Rh5CG545100

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0001201
Physical Location & Seq
Forward (+)
76056466 .. 76058931
2466 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG545100.1

Sequence Viewer

Length: 627 bp
ATGGACGCGAATGGTGCTGATTGGCAAGAGGAGGTCTATCAAAAGATCAAAGTCATGAAGGAGATGTACTACTCCGAACTAAGTGAAATGTACCCAAAACTTGCTACTAAAATTCATCAGTACGAGTCCCTTCCACAACAGCCAATGTCAGATCGGCTTGAGAAGCTGAAGATGTTTAAGACCTTGCTGGAGCACCTGATTGCTGTCTTACAAATTTCCAAAACTAACATATCACCTGGTTTGAAGGAGAAGTTGGATTTATATGAGAAGCAGATGATAAATTTTATTAATGTAAATAGACCCAGGACTACTCAAGTGCAATCTCATGAAAATCAGATGATCCTACAATTGCAATCCCTGAACTTGCAAGGTTCCGTGCCAACAATGCAGCAGAACAATATGACGGGCTTGCAGCAAAATTCTATGTCTTCTTTATCTGGGGTTTCAACAGCACAGCAAAACATGATGAATCGATTGCAGCCAAGTTCCAATATTTATCCAGGACAAGGAACCGCACTGAACTCACTGCAGCAGGTTCCTGTGGGCTCTGTCCAACAAGCTTCTGTCAGTGCTCCCCAACAAGTTAACATGAATGCCTTACCATCACAGAATGGGATTAACATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

208

Amino Acids

23.48

Weight (kDa)

7.9

Isoelectric Point (pI)

55.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 523
AccII CGCG 1 cut(s) 8
AciI CCGC 1 cut(s) 513
AclWI GGATC 1 cut(s) 334
AcsI RAATTY 4 cut(s) 111, 213, 280, 418
AcuI CTGAAG 1 cut(s) 188
AfaI GTAC 3 cut(s) 68, 92, 122
AfiI CCNNNNNNNGG 1 cut(s) 506
AflIII ACRYGT 1 cut(s) 621
AgsI TTSAA 2 cut(s) 244, 447
AjnI CCWGG 3 cut(s) 235, 302, 499
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AluBI AGCT 2 cut(s) 166, 560
AluI AGCT 2 cut(s) 166, 560
Alw21I GWGCWC 2 cut(s) 195, 574
AlwI GGATC 1 cut(s) 334
ApeKI GCWGC 4 cut(s) 388, 412, 478, 529
ApoI RAATTY 4 cut(s) 111, 213, 280, 418
AseI ATTAAT 1 cut(s) 288
AsuHPI GGTGA 1 cut(s) 225
BanII GRGCYC 1 cut(s) 548
BarI GAAGNNNNNNTAC 2 cut(s) 50, 82
BbsI GAAGAC 1 cut(s) 420
Bbv12I GWGCWC 2 cut(s) 195, 574
BbvI GCAGC 4 cut(s) 400, 424, 490, 541
BccI CCATC 1 cut(s) 610
BciT130I CCWGG 3 cut(s) 237, 304, 501
BfmI CTRYAG 1 cut(s) 527
BfuAI ACCTGC 1 cut(s) 523
BisI GCNGC 4 cut(s) 389, 413, 479, 530
BlsI GCNGC 4 cut(s) 390, 414, 480, 531
Bme1390I CCNGG 3 cut(s) 237, 304, 501
BmiI GGNNCC 3 cut(s) 373, 511, 537
BmrFI CCNGG 3 cut(s) 237, 304, 501
BpiI GAAGAC 1 cut(s) 420
BpmI CTGGAG 1 cut(s) 209
BpuEI CTTGAG 2 cut(s) 179, 297
Bsa29I ATCGAT 1 cut(s) 472
BsaJI CCNNGG 1 cut(s) 302
Bsc4I CCNNNNNNNGG 1 cut(s) 506
BseBI CCWGG 3 cut(s) 237, 304, 501
BseCI ATCGAT 1 cut(s) 472
BseDI CCNNGG 1 cut(s) 302
BseLI CCNNNNNNNGG 1 cut(s) 506
BseRI GAGGAG 1 cut(s) 44
BseXI GCAGC 4 cut(s) 400, 424, 490, 541
Bsh1236I CGCG 1 cut(s) 8
BshVI ATCGAT 1 cut(s) 472
BsiHKAI GWGCWC 2 cut(s) 195, 574
BslFI GGGAC 1 cut(s) 112
BslI CCNNNNNNNGG 1 cut(s) 506
BsmFI GGGAC 1 cut(s) 112
BsmI GAATGC 1 cut(s) 598
Bsp1286I GDGCHC 3 cut(s) 195, 548, 574
Bsp143I GATC 3 cut(s) 45, 151, 339
BspACI CCGC 1 cut(s) 513
BspDI ATCGAT 1 cut(s) 472
BspFNI CGCG 1 cut(s) 8
BspHI TCATGA 2 cut(s) 54, 325
BspLI GGNNCC 3 cut(s) 373, 511, 537
BspMAI CTGCAG 1 cut(s) 531
BspMI ACCTGC 1 cut(s) 523
BspPI GGATC 1 cut(s) 334
BssECI CCNNGG 1 cut(s) 302
BssMI GATC 3 cut(s) 45, 151, 339
Bst2UI CCWGG 3 cut(s) 237, 304, 501
BstC8I GCNNGC 1 cut(s) 410
BstDEI CTNAG 1 cut(s) 80
BstFNI CGCG 1 cut(s) 8
BstKTI GATC 3 cut(s) 48, 154, 342
BstMBI GATC 3 cut(s) 45, 151, 339
BstMWI GCNNNNNNNGC 3 cut(s) 14, 163, 385
BstNI CCWGG 3 cut(s) 237, 304, 501
BstNSI RCATGY 1 cut(s) 625
BstSCI CCNGG 3 cut(s) 235, 302, 499
BstSFI CTRYAG 1 cut(s) 527
BstUI CGCG 1 cut(s) 8
BstV1I GCAGC 4 cut(s) 400, 424, 490, 541
BstV2I GAAGAC 1 cut(s) 420
Bsu15I ATCGAT 1 cut(s) 472
BsuTUI ATCGAT 1 cut(s) 472
BtsI GCAGTG 1 cut(s) 524
BtsIMutI CAGTG 3 cut(s) 515, 524, 574
BveI ACCTGC 1 cut(s) 523
Cac8I GCNNGC 1 cut(s) 410
CciI TCATGA 2 cut(s) 54, 325
ClaI ATCGAT 1 cut(s) 472
CseI GACGC 1 cut(s) 14
CsiI ACCWGGT 1 cut(s) 235
Csp6I GTAC 3 cut(s) 67, 91, 121
CviAII CATG 5 cut(s) 55, 326, 463, 589, 622
CviJI RGCY 7 cut(s) 142, 157, 166, 408, 481, 546, 560
CviKI_1 RGCY 7 cut(s) 142, 157, 166, 408, 481, 546, 560
CviQI GTAC 3 cut(s) 67, 91, 121
DdeI CTNAG 1 cut(s) 80
DpnI GATC 3 cut(s) 47, 153, 341
DpnII GATC 3 cut(s) 45, 151, 339
Eco24I GRGCYC 1 cut(s) 548
Eco57I CTGAAG 1 cut(s) 188
EcoRII CCWGG 3 cut(s) 235, 302, 499
EcoT38I GRGCYC 1 cut(s) 548
FaeI CATG 5 cut(s) 58, 329, 466, 592, 625
FaqI GGGAC 1 cut(s) 112
FatI CATG 5 cut(s) 54, 325, 462, 588, 621
Fnu4HI GCNGC 4 cut(s) 389, 413, 479, 530
FriOI GRGCYC 1 cut(s) 548
Fsp4HI GCNGC 4 cut(s) 389, 413, 479, 530
GluI GCNGC 4 cut(s) 389, 413, 479, 530
GsuI CTGGAG 1 cut(s) 209
HgaI GACGC 1 cut(s) 14
Hin1II CATG 5 cut(s) 58, 329, 466, 592, 625
HincII GTYRAC 1 cut(s) 586
HindII GTYRAC 1 cut(s) 586
HindIII AAGCTT 1 cut(s) 558
HinfI GANTC 2 cut(s) 125, 469
HpaI GTTAAC 1 cut(s) 586
HphI GGTGA 1 cut(s) 225
Hpy166II GTNNAC 1 cut(s) 586
Hpy188I TCNGA 3 cut(s) 76, 151, 336
Hpy188III TCNNGA 2 cut(s) 55, 326
Hpy8I GTNNAC 1 cut(s) 586
HpyAV CCTTC 3 cut(s) 52, 140, 238
HpyCH4V TGCA 7 cut(s) 319, 352, 367, 388, 412, 478, 529
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 163, 385
HpyF3I CTNAG 1 cut(s) 80
Hsp92II CATG 5 cut(s) 58, 329, 466, 592, 625
KspAI GTTAAC 1 cut(s) 586
Kzo9I GATC 3 cut(s) 45, 151, 339
LmnI GCTCC 2 cut(s) 190, 577
Lsp1109I GCAGC 4 cut(s) 400, 424, 490, 541
MabI ACCWGGT 1 cut(s) 235
MalI GATC 3 cut(s) 47, 153, 341
MboI GATC 3 cut(s) 45, 151, 339
MboII GAAGA 2 cut(s) 181, 420
MfeI CAATTG 1 cut(s) 347
MhlI GDGCHC 3 cut(s) 195, 548, 574
MluCI AATT 5 cut(s) 111, 213, 280, 347, 418
MlyI GAGTC 1 cut(s) 134
MmeI TCCRAC 2 cut(s) 234, 577
MnlI CCTC 2 cut(s) 22, 25
MseI TTAA 4 cut(s) 177, 288, 585, 618
MspR9I CCNGG 3 cut(s) 237, 304, 501
MunI CAATTG 1 cut(s) 347
Mva1269I GAATGC 1 cut(s) 598
MvaI CCWGG 3 cut(s) 237, 304, 501
MvnI CGCG 1 cut(s) 8
MwoI GCNNNNNNNGC 3 cut(s) 14, 163, 385
NdeII GATC 3 cut(s) 45, 151, 339
NlaIII CATG 5 cut(s) 58, 329, 466, 592, 625
NlaIV GGNNCC 3 cut(s) 373, 511, 537
NspI RCATGY 1 cut(s) 625
PagI TCATGA 2 cut(s) 54, 325
PciI ACATGT 1 cut(s) 621
PctI GAATGC 1 cut(s) 598
PfeI GAWTC 1 cut(s) 469
PfoI TCCNGGA 1 cut(s) 499
PkrI GCNGC 4 cut(s) 390, 414, 480, 531
PleI GAGTC 1 cut(s) 133
PpsI GAGTC 1 cut(s) 133
PscI ACATGT 1 cut(s) 621
PshBI ATTAAT 1 cut(s) 288
Psp6I CCWGG 3 cut(s) 235, 302, 499
PspGI CCWGG 3 cut(s) 235, 302, 499
PspN4I GGNNCC 3 cut(s) 373, 511, 537
PstI CTGCAG 1 cut(s) 531
RsaI GTAC 3 cut(s) 68, 92, 122
RsaNI GTAC 3 cut(s) 67, 91, 121
SaqAI TTAA 4 cut(s) 177, 288, 585, 618
SatI GCNGC 4 cut(s) 389, 413, 479, 530
Sau3AI GATC 3 cut(s) 45, 151, 339
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 3 cut(s) 237, 304, 501
SduI GDGCHC 3 cut(s) 195, 548, 574
SetI ASST 8 cut(s) 36, 168, 185, 198, 238, 373, 537, 562
SexAI ACCWGGT 1 cut(s) 235
SfcI CTRYAG 1 cut(s) 527
SmlI CTYRAG 2 cut(s) 158, 312
SmoI CTYRAG 2 cut(s) 158, 312
Sse9I AATT 5 cut(s) 111, 213, 280, 347, 418
SsiI CCGC 1 cut(s) 513
SspI AATATT 1 cut(s) 493
StyD4I CCNGG 3 cut(s) 235, 302, 499
TaqI TCGA 1 cut(s) 472
TasI AATT 5 cut(s) 111, 213, 280, 347, 418
TatI WGTACW 1 cut(s) 66
TfiI GAWTC 1 cut(s) 469
Tru1I TTAA 4 cut(s) 177, 288, 585, 618
Tru9I TTAA 4 cut(s) 177, 288, 585, 618
TscAI CASTG 3 cut(s) 522, 531, 574
TseI GCWGC 4 cut(s) 388, 412, 478, 529
TspDTI ATGAA 5 cut(s) 71, 104, 342, 482, 605
TspGWI ACGGA 1 cut(s) 364
TspRI CASTG 3 cut(s) 522, 531, 574
VspI ATTAAT 1 cut(s) 288
XapI RAATTY 4 cut(s) 111, 213, 280, 418
XceI RCATGY 1 cut(s) 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.