pycom06g06670

Protein pleiotropic regulatory locus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0002996
Physical Location & Seq
Forward (+)
10690441 .. 10690887
447 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g06670.1

Sequence Viewer

Length: 447 bp
ATGGCTTTCCATCCGACTCTTGACGTTTTAGTCACTGGCGGACGAGATAGTTTATGTCGTGTTTGGGATATTCGTCTCAAGAATGTGCAAGTTCATGTCTTGACTGGACATGAAGATACGGTGTTCTCCCTTCTCACCCACCCAATGGATCCACAAGTTATTAGCGGGTCTCACGATACAACGATCAAGCTTTGGGATCTTAGAAGGGGAAAACCCTTTTCTACGTTAACGCATCATAAGAAATCGATGCGGGCACTGACACATCTTCCTAAGCTAGATCGGGGTAATTGCTTCACATCGGCTTCAACAAACACCATCAAAAAGTTCAACCTTCCAAGGGGTGAGTTTTTGCACAACATGCTCTCCCAACAGAAGTCCATTATCAACGCAATGGCCGTCAATGAAGATGGAGTTATGGCTACGGGAAGTGATAATGGTAGCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
WD40

Protein Analysis

149

Amino Acids

16.52

Weight (kDa)

9.34

Isoelectric Point (pI)

17.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 1 - 89 7e-16 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 2 - 131 4.7e-18 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 2 - 85 7.7e-09 WDHD1 first WD40 domain
Beta-prop_TEP1_2nd PF25047 2 - 86 1.6e-07 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 3 - 138 6.9e-17 THOC3 beta-propeller domain
WD40_Prp19 PF24814 4 - 146 2.1e-15 Prp19 WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 5 - 136 9.4e-06 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 7 - 147 3.7e-07 WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz PF24807 8 - 90 2.6e-12 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 8 - 87 1.4e-06 WDR3 second beta-propeller domain
EIF3I PF24805 8 - 79 3.3e-06 EIF3I
WDR55 PF24796 9 - 71 3.4e-06 WDR55
Beta-prop_EIPR1 PF23609 9 - 65 4.1e-06 EIPR1 beta-propeller
Beta-prop_EML PF23409 10 - 144 8.7e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40_Gbeta PF25391 10 - 115 1.6e-12 G protein beta WD-40 repeat protein
Beta-prop_CAF1B_HIR1 PF24105 29 - 120 9.4e-08 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 30 - 66 6.9e-09 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 29
AccB7I CCANNNNNTGG 1 cut(s) 145
AciI CCGC 3 cut(s) 39, 165, 250
AclWI GGATC 3 cut(s) 143, 156, 204
AcoI YGGCCR 1 cut(s) 393
AfiI CCNNNNNNNGG 2 cut(s) 145, 337
AgsI TTSAA 2 cut(s) 306, 328
AluBI AGCT 2 cut(s) 190, 274
AluI AGCT 2 cut(s) 190, 274
Alw26I GTCTC 2 cut(s) 80, 174
AlwI GGATC 3 cut(s) 143, 156, 204
AoxI GGCC 1 cut(s) 393
AsuHPI GGTGA 2 cut(s) 127, 353
BaeGI GKGCMC 1 cut(s) 256
BamHI GGATCC 1 cut(s) 148
BccI CCATC 3 cut(s) 18, 323, 401
BceAI ACGGC 1 cut(s) 380
BcoDI GTCTC 2 cut(s) 80, 174
BfaI CTAG 1 cut(s) 275
BmiI GGNNCC 1 cut(s) 150
BmsI GCATC 2 cut(s) 237, 241
Bpu10I CCTNAGC 1 cut(s) 270
BpuEI CTTGAG 1 cut(s) 62
Bsa29I ATCGAT 1 cut(s) 245
BsaI GGTCTC 1 cut(s) 174
BsaJI CCNNGG 1 cut(s) 335
BsaXI ACNNNNNCTCC 2 cut(s) 347, 377
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 337
Bse1I ACTGG 2 cut(s) 40, 109
Bse3DI GCAATG 1 cut(s) 396
BseCI ATCGAT 1 cut(s) 245
BseDI CCNNGG 1 cut(s) 335
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 2 cut(s) 145, 337
BseMI GCAATG 1 cut(s) 396
BseNI ACTGG 2 cut(s) 40, 109
BseSI GKGCMC 1 cut(s) 256
BshFI GGCC 1 cut(s) 395
BshVI ATCGAT 1 cut(s) 245
BslI CCNNNNNNNGG 2 cut(s) 145, 337
BsmAI GTCTC 2 cut(s) 80, 174
BsmBI CGTCTC 1 cut(s) 80
BsnI GGCC 1 cut(s) 395
Bso31I GGTCTC 1 cut(s) 174
Bsp1286I GDGCHC 1 cut(s) 256
Bsp143I GATC 4 cut(s) 148, 183, 196, 277
BspACI CCGC 3 cut(s) 39, 165, 250
BspANI GGCC 1 cut(s) 395
BspDI ATCGAT 1 cut(s) 245
BspLI GGNNCC 1 cut(s) 150
BspPI GGATC 3 cut(s) 143, 156, 204
BspTNI GGTCTC 1 cut(s) 174
BsrDI GCAATG 1 cut(s) 396
BsrI ACTGG 2 cut(s) 40, 109
BssECI CCNNGG 1 cut(s) 335
BssMI GATC 4 cut(s) 148, 183, 196, 277
BssT1I CCWWGG 1 cut(s) 335
Bst4CI ACNGT 1 cut(s) 121
BstAPI GCANNNNNTGC 1 cut(s) 358
BstC8I GCNNGC 1 cut(s) 252
BstDEI CTNAG 2 cut(s) 200, 270
BstF5I GGATG 1 cut(s) 10
BstKTI GATC 4 cut(s) 151, 186, 199, 280
BstMAI GTCTC 2 cut(s) 80, 174
BstMBI GATC 4 cut(s) 148, 183, 196, 277
BstMWI GCNNNNNNNGC 1 cut(s) 358
BstNSI RCATGY 2 cut(s) 361, 445
BstSLI GKGCMC 1 cut(s) 256
BstX2I RGATCY 2 cut(s) 148, 196
BstYI RGATCY 2 cut(s) 148, 196
Bsu15I ATCGAT 1 cut(s) 245
BsuRI GGCC 1 cut(s) 395
BsuTUI ATCGAT 1 cut(s) 245
BtsCI GGATG 1 cut(s) 10
BtsIMutI CAGTG 2 cut(s) 33, 254
Cac8I GCNNGC 1 cut(s) 252
ClaI ATCGAT 1 cut(s) 245
CviAII CATG 4 cut(s) 95, 110, 358, 442
CviJI RGCY 6 cut(s) 5, 190, 274, 302, 395, 419
CviKI_1 RGCY 6 cut(s) 5, 190, 274, 302, 395, 419
DdeI CTNAG 2 cut(s) 200, 270
DpnI GATC 4 cut(s) 150, 185, 198, 279
DpnII GATC 4 cut(s) 148, 183, 196, 277
DrdI GACNNNNNNGTC 1 cut(s) 29
DseDI GACNNNNNNGTC 1 cut(s) 29
EaeI YGGCCR 1 cut(s) 393
EciI GGCGGA 1 cut(s) 54
Eco130I CCWWGG 1 cut(s) 335
Eco31I GGTCTC 1 cut(s) 174
EcoT14I CCWWGG 1 cut(s) 335
ErhI CCWWGG 1 cut(s) 335
Esp3I CGTCTC 1 cut(s) 80
FaeI CATG 4 cut(s) 98, 113, 361, 445
FaiI YATR 7 cut(s) 55, 96, 111, 237, 359, 416, 443
FatI CATG 4 cut(s) 94, 109, 357, 441
FauI CCCGC 2 cut(s) 158, 243
FspBI CTAG 1 cut(s) 275
HaeIII GGCC 1 cut(s) 395
Hin1II CATG 4 cut(s) 98, 113, 361, 445
HincII GTYRAC 1 cut(s) 228
HindII GTYRAC 1 cut(s) 228
HindIII AAGCTT 1 cut(s) 188
HinfI GANTC 1 cut(s) 16
HpaI GTTAAC 1 cut(s) 228
HphI GGTGA 2 cut(s) 127, 353
Hpy166II GTNNAC 1 cut(s) 228
Hpy188I TCNGA 1 cut(s) 15
Hpy188III TCNNGA 4 cut(s) 20, 79, 100, 173
Hpy8I GTNNAC 1 cut(s) 228
HpyAV CCTTC 3 cut(s) 140, 198, 341
HpyCH4III ACNGT 1 cut(s) 121
HpyCH4IV ACGT 2 cut(s) 24, 224
HpyCH4V TGCA 2 cut(s) 88, 352
HpyF10VI GCNNNNNNNGC 1 cut(s) 358
HpyF3I CTNAG 2 cut(s) 200, 270
HpySE526I ACGT 2 cut(s) 24, 224
Hsp92II CATG 4 cut(s) 98, 113, 361, 445
KspAI GTTAAC 1 cut(s) 228
Kzo9I GATC 4 cut(s) 148, 183, 196, 277
LpnPI CCDG 2 cut(s) 21, 90
LweI GCATC 2 cut(s) 237, 241
MaeI CTAG 1 cut(s) 275
MaeII ACGT 2 cut(s) 24, 224
MaeIII GTNAC 1 cut(s) 31
MalI GATC 4 cut(s) 150, 185, 198, 279
MboI GATC 4 cut(s) 148, 183, 196, 277
MboII GAAGA 3 cut(s) 125, 257, 416
MflI RGATCY 2 cut(s) 148, 196
MhlI GDGCHC 1 cut(s) 256
MluCI AATT 1 cut(s) 286
MlyI GAGTC 1 cut(s) 10
MmeI TCCRAC 1 cut(s) 38
MseI TTAA 1 cut(s) 227
MwoI GCNNNNNNNGC 1 cut(s) 358
NdeII GATC 4 cut(s) 148, 183, 196, 277
NlaIII CATG 4 cut(s) 98, 113, 361, 445
NlaIV GGNNCC 1 cut(s) 150
NmuCI GTSAC 1 cut(s) 31
NspI RCATGY 2 cut(s) 361, 445
PcsI WCGNNNNNNNCGW 1 cut(s) 393
PflMI CCANNNNNTGG 1 cut(s) 145
PleI GAGTC 1 cut(s) 10
PpsI GAGTC 1 cut(s) 10
PspN4I GGNNCC 1 cut(s) 150
PsuI RGATCY 2 cut(s) 148, 196
SaqAI TTAA 1 cut(s) 227
Sau3AI GATC 4 cut(s) 148, 183, 196, 277
SchI GAGTC 1 cut(s) 10
SduI GDGCHC 1 cut(s) 256
SetI ASST 5 cut(s) 27, 192, 227, 276, 333
SfaNI GCATC 2 cut(s) 237, 241
SmlI CTYRAG 1 cut(s) 77
SmoI CTYRAG 1 cut(s) 77
Sse9I AATT 1 cut(s) 286
SsiI CCGC 3 cut(s) 39, 165, 250
SspMI CTAG 1 cut(s) 275
StyI CCWWGG 1 cut(s) 335
TaaI ACNGT 1 cut(s) 121
TaiI ACGT 2 cut(s) 27, 227
TaqI TCGA 1 cut(s) 245
TasI AATT 1 cut(s) 286
Tru1I TTAA 1 cut(s) 227
Tru9I TTAA 1 cut(s) 227
TscAI CASTG 2 cut(s) 40, 261
TseFI GTSAC 1 cut(s) 31
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 3 cut(s) 83, 126, 417
TspRI CASTG 2 cut(s) 40, 261
Van91I CCANNNNNTGG 1 cut(s) 145
XceI RCATGY 2 cut(s) 361, 445
XspI CTAG 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.