FvH4_4g18400

Protein pleiotropic regulatory locus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002996
Physical Location & Seq
Forward (+)
22286901 .. 22292448
5548 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g18400.t1

Sequence Viewer

Length: 1446 bp
ATGCCTGGTCCGACCTTAGAGATGGAGCGAGTAGAGCCACAGTCGCTGAAAAAGCTCAGTTCCAAATCCCTCAAGCGAGCTCTCGATCTCTTCCCTCCCCTCCATTCCCAATTACCTCCTCCCGATCCTGAGAGTAAAAAAATTCGCATGAGCCACAAGATAAGCGTTGAGTATGGGGGAATTGTACCCACTGCTAGTCAGAAACCCACTCGCACTGCCGATTCTGGTGCTCCCAGTCGTGGACCAGCGGGGCCTTCGAATGCTCTTGCCCTTCCAGACTCTAGAAATGTAAAGACGGAAGCAGGTCAAAATGCTTTGGTGGTTGGTCCATCTGCACCAAAGGAACCGAATCATATTGGAATTTCGGGTAAAGGCACTATAGTTGTTACTGCTCCAGGATCATATGAAAGGAACCCATCAACATCAGCTATAATGGAAAGAATTTCCAGTAGATGGCCGCGTCCTGAGTGGCATGCTCCCTGGAAGATTTACAGGGTTATCAGCGGCCACTTGGGATGGGTACGCTCTATTGCTTTTGATCCAAGTAATAAGTGGTTTTGTACAGGCTCTGCTGATCGAACAATAAAGATATGGGATGTAGGAACTGGACAACTACAGCTTACACTGACAGGACATATTGAGCAAATACGAGGCCTTGCTGTTAGCAGCAGGCATACCTATATGTTCTCTGCTGGTGATGACAAACAAGTCAAATGCTGGGACCTTGAGCAGAACAAGGTTATCCGTTCATACCATGGTCATCTAAGTGGTGTTTACTGCTTGGCCATTCATCCCACTCTCGATATTTTACTTACTGGGGGACGTGATTCAGTATGCCGGGTCTGGGATATTCGTAGCAAGATGCAAGCTCATGCATTGTCCGGGCATGATAACACAGTTTGCTCAGTTTTTACTCGGCCCACGGATCCACAAGTTGTTACTGGCTCCCACGACTCAACCATTAAGTTCTGGGACCTTCGATATGGAAAAACAATGTCAACTATCACCCACCATAAGAAATCTGTACGTGCAATTGCACAACATCCTAAAGAGCATGCTTTTGCATCAGCATCAGCCGACAACATTAAGAAATTCAGCCTTCCAAGAGGGGAATTTTTACACAACATGCTCTCTCAGCAGAAGACCATAATTAATGCTATGGCTATCAATGAGGAGGGAGTTATGGCCACTGGAGGTGACAACGGGAGCTTGTGGTTTTGGGATTGGAAGAGTGGTCACAATTATCAACAATCACAAACAATCGTACAGCCTGGTTCACTGGATAGTGAAGCTGGTATTTATGCACTGTCCTATGATGTTACTGGTACAAGGCTCGTTAGTTGTGAAGCTGACAAGACGATCAAAATGTGGAAACAAGATGAAAATGCCACTCCAGAAACTCATCCTCTCAACTTCAAGCCCCCTAAAGATATCAGACGGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
WD40

Protein Analysis

482

Amino Acids

53.23

Weight (kDa)

9.36

Isoelectric Point (pI)

47.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR41 PF25178 158 - 305 3.3e-12 WDR41
Beta-prop_THOC3 PF25174 161 - 270 2.8e-18 THOC3 beta-propeller domain
WD40_Gbeta PF25391 161 - 303 1.5e-19 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 162 - 283 4.3e-20 WDR3 second beta-propeller domain
WD40 PF00400 162 - 199 4.2e-11 WD domain, G-beta repeat
WD40_Prp19 PF24814 162 - 297 2.5e-23 Prp19 WD40 domain
WD40_MABP1-WDR62_2nd PF24782 164 - 242 6.8e-07 MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz PF24807 164 - 287 9.4e-16 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 165 - 261 3.3e-07 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 166 - 284 1.6e-26 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 167 - 340 7.8e-41 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 174 - 250 4.6e-11 WDHD1 first WD40 domain
WDR55 PF24796 174 - 331 3.5e-17 WDR55
Beta-prop_WDR90_POC16_2nd PF23393 176 - 246 2.7e-06 WDR90/POC16, second beta-propeller
Beta-prop_SPT8 PF23798 176 - 272 1.4e-06 SPT8 beta propeller
Beta-prop_WDR36-Utp21_1st PF25171 181 - 362 5.8e-16 WDR36/Utp21 first beta-propeller
Beta-prop_SCAP PF24017 187 - 337 1.8e-07 SCAP Beta-propeller
WD40 PF00400 203 - 241 2.4e-06 WD domain, G-beta repeat
EIF3I PF24805 207 - 287 6.1e-07 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 208 - 288 4.2e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 209 - 347 4.8e-12 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 210 - 362 5e-15 WDR36/Utp21 second beta-propeller domain
WD40_RFWD3 PF23419 212 - 293 6.3e-06 E3 ubiquitin-protein ligase RFWD3 WD40 domain
Beta-prop_EIPR1 PF23609 212 - 324 8e-08 EIPR1 beta-propeller
WD40_CDC20-Fz PF24807 215 - 457 8.3e-24 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 217 - 416 3.3e-26 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 244 - 361 2.8e-23 WDR3 first beta-propeller domain
WD40 PF00400 246 - 283 1.2e-08 WD domain, G-beta repeat
Beta-prop_EML PF23409 249 - 466 3.4e-16 Echinoderm microtubule-associated protein first beta-propeller
WD40_MABP1-WDR62_2nd PF24782 254 - 456 9.4e-08 MABP1/WDR62 second WD40 domain
WD40_Prp19 PF24814 256 - 457 2.1e-28 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 258 - 418 1.8e-12 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 258 - 418 7.5e-14 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 270 - 463 1.3e-22 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 290 - 364 9.8e-08 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 291 - 325 2e-06 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 293 - 408 2.7e-10 WDR3 second beta-propeller domain
EIF3I PF24805 293 - 418 4.5e-06 EIF3I
Beta-prop_EML_2 PF23414 304 - 461 2.7e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 308 - 437 6.3e-07 WDR90/POC16, second beta-propeller
WD40_Gbeta PF25391 309 - 457 2.9e-07 G protein beta WD-40 repeat protein
WDR55 PF24796 331 - 415 1.5e-07 WDR55
Beta-prop_WDR5 PF25175 334 - 463 8.5e-10 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 707
Acc36I ACCTGC 1 cut(s) 293
AccB7I CCANNNNNTGG 1 cut(s) 453
AccII CGCG 1 cut(s) 460
AciI CCGC 3 cut(s) 248, 458, 504
AclWI GGATC 5 cut(s) 119, 406, 533, 920, 933
AcoI YGGCCR 4 cut(s) 455, 505, 783, 1185
AcsI RAATTY 5 cut(s) 141, 360, 441, 1091, 1112
AfaI GTAC 6 cut(s) 186, 522, 562, 1026, 1266, 1327
AfiI CCNNNNNNNGG 3 cut(s) 239, 453, 844
AgsI TTSAA 1 cut(s) 1417
AjiI CACGTC 1 cut(s) 824
AjnI CCWGG 4 cut(s) 4, 394, 479, 1270
AluBI AGCT 8 cut(s) 55, 80, 428, 619, 869, 1209, 1292, 1349
AluI AGCT 8 cut(s) 55, 80, 428, 619, 869, 1209, 1292, 1349
Alw21I GWGCWC 2 cut(s) 82, 232
AlwI GGATC 5 cut(s) 119, 406, 533, 920, 933
AlwNI CAGNNNCTG 2 cut(s) 46, 569
AoxI GGCC 7 cut(s) 251, 455, 505, 652, 783, 917, 1185
ApeKI GCWGC 1 cut(s) 666
ApoI RAATTY 5 cut(s) 141, 360, 441, 1091, 1112
AseI ATTAAT 1 cut(s) 1152
AspS9I GGNCC 7 cut(s) 8, 242, 251, 326, 721, 918, 973
AsuC2I CCSGG 2 cut(s) 839, 883
AsuHPI GGTGA 3 cut(s) 707, 997, 1208
AsuII TTCGAA 1 cut(s) 257
AvaII GGWCC 5 cut(s) 8, 242, 326, 721, 973
BalI TGGCCA 2 cut(s) 785, 1187
BamHI GGATCC 1 cut(s) 925
BanII GRGCYC 1 cut(s) 82
BbsI GAAGAC 1 cut(s) 1148
Bbv12I GWGCWC 2 cut(s) 82, 232
BbvI GCAGC 1 cut(s) 678
BccI CCATC 5 cut(s) 16, 337, 424, 447, 510
BcgI CGANNNNNNTGC 2 cut(s) 209, 243
BciT130I CCWGG 4 cut(s) 6, 396, 481, 1272
BcnI CCSGG 2 cut(s) 839, 883
BfaI CTAG 3 cut(s) 195, 282, 1444
BfmI CTRYAG 2 cut(s) 378, 614
BfuAI ACCTGC 1 cut(s) 293
BisI GCNGC 3 cut(s) 458, 505, 667
BlsI GCNGC 3 cut(s) 459, 506, 668
Bme1390I CCNGG 6 cut(s) 6, 396, 481, 839, 883, 1272
Bme18I GGWCC 5 cut(s) 8, 242, 326, 721, 973
BmgBI CACGTC 1 cut(s) 824
BmgT120I GGNCC 7 cut(s) 8, 242, 251, 326, 721, 918, 973
BmiI GGNNCC 7 cut(s) 252, 345, 413, 722, 927, 946, 974
BmrFI CCNGG 6 cut(s) 6, 396, 481, 839, 883, 1272
BmrI ACTGGG 2 cut(s) 228, 825
BmsI GCATC 3 cut(s) 852, 1073, 1079
BmuI ACTGGG 2 cut(s) 228, 825
BpiI GAAGAC 1 cut(s) 1148
BpmI CTGGAG 3 cut(s) 378, 1212, 1377
Bpu14I TTCGAA 1 cut(s) 257
BpuEI CTTGAG 2 cut(s) 56, 746
BpuMI CCSGG 2 cut(s) 839, 883
BsaAI YACGTR 1 cut(s) 1028
BsaJI CCNNGG 3 cut(s) 479, 754, 921
Bsc4I CCNNNNNNNGG 3 cut(s) 239, 453, 844
Bse1I ACTGG 8 cut(s) 234, 447, 610, 820, 946, 1195, 1284, 1327
BseBI CCWGG 4 cut(s) 6, 396, 481, 1272
BseDI CCNNGG 3 cut(s) 479, 754, 921
BseGI GGATG 5 cut(s) 521, 601, 790, 1042, 1402
BseLI CCNNNNNNNGG 3 cut(s) 239, 453, 844
BseMII CTCAG 5 cut(s) 70, 120, 456, 918, 1148
BseNI ACTGG 8 cut(s) 234, 447, 610, 820, 946, 1195, 1284, 1327
BseRI GAGGAG 2 cut(s) 108, 1187
BseXI GCAGC 1 cut(s) 678
BseYI CCCAGC 1 cut(s) 717
BsgI GTGCAG 1 cut(s) 318
Bsh1236I CGCG 1 cut(s) 460
BshFI GGCC 7 cut(s) 253, 457, 507, 654, 785, 919, 1187
BsiHKAI GWGCWC 2 cut(s) 82, 232
BsiSI CCGG 2 cut(s) 838, 882
BslFI GGGAC 3 cut(s) 734, 834, 986
BslI CCNNNNNNNGG 3 cut(s) 239, 453, 844
BsmFI GGGAC 3 cut(s) 734, 834, 986
BsmI GAATGC 1 cut(s) 265
BsnI GGCC 7 cut(s) 253, 457, 507, 654, 785, 919, 1187
Bsp119I TTCGAA 1 cut(s) 257
Bsp1286I GDGCHC 2 cut(s) 82, 232
Bsp1407I TGTACA 1 cut(s) 560
Bsp143I GATC 7 cut(s) 85, 124, 398, 538, 574, 925, 1359
Bsp19I CCATGG 1 cut(s) 754
BspACI CCGC 3 cut(s) 248, 458, 504
BspANI GGCC 7 cut(s) 253, 457, 507, 654, 785, 919, 1187
BspCNI CTCAG 5 cut(s) 69, 121, 457, 917, 1147
BspFNI CGCG 1 cut(s) 460
BspLI GGNNCC 7 cut(s) 252, 345, 413, 722, 927, 946, 974
BspMI ACCTGC 1 cut(s) 293
BspPI GGATC 5 cut(s) 119, 406, 533, 920, 933
BspT104I TTCGAA 1 cut(s) 257
BsrGI TGTACA 1 cut(s) 560
BsrI ACTGG 8 cut(s) 234, 447, 610, 820, 946, 1195, 1284, 1327
BssECI CCNNGG 3 cut(s) 479, 754, 921
BssMI GATC 7 cut(s) 85, 124, 398, 538, 574, 925, 1359
BssT1I CCWWGG 1 cut(s) 754
Bst2UI CCWGG 4 cut(s) 6, 396, 481, 1272
Bst4CI ACNGT 4 cut(s) 42, 898, 1308, 1440
Bst6I CTCTTC 2 cut(s) 95, 1223
BstAUI TGTACA 1 cut(s) 560
BstBAI YACGTR 1 cut(s) 1028
BstBI TTCGAA 1 cut(s) 257
BstC8I GCNNGC 5 cut(s) 78, 474, 671, 867, 1056
BstDEI CTNAG 7 cut(s) 16, 56, 129, 465, 764, 904, 1134
BstDSI CCRYGG 2 cut(s) 754, 921
BstF5I GGATG 5 cut(s) 521, 601, 790, 1042, 1402
BstFNI CGCG 1 cut(s) 460
BstKTI GATC 7 cut(s) 88, 127, 401, 541, 577, 928, 1362
BstMBI GATC 7 cut(s) 85, 124, 398, 538, 574, 925, 1359
BstMWI GCNNNNNNNGC 4 cut(s) 34, 43, 52, 1135
BstNI CCWGG 4 cut(s) 6, 396, 481, 1272
BstNSI RCATGY 3 cut(s) 476, 1058, 1129
BstSCI CCNGG 6 cut(s) 4, 394, 479, 837, 881, 1270
BstSFI CTRYAG 2 cut(s) 378, 614
BstUI CGCG 1 cut(s) 460
BstV1I GCAGC 1 cut(s) 678
BstV2I GAAGAC 1 cut(s) 1148
BstX2I RGATCY 1 cut(s) 925
BstYI RGATCY 1 cut(s) 925
BsuRI GGCC 7 cut(s) 253, 457, 507, 654, 785, 919, 1187
BtgI CCRYGG 2 cut(s) 754, 921
BtrI CACGTC 1 cut(s) 824
BtsCI GGATG 5 cut(s) 521, 601, 790, 1042, 1402
BtsI GCAGTG 2 cut(s) 189, 213
BtsIMutI CAGTG 6 cut(s) 189, 213, 623, 1188, 1277, 1304
BveI ACCTGC 1 cut(s) 293
Cac8I GCNNGC 5 cut(s) 78, 474, 671, 867, 1056
CaiI CAGNNNCTG 2 cut(s) 46, 569
Cfr13I GGNCC 7 cut(s) 8, 242, 251, 326, 721, 918, 973
CseI GACGC 1 cut(s) 449
Csp6I GTAC 6 cut(s) 185, 521, 561, 1025, 1265, 1326
CviAII CATG 7 cut(s) 148, 473, 755, 872, 887, 1055, 1126
CviQI GTAC 6 cut(s) 185, 521, 561, 1025, 1265, 1326
DdeI CTNAG 7 cut(s) 16, 56, 129, 465, 764, 904, 1134
DpnI GATC 7 cut(s) 87, 126, 400, 540, 576, 927, 1361
DpnII GATC 7 cut(s) 85, 124, 398, 538, 574, 925, 1359
DrdI GACNNNNNNGTC 1 cut(s) 707
DseDI GACNNNNNNGTC 1 cut(s) 707
EaeI YGGCCR 4 cut(s) 455, 505, 783, 1185
Eam1104I CTCTTC 2 cut(s) 95, 1223
EarI CTCTTC 2 cut(s) 95, 1223
Ecl136II GAGCTC 1 cut(s) 80
Eco130I CCWWGG 1 cut(s) 754
Eco147I AGGCCT 1 cut(s) 654
Eco24I GRGCYC 1 cut(s) 82
Eco32I GATATC 1 cut(s) 1432
Eco47I GGWCC 5 cut(s) 8, 242, 326, 721, 973
Eco53kI GAGCTC 1 cut(s) 80
EcoICRI GAGCTC 1 cut(s) 80
EcoO109I RGGNCCY 3 cut(s) 251, 721, 973
EcoRII CCWGG 4 cut(s) 4, 394, 479, 1270
EcoRV GATATC 1 cut(s) 1432
EcoT14I CCWWGG 1 cut(s) 754
EcoT22I ATGCAT 1 cut(s) 877
EcoT38I GRGCYC 1 cut(s) 82
ErhI CCWWGG 1 cut(s) 754
FaeI CATG 7 cut(s) 151, 476, 758, 875, 890, 1058, 1129
FaqI GGGAC 3 cut(s) 734, 834, 986
FatI CATG 7 cut(s) 147, 472, 754, 871, 886, 1054, 1125
FauI CCCGC 1 cut(s) 241
FauNDI CATATG 1 cut(s) 403
Fnu4HI GCNGC 3 cut(s) 458, 505, 667
FokI GGATG 5 cut(s) 528, 608, 777, 1029, 1389
FriOI GRGCYC 1 cut(s) 82
Fsp4HI GCNGC 3 cut(s) 458, 505, 667
FspBI CTAG 3 cut(s) 195, 282, 1444
GluI GCNGC 3 cut(s) 458, 505, 667
GsaI CCCAGC 1 cut(s) 721
GsuI CTGGAG 3 cut(s) 378, 1212, 1377
HaeIII GGCC 7 cut(s) 253, 457, 507, 654, 785, 919, 1187
HapII CCGG 2 cut(s) 838, 882
HgaI GACGC 1 cut(s) 449
Hin1II CATG 7 cut(s) 151, 476, 758, 875, 890, 1058, 1129
HincII GTYRAC 1 cut(s) 999
HindII GTYRAC 1 cut(s) 999
HinfI GANTC 5 cut(s) 221, 278, 349, 827, 953
HpaII CCGG 2 cut(s) 838, 882
HphI GGTGA 3 cut(s) 707, 997, 1208
Hpy166II GTNNAC 4 cut(s) 242, 775, 999, 1277
Hpy188I TCNGA 3 cut(s) 12, 201, 1436
Hpy188III TCNNGA 8 cut(s) 83, 122, 128, 275, 282, 464, 800, 1394
Hpy8I GTNNAC 4 cut(s) 242, 775, 999, 1277
HpyAV CCTTC 4 cut(s) 264, 281, 986, 1109
HpyCH4III ACNGT 4 cut(s) 42, 898, 1308, 1440
HpyCH4IV ACGT 2 cut(s) 823, 1027
HpyCH4V TGCA 7 cut(s) 335, 865, 875, 1031, 1037, 1064, 1304
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 43, 52, 1135
HpyF3I CTNAG 7 cut(s) 16, 56, 129, 465, 764, 904, 1134
HpySE526I ACGT 2 cut(s) 823, 1027
Hsp92II CATG 7 cut(s) 151, 476, 758, 875, 890, 1058, 1129
Kzo9I GATC 7 cut(s) 85, 124, 398, 538, 574, 925, 1359
LmnI GCTCC 6 cut(s) 25, 235, 397, 481, 950, 1206
Lsp1109I GCAGC 1 cut(s) 678
LweI GCATC 3 cut(s) 852, 1073, 1079
MaeI CTAG 3 cut(s) 195, 282, 1444
MaeII ACGT 2 cut(s) 823, 1027
MaeIII GTNAC 5 cut(s) 385, 937, 1196, 1235, 1318
MalI GATC 7 cut(s) 87, 126, 400, 540, 576, 927, 1361
MboI GATC 7 cut(s) 85, 124, 398, 538, 574, 925, 1359
MboII GAAGA 4 cut(s) 82, 496, 1153, 1240
MfeI CAATTG 1 cut(s) 1032
MflI RGATCY 1 cut(s) 925
MhlI GDGCHC 2 cut(s) 82, 232
MlsI TGGCCA 2 cut(s) 785, 1187
MluNI TGGCCA 2 cut(s) 785, 1187
MlyI GAGTC 2 cut(s) 272, 947
MmeI TCCRAC 1 cut(s) 35
Mox20I TGGCCA 2 cut(s) 785, 1187
Mph1103I ATGCAT 1 cut(s) 877
MscI TGGCCA 2 cut(s) 785, 1187
MseI TTAA 3 cut(s) 963, 1086, 1152
MslI CAYNNNNRTG 1 cut(s) 765
Msp20I TGGCCA 2 cut(s) 785, 1187
MspA1I CMGCKG 2 cut(s) 248, 504
MspI CCGG 2 cut(s) 838, 882
MspR9I CCNGG 6 cut(s) 6, 396, 481, 839, 883, 1272
MunI CAATTG 1 cut(s) 1032
Mva1269I GAATGC 1 cut(s) 265
MvaI CCWGG 4 cut(s) 6, 396, 481, 1272
MvnI CGCG 1 cut(s) 460
MwoI GCNNNNNNNGC 4 cut(s) 34, 43, 52, 1135
NciI CCSGG 2 cut(s) 839, 883
NcoI CCATGG 1 cut(s) 754
NdeI CATATG 1 cut(s) 403
NdeII GATC 7 cut(s) 85, 124, 398, 538, 574, 925, 1359
NlaIII CATG 7 cut(s) 151, 476, 758, 875, 890, 1058, 1129
NlaIV GGNNCC 7 cut(s) 252, 345, 413, 722, 927, 946, 974
NmeAIII GCCGAG 1 cut(s) 895
NmuCI GTSAC 2 cut(s) 1196, 1235
NsiI ATGCAT 1 cut(s) 877
NspI RCATGY 3 cut(s) 476, 1058, 1129
NspV TTCGAA 1 cut(s) 257
PaeI GCATGC 2 cut(s) 476, 1058
PceI AGGCCT 1 cut(s) 654
PctI GAATGC 1 cut(s) 265
PfeI GAWTC 3 cut(s) 221, 349, 827
PflMI CCANNNNNTGG 1 cut(s) 453
PfoI TCCNGGA 1 cut(s) 394
PkrI GCNGC 3 cut(s) 459, 506, 668
PleI GAGTC 2 cut(s) 272, 947
PpsI GAGTC 2 cut(s) 272, 947
Ppu21I YACGTR 1 cut(s) 1028
PpuMI RGGWCCY 2 cut(s) 721, 973
PshBI ATTAAT 1 cut(s) 1152
Psp124BI GAGCTC 1 cut(s) 82
Psp5II RGGWCCY 2 cut(s) 721, 973
Psp6I CCWGG 4 cut(s) 4, 394, 479, 1270
PspFI CCCAGC 1 cut(s) 717
PspGI CCWGG 4 cut(s) 4, 394, 479, 1270
PspN4I GGNNCC 7 cut(s) 252, 345, 413, 722, 927, 946, 974
PspPI GGNCC 7 cut(s) 8, 242, 251, 326, 721, 918, 973
PspPPI RGGWCCY 2 cut(s) 721, 973
PstNI CAGNNNCTG 2 cut(s) 46, 569
PsuI RGATCY 1 cut(s) 925
RsaI GTAC 6 cut(s) 186, 522, 562, 1026, 1266, 1327
RsaNI GTAC 6 cut(s) 185, 521, 561, 1025, 1265, 1326
RseI CAYNNNNRTG 1 cut(s) 765
SacI GAGCTC 1 cut(s) 82
SaqAI TTAA 3 cut(s) 963, 1086, 1152
SatI GCNGC 3 cut(s) 458, 505, 667
Sau3AI GATC 7 cut(s) 85, 124, 398, 538, 574, 925, 1359
Sau96I GGNCC 7 cut(s) 8, 242, 251, 326, 721, 918, 973
SchI GAGTC 2 cut(s) 272, 947
ScrFI CCNGG 6 cut(s) 6, 396, 481, 839, 883, 1272
SduI GDGCHC 2 cut(s) 82, 232
SfaNI GCATC 3 cut(s) 852, 1073, 1079
SfcI CTRYAG 2 cut(s) 378, 614
SfuI TTCGAA 1 cut(s) 257
SinI GGWCC 5 cut(s) 8, 242, 326, 721, 973
SmiMI CAYNNNNRTG 1 cut(s) 765
SmlI CTYRAG 2 cut(s) 71, 725
SmoI CTYRAG 2 cut(s) 71, 725
SphI GCATGC 2 cut(s) 476, 1058
SseBI AGGCCT 1 cut(s) 654
SsiI CCGC 3 cut(s) 248, 458, 504
SspMI CTAG 3 cut(s) 195, 282, 1444
SstI GAGCTC 1 cut(s) 82
StuI AGGCCT 1 cut(s) 654
StyD4I CCNGG 6 cut(s) 4, 394, 479, 837, 881, 1270
StyI CCWWGG 1 cut(s) 754
TaaI ACNGT 4 cut(s) 42, 898, 1308, 1440
TaiI ACGT 2 cut(s) 826, 1030
TaqI TCGA 5 cut(s) 84, 257, 577, 801, 979
TatI WGTACW 1 cut(s) 560
TauI GCSGC 2 cut(s) 460, 507
TfiI GAWTC 3 cut(s) 221, 349, 827
Tru1I TTAA 3 cut(s) 963, 1086, 1152
Tru9I TTAA 3 cut(s) 963, 1086, 1152
TscAI CASTG 6 cut(s) 196, 220, 630, 1195, 1284, 1311
TseFI GTSAC 2 cut(s) 1196, 1235
TseI GCWGC 1 cut(s) 666
Tsp45I GTSAC 2 cut(s) 1196, 1235
TspDTI ATGAA 4 cut(s) 420, 738, 779, 1395
TspGWI ACGGA 3 cut(s) 311, 734, 938
TspRI CASTG 6 cut(s) 196, 220, 630, 1195, 1284, 1311
Van91I CCANNNNNTGG 1 cut(s) 453
VpaK11BI GGWCC 5 cut(s) 8, 242, 326, 721, 973
VspI ATTAAT 1 cut(s) 1152
XapI RAATTY 5 cut(s) 141, 360, 441, 1091, 1112
XbaI TCTAGA 1 cut(s) 281
XceI RCATGY 3 cut(s) 476, 1058, 1129
XcmI CCANNNNNNNNNTGG 1 cut(s) 549
XspI CTAG 3 cut(s) 195, 282, 1444
Zsp2I ATGCAT 1 cut(s) 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.