FvH4_4g01380

Vesicle-fusing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0003337
Physical Location & Seq
Forward (+)
1270595 .. 1273111
2517 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g01380.t1

Sequence Viewer

Length: 843 bp
ATGGATGATTTCCTACGTGCACTCCATGAAATTGTACCTGCATTTGGTGCCTCCACTGATGACCTTGAAAGATGCAGACTTCATGGAATGGTTGATTGTGGGGAAGATCATAAGTATATCTATCAAAGAGCCATGCTGCTTGTGGAGCAAGTTAAATCAAGCGACAGAAGCCCCCTTGTTACATGCCTTTTGGAAGGTCCTAGTGGCAGTGGTAAATCTTCACTAGCAGCTACTGTTGGCATCGACAGTGATTTCCCCTATGTTAAAATTATCTCGGCTGAGGCAATGATTGGTCTTCACGAAAGCACTAAATGTGCGCAAATCGCAAAGGTTTTTGAAGATGCATACAAGTCGCCATTGAGCATTATTATCCTTGATAATATTGAAAGGTTACTAGAGTATATCGCTATTGGTCCAAGGTTTTTGAATCTGATATCTCAAACACTGATGCTCCTTCTCAAGCGCACTCCTCCAAAGGGGAAAAAGCTTCTGGTTTTTGGTACAACAAGTGAAATCTGCTTCTTAGACTCGATTGGGTTTTGTGATACCTTCTCCGTCACCTACAATGTTCCCACATTAAAGACAGAGGCTGCAAAGAAGGTGCTAGAACAGTTAAATGTCTTTGCTGATGAAGACATAGATGATGCTGCAGAGGCCCTGAATGATATTCCTATCAAGAAGCTCTATATGGTAATTGAGATGGCAGCCCAGGGTGAGCAAGGAGGATCTTCCCAGGCAATCTTCTCCGGCCAATTGAAAATAGAGATCAAACACTTTCGTGGAATCCTCAAGGATACTGTTCGAGAGTTCAAGAAAGAGTTCGATATTAGCTTCAATGTATAG

Protein Analysis

281

Amino Acids

31.07

Weight (kDa)

5.17

Isoelectric Point (pI)

34.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA PF00004 63 - 173 1.8e-08 ATPase family associated with various cellular activities (AAA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 318
Acc36I ACCTGC 1 cut(s) 46
AccB1I GGYRCC 1 cut(s) 47
AclWI GGATC 1 cut(s) 733
AcoI YGGCCR 1 cut(s) 748
AfaI GTAC 2 cut(s) 36, 502
AfiI CCNNNNNNNGG 2 cut(s) 44, 476
AgsI TTSAA 7 cut(s) 68, 338, 386, 427, 757, 811, 835
AjnI CCWGG 2 cut(s) 708, 732
AleI CACNNNNGTG 1 cut(s) 777
AluBI AGCT 4 cut(s) 230, 487, 682, 831
AluI AGCT 4 cut(s) 230, 487, 682, 831
Alw21I GWGCWC 1 cut(s) 22
Alw44I GTGCAC 1 cut(s) 18
AlwI GGATC 1 cut(s) 733
AlwNI CAGNNNCTG 2 cut(s) 233, 590
AoxI GGCC 2 cut(s) 654, 748
ApaLI GTGCAC 1 cut(s) 18
ApeKI GCWGC 5 cut(s) 136, 227, 590, 647, 704
Asp700I GAANNNNTTC 1 cut(s) 818
AspLEI GCGC 2 cut(s) 319, 465
AspS9I GGNCC 3 cut(s) 197, 413, 655
AsuHPI GGTGA 2 cut(s) 550, 725
AvaII GGWCC 2 cut(s) 197, 413
BaeGI GKGCMC 1 cut(s) 22
BanI GGYRCC 1 cut(s) 47
BbsI GAAGAC 2 cut(s) 287, 639
Bbv12I GWGCWC 1 cut(s) 22
BbvCI CCTCAGC 1 cut(s) 279
BbvI GCAGC 5 cut(s) 123, 239, 577, 634, 716
BccI CCATC 1 cut(s) 694
BcgI CGANNNNNNTGC 2 cut(s) 333, 367
BciT130I CCWGG 2 cut(s) 710, 734
BciVI GTATCC 1 cut(s) 787
BfaI CTAG 4 cut(s) 201, 224, 395, 605
BfmI CTRYAG 1 cut(s) 648
BfuAI ACCTGC 1 cut(s) 46
BfuI GTATCC 1 cut(s) 787
BisI GCNGC 5 cut(s) 137, 228, 591, 648, 705
BlsI GCNGC 5 cut(s) 138, 229, 592, 649, 706
Bme1390I CCNGG 2 cut(s) 710, 734
Bme18I GGWCC 2 cut(s) 197, 413
BmgT120I GGNCC 3 cut(s) 197, 413, 655
BmiI GGNNCC 1 cut(s) 49
BmrFI CCNGG 2 cut(s) 710, 734
BmsI GCATC 5 cut(s) 62, 249, 331, 438, 634
BpiI GAAGAC 2 cut(s) 287, 639
Bpu10I CCTNAGC 1 cut(s) 279
BpuEI CTTGAG 2 cut(s) 443, 773
BsaAI YACGTR 1 cut(s) 17
BsaJI CCNNGG 4 cut(s) 416, 708, 709, 732
BsaXI ACNNNNNCTCC 4 cut(s) 6, 36, 435, 465
Bsc4I CCNNNNNNNGG 2 cut(s) 44, 476
Bse3DI GCAATG 1 cut(s) 291
BseBI CCWGG 2 cut(s) 710, 734
BseDI CCNNGG 4 cut(s) 416, 708, 709, 732
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 2 cut(s) 44, 476
BseMI GCAATG 1 cut(s) 291
BseMII CTCAG 1 cut(s) 270
BseRI GAGGAG 1 cut(s) 459
BseSI GKGCMC 1 cut(s) 22
BseXI GCAGC 5 cut(s) 123, 239, 577, 634, 716
BshFI GGCC 2 cut(s) 656, 750
BshNI GGYRCC 1 cut(s) 47
BsiHKAI GWGCWC 1 cut(s) 22
BsiSI CCGG 1 cut(s) 747
BslI CCNNNNNNNGG 2 cut(s) 44, 476
BsnI GGCC 2 cut(s) 656, 750
Bsp1286I GDGCHC 1 cut(s) 22
Bsp143I GATC 3 cut(s) 106, 725, 765
BspANI GGCC 2 cut(s) 656, 750
BspCNI CTCAG 1 cut(s) 271
BspLI GGNNCC 1 cut(s) 49
BspMAI CTGCAG 1 cut(s) 652
BspMI ACCTGC 1 cut(s) 46
BspPI GGATC 1 cut(s) 733
BspT107I GGYRCC 1 cut(s) 47
BsrDI GCAATG 1 cut(s) 291
BssECI CCNNGG 4 cut(s) 416, 708, 709, 732
BssMI GATC 3 cut(s) 106, 725, 765
BssT1I CCWWGG 1 cut(s) 416
Bst2UI CCWGG 2 cut(s) 710, 734
Bst4CI ACNGT 4 cut(s) 235, 248, 612, 799
BstAPI GCANNNNNTGC 1 cut(s) 47
BstBAI YACGTR 1 cut(s) 17
BstDEI CTNAG 2 cut(s) 279, 523
BstF5I GGATG 1 cut(s) 10
BstHHI GCGC 2 cut(s) 319, 465
BstKTI GATC 3 cut(s) 109, 728, 768
BstMBI GATC 3 cut(s) 106, 725, 765
BstMWI GCNNNNNNNGC 5 cut(s) 47, 145, 168, 323, 653
BstNI CCWGG 2 cut(s) 710, 734
BstNSI RCATGY 1 cut(s) 186
BstSCI CCNGG 2 cut(s) 708, 732
BstSFI CTRYAG 1 cut(s) 648
BstSLI GKGCMC 1 cut(s) 22
BstV1I GCAGC 5 cut(s) 123, 239, 577, 634, 716
BstV2I GAAGAC 2 cut(s) 287, 639
BstX2I RGATCY 1 cut(s) 725
BstYI RGATCY 1 cut(s) 725
BsuI GTATCC 1 cut(s) 787
BsuRI GGCC 2 cut(s) 656, 750
BtsCI GGATG 1 cut(s) 10
BtsI GCAGTG 1 cut(s) 214
BtsIMutI CAGTG 4 cut(s) 54, 214, 253, 443
BveI ACCTGC 1 cut(s) 46
CaiI CAGNNNCTG 2 cut(s) 233, 590
CfoI GCGC 2 cut(s) 319, 465
Cfr13I GGNCC 3 cut(s) 197, 413, 655
Csp6I GTAC 2 cut(s) 35, 501
CviAII CATG 4 cut(s) 26, 83, 133, 183
CviQI GTAC 2 cut(s) 35, 501
DdeI CTNAG 2 cut(s) 279, 523
DpnI GATC 3 cut(s) 108, 727, 767
DpnII GATC 3 cut(s) 106, 725, 765
EaeI YGGCCR 1 cut(s) 748
Eco130I CCWWGG 1 cut(s) 416
Eco32I GATATC 1 cut(s) 435
Eco47I GGWCC 2 cut(s) 197, 413
EcoO109I RGGNCCY 2 cut(s) 197, 655
EcoRII CCWGG 2 cut(s) 708, 732
EcoRV GATATC 1 cut(s) 435
EcoT14I CCWWGG 1 cut(s) 416
EcoT22I ATGCAT 1 cut(s) 346
ErhI CCWWGG 1 cut(s) 416
FaeI CATG 4 cut(s) 29, 86, 136, 186
FatI CATG 4 cut(s) 25, 82, 132, 182
Fnu4HI GCNGC 5 cut(s) 137, 228, 591, 648, 705
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 5 cut(s) 137, 228, 591, 648, 705
FspBI CTAG 4 cut(s) 201, 224, 395, 605
FspI TGCGCA 1 cut(s) 318
GlaI GCGC 2 cut(s) 318, 464
GluI GCNGC 5 cut(s) 137, 228, 591, 648, 705
HaeIII GGCC 2 cut(s) 656, 750
HapII CCGG 1 cut(s) 747
HhaI GCGC 2 cut(s) 319, 465
Hin1II CATG 4 cut(s) 29, 86, 136, 186
Hin6I GCGC 2 cut(s) 317, 463
HinP1I GCGC 2 cut(s) 317, 463
HindIII AAGCTT 1 cut(s) 485
HinfI GANTC 3 cut(s) 427, 527, 783
HpaII CCGG 1 cut(s) 747
HphI GGTGA 2 cut(s) 550, 725
Hpy166II GTNNAC 1 cut(s) 20
Hpy188I TCNGA 1 cut(s) 432
Hpy188III TCNNGA 4 cut(s) 299, 676, 803, 811
Hpy8I GTNNAC 1 cut(s) 20
HpyAV CCTTC 4 cut(s) 188, 464, 559, 592
HpyCH4III ACNGT 4 cut(s) 235, 248, 612, 799
HpyCH4IV ACGT 1 cut(s) 16
HpyCH4V TGCA 6 cut(s) 20, 41, 75, 344, 593, 650
HpyF10VI GCNNNNNNNGC 5 cut(s) 47, 145, 168, 323, 653
HpyF3I CTNAG 2 cut(s) 279, 523
HpySE526I ACGT 1 cut(s) 16
Hsp92II CATG 4 cut(s) 29, 86, 136, 186
HspAI GCGC 2 cut(s) 317, 463
Kzo9I GATC 3 cut(s) 106, 725, 765
LmnI GCTCC 2 cut(s) 145, 456
LpnPI CCDG 8 cut(s) 51, 476, 671, 695, 719, 722, 746, 760
Lsp1109I GCAGC 5 cut(s) 123, 239, 577, 634, 716
LweI GCATC 5 cut(s) 62, 249, 331, 438, 634
MaeI CTAG 4 cut(s) 201, 224, 395, 605
MaeII ACGT 1 cut(s) 16
MaeIII GTNAC 3 cut(s) 178, 390, 556
MalI GATC 3 cut(s) 108, 727, 767
MboI GATC 3 cut(s) 106, 725, 765
MboII GAAGA 7 cut(s) 116, 210, 287, 350, 644, 720, 733
MfeI CAATTG 1 cut(s) 752
MflI RGATCY 1 cut(s) 725
MhlI GDGCHC 1 cut(s) 22
MluCI AATT 4 cut(s) 30, 267, 693, 752
MlyI GAGTC 1 cut(s) 521
MnlI CCTC 7 cut(s) 61, 274, 480, 580, 646, 716, 797
Mph1103I ATGCAT 1 cut(s) 346
MroXI GAANNNNTTC 1 cut(s) 818
MseI TTAA 4 cut(s) 153, 264, 578, 614
MslI CAYNNNNRTG 1 cut(s) 777
MspI CCGG 1 cut(s) 747
MspR9I CCNGG 2 cut(s) 710, 734
MunI CAATTG 1 cut(s) 752
MvaI CCWGG 2 cut(s) 710, 734
MwoI GCNNNNNNNGC 5 cut(s) 47, 145, 168, 323, 653
NdeII GATC 3 cut(s) 106, 725, 765
NlaIII CATG 4 cut(s) 29, 86, 136, 186
NlaIV GGNNCC 1 cut(s) 49
NmeAIII GCCGAG 1 cut(s) 254
NmuCI GTSAC 1 cut(s) 556
NsbI TGCGCA 1 cut(s) 318
NsiI ATGCAT 1 cut(s) 346
NspI RCATGY 1 cut(s) 186
OliI CACNNNNGTG 1 cut(s) 777
PasI CCCWGGG 1 cut(s) 709
PdmI GAANNNNTTC 1 cut(s) 818
PfeI GAWTC 2 cut(s) 427, 783
PkrI GCNGC 5 cut(s) 138, 229, 592, 649, 706
PleI GAGTC 1 cut(s) 521
PpsI GAGTC 1 cut(s) 521
Ppu21I YACGTR 1 cut(s) 17
PpuMI RGGWCCY 1 cut(s) 197
Psp5II RGGWCCY 1 cut(s) 197
Psp6I CCWGG 2 cut(s) 708, 732
PspGI CCWGG 2 cut(s) 708, 732
PspN4I GGNNCC 1 cut(s) 49
PspPI GGNCC 3 cut(s) 197, 413, 655
PspPPI RGGWCCY 1 cut(s) 197
PstI CTGCAG 1 cut(s) 652
PstNI CAGNNNCTG 2 cut(s) 233, 590
PsuI RGATCY 1 cut(s) 725
RsaI GTAC 2 cut(s) 36, 502
RsaNI GTAC 2 cut(s) 35, 501
RseI CAYNNNNRTG 1 cut(s) 777
SaqAI TTAA 4 cut(s) 153, 264, 578, 614
SatI GCNGC 5 cut(s) 137, 228, 591, 648, 705
Sau3AI GATC 3 cut(s) 106, 725, 765
Sau96I GGNCC 3 cut(s) 197, 413, 655
SchI GAGTC 1 cut(s) 521
ScrFI CCNGG 2 cut(s) 710, 734
SduI GDGCHC 1 cut(s) 22
SfaNI GCATC 5 cut(s) 62, 249, 331, 438, 634
SfcI CTRYAG 1 cut(s) 648
SinI GGWCC 2 cut(s) 197, 413
SmiMI CAYNNNNRTG 1 cut(s) 777
SmlI CTYRAG 2 cut(s) 458, 788
SmoI CTYRAG 2 cut(s) 458, 788
Sse9I AATT 4 cut(s) 30, 267, 693, 752
SspI AATATT 1 cut(s) 382
SspMI CTAG 4 cut(s) 201, 224, 395, 605
StyD4I CCNGG 2 cut(s) 708, 732
StyI CCWWGG 1 cut(s) 416
TaaI ACNGT 4 cut(s) 235, 248, 612, 799
TaiI ACGT 1 cut(s) 19
TaqI TCGA 4 cut(s) 243, 530, 802, 822
TasI AATT 4 cut(s) 30, 267, 693, 752
TfiI GAWTC 2 cut(s) 427, 783
Tru1I TTAA 4 cut(s) 153, 264, 578, 614
Tru9I TTAA 4 cut(s) 153, 264, 578, 614
TscAI CASTG 4 cut(s) 61, 214, 253, 450
TseFI GTSAC 1 cut(s) 556
TseI GCWGC 5 cut(s) 136, 227, 590, 647, 704
Tsp45I GTSAC 1 cut(s) 556
TspDTI ATGAA 3 cut(s) 42, 71, 645
TspGWI ACGGA 1 cut(s) 544
TspRI CASTG 4 cut(s) 61, 214, 253, 450
VneI GTGCAC 1 cut(s) 18
VpaK11BI GGWCC 2 cut(s) 197, 413
XceI RCATGY 1 cut(s) 186
XcmI CCANNNNNNNNNTGG 1 cut(s) 139
XmnI GAANNNNTTC 1 cut(s) 818
XspI CTAG 4 cut(s) 201, 224, 395, 605
Zsp2I ATGCAT 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.