Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003337

Physical Location & Seq

Genomic Coordinates Reverse (-)

46047776 .. 46053508

Gene Structure 5733 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1339600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1818 bp

ATGACGGCTGGGCAAAGAGTATCATTTGAGTTTCAGGGGATCAACTACATTTTTACTGTCAATCAAGCTGCAGTAGAGGGACAAGAAAAGTCTAATAATGGTATTGAAAGAGGGATGATTGTGAATGATACATATTTCATCTTTGAAACATCAAATGCAAGTGGAATAAAGATTGTAAATCAGCGTGAAGCTGCAAGTAGCAACATTTTCAGGCACAAGGAGTTTAATCTTCAAACGCTTGGAATTGGTGGCTTAGGTGCTGAGTTTGCAGATATATTTCGAAGAGCTTTTGCATCCCGTGTATTCCCTCCTCATGTGACAAATAAATTAGGGATTAAGCATGTAAAGGGTATGCTGTTATATGGGCCACCTGGTACTGGAAAAACTCTCATGGCTCGTCAAATTGGAAAGATGTTGAATGGGAAAGAACCAAAGATTGTTAATGGCCCTGAAGTTTTGAGCAAGTTCGTTGGTGAAACTGAGAAGAATGTTAGGGATCTATTTGCTGATGCTGAGAATGATCAAAGGGCTCGCGGAGATCAGAGTGACTTGCATGTGATAATCTTTGATGAAATTGATGCTATTTGTAAGTCAAGAGGATCAACTCGAGATGGTACTGGGGTTCATGACAGTATTGTGAACCAGTTACTCACGAAGATTGATGGTGTGGAGTCTCTTAATAATGTTTTGCTTATTGGGATGACAAACAGAAAGGATTTGCTTGATGAAGCTCTTTTGAGGCCAGGACGTCTGGAAGTTCAAATTGAGATAAGCCTTCCTGATGAAAATGGTCGTCTACAGATTCTTCAAATTCATACAAACAAGATGAAGGAGAGCTCTTTTCTTGCTCCAGATGTGAACCTTCAAGAGCTTGCGGCTCGCACGAAAAACTACAGTGGTGCAGAACTTGAAGGTGTTGTTAAAAGTGCAGTGTCATTTGCTCTGAATAGACAACTAAGTTTGGATGATCTTACCAAGCCAGTGGATGAAGAGAGCATTAAGGTTACTATGGACGATTTTCTACATGCACTCCAAGAAATTGTACCTGCATTTGGAGCCTCCACTGATGACCTTCAAAGATGCAGACTTAATGGAATGGTGGATTGTGGTGATCGTCATAAGCACATATATCAAAGAGCTATATTATTAGTGGAGCAAGTCAAATTGAGCAAAGGAAGCCCACTTGTTACATGCCTTTTGGAAGGCCCTAGTGGCAGTGGTAAATCTGCACTAGCAGCTACCGTTGGCATTGACAGTGATTTTCCTTATGTCAAGATTGTTTCGGCTGAGACAATGATTGGTCTTCATGAGAGCACCAAATGCGCACAAATTGTGAAGGTTTTTGAGGATGCATACAAATCACCGTTGAGCATCATTATCCTTGATGATATTGAAAGGTTACTGGAGTATGTTGCTATTGGTCCAAGGTTTTCCAATTTGATATCTCAAACACTGTTGGTCCTACTTAAGCGTCTCCCTCCAAAGGGGAAAAAACTTCTGGTCATTGGAACAACAAGTGAATTGGGCTTCTTAGATTCAATCGGTTTTTGTGATACCTTCTCTGTCACTTACAATGTTCCCACACTAAAGACGTTGGATGCAAAGAAGGTGCTGTCACAATTGAATGTTTTTGCTGATGAAGACATCGATGCGGCTGCAGAGGCCCTGAACGATATGCCCATCAAGAAGCTCTACATGTTAATTGAGATGGCAGCTCAGGGCGACCAAGGAGGATCTGCCGAGGCAATCTACTCCGGAGAAGAGAAGATAAACATCACTCACTTTTACGATTGCCTTCAGGACATTGTTCGATATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005794 GO:0005886 GO:0005911 GO:0009506 GO:0012505 GO:0016020 GO:0030054 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04138 ko04721 ko04727 ko04962 map04138 map04721 map04727 map04962

Pfam Domains

Protein Families

AAA CDC48_2 CDC48_N

Protein Analysis

606

Amino Acids

66.54

Weight (kDa)

5.43

Isoelectric Point (pI)

32.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA	PF00004	118 - 258	1.5e-36	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	285 - 320	2.6e-10	AAA+ lid domain
AAA	PF00004	399 - 512	2.8e-10	ATPase family associated with various cellular activities (AAA)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003337)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04910
fragaria_vesca	FvH4_3g09180 FvH4_4g01371 FvH4_4g01380
malus_domestica	MD05G1312500.v1.1 MD05G1339600.v1.1 MD10G1294300.v1.1
prunus_persica	Prupe.4G047600_v2.0.a1
pyrus_communis	pycom05g29040 pycom10g24600
rosa_chinensis	RchiOBHm_Chr4g0419821 RchiOBHm_Chr5g0005931 RchiOBHm_Chr5g0005941 RchiOBHm_Chr5g0008441
rosa_laevigata	RLG00000031340 RLG00000031347 RLG00000031352 RLG00000031389 RLG00000031572
rosa_multiflora	Rmu_sc0000720.1_g000006 Rmu_sc0004326.1_g000001 Rmu_sc0006414.1_g000006
rosa_roxburghii	Rroxscaffold_1G00068050
rosa_rugosa	Rorug04G0435800
rosa_samantha	Rh4AG222000 Rh4BG224200 Rh4CG235100 Rh4DG221000 Rh5AG064700 Rh5BG061700 Rh5CG071500 Rh5CG071600 Rh5CG071800 Rh5CG071900
rosa_wichuraiana	Rw4G019100 Rw5G005630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	751
Acc16I	TGCGCA	1 cut(s)	1324
Acc36I	ACCTGC	1 cut(s)	1054
AccI	GTMKAC	1 cut(s)	796
AccII	CGCG	1 cut(s)	534
AccIII	TCCGGA	1 cut(s)	1754
AciI	CCGC	3 cut(s)	534, 875, 1652
AclWI	GGATC	4 cut(s)	47, 504, 607, 1741
AcsI	RAATTY	1 cut(s)	810
AcuI	CTGAAG	2 cut(s)	471, 1781
AcyI	GRCGYC	1 cut(s)	748
AfaI	GTAC	3 cut(s)	376, 616, 1044
AfiI	CCNNNNNNNGG	4 cut(s)	377, 1052, 1483, 1484
AflII	CTTAAG	1 cut(s)	1466
AflIII	ACRYGT	1 cut(s)	1695
AjnI	CCWGG	2 cut(s)	370, 742
Alw21I	GWGCWC	2 cut(s)	839, 1316
Alw26I	GTCTC	3 cut(s)	678, 1283, 1478
AlwI	GGATC	4 cut(s)	47, 504, 607, 1741
Ama87I	CYCGRG	1 cut(s)	606
Aor13HI	TCCGGA	1 cut(s)	1754
AoxI	GGCC	5 cut(s)	365, 445, 740, 1204, 1662
ApeKI	GCWGC	5 cut(s)	68, 191, 1235, 1655, 1712
ApoI	RAATTY	1 cut(s)	810
AspLEI	GCGC	1 cut(s)	1325
AspS9I	GGNCC	6 cut(s)	365, 446, 1205, 1421, 1459, 1663
AsuHPI	GGTGA	3 cut(s)	485, 1121, 1353
AsuII	TTCGAA	1 cut(s)	280
AvaI	CYCGRG	1 cut(s)	606
AvaII	GGWCC	2 cut(s)	1421, 1459
BaeI	ACNNNNGTAYC	2 cut(s)	606, 639
BanII	GRGCYC	2 cut(s)	532, 839
BbsI	GAAGAC	2 cut(s)	1295, 1647
Bbv12I	GWGCWC	2 cut(s)	839, 1316
BbvI	GCAGC	5 cut(s)	55, 178, 1247, 1642, 1724
BccI	CCATC	4 cut(s)	605, 656, 1688, 1702
BceAI	ACGGC	1 cut(s)	21
BcgI	CGANNNNNNTGC	2 cut(s)	1637, 1671
BciT130I	CCWGG	2 cut(s)	372, 744
BclI	TGATCA	1 cut(s)	520
BcoDI	GTCTC	3 cut(s)	678, 1283, 1478
BfaI	CTAG	2 cut(s)	1209, 1232
BfmI	CTRYAG	4 cut(s)	69, 797, 892, 1656
BfrI	CTTAAG	1 cut(s)	1466
BfuAI	ACCTGC	1 cut(s)	1054
BglI	GCCNNNNNGGC	1 cut(s)	1212
BisI	GCNGC	7 cut(s)	69, 192, 876, 1236, 1653, 1656, 1713
BlsI	GCNGC	7 cut(s)	70, 193, 877, 1237, 1654, 1657, 1714
Bme1390I	CCNGG	2 cut(s)	372, 744
Bme18I	GGWCC	2 cut(s)	1421, 1459
BmeT110I	CYCGRG	1 cut(s)	606
BmgT120I	GGNCC	6 cut(s)	365, 446, 1205, 1421, 1459, 1663
BmiI	GGNNCC	1 cut(s)	1057
BmrFI	CCNGG	2 cut(s)	372, 744
BmrI	ACTGGG	1 cut(s)	627
BmsI	GCATC	8 cut(s)	302, 499, 568, 1070, 1339, 1380, 1588, 1639
BmuI	ACTGGG	1 cut(s)	627
BpiI	GAAGAC	2 cut(s)	1295, 1647
BpmI	CTGGAG	2 cut(s)	834, 1424
Bpu10I	CCTNAGC	2 cut(s)	253, 1716
Bpu14I	TTCGAA	1 cut(s)	280
Bsa29I	ATCGAT	1 cut(s)	1647
BsaBI	GATNNNNATC	1 cut(s)	1772
BsaHI	GRCGYC	1 cut(s)	748
BsaJI	CCNNGG	3 cut(s)	1424, 1726, 1740
BsaWI	WCCGGW	1 cut(s)	1754
BsaXI	ACNNNNNCTCC	2 cut(s)	1014, 1044
Bsc4I	CCNNNNNNNGG	4 cut(s)	377, 1052, 1483, 1484
Bse1I	ACTGG	5 cut(s)	382, 622, 643, 980, 1407
Bse8I	GATNNNNATC	1 cut(s)	1772
BseAI	TCCGGA	1 cut(s)	1754
BseBI	CCWGG	2 cut(s)	372, 744
BseCI	ATCGAT	1 cut(s)	1647
BseDI	CCNNGG	3 cut(s)	1424, 1726, 1740
BseGI	GGATG	7 cut(s)	120, 293, 705, 970, 991, 1354, 1603
BseJI	GATNNNNATC	1 cut(s)	1772
BseLI	CCNNNNNNNGG	4 cut(s)	377, 1052, 1483, 1484
BseMII	CTCAG	5 cut(s)	252, 471, 504, 1278, 1730
BseNI	ACTGG	5 cut(s)	382, 622, 643, 980, 1407
BseRI	GAGGAG	1 cut(s)	300
BseXI	GCAGC	5 cut(s)	55, 178, 1247, 1642, 1724
BseYI	CCCAGC	1 cut(s)	8
BsgI	GTGCAG	3 cut(s)	921, 948, 1212
Bsh1236I	CGCG	1 cut(s)	534
BshFI	GGCC	5 cut(s)	367, 447, 742, 1206, 1664
BshVI	ATCGAT	1 cut(s)	1647
BsiHKAI	GWGCWC	2 cut(s)	839, 1316
BsiHKCI	CYCGRG	1 cut(s)	606
BsiSI	CCGG	1 cut(s)	1755
BslFI	GGGAC	1 cut(s)	93
BslI	CCNNNNNNNGG	4 cut(s)	377, 1052, 1483, 1484
BsmAI	GTCTC	3 cut(s)	678, 1283, 1478
BsmBI	CGTCTC	1 cut(s)	1478
BsmFI	GGGAC	1 cut(s)	93
BsnI	GGCC	5 cut(s)	367, 447, 742, 1206, 1664
BsoBI	CYCGRG	1 cut(s)	606
Bsp119I	TTCGAA	1 cut(s)	280
Bsp1286I	GDGCHC	3 cut(s)	532, 839, 1316
Bsp13I	TCCGGA	1 cut(s)	1754
Bsp143I	GATC	8 cut(s)	39, 496, 520, 538, 599, 967, 1111, 1733
BspACI	CCGC	3 cut(s)	534, 875, 1652
BspANI	GGCC	5 cut(s)	367, 447, 742, 1206, 1664
BspCNI	CTCAG	5 cut(s)	253, 472, 505, 1279, 1729
BspDI	ATCGAT	1 cut(s)	1647
BspEI	TCCGGA	1 cut(s)	1754
BspFNI	CGCG	1 cut(s)	534
BspHI	TCATGA	2 cut(s)	625, 1306
BspLI	GGNNCC	1 cut(s)	1057
BspMAI	CTGCAG	2 cut(s)	73, 1660
BspMI	ACCTGC	1 cut(s)	1054
BspPI	GGATC	4 cut(s)	47, 504, 607, 1741
BspQI	GCTCTTC	1 cut(s)	277
BspT104I	TTCGAA	1 cut(s)	280
BspTI	CTTAAG	1 cut(s)	1466
BsrI	ACTGG	5 cut(s)	382, 622, 643, 980, 1407
BssECI	CCNNGG	3 cut(s)	1424, 1726, 1740
BssMI	GATC	8 cut(s)	39, 496, 520, 538, 599, 967, 1111, 1733
BssNI	GRCGYC	1 cut(s)	748
BssT1I	CCWWGG	2 cut(s)	1424, 1726
Bst2UI	CCWGG	2 cut(s)	372, 744
Bst4CI	ACNGT	7 cut(s)	58, 632, 896, 1243, 1256, 1365, 1455
Bst6I	CTCTTC	3 cut(s)	277, 984, 1755
BstACI	GRCGYC	1 cut(s)	748
BstAFI	CTTAAG	1 cut(s)	1466
BstAPI	GCANNNNNTGC	1 cut(s)	1320
BstBI	TTCGAA	1 cut(s)	280
BstC8I	GCNNGC	3 cut(s)	532, 873, 880
BstDEI	CTNAG	8 cut(s)	253, 261, 480, 513, 956, 1287, 1531, 1716
BstF5I	GGATG	7 cut(s)	120, 293, 705, 970, 991, 1354, 1603
BstFNI	CGCG	1 cut(s)	534
BstHHI	GCGC	1 cut(s)	1325
BstKTI	GATC	8 cut(s)	42, 499, 523, 541, 602, 970, 1114, 1736
BstMAI	GTCTC	3 cut(s)	678, 1283, 1478
BstMBI	GATC	8 cut(s)	39, 496, 520, 538, 599, 967, 1111, 1733
BstMWI	GCNNNNNNNGC	7 cut(s)	266, 1055, 1176, 1212, 1235, 1320, 1661
BstNI	CCWGG	2 cut(s)	372, 744
BstNSI	RCATGY	5 cut(s)	344, 557, 1028, 1194, 1699
BstSCI	CCNGG	2 cut(s)	370, 742
BstSFI	CTRYAG	4 cut(s)	69, 797, 892, 1656
BstUI	CGCG	1 cut(s)	534
BstV1I	GCAGC	5 cut(s)	55, 178, 1247, 1642, 1724
BstV2I	GAAGAC	2 cut(s)	1295, 1647
BstX2I	RGATCY	2 cut(s)	496, 1733
BstXI	CCANNNNNNTGG	1 cut(s)	982
BstYI	RGATCY	2 cut(s)	496, 1733
Bsu15I	ATCGAT	1 cut(s)	1647
BsuRI	GGCC	5 cut(s)	367, 447, 742, 1206, 1664
BsuTUI	ATCGAT	1 cut(s)	1647
BtsCI	GGATG	7 cut(s)	120, 293, 705, 970, 991, 1354, 1603
BtsI	GCAGTG	2 cut(s)	936, 1222
BtsIMutI	CAGTG	7 cut(s)	901, 936, 987, 1062, 1222, 1261, 1451
BveI	ACCTGC	1 cut(s)	1054
Cac8I	GCNNGC	3 cut(s)	532, 873, 880
CciI	TCATGA	2 cut(s)	625, 1306
CfoI	GCGC	1 cut(s)	1325
Cfr13I	GGNCC	6 cut(s)	365, 446, 1205, 1421, 1459, 1663
ClaI	ATCGAT	1 cut(s)	1647
CseI	GACGC	1 cut(s)	1460
CsiI	ACCWGGT	1 cut(s)	370
Csp6I	GTAC	3 cut(s)	375, 615, 1043
CviAII	CATG	9 cut(s)	314, 341, 391, 554, 626, 1025, 1191, 1307, 1696
CviQI	GTAC	3 cut(s)	375, 615, 1043
DdeI	CTNAG	8 cut(s)	253, 261, 480, 513, 956, 1287, 1531, 1716
DpnI	GATC	8 cut(s)	41, 498, 522, 540, 601, 969, 1113, 1735
DpnII	GATC	8 cut(s)	39, 496, 520, 538, 599, 967, 1111, 1733
Eam1104I	CTCTTC	3 cut(s)	277, 984, 1755
EarI	CTCTTC	3 cut(s)	277, 984, 1755
Ecl136II	GAGCTC	1 cut(s)	837
Eco130I	CCWWGG	2 cut(s)	1424, 1726
Eco24I	GRGCYC	2 cut(s)	532, 839
Eco32I	GATATC	1 cut(s)	1443
Eco47I	GGWCC	2 cut(s)	1421, 1459
Eco53kI	GAGCTC	1 cut(s)	837
Eco57I	CTGAAG	2 cut(s)	471, 1781
Eco88I	CYCGRG	1 cut(s)	606
EcoICRI	GAGCTC	1 cut(s)	837
EcoO109I	RGGNCCY	2 cut(s)	1205, 1663
EcoRII	CCWGG	2 cut(s)	370, 742
EcoRV	GATATC	1 cut(s)	1443
EcoT14I	CCWWGG	2 cut(s)	1424, 1726
EcoT22I	ATGCAT	1 cut(s)	1354
EcoT38I	GRGCYC	2 cut(s)	532, 839
ErhI	CCWWGG	2 cut(s)	1424, 1726
Esp3I	CGTCTC	1 cut(s)	1478
FaeI	CATG	9 cut(s)	317, 344, 394, 557, 629, 1028, 1194, 1310, 1699
FaqI	GGGAC	1 cut(s)	93
FatI	CATG	9 cut(s)	313, 340, 390, 553, 625, 1024, 1190, 1306, 1695
FbaI	TGATCA	1 cut(s)	520
FblI	GTMKAC	1 cut(s)	796
Fnu4HI	GCNGC	7 cut(s)	69, 192, 876, 1236, 1653, 1656, 1713
FokI	GGATG	7 cut(s)	127, 280, 712, 977, 998, 1361, 1610
FriOI	GRGCYC	2 cut(s)	532, 839
Fsp4HI	GCNGC	7 cut(s)	69, 192, 876, 1236, 1653, 1656, 1713
FspAI	RTGCGCAY	1 cut(s)	1324
FspBI	CTAG	2 cut(s)	1209, 1232
FspI	TGCGCA	1 cut(s)	1324
GlaI	GCGC	1 cut(s)	1324
GluI	GCNGC	7 cut(s)	69, 192, 876, 1236, 1653, 1656, 1713
GsaI	CCCAGC	1 cut(s)	12
GsuI	CTGGAG	2 cut(s)	834, 1424
HaeIII	GGCC	5 cut(s)	367, 447, 742, 1206, 1664
HapII	CCGG	1 cut(s)	1755
HgaI	GACGC	1 cut(s)	1460
HhaI	GCGC	1 cut(s)	1325
Hin1I	GRCGYC	1 cut(s)	748
Hin1II	CATG	9 cut(s)	317, 344, 394, 557, 629, 1028, 1194, 1310, 1699
Hin6I	GCGC	1 cut(s)	1323
HinP1I	GCGC	1 cut(s)	1323
HinfI	GANTC	3 cut(s)	671, 802, 1535
HpaII	CCGG	1 cut(s)	1755
HphI	GGTGA	3 cut(s)	485, 1121, 1353
Hpy166II	GTNNAC	3 cut(s)	640, 797, 859
Hpy188I	TCNGA	2 cut(s)	543, 945
Hpy8I	GTNNAC	3 cut(s)	640, 797, 859
HpyCH4III	ACNGT	7 cut(s)	58, 632, 896, 1243, 1256, 1365, 1455
HpyCH4IV	ACGT	2 cut(s)	748, 1592
HpyF10VI	GCNNNNNNNGC	7 cut(s)	266, 1055, 1176, 1212, 1235, 1320, 1661
HpyF3I	CTNAG	8 cut(s)	253, 261, 480, 513, 956, 1287, 1531, 1716
HpySE526I	ACGT	2 cut(s)	748, 1592
Hsp92I	GRCGYC	1 cut(s)	748
Hsp92II	CATG	9 cut(s)	317, 344, 394, 557, 629, 1028, 1194, 1310, 1699
HspAI	GCGC	1 cut(s)	1323
Kpn2I	TCCGGA	1 cut(s)	1754
Ksp22I	TGATCA	1 cut(s)	520
Kzo9I	GATC	8 cut(s)	39, 496, 520, 538, 599, 967, 1111, 1733
LguI	GCTCTTC	1 cut(s)	277
LmnI	GCTCC	3 cut(s)	853, 1055, 1153
Lsp1109I	GCAGC	5 cut(s)	55, 178, 1247, 1642, 1724
LweI	GCATC	8 cut(s)	302, 499, 568, 1070, 1339, 1380, 1588, 1639
MabI	ACCWGGT	1 cut(s)	370
MaeI	CTAG	2 cut(s)	1209, 1232
MaeII	ACGT	2 cut(s)	748, 1592
MaeIII	GTNAC	8 cut(s)	316, 545, 645, 1003, 1186, 1398, 1564, 1614
MalI	GATC	8 cut(s)	41, 498, 522, 540, 601, 969, 1113, 1735
MboI	GATC	8 cut(s)	39, 496, 520, 538, 599, 967, 1111, 1733
MfeI	CAATTG	1 cut(s)	1619
MflI	RGATCY	2 cut(s)	496, 1733
MhlI	GDGCHC	3 cut(s)	532, 839, 1316
MlyI	GAGTC	1 cut(s)	680
MmeI	TCCRAC	1 cut(s)	1575
Mph1103I	ATGCAT	1 cut(s)	1354
MroI	TCCGGA	1 cut(s)	1754
MseI	TTAA	9 cut(s)	225, 336, 441, 678, 921, 999, 1089, 1467, 1700
MspCI	CTTAAG	1 cut(s)	1466
MspI	CCGG	1 cut(s)	1755
MspR9I	CCNGG	2 cut(s)	372, 744
MunI	CAATTG	1 cut(s)	1619
MvaI	CCWGG	2 cut(s)	372, 744
MvnI	CGCG	1 cut(s)	534
MwoI	GCNNNNNNNGC	7 cut(s)	266, 1055, 1176, 1212, 1235, 1320, 1661
NdeII	GATC	8 cut(s)	39, 496, 520, 538, 599, 967, 1111, 1733
NlaIII	CATG	9 cut(s)	317, 344, 394, 557, 629, 1028, 1194, 1310, 1699
NlaIV	GGNNCC	1 cut(s)	1057
NmeAIII	GCCGAG	1 cut(s)	1765
NmuCI	GTSAC	4 cut(s)	316, 545, 1564, 1614
NsbI	TGCGCA	1 cut(s)	1324
NsiI	ATGCAT	1 cut(s)	1354
NspI	RCATGY	5 cut(s)	344, 557, 1028, 1194, 1699
NspV	TTCGAA	1 cut(s)	280
PaeR7I	CTCGAG	1 cut(s)	606
PagI	TCATGA	2 cut(s)	625, 1306
PciI	ACATGT	1 cut(s)	1695
PciSI	GCTCTTC	1 cut(s)	277
PfeI	GAWTC	2 cut(s)	802, 1535
PkrI	GCNGC	7 cut(s)	70, 193, 877, 1237, 1654, 1657, 1714
PleI	GAGTC	1 cut(s)	679
PpsI	GAGTC	1 cut(s)	679
PscI	ACATGT	1 cut(s)	1695
Psp124BI	GAGCTC	1 cut(s)	839
Psp6I	CCWGG	2 cut(s)	370, 742
PspFI	CCCAGC	1 cut(s)	8
PspGI	CCWGG	2 cut(s)	370, 742
PspN4I	GGNNCC	1 cut(s)	1057
PspPI	GGNCC	6 cut(s)	365, 446, 1205, 1421, 1459, 1663
PstI	CTGCAG	2 cut(s)	73, 1660
PsuI	RGATCY	2 cut(s)	496, 1733
RsaI	GTAC	3 cut(s)	376, 616, 1044
RsaNI	GTAC	3 cut(s)	375, 615, 1043
SacI	GAGCTC	1 cut(s)	839
SapI	GCTCTTC	1 cut(s)	277
SaqAI	TTAA	9 cut(s)	225, 336, 441, 678, 921, 999, 1089, 1467, 1700
SatI	GCNGC	7 cut(s)	69, 192, 876, 1236, 1653, 1656, 1713
Sau3AI	GATC	8 cut(s)	39, 496, 520, 538, 599, 967, 1111, 1733
Sau96I	GGNCC	6 cut(s)	365, 446, 1205, 1421, 1459, 1663
SchI	GAGTC	1 cut(s)	680
ScrFI	CCNGG	2 cut(s)	372, 744
SduI	GDGCHC	3 cut(s)	532, 839, 1316
SexAI	ACCWGGT	1 cut(s)	370
SfaNI	GCATC	8 cut(s)	302, 499, 568, 1070, 1339, 1380, 1588, 1639
SfcI	CTRYAG	4 cut(s)	69, 797, 892, 1656
Sfr274I	CTCGAG	1 cut(s)	606
SfuI	TTCGAA	1 cut(s)	280
SinI	GGWCC	2 cut(s)	1421, 1459
SlaI	CTCGAG	1 cut(s)	606
SmlI	CTYRAG	2 cut(s)	606, 1466
SmoI	CTYRAG	2 cut(s)	606, 1466
SsiI	CCGC	3 cut(s)	534, 875, 1652
SspMI	CTAG	2 cut(s)	1209, 1232
SstI	GAGCTC	1 cut(s)	839
StyD4I	CCNGG	2 cut(s)	370, 742
StyI	CCWWGG	2 cut(s)	1424, 1726
TaaI	ACNGT	7 cut(s)	58, 632, 896, 1243, 1256, 1365, 1455
TaiI	ACGT	2 cut(s)	751, 1595
TaqI	TCGA	4 cut(s)	280, 607, 1647, 1810
TauI	GCSGC	2 cut(s)	878, 1655
TfiI	GAWTC	2 cut(s)	802, 1535
Tru1I	TTAA	9 cut(s)	225, 336, 441, 678, 921, 999, 1089, 1467, 1700
Tru9I	TTAA	9 cut(s)	225, 336, 441, 678, 921, 999, 1089, 1467, 1700
TscAI	CASTG	7 cut(s)	901, 936, 987, 1069, 1222, 1261, 1458
TseFI	GTSAC	4 cut(s)	316, 545, 1564, 1614
TseI	GCWGC	5 cut(s)	68, 191, 1235, 1655, 1712
Tsp45I	GTSAC	4 cut(s)	316, 545, 1564, 1614
TspRI	CASTG	7 cut(s)	901, 936, 987, 1069, 1222, 1261, 1458
Vha464I	CTTAAG	1 cut(s)	1466
VpaK11BI	GGWCC	2 cut(s)	1421, 1459
XapI	RAATTY	1 cut(s)	810
XceI	RCATGY	5 cut(s)	344, 557, 1028, 1194, 1699
XhoI	CTCGAG	1 cut(s)	606
XmiI	GTMKAC	1 cut(s)	796
XspI	CTAG	2 cut(s)	1209, 1232
ZraI	GACGTC	1 cut(s)	749
Zsp2I	ATGCAT	1 cut(s)	1354

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.