pycom03g02650

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0001201
Physical Location & Seq
Reverse (-)
2032390 .. 2034657
2268 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g02650.6

Sequence Viewer

Length: 729 bp
ATGGATACCAATAAGTGGAGGCCTTCTCAAGTTGGAGAGGCCGCCATGGATGCCGGCGATTGGAGGAGTCAGCTGCAGGCTGATTCAAGGCAAAGAATTGTCAACAAGATAATGGATACATTGAAGAGGCATCTTCCCTTCTCCGGACAAGATGGATTGCAGGAACTCAGCAAAATTGCTGTAAGGTTTGAGGAAAAGATTTATACTGCAGCCACAAGCCAGTCGGATTATCTACGGGAAATTTCTCTGAAGATGCTCACCATGGAGACCAAGTCTCAGAATTTAATGGGCAATCATTTACAATCTAACTCTGCTGGTAACAGCAACGGGTCCCCTGATCGATCAGCTTCTCTAGATTCCTCATCTCAGACTGGATATGCAAATGGGGGTGATTGGCAAGAGGAGGTCTATCAAAAAATCAAAGTCATGAAGGAAGCGTACTTACCCGAACTGAGTGAAATGTATCAGAAAATTGCTACTAAACTTCGGCAGCACGATTCTCATCCACAACAACCAAAGTCAGAGCAGCTTGAGAAGCTAAAAATGTTCAAAACCATGTTAGAGCGCCTCATATCAATCTTACAGGTTTCCAGGAGTAACATTTCACCTGGTTTGAAAGACAAGTTGGGTTTGTATGAGAAGCAGATGGTAAATTTTATTAATACAAATAGACCGAGGAAGCAAGGTCCTCCTCTGCAACAAGGGCAGCTCCCCCCACCTCATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

243

Amino Acids

27.55

Weight (kDa)

9.35

Isoelectric Point (pI)

60.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 15
AccIII TCCGGA 1 cut(s) 143
AciI CCGC 1 cut(s) 42
AcsI RAATTY 3 cut(s) 240, 280, 652
AcuI CTGAAG 1 cut(s) 269
AfaI GTAC 1 cut(s) 440
AfiI CCNNNNNNNGG 3 cut(s) 15, 60, 143
AgsI TTSAA 4 cut(s) 87, 124, 550, 616
AjnI CCWGG 2 cut(s) 590, 607
AjuI GAANNNNNNNTTGG 2 cut(s) 608, 640
AluBI AGCT 5 cut(s) 73, 347, 529, 538, 709
AluI AGCT 5 cut(s) 73, 347, 529, 538, 709
Alw26I GTCTC 2 cut(s) 260, 279
Aor13HI TCCGGA 1 cut(s) 143
AoxI GGCC 2 cut(s) 20, 39
ApeKI GCWGC 5 cut(s) 73, 209, 490, 526, 706
ApoI RAATTY 3 cut(s) 240, 280, 652
AseI ATTAAT 1 cut(s) 660
AspLEI GCGC 1 cut(s) 567
AspS9I GGNCC 2 cut(s) 330, 686
AsuHPI GGTGA 3 cut(s) 250, 401, 597
AvaII GGWCC 2 cut(s) 330, 686
BarI GAAGNNNNNNTAC 4 cut(s) 422, 426, 454, 458
BbvI GCAGC 5 cut(s) 60, 221, 502, 538, 718
BccI CCATC 2 cut(s) 146, 640
BciT130I CCWGG 2 cut(s) 592, 609
BciVI GTATCC 1 cut(s) 109
BcoDI GTCTC 2 cut(s) 260, 279
BfaI CTAG 1 cut(s) 353
BfmI CTRYAG 2 cut(s) 74, 207
BfoI RGCGCY 1 cut(s) 568
BfuI GTATCC 1 cut(s) 109
BisI GCNGC 6 cut(s) 42, 74, 210, 491, 527, 707
BlsI GCNGC 6 cut(s) 43, 75, 211, 492, 528, 708
Bme1390I CCNGG 2 cut(s) 592, 609
Bme18I GGWCC 2 cut(s) 330, 686
BmgT120I GGNCC 2 cut(s) 330, 686
BmiI GGNNCC 2 cut(s) 331, 332
BmrFI CCNGG 2 cut(s) 592, 609
BmsI GCATC 3 cut(s) 40, 139, 243
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpuEI CTTGAG 2 cut(s) 12, 551
Bsa29I ATCGAT 1 cut(s) 340
BsaBI GATNNNNATC 1 cut(s) 501
BsaI GGTCTC 1 cut(s) 260
BsaJI CCNNGG 3 cut(s) 45, 261, 674
BsaWI WCCGGW 1 cut(s) 143
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bsc4I CCNNNNNNNGG 3 cut(s) 15, 60, 143
Bse118I RCCGGY 1 cut(s) 53
Bse1I ACTGG 2 cut(s) 220, 376
Bse8I GATNNNNATC 1 cut(s) 501
BseAI TCCGGA 1 cut(s) 143
BseBI CCWGG 2 cut(s) 592, 609
BseCI ATCGAT 1 cut(s) 340
BseDI CCNNGG 3 cut(s) 45, 261, 674
BseGI GGATG 2 cut(s) 55, 502
BseJI GATNNNNATC 1 cut(s) 501
BseLI CCNNNNNNNGG 3 cut(s) 15, 60, 143
BseMII CTCAG 4 cut(s) 181, 290, 380, 443
BseNI ACTGG 2 cut(s) 220, 376
BseRI GAGGAG 3 cut(s) 79, 416, 681
BseXI GCAGC 5 cut(s) 60, 221, 502, 538, 718
BshFI GGCC 2 cut(s) 22, 41
BshVI ATCGAT 1 cut(s) 340
BsiSI CCGG 2 cut(s) 54, 144
BslFI GGGAC 1 cut(s) 316
BslI CCNNNNNNNGG 3 cut(s) 15, 60, 143
BsmAI GTCTC 2 cut(s) 260, 279
BsmFI GGGAC 1 cut(s) 316
BsnI GGCC 2 cut(s) 22, 41
Bso31I GGTCTC 1 cut(s) 260
Bsp13I TCCGGA 1 cut(s) 143
Bsp143I GATC 2 cut(s) 337, 341
Bsp19I CCATGG 2 cut(s) 45, 261
BspACI CCGC 1 cut(s) 42
BspANI GGCC 2 cut(s) 22, 41
BspCNI CTCAG 4 cut(s) 180, 289, 379, 444
BspDI ATCGAT 1 cut(s) 340
BspEI TCCGGA 1 cut(s) 143
BspHI TCATGA 1 cut(s) 426
BspLI GGNNCC 2 cut(s) 331, 332
BspMAI CTGCAG 2 cut(s) 78, 211
BspTNI GGTCTC 1 cut(s) 260
BsrFI RCCGGY 1 cut(s) 53
BsrI ACTGG 2 cut(s) 220, 376
BssAI RCCGGY 1 cut(s) 53
BssECI CCNNGG 3 cut(s) 45, 261, 674
BssMI GATC 2 cut(s) 337, 341
BssT1I CCWWGG 2 cut(s) 45, 261
Bst2UI CCWGG 2 cut(s) 592, 609
Bst6I CTCTTC 1 cut(s) 119
BstC8I GCNNGC 2 cut(s) 55, 78
BstDEI CTNAG 4 cut(s) 167, 276, 366, 452
BstDSI CCRYGG 2 cut(s) 45, 261
BstF5I GGATG 2 cut(s) 55, 502
BstH2I RGCGCY 1 cut(s) 568
BstHHI GCGC 1 cut(s) 567
BstKTI GATC 2 cut(s) 340, 344
BstMAI GTCTC 2 cut(s) 260, 279
BstMBI GATC 2 cut(s) 337, 341
BstMWI GCNNNNNNNGC 3 cut(s) 50, 535, 703
BstNI CCWGG 2 cut(s) 592, 609
BstSCI CCNGG 2 cut(s) 590, 607
BstSFI CTRYAG 2 cut(s) 74, 207
BstV1I GCAGC 5 cut(s) 60, 221, 502, 538, 718
Bsu15I ATCGAT 1 cut(s) 340
BsuI GTATCC 1 cut(s) 109
BsuRI GGCC 2 cut(s) 22, 41
BsuTUI ATCGAT 1 cut(s) 340
BtgI CCRYGG 2 cut(s) 45, 261
BtsCI GGATG 2 cut(s) 55, 502
Cac8I GCNNGC 2 cut(s) 55, 78
CciI TCATGA 1 cut(s) 426
CfoI GCGC 1 cut(s) 567
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 2 cut(s) 330, 686
ClaI ATCGAT 1 cut(s) 340
CsiI ACCWGGT 1 cut(s) 607
Csp6I GTAC 1 cut(s) 439
CviAII CATG 4 cut(s) 46, 262, 427, 556
CviQI GTAC 1 cut(s) 439
DdeI CTNAG 4 cut(s) 167, 276, 366, 452
DpnI GATC 2 cut(s) 339, 343
DpnII GATC 2 cut(s) 337, 341
Eam1104I CTCTTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 119
Eco130I CCWWGG 2 cut(s) 45, 261
Eco147I AGGCCT 1 cut(s) 22
Eco31I GGTCTC 1 cut(s) 260
Eco47I GGWCC 2 cut(s) 330, 686
Eco57I CTGAAG 1 cut(s) 269
EcoO109I RGGNCCY 2 cut(s) 330, 686
EcoRII CCWGG 2 cut(s) 590, 607
EcoT14I CCWWGG 2 cut(s) 45, 261
ErhI CCWWGG 2 cut(s) 45, 261
FaeI CATG 4 cut(s) 49, 265, 430, 559
FaqI GGGAC 1 cut(s) 316
FatI CATG 4 cut(s) 45, 261, 426, 555
FauNDI CATATG 1 cut(s) 723
Fnu4HI GCNGC 6 cut(s) 42, 74, 210, 491, 527, 707
FokI GGATG 2 cut(s) 62, 489
Fsp4HI GCNGC 6 cut(s) 42, 74, 210, 491, 527, 707
FspBI CTAG 1 cut(s) 353
GlaI GCGC 1 cut(s) 566
GluI GCNGC 6 cut(s) 42, 74, 210, 491, 527, 707
HaeII RGCGCY 1 cut(s) 568
HaeIII GGCC 2 cut(s) 22, 41
HapII CCGG 2 cut(s) 54, 144
HhaI GCGC 1 cut(s) 567
Hin1II CATG 4 cut(s) 49, 265, 430, 559
Hin6I GCGC 1 cut(s) 565
HinP1I GCGC 1 cut(s) 565
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HinfI GANTC 4 cut(s) 67, 83, 356, 497
HpaII CCGG 2 cut(s) 54, 144
HphI GGTGA 3 cut(s) 250, 401, 597
Hpy166II GTNNAC 1 cut(s) 103
Hpy188I TCNGA 6 cut(s) 226, 249, 279, 369, 468, 523
Hpy188III TCNNGA 3 cut(s) 144, 353, 427
Hpy8I GTNNAC 1 cut(s) 103
HpyAV CCTTC 3 cut(s) 33, 148, 424
HpyCH4V TGCA 5 cut(s) 76, 160, 209, 380, 697
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 535, 703
HpyF3I CTNAG 4 cut(s) 167, 276, 366, 452
Hsp92II CATG 4 cut(s) 49, 265, 430, 559
HspAI GCGC 1 cut(s) 565
KflI GGGWCCC 1 cut(s) 330
Kpn2I TCCGGA 1 cut(s) 143
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
Kzo9I GATC 2 cut(s) 337, 341
LmnI GCTCC 1 cut(s) 714
Lsp1109I GCAGC 5 cut(s) 60, 221, 502, 538, 718
LweI GCATC 3 cut(s) 40, 139, 243
MabI ACCWGGT 1 cut(s) 607
MaeI CTAG 1 cut(s) 353
MaeIII GTNAC 2 cut(s) 317, 596
MalI GATC 2 cut(s) 339, 343
MboI GATC 2 cut(s) 337, 341
MboII GAAGA 3 cut(s) 125, 136, 262
MluCI AATT 6 cut(s) 96, 174, 240, 280, 471, 652
MlyI GAGTC 1 cut(s) 76
MmeI TCCRAC 2 cut(s) 13, 204
MroI TCCGGA 1 cut(s) 143
MroNI GCCGGC 1 cut(s) 53
MseI TTAA 2 cut(s) 284, 660
MspA1I CMGCKG 1 cut(s) 73
MspI CCGG 2 cut(s) 54, 144
MspR9I CCNGG 2 cut(s) 592, 609
MvaI CCWGG 2 cut(s) 592, 609
MwoI GCNNNNNNNGC 3 cut(s) 50, 535, 703
NaeI GCCGGC 1 cut(s) 55
NcoI CCATGG 2 cut(s) 45, 261
NdeI CATATG 1 cut(s) 723
NdeII GATC 2 cut(s) 337, 341
NgoMIV GCCGGC 1 cut(s) 53
NlaIII CATG 4 cut(s) 49, 265, 430, 559
NlaIV GGNNCC 2 cut(s) 331, 332
PagI TCATGA 1 cut(s) 426
PceI AGGCCT 1 cut(s) 22
PdiI GCCGGC 1 cut(s) 55
PfeI GAWTC 3 cut(s) 83, 356, 497
PflFI GACNNNGTC 1 cut(s) 271
PflMI CCANNNNNTGG 1 cut(s) 15
PfoI TCCNGGA 1 cut(s) 590
PkrI GCNGC 6 cut(s) 43, 75, 211, 492, 528, 708
PleI GAGTC 1 cut(s) 75
PpsI GAGTC 1 cut(s) 75
PpuMI RGGWCCY 2 cut(s) 330, 686
PshBI ATTAAT 1 cut(s) 660
Psp5II RGGWCCY 2 cut(s) 330, 686
Psp6I CCWGG 2 cut(s) 590, 607
PspGI CCWGG 2 cut(s) 590, 607
PspN4I GGNNCC 2 cut(s) 331, 332
PspPI GGNCC 2 cut(s) 330, 686
PspPPI RGGWCCY 2 cut(s) 330, 686
PstI CTGCAG 2 cut(s) 78, 211
PsyI GACNNNGTC 1 cut(s) 271
PvuII CAGCTG 1 cut(s) 73
RsaI GTAC 1 cut(s) 440
RsaNI GTAC 1 cut(s) 439
SaqAI TTAA 2 cut(s) 284, 660
SatI GCNGC 6 cut(s) 42, 74, 210, 491, 527, 707
Sau3AI GATC 2 cut(s) 337, 341
Sau96I GGNCC 2 cut(s) 330, 686
SchI GAGTC 1 cut(s) 76
ScrFI CCNGG 2 cut(s) 592, 609
SexAI ACCWGGT 1 cut(s) 607
SfaNI GCATC 3 cut(s) 40, 139, 243
SfcI CTRYAG 2 cut(s) 74, 207
SinI GGWCC 2 cut(s) 330, 686
SmlI CTYRAG 2 cut(s) 27, 530
SmoI CTYRAG 2 cut(s) 27, 530
Sse9I AATT 6 cut(s) 96, 174, 240, 280, 471, 652
SseBI AGGCCT 1 cut(s) 22
SsiI CCGC 1 cut(s) 42
SspMI CTAG 1 cut(s) 353
StuI AGGCCT 1 cut(s) 22
StyD4I CCNGG 2 cut(s) 590, 607
StyI CCWWGG 2 cut(s) 45, 261
TaqI TCGA 1 cut(s) 340
TaqII GACCGA 1 cut(s) 688
TasI AATT 6 cut(s) 96, 174, 240, 280, 471, 652
TauI GCSGC 1 cut(s) 44
TfiI GAWTC 3 cut(s) 83, 356, 497
Tru1I TTAA 2 cut(s) 284, 660
Tru9I TTAA 2 cut(s) 284, 660
TseI GCWGC 5 cut(s) 73, 209, 490, 526, 706
TspDTI ATGAA 1 cut(s) 443
Tth111I GACNNNGTC 1 cut(s) 271
Van91I CCANNNNNTGG 1 cut(s) 15
VpaK11BI GGWCC 2 cut(s) 330, 686
VspI ATTAAT 1 cut(s) 660
XapI RAATTY 3 cut(s) 240, 280, 652
XbaI TCTAGA 1 cut(s) 352
XspI CTAG 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.