Rw4G020860

Protein pleiotropic regulatory locus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002996
Physical Location & Seq
Forward (+)
44962839 .. 44967594
4756 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G020860.1

Sequence Viewer

Length: 1428 bp
ATGCCTGGTCCCACCTTAGAGATGGAGCGAGTAGAGCCACAGTCGCTGAAGAAGCTCACTTCCAAATCCCTCAAGCGAGCTCTCGATCTCTTCTCCCCCCTTCATAGCCAATTAGCTCCTCCCGATCCACAGAGTAAAAAAATTCGCATGAGCCACAAGATAAGCGTCGAGTATGGAGGAATTGTACCCACTGCTAGTCAGAAACCCCCTCGCCCAGCTGATTCTGGTGCTCCGAATCGGGGACCATCGGGCCTTCGAATGCCCTTGCCCTTCCAGACGGAAGCAGCTCAAAATGCTTTGGTGGTTGGTCCATCTGCACCAAAGGCACCGAATAATATTGGAATTTCGGGTAAAGGCACTTTAGTGGTTTCTGCTCCAGGATCATATGAAAGGAACCCATCAACATCAGCTATAATGGAAAGAATTTCCAGTAGATGGCCACGTCCTGAGTGGCATGCTCCTTGGAAGATTTACAGGGTCATCAGCGGCCACTTGGGATGGGTACGCTCTATTGCTTTTGATCCAAGTAATAAGTGGTTTTGTACAGGCTCTGCAGATCGAACAATAAAGATATGGGATGTAGGAACCGGAACACTACAGCTTACACTGACAGGACATATTGAGCAAATACGAGGCCTTGCTGTTAGCAGCAGGCATACCTATATGTTCTCGGCTGGTGATGATAAACAAGTCAAATGCTGGGACCTTGAGCAGAACAAGGTTATCCGTTCATACCATGGTCATCTAAGTGGTGTTTACTGCTTGGCTCTTCATCCCACTCTCGATATATTACTAACTGGGGGTCGTGATTCAGTATGCCGGGTCTGGGATATTCGTAGCAAGATGCAAGCTCATGCATTGTCCGGGCATGATAACACGGTTTGCTCGGTTTTCACTCGGCCAACGGATCCACAAGTTGTTACTGGCTCTCACGACTCAACCATCAAGTTCTGGGACCTTCGATATGGAAAAACGATGTCAACCATCACACACCATAAGAAATCTGTACGTGCAATTGCGCAACATCCTAAAGAGCATGCTTTTGCATCAGCATCAGCCGATAATATTAAGAAATTCAGCCTTCCAAGAGGGGAATTTTTACACAACATGCTCTCTCAGCAGAAAACCATAATTAATGCGATGGCTATCAATGAGGAGGGAGTTATGGCTACTGGAGGTGACAATGGGAGCTTGTGGTTTTGGGATTGGAAGAGTGGTCACAATTTTCAGCAATCACAAACAATCGTACAGCCTGGTTCACTGGATAGTGAAGCTGGTATATATGCGCTTTCCTATGATGTTACTGGTACGAGGCTTGTTAGTTGTGAAGCTGACAAGACAATCAAAATGTGGAAACAAGATGAAAATGCTACTCCAGAAACTCATCCTATCAATTTCAAGCCCCCTAAAGATATCAGACGGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
WD40

Protein Analysis

475

Amino Acids

52.64

Weight (kDa)

9.49

Isoelectric Point (pI)

47.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR41 PF25178 152 - 299 1.4e-11 WDR41
Beta-prop_THOC3 PF25174 155 - 264 2.7e-18 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 156 - 277 1.9e-19 WDR3 second beta-propeller domain
WD40 PF00400 156 - 193 4.2e-11 WD domain, G-beta repeat
WD40_Gbeta PF25391 156 - 297 1.4e-18 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 156 - 296 1.1e-23 Prp19 WD40 domain
WD40_MABP1-WDR62_2nd PF24782 158 - 236 1.3e-06 MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz PF24807 158 - 268 8.5e-14 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 159 - 254 4.1e-07 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 160 - 278 1.6e-26 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 161 - 331 1.6e-40 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 168 - 244 3.9e-11 WDHD1 first WD40 domain
WDR55 PF24796 168 - 324 8.3e-17 WDR55
Beta-prop_SPT8 PF23798 169 - 272 1e-06 SPT8 beta propeller
Beta-prop_WDR90_POC16_2nd PF23393 170 - 240 3.7e-06 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 175 - 356 1.1e-15 WDR36/Utp21 first beta-propeller
Beta-prop_SCAP PF24017 181 - 331 8.1e-08 SCAP Beta-propeller
WD40 PF00400 198 - 235 2.7e-06 WD domain, G-beta repeat
EIF3I PF24805 201 - 281 1.4e-06 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 202 - 282 3e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 203 - 341 1e-11 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 204 - 356 2.4e-15 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 206 - 410 8.5e-27 THOC3 beta-propeller domain
WD40_RFWD3 PF23419 206 - 288 6.3e-06 E3 ubiquitin-protein ligase RFWD3 WD40 domain
Beta-prop_EIPR1 PF23609 208 - 318 9.6e-08 EIPR1 beta-propeller
WD40_CDC20-Fz PF24807 218 - 451 4.8e-23 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 238 - 355 3.1e-23 WDR3 first beta-propeller domain
WD40 PF00400 240 - 277 1.6e-08 WD domain, G-beta repeat
Beta-prop_EML PF23409 243 - 460 4.8e-16 Echinoderm microtubule-associated protein first beta-propeller
WD40_MABP1-WDR62_2nd PF24782 248 - 450 4.6e-07 MABP1/WDR62 second WD40 domain
WD40_Prp19 PF24814 250 - 451 2e-28 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 252 - 413 7.7e-14 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 264 - 457 8.3e-23 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 284 - 357 1.3e-07 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 285 - 319 2e-06 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 287 - 402 2.9e-10 WDR3 second beta-propeller domain
EIF3I PF24805 287 - 412 3.6e-06 EIF3I
Beta-prop_EML_2 PF23414 298 - 455 4.2e-08 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 302 - 405 1e-06 WDR90/POC16, second beta-propeller
WD40_Gbeta PF25391 303 - 451 2.7e-07 G protein beta WD-40 repeat protein
WDR55 PF24796 325 - 410 1.4e-07 WDR55
Beta-prop_WDR5 PF25175 328 - 457 8.4e-10 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1020
AccB1I GGYRCC 1 cut(s) 325
AccB7I CCANNNNNTGG 1 cut(s) 435
AciI CCGC 1 cut(s) 486
AclWI GGATC 5 cut(s) 119, 388, 515, 902, 915
AcoI YGGCCR 3 cut(s) 437, 487, 899
AcsI RAATTY 5 cut(s) 141, 342, 423, 1073, 1094
AcuI CTGAAG 1 cut(s) 68
AfaI GTAC 6 cut(s) 186, 504, 544, 1008, 1248, 1309
AfiI CCNNNNNNNGG 3 cut(s) 239, 435, 826
AgsI TTSAA 1 cut(s) 1399
AjiI CACGTC 1 cut(s) 443
AjnI CCWGG 3 cut(s) 4, 376, 1252
AleI CACNNNNGTG 1 cut(s) 362
Alw21I GWGCWC 2 cut(s) 82, 232
AlwI GGATC 5 cut(s) 119, 388, 515, 902, 915
AlwNI CAGNNNCTG 2 cut(s) 46, 551
AoxI GGCC 5 cut(s) 250, 437, 487, 634, 899
ApeKI GCWGC 2 cut(s) 284, 648
ApoI RAATTY 5 cut(s) 141, 342, 423, 1073, 1094
AseI ATTAAT 1 cut(s) 1134
AspLEI GCGC 2 cut(s) 1021, 1288
AspS9I GGNCC 6 cut(s) 8, 242, 250, 308, 703, 955
AsuC2I CCSGG 2 cut(s) 821, 865
AsuHPI GGTGA 2 cut(s) 689, 1190
AsuII TTCGAA 1 cut(s) 256
AvaII GGWCC 5 cut(s) 8, 242, 308, 703, 955
BalI TGGCCA 1 cut(s) 439
BamHI GGATCC 1 cut(s) 907
BanI GGYRCC 1 cut(s) 325
BanII GRGCYC 1 cut(s) 82
Bbv12I GWGCWC 2 cut(s) 82, 232
BbvI GCAGC 2 cut(s) 296, 660
BccI CCATC 9 cut(s) 16, 253, 319, 406, 429, 492, 950, 992, 1135
BciT130I CCWGG 3 cut(s) 6, 378, 1254
BcnI CCSGG 2 cut(s) 821, 865
BfaI CTAG 2 cut(s) 195, 1426
BfmI CTRYAG 2 cut(s) 552, 596
BisI GCNGC 3 cut(s) 285, 487, 649
BlsI GCNGC 3 cut(s) 286, 488, 650
Bme1390I CCNGG 5 cut(s) 6, 378, 821, 865, 1254
Bme18I GGWCC 5 cut(s) 8, 242, 308, 703, 955
BmgBI CACGTC 1 cut(s) 443
BmgT120I GGNCC 6 cut(s) 8, 242, 250, 308, 703, 955
BmiI GGNNCC 8 cut(s) 10, 243, 327, 395, 586, 704, 909, 956
BmrFI CCNGG 5 cut(s) 6, 378, 821, 865, 1254
BmrI ACTGGG 1 cut(s) 807
BmsI GCATC 3 cut(s) 834, 1055, 1061
BmuI ACTGGG 1 cut(s) 807
BpmI CTGGAG 3 cut(s) 360, 1194, 1359
Bpu14I TTCGAA 1 cut(s) 256
BpuEI CTTGAG 2 cut(s) 56, 728
BpuMI CCSGG 2 cut(s) 821, 865
BsaAI YACGTR 1 cut(s) 1010
BsaBI GATNNNNATC 1 cut(s) 1145
BsaJI CCNNGG 2 cut(s) 461, 736
BsaWI WCCGGW 1 cut(s) 587
BsaXI ACNNNNNCTCC 2 cut(s) 168, 198
Bsc4I CCNNNNNNNGG 3 cut(s) 239, 435, 826
Bse1I ACTGG 6 cut(s) 429, 802, 928, 1177, 1266, 1309
Bse8I GATNNNNATC 1 cut(s) 1145
BseBI CCWGG 3 cut(s) 6, 378, 1254
BseDI CCNNGG 2 cut(s) 461, 736
BseGI GGATG 5 cut(s) 503, 583, 772, 1024, 1384
BseJI GATNNNNATC 1 cut(s) 1145
BseLI CCNNNNNNNGG 3 cut(s) 239, 435, 826
BseMII CTCAG 2 cut(s) 438, 1130
BseNI ACTGG 6 cut(s) 429, 802, 928, 1177, 1266, 1309
BseRI GAGGAG 2 cut(s) 108, 1169
BseXI GCAGC 2 cut(s) 296, 660
BseYI CCCAGC 2 cut(s) 214, 699
BsgI GTGCAG 1 cut(s) 300
BshFI GGCC 5 cut(s) 252, 439, 489, 636, 901
BshNI GGYRCC 1 cut(s) 325
BsiHKAI GWGCWC 2 cut(s) 82, 232
BsiSI CCGG 3 cut(s) 588, 820, 864
BslFI GGGAC 3 cut(s) 255, 716, 968
BslI CCNNNNNNNGG 3 cut(s) 239, 435, 826
BsmFI GGGAC 3 cut(s) 255, 716, 968
BsmI GAATGC 1 cut(s) 264
BsnI GGCC 5 cut(s) 252, 439, 489, 636, 901
Bsp119I TTCGAA 1 cut(s) 256
Bsp1286I GDGCHC 2 cut(s) 82, 232
Bsp1407I TGTACA 1 cut(s) 542
Bsp143I GATC 6 cut(s) 85, 124, 380, 520, 556, 907
Bsp19I CCATGG 1 cut(s) 736
BspACI CCGC 1 cut(s) 486
BspANI GGCC 5 cut(s) 252, 439, 489, 636, 901
BspCNI CTCAG 2 cut(s) 439, 1129
BspLI GGNNCC 8 cut(s) 10, 243, 327, 395, 586, 704, 909, 956
BspMAI CTGCAG 1 cut(s) 556
BspPI GGATC 5 cut(s) 119, 388, 515, 902, 915
BspQI GCTCTTC 1 cut(s) 774
BspT104I TTCGAA 1 cut(s) 256
BspT107I GGYRCC 1 cut(s) 325
BsrGI TGTACA 1 cut(s) 542
BsrI ACTGG 6 cut(s) 429, 802, 928, 1177, 1266, 1309
BssECI CCNNGG 2 cut(s) 461, 736
BssMI GATC 6 cut(s) 85, 124, 380, 520, 556, 907
BssT1I CCWWGG 2 cut(s) 461, 736
Bst2UI CCWGG 3 cut(s) 6, 378, 1254
Bst4CI ACNGT 3 cut(s) 42, 880, 1422
Bst6I CTCTTC 3 cut(s) 95, 774, 1205
BstAUI TGTACA 1 cut(s) 542
BstBAI YACGTR 1 cut(s) 1010
BstBI TTCGAA 1 cut(s) 256
BstC8I GCNNGC 5 cut(s) 78, 456, 653, 849, 1038
BstDEI CTNAG 4 cut(s) 16, 447, 746, 1116
BstDSI CCRYGG 1 cut(s) 736
BstF5I GGATG 5 cut(s) 503, 583, 772, 1024, 1384
BstHHI GCGC 2 cut(s) 1021, 1288
BstKTI GATC 6 cut(s) 88, 127, 383, 523, 559, 910
BstMBI GATC 6 cut(s) 85, 124, 380, 520, 556, 907
BstMWI GCNNNNNNNGC 6 cut(s) 34, 43, 52, 293, 323, 1117
BstNI CCWGG 3 cut(s) 6, 378, 1254
BstNSI RCATGY 3 cut(s) 458, 1040, 1111
BstSCI CCNGG 5 cut(s) 4, 376, 819, 863, 1252
BstSFI CTRYAG 2 cut(s) 552, 596
BstV1I GCAGC 2 cut(s) 296, 660
BstX2I RGATCY 1 cut(s) 907
BstYI RGATCY 1 cut(s) 907
BsuRI GGCC 5 cut(s) 252, 439, 489, 636, 901
BtgI CCRYGG 1 cut(s) 736
BtgZI GCGATG 1 cut(s) 1154
BtrI CACGTC 1 cut(s) 443
BtsCI GGATG 5 cut(s) 503, 583, 772, 1024, 1384
BtsI GCAGTG 1 cut(s) 189
BtsIMutI CAGTG 3 cut(s) 189, 605, 1259
Cac8I GCNNGC 5 cut(s) 78, 456, 653, 849, 1038
CaiI CAGNNNCTG 2 cut(s) 46, 551
CfoI GCGC 2 cut(s) 1021, 1288
Cfr13I GGNCC 6 cut(s) 8, 242, 250, 308, 703, 955
CseI GACGC 1 cut(s) 154
Csp6I GTAC 6 cut(s) 185, 503, 543, 1007, 1247, 1308
CviAII CATG 7 cut(s) 148, 455, 737, 854, 869, 1037, 1108
CviQI GTAC 6 cut(s) 185, 503, 543, 1007, 1247, 1308
DdeI CTNAG 4 cut(s) 16, 447, 746, 1116
DpnI GATC 6 cut(s) 87, 126, 382, 522, 558, 909
DpnII GATC 6 cut(s) 85, 124, 380, 520, 556, 907
EaeI YGGCCR 3 cut(s) 437, 487, 899
Eam1104I CTCTTC 3 cut(s) 95, 774, 1205
EarI CTCTTC 3 cut(s) 95, 774, 1205
Ecl136II GAGCTC 1 cut(s) 80
Eco130I CCWWGG 2 cut(s) 461, 736
Eco147I AGGCCT 1 cut(s) 636
Eco24I GRGCYC 1 cut(s) 82
Eco32I GATATC 1 cut(s) 1414
Eco47I GGWCC 5 cut(s) 8, 242, 308, 703, 955
Eco53kI GAGCTC 1 cut(s) 80
Eco57I CTGAAG 1 cut(s) 68
EcoICRI GAGCTC 1 cut(s) 80
EcoO109I RGGNCCY 2 cut(s) 703, 955
EcoRII CCWGG 3 cut(s) 4, 376, 1252
EcoRV GATATC 1 cut(s) 1414
EcoT14I CCWWGG 2 cut(s) 461, 736
EcoT22I ATGCAT 1 cut(s) 859
EcoT38I GRGCYC 1 cut(s) 82
ErhI CCWWGG 2 cut(s) 461, 736
FaeI CATG 7 cut(s) 151, 458, 740, 857, 872, 1040, 1111
FaqI GGGAC 3 cut(s) 255, 716, 968
FatI CATG 7 cut(s) 147, 454, 736, 853, 868, 1036, 1107
FauNDI CATATG 1 cut(s) 385
Fnu4HI GCNGC 3 cut(s) 285, 487, 649
FokI GGATG 5 cut(s) 510, 590, 759, 1011, 1371
FriOI GRGCYC 1 cut(s) 82
Fsp4HI GCNGC 3 cut(s) 285, 487, 649
FspBI CTAG 2 cut(s) 195, 1426
FspI TGCGCA 1 cut(s) 1020
GlaI GCGC 2 cut(s) 1020, 1287
GluI GCNGC 3 cut(s) 285, 487, 649
GsaI CCCAGC 2 cut(s) 218, 703
GsuI CTGGAG 3 cut(s) 360, 1194, 1359
HaeIII GGCC 5 cut(s) 252, 439, 489, 636, 901
HapII CCGG 3 cut(s) 588, 820, 864
HgaI GACGC 1 cut(s) 154
HhaI GCGC 2 cut(s) 1021, 1288
Hin1II CATG 7 cut(s) 151, 458, 740, 857, 872, 1040, 1111
Hin6I GCGC 2 cut(s) 1019, 1286
HinP1I GCGC 2 cut(s) 1019, 1286
HincII GTYRAC 1 cut(s) 981
HindII GTYRAC 1 cut(s) 981
HinfI GANTC 4 cut(s) 221, 235, 809, 935
HpaII CCGG 3 cut(s) 588, 820, 864
HphI GGTGA 2 cut(s) 689, 1190
Hpy166II GTNNAC 3 cut(s) 757, 981, 1259
Hpy188I TCNGA 3 cut(s) 201, 234, 1418
Hpy188III TCNNGA 8 cut(s) 83, 122, 274, 446, 782, 806, 932, 1376
Hpy8I GTNNAC 3 cut(s) 757, 981, 1259
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 5 cut(s) 110, 263, 280, 968, 1091
HpyCH4III ACNGT 3 cut(s) 42, 880, 1422
HpyCH4IV ACGT 2 cut(s) 442, 1009
HpyCH4V TGCA 6 cut(s) 317, 554, 847, 857, 1013, 1046
HpyF10VI GCNNNNNNNGC 6 cut(s) 34, 43, 52, 293, 323, 1117
HpyF3I CTNAG 4 cut(s) 16, 447, 746, 1116
HpySE526I ACGT 2 cut(s) 442, 1009
Hsp92II CATG 7 cut(s) 151, 458, 740, 857, 872, 1040, 1111
HspAI GCGC 2 cut(s) 1019, 1286
Kzo9I GATC 6 cut(s) 85, 124, 380, 520, 556, 907
LguI GCTCTTC 1 cut(s) 774
LmnI GCTCC 6 cut(s) 25, 121, 235, 379, 463, 1188
Lsp1109I GCAGC 2 cut(s) 296, 660
LweI GCATC 3 cut(s) 834, 1055, 1061
MaeI CTAG 2 cut(s) 195, 1426
MaeII ACGT 2 cut(s) 442, 1009
MaeIII GTNAC 4 cut(s) 919, 1178, 1217, 1300
MalI GATC 6 cut(s) 87, 126, 382, 522, 558, 909
MboI GATC 6 cut(s) 85, 124, 380, 520, 556, 907
MboII GAAGA 5 cut(s) 61, 82, 478, 761, 1222
MfeI CAATTG 1 cut(s) 1014
MflI RGATCY 1 cut(s) 907
MhlI GDGCHC 2 cut(s) 82, 232
MlsI TGGCCA 1 cut(s) 439
MluNI TGGCCA 1 cut(s) 439
MlyI GAGTC 1 cut(s) 929
Mox20I TGGCCA 1 cut(s) 439
Mph1103I ATGCAT 1 cut(s) 859
MscI TGGCCA 1 cut(s) 439
MseI TTAA 2 cut(s) 1068, 1134
MslI CAYNNNNRTG 2 cut(s) 362, 747
Msp20I TGGCCA 1 cut(s) 439
MspA1I CMGCKG 2 cut(s) 218, 486
MspI CCGG 3 cut(s) 588, 820, 864
MspR9I CCNGG 5 cut(s) 6, 378, 821, 865, 1254
MunI CAATTG 1 cut(s) 1014
Mva1269I GAATGC 1 cut(s) 264
MvaI CCWGG 3 cut(s) 6, 378, 1254
MwoI GCNNNNNNNGC 6 cut(s) 34, 43, 52, 293, 323, 1117
NciI CCSGG 2 cut(s) 821, 865
NcoI CCATGG 1 cut(s) 736
NdeI CATATG 1 cut(s) 385
NdeII GATC 6 cut(s) 85, 124, 380, 520, 556, 907
NlaIII CATG 7 cut(s) 151, 458, 740, 857, 872, 1040, 1111
NlaIV GGNNCC 8 cut(s) 10, 243, 327, 395, 586, 704, 909, 956
NmeAIII GCCGAG 2 cut(s) 650, 877
NmuCI GTSAC 2 cut(s) 1178, 1217
NsbI TGCGCA 1 cut(s) 1020
NsiI ATGCAT 1 cut(s) 859
NspI RCATGY 3 cut(s) 458, 1040, 1111
NspV TTCGAA 1 cut(s) 256
OliI CACNNNNGTG 1 cut(s) 362
PaeI GCATGC 2 cut(s) 458, 1040
PceI AGGCCT 1 cut(s) 636
PciSI GCTCTTC 1 cut(s) 774
PctI GAATGC 1 cut(s) 264
PfeI GAWTC 3 cut(s) 221, 235, 809
PflMI CCANNNNNTGG 1 cut(s) 435
PfoI TCCNGGA 1 cut(s) 376
PkrI GCNGC 3 cut(s) 286, 488, 650
PleI GAGTC 1 cut(s) 929
PpsI GAGTC 1 cut(s) 929
Ppu21I YACGTR 1 cut(s) 1010
PpuMI RGGWCCY 2 cut(s) 703, 955
PshBI ATTAAT 1 cut(s) 1134
Psp124BI GAGCTC 1 cut(s) 82
Psp5II RGGWCCY 2 cut(s) 703, 955
Psp6I CCWGG 3 cut(s) 4, 376, 1252
PspFI CCCAGC 2 cut(s) 214, 699
PspGI CCWGG 3 cut(s) 4, 376, 1252
PspN4I GGNNCC 8 cut(s) 10, 243, 327, 395, 586, 704, 909, 956
PspPI GGNCC 6 cut(s) 8, 242, 250, 308, 703, 955
PspPPI RGGWCCY 2 cut(s) 703, 955
PstI CTGCAG 1 cut(s) 556
PstNI CAGNNNCTG 2 cut(s) 46, 551
PsuI RGATCY 1 cut(s) 907
PvuII CAGCTG 1 cut(s) 218
RsaI GTAC 6 cut(s) 186, 504, 544, 1008, 1248, 1309
RsaNI GTAC 6 cut(s) 185, 503, 543, 1007, 1247, 1308
RseI CAYNNNNRTG 2 cut(s) 362, 747
SacI GAGCTC 1 cut(s) 82
SapI GCTCTTC 1 cut(s) 774
SaqAI TTAA 2 cut(s) 1068, 1134
SatI GCNGC 3 cut(s) 285, 487, 649
Sau3AI GATC 6 cut(s) 85, 124, 380, 520, 556, 907
Sau96I GGNCC 6 cut(s) 8, 242, 250, 308, 703, 955
SchI GAGTC 1 cut(s) 929
ScrFI CCNGG 5 cut(s) 6, 378, 821, 865, 1254
SduI GDGCHC 2 cut(s) 82, 232
SfaNI GCATC 3 cut(s) 834, 1055, 1061
SfcI CTRYAG 2 cut(s) 552, 596
SfuI TTCGAA 1 cut(s) 256
SinI GGWCC 5 cut(s) 8, 242, 308, 703, 955
SmiMI CAYNNNNRTG 2 cut(s) 362, 747
SmlI CTYRAG 2 cut(s) 71, 707
SmoI CTYRAG 2 cut(s) 71, 707
SphI GCATGC 2 cut(s) 458, 1040
SseBI AGGCCT 1 cut(s) 636
SsiI CCGC 1 cut(s) 486
SspI AATATT 2 cut(s) 337, 1066
SspMI CTAG 2 cut(s) 195, 1426
SstI GAGCTC 1 cut(s) 82
StuI AGGCCT 1 cut(s) 636
StyD4I CCNGG 5 cut(s) 4, 376, 819, 863, 1252
StyI CCWWGG 2 cut(s) 461, 736
TaaI ACNGT 3 cut(s) 42, 880, 1422
TaiI ACGT 2 cut(s) 445, 1012
TaqI TCGA 6 cut(s) 84, 168, 256, 559, 783, 961
TatI WGTACW 1 cut(s) 542
TauI GCSGC 1 cut(s) 489
TfiI GAWTC 3 cut(s) 221, 235, 809
Tru1I TTAA 2 cut(s) 1068, 1134
Tru9I TTAA 2 cut(s) 1068, 1134
TscAI CASTG 3 cut(s) 196, 612, 1266
TseFI GTSAC 2 cut(s) 1178, 1217
TseI GCWGC 2 cut(s) 284, 648
Tsp45I GTSAC 2 cut(s) 1178, 1217
TspDTI ATGAA 5 cut(s) 92, 402, 720, 761, 1377
TspGWI ACGGA 3 cut(s) 293, 716, 920
TspRI CASTG 3 cut(s) 196, 612, 1266
Van91I CCANNNNNTGG 1 cut(s) 435
VpaK11BI GGWCC 5 cut(s) 8, 242, 308, 703, 955
VspI ATTAAT 1 cut(s) 1134
XapI RAATTY 5 cut(s) 141, 342, 423, 1073, 1094
XceI RCATGY 3 cut(s) 458, 1040, 1111
XcmI CCANNNNNNNNNTGG 3 cut(s) 19, 447, 531
XspI CTAG 2 cut(s) 195, 1426
Zsp2I ATGCAT 1 cut(s) 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.