Rh5CG071500

Vesicle-fusing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0003337
Physical Location & Seq
Forward (+)
5353415 .. 5357614
4200 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG071500.1

Sequence Viewer

Length: 1218 bp
ATGGAGCAAATTACAAAGGGAAAGAGGTTGATCTTGTTCCCACTTCCCCTTGAAGGCCATATAAACCCCATGCTACAGCTCGCTAATCTTCTACACTCCAAAGGCTTCTCCATAACCATCATCCACACCCAGTTCAACTCCCCAAACCCTTCACTCCATCCACACTTCACCTTTCGCTCAATCCCAGATGGGTTGTCTGAAACTGAGGCTTCCCCCAAAGATATCATCCTTCTCTTCTCTCTTTTCAATACTAAATGTGTTGAACCCTTCAAGTTATGCTTGTCTAGCTTGTTATCAGAGGAGCCTGTTGCTTGTTTGATCTCTGACTCTATATTCCATTTCACTCAATCTGTTGCTGAGAGCTTTAAGCTCCCAAGGATTGTCTTTGAGACCGGGGGTATGACTTCTTATGCTGTTTTTACTCAGTTTCCACTATTAAGAGAAAAGGGCTACCTTCCGGTCACGCAAGATTCCCAACTAGAAAAGGTGGTGACGGAGCTTCCACCTCTCAGAATCAAAGACCTTCCGAGCATACAGAAGGGTTACATTGCGCTGAATGCGATTCAACGACGGCATGCTAGGGTTTCTAGCGGTGACTCATTATCTGTGAGCAGGTTTACTCCGCCTGATGATTTCAACCTGACCGTGCTCACTCTGGAATTGGAGTTTGTGAAAAAAGGGACTAAGAGTGAACAGATTGATGCTGTTGCCTTGTCGAGCCAACTTAAAAAGAGGTTTATCAACCAGGTTATGACAGCCGGACAAAGAGTATCATTTGAGTACCTTGGGACTAATTACATTTTTACAGTCAATCAAGCTGTAAGATCCGAAGAGTCTAGCTCTGTTGAAAGAGGGATGATCTCAAGTGAAACATACTTCATTTTTGAGGCACCGAGTGGAAATGGAATAAAGATTGTGAACCAACGTTCTGCTACGACTAGCAATATCTTTAAGCACAAGGAATTCAACCTTCAATCACTTGGCATTGGTGGCCTAGGTGCTGAGTTTGCAGATATATTTCGAAGAGCTTTTGCCTCCCGTGTCTTCCCTGCACATGTTACAAATAAACTAGGGATTAAGCATGTGAAGGGTATGCTGCTTTATGGACCACCTGGTACTGGAAAAACTCTTATGGCTCGTCAAATTGGAAAAATGTTGAATGGGAAAGATCCAAAGGTTTGGAAGTGGATTCACCATTATTTTTCTTTGATTTGCTAA

Protein Analysis

405

Amino Acids

45.3

Weight (kDa)

9.41

Isoelectric Point (pI)

43.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC48_2 PF02933 232 - 276 5.2e-07 Cell division protein 48 (CDC48), domain 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 603
AccB1I GGYRCC 1 cut(s) 889
AciI CCGC 2 cut(s) 591, 623
AclI AACGTT 1 cut(s) 925
AclWI GGATC 2 cut(s) 819, 1163
AcsI RAATTY 1 cut(s) 962
AdeI CACNNNGTG 1 cut(s) 896
AfaI GTAC 2 cut(s) 782, 1117
AfiI CCNNNNNNNGG 2 cut(s) 53, 1118
AflIII ACRYGT 1 cut(s) 1054
AjnI CCWGG 2 cut(s) 744, 1111
AluBI AGCT 8 cut(s) 79, 288, 363, 370, 499, 818, 840, 1028
AluI AGCT 8 cut(s) 79, 288, 363, 370, 499, 818, 840, 1028
Alw21I GWGCWC 1 cut(s) 651
Alw26I GTCTC 1 cut(s) 383
AlwI GGATC 2 cut(s) 819, 1163
AoxI GGCC 2 cut(s) 55, 991
ApeKI GCWGC 1 cut(s) 1096
ApoI RAATTY 1 cut(s) 962
AspA2I CCTAGG 1 cut(s) 994
AspLEI GCGC 1 cut(s) 553
AspS9I GGNCC 1 cut(s) 1106
AsuC2I CCSGG 1 cut(s) 394
AsuHPI GGTGA 4 cut(s) 160, 502, 605, 1184
AsuII TTCGAA 1 cut(s) 1021
AvaII GGWCC 1 cut(s) 1106
AvrII CCTAGG 1 cut(s) 994
BanI GGYRCC 1 cut(s) 889
BbsI GAAGAC 1 cut(s) 1036
Bbv12I GWGCWC 1 cut(s) 651
BbvI GCAGC 1 cut(s) 1083
BccI CCATC 3 cut(s) 125, 165, 182
BceAI ACGGC 1 cut(s) 587
BciT130I CCWGG 2 cut(s) 746, 1113
BcnI CCSGG 1 cut(s) 394
BcoDI GTCTC 1 cut(s) 383
BfaI CTAG 8 cut(s) 285, 479, 579, 588, 837, 939, 995, 1070
BfmI CTRYAG 1 cut(s) 74
BfuAI ACCTGC 1 cut(s) 603
BisI GCNGC 1 cut(s) 1097
BlnI CCTAGG 1 cut(s) 994
BlsI GCNGC 1 cut(s) 1098
Bme1390I CCNGG 3 cut(s) 394, 746, 1113
Bme18I GGWCC 1 cut(s) 1106
BmgT120I GGNCC 1 cut(s) 1106
BmiI GGNNCC 2 cut(s) 303, 891
BmrFI CCNGG 3 cut(s) 394, 746, 1113
BmrI ACTGGG 1 cut(s) 124
BmsI GCATC 1 cut(s) 691
BmuI ACTGGG 1 cut(s) 124
BpiI GAAGAC 1 cut(s) 1036
BplI GAGNNNNNCTC 2 cut(s) 824, 856
Bpu14I TTCGAA 1 cut(s) 1021
BpuEI CTTGAG 1 cut(s) 847
BpuMI CCSGG 1 cut(s) 394
BsaI GGTCTC 1 cut(s) 383
BsaJI CCNNGG 4 cut(s) 374, 393, 784, 994
BsaWI WCCGGW 1 cut(s) 457
Bsc4I CCNNNNNNNGG 2 cut(s) 53, 1118
Bse1I ACTGG 2 cut(s) 130, 1123
Bse3DI GCAATG 1 cut(s) 546
BseBI CCWGG 2 cut(s) 746, 1113
BseDI CCNNGG 4 cut(s) 374, 393, 784, 994
BseGI GGATG 4 cut(s) 120, 157, 225, 861
BseLI CCNNNNNNNGG 2 cut(s) 53, 1118
BseMI GCAATG 1 cut(s) 546
BseMII CTCAG 5 cut(s) 195, 348, 437, 523, 993
BseNI ACTGG 2 cut(s) 130, 1123
BseRI GAGGAG 1 cut(s) 314
BseXI GCAGC 1 cut(s) 1083
BsgI GTGCAG 1 cut(s) 1035
BshFI GGCC 2 cut(s) 57, 993
BshNI GGYRCC 1 cut(s) 889
BsiHKAI GWGCWC 1 cut(s) 651
BsiSI CCGG 3 cut(s) 393, 458, 759
BslFI GGGAC 2 cut(s) 694, 802
BslI CCNNNNNNNGG 2 cut(s) 53, 1118
BsmAI GTCTC 1 cut(s) 383
BsmFI GGGAC 2 cut(s) 694, 802
BsmI GAATGC 1 cut(s) 562
BsnI GGCC 2 cut(s) 57, 993
Bso31I GGTCTC 1 cut(s) 383
Bsp119I TTCGAA 1 cut(s) 1021
Bsp1286I GDGCHC 1 cut(s) 651
Bsp143I GATC 5 cut(s) 30, 318, 824, 858, 1168
BspACI CCGC 2 cut(s) 591, 623
BspANI GGCC 2 cut(s) 57, 993
BspCNI CTCAG 5 cut(s) 196, 349, 436, 522, 994
BspLI GGNNCC 2 cut(s) 303, 891
BspMI ACCTGC 1 cut(s) 603
BspPI GGATC 2 cut(s) 819, 1163
BspQI GCTCTTC 1 cut(s) 1018
BspT104I TTCGAA 1 cut(s) 1021
BspT107I GGYRCC 1 cut(s) 889
BspTNI GGTCTC 1 cut(s) 383
BsrDI GCAATG 1 cut(s) 546
BsrI ACTGG 2 cut(s) 130, 1123
BssECI CCNNGG 4 cut(s) 374, 393, 784, 994
BssMI GATC 5 cut(s) 30, 318, 824, 858, 1168
BssT1I CCWWGG 3 cut(s) 374, 784, 994
Bst2UI CCWGG 2 cut(s) 746, 1113
Bst4CI ACNGT 2 cut(s) 646, 808
Bst6I CTCTTC 3 cut(s) 239, 825, 1018
BstBI TTCGAA 1 cut(s) 1021
BstC8I GCNNGC 2 cut(s) 81, 576
BstDEI CTNAG 6 cut(s) 204, 357, 423, 509, 684, 1002
BstF5I GGATG 4 cut(s) 120, 157, 225, 861
BstHHI GCGC 1 cut(s) 553
BstKTI GATC 5 cut(s) 33, 321, 827, 861, 1171
BstMAI GTCTC 1 cut(s) 383
BstMBI GATC 5 cut(s) 30, 318, 824, 858, 1168
BstMWI GCNNNNNNNGC 4 cut(s) 285, 557, 990, 1007
BstNI CCWGG 2 cut(s) 746, 1113
BstNSI RCATGY 3 cut(s) 578, 1058, 1085
BstSCI CCNGG 3 cut(s) 392, 744, 1111
BstSFI CTRYAG 1 cut(s) 74
BstV1I GCAGC 1 cut(s) 1083
BstV2I GAAGAC 1 cut(s) 1036
BstX2I RGATCY 2 cut(s) 824, 1168
BstXI CCANNNNNNTGG 1 cut(s) 1179
BstYI RGATCY 2 cut(s) 824, 1168
BsuRI GGCC 2 cut(s) 57, 993
BtsCI GGATG 4 cut(s) 120, 157, 225, 861
BveI ACCTGC 1 cut(s) 603
Cac8I GCNNGC 2 cut(s) 81, 576
CfoI GCGC 1 cut(s) 553
Cfr13I GGNCC 1 cut(s) 1106
CsiI ACCWGGT 2 cut(s) 744, 1111
Csp6I GTAC 2 cut(s) 781, 1116
CviAII CATG 4 cut(s) 70, 575, 1055, 1082
CviQI GTAC 2 cut(s) 781, 1116
DdeI CTNAG 6 cut(s) 204, 357, 423, 509, 684, 1002
DpnI GATC 5 cut(s) 32, 320, 826, 860, 1170
DpnII GATC 5 cut(s) 30, 318, 824, 858, 1168
DraIII CACNNNGTG 1 cut(s) 896
Eam1104I CTCTTC 3 cut(s) 239, 825, 1018
EarI CTCTTC 3 cut(s) 239, 825, 1018
EciI GGCGGA 1 cut(s) 612
Eco130I CCWWGG 3 cut(s) 374, 784, 994
Eco31I GGTCTC 1 cut(s) 383
Eco32I GATATC 1 cut(s) 223
Eco47I GGWCC 1 cut(s) 1106
EcoRI GAATTC 1 cut(s) 962
EcoRII CCWGG 2 cut(s) 744, 1111
EcoRV GATATC 1 cut(s) 223
EcoT14I CCWWGG 3 cut(s) 374, 784, 994
ErhI CCWWGG 3 cut(s) 374, 784, 994
FaeI CATG 4 cut(s) 73, 578, 1058, 1085
FalI AAGNNNNNCTT 2 cut(s) 263, 295
FaqI GGGAC 2 cut(s) 694, 802
FatI CATG 4 cut(s) 69, 574, 1054, 1081
Fnu4HI GCNGC 1 cut(s) 1097
FokI GGATG 4 cut(s) 107, 144, 212, 868
Fsp4HI GCNGC 1 cut(s) 1097
FspBI CTAG 8 cut(s) 285, 479, 579, 588, 837, 939, 995, 1070
GlaI GCGC 1 cut(s) 552
GluI GCNGC 1 cut(s) 1097
HaeIII GGCC 2 cut(s) 57, 993
HapII CCGG 3 cut(s) 393, 458, 759
HhaI GCGC 1 cut(s) 553
Hin1II CATG 4 cut(s) 73, 578, 1058, 1085
Hin6I GCGC 1 cut(s) 551
HinP1I GCGC 1 cut(s) 551
HinfI GANTC 7 cut(s) 326, 470, 513, 562, 596, 833, 1189
HpaII CCGG 3 cut(s) 393, 458, 759
HphI GGTGA 4 cut(s) 160, 502, 605, 1184
Hpy166II GTNNAC 3 cut(s) 618, 692, 919
Hpy188I TCNGA 6 cut(s) 199, 298, 325, 512, 528, 829
Hpy188III TCNNGA 1 cut(s) 656
Hpy8I GTNNAC 3 cut(s) 618, 692, 919
Hpy99I CGWCG 1 cut(s) 573
HpyAV CCTTC 9 cut(s) 47, 159, 239, 277, 464, 532, 533, 980, 1081
HpyCH4III ACNGT 2 cut(s) 646, 808
HpyCH4IV ACGT 1 cut(s) 925
HpyCH4V TGCA 2 cut(s) 1010, 1052
HpyF10VI GCNNNNNNNGC 4 cut(s) 285, 557, 990, 1007
HpyF3I CTNAG 6 cut(s) 204, 357, 423, 509, 684, 1002
HpySE526I ACGT 1 cut(s) 925
Hsp92II CATG 4 cut(s) 73, 578, 1058, 1085
HspAI GCGC 1 cut(s) 551
Kzo9I GATC 5 cut(s) 30, 318, 824, 858, 1168
LguI GCTCTTC 1 cut(s) 1018
LmnI GCTCC 4 cut(s) 4, 301, 375, 496
Lsp1109I GCAGC 1 cut(s) 1083
LweI GCATC 1 cut(s) 691
MabI ACCWGGT 2 cut(s) 744, 1111
MaeI CTAG 8 cut(s) 285, 479, 579, 588, 837, 939, 995, 1070
MaeII ACGT 1 cut(s) 925
MaeIII GTNAC 5 cut(s) 460, 490, 542, 593, 1057
MalI GATC 5 cut(s) 32, 320, 826, 860, 1170
MboI GATC 5 cut(s) 30, 318, 824, 858, 1168
MboII GAAGA 5 cut(s) 80, 226, 842, 1035, 1036
MflI RGATCY 2 cut(s) 824, 1168
MhlI GDGCHC 1 cut(s) 651
MluCI AATT 5 cut(s) 9, 659, 793, 962, 1143
MlyI GAGTC 3 cut(s) 320, 590, 842
MnlI CCTC 8 cut(s) 18, 199, 292, 516, 726, 845, 880, 1045
MseI TTAA 5 cut(s) 366, 437, 726, 951, 1077
MspI CCGG 3 cut(s) 393, 458, 759
MspR9I CCNGG 3 cut(s) 394, 746, 1113
Mva1269I GAATGC 1 cut(s) 562
MvaI CCWGG 2 cut(s) 746, 1113
MwoI GCNNNNNNNGC 4 cut(s) 285, 557, 990, 1007
NciI CCSGG 1 cut(s) 394
NdeII GATC 5 cut(s) 30, 318, 824, 858, 1168
NlaIII CATG 4 cut(s) 73, 578, 1058, 1085
NlaIV GGNNCC 2 cut(s) 303, 891
NmuCI GTSAC 3 cut(s) 460, 490, 593
NspI RCATGY 3 cut(s) 578, 1058, 1085
NspV TTCGAA 1 cut(s) 1021
PaeI GCATGC 1 cut(s) 578
PciI ACATGT 1 cut(s) 1054
PciSI GCTCTTC 1 cut(s) 1018
PctI GAATGC 1 cut(s) 562
PfeI GAWTC 4 cut(s) 470, 513, 562, 1189
PkrI GCNGC 1 cut(s) 1098
PleI GAGTC 3 cut(s) 320, 590, 841
PpsI GAGTC 3 cut(s) 320, 590, 841
PscI ACATGT 1 cut(s) 1054
Psp1406I AACGTT 1 cut(s) 925
Psp6I CCWGG 2 cut(s) 744, 1111
PspGI CCWGG 2 cut(s) 744, 1111
PspN4I GGNNCC 2 cut(s) 303, 891
PspPI GGNCC 1 cut(s) 1106
PsuI RGATCY 2 cut(s) 824, 1168
RsaI GTAC 2 cut(s) 782, 1117
RsaNI GTAC 2 cut(s) 781, 1116
SapI GCTCTTC 1 cut(s) 1018
SaqAI TTAA 5 cut(s) 366, 437, 726, 951, 1077
SatI GCNGC 1 cut(s) 1097
Sau3AI GATC 5 cut(s) 30, 318, 824, 858, 1168
Sau96I GGNCC 1 cut(s) 1106
SchI GAGTC 3 cut(s) 320, 590, 842
ScrFI CCNGG 3 cut(s) 394, 746, 1113
SduI GDGCHC 1 cut(s) 651
SexAI ACCWGGT 2 cut(s) 744, 1111
SfaNI GCATC 1 cut(s) 691
SfcI CTRYAG 1 cut(s) 74
SfuI TTCGAA 1 cut(s) 1021
SinI GGWCC 1 cut(s) 1106
SmlI CTYRAG 1 cut(s) 862
SmoI CTYRAG 1 cut(s) 862
SphI GCATGC 1 cut(s) 578
Sse9I AATT 5 cut(s) 9, 659, 793, 962, 1143
SsiI CCGC 2 cut(s) 591, 623
SspMI CTAG 8 cut(s) 285, 479, 579, 588, 837, 939, 995, 1070
StyD4I CCNGG 3 cut(s) 392, 744, 1111
StyI CCWWGG 3 cut(s) 374, 784, 994
TaaI ACNGT 2 cut(s) 646, 808
TaiI ACGT 1 cut(s) 928
TaqI TCGA 2 cut(s) 716, 1021
TasI AATT 5 cut(s) 9, 659, 793, 962, 1143
TfiI GAWTC 4 cut(s) 470, 513, 562, 1189
Tru1I TTAA 5 cut(s) 366, 437, 726, 951, 1077
Tru9I TTAA 5 cut(s) 366, 437, 726, 951, 1077
TseFI GTSAC 3 cut(s) 460, 490, 593
TseI GCWGC 1 cut(s) 1096
Tsp45I GTSAC 3 cut(s) 460, 490, 593
TspDTI ATGAA 1 cut(s) 868
TspGWI ACGGA 1 cut(s) 509
VpaK11BI GGWCC 1 cut(s) 1106
XapI RAATTY 1 cut(s) 962
XceI RCATGY 3 cut(s) 578, 1058, 1085
XmaJI CCTAGG 1 cut(s) 994
XspI CTAG 8 cut(s) 285, 479, 579, 588, 837, 939, 995, 1070
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.