MD00G1184900.v1.1

Protein pleiotropic regulatory locus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0002996
Physical Location & Seq
Reverse (-)
43887856 .. 43893307
5452 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1184900.v1.1.491

Sequence Viewer

Length: 1512 bp
ATGCCCGGGCCCACCTTGGAGATGGAACCAGTGGAGCCACAGTCGCTGAAGAAGCTCAGTCTCAAATCCCTCAAACGATCCCTCGATCTCTTCTCCCCCGCCCATGGCGAACTCCCCCCTCCCGACCCCGAGAGTAAAAGAATTCGCATGAGTCACAAGATAAGCATGGAATATGGAGGAATTCAACCCACACCTAGCCAAAAACCACCTCGCCGGGCTGAGTCTGTTGCACCGGTAACTGGTTCTGAAGGGCCTTCCAATGCCCTTGCCCTTACAGATTCTAAGGATGCGCAGACGAGAGGAGCTCAAAATTCTTTGGTTGTCGGTCCTTCTGCACCAAGGGCACTGAATACTGTTGGTATTTCGGGTAAAGGCACTTTAGCTGTTTCTGCTCCAGGGTCATCTGAAAGGTTTTCAACATCGGCCATGATGGAAAGGATTTCCAGCAGATGGCCACGTCCTGATTGGCATGCTCCTTGGAAAATCTACAGAGTCATTAGCGGCCACTTGGGATGGGTACGAGCTATTGCCTTTGATCCAAGTAATAAATGGTTCTGCACTGGCTCAGCAGACCGTACTATCAAGATTTGGGATGTTGCAACTGGAACTCTACAACTGACAGTGACAGGACACATCGAGCAAATAAGAGGCCTTGCGGTTAGCAGCAGGCATACCTATATGTTCTCTGCTGGAGATGACAAACTAGTTAAATGCTGGGATCTTGAACAGAATAAGGTTATCCGTTCATATCATGGTCATCTAAGTGGTGTTTACTGCTTGGCTCTTCATCCCACTCTTGATGTTTTACTTACCGGGGGACGTGATTCTGTGTGCCGGGTATGTAATTATCTTATTAAAATTCAAATCCTGCATACTATAATGGAGGAAGCACTCTTGGGGATTGAGCAAAGTGATTGTTGTAATATTTATCAGAGTGGTTTGGGATATTCGAAGCAAGATGCAAGTTCATGCGTTGTCAGGCATGACAACACAGATCCACAAGTTGTTACTGGCTCTCACGACACAACCATCAAGTTCTGGGACCTTAGATATGGAAAAACAATGTCAACTCTGACGCACCATAAAAAGTCTGTGCGTGCAATGGCCCAGCATCCTAGAGTAAGTGCCAATTCATGTACTTTTATAGCCAAGGGAGGAAAGCGAACTTACCTGACTGACCAAAATCACAGGCTCAGTAGCATACATGATCGCCAACCAGGATTCAGAAGGATTCGTGTCATCGACATGATTGTGGTGAAATGGCCTCAGCATCCGAGTCAAGCTCCCACCATCTGCCAGTATCATCCTTCGCAAATTTTCATTCACATGCACGCACGCGCGCAAAAACACACCAACAAAAACTTTGATTTCCTCGTCCTCACTCGCAGCATCAACCTCACCATTCCAGTTATGCAAACTTTGTTCTTCCTCTGGCTTGTATGTCCAGTCCAATATCTTCAACACGTGATGGTCTTTCCATTTCCCCACAACCGAAATCAGCCTCCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
WD40

Protein Analysis

504

Amino Acids

56.29

Weight (kDa)

9.57

Isoelectric Point (pI)

52.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Gbeta PF25391 146 - 301 3.5e-15 G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd PF25047 150 - 281 3.6e-08 TEP-1 second beta-propeller
Beta-prop_WDR41 PF25178 157 - 276 2.4e-09 WDR41
Beta-prop_THOC3 PF25174 160 - 246 1.2e-15 THOC3 beta-propeller domain
WD40 PF00400 161 - 198 7.2e-11 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 162 - 280 1.8e-17 WDR3 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 163 - 241 3.5e-07 MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz PF24807 163 - 280 2.1e-14 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 165 - 280 3.5e-24 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 166 - 282 1.8e-25 WDR5 beta-propeller domain
WD40_Prp19 PF24814 167 - 280 1.6e-16 Prp19 WD40 domain
WDR55 PF24796 168 - 283 2e-09 WDR55
WD40_WDHD1_1st PF24817 173 - 249 9.3e-11 WDHD1 first WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 175 - 245 2.1e-06 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 180 - 368 2.1e-08 WDR36/Utp21 first beta-propeller
Beta-prop_THOC3 PF25174 226 - 279 6.1e-06 THOC3 beta-propeller domain
WD40 PF00400 245 - 280 7.6e-06 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 323 - 368 9.2e-06 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 332 - 372 1.2e-06 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 291
AccB7I CCANNNNNTGG 1 cut(s) 450
AccII CGCG 2 cut(s) 1338, 1340
AciI CCGC 3 cut(s) 99, 501, 656
AclWI GGATC 4 cut(s) 72, 530, 726, 989
AcoI YGGCCR 3 cut(s) 423, 452, 502
AcsI RAATTY 5 cut(s) 141, 180, 310, 858, 1314
AcuI CTGAAG 2 cut(s) 68, 267
AcvI CACGTG 1 cut(s) 1465
AfaI GTAC 3 cut(s) 519, 577, 1138
AfiI CCNNNNNNNGG 3 cut(s) 104, 239, 450
AflIII ACRYGT 1 cut(s) 1462
AgeI ACCGGT 1 cut(s) 232
AgsI TTSAA 5 cut(s) 185, 417, 725, 863, 1460
AhlI ACTAGT 1 cut(s) 703
AjiI CACGTC 2 cut(s) 458, 821
AjnI CCWGG 2 cut(s) 394, 1216
AluBI AGCT 5 cut(s) 55, 305, 383, 524, 1283
AluI AGCT 5 cut(s) 55, 305, 383, 524, 1283
Alw21I GWGCWC 1 cut(s) 307
Alw26I GTCTC 1 cut(s) 65
AlwI GGATC 4 cut(s) 72, 530, 726, 989
AlwNI CAGNNNCTG 1 cut(s) 46
Ama87I CYCGRG 2 cut(s) 5, 128
AoxI GGCC 8 cut(s) 8, 251, 423, 452, 502, 649, 1104, 1262
ApaI GGGCCC 1 cut(s) 12
ApeKI GCWGC 2 cut(s) 663, 1386
ApoI RAATTY 5 cut(s) 141, 180, 310, 858, 1314
AsiGI ACCGGT 1 cut(s) 232
AspLEI GCGC 3 cut(s) 292, 1340, 1342
AspS9I GGNCC 6 cut(s) 8, 9, 251, 326, 1042, 1105
AsuC2I CCSGG 5 cut(s) 6, 7, 215, 814, 836
AsuHPI GGTGA 2 cut(s) 1267, 1390
AsuII TTCGAA 1 cut(s) 950
AvaI CYCGRG 2 cut(s) 5, 128
AvaII GGWCC 2 cut(s) 326, 1042
BaeGI GKGCMC 2 cut(s) 12, 346
BalI TGGCCA 1 cut(s) 454
BanII GRGCYC 2 cut(s) 12, 307
BbrPI CACGTG 1 cut(s) 1465
Bbv12I GWGCWC 1 cut(s) 307
BbvCI CCTCAGC 1 cut(s) 1266
BbvI GCAGC 2 cut(s) 675, 1398
BccI CCATC 7 cut(s) 16, 424, 444, 507, 1037, 1298, 1462
BciT130I CCWGG 2 cut(s) 396, 1218
BcnI CCSGG 5 cut(s) 6, 7, 215, 814, 836
BcoDI GTCTC 1 cut(s) 65
BcuI ACTAGT 1 cut(s) 703
BfaI CTAG 3 cut(s) 195, 704, 1116
BfmI CTRYAG 1 cut(s) 487
BisI GCNGC 3 cut(s) 502, 664, 1387
BlpI GCTNAGC 1 cut(s) 565
BlsI GCNGC 3 cut(s) 503, 665, 1388
Bme1390I CCNGG 7 cut(s) 6, 7, 215, 396, 814, 836, 1218
Bme18I GGWCC 2 cut(s) 326, 1042
BmeT110I CYCGRG 2 cut(s) 5, 128
BmgBI CACGTC 2 cut(s) 458, 821
BmgT120I GGNCC 6 cut(s) 8, 9, 251, 326, 1042, 1105
BmiI GGNNCC 4 cut(s) 10, 27, 36, 1043
BmrFI CCNGG 7 cut(s) 6, 7, 215, 396, 814, 836, 1218
BmsI GCATC 5 cut(s) 277, 949, 1120, 1279, 1398
BplI GAGNNNNNCTC 4 cut(s) 289, 321, 1267, 1299
BpmI CTGGAG 2 cut(s) 378, 711
Bpu10I CCTNAGC 1 cut(s) 1266
Bpu1102I GCTNAGC 1 cut(s) 565
Bpu14I TTCGAA 1 cut(s) 950
BpuMI CCSGG 5 cut(s) 6, 7, 215, 814, 836
BsaAI YACGTR 1 cut(s) 1465
BsaJI CCNNGG 8 cut(s) 5, 15, 103, 338, 395, 476, 813, 1149
BsaWI WCCGGW 1 cut(s) 232
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bsc4I CCNNNNNNNGG 3 cut(s) 104, 239, 450
Bse118I RCCGGY 1 cut(s) 232
Bse1I ACTGG 8 cut(s) 29, 244, 565, 607, 1015, 1297, 1406, 1445
Bse3DI GCAATG 1 cut(s) 1107
BseBI CCWGG 2 cut(s) 396, 1218
BseDI CCNNGG 8 cut(s) 5, 15, 103, 338, 395, 476, 813, 1149
BseGI GGATG 7 cut(s) 292, 518, 598, 787, 1111, 1270, 1303
BseLI CCNNNNNNNGG 3 cut(s) 104, 239, 450
BseMI GCAATG 1 cut(s) 1107
BseMII CTCAG 5 cut(s) 70, 210, 579, 1207, 1280
BseNI ACTGG 8 cut(s) 29, 244, 565, 607, 1015, 1297, 1406, 1445
BsePI GCGCGC 1 cut(s) 1338
BseRI GAGGAG 1 cut(s) 315
BseSI GKGCMC 2 cut(s) 12, 346
BseXI GCAGC 2 cut(s) 675, 1398
BseYI CCCAGC 2 cut(s) 714, 1107
BsgI GTGCAG 2 cut(s) 318, 541
Bsh1236I CGCG 2 cut(s) 1338, 1340
BshFI GGCC 8 cut(s) 10, 253, 425, 454, 504, 651, 1106, 1264
BshTI ACCGGT 1 cut(s) 232
BsiHKAI GWGCWC 1 cut(s) 307
BsiHKCI CYCGRG 2 cut(s) 5, 128
BsiSI CCGG 5 cut(s) 6, 214, 233, 813, 835
BslFI GGGAC 2 cut(s) 831, 1055
BslI CCNNNNNNNGG 3 cut(s) 104, 239, 450
BsmAI GTCTC 1 cut(s) 65
BsmFI GGGAC 2 cut(s) 831, 1055
BsnI GGCC 8 cut(s) 10, 253, 425, 454, 504, 651, 1106, 1264
BsoBI CYCGRG 2 cut(s) 5, 128
Bsp119I TTCGAA 1 cut(s) 950
Bsp120I GGGCCC 1 cut(s) 8
Bsp1286I GDGCHC 3 cut(s) 12, 307, 346
Bsp143I GATC 6 cut(s) 77, 85, 535, 718, 994, 1207
Bsp1720I GCTNAGC 1 cut(s) 565
Bsp19I CCATGG 1 cut(s) 103
BspACI CCGC 3 cut(s) 99, 501, 656
BspANI GGCC 8 cut(s) 10, 253, 425, 454, 504, 651, 1106, 1264
BspCNI CTCAG 5 cut(s) 69, 211, 578, 1206, 1279
BspFNI CGCG 2 cut(s) 1338, 1340
BspLI GGNNCC 4 cut(s) 10, 27, 36, 1043
BspPI GGATC 4 cut(s) 72, 530, 726, 989
BspQI GCTCTTC 1 cut(s) 789
BspT104I TTCGAA 1 cut(s) 950
BsrDI GCAATG 1 cut(s) 1107
BsrFI RCCGGY 1 cut(s) 232
BsrI ACTGG 8 cut(s) 29, 244, 565, 607, 1015, 1297, 1406, 1445
BssAI RCCGGY 1 cut(s) 232
BssECI CCNNGG 8 cut(s) 5, 15, 103, 338, 395, 476, 813, 1149
BssHII GCGCGC 1 cut(s) 1338
BssMI GATC 6 cut(s) 77, 85, 535, 718, 994, 1207
BssT1I CCWWGG 5 cut(s) 15, 103, 338, 476, 1149
Bst2UI CCWGG 2 cut(s) 396, 1218
Bst4CI ACNGT 4 cut(s) 42, 355, 575, 622
Bst6I CTCTTC 2 cut(s) 95, 789
BstBAI YACGTR 1 cut(s) 1465
BstBI TTCGAA 1 cut(s) 950
BstC8I GCNNGC 6 cut(s) 471, 668, 1098, 1332, 1336, 1340
BstDEI CTNAG 8 cut(s) 56, 219, 282, 565, 761, 1046, 1193, 1266
BstDSI CCRYGG 1 cut(s) 103
BstF5I GGATG 7 cut(s) 292, 518, 598, 787, 1111, 1270, 1303
BstFNI CGCG 2 cut(s) 1338, 1340
BstHHI GCGC 3 cut(s) 292, 1340, 1342
BstKTI GATC 6 cut(s) 80, 88, 538, 721, 997, 1210
BstMAI GTCTC 1 cut(s) 65
BstMBI GATC 6 cut(s) 77, 85, 535, 718, 994, 1207
BstMWI GCNNNNNNNGC 4 cut(s) 43, 52, 341, 389
BstNI CCWGG 2 cut(s) 396, 1218
BstNSI RCATGY 2 cut(s) 473, 1330
BstSCI CCNGG 7 cut(s) 4, 5, 213, 394, 812, 834, 1216
BstSFI CTRYAG 1 cut(s) 487
BstSLI GKGCMC 2 cut(s) 12, 346
BstUI CGCG 2 cut(s) 1338, 1340
BstV1I GCAGC 2 cut(s) 675, 1398
BstX2I RGATCY 2 cut(s) 718, 994
BstYI RGATCY 2 cut(s) 718, 994
BsuRI GGCC 8 cut(s) 10, 253, 425, 454, 504, 651, 1106, 1264
BtgI CCRYGG 1 cut(s) 103
BtrI CACGTC 2 cut(s) 458, 821
BtsCI GGATG 7 cut(s) 292, 518, 598, 787, 1111, 1270, 1303
BtsIMutI CAGTG 4 cut(s) 36, 344, 558, 627
Cac8I GCNNGC 6 cut(s) 471, 668, 1098, 1332, 1336, 1340
CaiI CAGNNNCTG 1 cut(s) 46
CfoI GCGC 3 cut(s) 292, 1340, 1342
Cfr10I RCCGGY 1 cut(s) 232
Cfr13I GGNCC 6 cut(s) 8, 9, 251, 326, 1042, 1105
Cfr9I CCCGGG 1 cut(s) 5
CseI GACGC 1 cut(s) 1084
Csp6I GTAC 3 cut(s) 518, 576, 1137
CspAI ACCGGT 1 cut(s) 232
CviQI GTAC 3 cut(s) 518, 576, 1137
DdeI CTNAG 8 cut(s) 56, 219, 282, 565, 761, 1046, 1193, 1266
DpnI GATC 6 cut(s) 79, 87, 537, 720, 996, 1209
DpnII GATC 6 cut(s) 77, 85, 535, 718, 994, 1207
EaeI YGGCCR 3 cut(s) 423, 452, 502
Eam1104I CTCTTC 2 cut(s) 95, 789
EarI CTCTTC 2 cut(s) 95, 789
Ecl136II GAGCTC 1 cut(s) 305
Eco130I CCWWGG 5 cut(s) 15, 103, 338, 476, 1149
Eco147I AGGCCT 1 cut(s) 651
Eco24I GRGCYC 2 cut(s) 12, 307
Eco47I GGWCC 2 cut(s) 326, 1042
Eco53kI GAGCTC 1 cut(s) 305
Eco57I CTGAAG 2 cut(s) 68, 267
Eco72I CACGTG 1 cut(s) 1465
Eco88I CYCGRG 2 cut(s) 5, 128
EcoICRI GAGCTC 1 cut(s) 305
EcoO109I RGGNCCY 2 cut(s) 251, 1042
EcoRI GAATTC 2 cut(s) 141, 180
EcoRII CCWGG 2 cut(s) 394, 1216
EcoT14I CCWWGG 5 cut(s) 15, 103, 338, 476, 1149
EcoT38I GRGCYC 2 cut(s) 12, 307
ErhI CCWWGG 5 cut(s) 15, 103, 338, 476, 1149
FaqI GGGAC 2 cut(s) 831, 1055
FauI CCCGC 1 cut(s) 106
Fnu4HI GCNGC 3 cut(s) 502, 664, 1387
FokI GGATG 7 cut(s) 299, 525, 605, 774, 1098, 1257, 1290
FriOI GRGCYC 2 cut(s) 12, 307
Fsp4HI GCNGC 3 cut(s) 502, 664, 1387
FspBI CTAG 3 cut(s) 195, 704, 1116
FspI TGCGCA 1 cut(s) 291
GlaI GCGC 3 cut(s) 291, 1339, 1341
GluI GCNGC 3 cut(s) 502, 664, 1387
GsaI CCCAGC 2 cut(s) 718, 1111
GsuI CTGGAG 2 cut(s) 378, 711
HaeIII GGCC 8 cut(s) 10, 253, 425, 454, 504, 651, 1106, 1264
HapII CCGG 5 cut(s) 6, 214, 233, 813, 835
HgaI GACGC 1 cut(s) 1084
HhaI GCGC 3 cut(s) 292, 1340, 1342
Hin6I GCGC 3 cut(s) 290, 1338, 1340
HinP1I GCGC 3 cut(s) 290, 1338, 1340
HincII GTYRAC 1 cut(s) 1068
HindII GTYRAC 1 cut(s) 1068
HinfI GANTC 8 cut(s) 151, 221, 278, 492, 824, 1221, 1231, 1276
HpaII CCGG 5 cut(s) 6, 214, 233, 813, 835
HphI GGTGA 2 cut(s) 1267, 1390
Hpy166II GTNNAC 2 cut(s) 772, 1068
Hpy188I TCNGA 6 cut(s) 247, 406, 933, 1074, 1226, 1275
Hpy188III TCNNGA 6 cut(s) 122, 461, 583, 722, 797, 1019
Hpy8I GTNNAC 2 cut(s) 772, 1068
HpyAV CCTTC 5 cut(s) 242, 264, 339, 1221, 1317
HpyCH4III ACNGT 4 cut(s) 42, 355, 575, 622
HpyCH4IV ACGT 3 cut(s) 457, 820, 1464
HpyCH4V TGCA 9 cut(s) 230, 335, 558, 599, 871, 962, 1100, 1330, 1414
HpyF10VI GCNNNNNNNGC 4 cut(s) 43, 52, 341, 389
HpyF3I CTNAG 8 cut(s) 56, 219, 282, 565, 761, 1046, 1193, 1266
HpySE526I ACGT 3 cut(s) 457, 820, 1464
HspAI GCGC 3 cut(s) 290, 1338, 1340
Kzo9I GATC 6 cut(s) 77, 85, 535, 718, 994, 1207
LguI GCTCTTC 1 cut(s) 789
LmnI GCTCC 5 cut(s) 34, 302, 397, 478, 1288
Lsp1109I GCAGC 2 cut(s) 675, 1398
LweI GCATC 5 cut(s) 277, 949, 1120, 1279, 1398
MaeI CTAG 3 cut(s) 195, 704, 1116
MaeII ACGT 3 cut(s) 457, 820, 1464
MaeIII GTNAC 4 cut(s) 152, 235, 622, 1006
MalI GATC 6 cut(s) 79, 87, 537, 720, 996, 1209
MboI GATC 6 cut(s) 77, 85, 535, 718, 994, 1207
MboII GAAGA 5 cut(s) 61, 82, 776, 1417, 1448
MflI RGATCY 2 cut(s) 718, 994
MhlI GDGCHC 3 cut(s) 12, 307, 346
MlsI TGGCCA 1 cut(s) 454
MluCI AATT 7 cut(s) 141, 180, 310, 844, 858, 1129, 1314
MluNI TGGCCA 1 cut(s) 454
MlyI GAGTC 4 cut(s) 160, 230, 501, 1285
Mox20I TGGCCA 1 cut(s) 454
MscI TGGCCA 1 cut(s) 454
MseI TTAA 3 cut(s) 708, 855, 1510
MslI CAYNNNNRTG 4 cut(s) 762, 1244, 1250, 1325
Msp20I TGGCCA 1 cut(s) 454
MspI CCGG 5 cut(s) 6, 214, 233, 813, 835
MspR9I CCNGG 7 cut(s) 6, 7, 215, 396, 814, 836, 1218
MvaI CCWGG 2 cut(s) 396, 1218
MvnI CGCG 2 cut(s) 1338, 1340
MwoI GCNNNNNNNGC 4 cut(s) 43, 52, 341, 389
NciI CCSGG 5 cut(s) 6, 7, 215, 814, 836
NcoI CCATGG 1 cut(s) 103
NdeII GATC 6 cut(s) 77, 85, 535, 718, 994, 1207
NlaIV GGNNCC 4 cut(s) 10, 27, 36, 1043
NmuCI GTSAC 2 cut(s) 152, 622
NsbI TGCGCA 1 cut(s) 291
NspI RCATGY 2 cut(s) 473, 1330
NspV TTCGAA 1 cut(s) 950
PaeI GCATGC 1 cut(s) 473
PauI GCGCGC 1 cut(s) 1338
PceI AGGCCT 1 cut(s) 651
PciSI GCTCTTC 1 cut(s) 789
PfeI GAWTC 4 cut(s) 278, 824, 1221, 1231
PflMI CCANNNNNTGG 1 cut(s) 450
PinAI ACCGGT 1 cut(s) 232
PkrI GCNGC 3 cut(s) 503, 665, 1388
PleI GAGTC 4 cut(s) 159, 229, 500, 1284
PmaCI CACGTG 1 cut(s) 1465
PmlI CACGTG 1 cut(s) 1465
PpsI GAGTC 4 cut(s) 159, 229, 500, 1284
Ppu21I YACGTR 1 cut(s) 1465
PpuMI RGGWCCY 1 cut(s) 1042
Psp124BI GAGCTC 1 cut(s) 307
Psp5II RGGWCCY 1 cut(s) 1042
Psp6I CCWGG 2 cut(s) 394, 1216
PspCI CACGTG 1 cut(s) 1465
PspFI CCCAGC 2 cut(s) 714, 1107
PspGI CCWGG 2 cut(s) 394, 1216
PspN4I GGNNCC 4 cut(s) 10, 27, 36, 1043
PspOMI GGGCCC 1 cut(s) 8
PspPI GGNCC 6 cut(s) 8, 9, 251, 326, 1042, 1105
PspPPI RGGWCCY 1 cut(s) 1042
PstNI CAGNNNCTG 1 cut(s) 46
PsuI RGATCY 2 cut(s) 718, 994
PteI GCGCGC 1 cut(s) 1338
RsaI GTAC 3 cut(s) 519, 577, 1138
RsaNI GTAC 3 cut(s) 518, 576, 1137
RseI CAYNNNNRTG 4 cut(s) 762, 1244, 1250, 1325
SacI GAGCTC 1 cut(s) 307
SapI GCTCTTC 1 cut(s) 789
SaqAI TTAA 3 cut(s) 708, 855, 1510
SatI GCNGC 3 cut(s) 502, 664, 1387
Sau3AI GATC 6 cut(s) 77, 85, 535, 718, 994, 1207
Sau96I GGNCC 6 cut(s) 8, 9, 251, 326, 1042, 1105
SchI GAGTC 4 cut(s) 160, 230, 501, 1285
ScrFI CCNGG 7 cut(s) 6, 7, 215, 396, 814, 836, 1218
SduI GDGCHC 3 cut(s) 12, 307, 346
SfaNI GCATC 5 cut(s) 277, 949, 1120, 1279, 1398
SfcI CTRYAG 1 cut(s) 487
SfuI TTCGAA 1 cut(s) 950
SinI GGWCC 2 cut(s) 326, 1042
SmaI CCCGGG 1 cut(s) 7
SmiMI CAYNNNNRTG 4 cut(s) 762, 1244, 1250, 1325
SpeI ACTAGT 1 cut(s) 703
SphI GCATGC 1 cut(s) 473
SrfI GCCCGGGC 1 cut(s) 7
Sse9I AATT 7 cut(s) 141, 180, 310, 844, 858, 1129, 1314
SseBI AGGCCT 1 cut(s) 651
SsiI CCGC 3 cut(s) 99, 501, 656
SspI AATATT 1 cut(s) 925
SspMI CTAG 3 cut(s) 195, 704, 1116
SstI GAGCTC 1 cut(s) 307
StuI AGGCCT 1 cut(s) 651
StyD4I CCNGG 7 cut(s) 4, 5, 213, 394, 812, 834, 1216
StyI CCWWGG 5 cut(s) 15, 103, 338, 476, 1149
TaaI ACNGT 4 cut(s) 42, 355, 575, 622
TaiI ACGT 3 cut(s) 460, 823, 1467
TaqI TCGA 4 cut(s) 84, 636, 950, 1242
TaqII GACCGA 1 cut(s) 314
TasI AATT 7 cut(s) 141, 180, 310, 844, 858, 1129, 1314
TatI WGTACW 1 cut(s) 1136
TauI GCSGC 1 cut(s) 504
TfiI GAWTC 4 cut(s) 278, 824, 1221, 1231
Tru1I TTAA 3 cut(s) 708, 855, 1510
Tru9I TTAA 3 cut(s) 708, 855, 1510
TscAI CASTG 4 cut(s) 36, 351, 565, 627
TseFI GTSAC 2 cut(s) 152, 622
TseI GCWGC 2 cut(s) 663, 1386
Tsp45I GTSAC 2 cut(s) 152, 622
TspDTI ATGAA 5 cut(s) 735, 776, 957, 1122, 1309
TspGWI ACGGA 1 cut(s) 731
TspMI CCCGGG 1 cut(s) 5
TspRI CASTG 4 cut(s) 36, 351, 565, 627
Van91I CCANNNNNTGG 1 cut(s) 450
VpaK11BI GGWCC 2 cut(s) 326, 1042
XapI RAATTY 5 cut(s) 141, 180, 310, 858, 1314
XceI RCATGY 2 cut(s) 473, 1330
XcmI CCANNNNNNNNNTGG 3 cut(s) 19, 462, 546
XmaI CCCGGG 1 cut(s) 5
XspI CTAG 3 cut(s) 195, 704, 1116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.