Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003337

Physical Location & Seq

Genomic Coordinates Reverse (-)

44316212 .. 44321542

Gene Structure 5331 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1312500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1725 bp

ATGGCCGGCCGGTTCGGCTTATCCTCCTCTTCGCCAGCGGTGATGATGACCGTCACCAACACCCCCGCCCCAGATCTCGCTCTAACCAATCTCGCCTACTGCTCCCCCACCGATCTCCAGAACTTCGCCATCCCCGGCACCAAGCTCAATCTCGCTTCCGTCGGCGACTCCTTCGTCCTCACCGTCTCTGGTCATCCAAGCATACAGAGCGGCCACATTGCGCTGAACGCAATCCAGCGGCGGCATGCTAGGGTTTCTAGTGGCGATTCTATCTCTGTGAGCAGATTTGTTCCACCGGAGGATTTTAATCTGGCCCTGCTTACGCTGGATTTGGAGTTTGTGAAGAAAGGCACTAAGAGTGAACAGGTTGATGCTGTTGCCCTGTCGAAGCAGCTTAAGAAAAGGTTTATCAACCAGGTCATGACGGCTGGGCAAAGAGTATCATTTGAGTTTCAGGGGATCAACTACATTTTTACTGTCAATCAAGCTGCAGTAGAGGGACAAGAAAAGTCTAATAATGGTATTGAAAGAGGGATGATTGTGAATGATACATATTTCATCTTTGAAACATCAAATGCAAGTGGAATAAAGATTGTAAATCAGCGTGAAGCTGCAAGTAGCAACATTTTCAGGCACAAGGAGTTTAATCTTCAAACGCTTGGAATTGGTGGCTTAGGTGCTGAGTTTGCAGATATATTTCGAAGAGCTTTTGCATCCCGTGTATTCCCTCCTCATGTGACAAATAAATTAGGGATTAAGCATGTAAAGGGTATGCTGTTATATGGGCCACCTGGTACTGGAAAAACTCTCATGGCTCGTCAAATTGGAAAGATGTTGAATGGGAAAGAACCAAAGATTGTTAATGGCCCTGAAGTTTTGAGCAAGTTCGTTGGTGAAACTGAGAAGAATGTTAGGGATCTATTTGCTGATGCTGAGAATGATCAAAGGGCTCGCGGAGATCAGAGTGACTTGCATGTGATAATCTTTGATGAAATTGATGCTATTTGTAAGTCAAGAGGATCAACTCGAGATGGTACTGGGGTTCATGACAGTATTGTGAACCAGTTACTCACGAAGATTGATGGTGTGGAGTCTCTTAATAATGTTTTGCTTATTGGGATGACAAACAGAAAGGATTTGCTTGATGAAGCTCTTTTGAGGCCAGGACGTCTGGAAGTTCAAATTGAGATAAGCCTTCCTGATGAAAATGGTCGTCTACAGATTCTTCAAATTCATACAAACAAGATGAAGGAGAGCTCTTTTCTTGCTCCAGATGTGAACCTTCAAGAGCTTGCGGCTCGCACGAAAAACTACAGTGGTGCAGAACTTGAAGGTGTTGTTAAAAGTGCAGTGTCATTTGCTCTGAATAGACAACTAAGTTTGGATGATCTTACCAAGCCAGTGGATGAAGAGAGCATTAAGGTTACTATGGACGATTTTCTACATGCACTCCAAGAAATTGTACCTGCATTTGGAGCCTCCACTGATGACCTTCAAAGATGCAGACTTAATGGAATGGTGGATTGTGGTGATCGTCATAAGCACATATATCAAAGAGCTATATTATTAGTGGAGCAAGTCAAATTGAGCAAAGGAAGCCCACTTGTTACATGCCTTTTGGAAGGCCCTAGTGGCAGTGGTAAATCTGCACTAGCAGCTACCGTTGGCATTGACAGTGATTTTCCTTATGTCAAGATTGTAAGCTCCCACATTATTTTCTTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005794 GO:0005886 GO:0005911 GO:0009506 GO:0012505 GO:0016020 GO:0030054 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04138 ko04721 ko04727 ko04962 map04138 map04721 map04727 map04962

Pfam Domains

Protein Families

AAA CDC48_2 CDC48_N

Protein Analysis

575

Amino Acids

62.62

Weight (kDa)

6.52

Isoelectric Point (pI)

34.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CDC48_N	PF02359	16 - 95	5e-06	Cell division protein 48 (CDC48), N-terminal domain
CDC48_2	PF02933	123 - 167	4.5e-06	Cell division protein 48 (CDC48), domain 2
AAA	PF00004	258 - 398	1.4e-36	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	425 - 460	2.4e-10	AAA+ lid domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003337)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04910
fragaria_vesca	FvH4_3g09180 FvH4_4g01371 FvH4_4g01380
malus_domestica	MD05G1312500.v1.1 MD05G1339600.v1.1 MD10G1294300.v1.1
prunus_persica	Prupe.4G047600_v2.0.a1
pyrus_communis	pycom05g29040 pycom10g24600
rosa_chinensis	RchiOBHm_Chr4g0419821 RchiOBHm_Chr5g0005931 RchiOBHm_Chr5g0005941 RchiOBHm_Chr5g0008441
rosa_laevigata	RLG00000031340 RLG00000031347 RLG00000031352 RLG00000031389 RLG00000031572
rosa_multiflora	Rmu_sc0000720.1_g000006 Rmu_sc0004326.1_g000001 Rmu_sc0006414.1_g000006
rosa_roxburghii	Rroxscaffold_1G00068050
rosa_rugosa	Rorug04G0435800
rosa_samantha	Rh4AG222000 Rh4BG224200 Rh4CG235100 Rh4DG221000 Rh5AG064700 Rh5BG061700 Rh5CG071500 Rh5CG071600 Rh5CG071800 Rh5CG071900
rosa_wichuraiana	Rw4G019100 Rw5G005630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	173
AatII	GACGTC	1 cut(s)	1171
Acc36I	ACCTGC	1 cut(s)	1474
AccB1I	GGYRCC	1 cut(s)	137
AccBSI	CCGCTC	1 cut(s)	210
AccI	GTMKAC	1 cut(s)	1216
AccII	CGCG	1 cut(s)	954
AciI	CCGC	7 cut(s)	38, 66, 210, 238, 241, 954, 1295
AclWI	GGATC	3 cut(s)	467, 924, 1027
AcoI	YGGCCR	3 cut(s)	3, 7, 211
AcsI	RAATTY	1 cut(s)	1230
AcuI	CTGAAG	1 cut(s)	891
AcyI	GRCGYC	1 cut(s)	1168
AfaI	GTAC	3 cut(s)	796, 1036, 1464
AfiI	CCNNNNNNNGG	2 cut(s)	797, 1472
AflII	CTTAAG	1 cut(s)	395
AgsI	TTSAA	9 cut(s)	527, 566, 653, 838, 1181, 1229, 1286, 1331, 1496
AjnI	CCWGG	3 cut(s)	414, 790, 1162
Alw21I	GWGCWC	1 cut(s)	1259
Alw26I	GTCTC	2 cut(s)	190, 1098
AlwI	GGATC	3 cut(s)	467, 924, 1027
Ama87I	CYCGRG	1 cut(s)	1026
AoxI	GGCC	8 cut(s)	3, 7, 211, 312, 785, 865, 1160, 1624
ApeKI	GCWGC	4 cut(s)	391, 488, 611, 1655
ApoI	RAATTY	1 cut(s)	1230
AspLEI	GCGC	1 cut(s)	223
AspS9I	GGNCC	4 cut(s)	313, 785, 866, 1625
AsuC2I	CCSGG	1 cut(s)	135
AsuHPI	GGTGA	5 cut(s)	46, 52, 172, 905, 1541
AsuII	TTCGAA	1 cut(s)	700
AvaI	CYCGRG	1 cut(s)	1026
BaeI	ACNNNNGTAYC	2 cut(s)	1026, 1059
BanI	GGYRCC	1 cut(s)	137
BanII	GRGCYC	2 cut(s)	952, 1259
Bbv12I	GWGCWC	1 cut(s)	1259
BbvI	GCAGC	4 cut(s)	403, 475, 598, 1667
BccI	CCATC	3 cut(s)	137, 1025, 1076
BceAI	ACGGC	1 cut(s)	441
BciT130I	CCWGG	3 cut(s)	416, 792, 1164
BclI	TGATCA	1 cut(s)	940
BcnI	CCSGG	1 cut(s)	135
BcoDI	GTCTC	2 cut(s)	190, 1098
BfaI	CTAG	4 cut(s)	249, 258, 1629, 1652
BfmI	CTRYAG	3 cut(s)	489, 1217, 1312
BfrI	CTTAAG	1 cut(s)	395
BfuAI	ACCTGC	1 cut(s)	1474
BglI	GCCNNNNNGGC	2 cut(s)	15, 1632
BglII	AGATCT	1 cut(s)	73
BisI	GCNGC	8 cut(s)	211, 239, 242, 392, 489, 612, 1296, 1656
BlsI	GCNGC	8 cut(s)	212, 240, 243, 393, 490, 613, 1297, 1657
Bme1390I	CCNGG	4 cut(s)	135, 416, 792, 1164
BmeT110I	CYCGRG	1 cut(s)	1026
BmgT120I	GGNCC	4 cut(s)	313, 785, 866, 1625
BmiI	GGNNCC	2 cut(s)	139, 1477
BmrFI	CCNGG	4 cut(s)	135, 416, 792, 1164
BmrI	ACTGGG	1 cut(s)	1047
BmsI	GCATC	5 cut(s)	361, 722, 919, 988, 1490
BmuI	ACTGGG	1 cut(s)	1047
BpmI	CTGGAG	2 cut(s)	101, 1254
Bpu10I	CCTNAGC	1 cut(s)	673
Bpu14I	TTCGAA	1 cut(s)	700
BpuMI	CCSGG	1 cut(s)	135
BsaBI	GATNNNNATC	1 cut(s)	306
BsaHI	GRCGYC	1 cut(s)	1168
BsaJI	CCNNGG	1 cut(s)	133
BsaWI	WCCGGW	1 cut(s)	295
BsaXI	ACNNNNNCTCC	2 cut(s)	1434, 1464
Bsc4I	CCNNNNNNNGG	2 cut(s)	797, 1472
Bse118I	RCCGGY	2 cut(s)	5, 9
Bse1I	ACTGG	4 cut(s)	802, 1042, 1063, 1400
Bse3DI	GCAATG	1 cut(s)	216
Bse8I	GATNNNNATC	1 cut(s)	306
BseBI	CCWGG	3 cut(s)	416, 792, 1164
BseDI	CCNNGG	1 cut(s)	133
BseGI	GGATG	7 cut(s)	129, 193, 540, 713, 1125, 1390, 1411
BseJI	GATNNNNATC	1 cut(s)	306
BseLI	CCNNNNNNNGG	2 cut(s)	797, 1472
BseMI	GCAATG	1 cut(s)	216
BseMII	CTCAG	3 cut(s)	672, 891, 924
BseNI	ACTGG	4 cut(s)	802, 1042, 1063, 1400
BseRI	GAGGAG	2 cut(s)	16, 720
BseX3I	CGGCCG	1 cut(s)	7
BseXI	GCAGC	4 cut(s)	403, 475, 598, 1667
BseYI	CCCAGC	1 cut(s)	428
BsgI	GTGCAG	3 cut(s)	1341, 1368, 1632
Bsh1236I	CGCG	1 cut(s)	954
Bsh1285I	CGRYCG	1 cut(s)	10
BshFI	GGCC	8 cut(s)	5, 9, 213, 314, 787, 867, 1162, 1626
BshNI	GGYRCC	1 cut(s)	137
BsiEI	CGRYCG	1 cut(s)	10
BsiHKAI	GWGCWC	1 cut(s)	1259
BsiHKCI	CYCGRG	1 cut(s)	1026
BsiSI	CCGG	4 cut(s)	6, 10, 135, 296
BslFI	GGGAC	1 cut(s)	513
BslI	CCNNNNNNNGG	2 cut(s)	797, 1472
BsmAI	GTCTC	2 cut(s)	190, 1098
BsmBI	CGTCTC	1 cut(s)	190
BsmFI	GGGAC	1 cut(s)	513
BsnI	GGCC	8 cut(s)	5, 9, 213, 314, 787, 867, 1162, 1626
BsoBI	CYCGRG	1 cut(s)	1026
Bsp119I	TTCGAA	1 cut(s)	700
Bsp1286I	GDGCHC	2 cut(s)	952, 1259
Bsp143I	GATC	9 cut(s)	73, 112, 459, 916, 940, 958, 1019, 1387, 1531
BspACI	CCGC	7 cut(s)	38, 66, 210, 238, 241, 954, 1295
BspANI	GGCC	8 cut(s)	5, 9, 213, 314, 787, 867, 1162, 1626
BspCNI	CTCAG	3 cut(s)	673, 892, 925
BspFNI	CGCG	1 cut(s)	954
BspHI	TCATGA	2 cut(s)	420, 1045
BspLI	GGNNCC	2 cut(s)	139, 1477
BspMAI	CTGCAG	1 cut(s)	493
BspMI	ACCTGC	1 cut(s)	1474
BspPI	GGATC	3 cut(s)	467, 924, 1027
BspQI	GCTCTTC	1 cut(s)	697
BspT104I	TTCGAA	1 cut(s)	700
BspT107I	GGYRCC	1 cut(s)	137
BspTI	CTTAAG	1 cut(s)	395
BsrBI	CCGCTC	1 cut(s)	210
BsrDI	GCAATG	1 cut(s)	216
BsrFI	RCCGGY	2 cut(s)	5, 9
BsrI	ACTGG	4 cut(s)	802, 1042, 1063, 1400
BssAI	RCCGGY	2 cut(s)	5, 9
BssECI	CCNNGG	1 cut(s)	133
BssMI	GATC	9 cut(s)	73, 112, 459, 916, 940, 958, 1019, 1387, 1531
BssNI	GRCGYC	1 cut(s)	1168
Bst2UI	CCWGG	3 cut(s)	416, 792, 1164
Bst4CI	ACNGT	7 cut(s)	52, 184, 478, 1052, 1316, 1663, 1676
Bst6I	CTCTTC	3 cut(s)	34, 697, 1404
BstACI	GRCGYC	1 cut(s)	1168
BstAFI	CTTAAG	1 cut(s)	395
BstBI	TTCGAA	1 cut(s)	700
BstC8I	GCNNGC	6 cut(s)	7, 36, 246, 952, 1293, 1300
BstDEI	CTNAG	6 cut(s)	354, 673, 681, 900, 933, 1376
BstF5I	GGATG	7 cut(s)	129, 193, 540, 713, 1125, 1390, 1411
BstFNI	CGCG	1 cut(s)	954
BstHHI	GCGC	1 cut(s)	223
BstKTI	GATC	9 cut(s)	76, 115, 462, 919, 943, 961, 1022, 1390, 1534
BstMAI	GTCTC	2 cut(s)	190, 1098
BstMBI	GATC	9 cut(s)	73, 112, 459, 916, 940, 958, 1019, 1387, 1531
BstMCI	CGRYCG	1 cut(s)	10
BstMWI	GCNNNNNNNGC	8 cut(s)	15, 207, 227, 686, 1475, 1596, 1632, 1655
BstNI	CCWGG	3 cut(s)	416, 792, 1164
BstNSI	RCATGY	5 cut(s)	248, 764, 977, 1448, 1614
BstSCI	CCNGG	4 cut(s)	133, 414, 790, 1162
BstSFI	CTRYAG	3 cut(s)	489, 1217, 1312
BstUI	CGCG	1 cut(s)	954
BstV1I	GCAGC	4 cut(s)	403, 475, 598, 1667
BstX2I	RGATCY	2 cut(s)	73, 916
BstXI	CCANNNNNNTGG	1 cut(s)	1402
BstYI	RGATCY	2 cut(s)	73, 916
BstZI	CGGCCG	1 cut(s)	7
BsuRI	GGCC	8 cut(s)	5, 9, 213, 314, 787, 867, 1162, 1626
BtsCI	GGATG	7 cut(s)	129, 193, 540, 713, 1125, 1390, 1411
BtsI	GCAGTG	2 cut(s)	1356, 1642
BtsIMutI	CAGTG	6 cut(s)	1321, 1356, 1407, 1482, 1642, 1681
BveI	ACCTGC	1 cut(s)	1474
Cac8I	GCNNGC	6 cut(s)	7, 36, 246, 952, 1293, 1300
CciI	TCATGA	2 cut(s)	420, 1045
CfoI	GCGC	1 cut(s)	223
Cfr10I	RCCGGY	2 cut(s)	5, 9
Cfr13I	GGNCC	4 cut(s)	313, 785, 866, 1625
CsiI	ACCWGGT	2 cut(s)	414, 790
Csp6I	GTAC	3 cut(s)	795, 1035, 1463
CviAII	CATG	9 cut(s)	245, 421, 734, 761, 811, 974, 1046, 1445, 1611
CviQI	GTAC	3 cut(s)	795, 1035, 1463
DdeI	CTNAG	6 cut(s)	354, 673, 681, 900, 933, 1376
DpnI	GATC	9 cut(s)	75, 114, 461, 918, 942, 960, 1021, 1389, 1533
DpnII	GATC	9 cut(s)	73, 112, 459, 916, 940, 958, 1019, 1387, 1531
DrdI	GACNNNNNNGTC	1 cut(s)	173
DseDI	GACNNNNNNGTC	1 cut(s)	173
EaeI	YGGCCR	3 cut(s)	3, 7, 211
EagI	CGGCCG	1 cut(s)	7
Eam1104I	CTCTTC	3 cut(s)	34, 697, 1404
EarI	CTCTTC	3 cut(s)	34, 697, 1404
Ecl136II	GAGCTC	1 cut(s)	1257
EclXI	CGGCCG	1 cut(s)	7
Eco24I	GRGCYC	2 cut(s)	952, 1259
Eco52I	CGGCCG	1 cut(s)	7
Eco53kI	GAGCTC	1 cut(s)	1257
Eco57I	CTGAAG	1 cut(s)	891
Eco88I	CYCGRG	1 cut(s)	1026
EcoICRI	GAGCTC	1 cut(s)	1257
EcoO109I	RGGNCCY	1 cut(s)	1625
EcoRII	CCWGG	3 cut(s)	414, 790, 1162
EcoT38I	GRGCYC	2 cut(s)	952, 1259
Esp3I	CGTCTC	1 cut(s)	190
FaeI	CATG	9 cut(s)	248, 424, 737, 764, 814, 977, 1049, 1448, 1614
FaqI	GGGAC	1 cut(s)	513
FatI	CATG	9 cut(s)	244, 420, 733, 760, 810, 973, 1045, 1444, 1610
FauI	CCCGC	1 cut(s)	73
FbaI	TGATCA	1 cut(s)	940
FblI	GTMKAC	1 cut(s)	1216
Fnu4HI	GCNGC	8 cut(s)	211, 239, 242, 392, 489, 612, 1296, 1656
FokI	GGATG	7 cut(s)	116, 180, 547, 700, 1132, 1397, 1418
FriOI	GRGCYC	2 cut(s)	952, 1259
FseI	GGCCGGCC	1 cut(s)	9
Fsp4HI	GCNGC	8 cut(s)	211, 239, 242, 392, 489, 612, 1296, 1656
FspBI	CTAG	4 cut(s)	249, 258, 1629, 1652
GlaI	GCGC	1 cut(s)	222
GluI	GCNGC	8 cut(s)	211, 239, 242, 392, 489, 612, 1296, 1656
GsaI	CCCAGC	1 cut(s)	432
GsuI	CTGGAG	2 cut(s)	101, 1254
HaeIII	GGCC	8 cut(s)	5, 9, 213, 314, 787, 867, 1162, 1626
HapII	CCGG	4 cut(s)	6, 10, 135, 296
HhaI	GCGC	1 cut(s)	223
Hin1I	GRCGYC	1 cut(s)	1168
Hin1II	CATG	9 cut(s)	248, 424, 737, 764, 814, 977, 1049, 1448, 1614
Hin6I	GCGC	1 cut(s)	221
HinP1I	GCGC	1 cut(s)	221
HinfI	GANTC	4 cut(s)	167, 266, 1091, 1222
HpaII	CCGG	4 cut(s)	6, 10, 135, 296
HphI	GGTGA	5 cut(s)	46, 52, 172, 905, 1541
Hpy166II	GTNNAC	4 cut(s)	362, 1060, 1217, 1279
Hpy188I	TCNGA	2 cut(s)	963, 1365
Hpy8I	GTNNAC	4 cut(s)	362, 1060, 1217, 1279
Hpy99I	CGWCG	1 cut(s)	164
HpyAV	CCTTC	7 cut(s)	181, 1205, 1243, 1292, 1325, 1502, 1616
HpyCH4III	ACNGT	7 cut(s)	52, 184, 478, 1052, 1316, 1663, 1676
HpyCH4IV	ACGT	1 cut(s)	1168
HpyF10VI	GCNNNNNNNGC	8 cut(s)	15, 207, 227, 686, 1475, 1596, 1632, 1655
HpyF3I	CTNAG	6 cut(s)	354, 673, 681, 900, 933, 1376
HpySE526I	ACGT	1 cut(s)	1168
Hsp92I	GRCGYC	1 cut(s)	1168
Hsp92II	CATG	9 cut(s)	248, 424, 737, 764, 814, 977, 1049, 1448, 1614
HspAI	GCGC	1 cut(s)	221
KroI	GCCGGC	1 cut(s)	5
KroNI	GCCGGC	1 cut(s)	7
Ksp22I	TGATCA	1 cut(s)	940
Kzo9I	GATC	9 cut(s)	73, 112, 459, 916, 940, 958, 1019, 1387, 1531
LguI	GCTCTTC	1 cut(s)	697
LmnI	GCTCC	5 cut(s)	107, 1273, 1475, 1573, 1709
Lsp1109I	GCAGC	4 cut(s)	403, 475, 598, 1667
LweI	GCATC	5 cut(s)	361, 722, 919, 988, 1490
MabI	ACCWGGT	2 cut(s)	414, 790
MaeI	CTAG	4 cut(s)	249, 258, 1629, 1652
MaeII	ACGT	1 cut(s)	1168
MaeIII	GTNAC	6 cut(s)	52, 736, 965, 1065, 1423, 1606
MalI	GATC	9 cut(s)	75, 114, 461, 918, 942, 960, 1021, 1389, 1533
MbiI	CCGCTC	1 cut(s)	210
MboI	GATC	9 cut(s)	73, 112, 459, 916, 940, 958, 1019, 1387, 1531
MboII	GAAGA	8 cut(s)	21, 355, 641, 714, 916, 1087, 1217, 1421
MflI	RGATCY	2 cut(s)	73, 916
MhlI	GDGCHC	2 cut(s)	952, 1259
MluCI	AATT	8 cut(s)	663, 746, 822, 993, 1182, 1230, 1458, 1583
MlyI	GAGTC	2 cut(s)	161, 1100
MroNI	GCCGGC	1 cut(s)	5
MspA1I	CMGCKG	2 cut(s)	38, 238
MspCI	CTTAAG	1 cut(s)	395
MspI	CCGG	4 cut(s)	6, 10, 135, 296
MspR9I	CCNGG	4 cut(s)	135, 416, 792, 1164
MvaI	CCWGG	3 cut(s)	416, 792, 1164
MvnI	CGCG	1 cut(s)	954
MwoI	GCNNNNNNNGC	8 cut(s)	15, 207, 227, 686, 1475, 1596, 1632, 1655
NaeI	GCCGGC	1 cut(s)	7
NciI	CCSGG	1 cut(s)	135
NdeII	GATC	9 cut(s)	73, 112, 459, 916, 940, 958, 1019, 1387, 1531
NgoMIV	GCCGGC	1 cut(s)	5
NlaIII	CATG	9 cut(s)	248, 424, 737, 764, 814, 977, 1049, 1448, 1614
NlaIV	GGNNCC	2 cut(s)	139, 1477
NmuCI	GTSAC	3 cut(s)	52, 736, 965
NspI	RCATGY	5 cut(s)	248, 764, 977, 1448, 1614
NspV	TTCGAA	1 cut(s)	700
PaeI	GCATGC	1 cut(s)	248
PaeR7I	CTCGAG	1 cut(s)	1026
PagI	TCATGA	2 cut(s)	420, 1045
PciSI	GCTCTTC	1 cut(s)	697
PcsI	WCGNNNNNNNCGW	1 cut(s)	180
PdiI	GCCGGC	1 cut(s)	7
PfeI	GAWTC	2 cut(s)	266, 1222
PkrI	GCNGC	8 cut(s)	212, 240, 243, 393, 490, 613, 1297, 1657
PleI	GAGTC	2 cut(s)	161, 1099
PpsI	GAGTC	2 cut(s)	161, 1099
Psp124BI	GAGCTC	1 cut(s)	1259
Psp6I	CCWGG	3 cut(s)	414, 790, 1162
PspFI	CCCAGC	1 cut(s)	428
PspGI	CCWGG	3 cut(s)	414, 790, 1162
PspN4I	GGNNCC	2 cut(s)	139, 1477
PspPI	GGNCC	4 cut(s)	313, 785, 866, 1625
PstI	CTGCAG	1 cut(s)	493
PsuI	RGATCY	2 cut(s)	73, 916
RigI	GGCCGGCC	1 cut(s)	9
RsaI	GTAC	3 cut(s)	796, 1036, 1464
RsaNI	GTAC	3 cut(s)	795, 1035, 1463
SacI	GAGCTC	1 cut(s)	1259
SapI	GCTCTTC	1 cut(s)	697
SatI	GCNGC	8 cut(s)	211, 239, 242, 392, 489, 612, 1296, 1656
Sau3AI	GATC	9 cut(s)	73, 112, 459, 916, 940, 958, 1019, 1387, 1531
Sau96I	GGNCC	4 cut(s)	313, 785, 866, 1625
SchI	GAGTC	2 cut(s)	161, 1100
ScrFI	CCNGG	4 cut(s)	135, 416, 792, 1164
SduI	GDGCHC	2 cut(s)	952, 1259
SexAI	ACCWGGT	2 cut(s)	414, 790
SfaNI	GCATC	5 cut(s)	361, 722, 919, 988, 1490
SfcI	CTRYAG	3 cut(s)	489, 1217, 1312
Sfr274I	CTCGAG	1 cut(s)	1026
SfuI	TTCGAA	1 cut(s)	700
SlaI	CTCGAG	1 cut(s)	1026
SmlI	CTYRAG	2 cut(s)	395, 1026
SmoI	CTYRAG	2 cut(s)	395, 1026
SphI	GCATGC	1 cut(s)	248
Sse9I	AATT	8 cut(s)	663, 746, 822, 993, 1182, 1230, 1458, 1583
SsiI	CCGC	7 cut(s)	38, 66, 210, 238, 241, 954, 1295
SspMI	CTAG	4 cut(s)	249, 258, 1629, 1652
SstI	GAGCTC	1 cut(s)	1259
StyD4I	CCNGG	4 cut(s)	133, 414, 790, 1162
TaaI	ACNGT	7 cut(s)	52, 184, 478, 1052, 1316, 1663, 1676
TaiI	ACGT	1 cut(s)	1171
TaqI	TCGA	3 cut(s)	386, 700, 1027
TasI	AATT	8 cut(s)	663, 746, 822, 993, 1182, 1230, 1458, 1583
TauI	GCSGC	4 cut(s)	213, 241, 244, 1298
TfiI	GAWTC	2 cut(s)	266, 1222
TscAI	CASTG	6 cut(s)	1321, 1356, 1407, 1489, 1642, 1681
TseFI	GTSAC	3 cut(s)	52, 736, 965
TseI	GCWGC	4 cut(s)	391, 488, 611, 1655
Tsp45I	GTSAC	3 cut(s)	52, 736, 965
TspDTI	ATGAA	8 cut(s)	547, 1005, 1034, 1161, 1218, 1223, 1262, 1422
TspGWI	ACGGA	1 cut(s)	148
TspRI	CASTG	6 cut(s)	1321, 1356, 1407, 1489, 1642, 1681
Vha464I	CTTAAG	1 cut(s)	395
XapI	RAATTY	1 cut(s)	1230
XceI	RCATGY	5 cut(s)	248, 764, 977, 1448, 1614
XhoI	CTCGAG	1 cut(s)	1026
XmiI	GTMKAC	1 cut(s)	1216
XspI	CTAG	4 cut(s)	249, 258, 1629, 1652
ZraI	GACGTC	1 cut(s)	1169

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.