MD03G1033300.v1.1

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001201
Physical Location & Seq
Reverse (-)
2673296 .. 2673906
611 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1033300.v1.1.491

Sequence Viewer

Length: 165 bp
ATGGATACCAGTAAGTGGAGGCCTTCTCAAGTTGGAGAGGCGGCCATGGATGCCGGCGATTGGAGGAGTCAGCTGCAGGCTGATTCAAGGCAAAGAATTGTCAACAATCTGCAAGTGTTTTTCTTTGTCGATTCTTTTTTTGTTTCAAGCGGTAGTCTAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

55

Amino Acids

6.13

Weight (kDa)

4.85

Isoelectric Point (pI)

47.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 15
AciI CCGC 2 cut(s) 41, 150
AcoI YGGCCR 1 cut(s) 42
AfiI CCNNNNNNNGG 2 cut(s) 15, 60
AgsI TTSAA 2 cut(s) 87, 147
AluBI AGCT 1 cut(s) 73
AluI AGCT 1 cut(s) 73
AoxI GGCC 2 cut(s) 20, 42
ApeKI GCWGC 1 cut(s) 73
BbvI GCAGC 1 cut(s) 60
BfmI CTRYAG 1 cut(s) 74
BisI GCNGC 2 cut(s) 42, 74
BlsI GCNGC 2 cut(s) 43, 75
BmsI GCATC 1 cut(s) 40
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpuEI CTTGAG 1 cut(s) 12
BsaJI CCNNGG 1 cut(s) 45
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 60
Bse118I RCCGGY 1 cut(s) 53
Bse1I ACTGG 1 cut(s) 9
BseDI CCNNGG 1 cut(s) 45
BseGI GGATG 1 cut(s) 55
BseLI CCNNNNNNNGG 2 cut(s) 15, 60
BseNI ACTGG 1 cut(s) 9
BseRI GAGGAG 1 cut(s) 79
BseXI GCAGC 1 cut(s) 60
BshFI GGCC 2 cut(s) 22, 44
BsiSI CCGG 1 cut(s) 54
BslI CCNNNNNNNGG 2 cut(s) 15, 60
BsnI GGCC 2 cut(s) 22, 44
Bsp19I CCATGG 1 cut(s) 45
BspACI CCGC 2 cut(s) 41, 150
BspANI GGCC 2 cut(s) 22, 44
BspMAI CTGCAG 1 cut(s) 78
BsrFI RCCGGY 1 cut(s) 53
BsrI ACTGG 1 cut(s) 9
BssAI RCCGGY 1 cut(s) 53
BssECI CCNNGG 1 cut(s) 45
BssT1I CCWWGG 1 cut(s) 45
BstC8I GCNNGC 2 cut(s) 55, 78
BstDSI CCRYGG 1 cut(s) 45
BstF5I GGATG 1 cut(s) 55
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstSFI CTRYAG 1 cut(s) 74
BstV1I GCAGC 1 cut(s) 60
BsuRI GGCC 2 cut(s) 22, 44
BtgI CCRYGG 1 cut(s) 45
BtsCI GGATG 1 cut(s) 55
Cac8I GCNNGC 2 cut(s) 55, 78
Cfr10I RCCGGY 1 cut(s) 53
CviAII CATG 1 cut(s) 46
CviJI RGCY 4 cut(s) 22, 44, 73, 80
CviKI_1 RGCY 4 cut(s) 22, 44, 73, 80
EaeI YGGCCR 1 cut(s) 42
Eco130I CCWWGG 1 cut(s) 45
Eco147I AGGCCT 1 cut(s) 22
EcoT14I CCWWGG 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 1 cut(s) 49
FaiI YATR 1 cut(s) 47
FatI CATG 1 cut(s) 45
Fnu4HI GCNGC 2 cut(s) 42, 74
FokI GGATG 1 cut(s) 62
Fsp4HI GCNGC 2 cut(s) 42, 74
GluI GCNGC 2 cut(s) 42, 74
HaeIII GGCC 2 cut(s) 22, 44
HapII CCGG 1 cut(s) 54
Hin1II CATG 1 cut(s) 49
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HinfI GANTC 3 cut(s) 67, 83, 131
HpaII CCGG 1 cut(s) 54
Hpy166II GTNNAC 1 cut(s) 103
Hpy8I GTNNAC 1 cut(s) 103
HpyAV CCTTC 1 cut(s) 33
HpyCH4V TGCA 2 cut(s) 76, 112
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
Hsp92II CATG 1 cut(s) 49
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
LpnPI CCDG 3 cut(s) 22, 62, 67
Lsp1109I GCAGC 1 cut(s) 60
LweI GCATC 1 cut(s) 40
MluCI AATT 2 cut(s) 96, 159
MlyI GAGTC 1 cut(s) 76
MmeI TCCRAC 1 cut(s) 13
MnlI CCTC 3 cut(s) 12, 31, 57
MroNI GCCGGC 1 cut(s) 53
MspA1I CMGCKG 1 cut(s) 73
MspI CCGG 1 cut(s) 54
MwoI GCNNNNNNNGC 1 cut(s) 50
NaeI GCCGGC 1 cut(s) 55
NcoI CCATGG 1 cut(s) 45
NgoMIV GCCGGC 1 cut(s) 53
NlaIII CATG 1 cut(s) 49
PceI AGGCCT 1 cut(s) 22
PdiI GCCGGC 1 cut(s) 55
PfeI GAWTC 2 cut(s) 83, 131
PflMI CCANNNNNTGG 1 cut(s) 15
PkrI GCNGC 2 cut(s) 43, 75
PleI GAGTC 1 cut(s) 75
PpsI GAGTC 1 cut(s) 75
PstI CTGCAG 1 cut(s) 78
PvuII CAGCTG 1 cut(s) 73
SatI GCNGC 2 cut(s) 42, 74
SchI GAGTC 1 cut(s) 76
SetI ASST 1 cut(s) 75
SfaNI GCATC 1 cut(s) 40
SfcI CTRYAG 1 cut(s) 74
SgeI CNNG 8 cut(s) 21, 41, 58, 66, 89, 99, 125, 159
SmlI CTYRAG 1 cut(s) 27
SmoI CTYRAG 1 cut(s) 27
Sse9I AATT 2 cut(s) 96, 159
SseBI AGGCCT 1 cut(s) 22
SsiI CCGC 2 cut(s) 41, 150
StuI AGGCCT 1 cut(s) 22
StyI CCWWGG 1 cut(s) 45
TaqI TCGA 1 cut(s) 129
TasI AATT 2 cut(s) 96, 159
TauI GCSGC 1 cut(s) 44
TfiI GAWTC 2 cut(s) 83, 131
TseI GCWGC 1 cut(s) 73
Van91I CCANNNNNTGG 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.