Rh5BG520700

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001201
Physical Location & Seq
Forward (+)
82327248 .. 82332669
5422 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG520700.1

Sequence Viewer

Length: 1497 bp
ATGGATCAGAAGAAGCAGTGGTATCAATCTCAAAGAGCCCTTCCCGAGAGTTCAACAAGTTCCACAACACATACAAATAGAGGTGATTGGCAAGAGGAGGTCTATCAAAAGATCATAGTCATGAAGGAGAAGTACTTGCCTGAACTAAGTGAATTGTACCAGAAAATTGATAATAAACTTCAGCAGCACGAGTCTCTTCCGCAGCCACCAAATTCAGATTATCTTGCGAAGCTCCAAATGTTCAAGTCCATGCTGGACCGCCTCATAACAGTCCTACAGGTGTCCAAAGGTAACATTTCACCTGGTTTGAAGGAGAAGTTGGGTATATACGAGAAGCAGATTATAAATTTCATTAATACAAATAGACCCAGGAAGACTGTTTCTTCTCTACATCTTCCCCCACCTGAGATGCACAATGCACACCTGCTGCAACAACCCAAGATGGAGCAACAAGAGTCAAATGATCATGTAATATATCTTAATTTCTTATCCCCGACTACTGATACAAGTGGCACTGATTGGCAAGAGGAGGTCTATCAAAAGATCCAAGTCATGAAGGAGACTTACTTGCCTGAATTAAGTGAAATGTTTCAGAAAATTGATAATAAACTCCAGGAGTACGAGTCTTTTCCGCCACAGTCAAATCCAGATAAGCTTGAGAAGCTGAAAGTGTTTAGGACCATGTTGGAGCACCTCATAACAGTCTTACAGGTTTCCAAAAGTAATATTTCACCTGGTTTGAAGGAGAAGTTGGGTTTATACGAGAAGCAGATCATAAATTTCATTAATACAAATAGACCCAGGAAGACTGTTTCTTCTCTACATCTTCCCCCACCTGATATGCACAATGCACACCTGCTGCAACAACCTAAGGTGGAGCAACAAGATCAGAGTGCATCTAACTTAATACCTACTCAAGGACAGCAGTCTCAACCTCATCCATCACAGCAACAATATCTTTCAAATATTCAATCTCAGCTTATACATATACAACAACGACAGTTGAGCATGCAACAACAGTTAATCCCACTACTGCAAGATATGCAGCAAAGGCACGAAGCATCAGGTCAGGTATCTGCAGCCATAATTCAACCACCAAATACAATGGAGCTGCAAAACCAGTTATATCAATCACATACAGCCCTTCCGGAGACATCATCAACATCTCTAGATTCGCCTGCCCTGACTGGACATACAGACAAGAGGCCTATCAAAAGGTTCATGAAGTACAACGATGGGAGTCCTCCTCAAGGTGGAGAGCCTGTAATGGATGCAGGAGATTGGAGGAGCCAATTGCAGGCGGATTCGCGGCAAAGAATTGTCAATAAGCTCATCTCTAGATTCACCTGCCCTGACTGGACATACAGACAAGAGGCCTATCGAAAGGTTCATGAAGTACAACGATGGGAGTCCCCCTCAAGGTGGAGAGCCTGCAATGGATGCAGGAGACTGGAGGAGCCAATTGCAGGCGGATTTGCGGCAAAGAATTGTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

498

Amino Acids

57.66

Weight (kDa)

8.6

Isoelectric Point (pI)

65.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 344
AarI CACCTGC 3 cut(s) 432, 864, 1355
Acc36I ACCTGC 3 cut(s) 432, 864, 1355
AccII CGCG 1 cut(s) 1309
AccIII TCCGGA 1 cut(s) 1147
AciI CCGC 7 cut(s) 200, 259, 632, 1301, 1309, 1470, 1478
AclWI GGATC 2 cut(s) 12, 538
AcsI RAATTY 3 cut(s) 211, 346, 778
AcuI CTGAAG 1 cut(s) 164
AfaI GTAC 5 cut(s) 134, 158, 620, 1229, 1398
AfiI CCNNNNNNNGG 7 cut(s) 917, 1250, 1253, 1297, 1419, 1422, 1466
AgsI TTSAA 7 cut(s) 54, 244, 310, 742, 963, 971, 1091
AjnI CCWGG 5 cut(s) 301, 368, 612, 733, 800
AjuI GAANNNNNNNTTGG 4 cut(s) 302, 334, 734, 766
AluBI AGCT 6 cut(s) 232, 655, 664, 979, 1111, 1330
AluI AGCT 6 cut(s) 232, 655, 664, 979, 1111, 1330
Alw21I GWGCWC 1 cut(s) 693
Alw26I GTCTC 5 cut(s) 198, 554, 933, 1145, 1441
AlwI GGATC 2 cut(s) 12, 538
Ama87I CYCGRG 1 cut(s) 44
Aor13HI TCCGGA 1 cut(s) 1147
AoxI GGCC 2 cut(s) 1205, 1374
ApeKI GCWGC 7 cut(s) 184, 202, 427, 859, 1045, 1079, 1111
ApoI RAATTY 3 cut(s) 211, 346, 778
AseI ATTAAT 2 cut(s) 354, 786
Asp700I GAANNNNTTC 1 cut(s) 588
AspS9I GGNCC 2 cut(s) 256, 678
AsuHPI GGTGA 4 cut(s) 95, 291, 723, 1336
AvaI CYCGRG 1 cut(s) 44
AvaII GGWCC 2 cut(s) 256, 678
AxyI CCTNAGG 1 cut(s) 870
BanII GRGCYC 1 cut(s) 40
BarI GAAGNNNNNNTAC 4 cut(s) 116, 148, 548, 580
BauI CACGAG 1 cut(s) 188
BbsI GAAGAC 2 cut(s) 380, 812
Bbv12I GWGCWC 1 cut(s) 693
BbvI GCAGC 7 cut(s) 196, 214, 414, 846, 1057, 1091, 1098
BccI CCATC 4 cut(s) 436, 949, 1229, 1398
BciT130I CCWGG 5 cut(s) 303, 370, 614, 735, 802
BclI TGATCA 1 cut(s) 463
BcoDI GTCTC 5 cut(s) 198, 554, 933, 1145, 1441
BfaI CTAG 2 cut(s) 1169, 1338
BfmI CTRYAG 2 cut(s) 275, 1077
BfuAI ACCTGC 3 cut(s) 432, 864, 1355
BisI GCNGC 9 cut(s) 185, 203, 428, 860, 1046, 1080, 1112, 1310, 1479
BlsI GCNGC 9 cut(s) 186, 204, 429, 861, 1047, 1081, 1113, 1311, 1480
BmcAI AGTACT 1 cut(s) 134
Bme1390I CCNGG 5 cut(s) 303, 370, 614, 735, 802
Bme18I GGWCC 2 cut(s) 256, 678
BmeT110I CYCGRG 1 cut(s) 44
BmgT120I GGNCC 2 cut(s) 256, 678
BmiI GGNNCC 2 cut(s) 1289, 1458
BmrFI CCNGG 5 cut(s) 303, 370, 614, 735, 802
BmsI GCATC 5 cut(s) 399, 905, 1070, 1261, 1430
BoxI GACNNNNGTC 1 cut(s) 925
BpiI GAAGAC 2 cut(s) 380, 812
BplI GAGNNNNNCTC 4 cut(s) 1231, 1263, 1400, 1432
BpmI CTGGAG 2 cut(s) 596, 1472
BpuEI CTTGAG 4 cut(s) 677, 900, 1233, 1402
BsaJI CCNNGG 2 cut(s) 368, 800
BsaWI WCCGGW 1 cut(s) 1147
BsaXI ACNNNNNCTCC 4 cut(s) 608, 638, 1248, 1278
Bsc4I CCNNNNNNNGG 7 cut(s) 917, 1250, 1253, 1297, 1419, 1422, 1466
Bse1I ACTGG 4 cut(s) 1120, 1192, 1361, 1455
Bse21I CCTNAGG 1 cut(s) 870
Bse3DI GCAATG 1 cut(s) 1441
BseAI TCCGGA 1 cut(s) 1147
BseBI CCWGG 5 cut(s) 303, 370, 614, 735, 802
BseDI CCNNGG 2 cut(s) 368, 800
BseGI GGATG 3 cut(s) 937, 1276, 1445
BseLI CCNNNNNNNGG 7 cut(s) 917, 1250, 1253, 1297, 1419, 1422, 1466
BseMI GCAATG 1 cut(s) 1441
BseMII CTCAG 2 cut(s) 396, 989
BseNI ACTGG 4 cut(s) 1120, 1192, 1361, 1455
BseRI GAGGAG 5 cut(s) 110, 542, 1236, 1300, 1469
BseXI GCAGC 7 cut(s) 196, 214, 414, 846, 1057, 1091, 1098
Bsh1236I CGCG 1 cut(s) 1309
BshFI GGCC 2 cut(s) 1207, 1376
BsiHKAI GWGCWC 1 cut(s) 693
BsiHKCI CYCGRG 1 cut(s) 44
BsiSI CCGG 1 cut(s) 1148
BslFI GGGAC 1 cut(s) 1396
BslI CCNNNNNNNGG 7 cut(s) 917, 1250, 1253, 1297, 1419, 1422, 1466
BsmAI GTCTC 5 cut(s) 198, 554, 933, 1145, 1441
BsmFI GGGAC 1 cut(s) 1396
BsnI GGCC 2 cut(s) 1207, 1376
BsoBI CYCGRG 1 cut(s) 44
Bsp1286I GDGCHC 2 cut(s) 40, 693
Bsp13I TCCGGA 1 cut(s) 1147
Bsp143I GATC 6 cut(s) 4, 111, 463, 543, 771, 886
BspACI CCGC 7 cut(s) 200, 259, 632, 1301, 1309, 1470, 1478
BspANI GGCC 2 cut(s) 1207, 1376
BspCNI CTCAG 2 cut(s) 397, 988
BspEI TCCGGA 1 cut(s) 1147
BspFNI CGCG 1 cut(s) 1309
BspHI TCATGA 4 cut(s) 120, 552, 1221, 1390
BspLI GGNNCC 2 cut(s) 1289, 1458
BspMAI CTGCAG 1 cut(s) 1081
BspMI ACCTGC 3 cut(s) 432, 864, 1355
BspPI GGATC 2 cut(s) 12, 538
BsrDI GCAATG 1 cut(s) 1441
BsrI ACTGG 4 cut(s) 1120, 1192, 1361, 1455
BssECI CCNNGG 2 cut(s) 368, 800
BssMI GATC 6 cut(s) 4, 111, 463, 543, 771, 886
BssSI CACGAG 1 cut(s) 188
Bst2BI CACGAG 1 cut(s) 188
Bst2UI CCWGG 5 cut(s) 303, 370, 614, 735, 802
Bst4CI ACNGT 7 cut(s) 271, 379, 639, 703, 811, 1002, 1020
Bst6I CTCTTC 1 cut(s) 201
BstAPI GCANNNNNTGC 2 cut(s) 1042, 1440
BstC8I GCNNGC 5 cut(s) 1010, 1179, 1299, 1432, 1468
BstDEI CTNAG 4 cut(s) 146, 405, 870, 975
BstENI CCTNNNNNAGG 2 cut(s) 915, 1248
BstF5I GGATG 3 cut(s) 937, 1276, 1445
BstFNI CGCG 1 cut(s) 1309
BstKTI GATC 6 cut(s) 7, 114, 466, 546, 774, 889
BstMAI GTCTC 5 cut(s) 198, 554, 933, 1145, 1441
BstMBI GATC 6 cut(s) 4, 111, 463, 543, 771, 886
BstMWI GCNNNNNNNGC 4 cut(s) 661, 1042, 1051, 1440
BstNI CCWGG 5 cut(s) 303, 370, 614, 735, 802
BstNSI RCATGY 1 cut(s) 1012
BstPAI GACNNNNGTC 1 cut(s) 925
BstSCI CCNGG 5 cut(s) 301, 368, 612, 733, 800
BstSFI CTRYAG 2 cut(s) 275, 1077
BstUI CGCG 1 cut(s) 1309
BstV1I GCAGC 7 cut(s) 196, 214, 414, 846, 1057, 1091, 1098
BstV2I GAAGAC 2 cut(s) 380, 812
BstX2I RGATCY 1 cut(s) 543
BstYI RGATCY 1 cut(s) 543
Bsu36I CCTNAGG 1 cut(s) 870
BsuRI GGCC 2 cut(s) 1207, 1376
BtsCI GGATG 3 cut(s) 937, 1276, 1445
BtsI GCAGTG 1 cut(s) 23
BtsIMutI CAGTG 2 cut(s) 23, 513
BveI ACCTGC 3 cut(s) 432, 864, 1355
Cac8I GCNNGC 5 cut(s) 1010, 1179, 1299, 1432, 1468
CciI TCATGA 4 cut(s) 120, 552, 1221, 1390
Cfr13I GGNCC 2 cut(s) 256, 678
CsiI ACCWGGT 2 cut(s) 301, 733
Csp6I GTAC 5 cut(s) 133, 157, 619, 1228, 1397
CviAII CATG 8 cut(s) 121, 250, 467, 553, 682, 1009, 1222, 1391
CviQI GTAC 5 cut(s) 133, 157, 619, 1228, 1397
DdeI CTNAG 4 cut(s) 146, 405, 870, 975
DpnI GATC 6 cut(s) 6, 113, 465, 545, 773, 888
DpnII GATC 6 cut(s) 4, 111, 463, 543, 771, 886
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
EciI GGCGGA 3 cut(s) 621, 1316, 1485
Eco147I AGGCCT 2 cut(s) 1207, 1376
Eco24I GRGCYC 1 cut(s) 40
Eco47I GGWCC 2 cut(s) 256, 678
Eco57I CTGAAG 1 cut(s) 164
Eco81I CCTNAGG 1 cut(s) 870
Eco88I CYCGRG 1 cut(s) 44
EcoNI CCTNNNNNAGG 2 cut(s) 915, 1248
EcoRII CCWGG 5 cut(s) 301, 368, 612, 733, 800
EcoT38I GRGCYC 1 cut(s) 40
FaeI CATG 8 cut(s) 124, 253, 470, 556, 685, 1012, 1225, 1394
FaqI GGGAC 1 cut(s) 1396
FatI CATG 8 cut(s) 120, 249, 466, 552, 681, 1008, 1221, 1390
FbaI TGATCA 1 cut(s) 463
Fnu4HI GCNGC 9 cut(s) 185, 203, 428, 860, 1046, 1080, 1112, 1310, 1479
FokI GGATG 3 cut(s) 924, 1283, 1452
FriOI GRGCYC 1 cut(s) 40
Fsp4HI GCNGC 9 cut(s) 185, 203, 428, 860, 1046, 1080, 1112, 1310, 1479
FspBI CTAG 2 cut(s) 1169, 1338
GluI GCNGC 9 cut(s) 185, 203, 428, 860, 1046, 1080, 1112, 1310, 1479
GsuI CTGGAG 2 cut(s) 596, 1472
HaeIII GGCC 2 cut(s) 1207, 1376
HapII CCGG 1 cut(s) 1148
Hin1II CATG 8 cut(s) 124, 253, 470, 556, 685, 1012, 1225, 1394
HindIII AAGCTT 1 cut(s) 653
HinfI GANTC 8 cut(s) 191, 455, 623, 1172, 1240, 1304, 1341, 1409
HpaII CCGG 1 cut(s) 1148
HphI GGTGA 4 cut(s) 95, 291, 723, 1336
Hpy188I TCNGA 4 cut(s) 9, 217, 594, 891
Hpy188III TCNNGA 9 cut(s) 44, 121, 553, 647, 1148, 1169, 1222, 1338, 1391
HpyAV CCTTC 6 cut(s) 50, 118, 304, 550, 736, 1154
HpyCH4III ACNGT 7 cut(s) 271, 379, 639, 703, 811, 1002, 1020
HpyF10VI GCNNNNNNNGC 4 cut(s) 661, 1042, 1051, 1440
HpyF3I CTNAG 4 cut(s) 146, 405, 870, 975
Hsp92II CATG 8 cut(s) 124, 253, 470, 556, 685, 1012, 1225, 1394
Kpn2I TCCGGA 1 cut(s) 1147
Ksp22I TGATCA 1 cut(s) 463
Kzo9I GATC 6 cut(s) 4, 111, 463, 543, 771, 886
LmnI GCTCC 7 cut(s) 237, 445, 688, 877, 1108, 1287, 1456
Lsp1109I GCAGC 7 cut(s) 196, 214, 414, 846, 1057, 1091, 1098
LweI GCATC 5 cut(s) 399, 905, 1070, 1261, 1430
MabI ACCWGGT 2 cut(s) 301, 733
MaeI CTAG 2 cut(s) 1169, 1338
MaeIII GTNAC 1 cut(s) 290
MalI GATC 6 cut(s) 6, 113, 465, 545, 773, 888
MboI GATC 6 cut(s) 4, 111, 463, 543, 771, 886
MboII GAAGA 8 cut(s) 22, 188, 375, 385, 386, 807, 817, 818
MfeI CAATTG 2 cut(s) 1292, 1461
MflI RGATCY 1 cut(s) 543
MhlI GDGCHC 2 cut(s) 40, 693
MlyI GAGTC 5 cut(s) 200, 464, 632, 1249, 1418
MmeI TCCRAC 1 cut(s) 666
MroI TCCGGA 1 cut(s) 1147
MroXI GAANNNNTTC 1 cut(s) 588
MseI TTAA 6 cut(s) 354, 480, 578, 786, 905, 1022
MslI CAYNNNNRTG 1 cut(s) 119
MspI CCGG 1 cut(s) 1148
MspR9I CCNGG 5 cut(s) 303, 370, 614, 735, 802
MunI CAATTG 2 cut(s) 1292, 1461
MvaI CCWGG 5 cut(s) 303, 370, 614, 735, 802
MvnI CGCG 1 cut(s) 1309
MwoI GCNNNNNNNGC 4 cut(s) 661, 1042, 1051, 1440
NdeII GATC 6 cut(s) 4, 111, 463, 543, 771, 886
NlaIII CATG 8 cut(s) 124, 253, 470, 556, 685, 1012, 1225, 1394
NlaIV GGNNCC 2 cut(s) 1289, 1458
NspI RCATGY 1 cut(s) 1012
PaeI GCATGC 1 cut(s) 1012
PagI TCATGA 4 cut(s) 120, 552, 1221, 1390
PaqCI CACCTGC 3 cut(s) 432, 864, 1355
PceI AGGCCT 2 cut(s) 1207, 1376
PdmI GAANNNNTTC 1 cut(s) 588
PfeI GAWTC 3 cut(s) 1172, 1304, 1341
PfoI TCCNGGA 1 cut(s) 612
PkrI GCNGC 9 cut(s) 186, 204, 429, 861, 1047, 1081, 1113, 1311, 1480
PleI GAGTC 5 cut(s) 199, 463, 631, 1248, 1417
PpsI GAGTC 5 cut(s) 199, 463, 631, 1248, 1417
PshAI GACNNNNGTC 1 cut(s) 925
PshBI ATTAAT 2 cut(s) 354, 786
PsiI TTATAA 1 cut(s) 344
Psp6I CCWGG 5 cut(s) 301, 368, 612, 733, 800
PspGI CCWGG 5 cut(s) 301, 368, 612, 733, 800
PspN4I GGNNCC 2 cut(s) 1289, 1458
PspPI GGNCC 2 cut(s) 256, 678
PstI CTGCAG 1 cut(s) 1081
PsuI RGATCY 1 cut(s) 543
RsaI GTAC 5 cut(s) 134, 158, 620, 1229, 1398
RsaNI GTAC 5 cut(s) 133, 157, 619, 1228, 1397
RseI CAYNNNNRTG 1 cut(s) 119
SaqAI TTAA 6 cut(s) 354, 480, 578, 786, 905, 1022
SatI GCNGC 9 cut(s) 185, 203, 428, 860, 1046, 1080, 1112, 1310, 1479
Sau3AI GATC 6 cut(s) 4, 111, 463, 543, 771, 886
Sau96I GGNCC 2 cut(s) 256, 678
ScaI AGTACT 1 cut(s) 134
SchI GAGTC 5 cut(s) 200, 464, 632, 1249, 1418
ScrFI CCNGG 5 cut(s) 303, 370, 614, 735, 802
SduI GDGCHC 2 cut(s) 40, 693
SexAI ACCWGGT 2 cut(s) 301, 733
SfaNI GCATC 5 cut(s) 399, 905, 1070, 1261, 1430
SfcI CTRYAG 2 cut(s) 275, 1077
SinI GGWCC 2 cut(s) 256, 678
SmiMI CAYNNNNRTG 1 cut(s) 119
SmlI CTYRAG 4 cut(s) 656, 915, 1248, 1417
SmoI CTYRAG 4 cut(s) 656, 915, 1248, 1417
SphI GCATGC 1 cut(s) 1012
SseBI AGGCCT 2 cut(s) 1207, 1376
SsiI CCGC 7 cut(s) 200, 259, 632, 1301, 1309, 1470, 1478
SspI AATATT 2 cut(s) 727, 967
SspMI CTAG 2 cut(s) 1169, 1338
StuI AGGCCT 2 cut(s) 1207, 1376
StyD4I CCNGG 5 cut(s) 301, 368, 612, 733, 800
TaaI ACNGT 7 cut(s) 271, 379, 639, 703, 811, 1002, 1020
TaqI TCGA 1 cut(s) 1381
TatI WGTACW 3 cut(s) 132, 1227, 1396
TauI GCSGC 2 cut(s) 1312, 1481
TfiI GAWTC 3 cut(s) 1172, 1304, 1341
Tru1I TTAA 6 cut(s) 354, 480, 578, 786, 905, 1022
Tru9I TTAA 6 cut(s) 354, 480, 578, 786, 905, 1022
TscAI CASTG 2 cut(s) 23, 520
TseI GCWGC 7 cut(s) 184, 202, 427, 859, 1045, 1079, 1111
TspDTI ATGAA 8 cut(s) 137, 340, 569, 772, 1210, 1238, 1379, 1407
TspRI CASTG 2 cut(s) 23, 520
VpaK11BI GGWCC 2 cut(s) 256, 678
VspI ATTAAT 2 cut(s) 354, 786
XagI CCTNNNNNAGG 2 cut(s) 915, 1248
XapI RAATTY 3 cut(s) 211, 346, 778
XbaI TCTAGA 2 cut(s) 1168, 1337
XceI RCATGY 1 cut(s) 1012
XmnI GAANNNNTTC 1 cut(s) 588
XspI CTAG 2 cut(s) 1169, 1338
ZrmI AGTACT 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.