RLG00000007041

Protein pleiotropic regulatory locus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0002996
Physical Location & Seq
Reverse (-)
12972698 .. 12973659
962 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007041

Sequence Viewer

Length: 777 bp
ATGTACAGGGTCATAAGTGGCCACTTGGGATCTGTAGGATCAATTGCTTTCGACCCAAGTAATAAATGGTTTTGCACTGGCTCAGCAGATCGCACAGTCAAGATATGGGATGTAGCAACCGGAAAGCTACAGCTTACACTGACAGGACATACTCTTCAAATTCGAGGCCTTGCCGTTAGCAGCAAGCATCCCTATATGTTCTCTGCTGGCGATGACAAAGAAGTTAAATGTTGGGACCTTACACGGAATAAGGTTTGGGATATTCGTACCCAAACGCATGTTCATGCATTCTTGGGGACGACAGCACAGTTTGCTCGGTTTTCACTAGACCAATCGATCCCCAAGTTGTCACTAGCTCTCACGACGCAACCATCAAGTTCTGGGACCCTTGAGGTGGAAAAGCAATTAGACAACATTAAGAAGTTCAACCTTCCGAGAGGGGAATTTTTACACAATATGCTAGCTCAGCAGAACACCATAGTTAACACAATGGCGGTCAATGGTGATGGAGTTATGGCCACCGGAGATGACAGTGGGAGTTTGTGGTTTTGGGATTGGGACAGTGGCCATAATTATCAGCAATCCCAAACAACTGTACAGCCAGGTTCACTGAATAGCGAAGCTGGTATTTGTGCTCTTTCTTATGATGTCACTGGCACAAGGCTAGTTACTTGTGGAGTTGACAAGACGATCAAAATGTGGAAACAAGACGAAAACGCCACACCAGAAACTCATCCTCTCAATTTCAGTCCGCCTAAAGATATCCGGCACCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
WD40

Protein Analysis

259

Amino Acids

28.59

Weight (kDa)

6.75

Isoelectric Point (pI)

34.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 2 - 94 2.3e-08 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 3 - 37 1.8e-10 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 3 - 83 4.6e-12 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 3 - 85 3e-10 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 4 - 87 4.3e-17 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 5 - 88 6e-19 WDR5 beta-propeller domain
WD40_Gbeta PF25391 5 - 83 3.3e-08 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 6 - 93 1.9e-15 THOC3 beta-propeller domain
EIF3I PF24805 6 - 43 5.1e-06 EIF3I
WD40_MABP1-WDR62_2nd PF24782 6 - 80 3e-06 MABP1/WDR62 second WD40 domain
WDR55 PF24796 8 - 84 5.1e-06 WDR55
WD40_WDHD1_1st PF24817 11 - 196 1.4e-12 WDHD1 first WD40 domain
WD40_Prp19 PF24814 12 - 86 6e-13 Prp19 WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 14 - 238 1.3e-08 WDR90/POC16, second beta-propeller
WD40 PF00400 41 - 79 4.7e-07 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_1st PF25171 84 - 236 5.6e-07 WDR36/Utp21 first beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 768
AciI CCGC 2 cut(s) 494, 752
AclWI GGATC 3 cut(s) 37, 46, 331
AcoI YGGCCR 3 cut(s) 19, 516, 565
AcsI RAATTY 2 cut(s) 159, 443
AfaI GTAC 3 cut(s) 5, 268, 597
AfiI CCNNNNNNNGG 1 cut(s) 394
AgsI TTSAA 2 cut(s) 158, 427
AjnI CCWGG 1 cut(s) 601
AjuI GAANNNNNNNTTGG 2 cut(s) 264, 296
AluBI AGCT 5 cut(s) 127, 133, 356, 464, 623
AluI AGCT 5 cut(s) 127, 133, 356, 464, 623
Alw21I GWGCWC 1 cut(s) 637
AlwI GGATC 3 cut(s) 37, 46, 331
AoxI GGCC 4 cut(s) 19, 166, 516, 565
ApeKI GCWGC 1 cut(s) 180
ApoI RAATTY 2 cut(s) 159, 443
AspS9I GGNCC 2 cut(s) 235, 384
AsuHPI GGTGA 1 cut(s) 515
AsuNHI GCTAGC 1 cut(s) 460
AvaII GGWCC 2 cut(s) 235, 384
BalI TGGCCA 3 cut(s) 21, 518, 567
BanI GGYRCC 1 cut(s) 768
Bbv12I GWGCWC 1 cut(s) 637
BbvI GCAGC 1 cut(s) 192
BccI CCATC 2 cut(s) 379, 500
BceAI ACGGC 1 cut(s) 158
BciT130I CCWGG 1 cut(s) 603
BfaI CTAG 5 cut(s) 326, 353, 461, 665, 775
BfmI CTRYAG 2 cut(s) 33, 128
BisI GCNGC 1 cut(s) 181
BlpI GCTNAGC 2 cut(s) 82, 465
BlsI GCNGC 1 cut(s) 182
Bme1390I CCNGG 1 cut(s) 603
Bme18I GGWCC 2 cut(s) 235, 384
BmgT120I GGNCC 2 cut(s) 235, 384
BmiI GGNNCC 4 cut(s) 236, 385, 386, 770
BmrFI CCNGG 1 cut(s) 603
BmsI GCATC 1 cut(s) 196
BmtI GCTAGC 1 cut(s) 464
Bpu1102I GCTNAGC 2 cut(s) 82, 465
BpuEI CTTGAG 1 cut(s) 410
Bsa29I ATCGAT 1 cut(s) 335
BsaWI WCCGGW 2 cut(s) 119, 521
Bsc4I CCNNNNNNNGG 1 cut(s) 394
Bse1I ACTGG 2 cut(s) 82, 658
BseBI CCWGG 1 cut(s) 603
BseCI ATCGAT 1 cut(s) 335
BseGI GGATG 3 cut(s) 115, 187, 733
BseLI CCNNNNNNNGG 1 cut(s) 394
BseMII CTCAG 2 cut(s) 96, 479
BseNI ACTGG 2 cut(s) 82, 658
BseXI GCAGC 1 cut(s) 192
BshFI GGCC 4 cut(s) 21, 168, 518, 567
BshNI GGYRCC 1 cut(s) 768
BshVI ATCGAT 1 cut(s) 335
BsiHKAI GWGCWC 1 cut(s) 637
BsiSI CCGG 3 cut(s) 120, 522, 766
BslFI GGGAC 4 cut(s) 248, 310, 397, 572
BslI CCNNNNNNNGG 1 cut(s) 394
BsmFI GGGAC 4 cut(s) 248, 310, 397, 572
BsmI GAATGC 1 cut(s) 287
BsnI GGCC 4 cut(s) 21, 168, 518, 567
Bsp1286I GDGCHC 1 cut(s) 637
Bsp1407I TGTACA 2 cut(s) 3, 595
Bsp143I GATC 5 cut(s) 29, 38, 88, 336, 690
Bsp1720I GCTNAGC 2 cut(s) 82, 465
BspACI CCGC 2 cut(s) 494, 752
BspANI GGCC 4 cut(s) 21, 168, 518, 567
BspCNI CTCAG 2 cut(s) 95, 478
BspDI ATCGAT 1 cut(s) 335
BspLI GGNNCC 4 cut(s) 236, 385, 386, 770
BspOI GCTAGC 1 cut(s) 464
BspPI GGATC 3 cut(s) 37, 46, 331
BspT107I GGYRCC 1 cut(s) 768
BsrGI TGTACA 2 cut(s) 3, 595
BsrI ACTGG 2 cut(s) 82, 658
BssMI GATC 5 cut(s) 29, 38, 88, 336, 690
Bst2UI CCWGG 1 cut(s) 603
Bst4CI ACNGT 5 cut(s) 97, 309, 533, 563, 595
Bst6I CTCTTC 1 cut(s) 159
BstAPI GCANNNNNTGC 1 cut(s) 311
BstAUI TGTACA 2 cut(s) 3, 595
BstC8I GCNNGC 3 cut(s) 185, 208, 462
BstDEI CTNAG 2 cut(s) 82, 465
BstF5I GGATG 3 cut(s) 115, 187, 733
BstKTI GATC 5 cut(s) 32, 41, 91, 339, 693
BstMBI GATC 5 cut(s) 29, 38, 88, 336, 690
BstMWI GCNNNNNNNGC 2 cut(s) 311, 466
BstNI CCWGG 1 cut(s) 603
BstNSI RCATGY 1 cut(s) 281
BstSCI CCNGG 1 cut(s) 601
BstSFI CTRYAG 2 cut(s) 33, 128
BstV1I GCAGC 1 cut(s) 192
BstX2I RGATCY 1 cut(s) 29
BstYI RGATCY 1 cut(s) 29
Bsu15I ATCGAT 1 cut(s) 335
BsuRI GGCC 4 cut(s) 21, 168, 518, 567
BsuTUI ATCGAT 1 cut(s) 335
BtgZI GCGATG 1 cut(s) 225
BtsCI GGATG 3 cut(s) 115, 187, 733
BtsIMutI CAGTG 6 cut(s) 75, 137, 538, 568, 608, 651
Cac8I GCNNGC 3 cut(s) 185, 208, 462
Cfr13I GGNCC 2 cut(s) 235, 384
ClaI ATCGAT 1 cut(s) 335
CseI GACGC 1 cut(s) 373
Csp6I GTAC 3 cut(s) 4, 267, 596
CviAII CATG 2 cut(s) 278, 284
CviQI GTAC 3 cut(s) 4, 267, 596
DdeI CTNAG 2 cut(s) 82, 465
DpnI GATC 5 cut(s) 31, 40, 90, 338, 692
DpnII GATC 5 cut(s) 29, 38, 88, 336, 690
EaeI YGGCCR 3 cut(s) 19, 516, 565
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
EciI GGCGGA 1 cut(s) 741
Eco147I AGGCCT 1 cut(s) 168
Eco32I GATATC 1 cut(s) 763
Eco47I GGWCC 2 cut(s) 235, 384
EcoO109I RGGNCCY 2 cut(s) 235, 384
EcoRII CCWGG 1 cut(s) 601
EcoRV GATATC 1 cut(s) 763
EcoT22I ATGCAT 1 cut(s) 289
FaeI CATG 2 cut(s) 281, 287
FaqI GGGAC 4 cut(s) 248, 310, 397, 572
FatI CATG 2 cut(s) 277, 283
Fnu4HI GCNGC 1 cut(s) 181
FokI GGATG 3 cut(s) 122, 174, 720
Fsp4HI GCNGC 1 cut(s) 181
FspBI CTAG 5 cut(s) 326, 353, 461, 665, 775
GluI GCNGC 1 cut(s) 181
HaeIII GGCC 4 cut(s) 21, 168, 518, 567
HapII CCGG 3 cut(s) 120, 522, 766
HgaI GACGC 1 cut(s) 373
Hin1II CATG 2 cut(s) 281, 287
HincII GTYRAC 2 cut(s) 484, 682
HindII GTYRAC 2 cut(s) 484, 682
HpaI GTTAAC 1 cut(s) 484
HpaII CCGG 3 cut(s) 120, 522, 766
HphI GGTGA 1 cut(s) 515
Hpy166II GTNNAC 3 cut(s) 484, 608, 682
Hpy188I TCNGA 1 cut(s) 435
Hpy188III TCNNGA 2 cut(s) 100, 361
Hpy8I GTNNAC 3 cut(s) 484, 608, 682
Hpy99I CGWCG 1 cut(s) 367
HpyAV CCTTC 1 cut(s) 440
HpyCH4III ACNGT 5 cut(s) 97, 309, 533, 563, 595
HpyCH4V TGCA 2 cut(s) 75, 287
HpyF10VI GCNNNNNNNGC 2 cut(s) 311, 466
HpyF3I CTNAG 2 cut(s) 82, 465
Hsp92II CATG 2 cut(s) 281, 287
KflI GGGWCCC 1 cut(s) 384
KspAI GTTAAC 1 cut(s) 484
Kzo9I GATC 5 cut(s) 29, 38, 88, 336, 690
Lsp1109I GCAGC 1 cut(s) 192
LweI GCATC 1 cut(s) 196
MaeI CTAG 5 cut(s) 326, 353, 461, 665, 775
MaeIII GTNAC 3 cut(s) 348, 649, 667
MalI GATC 5 cut(s) 31, 40, 90, 338, 692
MboI GATC 5 cut(s) 29, 38, 88, 336, 690
MboII GAAGA 1 cut(s) 146
MfeI CAATTG 1 cut(s) 42
MflI RGATCY 1 cut(s) 29
MhlI GDGCHC 1 cut(s) 637
MlsI TGGCCA 3 cut(s) 21, 518, 567
MluCI AATT 6 cut(s) 42, 159, 404, 443, 571, 742
MluNI TGGCCA 3 cut(s) 21, 518, 567
MnlI CCTC 4 cut(s) 158, 385, 431, 747
Mox20I TGGCCA 3 cut(s) 21, 518, 567
Mph1103I ATGCAT 1 cut(s) 289
MscI TGGCCA 3 cut(s) 21, 518, 567
MseI TTAA 3 cut(s) 225, 417, 483
MslI CAYNNNNRTG 1 cut(s) 282
Msp20I TGGCCA 3 cut(s) 21, 518, 567
MspI CCGG 3 cut(s) 120, 522, 766
MspR9I CCNGG 1 cut(s) 603
MunI CAATTG 1 cut(s) 42
Mva1269I GAATGC 1 cut(s) 287
MvaI CCWGG 1 cut(s) 603
MwoI GCNNNNNNNGC 2 cut(s) 311, 466
NdeII GATC 5 cut(s) 29, 38, 88, 336, 690
NheI GCTAGC 1 cut(s) 460
NlaIII CATG 2 cut(s) 281, 287
NlaIV GGNNCC 4 cut(s) 236, 385, 386, 770
NmuCI GTSAC 2 cut(s) 348, 649
NsiI ATGCAT 1 cut(s) 289
NspI RCATGY 1 cut(s) 281
PceI AGGCCT 1 cut(s) 168
PctI GAATGC 1 cut(s) 287
PkrI GCNGC 1 cut(s) 182
PpuMI RGGWCCY 2 cut(s) 235, 384
Psp5II RGGWCCY 2 cut(s) 235, 384
Psp6I CCWGG 1 cut(s) 601
PspGI CCWGG 1 cut(s) 601
PspN4I GGNNCC 4 cut(s) 236, 385, 386, 770
PspPI GGNCC 2 cut(s) 235, 384
PspPPI RGGWCCY 2 cut(s) 235, 384
PsuI RGATCY 1 cut(s) 29
RsaI GTAC 3 cut(s) 5, 268, 597
RsaNI GTAC 3 cut(s) 4, 267, 596
RseI CAYNNNNRTG 1 cut(s) 282
SaqAI TTAA 3 cut(s) 225, 417, 483
SatI GCNGC 1 cut(s) 181
Sau3AI GATC 5 cut(s) 29, 38, 88, 336, 690
Sau96I GGNCC 2 cut(s) 235, 384
ScrFI CCNGG 1 cut(s) 603
SduI GDGCHC 1 cut(s) 637
SfaNI GCATC 1 cut(s) 196
SfcI CTRYAG 2 cut(s) 33, 128
SinI GGWCC 2 cut(s) 235, 384
SmiMI CAYNNNNRTG 1 cut(s) 282
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
Sse9I AATT 6 cut(s) 42, 159, 404, 443, 571, 742
SseBI AGGCCT 1 cut(s) 168
SsiI CCGC 2 cut(s) 494, 752
SspMI CTAG 5 cut(s) 326, 353, 461, 665, 775
StuI AGGCCT 1 cut(s) 168
StyD4I CCNGG 1 cut(s) 601
TaaI ACNGT 5 cut(s) 97, 309, 533, 563, 595
TaqI TCGA 3 cut(s) 51, 163, 335
TasI AATT 6 cut(s) 42, 159, 404, 443, 571, 742
TatI WGTACW 2 cut(s) 3, 595
Tru1I TTAA 3 cut(s) 225, 417, 483
Tru9I TTAA 3 cut(s) 225, 417, 483
TscAI CASTG 6 cut(s) 82, 144, 538, 568, 615, 658
TseFI GTSAC 2 cut(s) 348, 649
TseI GCWGC 1 cut(s) 180
Tsp45I GTSAC 2 cut(s) 348, 649
TspDTI ATGAA 1 cut(s) 272
TspGWI ACGGA 1 cut(s) 259
TspRI CASTG 6 cut(s) 82, 144, 538, 568, 615, 658
VpaK11BI GGWCC 2 cut(s) 235, 384
XapI RAATTY 2 cut(s) 159, 443
XceI RCATGY 1 cut(s) 281
XcmI CCANNNNNNNNNTGG 1 cut(s) 63
XspI CTAG 5 cut(s) 326, 353, 461, 665, 775
Zsp2I ATGCAT 1 cut(s) 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.