pycom03g02630

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0001201
Physical Location & Seq
Reverse (-)
2010441 .. 2012427
1987 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g02630.2

Sequence Viewer

Length: 1533 bp
ATGAAGGAAAAGTACTTACCCGAACTAAGTGAAATGTATCACAAAATTGCTACTAAACTTCAGCAGCACGATTCTCTTCCACAACAACCAAAGTCAGAGCATCTTGAGAAGCTAAAAATGTTCAAAACCATGTTAGAGCGCCTCATATCAATCTTACAGGTTTCCAAGAGTAACATTTCACCTGGTTTGAAAGACAAGTTGGGTTTATATGAGAAGCAGATAGTAAATTTTATTAATACAAATAGACCGAGGAAGCAAGGTCCTCCTCTGCAACAAGGGCAGCTCACCCCACCTCATTTGCACTCCATGCACCAACCGCAGTCTCAAATTCCTCAAGTGCTGTCTCATGAAAATCAAATGAACCCGCAGTTGCAAACAATGAATTTACAAGGTTCTGTGCCAACAATGCAGCAGAACAATATGACAAGTTTGCAGCAAAATTCAATGTCTTCGATATCTGGGGTTTCAACAGCACAGCAGAACATGATGAATTCATTGCAGCCGAGTACAAATTTGGATTCAGGACAGGGAAATGCACTGAACTCTTTGCAGCAAGTTCTGGTGGGATCTATCCAACAAACTCCTGTCAGTGCTCCCCAGCTAGCTAACATGAATGGTTTGTCATCACAGAGTGGGGTTAGTATGCTTCAGCCAAATATTAACTCCCTTCAGTCAAATTCTGGTATGCTTCAACACCAGCAATTAAAACAGCAGCAGCATGTGATACAAAAGCAGCTGCAGCAACAACAGCAGCAGCAGCAACCGCAGCAACAGTTGCAGCAAGCAAAGCAGCAGCTTCCGGCGCAGATGCAGGCGAACCAACACCAAATGCCACAGCTTCATCAAATGAATGATGCAAATGACTTGAAGATGAGACAGGGGATGGGTGTTAAGCCAGGGGTTTTTCAGCAACATATGTCCTCAGGCCAGCGTGCTTATCCTCATCAGCAGTTGAAATCAGGATCTCCATTTCCTATTCCTTCATCAAACCAACTCCTTCAGGCTGCATCTCCTCAAATTTCGCAACATTCTCCTCCCCAAGTTGACCACATGAATCTACTGACTCGCCCGAAAGCTGGAACACTATTGCAAACTGCCGGTTCACCTTTTGTCATTCCATCTCCTTCAACTCCCATGGCTCCATCCCCCATGCCATGGGATTCTGAGAAACCTTCCTCACTATCAAATGCTGGGAATATCGGACATCAACAAGCAGCTAGGGTGGGAGCACCAGTCCAGTCTTTTGCAATTGGCAGCCCTGGGATATCAGCATCTCCTTTGCTTGCTGAATTTAGTGTACCAGATGGTAGTCATGTTAATGTTTTGTCAACTATTTCTGCCAAGTCGAGTGTTACTGAGCAGCCTCTTGAACGCTTGATTAAGGCGGAGAAATCGATGTCACCTAATGCTTTGAGTGCATCTGTCAGTGACATTGACTCAGATGCATTGAGTGCATCTGTTTGCTGCTGTGAGGTGGAAGGGTGGGCGTTGTCATCATTGTGGTGCTGCTACTCCCGCCGCCGCTGCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

511

Amino Acids

55.87

Weight (kDa)

9.18

Isoelectric Point (pI)

72.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1155
AciI CCGC 7 cut(s) 317, 365, 764, 1385, 1516, 1519, 1522
AclWI GGATC 2 cut(s) 574, 970
AcsI RAATTY 9 cut(s) 226, 327, 382, 439, 490, 511, 676, 1017, 1289
AcuI CTGAAG 4 cut(s) 44, 632, 653, 983
AdeI CACNNNGTG 1 cut(s) 632
AfaI GTAC 3 cut(s) 14, 508, 1299
AfiI CCNNNNNNNGG 2 cut(s) 1076, 1155
AgsI TTSAA 9 cut(s) 124, 190, 444, 468, 692, 868, 955, 1128, 1370
AjnI CCWGG 3 cut(s) 181, 895, 1258
AjuI GAANNNNNNNTTGG 2 cut(s) 182, 214
AluBI AGCT 9 cut(s) 112, 283, 601, 605, 736, 796, 838, 1076, 1217
AluI AGCT 9 cut(s) 112, 283, 601, 605, 736, 796, 838, 1076, 1217
Alw21I GWGCWC 2 cut(s) 595, 1231
Alw26I GTCTC 3 cut(s) 327, 348, 868
AlwI GGATC 2 cut(s) 574, 970
AoxI GGCC 1 cut(s) 925
ApoI RAATTY 9 cut(s) 226, 327, 382, 439, 490, 511, 676, 1017, 1289
AseI ATTAAT 1 cut(s) 234
AspLEI GCGC 2 cut(s) 141, 805
AspS9I GGNCC 1 cut(s) 260
AsuHPI GGTGA 4 cut(s) 171, 277, 1095, 1392
AsuNHI GCTAGC 1 cut(s) 601
AvaII GGWCC 1 cut(s) 260
AxyI CCTNAGG 1 cut(s) 922
BarI GAAGNNNNNNTAC 1 cut(s) 28
BbsI GAAGAC 1 cut(s) 441
Bbv12I GWGCWC 2 cut(s) 595, 1231
BccI CCATC 4 cut(s) 877, 1126, 1150, 1298
BciT130I CCWGG 3 cut(s) 183, 897, 1260
BcoDI GTCTC 3 cut(s) 327, 348, 868
BfaI CTAG 3 cut(s) 602, 1218, 1531
BfmI CTRYAG 1 cut(s) 737
BfoI RGCGCY 1 cut(s) 142
BmcAI AGTACT 1 cut(s) 14
Bme1390I CCNGG 3 cut(s) 183, 897, 1260
Bme18I GGWCC 1 cut(s) 260
BmgT120I GGNCC 1 cut(s) 260
BmiI GGNNCC 1 cut(s) 1140
BmrFI CCNGG 3 cut(s) 183, 897, 1260
BmsI GCATC 8 cut(s) 109, 798, 844, 1016, 1280, 1427, 1432, 1463
BmtI GCTAGC 1 cut(s) 605
BpiI GAAGAC 1 cut(s) 441
BpuEI CTTGAG 2 cut(s) 125, 318
Bsa29I ATCGAT 1 cut(s) 1394
BsaJI CCNNGG 6 cut(s) 248, 896, 1134, 1154, 1258, 1259
BsaXI ACNNNNNCTCC 2 cut(s) 1218, 1248
Bsc4I CCNNNNNNNGG 2 cut(s) 1076, 1155
Bse118I RCCGGY 1 cut(s) 1097
Bse1I ACTGG 2 cut(s) 1232, 1237
Bse21I CCTNAGG 1 cut(s) 922
Bse3DI GCAATG 1 cut(s) 494
BseBI CCWGG 3 cut(s) 183, 897, 1260
BseCI ATCGAT 1 cut(s) 1394
BseDI CCNNGG 6 cut(s) 248, 896, 1134, 1154, 1258, 1259
BseGI GGATG 2 cut(s) 888, 1142
BseLI CCNNNNNNNGG 2 cut(s) 1076, 1155
BseMI GCAATG 1 cut(s) 494
BseMII CTCAG 4 cut(s) 936, 1155, 1347, 1452
BseNI ACTGG 2 cut(s) 1232, 1237
BseRI GAGGAG 3 cut(s) 255, 1002, 1023
BseYI CCCAGC 2 cut(s) 597, 1190
BshFI GGCC 1 cut(s) 927
BshVI ATCGAT 1 cut(s) 1394
BsiHKAI GWGCWC 2 cut(s) 595, 1231
BsiSI CCGG 2 cut(s) 800, 1098
BslI CCNNNNNNNGG 2 cut(s) 1076, 1155
BsmAI GTCTC 3 cut(s) 327, 348, 868
BsnI GGCC 1 cut(s) 927
Bsp1286I GDGCHC 2 cut(s) 595, 1231
Bsp143I GATC 2 cut(s) 566, 962
Bsp19I CCATGG 2 cut(s) 1134, 1154
BspACI CCGC 7 cut(s) 317, 365, 764, 1385, 1516, 1519, 1522
BspANI GGCC 1 cut(s) 927
BspCNI CTCAG 4 cut(s) 935, 1156, 1348, 1451
BspDI ATCGAT 1 cut(s) 1394
BspHI TCATGA 1 cut(s) 346
BspLI GGNNCC 1 cut(s) 1140
BspMAI CTGCAG 1 cut(s) 741
BspOI GCTAGC 1 cut(s) 605
BspPI GGATC 2 cut(s) 574, 970
BsrDI GCAATG 1 cut(s) 494
BsrFI RCCGGY 1 cut(s) 1097
BsrI ACTGG 2 cut(s) 1232, 1237
BssAI RCCGGY 1 cut(s) 1097
BssECI CCNNGG 6 cut(s) 248, 896, 1134, 1154, 1258, 1259
BssMI GATC 2 cut(s) 566, 962
BssT1I CCWWGG 2 cut(s) 1134, 1154
Bst2UI CCWGG 3 cut(s) 183, 897, 1260
Bst4CI ACNGT 1 cut(s) 774
Bst6I CTCTTC 1 cut(s) 81
BstAPI GCANNNNNTGC 3 cut(s) 307, 775, 1451
BstC8I GCNNGC 6 cut(s) 603, 783, 813, 929, 933, 1284
BstDEI CTNAG 5 cut(s) 26, 922, 1164, 1356, 1438
BstDSI CCRYGG 2 cut(s) 1134, 1154
BstF5I GGATG 2 cut(s) 888, 1142
BstH2I RGCGCY 1 cut(s) 142
BstHHI GCGC 2 cut(s) 141, 805
BstKTI GATC 2 cut(s) 569, 965
BstMAI GTCTC 3 cut(s) 327, 348, 868
BstMBI GATC 2 cut(s) 566, 962
BstNI CCWGG 3 cut(s) 183, 897, 1260
BstNSI RCATGY 1 cut(s) 722
BstSCI CCNGG 3 cut(s) 181, 895, 1258
BstSFI CTRYAG 1 cut(s) 737
BstV2I GAAGAC 1 cut(s) 441
BstX2I RGATCY 2 cut(s) 566, 962
BstYI RGATCY 2 cut(s) 566, 962
Bsu15I ATCGAT 1 cut(s) 1394
Bsu36I CCTNAGG 1 cut(s) 922
BsuRI GGCC 1 cut(s) 927
BsuTUI ATCGAT 1 cut(s) 1394
BtgI CCRYGG 2 cut(s) 1134, 1154
BtsCI GGATG 2 cut(s) 888, 1142
BtsIMutI CAGTG 3 cut(s) 536, 595, 1432
Cac8I GCNNGC 6 cut(s) 603, 783, 813, 929, 933, 1284
CciI TCATGA 1 cut(s) 346
CfoI GCGC 2 cut(s) 141, 805
Cfr10I RCCGGY 1 cut(s) 1097
Cfr13I GGNCC 1 cut(s) 260
ClaI ATCGAT 1 cut(s) 1394
CsiI ACCWGGT 1 cut(s) 181
Csp6I GTAC 3 cut(s) 13, 507, 1298
CspCI CAANNNNNGTGG 2 cut(s) 279, 314
CviQI GTAC 3 cut(s) 13, 507, 1298
DdeI CTNAG 5 cut(s) 26, 922, 1164, 1356, 1438
DpnI GATC 2 cut(s) 568, 964
DpnII GATC 2 cut(s) 566, 962
DraIII CACNNNGTG 1 cut(s) 632
Eam1104I CTCTTC 1 cut(s) 81
EarI CTCTTC 1 cut(s) 81
EciI GGCGGA 1 cut(s) 1400
Eco130I CCWWGG 2 cut(s) 1134, 1154
Eco32I GATATC 2 cut(s) 456, 1266
Eco47I GGWCC 1 cut(s) 260
Eco57I CTGAAG 4 cut(s) 44, 632, 653, 983
Eco81I CCTNAGG 1 cut(s) 922
EcoO109I RGGNCCY 1 cut(s) 260
EcoRI GAATTC 1 cut(s) 490
EcoRII CCWGG 3 cut(s) 181, 895, 1258
EcoRV GATATC 2 cut(s) 456, 1266
EcoT14I CCWWGG 2 cut(s) 1134, 1154
EcoT22I ATGCAT 1 cut(s) 1447
ErhI CCWWGG 2 cut(s) 1134, 1154
FauI CCCGC 2 cut(s) 372, 1523
FauNDI CATATG 1 cut(s) 915
FokI GGATG 2 cut(s) 895, 1129
FspBI CTAG 3 cut(s) 602, 1218, 1531
GlaI GCGC 2 cut(s) 140, 804
GsaI CCCAGC 2 cut(s) 601, 1194
HaeII RGCGCY 1 cut(s) 142
HaeIII GGCC 1 cut(s) 927
HapII CCGG 2 cut(s) 800, 1098
HhaI GCGC 2 cut(s) 141, 805
Hin6I GCGC 2 cut(s) 139, 803
HinP1I GCGC 2 cut(s) 139, 803
HincII GTYRAC 2 cut(s) 1045, 1329
HindII GTYRAC 2 cut(s) 1045, 1329
HinfI GANTC 6 cut(s) 71, 518, 1054, 1063, 1160, 1436
HpaII CCGG 2 cut(s) 800, 1098
HphI GGTGA 4 cut(s) 171, 277, 1095, 1392
Hpy166II GTNNAC 4 cut(s) 1045, 1103, 1298, 1329
Hpy188I TCNGA 4 cut(s) 97, 1165, 1202, 1441
Hpy188III TCNNGA 5 cut(s) 104, 347, 522, 960, 1367
Hpy8I GTNNAC 4 cut(s) 1045, 1103, 1298, 1329
HpyAV CCTTC 6 cut(s) 677, 990, 1007, 1134, 1182, 1472
HpyCH4III ACNGT 1 cut(s) 774
HpyF3I CTNAG 5 cut(s) 26, 922, 1164, 1356, 1438
HspAI GCGC 2 cut(s) 139, 803
Kzo9I GATC 2 cut(s) 566, 962
LmnI GCTCC 3 cut(s) 598, 1144, 1226
LweI GCATC 8 cut(s) 109, 798, 844, 1016, 1280, 1427, 1432, 1463
MabI ACCWGGT 1 cut(s) 181
MaeI CTAG 3 cut(s) 602, 1218, 1531
MaeIII GTNAC 4 cut(s) 170, 1351, 1398, 1427
MalI GATC 2 cut(s) 568, 964
MboI GATC 2 cut(s) 566, 962
MboII GAAGA 3 cut(s) 68, 441, 880
MfeI CAATTG 1 cut(s) 1248
MflI RGATCY 2 cut(s) 566, 962
MhlI GDGCHC 2 cut(s) 595, 1231
MlyI GAGTC 2 cut(s) 1057, 1430
MmeI TCCRAC 1 cut(s) 598
Mph1103I ATGCAT 1 cut(s) 1447
MseI TTAA 6 cut(s) 234, 660, 704, 891, 1317, 1380
MslI CAYNNNNRTG 3 cut(s) 1317, 1498, 1501
MspA1I CMGCKG 2 cut(s) 736, 1524
MspI CCGG 2 cut(s) 800, 1098
MspR9I CCNGG 3 cut(s) 183, 897, 1260
MunI CAATTG 1 cut(s) 1248
MvaI CCWGG 3 cut(s) 183, 897, 1260
NcoI CCATGG 2 cut(s) 1134, 1154
NdeI CATATG 1 cut(s) 915
NdeII GATC 2 cut(s) 566, 962
NheI GCTAGC 1 cut(s) 601
NlaIV GGNNCC 1 cut(s) 1140
NmeAIII GCCGAG 1 cut(s) 528
NmuCI GTSAC 2 cut(s) 1398, 1427
NsiI ATGCAT 1 cut(s) 1447
NspI RCATGY 1 cut(s) 722
PagI TCATGA 1 cut(s) 346
PasI CCCWGGG 1 cut(s) 1259
PfeI GAWTC 4 cut(s) 71, 518, 1054, 1160
PflMI CCANNNNNTGG 1 cut(s) 1155
PleI GAGTC 2 cut(s) 1057, 1430
PpsI GAGTC 2 cut(s) 1057, 1430
PpuMI RGGWCCY 1 cut(s) 260
PshBI ATTAAT 1 cut(s) 234
Psp5II RGGWCCY 1 cut(s) 260
Psp6I CCWGG 3 cut(s) 181, 895, 1258
PspFI CCCAGC 2 cut(s) 597, 1190
PspGI CCWGG 3 cut(s) 181, 895, 1258
PspN4I GGNNCC 1 cut(s) 1140
PspPI GGNCC 1 cut(s) 260
PspPPI RGGWCCY 1 cut(s) 260
PstI CTGCAG 1 cut(s) 741
PsuI RGATCY 2 cut(s) 566, 962
PvuII CAGCTG 1 cut(s) 736
RsaI GTAC 3 cut(s) 14, 508, 1299
RsaNI GTAC 3 cut(s) 13, 507, 1298
RseI CAYNNNNRTG 3 cut(s) 1317, 1498, 1501
SaqAI TTAA 6 cut(s) 234, 660, 704, 891, 1317, 1380
Sau3AI GATC 2 cut(s) 566, 962
Sau96I GGNCC 1 cut(s) 260
ScaI AGTACT 1 cut(s) 14
SchI GAGTC 2 cut(s) 1057, 1430
ScrFI CCNGG 3 cut(s) 183, 897, 1260
SduI GDGCHC 2 cut(s) 595, 1231
SexAI ACCWGGT 1 cut(s) 181
SfaNI GCATC 8 cut(s) 109, 798, 844, 1016, 1280, 1427, 1432, 1463
SfcI CTRYAG 1 cut(s) 737
SinI GGWCC 1 cut(s) 260
SmiMI CAYNNNNRTG 3 cut(s) 1317, 1498, 1501
SmlI CTYRAG 2 cut(s) 104, 333
SmoI CTYRAG 2 cut(s) 104, 333
SsiI CCGC 7 cut(s) 317, 365, 764, 1385, 1516, 1519, 1522
SspI AATATT 1 cut(s) 658
SspMI CTAG 3 cut(s) 602, 1218, 1531
StyD4I CCNGG 3 cut(s) 181, 895, 1258
StyI CCWWGG 2 cut(s) 1134, 1154
TaaI ACNGT 1 cut(s) 774
TaqI TCGA 3 cut(s) 452, 1346, 1394
TaqII GACCGA 1 cut(s) 262
TatI WGTACW 2 cut(s) 12, 506
TauI GCSGC 2 cut(s) 1521, 1524
TfiI GAWTC 4 cut(s) 71, 518, 1054, 1160
Tru1I TTAA 6 cut(s) 234, 660, 704, 891, 1317, 1380
Tru9I TTAA 6 cut(s) 234, 660, 704, 891, 1317, 1380
TscAI CASTG 3 cut(s) 543, 595, 1432
TseFI GTSAC 2 cut(s) 1398, 1427
Tsp45I GTSAC 2 cut(s) 1398, 1427
TspRI CASTG 3 cut(s) 543, 595, 1432
Van91I CCANNNNNTGG 1 cut(s) 1155
VpaK11BI GGWCC 1 cut(s) 260
VspI ATTAAT 1 cut(s) 234
XapI RAATTY 9 cut(s) 226, 327, 382, 439, 490, 511, 676, 1017, 1289
XceI RCATGY 1 cut(s) 722
XspI CTAG 3 cut(s) 602, 1218, 1531
ZrmI AGTACT 1 cut(s) 14
Zsp2I ATGCAT 1 cut(s) 1447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.