MD00G1128100.v1.1

Protein pleiotropic regulatory locus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0002996
Physical Location & Seq
Reverse (-)
27202204 .. 27209184
6981 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1128100.v1.1.491

Sequence Viewer

Length: 1404 bp
ATGCCCGGGCCCACCTTGGAGATGGAACCAGTGGAGCCACAGTCGCTGAAGAAGCTCAGTCTCAAATCCCTCAAACGATCCCTCGATCTCTTCTCCCCCGCCCATGGCGAACTCCCCCCTCCCGACCCCGAGAGTAAAAGAATTCGCATGAGTCACAAGATAAGCATGGAATATGGAGGAATTCAACCCACACCTAGCCAAAAACCACCTCGCCGGGCTGAGTCTGTTGCACCGATTCTAAGGATGCGCAGACGAGAGGAGCTCAAAATTCTTTGGTTGTCGGTCCTTCTGCACCAAGGGCACTGTGAGAATACTGTTGGTATTTCGGGTAAAGGCACTTTAGCTGTTTCTGCTCCAGGGTCATCTGAAAGGTTTTCAACATCGGCCATGATGGAAAGGATTTCCAGCAGATGGCCACGTCCTGATTGGCATGCTCCTTGGAAAATCTACAGAGTCATTAGCGGCCACTTGGGATGGGTACGAGCTATTGCCTTTGATCCAAGTAATAAATGGTTCTGCACTGGCTCAGCAGACCGTACTATCAAGATTTGGGATGTTGCAACTGGAACTCTACAACTGACAGTGACAGGACACATCGAGCAAATAAGAGGCCTTGCGGTTAGCAGCAGGCATACCTATATGTTCTCTGCTGGAGATGACAAACTAGTTAAATGCTGGGATCTTGAACAGAATAAGGTTATCCGTTCATATCATGGTCATCTAAGTGGTGTTTACTGCTTGGCTCTTCATCCCACTCTTGATGTTTTACTTACCGGGGGACGTGATTCTGTGTGCCGGGTTTGGGATATTCGAAGCAAGATGCAAGTTCATGCGTTGTCAGGGCATGACAACACAGTTTGTTCCGTTTTTACTCGACCAACAGATCCACAAGTTGTTACTGGCTCTCACGACACAACCATCAAGTTCTGGGACCTTAGATATGGAAAAACAATGTCAACTCTGACGCACCATAAAAAGTCTGTGCGTGCAATGGCCCAGCATCCTAAAGAGCAATGTTTTGCATCTGCATCAGCTGATAATATTAAGAAGTTCAGCCTCCCAAGAGGCGAATTTTTACACAACATGAGCTCTCAGCAGAAAACAATAATCAATGCAATGGCTGTCAACGAGGATGGAGTTATGGCCACTGGAGGCGATAATGGGAGCATGTGGTTCTGGGATTGGAAGAGCGGTCACAATTTTCAGCAATCCCAAACAATTGTACAGCCTGGCTCGCTGGACAGTGAAGCTGGTATATATGCTCTTTCCTACGACATCACGGGTACGAGGCTGGTCAGTTGTGAAGCCGATAAGACTATCAAGATGTGGAAGCAAGATGAAAATGCCACTCCAGAAACCCACCCTCTCAACTTTAGGCCCCCGAAAGACATTCGACGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
WD40

Protein Analysis

468

Amino Acids

52.51

Weight (kDa)

9.33

Isoelectric Point (pI)

51.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Gbeta PF25391 133 - 289 5.4e-19 G protein beta WD-40 repeat protein
Beta-prop_WDR41 PF25178 144 - 293 2.3e-11 WDR41
Beta-prop_THOC3 PF25174 147 - 213 1.5e-11 THOC3 beta-propeller domain
WD40_Prp19 PF24814 148 - 283 1.3e-22 Prp19 WD40 domain
WD40 PF00400 148 - 185 6.5e-11 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 148 - 246 2.8e-07 TEP-1 second beta-propeller
Beta-prop_WDR3_2nd PF25172 149 - 269 8e-19 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 150 - 272 9.8e-16 CDC20/Fizzy WD40 domain
WD40_MABP1-WDR62_2nd PF24782 150 - 228 3.3e-07 MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_1st PF25173 152 - 268 2.7e-25 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 153 - 325 9.5e-41 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 160 - 236 8e-11 WDHD1 first WD40 domain
WDR55 PF24796 161 - 317 6.8e-17 WDR55
Beta-prop_WDR90_POC16_2nd PF23393 162 - 232 1.4e-06 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 167 - 348 3e-16 WDR36/Utp21 first beta-propeller
EIF3I PF24805 194 - 278 4.1e-06 EIF3I
Beta-prop_WDR36-Utp21_2nd PF25168 196 - 348 8.7e-16 WDR36/Utp21 second beta-propeller domain
WD40_RFWD3 PF23419 198 - 289 2.4e-06 E3 ubiquitin-protein ligase RFWD3 WD40 domain
Beta-prop_EIPR1 PF23609 198 - 310 5.7e-07 EIPR1 beta-propeller
Beta-prop_THOC3 PF25174 201 - 402 7e-28 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 203 - 443 5.1e-23 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 230 - 347 2.2e-24 WDR3 first beta-propeller domain
WD40 PF00400 232 - 269 2e-08 WD domain, G-beta repeat
Beta-prop_EML PF23409 235 - 452 4.1e-16 Echinoderm microtubule-associated protein first beta-propeller
WD40_MABP1-WDR62_2nd PF24782 239 - 442 1.1e-07 MABP1/WDR62 second WD40 domain
WD40_Prp19 PF24814 242 - 443 1e-29 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 244 - 404 2.8e-15 WDHD1 first WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 253 - 344 4.5e-08 WDR90/POC16, second beta-propeller
Beta-prop_TEP1_2nd PF25047 254 - 335 1.8e-08 TEP-1 second beta-propeller
Beta-prop_SCAP PF24017 255 - 402 4.8e-07 SCAP Beta-propeller
WD40 PF00400 275 - 311 1.5e-06 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 276 - 352 1.8e-08 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 278 - 405 3.5e-06 EIF3I
Beta-prop_EML_2 PF23414 290 - 446 3.3e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 294 - 397 1.9e-07 WDR90/POC16, second beta-propeller
WD40_Gbeta PF25391 295 - 443 7.7e-08 G protein beta WD-40 repeat protein
WDR55 PF24796 317 - 402 7.3e-08 WDR55
Beta-prop_WDR5 PF25175 320 - 449 3.1e-10 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 328 - 449 3.9e-06 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 341 - 444 5.4e-06 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 248
AccB7I CCANNNNNTGG 1 cut(s) 411
AccBSI CCGCTC 1 cut(s) 1191
AciI CCGC 4 cut(s) 99, 462, 617, 1191
AclWI GGATC 4 cut(s) 72, 491, 687, 878
AcoI YGGCCR 4 cut(s) 384, 413, 463, 1143
AcsI RAATTY 4 cut(s) 141, 180, 267, 1070
AcuI CTGAAG 1 cut(s) 68
AfaI GTAC 4 cut(s) 480, 538, 1224, 1285
AfiI CCNNNNNNNGG 3 cut(s) 104, 411, 802
AgsI TTSAA 3 cut(s) 185, 378, 686
AhlI ACTAGT 1 cut(s) 664
AjiI CACGTC 2 cut(s) 419, 782
AjnI CCWGG 2 cut(s) 355, 1228
AluBI AGCT 7 cut(s) 55, 262, 344, 485, 1034, 1089, 1250
AluI AGCT 7 cut(s) 55, 262, 344, 485, 1034, 1089, 1250
Alw21I GWGCWC 2 cut(s) 264, 1091
Alw26I GTCTC 1 cut(s) 65
AlwI GGATC 4 cut(s) 72, 491, 687, 878
AlwNI CAGNNNCTG 1 cut(s) 46
Ama87I CYCGRG 2 cut(s) 5, 128
AoxI GGCC 8 cut(s) 8, 384, 413, 463, 610, 993, 1143, 1376
ApaI GGGCCC 1 cut(s) 12
ApeKI GCWGC 1 cut(s) 624
ApoI RAATTY 4 cut(s) 141, 180, 267, 1070
AspLEI GCGC 1 cut(s) 249
AspS9I GGNCC 6 cut(s) 8, 9, 283, 931, 994, 1377
AsuC2I CCSGG 5 cut(s) 6, 7, 215, 775, 797
AsuII TTCGAA 1 cut(s) 811
AvaI CYCGRG 2 cut(s) 5, 128
AvaII GGWCC 2 cut(s) 283, 931
BaeGI GKGCMC 2 cut(s) 12, 303
BalI TGGCCA 2 cut(s) 415, 1145
BanII GRGCYC 3 cut(s) 12, 264, 1091
Bbv12I GWGCWC 2 cut(s) 264, 1091
BbvI GCAGC 1 cut(s) 636
BccI CCATC 6 cut(s) 16, 385, 405, 468, 926, 1127
BciT130I CCWGG 2 cut(s) 357, 1230
BcnI CCSGG 5 cut(s) 6, 7, 215, 775, 797
BcoDI GTCTC 1 cut(s) 65
BcuI ACTAGT 1 cut(s) 664
BfaI CTAG 2 cut(s) 195, 665
BfmI CTRYAG 1 cut(s) 448
BisI GCNGC 2 cut(s) 463, 625
BlpI GCTNAGC 1 cut(s) 526
BlsI GCNGC 2 cut(s) 464, 626
Bme1390I CCNGG 7 cut(s) 6, 7, 215, 357, 775, 797, 1230
Bme18I GGWCC 2 cut(s) 283, 931
BmeT110I CYCGRG 2 cut(s) 5, 128
BmgBI CACGTC 2 cut(s) 419, 782
BmgT120I GGNCC 6 cut(s) 8, 9, 283, 931, 994, 1377
BmiI GGNNCC 5 cut(s) 10, 27, 36, 932, 1379
BmrFI CCNGG 7 cut(s) 6, 7, 215, 357, 775, 797, 1230
BmsI GCATC 5 cut(s) 234, 810, 1009, 1031, 1037
BplI GAGNNNNNCTC 2 cut(s) 246, 278
BpmI CTGGAG 4 cut(s) 339, 672, 1170, 1335
Bpu1102I GCTNAGC 1 cut(s) 526
Bpu14I TTCGAA 1 cut(s) 811
BpuMI CCSGG 5 cut(s) 6, 7, 215, 775, 797
BsaJI CCNNGG 7 cut(s) 5, 15, 103, 295, 356, 437, 774
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bsc4I CCNNNNNNNGG 3 cut(s) 104, 411, 802
Bse1I ACTGG 5 cut(s) 29, 526, 568, 904, 1153
Bse3DI GCAATG 3 cut(s) 996, 1019, 1122
BseBI CCWGG 2 cut(s) 357, 1230
BseDI CCNNGG 7 cut(s) 5, 15, 103, 295, 356, 437, 774
BseGI GGATG 6 cut(s) 249, 479, 559, 748, 1000, 1138
BseLI CCNNNNNNNGG 3 cut(s) 104, 411, 802
BseMI GCAATG 3 cut(s) 996, 1019, 1122
BseMII CTCAG 4 cut(s) 70, 210, 540, 1106
BseNI ACTGG 5 cut(s) 29, 526, 568, 904, 1153
BseRI GAGGAG 1 cut(s) 272
BseSI GKGCMC 2 cut(s) 12, 303
BseXI GCAGC 1 cut(s) 636
BseYI CCCAGC 2 cut(s) 675, 996
BsgI GTGCAG 2 cut(s) 275, 502
BshFI GGCC 8 cut(s) 10, 386, 415, 465, 612, 995, 1145, 1378
BsiHKAI GWGCWC 2 cut(s) 264, 1091
BsiHKCI CYCGRG 2 cut(s) 5, 128
BsiSI CCGG 4 cut(s) 6, 214, 774, 796
BslFI GGGAC 2 cut(s) 792, 944
BslI CCNNNNNNNGG 3 cut(s) 104, 411, 802
BsmAI GTCTC 1 cut(s) 65
BsmFI GGGAC 2 cut(s) 792, 944
BsnI GGCC 8 cut(s) 10, 386, 415, 465, 612, 995, 1145, 1378
BsoBI CYCGRG 2 cut(s) 5, 128
Bsp119I TTCGAA 1 cut(s) 811
Bsp120I GGGCCC 1 cut(s) 8
Bsp1286I GDGCHC 4 cut(s) 12, 264, 303, 1091
Bsp1407I TGTACA 1 cut(s) 1222
Bsp143I GATC 5 cut(s) 77, 85, 496, 679, 883
Bsp1720I GCTNAGC 1 cut(s) 526
Bsp19I CCATGG 1 cut(s) 103
BspACI CCGC 4 cut(s) 99, 462, 617, 1191
BspANI GGCC 8 cut(s) 10, 386, 415, 465, 612, 995, 1145, 1378
BspCNI CTCAG 4 cut(s) 69, 211, 539, 1105
BspLI GGNNCC 5 cut(s) 10, 27, 36, 932, 1379
BspPI GGATC 4 cut(s) 72, 491, 687, 878
BspQI GCTCTTC 2 cut(s) 750, 1181
BspT104I TTCGAA 1 cut(s) 811
BsrBI CCGCTC 1 cut(s) 1191
BsrDI GCAATG 3 cut(s) 996, 1019, 1122
BsrGI TGTACA 1 cut(s) 1222
BsrI ACTGG 5 cut(s) 29, 526, 568, 904, 1153
BssECI CCNNGG 7 cut(s) 5, 15, 103, 295, 356, 437, 774
BssMI GATC 5 cut(s) 77, 85, 496, 679, 883
BssT1I CCWWGG 4 cut(s) 15, 103, 295, 437
Bst2UI CCWGG 2 cut(s) 357, 1230
Bst4CI ACNGT 8 cut(s) 42, 305, 316, 536, 583, 856, 1244, 1398
Bst6I CTCTTC 3 cut(s) 95, 750, 1181
BstAUI TGTACA 1 cut(s) 1222
BstBI TTCGAA 1 cut(s) 811
BstC8I GCNNGC 4 cut(s) 432, 629, 987, 1235
BstDEI CTNAG 7 cut(s) 56, 219, 239, 526, 722, 935, 1092
BstDSI CCRYGG 1 cut(s) 103
BstF5I GGATG 6 cut(s) 249, 479, 559, 748, 1000, 1138
BstHHI GCGC 1 cut(s) 249
BstKTI GATC 5 cut(s) 80, 88, 499, 682, 886
BstMAI GTCTC 1 cut(s) 65
BstMBI GATC 5 cut(s) 77, 85, 496, 679, 883
BstMWI GCNNNNNNNGC 5 cut(s) 43, 52, 298, 350, 1234
BstNI CCWGG 2 cut(s) 357, 1230
BstNSI RCATGY 2 cut(s) 434, 1171
BstSCI CCNGG 7 cut(s) 4, 5, 213, 355, 773, 795, 1228
BstSFI CTRYAG 1 cut(s) 448
BstSLI GKGCMC 2 cut(s) 12, 303
BstV1I GCAGC 1 cut(s) 636
BstX2I RGATCY 2 cut(s) 679, 883
BstYI RGATCY 2 cut(s) 679, 883
BsuRI GGCC 8 cut(s) 10, 386, 415, 465, 612, 995, 1145, 1378
BtgI CCRYGG 1 cut(s) 103
BtrI CACGTC 2 cut(s) 419, 782
BtsCI GGATG 6 cut(s) 249, 479, 559, 748, 1000, 1138
BtsIMutI CAGTG 6 cut(s) 36, 301, 519, 588, 1146, 1249
Cac8I GCNNGC 4 cut(s) 432, 629, 987, 1235
CaiI CAGNNNCTG 1 cut(s) 46
CfoI GCGC 1 cut(s) 249
Cfr13I GGNCC 6 cut(s) 8, 9, 283, 931, 994, 1377
Cfr9I CCCGGG 1 cut(s) 5
CseI GACGC 1 cut(s) 973
Csp6I GTAC 4 cut(s) 479, 537, 1223, 1284
CviQI GTAC 4 cut(s) 479, 537, 1223, 1284
DdeI CTNAG 7 cut(s) 56, 219, 239, 526, 722, 935, 1092
DpnI GATC 5 cut(s) 79, 87, 498, 681, 885
DpnII GATC 5 cut(s) 77, 85, 496, 679, 883
EaeI YGGCCR 4 cut(s) 384, 413, 463, 1143
Eam1104I CTCTTC 3 cut(s) 95, 750, 1181
EarI CTCTTC 3 cut(s) 95, 750, 1181
Ecl136II GAGCTC 2 cut(s) 262, 1089
Eco130I CCWWGG 4 cut(s) 15, 103, 295, 437
Eco147I AGGCCT 1 cut(s) 612
Eco24I GRGCYC 3 cut(s) 12, 264, 1091
Eco47I GGWCC 2 cut(s) 283, 931
Eco53kI GAGCTC 2 cut(s) 262, 1089
Eco57I CTGAAG 1 cut(s) 68
Eco88I CYCGRG 2 cut(s) 5, 128
EcoICRI GAGCTC 2 cut(s) 262, 1089
EcoO109I RGGNCCY 2 cut(s) 931, 1377
EcoRI GAATTC 2 cut(s) 141, 180
EcoRII CCWGG 2 cut(s) 355, 1228
EcoT14I CCWWGG 4 cut(s) 15, 103, 295, 437
EcoT38I GRGCYC 3 cut(s) 12, 264, 1091
ErhI CCWWGG 4 cut(s) 15, 103, 295, 437
FaqI GGGAC 2 cut(s) 792, 944
FauI CCCGC 1 cut(s) 106
Fnu4HI GCNGC 2 cut(s) 463, 625
FokI GGATG 6 cut(s) 256, 486, 566, 735, 987, 1145
FriOI GRGCYC 3 cut(s) 12, 264, 1091
Fsp4HI GCNGC 2 cut(s) 463, 625
FspBI CTAG 2 cut(s) 195, 665
FspI TGCGCA 1 cut(s) 248
GlaI GCGC 1 cut(s) 248
GluI GCNGC 2 cut(s) 463, 625
GsaI CCCAGC 2 cut(s) 679, 1000
GsuI CTGGAG 4 cut(s) 339, 672, 1170, 1335
HaeIII GGCC 8 cut(s) 10, 386, 415, 465, 612, 995, 1145, 1378
HapII CCGG 4 cut(s) 6, 214, 774, 796
HgaI GACGC 1 cut(s) 973
HhaI GCGC 1 cut(s) 249
Hin6I GCGC 1 cut(s) 247
HinP1I GCGC 1 cut(s) 247
HincII GTYRAC 2 cut(s) 957, 1126
HindII GTYRAC 2 cut(s) 957, 1126
HinfI GANTC 5 cut(s) 151, 221, 235, 453, 785
HpaII CCGG 4 cut(s) 6, 214, 774, 796
Hpy166II GTNNAC 3 cut(s) 733, 957, 1126
Hpy188I TCNGA 2 cut(s) 367, 963
Hpy188III TCNNGA 8 cut(s) 122, 422, 544, 683, 758, 908, 1321, 1352
Hpy8I GTNNAC 3 cut(s) 733, 957, 1126
Hpy99I CGWCG 1 cut(s) 1398
HpyAV CCTTC 1 cut(s) 296
HpyCH4III ACNGT 8 cut(s) 42, 305, 316, 536, 583, 856, 1244, 1398
HpyCH4IV ACGT 2 cut(s) 418, 781
HpyCH4V TGCA 9 cut(s) 230, 292, 519, 560, 823, 989, 1022, 1028, 1115
HpyF10VI GCNNNNNNNGC 5 cut(s) 43, 52, 298, 350, 1234
HpyF3I CTNAG 7 cut(s) 56, 219, 239, 526, 722, 935, 1092
HpySE526I ACGT 2 cut(s) 418, 781
HspAI GCGC 1 cut(s) 247
Kzo9I GATC 5 cut(s) 77, 85, 496, 679, 883
LguI GCTCTTC 2 cut(s) 750, 1181
LmnI GCTCC 5 cut(s) 34, 259, 358, 439, 1164
Lsp1109I GCAGC 1 cut(s) 636
LweI GCATC 5 cut(s) 234, 810, 1009, 1031, 1037
MaeI CTAG 2 cut(s) 195, 665
MaeII ACGT 2 cut(s) 418, 781
MaeIII GTNAC 4 cut(s) 152, 583, 895, 1193
MalI GATC 5 cut(s) 79, 87, 498, 681, 885
MbiI CCGCTC 1 cut(s) 1191
MboI GATC 5 cut(s) 77, 85, 496, 679, 883
MboII GAAGA 4 cut(s) 61, 82, 737, 1198
MfeI CAATTG 1 cut(s) 1218
MflI RGATCY 2 cut(s) 679, 883
MhlI GDGCHC 4 cut(s) 12, 264, 303, 1091
MlsI TGGCCA 2 cut(s) 415, 1145
MluCI AATT 6 cut(s) 141, 180, 267, 1070, 1198, 1218
MluNI TGGCCA 2 cut(s) 415, 1145
MlyI GAGTC 3 cut(s) 160, 230, 462
Mox20I TGGCCA 2 cut(s) 415, 1145
MscI TGGCCA 2 cut(s) 415, 1145
MseI TTAA 2 cut(s) 669, 1044
MslI CAYNNNNRTG 1 cut(s) 723
Msp20I TGGCCA 2 cut(s) 415, 1145
MspA1I CMGCKG 1 cut(s) 1034
MspI CCGG 4 cut(s) 6, 214, 774, 796
MspR9I CCNGG 7 cut(s) 6, 7, 215, 357, 775, 797, 1230
MunI CAATTG 1 cut(s) 1218
MvaI CCWGG 2 cut(s) 357, 1230
MwoI GCNNNNNNNGC 5 cut(s) 43, 52, 298, 350, 1234
NciI CCSGG 5 cut(s) 6, 7, 215, 775, 797
NcoI CCATGG 1 cut(s) 103
NdeII GATC 5 cut(s) 77, 85, 496, 679, 883
NlaIV GGNNCC 5 cut(s) 10, 27, 36, 932, 1379
NmuCI GTSAC 3 cut(s) 152, 583, 1193
NsbI TGCGCA 1 cut(s) 248
NspI RCATGY 2 cut(s) 434, 1171
NspV TTCGAA 1 cut(s) 811
PaeI GCATGC 1 cut(s) 434
PceI AGGCCT 1 cut(s) 612
PciSI GCTCTTC 2 cut(s) 750, 1181
PfeI GAWTC 2 cut(s) 235, 785
PflMI CCANNNNNTGG 1 cut(s) 411
PkrI GCNGC 2 cut(s) 464, 626
PleI GAGTC 3 cut(s) 159, 229, 461
PpsI GAGTC 3 cut(s) 159, 229, 461
PpuMI RGGWCCY 1 cut(s) 931
Psp124BI GAGCTC 2 cut(s) 264, 1091
Psp5II RGGWCCY 1 cut(s) 931
Psp6I CCWGG 2 cut(s) 355, 1228
PspFI CCCAGC 2 cut(s) 675, 996
PspGI CCWGG 2 cut(s) 355, 1228
PspN4I GGNNCC 5 cut(s) 10, 27, 36, 932, 1379
PspOMI GGGCCC 1 cut(s) 8
PspPI GGNCC 6 cut(s) 8, 9, 283, 931, 994, 1377
PspPPI RGGWCCY 1 cut(s) 931
PstNI CAGNNNCTG 1 cut(s) 46
PsuI RGATCY 2 cut(s) 679, 883
PvuII CAGCTG 1 cut(s) 1034
RsaI GTAC 4 cut(s) 480, 538, 1224, 1285
RsaNI GTAC 4 cut(s) 479, 537, 1223, 1284
RseI CAYNNNNRTG 1 cut(s) 723
SacI GAGCTC 2 cut(s) 264, 1091
SapI GCTCTTC 2 cut(s) 750, 1181
SaqAI TTAA 2 cut(s) 669, 1044
SatI GCNGC 2 cut(s) 463, 625
Sau3AI GATC 5 cut(s) 77, 85, 496, 679, 883
Sau96I GGNCC 6 cut(s) 8, 9, 283, 931, 994, 1377
SchI GAGTC 3 cut(s) 160, 230, 462
ScrFI CCNGG 7 cut(s) 6, 7, 215, 357, 775, 797, 1230
SduI GDGCHC 4 cut(s) 12, 264, 303, 1091
SfaNI GCATC 5 cut(s) 234, 810, 1009, 1031, 1037
SfcI CTRYAG 1 cut(s) 448
SfuI TTCGAA 1 cut(s) 811
SinI GGWCC 2 cut(s) 283, 931
SmaI CCCGGG 1 cut(s) 7
SmiMI CAYNNNNRTG 1 cut(s) 723
SpeI ACTAGT 1 cut(s) 664
SphI GCATGC 1 cut(s) 434
SrfI GCCCGGGC 1 cut(s) 7
Sse9I AATT 6 cut(s) 141, 180, 267, 1070, 1198, 1218
SseBI AGGCCT 1 cut(s) 612
SsiI CCGC 4 cut(s) 99, 462, 617, 1191
SspI AATATT 1 cut(s) 1042
SspMI CTAG 2 cut(s) 195, 665
SstI GAGCTC 2 cut(s) 264, 1091
StuI AGGCCT 1 cut(s) 612
StyD4I CCNGG 7 cut(s) 4, 5, 213, 355, 773, 795, 1228
StyI CCWWGG 4 cut(s) 15, 103, 295, 437
TaaI ACNGT 8 cut(s) 42, 305, 316, 536, 583, 856, 1244, 1398
TaiI ACGT 2 cut(s) 421, 784
TaqI TCGA 5 cut(s) 84, 597, 811, 874, 1393
TaqII GACCGA 1 cut(s) 271
TasI AATT 6 cut(s) 141, 180, 267, 1070, 1198, 1218
TatI WGTACW 1 cut(s) 1222
TauI GCSGC 1 cut(s) 465
TfiI GAWTC 2 cut(s) 235, 785
Tru1I TTAA 2 cut(s) 669, 1044
Tru9I TTAA 2 cut(s) 669, 1044
TscAI CASTG 6 cut(s) 36, 308, 526, 588, 1153, 1249
TseFI GTSAC 3 cut(s) 152, 583, 1193
TseI GCWGC 1 cut(s) 624
Tsp45I GTSAC 3 cut(s) 152, 583, 1193
TspDTI ATGAA 4 cut(s) 696, 737, 818, 1353
TspGWI ACGGA 2 cut(s) 692, 853
TspMI CCCGGG 1 cut(s) 5
TspRI CASTG 6 cut(s) 36, 308, 526, 588, 1153, 1249
Van91I CCANNNNNTGG 1 cut(s) 411
VpaK11BI GGWCC 2 cut(s) 283, 931
XapI RAATTY 4 cut(s) 141, 180, 267, 1070
XceI RCATGY 2 cut(s) 434, 1171
XcmI CCANNNNNNNNNTGG 3 cut(s) 19, 423, 507
XmaI CCCGGG 1 cut(s) 5
XspI CTAG 2 cut(s) 195, 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.