mediator of RNA polymerase II transcription subunit
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 1745 |
| AccB1I | GGYRCC | 1 cut(s) | 1814 |
| AccI | GTMKAC | 2 cut(s) | 1528, 1695 |
| AciI | CCGC | 3 cut(s) | 1263, 1836, 1861 |
| AclWI | GGATC | 3 cut(s) | 245, 706, 1695 |
| AcoI | YGGCCR | 1 cut(s) | 501 |
| AcsI | RAATTY | 3 cut(s) | 979, 1090, 1886 |
| AdeI | CACNNNGTG | 2 cut(s) | 1517, 1643 |
| AfaI | GTAC | 4 cut(s) | 77, 783, 1578, 1954 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 35, 38, 260 |
| AgsI | TTSAA | 7 cut(s) | 45, 337, 988, 1054, 1786, 1893, 1946 |
| AjnI | CCWGG | 2 cut(s) | 1045, 1968 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 778, 810, 1046, 1078 |
| Alw21I | GWGCWC | 1 cut(s) | 381 |
| Alw26I | GTCTC | 3 cut(s) | 323, 492, 1958 |
| AlwI | GGATC | 3 cut(s) | 245, 706, 1695 |
| AoxI | GGCC | 3 cut(s) | 26, 501, 990 |
| ApoI | RAATTY | 3 cut(s) | 979, 1090, 1886 |
| AseI | ATTAAT | 1 cut(s) | 1098 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 717, 983 |
| AspLEI | GCGC | 3 cut(s) | 1005, 1333, 1746 |
| AspS9I | GGNCC | 2 cut(s) | 635, 1554 |
| AsuHPI | GGTGA | 1 cut(s) | 1035 |
| AsuNHI | GCTAGC | 1 cut(s) | 1568 |
| AvaII | GGWCC | 2 cut(s) | 635, 1554 |
| BaeGI | GKGCMC | 1 cut(s) | 266 |
| BalI | TGGCCA | 1 cut(s) | 503 |
| BanI | GGYRCC | 1 cut(s) | 1814 |
| BbsI | GAAGAC | 1 cut(s) | 1305 |
| Bbv12I | GWGCWC | 1 cut(s) | 381 |
| BccI | CCATC | 6 cut(s) | 107, 143, 179, 239, 1162, 1768 |
| BceAI | ACGGC | 1 cut(s) | 1802 |
| BciT130I | CCWGG | 2 cut(s) | 1047, 1970 |
| BclI | TGATCA | 2 cut(s) | 565, 733 |
| BcoDI | GTCTC | 3 cut(s) | 323, 492, 1958 |
| BfmI | CTRYAG | 2 cut(s) | 926, 1532 |
| BfoI | RGCGCY | 1 cut(s) | 1006 |
| Bme1390I | CCNGG | 2 cut(s) | 1047, 1970 |
| Bme18I | GGWCC | 2 cut(s) | 635, 1554 |
| BmgT120I | GGNCC | 2 cut(s) | 635, 1554 |
| BmiI | GGNNCC | 4 cut(s) | 552, 636, 720, 1816 |
| BmrFI | CCNGG | 2 cut(s) | 1047, 1970 |
| BmsI | GCATC | 7 cut(s) | 352, 456, 1144, 1224, 1393, 1689, 1755 |
| BmtI | GCTAGC | 1 cut(s) | 1572 |
| BpiI | GAAGAC | 1 cut(s) | 1305 |
| BpmI | CTGGAG | 2 cut(s) | 261, 774 |
| BpuEI | CTTGAG | 2 cut(s) | 18, 365 |
| BsaBI | GATNNNNATC | 2 cut(s) | 249, 1679 |
| BsaI | GGTCTC | 1 cut(s) | 1958 |
| BsaJI | CCNNGG | 2 cut(s) | 23, 1968 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 470, 500 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 35, 38, 260 |
| Bse1I | ACTGG | 3 cut(s) | 433, 836, 1121 |
| Bse3DI | GCAATG | 2 cut(s) | 1268, 1430 |
| Bse8I | GATNNNNATC | 2 cut(s) | 249, 1679 |
| BseBI | CCWGG | 2 cut(s) | 1047, 1970 |
| BseDI | CCNNGG | 2 cut(s) | 23, 1968 |
| BseGI | GGATG | 2 cut(s) | 1384, 1746 |
| BseJI | GATNNNNATC | 2 cut(s) | 249, 1679 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 35, 38, 260 |
| BseMI | GCAATG | 2 cut(s) | 1268, 1430 |
| BseMII | CTCAG | 2 cut(s) | 503, 534 |
| BseNI | ACTGG | 3 cut(s) | 433, 836, 1121 |
| BseRI | GAGGAG | 3 cut(s) | 21, 1741, 1744 |
| BseSI | GKGCMC | 1 cut(s) | 266 |
| BshFI | GGCC | 3 cut(s) | 28, 503, 992 |
| BshNI | GGYRCC | 1 cut(s) | 1814 |
| BsiHKAI | GWGCWC | 1 cut(s) | 381 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 35, 38, 260 |
| BsmAI | GTCTC | 3 cut(s) | 323, 492, 1958 |
| BsmI | GAATGC | 1 cut(s) | 1504 |
| BsnI | GGCC | 3 cut(s) | 28, 503, 992 |
| Bso31I | GGTCTC | 1 cut(s) | 1958 |
| Bsp143I | GATC | 8 cut(s) | 250, 480, 565, 698, 733, 855, 1674, 1687 |
| BspACI | CCGC | 3 cut(s) | 1263, 1836, 1861 |
| BspANI | GGCC | 3 cut(s) | 28, 503, 992 |
| BspCNI | CTCAG | 2 cut(s) | 502, 535 |
| BspHI | TCATGA | 2 cut(s) | 864, 1210 |
| BspLI | GGNNCC | 4 cut(s) | 552, 636, 720, 1816 |
| BspMAI | CTGCAG | 2 cut(s) | 930, 1536 |
| BspOI | GCTAGC | 1 cut(s) | 1572 |
| BspPI | GGATC | 3 cut(s) | 245, 706, 1695 |
| BspT107I | GGYRCC | 1 cut(s) | 1814 |
| BspTNI | GGTCTC | 1 cut(s) | 1958 |
| BsrDI | GCAATG | 2 cut(s) | 1268, 1430 |
| BsrI | ACTGG | 3 cut(s) | 433, 836, 1121 |
| BssECI | CCNNGG | 2 cut(s) | 23, 1968 |
| BssMI | GATC | 8 cut(s) | 250, 480, 565, 698, 733, 855, 1674, 1687 |
| BssNAI | GTATAC | 1 cut(s) | 1529 |
| Bst1107I | GTATAC | 1 cut(s) | 1529 |
| Bst2UI | CCWGG | 2 cut(s) | 1047, 1970 |
| Bst4CI | ACNGT | 3 cut(s) | 516, 1608, 1830 |
| Bst6I | CTCTTC | 3 cut(s) | 332, 834, 945 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1171 |
| BstC8I | GCNNGC | 2 cut(s) | 1570, 1927 |
| BstDEI | CTNAG | 5 cut(s) | 489, 543, 890, 1789, 1903 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 33 |
| BstF5I | GGATG | 2 cut(s) | 1384, 1746 |
| BstH2I | RGCGCY | 1 cut(s) | 1006 |
| BstHHI | GCGC | 3 cut(s) | 1005, 1333, 1746 |
| BstKTI | GATC | 8 cut(s) | 253, 483, 568, 701, 736, 858, 1677, 1690 |
| BstMAI | GTCTC | 3 cut(s) | 323, 492, 1958 |
| BstMBI | GATC | 8 cut(s) | 250, 480, 565, 698, 733, 855, 1674, 1687 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 1141, 1171, 1664 |
| BstNI | CCWGG | 2 cut(s) | 1047, 1970 |
| BstNSI | RCATGY | 1 cut(s) | 818 |
| BstSCI | CCNGG | 2 cut(s) | 1045, 1968 |
| BstSFI | CTRYAG | 2 cut(s) | 926, 1532 |
| BstSLI | GKGCMC | 1 cut(s) | 266 |
| BstV2I | GAAGAC | 1 cut(s) | 1305 |
| BstX2I | RGATCY | 2 cut(s) | 250, 1687 |
| BstYI | RGATCY | 2 cut(s) | 250, 1687 |
| BstZ17I | GTATAC | 1 cut(s) | 1529 |
| BsuRI | GGCC | 3 cut(s) | 28, 503, 992 |
| BtsCI | GGATG | 2 cut(s) | 1384, 1746 |
| BtsIMutI | CAGTG | 2 cut(s) | 1421, 1480 |
| Cac8I | GCNNGC | 2 cut(s) | 1570, 1927 |
| CciI | TCATGA | 2 cut(s) | 864, 1210 |
| CfoI | GCGC | 3 cut(s) | 1005, 1333, 1746 |
| Cfr13I | GGNCC | 2 cut(s) | 635, 1554 |
| CsiI | ACCWGGT | 1 cut(s) | 1045 |
| Csp6I | GTAC | 4 cut(s) | 76, 782, 1577, 1953 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 712, 747 |
| CviAII | CATG | 8 cut(s) | 815, 865, 994, 1171, 1211, 1309, 1342, 1856 |
| CviQI | GTAC | 4 cut(s) | 76, 782, 1577, 1953 |
| DdeI | CTNAG | 5 cut(s) | 489, 543, 890, 1789, 1903 |
| DpnI | GATC | 8 cut(s) | 252, 482, 567, 700, 735, 857, 1676, 1689 |
| DpnII | GATC | 8 cut(s) | 250, 480, 565, 698, 733, 855, 1674, 1687 |
| DraIII | CACNNNGTG | 2 cut(s) | 1517, 1643 |
| EaeI | YGGCCR | 1 cut(s) | 501 |
| Eam1104I | CTCTTC | 3 cut(s) | 332, 834, 945 |
| EarI | CTCTTC | 3 cut(s) | 332, 834, 945 |
| Ecl136II | GAGCTC | 1 cut(s) | 379 |
| Eco31I | GGTCTC | 1 cut(s) | 1958 |
| Eco47I | GGWCC | 2 cut(s) | 635, 1554 |
| Eco53kI | GAGCTC | 1 cut(s) | 379 |
| EcoICRI | GAGCTC | 1 cut(s) | 379 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 33 |
| EcoRII | CCWGG | 2 cut(s) | 1045, 1968 |
| FaeI | CATG | 8 cut(s) | 818, 868, 997, 1174, 1214, 1312, 1345, 1859 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 687, 719 |
| FatI | CATG | 8 cut(s) | 814, 864, 993, 1170, 1210, 1308, 1341, 1855 |
| FbaI | TGATCA | 2 cut(s) | 565, 733 |
| FblI | GTMKAC | 2 cut(s) | 1528, 1695 |
| FokI | GGATG | 2 cut(s) | 1371, 1733 |
| FspI | TGCGCA | 1 cut(s) | 1745 |
| GlaI | GCGC | 3 cut(s) | 1004, 1332, 1745 |
| GsuI | CTGGAG | 2 cut(s) | 261, 774 |
| HaeII | RGCGCY | 1 cut(s) | 1006 |
| HaeIII | GGCC | 3 cut(s) | 28, 503, 992 |
| HhaI | GCGC | 3 cut(s) | 1005, 1333, 1746 |
| Hin1II | CATG | 8 cut(s) | 818, 868, 997, 1174, 1214, 1312, 1345, 1859 |
| Hin6I | GCGC | 3 cut(s) | 1003, 1331, 1744 |
| HinP1I | GCGC | 3 cut(s) | 1003, 1331, 1744 |
| HincII | GTYRAC | 2 cut(s) | 316, 829 |
| HindII | GTYRAC | 2 cut(s) | 316, 829 |
| HinfI | GANTC | 4 cut(s) | 280, 530, 794, 1403 |
| HphI | GGTGA | 1 cut(s) | 1035 |
| Hpy166II | GTNNAC | 8 cut(s) | 316, 371, 829, 1529, 1554, 1604, 1696, 1953 |
| Hpy8I | GTNNAC | 8 cut(s) | 316, 371, 829, 1529, 1554, 1604, 1696, 1953 |
| HpyAV | CCTTC | 9 cut(s) | 108, 144, 216, 246, 572, 656, 689, 862, 1760 |
| HpyCH4III | ACNGT | 3 cut(s) | 516, 1608, 1830 |
| HpyCH4IV | ACGT | 2 cut(s) | 1111, 1494 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 1141, 1171, 1664 |
| HpyF3I | CTNAG | 5 cut(s) | 489, 543, 890, 1789, 1903 |
| HpySE526I | ACGT | 2 cut(s) | 1111, 1494 |
| Hsp92II | CATG | 8 cut(s) | 818, 868, 997, 1174, 1214, 1312, 1345, 1859 |
| HspAI | GCGC | 3 cut(s) | 1003, 1331, 1744 |
| Ksp22I | TGATCA | 2 cut(s) | 565, 733 |
| Kzo9I | GATC | 8 cut(s) | 250, 480, 565, 698, 733, 855, 1674, 1687 |
| LmnI | GCTCC | 3 cut(s) | 376, 1000, 1152 |
| LweI | GCATC | 7 cut(s) | 352, 456, 1144, 1224, 1393, 1689, 1755 |
| MabI | ACCWGGT | 1 cut(s) | 1045 |
| MaeII | ACGT | 2 cut(s) | 1111, 1494 |
| MaeIII | GTNAC | 2 cut(s) | 1391, 1769 |
| MalI | GATC | 8 cut(s) | 252, 482, 567, 700, 735, 857, 1676, 1689 |
| MboI | GATC | 8 cut(s) | 250, 480, 565, 698, 733, 855, 1674, 1687 |
| MfeI | CAATTG | 1 cut(s) | 284 |
| MflI | RGATCY | 2 cut(s) | 250, 1687 |
| MlsI | TGGCCA | 1 cut(s) | 503 |
| MluNI | TGGCCA | 1 cut(s) | 503 |
| MmeI | TCCRAC | 2 cut(s) | 417, 978 |
| Mox20I | TGGCCA | 1 cut(s) | 503 |
| MroXI | GAANNNNTTC | 2 cut(s) | 717, 983 |
| MscI | TGGCCA | 1 cut(s) | 503 |
| MseI | TTAA | 5 cut(s) | 227, 1098, 1241, 1562, 1984 |
| MslI | CAYNNNNRTG | 3 cut(s) | 819, 1549, 1846 |
| Msp20I | TGGCCA | 1 cut(s) | 503 |
| MspA1I | CMGCKG | 1 cut(s) | 1595 |
| MspR9I | CCNGG | 2 cut(s) | 1047, 1970 |
| MunI | CAATTG | 1 cut(s) | 284 |
| Mva1269I | GAATGC | 1 cut(s) | 1504 |
| MvaI | CCWGG | 2 cut(s) | 1047, 1970 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 1141, 1171, 1664 |
| NdeII | GATC | 8 cut(s) | 250, 480, 565, 698, 733, 855, 1674, 1687 |
| NheI | GCTAGC | 1 cut(s) | 1568 |
| NlaIII | CATG | 8 cut(s) | 818, 868, 997, 1174, 1214, 1312, 1345, 1859 |
| NlaIV | GGNNCC | 4 cut(s) | 552, 636, 720, 1816 |
| NmeAIII | GCCGAG | 1 cut(s) | 4 |
| NsbI | TGCGCA | 1 cut(s) | 1745 |
| NspI | RCATGY | 1 cut(s) | 818 |
| PagI | TCATGA | 2 cut(s) | 864, 1210 |
| PctI | GAATGC | 1 cut(s) | 1504 |
| PdmI | GAANNNNTTC | 2 cut(s) | 717, 983 |
| PfeI | GAWTC | 4 cut(s) | 280, 530, 794, 1403 |
| PshBI | ATTAAT | 1 cut(s) | 1098 |
| Psp124BI | GAGCTC | 1 cut(s) | 381 |
| Psp6I | CCWGG | 2 cut(s) | 1045, 1968 |
| PspGI | CCWGG | 2 cut(s) | 1045, 1968 |
| PspN4I | GGNNCC | 4 cut(s) | 552, 636, 720, 1816 |
| PspPI | GGNCC | 2 cut(s) | 635, 1554 |
| PstI | CTGCAG | 2 cut(s) | 930, 1536 |
| PsuI | RGATCY | 2 cut(s) | 250, 1687 |
| PvuII | CAGCTG | 1 cut(s) | 1595 |
| RsaI | GTAC | 4 cut(s) | 77, 783, 1578, 1954 |
| RsaNI | GTAC | 4 cut(s) | 76, 782, 1577, 1953 |
| RseI | CAYNNNNRTG | 3 cut(s) | 819, 1549, 1846 |
| SacI | GAGCTC | 1 cut(s) | 381 |
| SaqAI | TTAA | 5 cut(s) | 227, 1098, 1241, 1562, 1984 |
| Sau3AI | GATC | 8 cut(s) | 250, 480, 565, 698, 733, 855, 1674, 1687 |
| Sau96I | GGNCC | 2 cut(s) | 635, 1554 |
| ScrFI | CCNGG | 2 cut(s) | 1047, 1970 |
| SexAI | ACCWGGT | 1 cut(s) | 1045 |
| SfaNI | GCATC | 7 cut(s) | 352, 456, 1144, 1224, 1393, 1689, 1755 |
| SfcI | CTRYAG | 2 cut(s) | 926, 1532 |
| SinI | GGWCC | 2 cut(s) | 635, 1554 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 819, 1549, 1846 |
| SmlI | CTYRAG | 2 cut(s) | 33, 380 |
| SmoI | CTYRAG | 2 cut(s) | 33, 380 |
| SsiI | CCGC | 3 cut(s) | 1263, 1836, 1861 |
| SspI | AATATT | 3 cut(s) | 586, 754, 1540 |
| SstI | GAGCTC | 1 cut(s) | 381 |
| StyD4I | CCNGG | 2 cut(s) | 1045, 1968 |
| TaaI | ACNGT | 3 cut(s) | 516, 1608, 1830 |
| TaiI | ACGT | 2 cut(s) | 1114, 1497 |
| TaqI | TCGA | 2 cut(s) | 672, 858 |
| TaqII | GACCGA | 1 cut(s) | 606 |
| TatI | WGTACW | 1 cut(s) | 1576 |
| TauI | GCSGC | 1 cut(s) | 1265 |
| TfiI | GAWTC | 4 cut(s) | 280, 530, 794, 1403 |
| Tru1I | TTAA | 5 cut(s) | 227, 1098, 1241, 1562, 1984 |
| Tru9I | TTAA | 5 cut(s) | 227, 1098, 1241, 1562, 1984 |
| TscAI | CASTG | 2 cut(s) | 1428, 1480 |
| TspGWI | ACGGA | 1 cut(s) | 463 |
| TspRI | CASTG | 2 cut(s) | 1428, 1480 |
| VpaK11BI | GGWCC | 2 cut(s) | 635, 1554 |
| VspI | ATTAAT | 1 cut(s) | 1098 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 33 |
| XapI | RAATTY | 3 cut(s) | 979, 1090, 1886 |
| XbaI | TCTAGA | 1 cut(s) | 1931 |
| XceI | RCATGY | 1 cut(s) | 818 |
| XmiI | GTMKAC | 2 cut(s) | 1528, 1695 |
| XmnI | GAANNNNTTC | 2 cut(s) | 717, 983 |