Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003337

Physical Location & Seq

Genomic Coordinates Reverse (-)

38220072 .. 38226734

Gene Structure 6663 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1294300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2238 bp

ATGGCCGGCCGGTTCGGCTTCTCCTCCTCTCGTCCAGGGGTGATGATGACCGTCACCAACACCCCTGCTGCAGAACTCGCTCTCACCAATCTCGCCTACTGCTCCCCCGCCGATCTACAGAACTTCGCCATCCCCGGCACTAAGCTCAACCTAGCCTCCGTCGGCGACTCCTTCGTCCTCACCGTCTCCGGTCATCCAAGCATACAGAGCGGCCACATTGCGCTAAACGCAATCCAAAGGCGGCATGCTAGGGTTTCTAGTGGCGATTCAGTCTCCGTTAGCAGGTTTGTTCCGCCGGAGGATTTCAGTCTGGCGCTGCTCACGCTGGATTTGGAGTTTGTGAAAAAGGGCACTAAGAGTGAACAGGTTGATGCTGTTCGTCTGTCGGCCCAACTTAAAAAAAGATTTATCAACCAGGTCATGACGGCTGGACAAAGAGTATCATTTGAGTTTCTCGGGATTAACTACATTTTTACTGTCAATCAAGCAGCAGTAGAGGGACAAGAAAAGTCCAATAACGGTATTGAAAGAGGGATGATTGTGAATGATACATATTTCATCTTCGAGACATCAAATGCAAGTGGAATAAAGATCGTAAATCAGCGTGAAGCTGCAAGTAGCAACATTTTCAGGCACAAGGAGTTTAATCTTGAAACACTTGGAATTGGTGGCTTAAGTGCTGAGTTTGCAGATATATTTCGAAGAGCTTTTGCATCCCGTGTATTCCCTCCTCACGTGACAAATAAATTAGGGATCAAGCATGTAAAGGGTATGCTGCTTTATGGGCCCCCTGGTACTGGAAAAACTCTCATGGCTCGTCAAATTGGAAAGATGTTGAACGGGAAAGAACCAAAGATTGTTAATGGCCCTGAAGTTTTGAGCAAGTTCGTTGGTGAAACGGAGAAAAATGTTAGGGATCTATTTGCTGATGCCGAGAATGATCAAAGGGCTCGCGGAGACCAGAGTGATTTGCATGTGATAATCTTTGATGAAATTGATGCTATTTGTAAGTCAAGAGGATCAACTAGAGATGGTACCGGGGTTCATGACAGTATTGTGAACCAGTTACTCACGAAGATTGATGGTGTGGAGTCTCTCAATAATGTTTTGCTTATTGGGATGACTAACAGAAAAGATTTGCTTGATGAAGCCCTTTTGAGGCCAGGACGTTTGGAAGTTCAAATTGAGATAAGCCTTCCTGATGAAAATGGTCGTGTGCAGATTCTTCAGATTCATACAAACAAGATGAAGGAGAGCTCTTTTCTTTCCCCGGATGTAAACCTCCAAGAGCTTGCTGCTCGTACGAAAAACTACAGTGGTGCAGAACTTGAAGGTGTTGTTAAAAGTGCAGTATCATTTGCTCTGAATAGACAACTAAGTTTGGATGATCTTACCAAACCAGTGGATGAAGAGAGTATTAAGGTTACGATGGATGATTTCCTCCATGCACTCCAAGAAATTGTACCTGCATTTGGAGCCTCCACTGATGACCTTCAAAGATGCAGACTTAATGGCATGGTGGATTGTGGTGATCGACATAAGCACATATATCAAAGAGCTATATTACTAGTGGAACAAGTTAAATTGAGCAAAGGAAGCCCACTTGTTACTTGCCTTCTGGAAGGCCCGAGTGGCAGTGGTAAATCTGCATTAGCGGCTACTGTTGGGATCGACAGTGATTTTCCCTACGTCAAGATTGTCTCGGCTGAGACAATGATTGGTCTTCATGAGAGCACCAAATGCGCACAAATTGTAAAGGTTTTCGAGGATGCATACAAGTCACCATTGAGCATCATTATCCTAGATGACATTGAAAGGTTACTGGAGTATGTTGCTATTGGTCCAAGGTTTTCCAATTTAATATCTCAAACATTGTTGGTCCTACTTAAGCGCCTCCCTCCTAAGGGGAAAAAACTTCTGGTATTTGGAACAACAAGTGAATTGGGCTTCTTAGATTCAATCGGCTTTTGTGATACCTTTTCTGTCACTTACAATGTACCCACACTAAAGACGGAGGATGCAAAGAAGGTGCTAGCCCAGTTGAATGTTTTTGCTGACGAAGACATCGATGCGGCTGCAGAGGCACTGAATGATATGCCCATCAAGAAGCTCTACATGTTAATTGAGATGGCAGCCCAGGGTGACCAAGGAGGCTCTGCCGAGGCTATCTACTCCGGAAAAGAGAAGATAAACATCACTCACTTTTTCGATTGCCTTCAGGACATTGTTCGGTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005794 GO:0005886 GO:0005911 GO:0009506 GO:0012505 GO:0016020 GO:0030054 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04138 ko04721 ko04727 ko04962 map04138 map04721 map04727 map04962

Pfam Domains

Protein Families

AAA CDC48_2 CDC48_N

Protein Analysis

746

Amino Acids

81.54

Weight (kDa)

5.88

Isoelectric Point (pI)

32.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CDC48_N	PF02359	15 - 95	1.2e-06	Cell division protein 48 (CDC48), N-terminal domain
CDC48_2	PF02933	123 - 167	3.7e-06	Cell division protein 48 (CDC48), domain 2
AAA	PF00004	258 - 398	2.1e-36	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	425 - 460	3.4e-10	AAA+ lid domain
AAA	PF00004	539 - 652	2.7e-09	ATPase family associated with various cellular activities (AAA)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003337)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04910
fragaria_vesca	FvH4_3g09180 FvH4_4g01371 FvH4_4g01380
malus_domestica	MD05G1312500.v1.1 MD05G1339600.v1.1 MD10G1294300.v1.1
prunus_persica	Prupe.4G047600_v2.0.a1
pyrus_communis	pycom05g29040 pycom10g24600
rosa_chinensis	RchiOBHm_Chr4g0419821 RchiOBHm_Chr5g0005931 RchiOBHm_Chr5g0005941 RchiOBHm_Chr5g0008441
rosa_laevigata	RLG00000031340 RLG00000031347 RLG00000031352 RLG00000031389 RLG00000031572
rosa_multiflora	Rmu_sc0000720.1_g000006 Rmu_sc0004326.1_g000001 Rmu_sc0006414.1_g000006
rosa_roxburghii	Rroxscaffold_1G00068050
rosa_rugosa	Rorug04G0435800
rosa_samantha	Rh4AG222000 Rh4BG224200 Rh4CG235100 Rh4DG221000 Rh5AG064700 Rh5BG061700 Rh5CG071500 Rh5CG071600 Rh5CG071800 Rh5CG071900
rosa_wichuraiana	Rw4G019100 Rw5G005630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	173
Acc16I	TGCGCA	1 cut(s)	1744
Acc36I	ACCTGC	2 cut(s)	273, 1474
Acc65I	GGTACC	1 cut(s)	1034
AccB1I	GGYRCC	1 cut(s)	1034
AccBSI	CCGCTC	1 cut(s)	210
AccII	CGCG	1 cut(s)	954
AccIII	TCCGGA	1 cut(s)	2174
AciI	CCGC	7 cut(s)	108, 210, 241, 293, 954, 1655, 2072
AclWI	GGATC	4 cut(s)	761, 924, 1027, 1676
AcoI	YGGCCR	3 cut(s)	3, 7, 211
AcuI	CTGAAG	3 cut(s)	891, 1211, 2201
AcvI	CACGTG	1 cut(s)	736
AfaI	GTAC	5 cut(s)	796, 1036, 1303, 1464, 1998
AfiI	CCNNNNNNNGG	6 cut(s)	282, 797, 1158, 1472, 1903, 1904
AflII	CTTAAG	2 cut(s)	673, 1886
AflIII	ACRYGT	1 cut(s)	2115
AgsI	TTSAA	9 cut(s)	527, 653, 838, 1181, 1331, 1496, 1814, 1959, 2044
AhlI	ACTAGT	1 cut(s)	1567
AjnI	CCWGG	5 cut(s)	34, 414, 790, 1162, 2136
AluBI	AGCT	7 cut(s)	145, 611, 707, 1257, 1291, 1559, 2110
AluI	AGCT	7 cut(s)	145, 611, 707, 1257, 1291, 1559, 2110
Alw21I	GWGCWC	2 cut(s)	1259, 1736
Alw26I	GTCTC	7 cut(s)	190, 277, 560, 951, 1098, 1703, 1705
AlwI	GGATC	4 cut(s)	761, 924, 1027, 1676
Ama87I	CYCGRG	2 cut(s)	455, 1627
Aor13HI	TCCGGA	1 cut(s)	2174
AoxI	GGCC	8 cut(s)	3, 7, 211, 387, 785, 865, 1160, 1624
ApaI	GGGCCC	1 cut(s)	789
ApeKI	GCWGC	8 cut(s)	68, 316, 488, 611, 775, 1295, 2075, 2132
Asp718I	GGTACC	1 cut(s)	1034
AspLEI	GCGC	4 cut(s)	223, 316, 1745, 1893
AspS9I	GGNCC	7 cut(s)	388, 785, 786, 866, 1625, 1841, 1879
AsuC2I	CCSGG	3 cut(s)	135, 1039, 1271
AsuHPI	GGTGA	8 cut(s)	46, 52, 76, 172, 905, 1541, 1773, 2153
AsuII	TTCGAA	1 cut(s)	700
AsuNHI	GCTAGC	1 cut(s)	2032
AvaI	CYCGRG	2 cut(s)	455, 1627
AvaII	GGWCC	2 cut(s)	1841, 1879
AxyI	CCTNAGG	1 cut(s)	1902
BaeGI	GKGCMC	2 cut(s)	353, 789
BaeI	ACNNNNGTAYC	2 cut(s)	1026, 1059
BanI	GGYRCC	1 cut(s)	1034
BanII	GRGCYC	3 cut(s)	789, 952, 1259
BbrPI	CACGTG	1 cut(s)	736
BbsI	GAAGAC	2 cut(s)	1715, 2067
Bbv12I	GWGCWC	2 cut(s)	1259, 1736
BbvI	GCAGC	8 cut(s)	55, 303, 500, 598, 762, 1282, 2062, 2144
BccI	CCATC	6 cut(s)	137, 1025, 1076, 1423, 2108, 2122
BceAI	ACGGC	1 cut(s)	441
BcgI	CGANNNNNNTGC	2 cut(s)	2057, 2091
BciT130I	CCWGG	5 cut(s)	36, 416, 792, 1164, 2138
BclI	TGATCA	1 cut(s)	940
BcnI	CCSGG	3 cut(s)	135, 1039, 1271
BcoDI	GTCTC	7 cut(s)	190, 277, 560, 951, 1098, 1703, 1705
BcuI	ACTAGT	1 cut(s)	1567
BfaI	CTAG	7 cut(s)	152, 249, 258, 1026, 1568, 1802, 2033
BfmI	CTRYAG	4 cut(s)	69, 116, 1312, 2076
BfoI	RGCGCY	2 cut(s)	317, 1894
BfrI	CTTAAG	2 cut(s)	673, 1886
BfuAI	ACCTGC	2 cut(s)	273, 1474
BglI	GCCNNNNNGGC	2 cut(s)	15, 1632
Bme1390I	CCNGG	8 cut(s)	36, 135, 416, 792, 1039, 1164, 1271, 2138
Bme18I	GGWCC	2 cut(s)	1841, 1879
BmeT110I	CYCGRG	2 cut(s)	455, 1627
BmgT120I	GGNCC	7 cut(s)	388, 785, 786, 866, 1625, 1841, 1879
BmiI	GGNNCC	4 cut(s)	787, 788, 1036, 1477
BmrFI	CCNGG	8 cut(s)	36, 135, 416, 792, 1039, 1164, 1271, 2138
BmrI	ACTGGG	1 cut(s)	2032
BmsI	GCATC	9 cut(s)	361, 722, 919, 988, 1490, 1759, 1800, 2008, 2059
BmtI	GCTAGC	1 cut(s)	2036
BmuI	ACTGGG	1 cut(s)	2032
BpiI	GAAGAC	2 cut(s)	1715, 2067
BpmI	CTGGAG	1 cut(s)	1844
Bpu14I	TTCGAA	1 cut(s)	700
BpuMI	CCSGG	3 cut(s)	135, 1039, 1271
Bsa29I	ATCGAT	1 cut(s)	2067
BsaAI	YACGTR	1 cut(s)	736
BsaBI	GATNNNNATC	1 cut(s)	2192
BsaI	GGTCTC	1 cut(s)	951
BsaWI	WCCGGW	2 cut(s)	188, 2174
BsaXI	ACNNNNNCTCC	2 cut(s)	140, 170
Bsc4I	CCNNNNNNNGG	6 cut(s)	282, 797, 1158, 1472, 1903, 1904
Bse118I	RCCGGY	2 cut(s)	5, 9
Bse1I	ACTGG	5 cut(s)	802, 1063, 1400, 1827, 2038
Bse21I	CCTNAGG	1 cut(s)	1902
Bse3DI	GCAATG	1 cut(s)	216
Bse8I	GATNNNNATC	1 cut(s)	2192
BseAI	TCCGGA	1 cut(s)	2174
BseBI	CCWGG	5 cut(s)	36, 416, 792, 1164, 2138
BseCI	ATCGAT	1 cut(s)	2067
BseJI	GATNNNNATC	1 cut(s)	2192
BseLI	CCNNNNNNNGG	6 cut(s)	282, 797, 1158, 1472, 1903, 1904
BseMI	GCAATG	1 cut(s)	216
BseMII	CTCAG	2 cut(s)	672, 1698
BseNI	ACTGG	5 cut(s)	802, 1063, 1400, 1827, 2038
BseRI	GAGGAG	3 cut(s)	13, 16, 720
BseSI	GKGCMC	2 cut(s)	353, 789
BseX3I	CGGCCG	1 cut(s)	7
BseXI	GCAGC	8 cut(s)	55, 303, 500, 598, 762, 1282, 2062, 2144
BsgI	GTGCAG	3 cut(s)	1238, 1341, 1368
Bsh1236I	CGCG	1 cut(s)	954
Bsh1285I	CGRYCG	1 cut(s)	10
BshFI	GGCC	8 cut(s)	5, 9, 213, 389, 787, 867, 1162, 1626
BshNI	GGYRCC	1 cut(s)	1034
BshVI	ATCGAT	1 cut(s)	2067
BsiEI	CGRYCG	1 cut(s)	10
BsiHKAI	GWGCWC	2 cut(s)	1259, 1736
BsiHKCI	CYCGRG	2 cut(s)	455, 1627
BsiSI	CCGG	8 cut(s)	6, 10, 135, 189, 296, 1038, 1271, 2175
BsiWI	CGTACG	1 cut(s)	1301
BslFI	GGGAC	1 cut(s)	513
BslI	CCNNNNNNNGG	6 cut(s)	282, 797, 1158, 1472, 1903, 1904
BsmAI	GTCTC	7 cut(s)	190, 277, 560, 951, 1098, 1703, 1705
BsmBI	CGTCTC	1 cut(s)	190
BsmFI	GGGAC	1 cut(s)	513
BsnI	GGCC	8 cut(s)	5, 9, 213, 389, 787, 867, 1162, 1626
Bso31I	GGTCTC	1 cut(s)	951
BsoBI	CYCGRG	2 cut(s)	455, 1627
Bsp119I	TTCGAA	1 cut(s)	700
Bsp120I	GGGCCC	1 cut(s)	785
Bsp1286I	GDGCHC	5 cut(s)	353, 789, 952, 1259, 1736
Bsp13I	TCCGGA	1 cut(s)	2174
Bsp143I	GATC	9 cut(s)	112, 591, 753, 916, 940, 1019, 1387, 1531, 1668
BspACI	CCGC	7 cut(s)	108, 210, 241, 293, 954, 1655, 2072
BspANI	GGCC	8 cut(s)	5, 9, 213, 389, 787, 867, 1162, 1626
BspCNI	CTCAG	2 cut(s)	673, 1699
BspDI	ATCGAT	1 cut(s)	2067
BspEI	TCCGGA	1 cut(s)	2174
BspFNI	CGCG	1 cut(s)	954
BspHI	TCATGA	3 cut(s)	420, 1045, 1726
BspLI	GGNNCC	4 cut(s)	787, 788, 1036, 1477
BspMAI	CTGCAG	2 cut(s)	73, 2080
BspMI	ACCTGC	2 cut(s)	273, 1474
BspOI	GCTAGC	1 cut(s)	2036
BspPI	GGATC	4 cut(s)	761, 924, 1027, 1676
BspQI	GCTCTTC	1 cut(s)	697
BspT104I	TTCGAA	1 cut(s)	700
BspT107I	GGYRCC	1 cut(s)	1034
BspTI	CTTAAG	2 cut(s)	673, 1886
BspTNI	GGTCTC	1 cut(s)	951
BsrBI	CCGCTC	1 cut(s)	210
BsrDI	GCAATG	1 cut(s)	216
BsrFI	RCCGGY	2 cut(s)	5, 9
BsrI	ACTGG	5 cut(s)	802, 1063, 1400, 1827, 2038
BssAI	RCCGGY	2 cut(s)	5, 9
BssMI	GATC	9 cut(s)	112, 591, 753, 916, 940, 1019, 1387, 1531, 1668
BssT1I	CCWWGG	2 cut(s)	1844, 2146
Bst2UI	CCWGG	5 cut(s)	36, 416, 792, 1164, 2138
Bst4CI	ACNGT	8 cut(s)	52, 184, 478, 521, 1052, 1316, 1663, 1676
Bst6I	CTCTTC	2 cut(s)	697, 1404
BstAFI	CTTAAG	2 cut(s)	673, 1886
BstAPI	GCANNNNNTGC	1 cut(s)	1740
BstBAI	YACGTR	1 cut(s)	736
BstBI	TTCGAA	1 cut(s)	700
BstC8I	GCNNGC	5 cut(s)	7, 246, 952, 1293, 2034
BstDEI	CTNAG	7 cut(s)	141, 354, 681, 1376, 1707, 1902, 1951
BstEII	GGTNACC	1 cut(s)	2141
BstFNI	CGCG	1 cut(s)	954
BstH2I	RGCGCY	2 cut(s)	317, 1894
BstHHI	GCGC	4 cut(s)	223, 316, 1745, 1893
BstKTI	GATC	9 cut(s)	115, 594, 756, 919, 943, 1022, 1390, 1534, 1671
BstMAI	GTCTC	7 cut(s)	190, 277, 560, 951, 1098, 1703, 1705
BstMBI	GATC	9 cut(s)	112, 591, 753, 916, 940, 1019, 1387, 1531, 1668
BstMCI	CGRYCG	1 cut(s)	10
BstNI	CCWGG	5 cut(s)	36, 416, 792, 1164, 2138
BstNSI	RCATGY	4 cut(s)	248, 764, 977, 2119
BstPI	GGTNACC	1 cut(s)	2141
BstSCI	CCNGG	8 cut(s)	34, 133, 414, 790, 1037, 1162, 1269, 2136
BstSFI	CTRYAG	4 cut(s)	69, 116, 1312, 2076
BstSLI	GKGCMC	2 cut(s)	353, 789
BstUI	CGCG	1 cut(s)	954
BstV1I	GCAGC	8 cut(s)	55, 303, 500, 598, 762, 1282, 2062, 2144
BstV2I	GAAGAC	2 cut(s)	1715, 2067
BstX2I	RGATCY	1 cut(s)	916
BstXI	CCANNNNNNTGG	1 cut(s)	1402
BstYI	RGATCY	1 cut(s)	916
BstZI	CGGCCG	1 cut(s)	7
Bsu15I	ATCGAT	1 cut(s)	2067
Bsu36I	CCTNAGG	1 cut(s)	1902
BsuRI	GGCC	8 cut(s)	5, 9, 213, 389, 787, 867, 1162, 1626
BsuTUI	ATCGAT	1 cut(s)	2067
BtsI	GCAGTG	1 cut(s)	1642
BtsIMutI	CAGTG	6 cut(s)	1321, 1407, 1482, 1642, 1681, 2084
BveI	ACCTGC	2 cut(s)	273, 1474
Cac8I	GCNNGC	5 cut(s)	7, 246, 952, 1293, 2034
CciI	TCATGA	3 cut(s)	420, 1045, 1726
CfoI	GCGC	4 cut(s)	223, 316, 1745, 1893
Cfr10I	RCCGGY	2 cut(s)	5, 9
Cfr13I	GGNCC	7 cut(s)	388, 785, 786, 866, 1625, 1841, 1879
ClaI	ATCGAT	1 cut(s)	2067
CsiI	ACCWGGT	1 cut(s)	414
Csp6I	GTAC	5 cut(s)	795, 1035, 1302, 1463, 1997
CviQI	GTAC	5 cut(s)	795, 1035, 1302, 1463, 1997
DdeI	CTNAG	7 cut(s)	141, 354, 681, 1376, 1707, 1902, 1951
DpnI	GATC	9 cut(s)	114, 593, 755, 918, 942, 1021, 1389, 1533, 1670
DpnII	GATC	9 cut(s)	112, 591, 753, 916, 940, 1019, 1387, 1531, 1668
DrdI	GACNNNNNNGTC	1 cut(s)	173
DseDI	GACNNNNNNGTC	1 cut(s)	173
EaeI	YGGCCR	3 cut(s)	3, 7, 211
EagI	CGGCCG	1 cut(s)	7
Eam1104I	CTCTTC	2 cut(s)	697, 1404
EarI	CTCTTC	2 cut(s)	697, 1404
EciI	GGCGGA	1 cut(s)	282
Ecl136II	GAGCTC	1 cut(s)	1257
EclXI	CGGCCG	1 cut(s)	7
Eco130I	CCWWGG	2 cut(s)	1844, 2146
Eco24I	GRGCYC	3 cut(s)	789, 952, 1259
Eco31I	GGTCTC	1 cut(s)	951
Eco47I	GGWCC	2 cut(s)	1841, 1879
Eco52I	CGGCCG	1 cut(s)	7
Eco53kI	GAGCTC	1 cut(s)	1257
Eco57I	CTGAAG	3 cut(s)	891, 1211, 2201
Eco72I	CACGTG	1 cut(s)	736
Eco81I	CCTNAGG	1 cut(s)	1902
Eco88I	CYCGRG	2 cut(s)	455, 1627
Eco91I	GGTNACC	1 cut(s)	2141
EcoICRI	GAGCTC	1 cut(s)	1257
EcoO109I	RGGNCCY	1 cut(s)	786
EcoO65I	GGTNACC	1 cut(s)	2141
EcoRII	CCWGG	5 cut(s)	34, 414, 790, 1162, 2136
EcoT14I	CCWWGG	2 cut(s)	1844, 2146
EcoT22I	ATGCAT	1 cut(s)	1774
EcoT38I	GRGCYC	3 cut(s)	789, 952, 1259
ErhI	CCWWGG	2 cut(s)	1844, 2146
Esp3I	CGTCTC	1 cut(s)	190
FalI	AAGNNNNNCTT	2 cut(s)	1125, 1157
FaqI	GGGAC	1 cut(s)	513
FauI	CCCGC	1 cut(s)	115
FbaI	TGATCA	1 cut(s)	940
FriOI	GRGCYC	3 cut(s)	789, 952, 1259
FseI	GGCCGGCC	1 cut(s)	9
FspAI	RTGCGCAY	1 cut(s)	1744
FspBI	CTAG	7 cut(s)	152, 249, 258, 1026, 1568, 1802, 2033
FspI	TGCGCA	1 cut(s)	1744
GlaI	GCGC	4 cut(s)	222, 315, 1744, 1892
GsuI	CTGGAG	1 cut(s)	1844
HaeII	RGCGCY	2 cut(s)	317, 1894
HaeIII	GGCC	8 cut(s)	5, 9, 213, 389, 787, 867, 1162, 1626
HapII	CCGG	8 cut(s)	6, 10, 135, 189, 296, 1038, 1271, 2175
HhaI	GCGC	4 cut(s)	223, 316, 1745, 1893
Hin6I	GCGC	4 cut(s)	221, 314, 1743, 1891
HinP1I	GCGC	4 cut(s)	221, 314, 1743, 1891
HinfI	GANTC	6 cut(s)	167, 266, 1091, 1222, 1231, 1955
HpaII	CCGG	8 cut(s)	6, 10, 135, 189, 296, 1038, 1271, 2175
HphI	GGTGA	8 cut(s)	46, 52, 76, 172, 905, 1541, 1773, 2153
Hpy166II	GTNNAC	3 cut(s)	362, 1060, 1279
Hpy188I	TCNGA	2 cut(s)	1230, 1365
Hpy8I	GTNNAC	3 cut(s)	362, 1060, 1279
Hpy99I	CGWCG	1 cut(s)	164
HpyAV	CCTTC	9 cut(s)	181, 1205, 1243, 1325, 1502, 1616, 1625, 2020, 2225
HpyCH4III	ACNGT	8 cut(s)	52, 184, 478, 521, 1052, 1316, 1663, 1676
HpyCH4IV	ACGT	3 cut(s)	735, 1168, 1689
HpyF3I	CTNAG	7 cut(s)	141, 354, 681, 1376, 1707, 1902, 1951
HpySE526I	ACGT	3 cut(s)	735, 1168, 1689
HspAI	GCGC	4 cut(s)	221, 314, 1743, 1891
Kpn2I	TCCGGA	1 cut(s)	2174
KpnI	GGTACC	1 cut(s)	1038
KroI	GCCGGC	1 cut(s)	5
KroNI	GCCGGC	1 cut(s)	7
Ksp22I	TGATCA	1 cut(s)	940
Kzo9I	GATC	9 cut(s)	112, 591, 753, 916, 940, 1019, 1387, 1531, 1668
LguI	GCTCTTC	1 cut(s)	697
LmnI	GCTCC	2 cut(s)	107, 1475
Lsp1109I	GCAGC	8 cut(s)	55, 303, 500, 598, 762, 1282, 2062, 2144
LweI	GCATC	9 cut(s)	361, 722, 919, 988, 1490, 1759, 1800, 2008, 2059
MabI	ACCWGGT	1 cut(s)	414
MaeI	CTAG	7 cut(s)	152, 249, 258, 1026, 1568, 1802, 2033
MaeII	ACGT	3 cut(s)	735, 1168, 1689
MaeIII	GTNAC	9 cut(s)	52, 736, 1065, 1423, 1606, 1779, 1818, 1984, 2141
MalI	GATC	9 cut(s)	114, 593, 755, 918, 942, 1021, 1389, 1533, 1670
MbiI	CCGCTC	1 cut(s)	210
MboI	GATC	9 cut(s)	112, 591, 753, 916, 940, 1019, 1387, 1531, 1668
MboII	GAAGA	8 cut(s)	553, 714, 1087, 1217, 1421, 1715, 2072, 2197
MflI	RGATCY	1 cut(s)	916
MhlI	GDGCHC	5 cut(s)	353, 789, 952, 1259, 1736
MlyI	GAGTC	2 cut(s)	161, 1100
Mph1103I	ATGCAT	1 cut(s)	1774
MroI	TCCGGA	1 cut(s)	2174
MroNI	GCCGGC	1 cut(s)	5
MspCI	CTTAAG	2 cut(s)	673, 1886
MspI	CCGG	8 cut(s)	6, 10, 135, 189, 296, 1038, 1271, 2175
MspR9I	CCNGG	8 cut(s)	36, 135, 416, 792, 1039, 1164, 1271, 2138
MvaI	CCWGG	5 cut(s)	36, 416, 792, 1164, 2138
MvnI	CGCG	1 cut(s)	954
NaeI	GCCGGC	1 cut(s)	7
NciI	CCSGG	3 cut(s)	135, 1039, 1271
NdeII	GATC	9 cut(s)	112, 591, 753, 916, 940, 1019, 1387, 1531, 1668
NgoMIV	GCCGGC	1 cut(s)	5
NheI	GCTAGC	1 cut(s)	2032
NlaIV	GGNNCC	4 cut(s)	787, 788, 1036, 1477
NmeAIII	GCCGAG	3 cut(s)	958, 1682, 2185
NmuCI	GTSAC	5 cut(s)	52, 736, 1779, 1984, 2141
NsbI	TGCGCA	1 cut(s)	1744
NsiI	ATGCAT	1 cut(s)	1774
NspI	RCATGY	4 cut(s)	248, 764, 977, 2119
NspV	TTCGAA	1 cut(s)	700
PaeI	GCATGC	1 cut(s)	248
PagI	TCATGA	3 cut(s)	420, 1045, 1726
PasI	CCCWGGG	1 cut(s)	2137
PciI	ACATGT	1 cut(s)	2115
PciSI	GCTCTTC	1 cut(s)	697
PcsI	WCGNNNNNNNCGW	2 cut(s)	180, 2064
PdiI	GCCGGC	1 cut(s)	7
PfeI	GAWTC	4 cut(s)	266, 1222, 1231, 1955
Pfl23II	CGTACG	1 cut(s)	1301
PleI	GAGTC	2 cut(s)	161, 1099
PmaCI	CACGTG	1 cut(s)	736
PmlI	CACGTG	1 cut(s)	736
PpsI	GAGTC	2 cut(s)	161, 1099
Ppu21I	YACGTR	1 cut(s)	736
PscI	ACATGT	1 cut(s)	2115
Psp124BI	GAGCTC	1 cut(s)	1259
Psp6I	CCWGG	5 cut(s)	34, 414, 790, 1162, 2136
PspCI	CACGTG	1 cut(s)	736
PspEI	GGTNACC	1 cut(s)	2141
PspGI	CCWGG	5 cut(s)	34, 414, 790, 1162, 2136
PspLI	CGTACG	1 cut(s)	1301
PspN4I	GGNNCC	4 cut(s)	787, 788, 1036, 1477
PspOMI	GGGCCC	1 cut(s)	785
PspPI	GGNCC	7 cut(s)	388, 785, 786, 866, 1625, 1841, 1879
PstI	CTGCAG	2 cut(s)	73, 2080
PsuI	RGATCY	1 cut(s)	916
RigI	GGCCGGCC	1 cut(s)	9
RsaI	GTAC	5 cut(s)	796, 1036, 1303, 1464, 1998
RsaNI	GTAC	5 cut(s)	795, 1035, 1302, 1463, 1997
SacI	GAGCTC	1 cut(s)	1259
SapI	GCTCTTC	1 cut(s)	697
Sau3AI	GATC	9 cut(s)	112, 591, 753, 916, 940, 1019, 1387, 1531, 1668
Sau96I	GGNCC	7 cut(s)	388, 785, 786, 866, 1625, 1841, 1879
SchI	GAGTC	2 cut(s)	161, 1100
ScrFI	CCNGG	8 cut(s)	36, 135, 416, 792, 1039, 1164, 1271, 2138
SduI	GDGCHC	5 cut(s)	353, 789, 952, 1259, 1736
SexAI	ACCWGGT	1 cut(s)	414
SfaNI	GCATC	9 cut(s)	361, 722, 919, 988, 1490, 1759, 1800, 2008, 2059
SfcI	CTRYAG	4 cut(s)	69, 116, 1312, 2076
SfuI	TTCGAA	1 cut(s)	700
SinI	GGWCC	2 cut(s)	1841, 1879
SmlI	CTYRAG	2 cut(s)	673, 1886
SmoI	CTYRAG	2 cut(s)	673, 1886
SpeI	ACTAGT	1 cut(s)	1567
SphI	GCATGC	1 cut(s)	248
SsiI	CCGC	7 cut(s)	108, 210, 241, 293, 954, 1655, 2072
SspMI	CTAG	7 cut(s)	152, 249, 258, 1026, 1568, 1802, 2033
SstI	GAGCTC	1 cut(s)	1259
StyD4I	CCNGG	8 cut(s)	34, 133, 414, 790, 1037, 1162, 1269, 2136
StyI	CCWWGG	2 cut(s)	1844, 2146
TaaI	ACNGT	8 cut(s)	52, 184, 478, 521, 1052, 1316, 1663, 1676
TaiI	ACGT	3 cut(s)	738, 1171, 1692
TaqI	TCGA	7 cut(s)	564, 700, 1534, 1671, 1764, 2067, 2208
TauI	GCSGC	4 cut(s)	213, 244, 1658, 2075
TfiI	GAWTC	4 cut(s)	266, 1222, 1231, 1955
TscAI	CASTG	6 cut(s)	1321, 1407, 1489, 1642, 1681, 2091
TseFI	GTSAC	5 cut(s)	52, 736, 1779, 1984, 2141
TseI	GCWGC	8 cut(s)	68, 316, 488, 611, 775, 1295, 2075, 2132
Tsp45I	GTSAC	5 cut(s)	52, 736, 1779, 1984, 2141
TspDTI	ATGAA	9 cut(s)	547, 1005, 1034, 1161, 1218, 1223, 1262, 1422, 1715
TspGWI	ACGGA	4 cut(s)	148, 265, 914, 2027
TspRI	CASTG	6 cut(s)	1321, 1407, 1489, 1642, 1681, 2091
Vha464I	CTTAAG	2 cut(s)	673, 1886
VpaK11BI	GGWCC	2 cut(s)	1841, 1879
XceI	RCATGY	4 cut(s)	248, 764, 977, 2119
XspI	CTAG	7 cut(s)	152, 249, 258, 1026, 1568, 1802, 2033
Zsp2I	ATGCAT	1 cut(s)	1774

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.