MD02G1272400.v1.1

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001201
Physical Location & Seq
Forward (+)
32687526 .. 32692484
4959 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1272400.v1.1.491

Sequence Viewer

Length: 1464 bp
ATGGATTCAGATAACGGGACGCCGCGGCCTCGGCCTCCTCAAGGTGATGGATTCAGAGCAGGCCAGGGCGATTGGAGGAGTCAATCTCTGCCGTGTTCACGACAAGAAATTGCCATCAAGATATTCGATACATTGAAGACGCATCGTTCCTCATCTGGCCAAGAGGAATTACCAGAACTCATGAAAATTGCCGCAGTCTTTGAGGAAAATGTTTTTACTTCTGCCACAAGCCAGTGGGATTATCTACAGAAGATTTCTACAAAGATGTTTACTGTGGAGGCCAAGTCTCCGAACACAATATTGGACACTTCTTTACAAGCTAACTCTTCTGGATCATTATTGATACATTTGGTTCGCATTTTTCTTGACCAATGTTCTAAAGATTCCCCAGCTCCGACAAGACATGCAAATGAGGCTATTTGGCAGGAGGAGGTCTATCAAAAGATCAAAGACATGAAGGAGATGTACTTACCCGAACTGAGTGAAATGTATCAGAAGATTACTAATAAAATTCAGCAGCACGAATATTGTCTTTCAACACAACCAAAATCAGAGGAGCTTGAGAAGCTAAAAATGTTCAAGACAATGTTGGAGCGTGTTATATTAGTCTTGCAGGTTTCCAAAAGTAGCATTTCACCTAGTTTGAAGGACAAGTTGTGCTTATATGAGAAGCAGATTATAAAGTTTATTAGTACAAACAGACCAAGGAAGCCAGTTTTATCTCTGCAGCAAGGGCAGCTCTCCTCATCTCAAATACACAACATGCAACCGTCACAATTTCACACTACTCAAGTCCATCTTGAAAATCAAATGAACCCATTGCTGCAGAACAACATGACTAGGTTGCAGCAAAATTCTGTGTCTTCTGTAGCTGGGGTTTCAATGGCACAGAAGAACATGTTGACCGTGGGCATAAAACGAGACAGGGAAGAAAGTCTTGAAGCATCACGTCAGGTATCAAGAACCACACTTCAACAACAAAATTCAACGGATCAACAAAAGCAGCTGCATCAATCACAAAGAGCCCTTCCTGAGACGCCATCAAATTGCAGGGTCCAGACTGGGAATGCAACTGTGGGTGATTGGCAAGAGGAGGTCTTTCAGAAAATCAAAGTCATGAAGGAAATGTACTTGCCCGAGCTAACTGAAATTCATCAGAAAATTATTACTAAACTTCCACAGCTCGTTTCTCTTCCAGAATATTCAGAGCGGAGTGAGAAGCAAAAAATGTTCAAGACCATGATCGAGCGCCTCATATCAGCCCTACAGAGTTCCAAAAATTACATTACACCTAGTTTGAAAGACAAATGGGGCTTATACGAGAAGCAGATACTAAACTTCATTGATGCAAATAGACCTAGGAAGCAGATCCCCTCGTCTGATTTGCACTCCACCCAGCAGCCATACTCTCAACACTGTGCCGCAGAAGTAAAACTAGAGCCAATAGAAGGCGAGGGTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

488

Amino Acids

55.89

Weight (kDa)

8.77

Isoelectric Point (pI)

56.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 22 - 98 1.8e-21 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 680
Acc36I ACCTGC 1 cut(s) 604
AccBSI CCGCTC 1 cut(s) 1210
AccII CGCG 1 cut(s) 25
AciI CCGC 5 cut(s) 23, 25, 192, 1210, 1422
AclWI GGATC 3 cut(s) 340, 999, 1363
AcoI YGGCCR 1 cut(s) 157
AcsI RAATTY 4 cut(s) 510, 853, 982, 1149
AcyI GRCGYC 2 cut(s) 20, 1037
AfaI GTAC 3 cut(s) 467, 694, 1130
AfiI CCNNNNNNNGG 2 cut(s) 41, 1448
AflIII ACRYGT 1 cut(s) 897
AjiI CACGTC 1 cut(s) 950
AjnI CCWGG 1 cut(s) 63
AjuI GAANNNNNNNTTGG 2 cut(s) 284, 316
AluBI AGCT 9 cut(s) 320, 392, 559, 568, 739, 872, 1006, 1141, 1183
AluI AGCT 9 cut(s) 320, 392, 559, 568, 739, 872, 1006, 1141, 1183
Alw26I GTCTC 3 cut(s) 291, 915, 1028
AlwI GGATC 3 cut(s) 340, 999, 1363
Ama87I CYCGRG 1 cut(s) 1136
AoxI GGCC 5 cut(s) 26, 32, 61, 157, 279
ApeKI GCWGC 8 cut(s) 517, 727, 736, 823, 847, 1003, 1006, 1399
ApoI RAATTY 4 cut(s) 510, 853, 982, 1149
AspA2I CCTAGG 1 cut(s) 1358
AspLEI GCGC 1 cut(s) 1251
AspS9I GGNCC 1 cut(s) 1054
AsuHPI GGTGA 3 cut(s) 56, 627, 1091
AvaI CYCGRG 1 cut(s) 1136
AvaII GGWCC 1 cut(s) 1054
AvrII CCTAGG 1 cut(s) 1358
BalI TGGCCA 1 cut(s) 159
BanII GRGCYC 1 cut(s) 1027
BarI GAAGNNNNNNTAC 4 cut(s) 449, 481, 1112, 1144
BbsI GAAGAC 2 cut(s) 143, 855
BbvI GCAGC 8 cut(s) 529, 739, 748, 810, 859, 993, 1015, 1411
BccI CCATC 4 cut(s) 41, 122, 804, 1048
BceAI ACGGC 1 cut(s) 76
BciT130I CCWGG 1 cut(s) 65
BcoDI GTCTC 3 cut(s) 291, 915, 1028
BfaI CTAG 5 cut(s) 639, 840, 1293, 1359, 1436
BfmI CTRYAG 5 cut(s) 245, 725, 824, 867, 1265
BfoI RGCGCY 1 cut(s) 1252
BfuAI ACCTGC 1 cut(s) 604
BlnI CCTAGG 1 cut(s) 1358
Bme1390I CCNGG 1 cut(s) 65
Bme18I GGWCC 1 cut(s) 1054
BmeT110I CYCGRG 1 cut(s) 1136
BmgBI CACGTC 1 cut(s) 950
BmgT120I GGNCC 1 cut(s) 1054
BmiI GGNNCC 1 cut(s) 1055
BmrFI CCNGG 1 cut(s) 65
BmrI ACTGGG 1 cut(s) 1071
BmsI GCATC 4 cut(s) 151, 953, 1018, 1336
BmuI ACTGGG 1 cut(s) 1071
BpiI GAAGAC 2 cut(s) 143, 855
BplI GAGNNNNNCTC 2 cut(s) 70, 102
BpuEI CTTGAG 3 cut(s) 24, 581, 774
BsaHI GRCGYC 2 cut(s) 20, 1037
BsaJI CCNNGG 6 cut(s) 23, 29, 64, 704, 906, 1358
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 2 cut(s) 41, 1448
Bse1I ACTGG 3 cut(s) 232, 713, 1066
Bse3DI GCAATG 1 cut(s) 818
BseBI CCWGG 1 cut(s) 65
BseDI CCNNGG 6 cut(s) 23, 29, 64, 704, 906, 1358
BseLI CCNNNNNNNGG 2 cut(s) 41, 1448
BseMI GCAATG 1 cut(s) 818
BseMII CTCAG 2 cut(s) 470, 1023
BseNI ACTGG 3 cut(s) 232, 713, 1066
BseRI GAGGAG 6 cut(s) 27, 91, 443, 569, 733, 1106
BseXI GCAGC 8 cut(s) 529, 739, 748, 810, 859, 993, 1015, 1411
BseYI CCCAGC 3 cut(s) 388, 872, 1395
Bsh1236I CGCG 1 cut(s) 25
BshFI GGCC 5 cut(s) 28, 34, 63, 159, 281
BsiHKCI CYCGRG 1 cut(s) 1136
BslFI GGGAC 1 cut(s) 31
BslI CCNNNNNNNGG 2 cut(s) 41, 1448
BsmAI GTCTC 3 cut(s) 291, 915, 1028
BsmBI CGTCTC 1 cut(s) 1028
BsmFI GGGAC 1 cut(s) 31
BsmI GAATGC 1 cut(s) 1072
BsnI GGCC 5 cut(s) 28, 34, 63, 159, 281
BsoBI CYCGRG 1 cut(s) 1136
Bsp1286I GDGCHC 1 cut(s) 1027
Bsp143I GATC 5 cut(s) 332, 444, 991, 1242, 1368
BspACI CCGC 5 cut(s) 23, 25, 192, 1210, 1422
BspANI GGCC 5 cut(s) 28, 34, 63, 159, 281
BspCNI CTCAG 2 cut(s) 471, 1024
BspFNI CGCG 1 cut(s) 25
BspHI TCATGA 2 cut(s) 180, 1116
BspLI GGNNCC 1 cut(s) 1055
BspMAI CTGCAG 2 cut(s) 729, 828
BspMI ACCTGC 1 cut(s) 604
BspPI GGATC 3 cut(s) 340, 999, 1363
BsrBI CCGCTC 1 cut(s) 1210
BsrDI GCAATG 1 cut(s) 818
BsrI ACTGG 3 cut(s) 232, 713, 1066
BssECI CCNNGG 6 cut(s) 23, 29, 64, 704, 906, 1358
BssMI GATC 5 cut(s) 332, 444, 991, 1242, 1368
BssNI GRCGYC 2 cut(s) 20, 1037
BssT1I CCWWGG 2 cut(s) 704, 1358
Bst2UI CCWGG 1 cut(s) 65
Bst4CI ACNGT 5 cut(s) 274, 771, 907, 1075, 1418
Bst6I CTCTTC 2 cut(s) 331, 1197
BstACI GRCGYC 2 cut(s) 20, 1037
BstC8I GCNNGC 1 cut(s) 61
BstDEI CTNAG 2 cut(s) 479, 1032
BstDSI CCRYGG 2 cut(s) 23, 906
BstENI CCTNNNNNAGG 1 cut(s) 39
BstFNI CGCG 1 cut(s) 25
BstH2I RGCGCY 1 cut(s) 1252
BstHHI GCGC 1 cut(s) 1251
BstKTI GATC 5 cut(s) 335, 447, 994, 1245, 1371
BstMAI GTCTC 3 cut(s) 291, 915, 1028
BstMBI GATC 5 cut(s) 332, 444, 991, 1242, 1368
BstMWI GCNNNNNNNGC 5 cut(s) 31, 413, 565, 733, 736
BstNI CCWGG 1 cut(s) 65
BstNSI RCATGY 3 cut(s) 407, 766, 901
BstSCI CCNGG 1 cut(s) 63
BstSFI CTRYAG 5 cut(s) 245, 725, 824, 867, 1265
BstUI CGCG 1 cut(s) 25
BstV1I GCAGC 8 cut(s) 529, 739, 748, 810, 859, 993, 1015, 1411
BstV2I GAAGAC 2 cut(s) 143, 855
BstX2I RGATCY 1 cut(s) 1368
BstYI RGATCY 1 cut(s) 1368
BsuRI GGCC 5 cut(s) 28, 34, 63, 159, 281
BtgI CCRYGG 2 cut(s) 23, 906
BtrI CACGTC 1 cut(s) 950
BtsIMutI CAGTG 2 cut(s) 239, 1414
BveI ACCTGC 1 cut(s) 604
Cac8I GCNNGC 1 cut(s) 61
CciI TCATGA 2 cut(s) 180, 1116
CfoI GCGC 1 cut(s) 1251
Cfr13I GGNCC 1 cut(s) 1054
Cfr42I CCGCGG 1 cut(s) 26
CseI GACGC 3 cut(s) 28, 148, 1045
Csp6I GTAC 3 cut(s) 466, 693, 1129
CviAII CATG 8 cut(s) 181, 404, 454, 763, 835, 898, 1117, 1240
CviQI GTAC 3 cut(s) 466, 693, 1129
DdeI CTNAG 2 cut(s) 479, 1032
DpnI GATC 5 cut(s) 334, 446, 993, 1244, 1370
DpnII GATC 5 cut(s) 332, 444, 991, 1242, 1368
EaeI YGGCCR 1 cut(s) 157
Eam1104I CTCTTC 2 cut(s) 331, 1197
EarI CTCTTC 2 cut(s) 331, 1197
Eco130I CCWWGG 2 cut(s) 704, 1358
Eco24I GRGCYC 1 cut(s) 1027
Eco47I GGWCC 1 cut(s) 1054
Eco88I CYCGRG 1 cut(s) 1136
EcoNI CCTNNNNNAGG 1 cut(s) 39
EcoRII CCWGG 1 cut(s) 63
EcoT14I CCWWGG 2 cut(s) 704, 1358
EcoT38I GRGCYC 1 cut(s) 1027
ErhI CCWWGG 2 cut(s) 704, 1358
Esp3I CGTCTC 1 cut(s) 1028
FaeI CATG 8 cut(s) 184, 407, 457, 766, 838, 901, 1120, 1243
FalI AAGNNNNNCTT 6 cut(s) 644, 676, 783, 815, 921, 953
FaqI GGGAC 1 cut(s) 31
FatI CATG 8 cut(s) 180, 403, 453, 762, 834, 897, 1116, 1239
FriOI GRGCYC 1 cut(s) 1027
FspBI CTAG 5 cut(s) 639, 840, 1293, 1359, 1436
GlaI GCGC 1 cut(s) 1250
GsaI CCCAGC 3 cut(s) 392, 876, 1399
HaeII RGCGCY 1 cut(s) 1252
HaeIII GGCC 5 cut(s) 28, 34, 63, 159, 281
HgaI GACGC 3 cut(s) 28, 148, 1045
HhaI GCGC 1 cut(s) 1251
Hin1I GRCGYC 2 cut(s) 20, 1037
Hin1II CATG 8 cut(s) 184, 407, 457, 766, 838, 901, 1120, 1243
Hin6I GCGC 1 cut(s) 1249
HinP1I GCGC 1 cut(s) 1249
HincII GTYRAC 1 cut(s) 903
HindII GTYRAC 1 cut(s) 903
HinfI GANTC 4 cut(s) 5, 51, 79, 383
HphI GGTGA 3 cut(s) 56, 627, 1091
Hpy166II GTNNAC 3 cut(s) 98, 270, 903
Hpy8I GTNNAC 3 cut(s) 98, 270, 903
HpyAV CCTTC 5 cut(s) 451, 640, 1037, 1114, 1442
HpyCH4III ACNGT 5 cut(s) 274, 771, 907, 1075, 1418
HpyCH4IV ACGT 1 cut(s) 949
HpyF10VI GCNNNNNNNGC 5 cut(s) 31, 413, 565, 733, 736
HpyF3I CTNAG 2 cut(s) 479, 1032
HpySE526I ACGT 1 cut(s) 949
Hsp92I GRCGYC 2 cut(s) 20, 1037
Hsp92II CATG 8 cut(s) 184, 407, 457, 766, 838, 901, 1120, 1243
HspAI GCGC 1 cut(s) 1249
KspI CCGCGG 1 cut(s) 26
Kzo9I GATC 5 cut(s) 332, 444, 991, 1242, 1368
LmnI GCTCC 3 cut(s) 397, 556, 592
Lsp1109I GCAGC 8 cut(s) 529, 739, 748, 810, 859, 993, 1015, 1411
LweI GCATC 4 cut(s) 151, 953, 1018, 1336
MaeI CTAG 5 cut(s) 639, 840, 1293, 1359, 1436
MaeII ACGT 1 cut(s) 949
MaeIII GTNAC 2 cut(s) 771, 1457
MalI GATC 5 cut(s) 334, 446, 993, 1244, 1370
MbiI CCGCTC 1 cut(s) 1210
MboI GATC 5 cut(s) 332, 444, 991, 1242, 1368
MboII GAAGA 8 cut(s) 148, 262, 318, 508, 855, 904, 941, 1184
MflI RGATCY 1 cut(s) 1368
MhlI GDGCHC 1 cut(s) 1027
MlsI TGGCCA 1 cut(s) 159
MluNI TGGCCA 1 cut(s) 159
MlyI GAGTC 1 cut(s) 88
MmeI TCCRAC 2 cut(s) 419, 570
Mox20I TGGCCA 1 cut(s) 159
MscI TGGCCA 1 cut(s) 159
MslI CAYNNNNRTG 1 cut(s) 408
Msp20I TGGCCA 1 cut(s) 159
MspA1I CMGCKG 2 cut(s) 25, 1006
MspR9I CCNGG 1 cut(s) 65
Mva1269I GAATGC 1 cut(s) 1072
MvaI CCWGG 1 cut(s) 65
MvnI CGCG 1 cut(s) 25
MwoI GCNNNNNNNGC 5 cut(s) 31, 413, 565, 733, 736
NdeII GATC 5 cut(s) 332, 444, 991, 1242, 1368
NlaIII CATG 8 cut(s) 184, 407, 457, 766, 838, 901, 1120, 1243
NlaIV GGNNCC 1 cut(s) 1055
NmeAIII GCCGAG 1 cut(s) 10
NmuCI GTSAC 1 cut(s) 771
NspI RCATGY 3 cut(s) 407, 766, 901
PagI TCATGA 2 cut(s) 180, 1116
PciI ACATGT 1 cut(s) 897
PctI GAATGC 1 cut(s) 1072
PfeI GAWTC 3 cut(s) 5, 51, 383
PleI GAGTC 1 cut(s) 87
PpsI GAGTC 1 cut(s) 87
PscI ACATGT 1 cut(s) 897
PsiI TTATAA 1 cut(s) 680
Psp6I CCWGG 1 cut(s) 63
PspFI CCCAGC 3 cut(s) 388, 872, 1395
PspGI CCWGG 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 1055
PspPI GGNCC 1 cut(s) 1054
PstI CTGCAG 2 cut(s) 729, 828
PsuI RGATCY 1 cut(s) 1368
PvuII CAGCTG 1 cut(s) 1006
RsaI GTAC 3 cut(s) 467, 694, 1130
RsaNI GTAC 3 cut(s) 466, 693, 1129
RseI CAYNNNNRTG 1 cut(s) 408
SacII CCGCGG 1 cut(s) 26
Sau3AI GATC 5 cut(s) 332, 444, 991, 1242, 1368
Sau96I GGNCC 1 cut(s) 1054
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 1 cut(s) 65
SduI GDGCHC 1 cut(s) 1027
SfaNI GCATC 4 cut(s) 151, 953, 1018, 1336
SfcI CTRYAG 5 cut(s) 245, 725, 824, 867, 1265
Sfr303I CCGCGG 1 cut(s) 26
SgrBI CCGCGG 1 cut(s) 26
SinI GGWCC 1 cut(s) 1054
SmiMI CAYNNNNRTG 1 cut(s) 408
SmlI CTYRAG 3 cut(s) 39, 560, 789
SmoI CTYRAG 3 cut(s) 39, 560, 789
SsiI CCGC 5 cut(s) 23, 25, 192, 1210, 1422
SspI AATATT 3 cut(s) 300, 527, 1202
SspMI CTAG 5 cut(s) 639, 840, 1293, 1359, 1436
StyD4I CCNGG 1 cut(s) 63
StyI CCWWGG 2 cut(s) 704, 1358
TaaI ACNGT 5 cut(s) 274, 771, 907, 1075, 1418
TaiI ACGT 1 cut(s) 952
TaqI TCGA 2 cut(s) 126, 1245
TatI WGTACW 3 cut(s) 465, 692, 1128
TauI GCSGC 4 cut(s) 25, 28, 194, 1424
TfiI GAWTC 3 cut(s) 5, 51, 383
TscAI CASTG 2 cut(s) 239, 1421
TseFI GTSAC 1 cut(s) 771
TseI GCWGC 8 cut(s) 517, 727, 736, 823, 847, 1003, 1006, 1399
Tsp45I GTSAC 1 cut(s) 771
TspDTI ATGAA 6 cut(s) 197, 470, 827, 1133, 1142, 1330
TspGWI ACGGA 1 cut(s) 1004
TspRI CASTG 2 cut(s) 239, 1421
VpaK11BI GGWCC 1 cut(s) 1054
XagI CCTNNNNNAGG 1 cut(s) 39
XapI RAATTY 4 cut(s) 510, 853, 982, 1149
XceI RCATGY 3 cut(s) 407, 766, 901
XmaJI CCTAGG 1 cut(s) 1358
XspI CTAG 5 cut(s) 639, 840, 1293, 1359, 1436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.