CASTG	2 cut(s)	23, 520
Tth111I	GACNNNGTC	2 cut(s)	1486, 1861
VpaK11BI	GGWCC	2 cut(s)	256, 678
VspI	ATTAAT	2 cut(s)	354, 786
XagI	CCTNNNNNAGG	2 cut(s)	915, 1242
XapI	RAATTY	6 cut(s)	58, 211, 346, 778, 1455, 1830
XbaI	TCTAGA	1 cut(s)	1537
XceI	RCATGY	1 cut(s)	1012
XmnI	GAANNNNTTC	2 cut(s)	239, 588
XspI	CTAG	2 cut(s)	498, 1538
ZraI	GACGTC	1 cut(s)	1149
ZrmI	AGTACT	1 cut(s)	134
Zsp2I	ATGCAT	1 cut(s)	1336

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.