MD03G1033800.v1.1

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001201
Physical Location & Seq
Reverse (-)
2696824 .. 2701931
5108 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1033800.v1.1.491

Sequence Viewer

Length: 1590 bp
ATGGATACCAATAAGTGGAGGCCTTATCAAGTTGGAGAGGCCGCCATGGATGCCGGCGATTGGAGGAGTCAGCTGCAGGCTGATTCAAGGCAAAGAATTGTCAACAAGATAATGGATACATTGAAGAGGCATCTTCCCTTCTCCGGACAAGATGGATTACAGGAACTCAGTAAAATTGCTGTAAGGTTTGAGGAAAAGATTTATACTGCAGCCACAAGCCAGTCGGATTATCTACGGAAAATTTCTCTGAAGATGCTCACAATGGAGACCAAGTATCAGAATTCAATGGGCAATCCTTTACAATCTAACTCTGCTGGTAACAGCAACAGGCCCCCTGATCCAGGGTCTTCCGGTATGCAACCCCAAGTCCCCAATCAAGGGCAATCACACTCTATGCCGTTGCCAGCTAATCAATCTCAAGGACGCCAACCACTTTTAGCACAGAACATTCAGAATAATGTTACTGGGTTACACCAACAGCAGCAAAGCAACCTTTCTAATATCCATCAGCAACAATTGGACCTGCATAATAATGTTACTGGGTTACAGCAACAGCAGCACCTCGGAACTCAAAGTGGTAATTCTAGTATGCAACCAAATCAGCACTCTGTACACTTGTTGCAACAATCCAAGATTCAGGTGCAGCAACAACGACATCAGAATGCATCTAACATGTTACCGACTCAAGGACAGCAGTCACAACCTCAGGCATCGCAGCAACAAATGATGTCACAGATTCAATCACAGTCTGCACCGATGCAACAAATGGGTTTGCAACAGCAGTCAAATCCACTGCAACGAGATATGCAGCTTCAAGCATCAGGTCTGATATCAGGAACCATGCTTCAAGCTCAAAATATAATGGATCAGCAAAAGCAGTTATATCAGTCTCTGAGGCCCCTTTCTGAGACATCATTGACTTCTCTGGATTCCTCAGCTCAGGCTGGACATGCTAATGGGGGTGATTGGCAAGAGGAGGTCTATCAAAAAATCAAAGTCATGAAGGAAAGGTACTTACCCGAACTAAGTGAAATGTATCAGAAAATTGCTACTAAACTTCAGCAGCACGATTCTCTTCCACAACAACCAAAGTCAGAGCAGCTTGAGAAGCTTAAAATGTTCAAAACCATGTTAGAGCGCCTCATATCAGTCTTACAGATTTCCAAGAGTAGCATTTCACCTGGTTTGAAAGACAAGTTGGGTTTATATGAGAAGCAGATAGTAAATTTTATTAATACAAATGGACCACGGAAGCAAGATCCTCTGCAACAAGGGCAGCTCCCCCCACCTCATATGCACTCCATGCAGCAGCCGCAGCCTCAAACTCCTCAAGTGCAGTCTCATGAAAATCAAATGAACCCTCAGTTGCAAACAATGAATTTACAAGGTTCTGTGCCAACAATGCAGCAGAACTATATGACAAGCTTGGAGCAAAATTCAATGTCTTCGATATCTGGGGTTTCAACAGCACAACAGAACATGATGAATTCATTGCAGCTGAGTACAAATTTGGATTCAGGACAGGGAAATGCACTGAACTCTTTGCAGCAAGTTCCGGTGGGATCTATCCAACAAACTCCTTGGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

530

Amino Acids

59.29

Weight (kDa)

9.12

Isoelectric Point (pI)

67.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 19 - 98 4.1e-35 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 531
AccB7I CCANNNNNTGG 1 cut(s) 15
AccIII TCCGGA 1 cut(s) 143
AciI CCGC 2 cut(s) 42, 1313
AclWI GGATC 4 cut(s) 332, 873, 1253, 1570
AcsI RAATTY 7 cut(s) 240, 280, 1225, 1378, 1435, 1486, 1507
AcuI CTGAAG 2 cut(s) 269, 1043
AcyI GRCGYC 1 cut(s) 424
AfaI GTAC 3 cut(s) 612, 1013, 1504
AfiI CCNNNNNNNGG 7 cut(s) 15, 60, 143, 341, 377, 378, 686
AflIII ACRYGT 1 cut(s) 672
AjnI CCWGG 2 cut(s) 340, 1180
AjuI GAANNNNNNNTTGG 2 cut(s) 1181, 1213
Alw26I GTCTC 4 cut(s) 260, 894, 902, 1344
AlwI GGATC 4 cut(s) 332, 873, 1253, 1570
AlwNI CAGNNNCTG 1 cut(s) 892
Aor13HI TCCGGA 1 cut(s) 143
AoxI GGCC 4 cut(s) 20, 39, 329, 896
ApoI RAATTY 7 cut(s) 240, 280, 1225, 1378, 1435, 1486, 1507
AseI ATTAAT 1 cut(s) 1233
AspLEI GCGC 1 cut(s) 1140
AspS9I GGNCC 4 cut(s) 330, 520, 897, 1244
AsuHPI GGTGA 2 cut(s) 974, 1170
AvaII GGWCC 2 cut(s) 520, 1244
AxyI CCTNAGG 1 cut(s) 705
BarI GAAGNNNNNNTAC 2 cut(s) 995, 1027
BbsI GAAGAC 2 cut(s) 339, 1437
BbvCI CCTCAGC 1 cut(s) 934
BccI CCATC 2 cut(s) 146, 513
BceAI ACGGC 1 cut(s) 382
BciT130I CCWGG 2 cut(s) 342, 1182
BciVI GTATCC 1 cut(s) 109
BcoDI GTCTC 4 cut(s) 260, 894, 902, 1344
BfaI CTAG 1 cut(s) 585
BfmI CTRYAG 2 cut(s) 74, 207
BfoI RGCGCY 1 cut(s) 1141
BfuAI ACCTGC 1 cut(s) 531
BfuI GTATCC 1 cut(s) 109
Bme1390I CCNGG 2 cut(s) 342, 1182
Bme18I GGWCC 2 cut(s) 520, 1244
BmgT120I GGNCC 4 cut(s) 330, 520, 897, 1244
BmiI GGNNCC 3 cut(s) 332, 838, 899
BmrFI CCNGG 2 cut(s) 342, 1182
BmrI ACTGGG 2 cut(s) 474, 549
BmsI GCATC 7 cut(s) 40, 139, 243, 674, 719, 747, 827
BmuI ACTGGG 2 cut(s) 474, 549
BoxI GACNNNNGTC 1 cut(s) 694
BpiI GAAGAC 2 cut(s) 339, 1437
Bpu10I CCTNAGC 2 cut(s) 934, 939
BpuEI CTTGAG 4 cut(s) 402, 669, 1124, 1314
BsaHI GRCGYC 1 cut(s) 424
BsaI GGTCTC 1 cut(s) 260
BsaJI CCNNGG 5 cut(s) 45, 341, 562, 1247, 1580
BsaWI WCCGGW 3 cut(s) 143, 350, 1555
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bsc4I CCNNNNNNNGG 7 cut(s) 15, 60, 143, 341, 377, 378, 686
Bse118I RCCGGY 1 cut(s) 53
Bse1I ACTGG 3 cut(s) 220, 469, 544
Bse21I CCTNAGG 1 cut(s) 705
Bse3DI GCAATG 1 cut(s) 1490
BseAI TCCGGA 1 cut(s) 143
BseBI CCWGG 2 cut(s) 342, 1182
BseDI CCNNGG 5 cut(s) 45, 341, 562, 1247, 1580
BseGI GGATG 1 cut(s) 55
BseLI CCNNNNNNNGG 7 cut(s) 15, 60, 143, 341, 377, 378, 686
BseMI GCAATG 1 cut(s) 1490
BseMII CTCAG 8 cut(s) 181, 719, 884, 897, 948, 953, 1376, 1490
BseNI ACTGG 3 cut(s) 220, 469, 544
BseRI GAGGAG 3 cut(s) 79, 989, 1317
BsgI GTGCAG 3 cut(s) 662, 735, 1355
BshFI GGCC 4 cut(s) 22, 41, 331, 898
BsiSI CCGG 4 cut(s) 54, 144, 351, 1556
BslFI GGGAC 1 cut(s) 353
BslI CCNNNNNNNGG 7 cut(s) 15, 60, 143, 341, 377, 378, 686
BsmAI GTCTC 4 cut(s) 260, 894, 902, 1344
BsmFI GGGAC 1 cut(s) 353
BsmI GAATGC 1 cut(s) 667
BsnI GGCC 4 cut(s) 22, 41, 331, 898
Bso31I GGTCTC 1 cut(s) 260
Bsp13I TCCGGA 1 cut(s) 143
Bsp1407I TGTACA 1 cut(s) 610
Bsp143I GATC 4 cut(s) 337, 865, 1258, 1562
Bsp19I CCATGG 1 cut(s) 45
BspACI CCGC 2 cut(s) 42, 1313
BspANI GGCC 4 cut(s) 22, 41, 331, 898
BspCNI CTCAG 8 cut(s) 180, 718, 885, 898, 947, 952, 1375, 1491
BspEI TCCGGA 1 cut(s) 143
BspHI TCATGA 2 cut(s) 999, 1342
BspLI GGNNCC 3 cut(s) 332, 838, 899
BspMAI CTGCAG 2 cut(s) 78, 211
BspMI ACCTGC 1 cut(s) 531
BspPI GGATC 4 cut(s) 332, 873, 1253, 1570
BspTNI GGTCTC 1 cut(s) 260
BsrDI GCAATG 1 cut(s) 1490
BsrFI RCCGGY 1 cut(s) 53
BsrGI TGTACA 1 cut(s) 610
BsrI ACTGG 3 cut(s) 220, 469, 544
BssAI RCCGGY 1 cut(s) 53
BssECI CCNNGG 5 cut(s) 45, 341, 562, 1247, 1580
BssMI GATC 4 cut(s) 337, 865, 1258, 1562
BssNI GRCGYC 1 cut(s) 424
BssT1I CCWWGG 2 cut(s) 45, 1580
Bst2UI CCWGG 2 cut(s) 342, 1182
Bst4CI ACNGT 1 cut(s) 747
Bst6I CTCTTC 2 cut(s) 119, 1080
BstACI GRCGYC 1 cut(s) 424
BstAPI GCANNNNNTGC 1 cut(s) 1303
BstAUI TGTACA 1 cut(s) 610
BstC8I GCNNGC 3 cut(s) 55, 78, 405
BstDEI CTNAG 9 cut(s) 167, 705, 893, 906, 934, 939, 1025, 1362, 1499
BstDSI CCRYGG 2 cut(s) 45, 1247
BstENI CCTNNNNNAGG 1 cut(s) 339
BstF5I GGATG 1 cut(s) 55
BstH2I RGCGCY 1 cut(s) 1141
BstHHI GCGC 1 cut(s) 1140
BstKTI GATC 4 cut(s) 340, 868, 1261, 1565
BstMAI GTCTC 4 cut(s) 260, 894, 902, 1344
BstMBI GATC 4 cut(s) 337, 865, 1258, 1562
BstMWI GCNNNNNNNGC 9 cut(s) 50, 556, 950, 1108, 1273, 1303, 1312, 1315, 1402
BstNI CCWGG 2 cut(s) 342, 1182
BstNSI RCATGY 2 cut(s) 676, 953
BstPAI GACNNNNGTC 1 cut(s) 694
BstSCI CCNGG 2 cut(s) 340, 1180
BstSFI CTRYAG 2 cut(s) 74, 207
BstV2I GAAGAC 2 cut(s) 339, 1437
BstX2I RGATCY 2 cut(s) 1258, 1562
BstYI RGATCY 2 cut(s) 1258, 1562
Bsu36I CCTNAGG 1 cut(s) 705
BsuI GTATCC 1 cut(s) 109
BsuRI GGCC 4 cut(s) 22, 41, 331, 898
BtgI CCRYGG 2 cut(s) 45, 1247
BtgZI GCGATG 1 cut(s) 696
BtsCI GGATG 1 cut(s) 55
BtsI GCAGTG 1 cut(s) 791
BtsIMutI CAGTG 2 cut(s) 791, 1532
BveI ACCTGC 1 cut(s) 531
Cac8I GCNNGC 3 cut(s) 55, 78, 405
CaiI CAGNNNCTG 1 cut(s) 892
CciI TCATGA 2 cut(s) 999, 1342
CfoI GCGC 1 cut(s) 1140
Cfr10I RCCGGY 1 cut(s) 53
Cfr13I GGNCC 4 cut(s) 330, 520, 897, 1244
CseI GACGC 1 cut(s) 432
CsiI ACCWGGT 1 cut(s) 1180
Csp6I GTAC 3 cut(s) 611, 1012, 1503
CviAII CATG 9 cut(s) 46, 673, 841, 950, 1000, 1129, 1303, 1343, 1480
CviQI GTAC 3 cut(s) 611, 1012, 1503
DdeI CTNAG 9 cut(s) 167, 705, 893, 906, 934, 939, 1025, 1362, 1499
DpnI GATC 4 cut(s) 339, 867, 1260, 1564
DpnII GATC 4 cut(s) 337, 865, 1258, 1562
Eam1104I CTCTTC 2 cut(s) 119, 1080
EarI CTCTTC 2 cut(s) 119, 1080
Eco130I CCWWGG 2 cut(s) 45, 1580
Eco147I AGGCCT 1 cut(s) 22
Eco31I GGTCTC 1 cut(s) 260
Eco32I GATATC 2 cut(s) 831, 1452
Eco47I GGWCC 2 cut(s) 520, 1244
Eco57I CTGAAG 2 cut(s) 269, 1043
Eco81I CCTNAGG 1 cut(s) 705
EcoNI CCTNNNNNAGG 1 cut(s) 339
EcoO109I RGGNCCY 2 cut(s) 330, 897
EcoRI GAATTC 2 cut(s) 280, 1486
EcoRII CCWGG 2 cut(s) 340, 1180
EcoRV GATATC 2 cut(s) 831, 1452
EcoT14I CCWWGG 2 cut(s) 45, 1580
EcoT22I ATGCAT 1 cut(s) 667
ErhI CCWWGG 2 cut(s) 45, 1580
FaeI CATG 9 cut(s) 49, 676, 844, 953, 1003, 1132, 1306, 1346, 1483
FaqI GGGAC 1 cut(s) 353
FatI CATG 9 cut(s) 45, 672, 840, 949, 999, 1128, 1302, 1342, 1479
FauNDI CATATG 1 cut(s) 1293
FokI GGATG 1 cut(s) 62
FspBI CTAG 1 cut(s) 585
GlaI GCGC 1 cut(s) 1139
HaeII RGCGCY 1 cut(s) 1141
HaeIII GGCC 4 cut(s) 22, 41, 331, 898
HapII CCGG 4 cut(s) 54, 144, 351, 1556
HgaI GACGC 1 cut(s) 432
HhaI GCGC 1 cut(s) 1140
Hin1I GRCGYC 1 cut(s) 424
Hin1II CATG 9 cut(s) 49, 676, 844, 953, 1003, 1132, 1306, 1346, 1483
Hin6I GCGC 1 cut(s) 1138
HinP1I GCGC 1 cut(s) 1138
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HindIII AAGCTT 2 cut(s) 1109, 1423
HinfI GANTC 8 cut(s) 67, 83, 634, 682, 736, 929, 1070, 1514
HpaII CCGG 4 cut(s) 54, 144, 351, 1556
HphI GGTGA 2 cut(s) 974, 1170
Hpy166II GTNNAC 2 cut(s) 103, 613
Hpy188III TCNNGA 6 cut(s) 144, 834, 926, 1000, 1343, 1518
Hpy8I GTNNAC 2 cut(s) 103, 613
HpyAV CCTTC 2 cut(s) 148, 997
HpyCH4III ACNGT 1 cut(s) 747
HpyF10VI GCNNNNNNNGC 9 cut(s) 50, 556, 950, 1108, 1273, 1303, 1312, 1315, 1402
HpyF3I CTNAG 9 cut(s) 167, 705, 893, 906, 934, 939, 1025, 1362, 1499
Hsp92I GRCGYC 1 cut(s) 424
Hsp92II CATG 9 cut(s) 49, 676, 844, 953, 1003, 1132, 1306, 1346, 1483
HspAI GCGC 1 cut(s) 1138
Kpn2I TCCGGA 1 cut(s) 143
KroI GCCGGC 1 cut(s) 53
KroNI GCCGGC 1 cut(s) 55
Kzo9I GATC 4 cut(s) 337, 865, 1258, 1562
LmnI GCTCC 2 cut(s) 1284, 1429
LweI GCATC 7 cut(s) 40, 139, 243, 674, 719, 747, 827
MabI ACCWGGT 1 cut(s) 1180
MaeI CTAG 1 cut(s) 585
MaeIII GTNAC 8 cut(s) 317, 460, 468, 535, 543, 675, 696, 729
MalI GATC 4 cut(s) 339, 867, 1260, 1564
MboI GATC 4 cut(s) 337, 865, 1258, 1562
MboII GAAGA 6 cut(s) 125, 136, 262, 339, 1067, 1437
MfeI CAATTG 1 cut(s) 515
MflI RGATCY 2 cut(s) 1258, 1562
MlyI GAGTC 2 cut(s) 76, 676
MmeI TCCRAC 2 cut(s) 13, 204
Mph1103I ATGCAT 1 cut(s) 667
MroI TCCGGA 1 cut(s) 143
MroNI GCCGGC 1 cut(s) 53
MseI TTAA 2 cut(s) 1113, 1233
MslI CAYNNNNRTG 3 cut(s) 531, 660, 954
MspA1I CMGCKG 2 cut(s) 73, 1498
MspI CCGG 4 cut(s) 54, 144, 351, 1556
MspR9I CCNGG 2 cut(s) 342, 1182
MunI CAATTG 1 cut(s) 515
Mva1269I GAATGC 1 cut(s) 667
MvaI CCWGG 2 cut(s) 342, 1182
MwoI GCNNNNNNNGC 9 cut(s) 50, 556, 950, 1108, 1273, 1303, 1312, 1315, 1402
NaeI GCCGGC 1 cut(s) 55
NcoI CCATGG 1 cut(s) 45
NdeI CATATG 1 cut(s) 1293
NdeII GATC 4 cut(s) 337, 865, 1258, 1562
NgoMIV GCCGGC 1 cut(s) 53
NlaIII CATG 9 cut(s) 49, 676, 844, 953, 1003, 1132, 1306, 1346, 1483
NlaIV GGNNCC 3 cut(s) 332, 838, 899
NmuCI GTSAC 2 cut(s) 696, 729
NsiI ATGCAT 1 cut(s) 667
NspI RCATGY 2 cut(s) 676, 953
PagI TCATGA 2 cut(s) 999, 1342
PceI AGGCCT 1 cut(s) 22
PciI ACATGT 1 cut(s) 672
PctI GAATGC 1 cut(s) 667
PdiI GCCGGC 1 cut(s) 55
PfeI GAWTC 6 cut(s) 83, 634, 736, 929, 1070, 1514
PflMI CCANNNNNTGG 1 cut(s) 15
PleI GAGTC 2 cut(s) 75, 676
PpsI GAGTC 2 cut(s) 75, 676
PscI ACATGT 1 cut(s) 672
PshAI GACNNNNGTC 1 cut(s) 694
PshBI ATTAAT 1 cut(s) 1233
Psp6I CCWGG 2 cut(s) 340, 1180
PspGI CCWGG 2 cut(s) 340, 1180
PspN4I GGNNCC 3 cut(s) 332, 838, 899
PspPI GGNCC 4 cut(s) 330, 520, 897, 1244
PstI CTGCAG 2 cut(s) 78, 211
PstNI CAGNNNCTG 1 cut(s) 892
PsuI RGATCY 2 cut(s) 1258, 1562
PvuII CAGCTG 2 cut(s) 73, 1498
RsaI GTAC 3 cut(s) 612, 1013, 1504
RsaNI GTAC 3 cut(s) 611, 1012, 1503
RseI CAYNNNNRTG 3 cut(s) 531, 660, 954
SaqAI TTAA 2 cut(s) 1113, 1233
Sau3AI GATC 4 cut(s) 337, 865, 1258, 1562
Sau96I GGNCC 4 cut(s) 330, 520, 897, 1244
SchI GAGTC 2 cut(s) 76, 676
ScrFI CCNGG 2 cut(s) 342, 1182
SexAI ACCWGGT 1 cut(s) 1180
SfaNI GCATC 7 cut(s) 40, 139, 243, 674, 719, 747, 827
SfcI CTRYAG 2 cut(s) 74, 207
SinI GGWCC 2 cut(s) 520, 1244
SmiMI CAYNNNNRTG 3 cut(s) 531, 660, 954
SmlI CTYRAG 4 cut(s) 417, 684, 1103, 1329
SmoI CTYRAG 4 cut(s) 417, 684, 1103, 1329
SseBI AGGCCT 1 cut(s) 22
SsiI CCGC 2 cut(s) 42, 1313
SspMI CTAG 1 cut(s) 585
StuI AGGCCT 1 cut(s) 22
StyD4I CCNGG 2 cut(s) 340, 1180
StyI CCWWGG 2 cut(s) 45, 1580
TaaI ACNGT 1 cut(s) 747
TaqI TCGA 1 cut(s) 1448
TatI WGTACW 2 cut(s) 610, 1502
TauI GCSGC 2 cut(s) 44, 1315
TfiI GAWTC 6 cut(s) 83, 634, 736, 929, 1070, 1514
Tru1I TTAA 2 cut(s) 1113, 1233
Tru9I TTAA 2 cut(s) 1113, 1233
TscAI CASTG 2 cut(s) 798, 1539
TseFI GTSAC 2 cut(s) 696, 729
Tsp45I GTSAC 2 cut(s) 696, 729
TspDTI ATGAA 6 cut(s) 1016, 1359, 1370, 1391, 1479, 1499
TspGWI ACGGA 2 cut(s) 250, 1264
TspRI CASTG 2 cut(s) 798, 1539
Van91I CCANNNNNTGG 1 cut(s) 15
VpaK11BI GGWCC 2 cut(s) 520, 1244
VspI ATTAAT 1 cut(s) 1233
XagI CCTNNNNNAGG 1 cut(s) 339
XapI RAATTY 7 cut(s) 240, 280, 1225, 1378, 1435, 1486, 1507
XceI RCATGY 2 cut(s) 676, 953
XspI CTAG 1 cut(s) 585
Zsp2I ATGCAT 1 cut(s) 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.