Rh5BG521000

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001201
Physical Location & Seq
Forward (+)
82348982 .. 82351816
2835 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG521000.1

Sequence Viewer

Length: 717 bp
ATGCTAGTAGCTGCTGTTATAACATTGGAGGGTATGCAACTTCATCGGCAAAGGGAACTAGAGCAGTTTGTTGGGGAAGGTTTGGTTCCGATGGACGCGAATGGTGCTGATTGGCAAGAGGAGGTCTATCAAAAGATCAAAGTCATGAAGGAGATGTACTACTCCGAACTAAGTGAAATGTACCCAAAACTTGCTACTAAAATTCATCAGTACGAGTCCCTTCCACAACAGCCAATGTCAGATCGGCTTGAGAAGCTGAAGATGTTTAAGACCTTGCTGGAGCACCTGATTGCTGTCTTACAAATTTCCAAAACTAACATATCACCTGGTTTGAAGGAGAAGTTGGATTTATATGAGAAGCAGATGATAAATTTTATTAATGTAAATAGACCCAGGACTACTCAAGTGCAATCTCATGAAAATCAGATGATCCTACAATTGCAATCCCTGAACTTGCAAGGTTCCGTGCCAACAATGCAGCAGAACAATATGACGGGCTTGCAGCAAAATTCTATGTCTTCTTTATCTGGGGTTTCAACAGCACAGCAAAACATGATGAATCGATTGCAGCCAAGTTCCAATATTTATCCAGGACAAGGAACCGCACTGAACTCACTGCAGCAGGTTCCTGTGGGCTCTGTCCAACAAGCTTCTGTCAGTGCTCCCCAACAAGTTAACATGAATGCCTTACCATCACAGAATGGGATTAACATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

238

Amino Acids

26.82

Weight (kDa)

6.43

Isoelectric Point (pI)

50.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 20
Acc36I ACCTGC 1 cut(s) 613
AccII CGCG 1 cut(s) 98
AciI CCGC 1 cut(s) 603
AclWI GGATC 1 cut(s) 424
AcsI RAATTY 4 cut(s) 201, 303, 370, 508
AcuI CTGAAG 1 cut(s) 278
AfaI GTAC 3 cut(s) 158, 182, 212
AfiI CCNNNNNNNGG 1 cut(s) 596
AflIII ACRYGT 1 cut(s) 711
AgsI TTSAA 2 cut(s) 334, 537
AjnI CCWGG 3 cut(s) 325, 392, 589
AjuI GAANNNNNNNTTGG 2 cut(s) 326, 358
AluBI AGCT 3 cut(s) 11, 256, 650
AluI AGCT 3 cut(s) 11, 256, 650
Alw21I GWGCWC 2 cut(s) 285, 664
AlwI GGATC 1 cut(s) 424
ApeKI GCWGC 5 cut(s) 11, 478, 502, 568, 619
ApoI RAATTY 4 cut(s) 201, 303, 370, 508
AseI ATTAAT 1 cut(s) 378
AsuHPI GGTGA 1 cut(s) 315
BanII GRGCYC 1 cut(s) 638
BarI GAAGNNNNNNTAC 2 cut(s) 140, 172
BbsI GAAGAC 1 cut(s) 510
Bbv12I GWGCWC 2 cut(s) 285, 664
BbvI GCAGC 4 cut(s) 490, 514, 580, 631
BccI CCATC 2 cut(s) 85, 700
BcgI CGANNNNNNTGC 2 cut(s) 26, 60
BciT130I CCWGG 3 cut(s) 327, 394, 591
BfaI CTAG 2 cut(s) 5, 59
BfmI CTRYAG 1 cut(s) 617
BfuAI ACCTGC 1 cut(s) 613
BisI GCNGC 5 cut(s) 12, 479, 503, 569, 620
BlsI GCNGC 5 cut(s) 13, 480, 504, 570, 621
Bme1390I CCNGG 3 cut(s) 327, 394, 591
BmiI GGNNCC 4 cut(s) 87, 463, 601, 627
BmrFI CCNGG 3 cut(s) 327, 394, 591
BpiI GAAGAC 1 cut(s) 510
BpmI CTGGAG 1 cut(s) 299
BpuEI CTTGAG 2 cut(s) 269, 387
Bsa29I ATCGAT 1 cut(s) 562
BsaJI CCNNGG 1 cut(s) 392
Bsc4I CCNNNNNNNGG 1 cut(s) 596
BseBI CCWGG 3 cut(s) 327, 394, 591
BseCI ATCGAT 1 cut(s) 562
BseDI CCNNGG 1 cut(s) 392
BseLI CCNNNNNNNGG 1 cut(s) 596
BseRI GAGGAG 1 cut(s) 134
BseXI GCAGC 4 cut(s) 490, 514, 580, 631
Bsh1236I CGCG 1 cut(s) 98
BshVI ATCGAT 1 cut(s) 562
BsiHKAI GWGCWC 2 cut(s) 285, 664
BslFI GGGAC 1 cut(s) 202
BslI CCNNNNNNNGG 1 cut(s) 596
BsmFI GGGAC 1 cut(s) 202
BsmI GAATGC 1 cut(s) 688
Bsp1286I GDGCHC 3 cut(s) 285, 638, 664
Bsp143I GATC 3 cut(s) 135, 241, 429
BspACI CCGC 1 cut(s) 603
BspDI ATCGAT 1 cut(s) 562
BspFNI CGCG 1 cut(s) 98
BspHI TCATGA 2 cut(s) 144, 415
BspLI GGNNCC 4 cut(s) 87, 463, 601, 627
BspMAI CTGCAG 1 cut(s) 621
BspMI ACCTGC 1 cut(s) 613
BspPI GGATC 1 cut(s) 424
BssECI CCNNGG 1 cut(s) 392
BssMI GATC 3 cut(s) 135, 241, 429
Bst2UI CCWGG 3 cut(s) 327, 394, 591
BstC8I GCNNGC 1 cut(s) 500
BstDEI CTNAG 1 cut(s) 170
BstFNI CGCG 1 cut(s) 98
BstKTI GATC 3 cut(s) 138, 244, 432
BstMBI GATC 3 cut(s) 135, 241, 429
BstMWI GCNNNNNNNGC 3 cut(s) 104, 253, 475
BstNI CCWGG 3 cut(s) 327, 394, 591
BstNSI RCATGY 1 cut(s) 715
BstSCI CCNGG 3 cut(s) 325, 392, 589
BstSFI CTRYAG 1 cut(s) 617
BstUI CGCG 1 cut(s) 98
BstV1I GCAGC 4 cut(s) 490, 514, 580, 631
BstV2I GAAGAC 1 cut(s) 510
Bsu15I ATCGAT 1 cut(s) 562
BsuTUI ATCGAT 1 cut(s) 562
BtsI GCAGTG 1 cut(s) 614
BtsIMutI CAGTG 3 cut(s) 605, 614, 664
BveI ACCTGC 1 cut(s) 613
Cac8I GCNNGC 1 cut(s) 500
CciI TCATGA 2 cut(s) 144, 415
ClaI ATCGAT 1 cut(s) 562
CseI GACGC 1 cut(s) 104
CsiI ACCWGGT 1 cut(s) 325
Csp6I GTAC 3 cut(s) 157, 181, 211
CviAII CATG 5 cut(s) 145, 416, 553, 679, 712
CviJI RGCY 8 cut(s) 11, 232, 247, 256, 498, 571, 636, 650
CviKI_1 RGCY 8 cut(s) 11, 232, 247, 256, 498, 571, 636, 650
CviQI GTAC 3 cut(s) 157, 181, 211
DdeI CTNAG 1 cut(s) 170
DpnI GATC 3 cut(s) 137, 243, 431
DpnII GATC 3 cut(s) 135, 241, 429
Eco24I GRGCYC 1 cut(s) 638
Eco57I CTGAAG 1 cut(s) 278
EcoRII CCWGG 3 cut(s) 325, 392, 589
EcoT38I GRGCYC 1 cut(s) 638
FaeI CATG 5 cut(s) 148, 419, 556, 682, 715
FaqI GGGAC 1 cut(s) 202
FatI CATG 5 cut(s) 144, 415, 552, 678, 711
Fnu4HI GCNGC 5 cut(s) 12, 479, 503, 569, 620
FriOI GRGCYC 1 cut(s) 638
Fsp4HI GCNGC 5 cut(s) 12, 479, 503, 569, 620
FspBI CTAG 2 cut(s) 5, 59
GluI GCNGC 5 cut(s) 12, 479, 503, 569, 620
GsuI CTGGAG 1 cut(s) 299
HgaI GACGC 1 cut(s) 104
Hin1II CATG 5 cut(s) 148, 419, 556, 682, 715
HincII GTYRAC 1 cut(s) 676
HindII GTYRAC 1 cut(s) 676
HindIII AAGCTT 1 cut(s) 648
HinfI GANTC 2 cut(s) 215, 559
HpaI GTTAAC 1 cut(s) 676
HphI GGTGA 1 cut(s) 315
Hpy166II GTNNAC 1 cut(s) 676
Hpy188I TCNGA 4 cut(s) 90, 166, 241, 426
Hpy188III TCNNGA 2 cut(s) 145, 416
Hpy8I GTNNAC 1 cut(s) 676
HpyAV CCTTC 4 cut(s) 71, 142, 230, 328
HpyCH4V TGCA 8 cut(s) 37, 409, 442, 457, 478, 502, 568, 619
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 253, 475
HpyF3I CTNAG 1 cut(s) 170
Hsp92II CATG 5 cut(s) 148, 419, 556, 682, 715
KspAI GTTAAC 1 cut(s) 676
Kzo9I GATC 3 cut(s) 135, 241, 429
LmnI GCTCC 2 cut(s) 280, 667
Lsp1109I GCAGC 4 cut(s) 490, 514, 580, 631
MabI ACCWGGT 1 cut(s) 325
MaeI CTAG 2 cut(s) 5, 59
MalI GATC 3 cut(s) 137, 243, 431
MboI GATC 3 cut(s) 135, 241, 429
MboII GAAGA 2 cut(s) 271, 510
MfeI CAATTG 1 cut(s) 437
MhlI GDGCHC 3 cut(s) 285, 638, 664
MluCI AATT 5 cut(s) 201, 303, 370, 437, 508
MlyI GAGTC 1 cut(s) 224
MmeI TCCRAC 2 cut(s) 324, 667
MnlI CCTC 3 cut(s) 22, 112, 115
MseI TTAA 4 cut(s) 267, 378, 675, 708
MspR9I CCNGG 3 cut(s) 327, 394, 591
MunI CAATTG 1 cut(s) 437
Mva1269I GAATGC 1 cut(s) 688
MvaI CCWGG 3 cut(s) 327, 394, 591
MvnI CGCG 1 cut(s) 98
MwoI GCNNNNNNNGC 3 cut(s) 104, 253, 475
NdeII GATC 3 cut(s) 135, 241, 429
NlaIII CATG 5 cut(s) 148, 419, 556, 682, 715
NlaIV GGNNCC 4 cut(s) 87, 463, 601, 627
NspI RCATGY 1 cut(s) 715
PagI TCATGA 2 cut(s) 144, 415
PciI ACATGT 1 cut(s) 711
PctI GAATGC 1 cut(s) 688
PfeI GAWTC 1 cut(s) 559
PfoI TCCNGGA 1 cut(s) 589
PkrI GCNGC 5 cut(s) 13, 480, 504, 570, 621
PleI GAGTC 1 cut(s) 223
PpsI GAGTC 1 cut(s) 223
PscI ACATGT 1 cut(s) 711
PshBI ATTAAT 1 cut(s) 378
PsiI TTATAA 1 cut(s) 20
Psp6I CCWGG 3 cut(s) 325, 392, 589
PspGI CCWGG 3 cut(s) 325, 392, 589
PspN4I GGNNCC 4 cut(s) 87, 463, 601, 627
PstI CTGCAG 1 cut(s) 621
RsaI GTAC 3 cut(s) 158, 182, 212
RsaNI GTAC 3 cut(s) 157, 181, 211
SaqAI TTAA 4 cut(s) 267, 378, 675, 708
SatI GCNGC 5 cut(s) 12, 479, 503, 569, 620
Sau3AI GATC 3 cut(s) 135, 241, 429
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 3 cut(s) 327, 394, 591
SduI GDGCHC 3 cut(s) 285, 638, 664
SexAI ACCWGGT 1 cut(s) 325
SfcI CTRYAG 1 cut(s) 617
SmlI CTYRAG 2 cut(s) 248, 402
SmoI CTYRAG 2 cut(s) 248, 402
Sse9I AATT 5 cut(s) 201, 303, 370, 437, 508
SsiI CCGC 1 cut(s) 603
SspI AATATT 1 cut(s) 583
SspMI CTAG 2 cut(s) 5, 59
StyD4I CCNGG 3 cut(s) 325, 392, 589
TaqI TCGA 1 cut(s) 562
TasI AATT 5 cut(s) 201, 303, 370, 437, 508
TatI WGTACW 1 cut(s) 156
TfiI GAWTC 1 cut(s) 559
Tru1I TTAA 4 cut(s) 267, 378, 675, 708
Tru9I TTAA 4 cut(s) 267, 378, 675, 708
TscAI CASTG 3 cut(s) 612, 621, 664
TseI GCWGC 5 cut(s) 11, 478, 502, 568, 619
TspDTI ATGAA 6 cut(s) 32, 161, 194, 432, 572, 695
TspGWI ACGGA 1 cut(s) 454
TspRI CASTG 3 cut(s) 612, 621, 664
VspI ATTAAT 1 cut(s) 378
XapI RAATTY 4 cut(s) 201, 303, 370, 508
XceI RCATGY 1 cut(s) 715
XspI CTAG 2 cut(s) 5, 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.