MD03G1033000.v1.1

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001201
Physical Location & Seq
Reverse (-)
2651062 .. 2653545
2484 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1033000.v1.1.491

Sequence Viewer

Length: 1407 bp
ATGTACCATCAACAGATGCAACATTCGCTTTTGAAGCCAAAATATCAGCAGGGAAATATGCCACAACTTGTGCAACAGCAACAGCAGCAGAACCTTTTACAACCGACTCAGTTGCAGTCTTCCCAGCAATCTGTTATGCCAACATCATCCTTTATGCAGCTTTCGGTGCAATCATTTCTCTTGAGTCTTCAGCAGAATCAACAATCTGCTATGCAACAGTCATCACAACCCATGCTTCAGCAGCATTCACAGCCAGTTCTCCGGCAGCAACAGCAGCCTCAACAGGTAGCTGTTCATCAACAACAAACACCAATGGCACAACAGCCAATTTTACCCCAACAGCAGCAGCAGCCGCTGTGGAGGCCTTCTCAAGTTGGAGAGGCCGCCATGGATGCCGGCGATTGGAGGAGTCGGCTGCAGGCTGATTCAAGGCAAAGAATTGTCAACAAGATAATGGATACGTTGAAGAGGCATCTTCCCTTCTCCGGACAAGATGGATTACAGGAACTCAGTGAAATTGCTGTAAGGTTTAGAGGAAGAGATTTATACTGCAGCCACAAGCCAGGTTCTTCCATAATTCCGGAGTTCTTATTTCATATGTCGGATTATCTACGGGAAATTTCTCTGAAGATGCTCACAATGGAGACCAAGTCTCAGAATTCAATGGGCAATCCTTTACAATCTAACTCTGCTGCTTCTCTAGATTCCTCAGCTCAGACTGGATATGCAAGTGGGGGTGATTGGCAAGAGGAGGTCTATCAAAAAATCAAAGTCATGAAGGAAGGGTACTTACCCGAACTAAGTGAAATCACGGATGCTCTTCCACAACAACCAAAGTCAGAGAAGCTTGAGAAGCTAAAAATGTTCAAATCCATGTTAGAGCGCCTCATATCAGTCTTACAGATTTCCAAGAGTAGCATTTCACCTGGTTTGAAAGACAAGTTGGGTTTATATGAGAAGCAGATGGTAAATTTTAGAAATGCAAATAGACTGAGGAAGCAAGGTCGTCCTCTGCAACAAGGGCAGCTCCCCCCACCTCATATGCACTCTATGCAGCAGCCGCAGTCTCAAATTCGTCAAGTGCAGTCTCATGAAAATCAAATGAACCCGCAGTTGCAAACAATGAATTTACAAGGTTCTGTGCCAACAATGCAGCAGAACAATATGACAAGCTTGGAGCAAAATTCAATGTGTTCGATATCTGGGGTTTCAACAGCACAGCAGAACATGATGAATTCATTGCAGCTGAGTACAAATTTGGATTCAGGACAGGGAAATGCACTGAACTCTTTGCAGCAAGTTCCAGTGGGATCTATCCAACAAACTCCTGTCAGTGCTTCCCATCAAGCTAACATGAATGGTTTGTCATCGCAGAGTGGGGTTAGTATGCTTCAGCCAAATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

469

Amino Acids

52.82

Weight (kDa)

9.38

Isoelectric Point (pI)

73.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 133 - 177 1.6e-14 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 485, 580
AciI CCGC 4 cut(s) 353, 384, 1061, 1109
AclWI GGATC 1 cut(s) 1318
AcsI RAATTY 8 cut(s) 618, 658, 970, 1071, 1126, 1183, 1234, 1255
AcuI CTGAAG 4 cut(s) 173, 221, 647, 1376
AfaI GTAC 3 cut(s) 5, 788, 1252
AfiI CCNNNNNNNGG 2 cut(s) 402, 485
AgsI TTSAA 8 cut(s) 34, 429, 466, 663, 868, 934, 1188, 1212
AjnI CCWGG 2 cut(s) 562, 925
AjuI GAANNNNNNNTTGG 2 cut(s) 926, 958
AluBI AGCT 9 cut(s) 160, 290, 713, 847, 856, 1027, 1173, 1246, 1349
AluI AGCT 9 cut(s) 160, 290, 713, 847, 856, 1027, 1173, 1246, 1349
Alw26I GTCTC 4 cut(s) 638, 657, 1071, 1092
AlwI GGATC 1 cut(s) 1318
AlwNI CAGNNNCTG 1 cut(s) 355
Aor13HI TCCGGA 2 cut(s) 485, 580
AoxI GGCC 2 cut(s) 362, 381
ApoI RAATTY 8 cut(s) 618, 658, 970, 1071, 1126, 1183, 1234, 1255
AspLEI GCGC 1 cut(s) 885
AsuHPI GGTGA 2 cut(s) 749, 915
BarI GAAGNNNNNNTAC 4 cut(s) 770, 774, 802, 806
BbsI GAAGAC 2 cut(s) 111, 179
BbvCI CCTCAGC 1 cut(s) 709
BccI CCATC 4 cut(s) 15, 488, 958, 1350
BcgI CGANNNNNNTGC 2 cut(s) 94, 128
BciT130I CCWGG 2 cut(s) 564, 927
BciVI GTATCC 1 cut(s) 451
BcoDI GTCTC 4 cut(s) 638, 657, 1071, 1092
BfaI CTAG 1 cut(s) 701
BfmI CTRYAG 2 cut(s) 416, 550
BfoI RGCGCY 1 cut(s) 886
BfuI GTATCC 1 cut(s) 451
Bme1390I CCNGG 2 cut(s) 564, 927
BmrFI CCNGG 2 cut(s) 564, 927
BmsI GCATC 5 cut(s) 6, 382, 481, 621, 805
BpiI GAAGAC 2 cut(s) 111, 179
BplI GAGNNNNNCTC 2 cut(s) 352, 384
Bpu10I CCTNAGC 1 cut(s) 709
BpuEI CTTGAG 3 cut(s) 202, 354, 869
BsaI GGTCTC 1 cut(s) 638
BsaJI CCNNGG 1 cut(s) 387
BsaWI WCCGGW 2 cut(s) 485, 580
BsaXI ACNNNNNCTCC 2 cut(s) 635, 665
Bsc4I CCNNNNNNNGG 2 cut(s) 402, 485
Bse118I RCCGGY 1 cut(s) 395
Bse1I ACTGG 3 cut(s) 254, 724, 1304
Bse3DI GCAATG 1 cut(s) 1238
BseAI TCCGGA 2 cut(s) 485, 580
BseBI CCWGG 2 cut(s) 564, 927
BseDI CCNNGG 1 cut(s) 387
BseGI GGATG 3 cut(s) 146, 397, 820
BseLI CCNNNNNNNGG 2 cut(s) 402, 485
BseMI GCAATG 1 cut(s) 1238
BseMII CTCAG 7 cut(s) 122, 523, 668, 723, 728, 983, 1238
BseNI ACTGG 3 cut(s) 254, 724, 1304
BseRI GAGGAG 2 cut(s) 421, 764
BseYI CCCAGC 1 cut(s) 123
BsgI GTGCAG 1 cut(s) 1103
BshFI GGCC 2 cut(s) 364, 383
BsiSI CCGG 4 cut(s) 262, 396, 486, 581
BslI CCNNNNNNNGG 2 cut(s) 402, 485
BsmAI GTCTC 4 cut(s) 638, 657, 1071, 1092
BsmI GAATGC 1 cut(s) 244
BsnI GGCC 2 cut(s) 364, 383
Bso31I GGTCTC 1 cut(s) 638
Bsp13I TCCGGA 2 cut(s) 485, 580
Bsp143I GATC 1 cut(s) 1310
Bsp19I CCATGG 1 cut(s) 387
BspACI CCGC 4 cut(s) 353, 384, 1061, 1109
BspANI GGCC 2 cut(s) 364, 383
BspCNI CTCAG 7 cut(s) 121, 522, 667, 722, 727, 984, 1239
BspEI TCCGGA 2 cut(s) 485, 580
BspHI TCATGA 2 cut(s) 774, 1090
BspMAI CTGCAG 2 cut(s) 420, 554
BspPI GGATC 1 cut(s) 1318
BspQI GCTCTTC 1 cut(s) 825
BspTNI GGTCTC 1 cut(s) 638
BsrDI GCAATG 1 cut(s) 1238
BsrFI RCCGGY 1 cut(s) 395
BsrI ACTGG 3 cut(s) 254, 724, 1304
BssAI RCCGGY 1 cut(s) 395
BssECI CCNNGG 1 cut(s) 387
BssMI GATC 1 cut(s) 1310
BssT1I CCWWGG 1 cut(s) 387
Bst2UI CCWGG 2 cut(s) 564, 927
Bst4CI ACNGT 1 cut(s) 219
Bst6I CTCTTC 3 cut(s) 461, 532, 825
BstAPI GCANNNNNTGC 1 cut(s) 1051
BstC8I GCNNGC 2 cut(s) 397, 420
BstDEI CTNAG 8 cut(s) 108, 509, 654, 709, 714, 800, 992, 1247
BstDSI CCRYGG 1 cut(s) 387
BstF5I GGATG 3 cut(s) 146, 397, 820
BstH2I RGCGCY 1 cut(s) 886
BstHHI GCGC 1 cut(s) 885
BstKTI GATC 1 cut(s) 1313
BstMAI GTCTC 4 cut(s) 638, 657, 1071, 1092
BstMBI GATC 1 cut(s) 1310
BstNI CCWGG 2 cut(s) 564, 927
BstSCI CCNGG 2 cut(s) 562, 925
BstSFI CTRYAG 2 cut(s) 416, 550
BstV2I GAAGAC 2 cut(s) 111, 179
BstX2I RGATCY 1 cut(s) 1310
BstYI RGATCY 1 cut(s) 1310
BsuI GTATCC 1 cut(s) 451
BsuRI GGCC 2 cut(s) 364, 383
BtgI CCRYGG 1 cut(s) 387
BtgZI GCGATG 1 cut(s) 1353
BtsCI GGATG 3 cut(s) 146, 397, 820
BtsIMutI CAGTG 4 cut(s) 517, 1280, 1311, 1339
Cac8I GCNNGC 2 cut(s) 397, 420
CaiI CAGNNNCTG 1 cut(s) 355
CciI TCATGA 2 cut(s) 774, 1090
CfoI GCGC 1 cut(s) 885
Cfr10I RCCGGY 1 cut(s) 395
CsiI ACCWGGT 1 cut(s) 925
Csp6I GTAC 3 cut(s) 4, 787, 1251
CviAII CATG 7 cut(s) 232, 388, 775, 874, 1091, 1228, 1354
CviQI GTAC 3 cut(s) 4, 787, 1251
DdeI CTNAG 8 cut(s) 108, 509, 654, 709, 714, 800, 992, 1247
DpnI GATC 1 cut(s) 1312
DpnII GATC 1 cut(s) 1310
Eam1104I CTCTTC 3 cut(s) 461, 532, 825
EarI CTCTTC 3 cut(s) 461, 532, 825
Eco130I CCWWGG 1 cut(s) 387
Eco147I AGGCCT 1 cut(s) 364
Eco31I GGTCTC 1 cut(s) 638
Eco32I GATATC 1 cut(s) 1200
Eco57I CTGAAG 4 cut(s) 173, 221, 647, 1376
EcoRI GAATTC 2 cut(s) 658, 1234
EcoRII CCWGG 2 cut(s) 562, 925
EcoRV GATATC 1 cut(s) 1200
EcoT14I CCWWGG 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 7 cut(s) 235, 391, 778, 877, 1094, 1231, 1357
FatI CATG 7 cut(s) 231, 387, 774, 873, 1090, 1227, 1353
FauI CCCGC 1 cut(s) 1116
FauNDI CATATG 2 cut(s) 597, 1041
FokI GGATG 3 cut(s) 133, 404, 827
FspBI CTAG 1 cut(s) 701
GlaI GCGC 1 cut(s) 884
GsaI CCCAGC 1 cut(s) 127
HaeII RGCGCY 1 cut(s) 886
HaeIII GGCC 2 cut(s) 364, 383
HapII CCGG 4 cut(s) 262, 396, 486, 581
HhaI GCGC 1 cut(s) 885
Hin1II CATG 7 cut(s) 235, 391, 778, 877, 1094, 1231, 1357
Hin6I GCGC 1 cut(s) 883
HinP1I GCGC 1 cut(s) 883
HincII GTYRAC 1 cut(s) 445
HindII GTYRAC 1 cut(s) 445
HindIII AAGCTT 2 cut(s) 845, 1171
HinfI GANTC 7 cut(s) 106, 184, 196, 409, 425, 704, 1262
HpaII CCGG 4 cut(s) 262, 396, 486, 581
HphI GGTGA 2 cut(s) 749, 915
Hpy166II GTNNAC 1 cut(s) 445
Hpy188I TCNGA 5 cut(s) 604, 627, 657, 717, 841
Hpy188III TCNNGA 7 cut(s) 181, 486, 581, 701, 775, 1091, 1266
Hpy8I GTNNAC 1 cut(s) 445
HpyAV CCTTC 4 cut(s) 375, 490, 772, 776
HpyCH4III ACNGT 1 cut(s) 219
HpyCH4IV ACGT 1 cut(s) 461
HpyF3I CTNAG 8 cut(s) 108, 509, 654, 709, 714, 800, 992, 1247
HpySE526I ACGT 1 cut(s) 461
Hsp92II CATG 7 cut(s) 235, 391, 778, 877, 1094, 1231, 1357
HspAI GCGC 1 cut(s) 883
Kpn2I TCCGGA 2 cut(s) 485, 580
KroI GCCGGC 1 cut(s) 395
KroNI GCCGGC 1 cut(s) 397
Kzo9I GATC 1 cut(s) 1310
LguI GCTCTTC 1 cut(s) 825
LmnI GCTCC 2 cut(s) 1032, 1177
LweI GCATC 5 cut(s) 6, 382, 481, 621, 805
MabI ACCWGGT 1 cut(s) 925
MaeI CTAG 1 cut(s) 701
MaeII ACGT 1 cut(s) 461
MalI GATC 1 cut(s) 1312
MboI GATC 1 cut(s) 1310
MboII GAAGA 8 cut(s) 111, 179, 467, 478, 549, 561, 640, 812
MflI RGATCY 1 cut(s) 1310
MlyI GAGTC 3 cut(s) 100, 193, 418
MmeI TCCRAC 3 cut(s) 355, 582, 1342
MroI TCCGGA 2 cut(s) 485, 580
MroNI GCCGGC 1 cut(s) 395
MseI TTAA 1 cut(s) 1405
MspA1I CMGCKG 2 cut(s) 355, 1246
MspI CCGG 4 cut(s) 262, 396, 486, 581
MspR9I CCNGG 2 cut(s) 564, 927
Mva1269I GAATGC 1 cut(s) 244
MvaI CCWGG 2 cut(s) 564, 927
NaeI GCCGGC 1 cut(s) 397
NcoI CCATGG 1 cut(s) 387
NdeI CATATG 2 cut(s) 597, 1041
NdeII GATC 1 cut(s) 1310
NgoMIV GCCGGC 1 cut(s) 395
NlaIII CATG 7 cut(s) 235, 391, 778, 877, 1094, 1231, 1357
PagI TCATGA 2 cut(s) 774, 1090
PceI AGGCCT 1 cut(s) 364
PciSI GCTCTTC 1 cut(s) 825
PctI GAATGC 1 cut(s) 244
PdiI GCCGGC 1 cut(s) 397
PfeI GAWTC 4 cut(s) 196, 425, 704, 1262
PflFI GACNNNGTC 1 cut(s) 649
PleI GAGTC 3 cut(s) 100, 192, 417
PpsI GAGTC 3 cut(s) 100, 192, 417
Psp6I CCWGG 2 cut(s) 562, 925
PspFI CCCAGC 1 cut(s) 123
PspGI CCWGG 2 cut(s) 562, 925
PstI CTGCAG 2 cut(s) 420, 554
PstNI CAGNNNCTG 1 cut(s) 355
PsuI RGATCY 1 cut(s) 1310
PsyI GACNNNGTC 1 cut(s) 649
PvuII CAGCTG 1 cut(s) 1246
RsaI GTAC 3 cut(s) 5, 788, 1252
RsaNI GTAC 3 cut(s) 4, 787, 1251
SapI GCTCTTC 1 cut(s) 825
SaqAI TTAA 1 cut(s) 1405
Sau3AI GATC 1 cut(s) 1310
SchI GAGTC 3 cut(s) 100, 193, 418
ScrFI CCNGG 2 cut(s) 564, 927
SexAI ACCWGGT 1 cut(s) 925
SfaNI GCATC 5 cut(s) 6, 382, 481, 621, 805
SfcI CTRYAG 2 cut(s) 416, 550
SmlI CTYRAG 3 cut(s) 181, 369, 848
SmoI CTYRAG 3 cut(s) 181, 369, 848
SseBI AGGCCT 1 cut(s) 364
SsiI CCGC 4 cut(s) 353, 384, 1061, 1109
SspMI CTAG 1 cut(s) 701
StuI AGGCCT 1 cut(s) 364
StyD4I CCNGG 2 cut(s) 562, 925
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 1 cut(s) 219
TaiI ACGT 1 cut(s) 464
TaqI TCGA 1 cut(s) 1196
TatI WGTACW 1 cut(s) 1250
TauI GCSGC 3 cut(s) 355, 386, 1063
TfiI GAWTC 4 cut(s) 196, 425, 704, 1262
Tru1I TTAA 1 cut(s) 1405
Tru9I TTAA 1 cut(s) 1405
TscAI CASTG 4 cut(s) 517, 1287, 1311, 1339
TspDTI ATGAA 9 cut(s) 284, 584, 791, 1107, 1118, 1139, 1227, 1247, 1370
TspGWI ACGGA 1 cut(s) 827
TspRI CASTG 4 cut(s) 517, 1287, 1311, 1339
Tth111I GACNNNGTC 1 cut(s) 649
XapI RAATTY 8 cut(s) 618, 658, 970, 1071, 1126, 1183, 1234, 1255
XbaI TCTAGA 1 cut(s) 700
XspI CTAG 1 cut(s) 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.