Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0003337

Physical Location & Seq

Genomic Coordinates Forward (+)

5080827 .. 5087502

Gene Structure 6676 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG061700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2199 bp

ATGGCTCCGACGACGATGATCGTCACCAACACTCCGGCCCCCGACCTCGCTCTCACCAACCTCGCCTACTGCTCGCCGCTCGATCTCCAGACCTTCGCCATTCCCGGCACCAAGCTCAACCTAGCTCTAATCGCCGACTCGTTCGTCCTCTCAGTCTGTCCTCACCAGAGCATACAGAAGGGTTACATTGCGCTGAATGCGATTCAACGACGGCATGCTAGGGTTTCTAGCGGTGACTCATTATCTGTGAGCAGGTTTACTCCGCCTGATGATTTCAACCTGACCGTGCTCACTCTGGAATTGGAGTTTGTGAAAAAAGGGACTAAGAGTGAACAGATTGATGCTGTTGCCTTGTCGAGCCAACTTCAAAAGAGGTTTATCAACCAGGTTATGACAGCCGGACAAAGAGTATCATTTGAGTACCTTGGGACTAATTACATTTTTACAGTCAATCAAGCTGTAAGATCCGAAGAGGCTAGCTCTGTTGAAAGAGGGATGATCTCGAGTGAAACATACTTCATTTTTGAGGCACCGAGTGGAAATGGAATAAAGATTGTGAACCAACGTTCTGCTACGACTAGCAATATCTTTAAGCACAAGGAATTCAACCTTCAATCACTTGGCATTGGTGGCCTAGGTGCTGAGTTTGCAGATATATTTCGAAGAGCTTTTGCCTCCCGTGTCTTCCCTGCACATGTTACAAATAAACTAGGGATTAAGCATGTGAAGGGTATGCTGCTTTATGGACCACCTGGTACTGGAAAAACTCTTATGGCGCGTCAAATTGGAAAAATGTTGAATGGGAAAGATCCAAAGATTGTTAATGGTCCAGAAGTCCTGAGCAAGTTCGTTGGTGAAACTGAAAAAAATGTTAGGGATCTATTTGCTGATGCTGAGAATGATCAAAGGCTTCGTGGAGATGAAAGTGAATTGCATGTCATAATCTTTGATGAAATCGATGCTATTTGTAAGTCAAGAGGATCAACTAGAGATGGCACAGGAGTTCATGATAGTATTGTGAACCAGTTACTTACAAAGATTGATGGTGTGGAATCTCTGAATAATGTTTTGCTTATTGGGATGACCAACAGAAAGGATTTGATTGATGAAGCCCTTTTGAGGCCAGGACGTCTGGAAGTTCAAATTGAGATAAGTCTTCCTGATGAAAATGGTCGCATACAGATTCTTCAAATTCATACGAACAAAATGAAGGAAAGCTCTTTTCTTGCTCCTGATGTCAACCTGCAAGAGCTTGCTGCTCGTACAAAGAACTATAGTGGTGCAGAACTGGAAGGTGTTGTTAAAAGTGCAGTATCATTTGCTTTGAATAGACAACTAAGTTTGGAGGATCTTACCAAGCCAGTGGATGAAGAGAGTATTAAGGTTACCATGGATGGTTTTCTACGTGCACTCCATGAAATTGTACCTGCATTTGGTGCCTCCACTGATGACCTTGAAAGATGCAGACTTCATGGCATGGTGTATTGTGGGGATCGACATAAGCATATCTATCAAAGAGCCATGCTACTTGTGGAACAAGTTAAATCAAGTGAGAGAAGTCCTCTTGTTACATGCCTTTTGGAAGGTCCTAGTGGCAGTGGTAAATCTTCACTAGCAGCTACTGTTGGCATCGACAGTGATTTCCCCTATGTTAAGATTATCTCGGCTGAGGCCATGATTGGTCTTCAAGAGAGCACCAAATGTGCGCAAATCGCAAAGGTTTTTGAAGATGCATACAAGTCACCATTAAGCATTATCATCCTGGACAATATTGAAAGGTTACTGGAGTATATTGCTATTGGTCCGAGGTTTTCAAATTTGATATCTCAAACACTGATGCTCCTTCTCAAGCGCACTCCTCCAAAGGGGAAAAAGCTTCTGGTTTTTGGTACAACAAGTGAATTGGGCTTCTTAGACTCGATTGGTTTTTGTGATACCTTCTCCGTCACTTACAATGTTCCCACATTACAGACACCGGCTGCAAAGAAGGTGCTAGAACAGTTAAATGTCTTTGCTGATGAAGACATAGATGCAGCTGCAGAGGCCCTGAATGATATTCCTATCAAGAAGCTCTATATGGTAATTGAGATGGCAGCCCAGGGTGAGCAAGGAGGGTCTGCCCAGGCAATCTTCTCTGGACAAGCGAAAATAGAGATCAATCACTTTTATGCGATCCTTCAGGATACTGTTAGATATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005794 GO:0005886 GO:0005911 GO:0009506 GO:0012505 GO:0016020 GO:0030054 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04138 ko04721 ko04727 ko04962 map04138 map04721 map04727 map04962

Pfam Domains

Protein Families

AAA CDC48_2 CDC48_N

Protein Analysis

732

Amino Acids

80.36

Weight (kDa)

5.91

Isoelectric Point (pI)

38.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CDC48_2	PF02933	112 - 156	5.8e-06	Cell division protein 48 (CDC48), domain 2
AAA	PF00004	245 - 385	2.5e-36	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	412 - 447	3.8e-10	AAA+ lid domain
AAA	PF00004	526 - 639	2.9e-08	ATPase family associated with various cellular activities (AAA)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003337)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04910
fragaria_vesca	FvH4_3g09180 FvH4_4g01371 FvH4_4g01380
malus_domestica	MD05G1312500.v1.1 MD05G1339600.v1.1 MD10G1294300.v1.1
prunus_persica	Prupe.4G047600_v2.0.a1
pyrus_communis	pycom05g29040 pycom10g24600
rosa_chinensis	RchiOBHm_Chr4g0419821 RchiOBHm_Chr5g0005931 RchiOBHm_Chr5g0005941 RchiOBHm_Chr5g0008441
rosa_laevigata	RLG00000031340 RLG00000031347 RLG00000031352 RLG00000031389 RLG00000031572
rosa_multiflora	Rmu_sc0000720.1_g000006 Rmu_sc0004326.1_g000001 Rmu_sc0006414.1_g000006
rosa_roxburghii	Rroxscaffold_1G00068050
rosa_rugosa	Rorug04G0435800
rosa_samantha	Rh4AG222000 Rh4BG224200 Rh4CG235100 Rh4DG221000 Rh5AG064700 Rh5BG061700 Rh5CG071500 Rh5CG071600 Rh5CG071800 Rh5CG071900
rosa_wichuraiana	Rw4G019100 Rw5G005630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	143
AatII	GACGTC	1 cut(s)	1132
Acc16I	TGCGCA	1 cut(s)	1707
Acc36I	ACCTGC	3 cut(s)	243, 1251, 1435
AccB1I	GGYRCC	3 cut(s)	107, 529, 1436
AccBSI	CCGCTC	1 cut(s)	79
AccII	CGCG	1 cut(s)	778
AciI	CCGC	3 cut(s)	77, 231, 263
AclI	AACGTT	1 cut(s)	565
AclWI	GGATC	7 cut(s)	459, 803, 885, 988, 1356, 1500, 2167
AcsI	RAATTY	3 cut(s)	602, 1191, 1816
AcuI	CTGAAG	1 cut(s)	2162
AcyI	GRCGYC	1 cut(s)	1129
AdeI	CACNNNGTG	1 cut(s)	536
AfaI	GTAC	5 cut(s)	422, 757, 1264, 1425, 1891
AfiI	CCNNNNNNNGG	4 cut(s)	758, 1119, 1433, 1865
AflIII	ACRYGT	1 cut(s)	694
AjnI	CCWGG	6 cut(s)	384, 751, 1123, 1761, 2097, 2121
Alw21I	GWGCWC	3 cut(s)	291, 1411, 1697
Alw44I	GTGCAC	1 cut(s)	1407
AlwI	GGATC	7 cut(s)	459, 803, 885, 988, 1356, 1500, 2167
AlwNI	CAGNNNCTG	1 cut(s)	1622
Ama87I	CYCGRG	1 cut(s)	502
AoxI	GGCC	5 cut(s)	36, 631, 1121, 1671, 2043
ApaLI	GTGCAC	1 cut(s)	1407
ApeKI	GCWGC	7 cut(s)	736, 1256, 1616, 1979, 2033, 2036, 2093
ApoI	RAATTY	3 cut(s)	602, 1191, 1816
AspA2I	CCTAGG	1 cut(s)	634
AspLEI	GCGC	4 cut(s)	193, 778, 1708, 1854
AspS9I	GGNCC	6 cut(s)	37, 746, 827, 1586, 1802, 2044
AsuC2I	CCSGG	1 cut(s)	105
AsuHPI	GGTGA	7 cut(s)	16, 46, 155, 245, 866, 1734, 2114
AsuII	TTCGAA	1 cut(s)	661
AsuNHI	GCTAGC	1 cut(s)	476
AvaI	CYCGRG	1 cut(s)	502
AvaII	GGWCC	4 cut(s)	746, 827, 1586, 1802
AvrII	CCTAGG	1 cut(s)	634
BaeGI	GKGCMC	1 cut(s)	1411
BanI	GGYRCC	3 cut(s)	107, 529, 1436
BbsI	GAAGAC	4 cut(s)	676, 1148, 1676, 2028
Bbv12I	GWGCWC	3 cut(s)	291, 1411, 1697
BbvCI	CCTCAGC	1 cut(s)	1668
BbvI	GCAGC	7 cut(s)	723, 1243, 1628, 1966, 2023, 2045, 2105
BccI	CCATC	4 cut(s)	986, 1037, 1388, 2083
BceAI	ACGGC	1 cut(s)	227
BciT130I	CCWGG	6 cut(s)	386, 753, 1125, 1763, 2099, 2123
BciVI	GTATCC	1 cut(s)	2176
BclI	TGATCA	1 cut(s)	901
BcnI	CCSGG	1 cut(s)	105
BfmI	CTRYAG	2 cut(s)	1273, 2037
BfuAI	ACCTGC	3 cut(s)	243, 1251, 1435
BfuI	GTATCC	1 cut(s)	2176
BisI	GCNGC	8 cut(s)	77, 737, 1257, 1617, 1980, 2034, 2037, 2094
BlnI	CCTAGG	1 cut(s)	634
BlsI	GCNGC	8 cut(s)	78, 738, 1258, 1618, 1981, 2035, 2038, 2095
Bme1390I	CCNGG	7 cut(s)	105, 386, 753, 1125, 1763, 2099, 2123
Bme18I	GGWCC	4 cut(s)	746, 827, 1586, 1802
BmeT110I	CYCGRG	1 cut(s)	502
BmgT120I	GGNCC	6 cut(s)	37, 746, 827, 1586, 1802, 2044
BmiI	GGNNCC	5 cut(s)	6, 39, 109, 531, 1438
BmrFI	CCNGG	7 cut(s)	105, 386, 753, 1125, 1763, 2099, 2123
BmsI	GCATC	8 cut(s)	331, 880, 949, 1451, 1638, 1720, 1827, 2020
BmtI	GCTAGC	1 cut(s)	480
BpiI	GAAGAC	4 cut(s)	676, 1148, 1676, 2028
BplI	GAGNNNNNCTC	4 cut(s)	464, 496, 1546, 1578
BpmI	CTGGAG	2 cut(s)	71, 1805
Bpu10I	CCTNAGC	2 cut(s)	839, 1668
Bpu14I	TTCGAA	1 cut(s)	661
BpuEI	CTTGAG	1 cut(s)	1832
BpuMI	CCSGG	1 cut(s)	105
Bsa29I	ATCGAT	1 cut(s)	957
BsaAI	YACGTR	1 cut(s)	1406
BsaHI	GRCGYC	1 cut(s)	1129
BsaJI	CCNNGG	7 cut(s)	424, 634, 1389, 1805, 2097, 2098, 2121
BsaXI	ACNNNNNCTCC	6 cut(s)	16, 46, 1395, 1425, 1824, 1854
Bsc4I	CCNNNNNNNGG	4 cut(s)	758, 1119, 1433, 1865
Bse118I	RCCGGY	1 cut(s)	1975
Bse1I	ACTGG	5 cut(s)	763, 1024, 1293, 1361, 1788
Bse3DI	GCAATG	1 cut(s)	186
BseBI	CCWGG	6 cut(s)	386, 753, 1125, 1763, 2099, 2123
BseCI	ATCGAT	1 cut(s)	957
BseDI	CCNNGG	7 cut(s)	424, 634, 1389, 1805, 2097, 2098, 2121
BseGI	GGATG	5 cut(s)	501, 1086, 1372, 1399, 1758
BseLI	CCNNNNNNNGG	4 cut(s)	758, 1119, 1433, 1865
BseMI	GCAATG	1 cut(s)	186
BseMII	CTCAG	5 cut(s)	165, 633, 830, 885, 1659
BseNI	ACTGG	5 cut(s)	763, 1024, 1293, 1361, 1788
BseRI	GAGGAG	1 cut(s)	1848
BseSI	GKGCMC	1 cut(s)	1411
BseXI	GCAGC	7 cut(s)	723, 1243, 1628, 1966, 2023, 2045, 2105
BsgI	GTGCAG	3 cut(s)	675, 1302, 1329
Bsh1236I	CGCG	1 cut(s)	778
BshFI	GGCC	5 cut(s)	38, 633, 1123, 1673, 2045
BshNI	GGYRCC	3 cut(s)	107, 529, 1436
BshVI	ATCGAT	1 cut(s)	957
BsiHKAI	GWGCWC	3 cut(s)	291, 1411, 1697
BsiHKCI	CYCGRG	1 cut(s)	502
BsiSI	CCGG	4 cut(s)	35, 105, 399, 1976
BslFI	GGGAC	2 cut(s)	334, 442
BslI	CCNNNNNNNGG	4 cut(s)	758, 1119, 1433, 1865
BsmFI	GGGAC	2 cut(s)	334, 442
BsmI	GAATGC	1 cut(s)	202
BsnI	GGCC	5 cut(s)	38, 633, 1123, 1673, 2045
BsoBI	CYCGRG	1 cut(s)	502
Bsp119I	TTCGAA	1 cut(s)	661
Bsp1286I	GDGCHC	3 cut(s)	291, 1411, 1697
Bsp19I	CCATGG	1 cut(s)	1389
BspACI	CCGC	3 cut(s)	77, 231, 263
BspANI	GGCC	5 cut(s)	38, 633, 1123, 1673, 2045
BspCNI	CTCAG	5 cut(s)	164, 634, 831, 886, 1660
BspDI	ATCGAT	1 cut(s)	957
BspFNI	CGCG	1 cut(s)	778
BspHI	TCATGA	1 cut(s)	1006
BspLI	GGNNCC	5 cut(s)	6, 39, 109, 531, 1438
BspMAI	CTGCAG	1 cut(s)	2041
BspMI	ACCTGC	3 cut(s)	243, 1251, 1435
BspOI	GCTAGC	1 cut(s)	480
BspPI	GGATC	7 cut(s)	459, 803, 885, 988, 1356, 1500, 2167
BspQI	GCTCTTC	1 cut(s)	658
BspT104I	TTCGAA	1 cut(s)	661
BspT107I	GGYRCC	3 cut(s)	107, 529, 1436
BsrBI	CCGCTC	1 cut(s)	79
BsrDI	GCAATG	1 cut(s)	186
BsrFI	RCCGGY	1 cut(s)	1975
BsrI	ACTGG	5 cut(s)	763, 1024, 1293, 1361, 1788
BssAI	RCCGGY	1 cut(s)	1975
BssECI	CCNNGG	7 cut(s)	424, 634, 1389, 1805, 2097, 2098, 2121
BssNI	GRCGYC	1 cut(s)	1129
BssT1I	CCWWGG	3 cut(s)	424, 634, 1389
Bst2UI	CCWGG	6 cut(s)	386, 753, 1125, 1763, 2099, 2123
Bst4CI	ACNGT	6 cut(s)	286, 448, 1624, 1637, 2001, 2188
Bst6I	CTCTTC	3 cut(s)	465, 658, 1365
BstACI	GRCGYC	1 cut(s)	1129
BstAPI	GCANNNNNTGC	1 cut(s)	1436
BstBAI	YACGTR	1 cut(s)	1406
BstBI	TTCGAA	1 cut(s)	661
BstC8I	GCNNGC	4 cut(s)	74, 216, 478, 1254
BstDEI	CTNAG	8 cut(s)	151, 324, 642, 839, 894, 1337, 1668, 1912
BstDSI	CCRYGG	1 cut(s)	1389
BstEII	GGTNACC	1 cut(s)	1384
BstF5I	GGATG	5 cut(s)	501, 1086, 1372, 1399, 1758
BstFNI	CGCG	1 cut(s)	778
BstHHI	GCGC	4 cut(s)	193, 778, 1708, 1854
BstMWI	GCNNNNNNNGC	7 cut(s)	131, 197, 630, 647, 1436, 1712, 2042
BstNI	CCWGG	6 cut(s)	386, 753, 1125, 1763, 2099, 2123
BstNSI	RCATGY	5 cut(s)	218, 698, 725, 938, 1575
BstPI	GGTNACC	1 cut(s)	1384
BstSCI	CCNGG	7 cut(s)	103, 384, 751, 1123, 1761, 2097, 2121
BstSFI	CTRYAG	2 cut(s)	1273, 2037
BstSLI	GKGCMC	1 cut(s)	1411
BstUI	CGCG	1 cut(s)	778
BstV1I	GCAGC	7 cut(s)	723, 1243, 1628, 1966, 2023, 2045, 2105
BstV2I	GAAGAC	4 cut(s)	676, 1148, 1676, 2028
BstX2I	RGATCY	4 cut(s)	464, 808, 877, 1348
BstXI	CCANNNNNNTGG	1 cut(s)	1363
BstYI	RGATCY	4 cut(s)	464, 808, 877, 1348
Bsu15I	ATCGAT	1 cut(s)	957
BsuI	GTATCC	1 cut(s)	2176
BsuRI	GGCC	5 cut(s)	38, 633, 1123, 1673, 2045
BsuTUI	ATCGAT	1 cut(s)	957
BtgI	CCRYGG	1 cut(s)	1389
BtsCI	GGATG	5 cut(s)	501, 1086, 1372, 1399, 1758
BtsI	GCAGTG	1 cut(s)	1603
BtsIMutI	CAGTG	5 cut(s)	1368, 1443, 1603, 1642, 1832
BveI	ACCTGC	3 cut(s)	243, 1251, 1435
Cac8I	GCNNGC	4 cut(s)	74, 216, 478, 1254
CaiI	CAGNNNCTG	1 cut(s)	1622
CciI	TCATGA	1 cut(s)	1006
CfoI	GCGC	4 cut(s)	193, 778, 1708, 1854
Cfr10I	RCCGGY	1 cut(s)	1975
Cfr13I	GGNCC	6 cut(s)	37, 746, 827, 1586, 1802, 2044
ClaI	ATCGAT	1 cut(s)	957
CseI	GACGC	1 cut(s)	767
CsiI	ACCWGGT	2 cut(s)	384, 751
Csp6I	GTAC	5 cut(s)	421, 756, 1263, 1424, 1890
CviQI	GTAC	5 cut(s)	421, 756, 1263, 1424, 1890
DdeI	CTNAG	8 cut(s)	151, 324, 642, 839, 894, 1337, 1668, 1912
DraIII	CACNNNGTG	1 cut(s)	536
DrdI	GACNNNNNNGTC	1 cut(s)	143
DseDI	GACNNNNNNGTC	1 cut(s)	143
Eam1104I	CTCTTC	3 cut(s)	465, 658, 1365
EarI	CTCTTC	3 cut(s)	465, 658, 1365
EciI	GGCGGA	1 cut(s)	252
Eco130I	CCWWGG	3 cut(s)	424, 634, 1389
Eco32I	GATATC	1 cut(s)	1824
Eco47I	GGWCC	4 cut(s)	746, 827, 1586, 1802
Eco57I	CTGAAG	1 cut(s)	2162
Eco88I	CYCGRG	1 cut(s)	502
Eco91I	GGTNACC	1 cut(s)	1384
EcoO109I	RGGNCCY	2 cut(s)	1586, 2044
EcoO65I	GGTNACC	1 cut(s)	1384
EcoRI	GAATTC	1 cut(s)	602
EcoRII	CCWGG	6 cut(s)	384, 751, 1123, 1761, 2097, 2121
EcoRV	GATATC	1 cut(s)	1824
EcoT14I	CCWWGG	3 cut(s)	424, 634, 1389
EcoT22I	ATGCAT	1 cut(s)	1735
ErhI	CCWWGG	3 cut(s)	424, 634, 1389
FaqI	GGGAC	2 cut(s)	334, 442
FbaI	TGATCA	1 cut(s)	901
Fnu4HI	GCNGC	8 cut(s)	77, 737, 1257, 1617, 1980, 2034, 2037, 2094
FokI	GGATG	5 cut(s)	508, 1093, 1379, 1406, 1745
Fsp4HI	GCNGC	8 cut(s)	77, 737, 1257, 1617, 1980, 2034, 2037, 2094
FspI	TGCGCA	1 cut(s)	1707
GlaI	GCGC	4 cut(s)	192, 777, 1707, 1853
GluI	GCNGC	8 cut(s)	77, 737, 1257, 1617, 1980, 2034, 2037, 2094
GsuI	CTGGAG	2 cut(s)	71, 1805
HaeIII	GGCC	5 cut(s)	38, 633, 1123, 1673, 2045
HapII	CCGG	4 cut(s)	35, 105, 399, 1976
HgaI	GACGC	1 cut(s)	767
HhaI	GCGC	4 cut(s)	193, 778, 1708, 1854
Hin1I	GRCGYC	1 cut(s)	1129
Hin6I	GCGC	4 cut(s)	191, 776, 1706, 1852
HinP1I	GCGC	4 cut(s)	191, 776, 1706, 1852
HincII	GTYRAC	1 cut(s)	1240
HindII	GTYRAC	1 cut(s)	1240
HindIII	AAGCTT	1 cut(s)	1874
HinfI	GANTC	6 cut(s)	137, 202, 236, 1052, 1183, 1916
HpaII	CCGG	4 cut(s)	35, 105, 399, 1976
HphI	GGTGA	7 cut(s)	16, 46, 155, 245, 866, 1734, 2114
Hpy166II	GTNNAC	6 cut(s)	258, 332, 559, 1021, 1240, 1409
Hpy188I	TCNGA	4 cut(s)	9, 469, 1059, 1806
Hpy8I	GTNNAC	6 cut(s)	258, 332, 559, 1021, 1240, 1409
Hpy99I	CGWCG	3 cut(s)	13, 16, 213
HpyCH4III	ACNGT	6 cut(s)	286, 448, 1624, 1637, 2001, 2188
HpyCH4IV	ACGT	3 cut(s)	565, 1129, 1405
HpyF10VI	GCNNNNNNNGC	7 cut(s)	131, 197, 630, 647, 1436, 1712, 2042
HpyF3I	CTNAG	8 cut(s)	151, 324, 642, 839, 894, 1337, 1668, 1912
HpySE526I	ACGT	3 cut(s)	565, 1129, 1405
Hsp92I	GRCGYC	1 cut(s)	1129
HspAI	GCGC	4 cut(s)	191, 776, 1706, 1852
Ksp22I	TGATCA	1 cut(s)	901
LguI	GCTCTTC	1 cut(s)	658
LmnI	GCTCC	3 cut(s)	10, 1234, 1845
Lsp1109I	GCAGC	7 cut(s)	723, 1243, 1628, 1966, 2023, 2045, 2105
LweI	GCATC	8 cut(s)	331, 880, 949, 1451, 1638, 1720, 1827, 2020
MabI	ACCWGGT	2 cut(s)	384, 751
MaeII	ACGT	3 cut(s)	565, 1129, 1405
MbiI	CCGCTC	1 cut(s)	79
MflI	RGATCY	4 cut(s)	464, 808, 877, 1348
MhlI	GDGCHC	3 cut(s)	291, 1411, 1697
MlyI	GAGTC	3 cut(s)	131, 230, 1910
MmeI	TCCRAC	1 cut(s)	32
Mph1103I	ATGCAT	1 cut(s)	1735
MseI	TTAA	9 cut(s)	591, 717, 822, 1302, 1380, 1542, 1653, 1748, 2003
MslI	CAYNNNNRTG	1 cut(s)	2166
MspA1I	CMGCKG	1 cut(s)	2036
MspI	CCGG	4 cut(s)	35, 105, 399, 1976
MspR9I	CCNGG	7 cut(s)	105, 386, 753, 1125, 1763, 2099, 2123
Mva1269I	GAATGC	1 cut(s)	202
MvaI	CCWGG	6 cut(s)	386, 753, 1125, 1763, 2099, 2123
MvnI	CGCG	1 cut(s)	778
MwoI	GCNNNNNNNGC	7 cut(s)	131, 197, 630, 647, 1436, 1712, 2042
NciI	CCSGG	1 cut(s)	105
NcoI	CCATGG	1 cut(s)	1389
NheI	GCTAGC	1 cut(s)	476
NlaIV	GGNNCC	5 cut(s)	6, 39, 109, 531, 1438
NmeAIII	GCCGAG	1 cut(s)	1643
NmuCI	GTSAC	4 cut(s)	22, 233, 1740, 1945
NsbI	TGCGCA	1 cut(s)	1707
NsiI	ATGCAT	1 cut(s)	1735
NspI	RCATGY	5 cut(s)	218, 698, 725, 938, 1575
NspV	TTCGAA	1 cut(s)	661
PaeI	GCATGC	1 cut(s)	218
PaeR7I	CTCGAG	1 cut(s)	502
PagI	TCATGA	1 cut(s)	1006
PasI	CCCWGGG	1 cut(s)	2098
PciI	ACATGT	1 cut(s)	694
PciSI	GCTCTTC	1 cut(s)	658
PctI	GAATGC	1 cut(s)	202
PfeI	GAWTC	3 cut(s)	202, 1052, 1183
PfoI	TCCNGGA	1 cut(s)	1761
PkrI	GCNGC	8 cut(s)	78, 738, 1258, 1618, 1981, 2035, 2038, 2095
PleI	GAGTC	3 cut(s)	131, 230, 1910
PpsI	GAGTC	3 cut(s)	131, 230, 1910
Ppu21I	YACGTR	1 cut(s)	1406
PpuMI	RGGWCCY	1 cut(s)	1586
PscI	ACATGT	1 cut(s)	694
Psp1406I	AACGTT	1 cut(s)	565
Psp5II	RGGWCCY	1 cut(s)	1586
Psp6I	CCWGG	6 cut(s)	384, 751, 1123, 1761, 2097, 2121
PspEI	GGTNACC	1 cut(s)	1384
PspGI	CCWGG	6 cut(s)	384, 751, 1123, 1761, 2097, 2121
PspN4I	GGNNCC	5 cut(s)	6, 39, 109, 531, 1438
PspPI	GGNCC	6 cut(s)	37, 746, 827, 1586, 1802, 2044
PspPPI	RGGWCCY	1 cut(s)	1586
PstI	CTGCAG	1 cut(s)	2041
PstNI	CAGNNNCTG	1 cut(s)	1622
PsuI	RGATCY	4 cut(s)	464, 808, 877, 1348
PvuII	CAGCTG	1 cut(s)	2036
RsaI	GTAC	5 cut(s)	422, 757, 1264, 1425, 1891
RsaNI	GTAC	5 cut(s)	421, 756, 1263, 1424, 1890
RseI	CAYNNNNRTG	1 cut(s)	2166
SapI	GCTCTTC	1 cut(s)	658
SaqAI	TTAA	9 cut(s)	591, 717, 822, 1302, 1380, 1542, 1653, 1748, 2003
SatI	GCNGC	8 cut(s)	77, 737, 1257, 1617, 1980, 2034, 2037, 2094
Sau96I	GGNCC	6 cut(s)	37, 746, 827, 1586, 1802, 2044
SchI	GAGTC	3 cut(s)	131, 230, 1910
ScrFI	CCNGG	7 cut(s)	105, 386, 753, 1125, 1763, 2099, 2123
SduI	GDGCHC	3 cut(s)	291, 1411, 1697
SexAI	ACCWGGT	2 cut(s)	384, 751
SfaNI	GCATC	8 cut(s)	331, 880, 949, 1451, 1638, 1720, 1827, 2020
SfcI	CTRYAG	2 cut(s)	1273, 2037
Sfr274I	CTCGAG	1 cut(s)	502
SfuI	TTCGAA	1 cut(s)	661
SinI	GGWCC	4 cut(s)	746, 827, 1586, 1802
SlaI	CTCGAG	1 cut(s)	502
SmiMI	CAYNNNNRTG	1 cut(s)	2166
SmlI	CTYRAG	2 cut(s)	502, 1847
SmoI	CTYRAG	2 cut(s)	502, 1847
SphI	GCATGC	1 cut(s)	218
SsiI	CCGC	3 cut(s)	77, 231, 263
SspI	AATATT	1 cut(s)	1771
StyD4I	CCNGG	7 cut(s)	103, 384, 751, 1123, 1761, 2097, 2121
StyI	CCWWGG	3 cut(s)	424, 634, 1389
TaaI	ACNGT	6 cut(s)	286, 448, 1624, 1637, 2001, 2188
TaiI	ACGT	3 cut(s)	568, 1132, 1408
TaqI	TCGA	8 cut(s)	81, 356, 503, 661, 957, 1495, 1632, 1919
TauI	GCSGC	1 cut(s)	79
TfiI	GAWTC	3 cut(s)	202, 1052, 1183
Tru1I	TTAA	9 cut(s)	591, 717, 822, 1302, 1380, 1542, 1653, 1748, 2003
Tru9I	TTAA	9 cut(s)	591, 717, 822, 1302, 1380, 1542, 1653, 1748, 2003
TscAI	CASTG	5 cut(s)	1368, 1450, 1603, 1642, 1839
TseFI	GTSAC	4 cut(s)	22, 233, 1740, 1945
TseI	GCWGC	7 cut(s)	736, 1256, 1616, 1979, 2033, 2036, 2093
Tsp45I	GTSAC	4 cut(s)	22, 233, 1740, 1945
TspGWI	ACGGA	1 cut(s)	1933
TspRI	CASTG	5 cut(s)	1368, 1450, 1603, 1642, 1839
VneI	GTGCAC	1 cut(s)	1407
VpaK11BI	GGWCC	4 cut(s)	746, 827, 1586, 1802
XapI	RAATTY	3 cut(s)	602, 1191, 1816
XceI	RCATGY	5 cut(s)	218, 698, 725, 938, 1575
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1528
XhoI	CTCGAG	1 cut(s)	502
XmaJI	CCTAGG	1 cut(s)	634
ZraI	GACGTC	1 cut(s)	1130
Zsp2I	ATGCAT	1 cut(s)	1735

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.