Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0003337

Physical Location & Seq

Genomic Coordinates Forward (+)

4686490 .. 4691662

Gene Structure 5173 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000031389

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1854 bp

ATGGCTCCGACGACGATGATCGTCACCAACACTCTGGCCGCCGACCTTGCTCTCACCAACCTCGCCTACCGCTCGCCGCTCGATCTCCAGACCTTCGCCATTCCCGGCACCAAGCTCAACCTAGCTCTAATCGCCGACTCGTTCAGCATACAGAAGGGTTACATTGCGCTGAATGCGATTCAACGACGGCATGCTAGGGTTTCTAGCGGTGACTCATTATCTGTGAGCAGGTTTACTCCGCCTGATGATTTCAACGTGACCGTGCTCACTCTGGAATTGGAGTTTGTGAAAAAAGGGACTAAGAGTGAACAGATTGATGCTGTTGCCTTGTCGAGCCAACTTAAAAAGAGGTTTATCAACCAGGTTATGACAGCCGGACAAAGAGTATCATTTGAGTACCTTGGGACTAATTACATTTTTACAGTCAATCAAGCTGTAAGATCCGAAGAGTCTAGCTCTGTTGAAAGAGGGATGATCTCGAGTGAAACATACTTCATTTTTGAGGCACCGAGTGGAAATGGAATAAAGATTGTGAACCAACGTTCTGCTACGACTAGCAATATCTTTAAGCACAAGGAATTCAACCTTCAATCACTTGGCATTGGTGGCCTAGGTGCTGAGTTTGCGGATATATTTCGAAGAGCTTTTGCCTCCCGTGTCTTCCCTGCACATGTTACAAATAAACTAGGGATTAAGCATGTGAAGGGTATGCTGCTTTATGGACCACCTGGTACTGGAAAAACTCTTATGGCGCGTCAAATTGGAAAAATGTTGAATGGGAAAGATCCAAAGATTGTTAATGGTCCAGAAGTCCTGAGCAAGTTCGTTGGTGAAACTGAAAAAAATGTTAGGGATCTATTTGCTGATGCTGAGAATGATCAAAGGCTTCGTGCAAAAAAATCACATGTATTTATATATGTTGGATTTAATAGTTTTTGGTTAGACTCAAGAGGATCAACTAGAGATGGCACAAGAGTTCATGATAGTATTGTGAACCAGTTACTCACAAAGATTGATGGTGTGGAATCTCTGAATAATGTTTTGCTTATTGGGATGACCAACAGAAAGGATTTGATTGATGAAGCCCTTTTGAGGCCAGGACGTCTGGAAGTTCAAATTGAGATAAGTCTTCCTGATGAAAATGGTCGCATACAGATTCTTCAAATTCATACGAACAAAATGAAATTGGAGACGAGGATTGGAATTAATTGGAAATTATTAATACGCGCTGCTCGAACAAAGAACTATAGTGGTGCAGAACTGGAAGGTGTTGTTAAAAGTGCAGTATCATTTGCTTTGAATAGACAACTAAGTTTGGATGATCTTACCAAGCCAGTGGATGAAGAGAGTATTAAGGTTACCATGGATGATTTTCTACGTGCACTCTGTGAAATTGTACCTGCATTTGGTGCCTCCACTGATGACCTTGAAAGATGCAGACTTCATGGCATGGTGCATTGTGGGGATCGACATAAGCATATCTATCAAAGAGCCATGCTACTTGTGGAGCAAGTTAAATCAAGTGAGAGAAGCCCTCTTGTTACATGCCTTTTGGAAGGTCCTAGTGGCAGTGGTAAATCTTCACTAGCAGCTACTGTTGGCATCGACAGTGATTTCCCCTATGTCAAGATTATCTCGGCTGAGGCCATGATTGGTCTTCAAGAGAGCACTAAATGTGCACAAATCACAAAGGTTTTTGAAGATGCATACAAGTCACCATTAAGCATTATCATCCTGGACAATATTGAAAGATCAGTTGGTGAAAATTCACTGACCTCACACCAATCCAAGCAGTGGATAGCTTCCTGTGGATTTTTGTTGTTTTCATATCTAGCAATGCTCCTCTCAAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005794 GO:0005886 GO:0005911 GO:0009506 GO:0012505 GO:0016020 GO:0030054 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04138 ko04721 ko04727 ko04962 map04138 map04721 map04727 map04962

Pfam Domains

Protein Families

AAA CDC48_2 CDC48_N

Protein Analysis

618

Amino Acids

68.22

Weight (kDa)

8.96

Isoelectric Point (pI)

34.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CDC48_2	PF02933	104 - 148	9.7e-07	Cell division protein 48 (CDC48), domain 2
AAA	PF00004	237 - 376	1.2e-27	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	410 - 438	7.1e-06	AAA+ lid domain
AAA	PF00004	517 - 595	7.1e-07	ATPase family associated with various cellular activities (AAA)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003337)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04910
fragaria_vesca	FvH4_3g09180 FvH4_4g01371 FvH4_4g01380
malus_domestica	MD05G1312500.v1.1 MD05G1339600.v1.1 MD10G1294300.v1.1
prunus_persica	Prupe.4G047600_v2.0.a1
pyrus_communis	pycom05g29040 pycom10g24600
rosa_chinensis	RchiOBHm_Chr4g0419821 RchiOBHm_Chr5g0005931 RchiOBHm_Chr5g0005941 RchiOBHm_Chr5g0008441
rosa_laevigata	RLG00000031340 RLG00000031347 RLG00000031352 RLG00000031389 RLG00000031572
rosa_multiflora	Rmu_sc0000720.1_g000006 Rmu_sc0004326.1_g000001 Rmu_sc0006414.1_g000006
rosa_roxburghii	Rroxscaffold_1G00068050
rosa_rugosa	Rorug04G0435800
rosa_samantha	Rh4AG222000 Rh4BG224200 Rh4CG235100 Rh4DG221000 Rh5AG064700 Rh5BG061700 Rh5CG071500 Rh5CG071600 Rh5CG071800 Rh5CG071900
rosa_wichuraiana	Rw4G019100 Rw5G005630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1105
Acc36I	ACCTGC	2 cut(s)	219, 1408
AccB1I	GGYRCC	3 cut(s)	107, 505, 1409
AccB7I	CCANNNNNTGG	1 cut(s)	1794
AccBSI	CCGCTC	2 cut(s)	72, 79
AccII	CGCG	2 cut(s)	754, 1227
AciI	CCGC	6 cut(s)	39, 70, 77, 207, 239, 626
AclI	AACGTT	1 cut(s)	541
AclWI	GGATC	5 cut(s)	435, 779, 861, 961, 1473
AcoI	YGGCCR	1 cut(s)	36
AcsI	RAATTY	3 cut(s)	578, 1164, 1765
AcyI	GRCGYC	1 cut(s)	1102
AdeI	CACNNNGTG	2 cut(s)	512, 1388
AfaI	GTAC	3 cut(s)	398, 733, 1398
AfiI	CCNNNNNNNGG	4 cut(s)	734, 1092, 1406, 1794
AflIII	ACRYGT	2 cut(s)	670, 904
AjnI	CCWGG	4 cut(s)	360, 727, 1096, 1734
AjuI	GAANNNNNNNTTGG	2 cut(s)	1740, 1772
AluBI	AGCT	7 cut(s)	115, 125, 434, 456, 644, 1592, 1802
AluI	AGCT	7 cut(s)	115, 125, 434, 456, 644, 1592, 1802
Alw21I	GWGCWC	4 cut(s)	267, 1384, 1670, 1681
Alw26I	GTCTC	1 cut(s)	1184
Alw44I	GTGCAC	2 cut(s)	1380, 1677
AlwI	GGATC	5 cut(s)	435, 779, 861, 961, 1473
AlwNI	CAGNNNCTG	1 cut(s)	1595
Ama87I	CYCGRG	1 cut(s)	478
AoxI	GGCC	4 cut(s)	36, 607, 1094, 1644
ApaLI	GTGCAC	2 cut(s)	1380, 1677
ApeKI	GCWGC	3 cut(s)	712, 1229, 1589
ApoI	RAATTY	3 cut(s)	578, 1164, 1765
AseI	ATTAAT	2 cut(s)	1206, 1220
AspA2I	CCTAGG	1 cut(s)	610
AspLEI	GCGC	3 cut(s)	169, 754, 1229
AspS9I	GGNCC	3 cut(s)	722, 803, 1559
AsuC2I	CCSGG	1 cut(s)	105
AsuHPI	GGTGA	6 cut(s)	16, 46, 221, 842, 1707, 1772
AsuII	TTCGAA	1 cut(s)	637
AvaI	CYCGRG	1 cut(s)	478
AvaII	GGWCC	3 cut(s)	722, 803, 1559
AvrII	CCTAGG	1 cut(s)	610
BaeGI	GKGCMC	2 cut(s)	1384, 1681
BanI	GGYRCC	3 cut(s)	107, 505, 1409
BbsI	GAAGAC	3 cut(s)	652, 1121, 1649
Bbv12I	GWGCWC	4 cut(s)	267, 1384, 1670, 1681
BbvCI	CCTCAGC	1 cut(s)	1641
BbvI	GCAGC	3 cut(s)	699, 1216, 1601
BccI	CCATC	2 cut(s)	959, 1010
BceAI	ACGGC	1 cut(s)	203
BciT130I	CCWGG	4 cut(s)	362, 729, 1098, 1736
BclI	TGATCA	1 cut(s)	877
BcnI	CCSGG	1 cut(s)	105
BcoDI	GTCTC	1 cut(s)	1184
BfmI	CTRYAG	1 cut(s)	1246
BfuAI	ACCTGC	2 cut(s)	219, 1408
BisI	GCNGC	5 cut(s)	39, 77, 713, 1230, 1590
BlnI	CCTAGG	1 cut(s)	610
BlsI	GCNGC	5 cut(s)	40, 78, 714, 1231, 1591
Bme1390I	CCNGG	5 cut(s)	105, 362, 729, 1098, 1736
Bme18I	GGWCC	3 cut(s)	722, 803, 1559
BmeT110I	CYCGRG	1 cut(s)	478
BmgT120I	GGNCC	3 cut(s)	722, 803, 1559
BmiI	GGNNCC	4 cut(s)	6, 109, 507, 1411
BmrFI	CCNGG	5 cut(s)	105, 362, 729, 1098, 1736
BmsI	GCATC	5 cut(s)	307, 856, 1424, 1611, 1693
BpiI	GAAGAC	3 cut(s)	652, 1121, 1649
BplI	GAGNNNNNCTC	4 cut(s)	440, 472, 1519, 1551
BpmI	CTGGAG	1 cut(s)	71
Bpu10I	CCTNAGC	2 cut(s)	815, 1641
Bpu14I	TTCGAA	1 cut(s)	637
BpuEI	CTTGAG	2 cut(s)	931, 1831
BpuMI	CCSGG	1 cut(s)	105
BsaAI	YACGTR	1 cut(s)	1379
BsaHI	GRCGYC	1 cut(s)	1102
BsaJI	CCNNGG	3 cut(s)	400, 610, 1362
Bsc4I	CCNNNNNNNGG	4 cut(s)	734, 1092, 1406, 1794
Bse1I	ACTGG	4 cut(s)	739, 997, 1266, 1334
Bse3DI	GCAATG	2 cut(s)	162, 1842
BseBI	CCWGG	4 cut(s)	362, 729, 1098, 1736
BseDI	CCNNGG	3 cut(s)	400, 610, 1362
BseGI	GGATG	6 cut(s)	477, 1059, 1324, 1345, 1372, 1731
BseLI	CCNNNNNNNGG	4 cut(s)	734, 1092, 1406, 1794
BseMI	GCAATG	2 cut(s)	162, 1842
BseMII	CTCAG	4 cut(s)	609, 806, 861, 1632
BseNI	ACTGG	4 cut(s)	739, 997, 1266, 1334
BseRI	GAGGAG	1 cut(s)	1832
BseSI	GKGCMC	2 cut(s)	1384, 1681
BseXI	GCAGC	3 cut(s)	699, 1216, 1601
BsgI	GTGCAG	3 cut(s)	651, 1275, 1302
Bsh1236I	CGCG	2 cut(s)	754, 1227
BshFI	GGCC	4 cut(s)	38, 609, 1096, 1646
BshNI	GGYRCC	3 cut(s)	107, 505, 1409
BsiHKAI	GWGCWC	4 cut(s)	267, 1384, 1670, 1681
BsiHKCI	CYCGRG	1 cut(s)	478
BsiSI	CCGG	2 cut(s)	105, 375
BslFI	GGGAC	2 cut(s)	310, 418
BslI	CCNNNNNNNGG	4 cut(s)	734, 1092, 1406, 1794
BsmAI	GTCTC	1 cut(s)	1184
BsmBI	CGTCTC	1 cut(s)	1184
BsmFI	GGGAC	2 cut(s)	310, 418
BsmI	GAATGC	1 cut(s)	178
BsnI	GGCC	4 cut(s)	38, 609, 1096, 1646
BsoBI	CYCGRG	1 cut(s)	478
Bsp119I	TTCGAA	1 cut(s)	637
Bsp1286I	GDGCHC	4 cut(s)	267, 1384, 1670, 1681
Bsp19I	CCATGG	1 cut(s)	1362
BspACI	CCGC	6 cut(s)	39, 70, 77, 207, 239, 626
BspANI	GGCC	4 cut(s)	38, 609, 1096, 1646
BspCNI	CTCAG	4 cut(s)	610, 807, 862, 1633
BspFNI	CGCG	2 cut(s)	754, 1227
BspHI	TCATGA	1 cut(s)	979
BspLI	GGNNCC	4 cut(s)	6, 109, 507, 1411
BspMI	ACCTGC	2 cut(s)	219, 1408
BspPI	GGATC	5 cut(s)	435, 779, 861, 961, 1473
BspQI	GCTCTTC	1 cut(s)	634
BspT104I	TTCGAA	1 cut(s)	637
BspT107I	GGYRCC	3 cut(s)	107, 505, 1409
BsrBI	CCGCTC	2 cut(s)	72, 79
BsrDI	GCAATG	2 cut(s)	162, 1842
BsrI	ACTGG	4 cut(s)	739, 997, 1266, 1334
BssECI	CCNNGG	3 cut(s)	400, 610, 1362
BssNI	GRCGYC	1 cut(s)	1102
BssT1I	CCWWGG	3 cut(s)	400, 610, 1362
Bst2UI	CCWGG	4 cut(s)	362, 729, 1098, 1736
Bst4CI	ACNGT	4 cut(s)	262, 424, 1597, 1610
Bst6I	CTCTTC	3 cut(s)	441, 634, 1338
BstACI	GRCGYC	1 cut(s)	1102
BstAPI	GCANNNNNTGC	1 cut(s)	1409
BstBAI	YACGTR	1 cut(s)	1379
BstBI	TTCGAA	1 cut(s)	637
BstC8I	GCNNGC	2 cut(s)	74, 192
BstDEI	CTNAG	6 cut(s)	300, 618, 815, 870, 1310, 1641
BstDSI	CCRYGG	1 cut(s)	1362
BstEII	GGTNACC	1 cut(s)	1357
BstF5I	GGATG	6 cut(s)	477, 1059, 1324, 1345, 1372, 1731
BstFNI	CGCG	2 cut(s)	754, 1227
BstHHI	GCGC	3 cut(s)	169, 754, 1229
BstMAI	GTCTC	1 cut(s)	1184
BstMWI	GCNNNNNNNGC	6 cut(s)	47, 131, 173, 606, 623, 1409
BstNI	CCWGG	4 cut(s)	362, 729, 1098, 1736
BstNSI	RCATGY	5 cut(s)	194, 674, 701, 908, 1548
BstPI	GGTNACC	1 cut(s)	1357
BstSCI	CCNGG	5 cut(s)	103, 360, 727, 1096, 1734
BstSFI	CTRYAG	1 cut(s)	1246
BstSLI	GKGCMC	2 cut(s)	1384, 1681
BstUI	CGCG	2 cut(s)	754, 1227
BstV1I	GCAGC	3 cut(s)	699, 1216, 1601
BstV2I	GAAGAC	3 cut(s)	652, 1121, 1649
BstX2I	RGATCY	3 cut(s)	440, 784, 853
BstXI	CCANNNNNNTGG	2 cut(s)	34, 1336
BstYI	RGATCY	3 cut(s)	440, 784, 853
BsuRI	GGCC	4 cut(s)	38, 609, 1096, 1646
BtgI	CCRYGG	1 cut(s)	1362
BtsCI	GGATG	6 cut(s)	477, 1059, 1324, 1345, 1372, 1731
BtsI	GCAGTG	2 cut(s)	1576, 1799
BtsIMutI	CAGTG	6 cut(s)	1341, 1416, 1576, 1615, 1769, 1799
BveI	ACCTGC	2 cut(s)	219, 1408
Cac8I	GCNNGC	2 cut(s)	74, 192
CaiI	CAGNNNCTG	1 cut(s)	1595
CciI	TCATGA	1 cut(s)	979
CfoI	GCGC	3 cut(s)	169, 754, 1229
Cfr13I	GGNCC	3 cut(s)	722, 803, 1559
CseI	GACGC	1 cut(s)	743
CsiI	ACCWGGT	2 cut(s)	360, 727
Csp6I	GTAC	3 cut(s)	397, 732, 1397
CviQI	GTAC	3 cut(s)	397, 732, 1397
DdeI	CTNAG	6 cut(s)	300, 618, 815, 870, 1310, 1641
DraIII	CACNNNGTG	2 cut(s)	512, 1388
EaeI	YGGCCR	1 cut(s)	36
Eam1104I	CTCTTC	3 cut(s)	441, 634, 1338
EarI	CTCTTC	3 cut(s)	441, 634, 1338
EciI	GGCGGA	1 cut(s)	228
Eco130I	CCWWGG	3 cut(s)	400, 610, 1362
Eco47I	GGWCC	3 cut(s)	722, 803, 1559
Eco88I	CYCGRG	1 cut(s)	478
Eco91I	GGTNACC	1 cut(s)	1357
EcoO109I	RGGNCCY	1 cut(s)	1559
EcoO65I	GGTNACC	1 cut(s)	1357
EcoRI	GAATTC	1 cut(s)	578
EcoRII	CCWGG	4 cut(s)	360, 727, 1096, 1734
EcoT14I	CCWWGG	3 cut(s)	400, 610, 1362
EcoT22I	ATGCAT	1 cut(s)	1708
ErhI	CCWWGG	3 cut(s)	400, 610, 1362
Esp3I	CGTCTC	1 cut(s)	1184
FaqI	GGGAC	2 cut(s)	310, 418
FbaI	TGATCA	1 cut(s)	877
Fnu4HI	GCNGC	5 cut(s)	39, 77, 713, 1230, 1590
FokI	GGATG	6 cut(s)	484, 1066, 1331, 1352, 1379, 1718
Fsp4HI	GCNGC	5 cut(s)	39, 77, 713, 1230, 1590
GlaI	GCGC	3 cut(s)	168, 753, 1228
GluI	GCNGC	5 cut(s)	39, 77, 713, 1230, 1590
GsuI	CTGGAG	1 cut(s)	71
HaeIII	GGCC	4 cut(s)	38, 609, 1096, 1646
HapII	CCGG	2 cut(s)	105, 375
HgaI	GACGC	1 cut(s)	743
HhaI	GCGC	3 cut(s)	169, 754, 1229
Hin1I	GRCGYC	1 cut(s)	1102
Hin6I	GCGC	3 cut(s)	167, 752, 1227
HinP1I	GCGC	3 cut(s)	167, 752, 1227
HinfI	GANTC	7 cut(s)	137, 178, 212, 449, 944, 1025, 1156
HpaII	CCGG	2 cut(s)	105, 375
HphI	GGTGA	6 cut(s)	16, 46, 221, 842, 1707, 1772
Hpy166II	GTNNAC	6 cut(s)	234, 308, 535, 994, 1382, 1679
Hpy188I	TCNGA	3 cut(s)	9, 445, 1032
Hpy8I	GTNNAC	6 cut(s)	234, 308, 535, 994, 1382, 1679
Hpy99I	CGWCG	3 cut(s)	13, 16, 189
HpyAV	CCTTC	6 cut(s)	103, 148, 596, 697, 1259, 1550
HpyCH4III	ACNGT	4 cut(s)	262, 424, 1597, 1610
HpyCH4IV	ACGT	4 cut(s)	255, 541, 1102, 1378
HpyF10VI	GCNNNNNNNGC	6 cut(s)	47, 131, 173, 606, 623, 1409
HpyF3I	CTNAG	6 cut(s)	300, 618, 815, 870, 1310, 1641
HpySE526I	ACGT	4 cut(s)	255, 541, 1102, 1378
Hsp92I	GRCGYC	1 cut(s)	1102
HspAI	GCGC	3 cut(s)	167, 752, 1227
Ksp22I	TGATCA	1 cut(s)	877
LguI	GCTCTTC	1 cut(s)	634
LmnI	GCTCC	3 cut(s)	10, 1507, 1845
Lsp1109I	GCAGC	3 cut(s)	699, 1216, 1601
LweI	GCATC	5 cut(s)	307, 856, 1424, 1611, 1693
MabI	ACCWGGT	2 cut(s)	360, 727
MaeII	ACGT	4 cut(s)	255, 541, 1102, 1378
MaeIII	GTNAC	9 cut(s)	22, 158, 209, 256, 673, 999, 1357, 1540, 1713
MbiI	CCGCTC	2 cut(s)	72, 79
MboII	GAAGA	9 cut(s)	458, 651, 652, 1121, 1151, 1355, 1572, 1649, 1712
MflI	RGATCY	3 cut(s)	440, 784, 853
MhlI	GDGCHC	4 cut(s)	267, 1384, 1670, 1681
MlyI	GAGTC	4 cut(s)	131, 206, 458, 938
MmeI	TCCRAC	2 cut(s)	32, 901
Mph1103I	ATGCAT	1 cut(s)	1708
MspI	CCGG	2 cut(s)	105, 375
MspR9I	CCNGG	5 cut(s)	105, 362, 729, 1098, 1736
Mva1269I	GAATGC	1 cut(s)	178
MvaI	CCWGG	4 cut(s)	362, 729, 1098, 1736
MvnI	CGCG	2 cut(s)	754, 1227
MwoI	GCNNNNNNNGC	6 cut(s)	47, 131, 173, 606, 623, 1409
NciI	CCSGG	1 cut(s)	105
NcoI	CCATGG	1 cut(s)	1362
NlaIV	GGNNCC	4 cut(s)	6, 109, 507, 1411
NmeAIII	GCCGAG	1 cut(s)	1616
NmuCI	GTSAC	4 cut(s)	22, 209, 256, 1713
NsiI	ATGCAT	1 cut(s)	1708
NspI	RCATGY	5 cut(s)	194, 674, 701, 908, 1548
NspV	TTCGAA	1 cut(s)	637
PaeI	GCATGC	1 cut(s)	194
PaeR7I	CTCGAG	1 cut(s)	478
PagI	TCATGA	1 cut(s)	979
PciI	ACATGT	2 cut(s)	670, 904
PciSI	GCTCTTC	1 cut(s)	634
PcsI	WCGNNNNNNNCGW	1 cut(s)	1231
PctI	GAATGC	1 cut(s)	178
PfeI	GAWTC	3 cut(s)	178, 1025, 1156
PflMI	CCANNNNNTGG	1 cut(s)	1794
PfoI	TCCNGGA	1 cut(s)	1734
PkrI	GCNGC	5 cut(s)	40, 78, 714, 1231, 1591
PleI	GAGTC	4 cut(s)	131, 206, 457, 938
PpsI	GAGTC	4 cut(s)	131, 206, 457, 938
Ppu21I	YACGTR	1 cut(s)	1379
PpuMI	RGGWCCY	1 cut(s)	1559
PscI	ACATGT	2 cut(s)	670, 904
PshBI	ATTAAT	2 cut(s)	1206, 1220
Psp1406I	AACGTT	1 cut(s)	541
Psp5II	RGGWCCY	1 cut(s)	1559
Psp6I	CCWGG	4 cut(s)	360, 727, 1096, 1734
PspEI	GGTNACC	1 cut(s)	1357
PspGI	CCWGG	4 cut(s)	360, 727, 1096, 1734
PspN4I	GGNNCC	4 cut(s)	6, 109, 507, 1411
PspPI	GGNCC	3 cut(s)	722, 803, 1559
PspPPI	RGGWCCY	1 cut(s)	1559
PstNI	CAGNNNCTG	1 cut(s)	1595
PsuI	RGATCY	3 cut(s)	440, 784, 853
RsaI	GTAC	3 cut(s)	398, 733, 1398
RsaNI	GTAC	3 cut(s)	397, 732, 1397
SapI	GCTCTTC	1 cut(s)	634
SatI	GCNGC	5 cut(s)	39, 77, 713, 1230, 1590
Sau96I	GGNCC	3 cut(s)	722, 803, 1559
SchI	GAGTC	4 cut(s)	131, 206, 458, 938
ScrFI	CCNGG	5 cut(s)	105, 362, 729, 1098, 1736
SduI	GDGCHC	4 cut(s)	267, 1384, 1670, 1681
SexAI	ACCWGGT	2 cut(s)	360, 727
SfaNI	GCATC	5 cut(s)	307, 856, 1424, 1611, 1693
SfcI	CTRYAG	1 cut(s)	1246
Sfr274I	CTCGAG	1 cut(s)	478
SfuI	TTCGAA	1 cut(s)	637
SinI	GGWCC	3 cut(s)	722, 803, 1559
SlaI	CTCGAG	1 cut(s)	478
SmlI	CTYRAG	3 cut(s)	478, 946, 1846
SmoI	CTYRAG	3 cut(s)	478, 946, 1846
SphI	GCATGC	1 cut(s)	194
SsiI	CCGC	6 cut(s)	39, 70, 77, 207, 239, 626
SspI	AATATT	1 cut(s)	1744
StyD4I	CCNGG	5 cut(s)	103, 360, 727, 1096, 1734
StyI	CCWWGG	3 cut(s)	400, 610, 1362
TaaI	ACNGT	4 cut(s)	262, 424, 1597, 1610
TaiI	ACGT	4 cut(s)	258, 544, 1105, 1381
TaqI	TCGA	7 cut(s)	81, 332, 479, 637, 1234, 1468, 1605
TauI	GCSGC	2 cut(s)	41, 79
TfiI	GAWTC	3 cut(s)	178, 1025, 1156
TscAI	CASTG	6 cut(s)	1341, 1423, 1576, 1615, 1776, 1799
TseFI	GTSAC	4 cut(s)	22, 209, 256, 1713
TseI	GCWGC	3 cut(s)	712, 1229, 1589
Tsp45I	GTSAC	4 cut(s)	22, 209, 256, 1713
TspDTI	ATGAA	9 cut(s)	484, 968, 1095, 1152, 1157, 1196, 1356, 1433, 1815
TspRI	CASTG	6 cut(s)	1341, 1423, 1576, 1615, 1776, 1799
Van91I	CCANNNNNTGG	1 cut(s)	1794
VneI	GTGCAC	2 cut(s)	1380, 1677
VpaK11BI	GGWCC	3 cut(s)	722, 803, 1559
VspI	ATTAAT	2 cut(s)	1206, 1220
XapI	RAATTY	3 cut(s)	578, 1164, 1765
XceI	RCATGY	5 cut(s)	194, 674, 701, 908, 1548
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1501
XhoI	CTCGAG	1 cut(s)	478
XmaJI	CCTAGG	1 cut(s)	610
ZraI	GACGTC	1 cut(s)	1103
Zsp2I	ATGCAT	1 cut(s)	1708

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.