Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0003337

Physical Location & Seq

Genomic Coordinates Reverse (-)

89369595 .. 89376280

Gene Structure 6686 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00068050.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2175 bp

ATGGCTCCGACGACGATGATCGTCACCAACACTCCGGCTCCCGATCTCGCTCTCACCAACCTCGCCTACTGCTCGCCGCTCGATCTCCAGACCTTCGCCATTCCCGGCACCAAGCTCAACCTAGCTCTAATCGCCGACTCGTTCAGCATACAGAAGGGTTACATTGCGCTGAATGCGATTCAACGACGGCATGCTAGGGTTTCTAGCGGTGACTCATTATCTGTGAGCAGGTTTACTCCGCCTGATGATTTCAACCTGACCGTGCTCACTCTGGAATTGGAGTTTGTGAAAAAAGGGACTAAGAGTGAACAGATTGATGCTGTTGCCTTGTCAAGCCAACTTAAAAAGAGGTTTATCAACCAGGTTATGACAGCCGGACAAAGAGTATCATTTGAGTACCTTGGGACTAATTACATTTTTACAGTCAATCAAGCTGTAAGATCCGAAGAGTCTAGCTCTGTTGAAAGAGGGATGATCTCGAGTGAAACATACTTCATTTTTGAGGCACCGAGTGTAAATGGAATAAAGATTGTGAACCAACGTTCTGCTACGACTAGCAATATCTTTAAGCACAAGGAATTCAACCTTCAATCACTTGGCATTGGTGGCCTAGGTGCTGAGTTTGCAGATATATTTCGAAGAGCTTTTGCCTCCCGTGTCTTCCCTGCACATGTTACAAATAAACTAGGGATTAAGCATGTGAAGGGTATGCTGCTTTATGGACCACCTGGTACTGGAAAAACTCTTATGGCGCGTCAAATTGGAAAAATGTTGAATGGGAAAGATCCAAAGATTGTTAATGGTCCAGAAGTCCTGAGCAAGTTCGTTGGTGAAACTGAAAAAAATGTTAGGGATCTATTTGCTGATGCTGAGAATGATCAAAGGCTTCGTGGAGATGAAAGTGAATTGCATGTCATAATCTTTGATGAAATCGATGCTATTTGTAAGTCAAGAGGATCAACTAGAGATGGCACAGGAGTTCATGATAGTATTGTGAACCAGTTACTTACAAAGATTGATGGTGTGGAATCTCTGAATAATGTTTTGCTTATTGGGATGACCAACAGAAAGGATTTGATTGATGAAGCCCTTTTGAGGCCAGGACGTCTGGAAGTTCAAATTGAGATAAGTCTTCCTGATGAAAATGGTCGCTTACAGATTCTTCAAATTCATACGAACAAAATGAAGGAAAGCTCTTTTCTGGCTCCTGATGTCAACCTGCAAGAGCTTGCTGCTCGTACAAAGAACTATAGTGGTGCAGAACTGGAAGGTGTTGTTAAAAGTGCAGTATCATTTGCTTTGAATAGACAACTAAGTTTGGATGATCTTACCAAGCCAGTGGATGAAGAGAGTATTAAGGTTACCATGGATGATTTTCTACGTGCACTCCATGAAATTGTACCTGCATTTGGTGCCTCCACTGATGACCTTGAAAGATGCAGACTTCATGGCATGATGGATTGTGGGGATCGACATAAGCATATCTATCAAAGAGCCATGCTACTTGTGGAACAAGTTAAATCAAGTGAGAGAAGTCCTCTTGTTACATGCCTTTTGGAAGGTCCTAGTGGCAGTGGTAAATCTTCACTAGCAGCTACTGTTGGCATCGACAGTGATTTCCCCTATGTTAAGATTATCTCGGCTGAGGCCATGATTGGTCTTCAAGAGAGCACCAAATGTGCGCAAATCGCAAAGGTTTTTGAAGATGCATATAAGTCACCATTAAGCATTATCATCCTGGACAATATTGAAAGGTTACTGGAGTATATTGCTATTGGTCCGAGGTTTTCAAATTTGATATCTCAAACACTGATGCTCCTTCTCAAGCGCACTCCTCCAAAGGGGAAAAAGCTTCTGGTTTTTGGTACAACAAGTGAATTGGGCTTCTTAGACTCGATTGGTTTTTGTGATACCTTCTCCGTCACTTACAATGTTCCCACATTACAGACACCGGCTGCAAAGAAGGTGCTAGAACAGTTAGATGTCTTTGCTGATGAAGACATAGATGCAGCTGCAGAGGCCCTGAATGATATTCCTATCAAGAAGCTCTATATGGTAATTGAGATGGCAGCCCAGGGTGAGCAAGGAGGGTCTGCCCAGGCAATCTTCTCTGGACGAGCGAAAATAGAGATCAATCACTTTTATGCGATCCTTCAGGATACTGTTGGATATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005794 GO:0005886 GO:0005911 GO:0009506 GO:0012505 GO:0016020 GO:0030054 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04138 ko04721 ko04727 ko04962 map04138 map04721 map04727 map04962

Pfam Domains

Protein Families

AAA CDC48_2 CDC48_N

Protein Analysis

724

Amino Acids

79.51

Weight (kDa)

5.72

Isoelectric Point (pI)

37.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CDC48_2	PF02933	104 - 148	1.1e-06	Cell division protein 48 (CDC48), domain 2
AAA	PF00004	237 - 377	2.5e-36	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	404 - 439	3.3e-10	AAA+ lid domain
AAA	PF00004	518 - 631	2.8e-08	ATPase family associated with various cellular activities (AAA)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003337)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04910
fragaria_vesca	FvH4_3g09180 FvH4_4g01371 FvH4_4g01380
malus_domestica	MD05G1312500.v1.1 MD05G1339600.v1.1 MD10G1294300.v1.1
prunus_persica	Prupe.4G047600_v2.0.a1
pyrus_communis	pycom05g29040 pycom10g24600
rosa_chinensis	RchiOBHm_Chr4g0419821 RchiOBHm_Chr5g0005931 RchiOBHm_Chr5g0005941 RchiOBHm_Chr5g0008441
rosa_laevigata	RLG00000031340 RLG00000031347 RLG00000031352 RLG00000031389 RLG00000031572
rosa_multiflora	Rmu_sc0000720.1_g000006 Rmu_sc0004326.1_g000001 Rmu_sc0006414.1_g000006
rosa_roxburghii	Rroxscaffold_1G00068050
rosa_rugosa	Rorug04G0435800
rosa_samantha	Rh4AG222000 Rh4BG224200 Rh4CG235100 Rh4DG221000 Rh5AG064700 Rh5BG061700 Rh5CG071500 Rh5CG071600 Rh5CG071800 Rh5CG071900
rosa_wichuraiana	Rw4G019100 Rw5G005630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1108
Acc16I	TGCGCA	1 cut(s)	1683
Acc36I	ACCTGC	3 cut(s)	219, 1227, 1411
AccB1I	GGYRCC	3 cut(s)	107, 505, 1412
AccBSI	CCGCTC	1 cut(s)	79
AccII	CGCG	1 cut(s)	754
AciI	CCGC	3 cut(s)	77, 207, 239
AclI	AACGTT	1 cut(s)	541
AclWI	GGATC	6 cut(s)	435, 779, 861, 964, 1476, 2143
AcsI	RAATTY	3 cut(s)	578, 1167, 1792
AcuI	CTGAAG	1 cut(s)	2138
AcyI	GRCGYC	1 cut(s)	1105
AdeI	CACNNNGTG	1 cut(s)	512
AfaI	GTAC	5 cut(s)	398, 733, 1240, 1401, 1867
AfiI	CCNNNNNNNGG	4 cut(s)	734, 1095, 1409, 1841
AflIII	ACRYGT	1 cut(s)	670
AjnI	CCWGG	6 cut(s)	360, 727, 1099, 1737, 2073, 2097
Alw21I	GWGCWC	3 cut(s)	267, 1387, 1673
Alw44I	GTGCAC	1 cut(s)	1383
AlwI	GGATC	6 cut(s)	435, 779, 861, 964, 1476, 2143
AlwNI	CAGNNNCTG	1 cut(s)	1598
Ama87I	CYCGRG	1 cut(s)	478
AoxI	GGCC	4 cut(s)	607, 1097, 1647, 2019
ApaLI	GTGCAC	1 cut(s)	1383
ApeKI	GCWGC	7 cut(s)	712, 1232, 1592, 1955, 2009, 2012, 2069
ApoI	RAATTY	3 cut(s)	578, 1167, 1792
AspA2I	CCTAGG	1 cut(s)	610
AspLEI	GCGC	4 cut(s)	169, 754, 1684, 1830
AspS9I	GGNCC	5 cut(s)	722, 803, 1562, 1778, 2020
AsuC2I	CCSGG	1 cut(s)	105
AsuHPI	GGTGA	6 cut(s)	16, 46, 221, 842, 1710, 2090
AsuII	TTCGAA	1 cut(s)	637
AvaI	CYCGRG	1 cut(s)	478
AvaII	GGWCC	4 cut(s)	722, 803, 1562, 1778
AvrII	CCTAGG	1 cut(s)	610
BaeGI	GKGCMC	1 cut(s)	1387
BanI	GGYRCC	3 cut(s)	107, 505, 1412
BbsI	GAAGAC	4 cut(s)	652, 1124, 1652, 2004
Bbv12I	GWGCWC	3 cut(s)	267, 1387, 1673
BbvCI	CCTCAGC	1 cut(s)	1644
BbvI	GCAGC	7 cut(s)	699, 1219, 1604, 1942, 1999, 2021, 2081
BccI	CCATC	4 cut(s)	962, 1013, 1450, 2059
BceAI	ACGGC	1 cut(s)	203
BciT130I	CCWGG	6 cut(s)	362, 729, 1101, 1739, 2075, 2099
BciVI	GTATCC	1 cut(s)	2152
BclI	TGATCA	1 cut(s)	877
BcnI	CCSGG	1 cut(s)	105
BfmI	CTRYAG	2 cut(s)	1249, 2013
BfuAI	ACCTGC	3 cut(s)	219, 1227, 1411
BfuI	GTATCC	1 cut(s)	2152
BisI	GCNGC	8 cut(s)	77, 713, 1233, 1593, 1956, 2010, 2013, 2070
BlnI	CCTAGG	1 cut(s)	610
BlsI	GCNGC	8 cut(s)	78, 714, 1234, 1594, 1957, 2011, 2014, 2071
Bme1390I	CCNGG	7 cut(s)	105, 362, 729, 1101, 1739, 2075, 2099
Bme18I	GGWCC	4 cut(s)	722, 803, 1562, 1778
BmeT110I	CYCGRG	1 cut(s)	478
BmgT120I	GGNCC	5 cut(s)	722, 803, 1562, 1778, 2020
BmiI	GGNNCC	6 cut(s)	6, 39, 109, 507, 1206, 1414
BmrFI	CCNGG	7 cut(s)	105, 362, 729, 1101, 1739, 2075, 2099
BmsI	GCATC	8 cut(s)	307, 856, 925, 1427, 1614, 1696, 1803, 1996
BpiI	GAAGAC	4 cut(s)	652, 1124, 1652, 2004
BplI	GAGNNNNNCTC	4 cut(s)	440, 472, 1522, 1554
BpmI	CTGGAG	2 cut(s)	71, 1781
Bpu10I	CCTNAGC	2 cut(s)	815, 1644
Bpu14I	TTCGAA	1 cut(s)	637
BpuEI	CTTGAG	1 cut(s)	1808
BpuMI	CCSGG	1 cut(s)	105
Bsa29I	ATCGAT	1 cut(s)	933
BsaAI	YACGTR	1 cut(s)	1382
BsaHI	GRCGYC	1 cut(s)	1105
BsaJI	CCNNGG	7 cut(s)	400, 610, 1365, 1781, 2073, 2074, 2097
BsaXI	ACNNNNNCTCC	8 cut(s)	16, 22, 46, 52, 1371, 1401, 1800, 1830
Bsc4I	CCNNNNNNNGG	4 cut(s)	734, 1095, 1409, 1841
Bse118I	RCCGGY	1 cut(s)	1951
Bse1I	ACTGG	5 cut(s)	739, 1000, 1269, 1337, 1764
Bse3DI	GCAATG	1 cut(s)	162
BseBI	CCWGG	6 cut(s)	362, 729, 1101, 1739, 2075, 2099
BseCI	ATCGAT	1 cut(s)	933
BseDI	CCNNGG	7 cut(s)	400, 610, 1365, 1781, 2073, 2074, 2097
BseGI	GGATG	6 cut(s)	477, 1062, 1327, 1348, 1375, 1734
BseLI	CCNNNNNNNGG	4 cut(s)	734, 1095, 1409, 1841
BseMI	GCAATG	1 cut(s)	162
BseMII	CTCAG	4 cut(s)	609, 806, 861, 1635
BseNI	ACTGG	5 cut(s)	739, 1000, 1269, 1337, 1764
BseRI	GAGGAG	1 cut(s)	1824
BseSI	GKGCMC	1 cut(s)	1387
BseXI	GCAGC	7 cut(s)	699, 1219, 1604, 1942, 1999, 2021, 2081
BsgI	GTGCAG	3 cut(s)	651, 1278, 1305
Bsh1236I	CGCG	1 cut(s)	754
BshFI	GGCC	4 cut(s)	609, 1099, 1649, 2021
BshNI	GGYRCC	3 cut(s)	107, 505, 1412
BshVI	ATCGAT	1 cut(s)	933
BsiHKAI	GWGCWC	3 cut(s)	267, 1387, 1673
BsiHKCI	CYCGRG	1 cut(s)	478
BsiSI	CCGG	4 cut(s)	35, 105, 375, 1952
BslFI	GGGAC	2 cut(s)	310, 418
BslI	CCNNNNNNNGG	4 cut(s)	734, 1095, 1409, 1841
BsmFI	GGGAC	2 cut(s)	310, 418
BsmI	GAATGC	1 cut(s)	178
BsnI	GGCC	4 cut(s)	609, 1099, 1649, 2021
BsoBI	CYCGRG	1 cut(s)	478
Bsp119I	TTCGAA	1 cut(s)	637
Bsp1286I	GDGCHC	3 cut(s)	267, 1387, 1673
Bsp19I	CCATGG	1 cut(s)	1365
BspACI	CCGC	3 cut(s)	77, 207, 239
BspANI	GGCC	4 cut(s)	609, 1099, 1649, 2021
BspCNI	CTCAG	4 cut(s)	610, 807, 862, 1636
BspDI	ATCGAT	1 cut(s)	933
BspFNI	CGCG	1 cut(s)	754
BspHI	TCATGA	1 cut(s)	982
BspLI	GGNNCC	6 cut(s)	6, 39, 109, 507, 1206, 1414
BspMAI	CTGCAG	1 cut(s)	2017
BspMI	ACCTGC	3 cut(s)	219, 1227, 1411
BspPI	GGATC	6 cut(s)	435, 779, 861, 964, 1476, 2143
BspQI	GCTCTTC	1 cut(s)	634
BspT104I	TTCGAA	1 cut(s)	637
BspT107I	GGYRCC	3 cut(s)	107, 505, 1412
BsrBI	CCGCTC	1 cut(s)	79
BsrDI	GCAATG	1 cut(s)	162
BsrFI	RCCGGY	1 cut(s)	1951
BsrI	ACTGG	5 cut(s)	739, 1000, 1269, 1337, 1764
BssAI	RCCGGY	1 cut(s)	1951
BssECI	CCNNGG	7 cut(s)	400, 610, 1365, 1781, 2073, 2074, 2097
BssNI	GRCGYC	1 cut(s)	1105
BssT1I	CCWWGG	3 cut(s)	400, 610, 1365
Bst2UI	CCWGG	6 cut(s)	362, 729, 1101, 1739, 2075, 2099
Bst4CI	ACNGT	6 cut(s)	262, 424, 1600, 1613, 1977, 2164
Bst6I	CTCTTC	3 cut(s)	441, 634, 1341
BstACI	GRCGYC	1 cut(s)	1105
BstAPI	GCANNNNNTGC	1 cut(s)	1412
BstBAI	YACGTR	1 cut(s)	1382
BstBI	TTCGAA	1 cut(s)	637
BstC8I	GCNNGC	3 cut(s)	74, 192, 1230
BstDEI	CTNAG	7 cut(s)	300, 618, 815, 870, 1313, 1644, 1888
BstDSI	CCRYGG	1 cut(s)	1365
BstEII	GGTNACC	1 cut(s)	1360
BstF5I	GGATG	6 cut(s)	477, 1062, 1327, 1348, 1375, 1734
BstFNI	CGCG	1 cut(s)	754
BstHHI	GCGC	4 cut(s)	169, 754, 1684, 1830
BstMWI	GCNNNNNNNGC	7 cut(s)	131, 173, 606, 623, 1412, 1688, 2018
BstNI	CCWGG	6 cut(s)	362, 729, 1101, 1739, 2075, 2099
BstNSI	RCATGY	5 cut(s)	194, 674, 701, 914, 1551
BstPI	GGTNACC	1 cut(s)	1360
BstSCI	CCNGG	7 cut(s)	103, 360, 727, 1099, 1737, 2073, 2097
BstSFI	CTRYAG	2 cut(s)	1249, 2013
BstSLI	GKGCMC	1 cut(s)	1387
BstUI	CGCG	1 cut(s)	754
BstV1I	GCAGC	7 cut(s)	699, 1219, 1604, 1942, 1999, 2021, 2081
BstV2I	GAAGAC	4 cut(s)	652, 1124, 1652, 2004
BstX2I	RGATCY	3 cut(s)	440, 784, 853
BstXI	CCANNNNNNTGG	1 cut(s)	1339
BstYI	RGATCY	3 cut(s)	440, 784, 853
Bsu15I	ATCGAT	1 cut(s)	933
BsuI	GTATCC	1 cut(s)	2152
BsuRI	GGCC	4 cut(s)	609, 1099, 1649, 2021
BsuTUI	ATCGAT	1 cut(s)	933
BtgI	CCRYGG	1 cut(s)	1365
BtsCI	GGATG	6 cut(s)	477, 1062, 1327, 1348, 1375, 1734
BtsI	GCAGTG	1 cut(s)	1579
BtsIMutI	CAGTG	5 cut(s)	1344, 1419, 1579, 1618, 1808
BveI	ACCTGC	3 cut(s)	219, 1227, 1411
Cac8I	GCNNGC	3 cut(s)	74, 192, 1230
CaiI	CAGNNNCTG	1 cut(s)	1598
CciI	TCATGA	1 cut(s)	982
CfoI	GCGC	4 cut(s)	169, 754, 1684, 1830
Cfr10I	RCCGGY	1 cut(s)	1951
Cfr13I	GGNCC	5 cut(s)	722, 803, 1562, 1778, 2020
ClaI	ATCGAT	1 cut(s)	933
CseI	GACGC	1 cut(s)	743
CsiI	ACCWGGT	2 cut(s)	360, 727
Csp6I	GTAC	5 cut(s)	397, 732, 1239, 1400, 1866
CviQI	GTAC	5 cut(s)	397, 732, 1239, 1400, 1866
DdeI	CTNAG	7 cut(s)	300, 618, 815, 870, 1313, 1644, 1888
DraIII	CACNNNGTG	1 cut(s)	512
Eam1104I	CTCTTC	3 cut(s)	441, 634, 1341
EarI	CTCTTC	3 cut(s)	441, 634, 1341
EciI	GGCGGA	1 cut(s)	228
Eco130I	CCWWGG	3 cut(s)	400, 610, 1365
Eco32I	GATATC	1 cut(s)	1800
Eco47I	GGWCC	4 cut(s)	722, 803, 1562, 1778
Eco57I	CTGAAG	1 cut(s)	2138
Eco88I	CYCGRG	1 cut(s)	478
Eco91I	GGTNACC	1 cut(s)	1360
EcoO109I	RGGNCCY	2 cut(s)	1562, 2020
EcoO65I	GGTNACC	1 cut(s)	1360
EcoRI	GAATTC	1 cut(s)	578
EcoRII	CCWGG	6 cut(s)	360, 727, 1099, 1737, 2073, 2097
EcoRV	GATATC	1 cut(s)	1800
EcoT14I	CCWWGG	3 cut(s)	400, 610, 1365
EcoT22I	ATGCAT	1 cut(s)	1711
ErhI	CCWWGG	3 cut(s)	400, 610, 1365
FaqI	GGGAC	2 cut(s)	310, 418
FbaI	TGATCA	1 cut(s)	877
Fnu4HI	GCNGC	8 cut(s)	77, 713, 1233, 1593, 1956, 2010, 2013, 2070
FokI	GGATG	6 cut(s)	484, 1069, 1334, 1355, 1382, 1721
Fsp4HI	GCNGC	8 cut(s)	77, 713, 1233, 1593, 1956, 2010, 2013, 2070
FspI	TGCGCA	1 cut(s)	1683
GlaI	GCGC	4 cut(s)	168, 753, 1683, 1829
GluI	GCNGC	8 cut(s)	77, 713, 1233, 1593, 1956, 2010, 2013, 2070
GsuI	CTGGAG	2 cut(s)	71, 1781
HaeIII	GGCC	4 cut(s)	609, 1099, 1649, 2021
HapII	CCGG	4 cut(s)	35, 105, 375, 1952
HgaI	GACGC	1 cut(s)	743
HhaI	GCGC	4 cut(s)	169, 754, 1684, 1830
Hin1I	GRCGYC	1 cut(s)	1105
Hin6I	GCGC	4 cut(s)	167, 752, 1682, 1828
HinP1I	GCGC	4 cut(s)	167, 752, 1682, 1828
HincII	GTYRAC	1 cut(s)	1216
HindII	GTYRAC	1 cut(s)	1216
HindIII	AAGCTT	1 cut(s)	1850
HinfI	GANTC	7 cut(s)	137, 178, 212, 449, 1028, 1159, 1892
HpaII	CCGG	4 cut(s)	35, 105, 375, 1952
HphI	GGTGA	6 cut(s)	16, 46, 221, 842, 1710, 2090
Hpy166II	GTNNAC	6 cut(s)	234, 308, 535, 997, 1216, 1385
Hpy188I	TCNGA	4 cut(s)	9, 445, 1035, 1782
Hpy8I	GTNNAC	6 cut(s)	234, 308, 535, 997, 1216, 1385
Hpy99I	CGWCG	3 cut(s)	13, 16, 189
HpyCH4III	ACNGT	6 cut(s)	262, 424, 1600, 1613, 1977, 2164
HpyCH4IV	ACGT	3 cut(s)	541, 1105, 1381
HpyF10VI	GCNNNNNNNGC	7 cut(s)	131, 173, 606, 623, 1412, 1688, 2018
HpyF3I	CTNAG	7 cut(s)	300, 618, 815, 870, 1313, 1644, 1888
HpySE526I	ACGT	3 cut(s)	541, 1105, 1381
Hsp92I	GRCGYC	1 cut(s)	1105
HspAI	GCGC	4 cut(s)	167, 752, 1682, 1828
Ksp22I	TGATCA	1 cut(s)	877
LguI	GCTCTTC	1 cut(s)	634
LmnI	GCTCC	4 cut(s)	10, 43, 1210, 1821
Lsp1109I	GCAGC	7 cut(s)	699, 1219, 1604, 1942, 1999, 2021, 2081
LweI	GCATC	8 cut(s)	307, 856, 925, 1427, 1614, 1696, 1803, 1996
MabI	ACCWGGT	2 cut(s)	360, 727
MaeII	ACGT	3 cut(s)	541, 1105, 1381
MbiI	CCGCTC	1 cut(s)	79
MflI	RGATCY	3 cut(s)	440, 784, 853
MhlI	GDGCHC	3 cut(s)	267, 1387, 1673
MlyI	GAGTC	4 cut(s)	131, 206, 458, 1886
MmeI	TCCRAC	2 cut(s)	32, 2146
Mph1103I	ATGCAT	1 cut(s)	1711
MseI	TTAA	9 cut(s)	342, 567, 693, 798, 1278, 1356, 1518, 1629, 1724
MslI	CAYNNNNRTG	1 cut(s)	2142
MspA1I	CMGCKG	1 cut(s)	2012
MspI	CCGG	4 cut(s)	35, 105, 375, 1952
MspR9I	CCNGG	7 cut(s)	105, 362, 729, 1101, 1739, 2075, 2099
Mva1269I	GAATGC	1 cut(s)	178
MvaI	CCWGG	6 cut(s)	362, 729, 1101, 1739, 2075, 2099
MvnI	CGCG	1 cut(s)	754
MwoI	GCNNNNNNNGC	7 cut(s)	131, 173, 606, 623, 1412, 1688, 2018
NciI	CCSGG	1 cut(s)	105
NcoI	CCATGG	1 cut(s)	1365
NlaIV	GGNNCC	6 cut(s)	6, 39, 109, 507, 1206, 1414
NmeAIII	GCCGAG	1 cut(s)	1619
NmuCI	GTSAC	4 cut(s)	22, 209, 1716, 1921
NsbI	TGCGCA	1 cut(s)	1683
NsiI	ATGCAT	1 cut(s)	1711
NspI	RCATGY	5 cut(s)	194, 674, 701, 914, 1551
NspV	TTCGAA	1 cut(s)	637
PaeI	GCATGC	1 cut(s)	194
PaeR7I	CTCGAG	1 cut(s)	478
PagI	TCATGA	1 cut(s)	982
PasI	CCCWGGG	1 cut(s)	2074
PciI	ACATGT	1 cut(s)	670
PciSI	GCTCTTC	1 cut(s)	634
PctI	GAATGC	1 cut(s)	178
PfeI	GAWTC	3 cut(s)	178, 1028, 1159
PfoI	TCCNGGA	1 cut(s)	1737
PkrI	GCNGC	8 cut(s)	78, 714, 1234, 1594, 1957, 2011, 2014, 2071
PleI	GAGTC	4 cut(s)	131, 206, 457, 1886
PpsI	GAGTC	4 cut(s)	131, 206, 457, 1886
Ppu21I	YACGTR	1 cut(s)	1382
PpuMI	RGGWCCY	1 cut(s)	1562
PscI	ACATGT	1 cut(s)	670
Psp1406I	AACGTT	1 cut(s)	541
Psp5II	RGGWCCY	1 cut(s)	1562
Psp6I	CCWGG	6 cut(s)	360, 727, 1099, 1737, 2073, 2097
PspEI	GGTNACC	1 cut(s)	1360
PspGI	CCWGG	6 cut(s)	360, 727, 1099, 1737, 2073, 2097
PspN4I	GGNNCC	6 cut(s)	6, 39, 109, 507, 1206, 1414
PspPI	GGNCC	5 cut(s)	722, 803, 1562, 1778, 2020
PspPPI	RGGWCCY	1 cut(s)	1562
PstI	CTGCAG	1 cut(s)	2017
PstNI	CAGNNNCTG	1 cut(s)	1598
PsuI	RGATCY	3 cut(s)	440, 784, 853
PvuII	CAGCTG	1 cut(s)	2012
RsaI	GTAC	5 cut(s)	398, 733, 1240, 1401, 1867
RsaNI	GTAC	5 cut(s)	397, 732, 1239, 1400, 1866
RseI	CAYNNNNRTG	1 cut(s)	2142
SapI	GCTCTTC	1 cut(s)	634
SaqAI	TTAA	9 cut(s)	342, 567, 693, 798, 1278, 1356, 1518, 1629, 1724
SatI	GCNGC	8 cut(s)	77, 713, 1233, 1593, 1956, 2010, 2013, 2070
Sau96I	GGNCC	5 cut(s)	722, 803, 1562, 1778, 2020
SchI	GAGTC	4 cut(s)	131, 206, 458, 1886
ScrFI	CCNGG	7 cut(s)	105, 362, 729, 1101, 1739, 2075, 2099
SduI	GDGCHC	3 cut(s)	267, 1387, 1673
SexAI	ACCWGGT	2 cut(s)	360, 727
SfaNI	GCATC	8 cut(s)	307, 856, 925, 1427, 1614, 1696, 1803, 1996
SfcI	CTRYAG	2 cut(s)	1249, 2013
Sfr274I	CTCGAG	1 cut(s)	478
SfuI	TTCGAA	1 cut(s)	637
SinI	GGWCC	4 cut(s)	722, 803, 1562, 1778
SlaI	CTCGAG	1 cut(s)	478
SmiMI	CAYNNNNRTG	1 cut(s)	2142
SmlI	CTYRAG	2 cut(s)	478, 1823
SmoI	CTYRAG	2 cut(s)	478, 1823
SphI	GCATGC	1 cut(s)	194
SsiI	CCGC	3 cut(s)	77, 207, 239
SspI	AATATT	1 cut(s)	1747
StyD4I	CCNGG	7 cut(s)	103, 360, 727, 1099, 1737, 2073, 2097
StyI	CCWWGG	3 cut(s)	400, 610, 1365
TaaI	ACNGT	6 cut(s)	262, 424, 1600, 1613, 1977, 2164
TaiI	ACGT	3 cut(s)	544, 1108, 1384
TaqI	TCGA	7 cut(s)	81, 479, 637, 933, 1471, 1608, 1895
TauI	GCSGC	1 cut(s)	79
TfiI	GAWTC	3 cut(s)	178, 1028, 1159
Tru1I	TTAA	9 cut(s)	342, 567, 693, 798, 1278, 1356, 1518, 1629, 1724
Tru9I	TTAA	9 cut(s)	342, 567, 693, 798, 1278, 1356, 1518, 1629, 1724
TscAI	CASTG	5 cut(s)	1344, 1426, 1579, 1618, 1815
TseFI	GTSAC	4 cut(s)	22, 209, 1716, 1921
TseI	GCWGC	7 cut(s)	712, 1232, 1592, 1955, 2009, 2012, 2069
Tsp45I	GTSAC	4 cut(s)	22, 209, 1716, 1921
TspGWI	ACGGA	1 cut(s)	1909
TspRI	CASTG	5 cut(s)	1344, 1426, 1579, 1618, 1815
VneI	GTGCAC	1 cut(s)	1383
VpaK11BI	GGWCC	4 cut(s)	722, 803, 1562, 1778
XapI	RAATTY	3 cut(s)	578, 1167, 1792
XceI	RCATGY	5 cut(s)	194, 674, 701, 914, 1551
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1504
XhoI	CTCGAG	1 cut(s)	478
XmaJI	CCTAGG	1 cut(s)	610
ZraI	GACGTC	1 cut(s)	1106
Zsp2I	ATGCAT	1 cut(s)	1711

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.