FvH4_4g23450

Protein pleiotropic regulatory locus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002996
Physical Location & Seq
Forward (+)
25917790 .. 25919151
1362 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g23450.t1

Sequence Viewer

Length: 972 bp
ATGGAAGGACCTGATCCCAGTAGGTTGCCTCGTCCAGTTTGGCATCATCCATGGAAGATGTACAGGGTCATTAGTGGCCACTTGGGATCTGTAGGAGCGATTGCTTTCGACCCAAGTAATGAATGGTTTTGCACTGGCTCTGCAGATCGCACAATCAAGATATGGGATGTAGCAACCGGAAAGCTACAGCTCACACTGACAGGACATACCGGTCAAATTCGAGGCCTTGCCATTAGCAGCAAGCATACCTATATGTTCTCTGCTGGCGATGACAAAGAAGTTAAATGTTGGGACCTTACACAGAACAAGGTCATTCGTTCATATCCTGGTCATCTAAGTAGTGTTTACTGCTTGGCTACTCATCCCACCCTTGATGTTTTACTCACTGGGGGACGTGATTCGGTATGCCGGATTTGGGATATTCGAACTCAAACGCATGTTCATGCGTTCTTGGGGCACCACAGCACAGTTTGCTCGGTTTTCACTAGACCAACTGATCCCCAAGTTGTCACCGGCTCGCACGACGCGACCATCAAGTTCTGGGACCTTAGAGGTGGAAAAGCAATGTCAACTCTCACACACCATAAGAAATCGGTTCGTGCGATGGCGCTGCATCCTAAGGATCAGTATTGTTTTACGTCTGCGTCGGTGGACAACATTAAGAAGTTTAGCCTTCCAAGAGGGAAATTTTTACACAACATGCTAGCTCGGCAGAACACCATAGTTAACACAATGGCGGTCAACGGGGAGGGGATTATTGCCACCGGTGGTGACAATGGGAGTCTCTGGTTTTGGGATTGGGAGAGTGGCCACAGTTTTCAACAATCACAAACAACTGCACAACCAGGTTCACTTGATAGTGAAGCCGGTATGTGTGCTCTTTCTTACGATGTCACTGGCACAAGGCTTGTTACTTGTGGAGCTGACAAGACGATCAAAATGTGGAAGCAAGACGAAAACGCTACGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
WD40

Protein Analysis

324

Amino Acids

35.75

Weight (kDa)

8.73

Isoelectric Point (pI)

28.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 17 - 106 1.8e-10 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR41 PF25178 18 - 142 1.6e-09 WDR41
Beta-prop_THOC3 PF25174 18 - 127 1.1e-18 THOC3 beta-propeller domain
WD40 PF00400 19 - 56 3.5e-10 WD domain, G-beta repeat
WD40_MABP1-WDR62_2nd PF24782 19 - 99 2.7e-07 MABP1/WDR62 second WD40 domain
WD40_Gbeta PF25391 19 - 160 2.4e-19 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 19 - 140 1e-19 WDR3 second beta-propeller domain
WD40_Prp19 PF24814 20 - 159 6.3e-25 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 20 - 148 4.3e-19 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 21 - 119 1e-08 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 22 - 105 1.2e-18 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 24 - 316 1.9e-51 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 30 - 107 9.9e-13 WDHD1 first WD40 domain
WDR55 PF24796 31 - 187 2.4e-17 WDR55
Beta-prop_WDR90_POC16_2nd PF23393 32 - 103 8.6e-09 WDR90/POC16, second beta-propeller
Beta-prop_ACSF4 PF13570 35 - 277 6.7e-07 Beta-alanine-activating enzyme, beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 38 - 219 1.1e-17 WDR36/Utp21 first beta-propeller
WD40 PF00400 60 - 98 7.4e-08 WD domain, G-beta repeat
EIF3I PF24805 64 - 149 4.8e-08 EIF3I
Beta-prop_TEP1_2nd PF25047 65 - 204 1.4e-17 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 67 - 219 5.3e-17 WDR36/Utp21 second beta-propeller domain
WD40_RFWD3 PF23419 69 - 160 1.3e-07 E3 ubiquitin-protein ligase RFWD3 WD40 domain
WD40_MABP1-WDR62_2nd PF24782 73 - 272 2.9e-07 MABP1/WDR62 second WD40 domain
WD40_CDC20-Fz PF24807 93 - 214 7.3e-20 CDC20/Fizzy WD40 domain
WD40 PF00400 103 - 140 2.5e-08 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 104 - 224 2.2e-22 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 106 - 319 6.5e-16 Echinoderm microtubule-associated protein first beta-propeller
WD40_WDHD1_1st PF24817 114 - 278 2.4e-12 WDHD1 first WD40 domain
WD40_Prp19 PF24814 115 - 315 9.1e-26 Prp19 WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 124 - 229 7.6e-11 WDR90/POC16, second beta-propeller
Beta-prop_THOC3 PF25174 127 - 319 7.3e-24 THOC3 beta-propeller domain
WD40_Gbeta PF25391 128 - 225 9.5e-13 G protein beta WD-40 repeat protein
Beta-prop_CAF1B_HIR1 PF24105 147 - 234 3.5e-08 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 149 - 278 3.4e-07 EIF3I
Beta-prop_EML_2 PF23414 161 - 270 1.6e-07 Echinoderm microtubule-associated protein second beta-propeller
WDR55 PF24796 188 - 275 5.6e-08 WDR55
Beta-prop_WDR3_2nd PF25172 199 - 317 4.8e-07 WDR3 second beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 210
AccB1I GGYRCC 1 cut(s) 456
AccII CGCG 1 cut(s) 527
AciI CCGC 1 cut(s) 737
AclWI GGATC 4 cut(s) 8, 94, 491, 630
AcoI YGGCCR 2 cut(s) 76, 808
AcsI RAATTY 2 cut(s) 216, 686
AfaI GTAC 1 cut(s) 62
AfiI CCNNNNNNNGG 1 cut(s) 415
AgeI ACCGGT 2 cut(s) 209, 764
AgsI TTSAA 1 cut(s) 821
AjiI CACGTC 1 cut(s) 395
AjnI CCWGG 2 cut(s) 325, 844
AluBI AGCT 4 cut(s) 184, 190, 707, 923
AluI AGCT 4 cut(s) 184, 190, 707, 923
Alw21I GWGCWC 1 cut(s) 880
Alw26I GTCTC 1 cut(s) 788
AlwI GGATC 4 cut(s) 8, 94, 491, 630
AoxI GGCC 3 cut(s) 76, 223, 808
ApeKI GCWGC 2 cut(s) 237, 610
ApoI RAATTY 2 cut(s) 216, 686
AsiGI ACCGGT 2 cut(s) 209, 764
AspLEI GCGC 1 cut(s) 610
AspS9I GGNCC 3 cut(s) 8, 292, 544
AsuHPI GGTGA 2 cut(s) 502, 782
AsuII TTCGAA 1 cut(s) 424
AsuNHI GCTAGC 1 cut(s) 703
AvaII GGWCC 3 cut(s) 8, 292, 544
AxyI CCTNAGG 1 cut(s) 618
BaeGI GKGCMC 1 cut(s) 459
BalI TGGCCA 2 cut(s) 78, 810
BanI GGYRCC 1 cut(s) 456
Bbv12I GWGCWC 1 cut(s) 880
BbvI GCAGC 2 cut(s) 249, 597
BccI CCATC 2 cut(s) 539, 598
BcgI CGANNNNNNTGC 2 cut(s) 592, 626
BciT130I CCWGG 2 cut(s) 327, 846
BcoDI GTCTC 1 cut(s) 788
BfaI CTAG 2 cut(s) 486, 704
BfmI CTRYAG 3 cut(s) 90, 141, 185
BfoI RGCGCY 1 cut(s) 611
BisI GCNGC 2 cut(s) 238, 611
BlsI GCNGC 2 cut(s) 239, 612
Bme1390I CCNGG 2 cut(s) 327, 846
Bme18I GGWCC 3 cut(s) 8, 292, 544
BmgBI CACGTC 1 cut(s) 395
BmgT120I GGNCC 3 cut(s) 8, 292, 544
BmiI GGNNCC 3 cut(s) 293, 458, 545
BmrFI CCNGG 2 cut(s) 327, 846
BmrI ACTGGG 2 cut(s) 12, 396
BmsI GCATC 2 cut(s) 52, 622
BmtI GCTAGC 1 cut(s) 707
BmuI ACTGGG 2 cut(s) 12, 396
Bpu14I TTCGAA 1 cut(s) 424
BsaJI CCNNGG 1 cut(s) 50
BsaWI WCCGGW 3 cut(s) 176, 209, 764
Bsc4I CCNNNNNNNGG 1 cut(s) 415
Bse118I RCCGGY 4 cut(s) 209, 512, 764, 866
Bse1I ACTGG 5 cut(s) 18, 35, 139, 391, 901
Bse21I CCTNAGG 1 cut(s) 618
Bse3DI GCAATG 1 cut(s) 570
BseBI CCWGG 2 cut(s) 327, 846
BseDI CCNNGG 1 cut(s) 50
BseGI GGATG 4 cut(s) 46, 172, 361, 613
BseLI CCNNNNNNNGG 1 cut(s) 415
BseMI GCAATG 1 cut(s) 570
BseNI ACTGG 5 cut(s) 18, 35, 139, 391, 901
BseSI GKGCMC 1 cut(s) 459
BseXI GCAGC 2 cut(s) 249, 597
BsgI GTGCAG 1 cut(s) 822
Bsh1236I CGCG 1 cut(s) 527
BshFI GGCC 3 cut(s) 78, 225, 810
BshNI GGYRCC 1 cut(s) 456
BshTI ACCGGT 2 cut(s) 209, 764
BsiHKAI GWGCWC 1 cut(s) 880
BsiSI CCGG 6 cut(s) 177, 210, 409, 513, 765, 867
BslFI GGGAC 3 cut(s) 305, 405, 557
BslI CCNNNNNNNGG 1 cut(s) 415
BsmAI GTCTC 1 cut(s) 788
BsmFI GGGAC 3 cut(s) 305, 405, 557
BsnI GGCC 3 cut(s) 78, 225, 810
Bsp119I TTCGAA 1 cut(s) 424
Bsp1286I GDGCHC 2 cut(s) 459, 880
Bsp1407I TGTACA 1 cut(s) 60
Bsp143I GATC 6 cut(s) 13, 86, 145, 496, 622, 933
Bsp19I CCATGG 1 cut(s) 50
BspACI CCGC 1 cut(s) 737
BspANI GGCC 3 cut(s) 78, 225, 810
BspFNI CGCG 1 cut(s) 527
BspLI GGNNCC 3 cut(s) 293, 458, 545
BspMAI CTGCAG 1 cut(s) 145
BspOI GCTAGC 1 cut(s) 707
BspPI GGATC 4 cut(s) 8, 94, 491, 630
BspT104I TTCGAA 1 cut(s) 424
BspT107I GGYRCC 1 cut(s) 456
BsrDI GCAATG 1 cut(s) 570
BsrFI RCCGGY 4 cut(s) 209, 512, 764, 866
BsrGI TGTACA 1 cut(s) 60
BsrI ACTGG 5 cut(s) 18, 35, 139, 391, 901
BssAI RCCGGY 4 cut(s) 209, 512, 764, 866
BssECI CCNNGG 1 cut(s) 50
BssMI GATC 6 cut(s) 13, 86, 145, 496, 622, 933
BssT1I CCWWGG 1 cut(s) 50
Bst2UI CCWGG 2 cut(s) 327, 846
Bst4CI ACNGT 2 cut(s) 469, 815
BstAPI GCANNNNNTGC 1 cut(s) 471
BstAUI TGTACA 1 cut(s) 60
BstBI TTCGAA 1 cut(s) 424
BstC8I GCNNGC 4 cut(s) 242, 265, 518, 705
BstDEI CTNAG 3 cut(s) 335, 548, 618
BstDSI CCRYGG 1 cut(s) 50
BstF5I GGATG 4 cut(s) 46, 172, 361, 613
BstFNI CGCG 1 cut(s) 527
BstH2I RGCGCY 1 cut(s) 611
BstHHI GCGC 1 cut(s) 610
BstKTI GATC 6 cut(s) 16, 89, 148, 499, 625, 936
BstMAI GTCTC 1 cut(s) 788
BstMBI GATC 6 cut(s) 13, 86, 145, 496, 622, 933
BstMWI GCNNNNNNNGC 2 cut(s) 471, 709
BstNI CCWGG 2 cut(s) 327, 846
BstNSI RCATGY 2 cut(s) 440, 703
BstSCI CCNGG 2 cut(s) 325, 844
BstSFI CTRYAG 3 cut(s) 90, 141, 185
BstSLI GKGCMC 1 cut(s) 459
BstUI CGCG 1 cut(s) 527
BstV1I GCAGC 2 cut(s) 249, 597
BstX2I RGATCY 1 cut(s) 86
BstYI RGATCY 1 cut(s) 86
Bsu36I CCTNAGG 1 cut(s) 618
BsuRI GGCC 3 cut(s) 78, 225, 810
BtgI CCRYGG 1 cut(s) 50
BtgZI GCGATG 2 cut(s) 282, 617
BtrI CACGTC 1 cut(s) 395
BtsCI GGATG 4 cut(s) 46, 172, 361, 613
BtsIMutI CAGTG 4 cut(s) 132, 194, 384, 894
Cac8I GCNNGC 4 cut(s) 242, 265, 518, 705
CfoI GCGC 1 cut(s) 610
Cfr10I RCCGGY 4 cut(s) 209, 512, 764, 866
Cfr13I GGNCC 3 cut(s) 8, 292, 544
CseI GACGC 2 cut(s) 533, 633
CsiI ACCWGGT 1 cut(s) 844
Csp6I GTAC 1 cut(s) 61
CspAI ACCGGT 2 cut(s) 209, 764
CviAII CATG 4 cut(s) 51, 437, 443, 700
CviQI GTAC 1 cut(s) 61
DdeI CTNAG 3 cut(s) 335, 548, 618
DpnI GATC 6 cut(s) 15, 88, 147, 498, 624, 935
DpnII GATC 6 cut(s) 13, 86, 145, 496, 622, 933
DrdI GACNNNNNNGTC 1 cut(s) 210
DseDI GACNNNNNNGTC 1 cut(s) 210
EaeI YGGCCR 2 cut(s) 76, 808
Eco130I CCWWGG 1 cut(s) 50
Eco147I AGGCCT 1 cut(s) 225
Eco47I GGWCC 3 cut(s) 8, 292, 544
Eco81I CCTNAGG 1 cut(s) 618
EcoO109I RGGNCCY 3 cut(s) 8, 292, 544
EcoRII CCWGG 2 cut(s) 325, 844
EcoT14I CCWWGG 1 cut(s) 50
ErhI CCWWGG 1 cut(s) 50
FaeI CATG 4 cut(s) 54, 440, 446, 703
FaqI GGGAC 3 cut(s) 305, 405, 557
FatI CATG 4 cut(s) 50, 436, 442, 699
Fnu4HI GCNGC 2 cut(s) 238, 611
FokI GGATG 4 cut(s) 33, 179, 348, 600
Fsp4HI GCNGC 2 cut(s) 238, 611
FspBI CTAG 2 cut(s) 486, 704
GlaI GCGC 1 cut(s) 609
GluI GCNGC 2 cut(s) 238, 611
HaeII RGCGCY 1 cut(s) 611
HaeIII GGCC 3 cut(s) 78, 225, 810
HapII CCGG 6 cut(s) 177, 210, 409, 513, 765, 867
HgaI GACGC 2 cut(s) 533, 633
HhaI GCGC 1 cut(s) 610
Hin1II CATG 4 cut(s) 54, 440, 446, 703
Hin6I GCGC 1 cut(s) 608
HinP1I GCGC 1 cut(s) 608
HincII GTYRAC 3 cut(s) 570, 727, 742
HindII GTYRAC 3 cut(s) 570, 727, 742
HinfI GANTC 2 cut(s) 398, 781
HpaI GTTAAC 1 cut(s) 727
HpaII CCGG 6 cut(s) 177, 210, 409, 513, 765, 867
HphI GGTGA 2 cut(s) 502, 782
Hpy166II GTNNAC 6 cut(s) 346, 570, 652, 727, 742, 851
Hpy188III TCNNGA 1 cut(s) 157
Hpy8I GTNNAC 6 cut(s) 346, 570, 652, 727, 742, 851
Hpy99I CGWCG 2 cut(s) 527, 649
HpyAV CCTTC 1 cut(s) 683
HpyCH4III ACNGT 2 cut(s) 469, 815
HpyCH4IV ACGT 2 cut(s) 394, 638
HpyCH4V TGCA 4 cut(s) 132, 143, 613, 839
HpyF10VI GCNNNNNNNGC 2 cut(s) 471, 709
HpyF3I CTNAG 3 cut(s) 335, 548, 618
HpySE526I ACGT 2 cut(s) 394, 638
Hsp92II CATG 4 cut(s) 54, 440, 446, 703
HspAI GCGC 1 cut(s) 608
KspAI GTTAAC 1 cut(s) 727
Kzo9I GATC 6 cut(s) 13, 86, 145, 496, 622, 933
LmnI GCTCC 2 cut(s) 95, 920
Lsp1109I GCAGC 2 cut(s) 249, 597
LweI GCATC 2 cut(s) 52, 622
MabI ACCWGGT 1 cut(s) 844
MaeI CTAG 2 cut(s) 486, 704
MaeII ACGT 2 cut(s) 394, 638
MaeIII GTNAC 4 cut(s) 508, 770, 892, 910
MalI GATC 6 cut(s) 15, 88, 147, 498, 624, 935
MboI GATC 6 cut(s) 13, 86, 145, 496, 622, 933
MboII GAAGA 1 cut(s) 67
MflI RGATCY 1 cut(s) 86
MhlI GDGCHC 2 cut(s) 459, 880
MlsI TGGCCA 2 cut(s) 78, 810
MluCI AATT 2 cut(s) 216, 686
MluNI TGGCCA 2 cut(s) 78, 810
MlyI GAGTC 1 cut(s) 790
MnlI CCTC 5 cut(s) 39, 215, 545, 674, 742
Mox20I TGGCCA 2 cut(s) 78, 810
MscI TGGCCA 2 cut(s) 78, 810
MseI TTAA 3 cut(s) 282, 660, 726
MslI CAYNNNNRTG 1 cut(s) 441
Msp20I TGGCCA 2 cut(s) 78, 810
MspI CCGG 6 cut(s) 177, 210, 409, 513, 765, 867
MspR9I CCNGG 2 cut(s) 327, 846
MvaI CCWGG 2 cut(s) 327, 846
MvnI CGCG 1 cut(s) 527
MwoI GCNNNNNNNGC 2 cut(s) 471, 709
NcoI CCATGG 1 cut(s) 50
NdeII GATC 6 cut(s) 13, 86, 145, 496, 622, 933
NheI GCTAGC 1 cut(s) 703
NlaIII CATG 4 cut(s) 54, 440, 446, 703
NlaIV GGNNCC 3 cut(s) 293, 458, 545
NmeAIII GCCGAG 1 cut(s) 688
NmuCI GTSAC 3 cut(s) 508, 770, 892
NspI RCATGY 2 cut(s) 440, 703
NspV TTCGAA 1 cut(s) 424
PceI AGGCCT 1 cut(s) 225
PcsI WCGNNNNNNNCGW 2 cut(s) 524, 599
PfeI GAWTC 1 cut(s) 398
PinAI ACCGGT 2 cut(s) 209, 764
PkrI GCNGC 2 cut(s) 239, 612
PleI GAGTC 1 cut(s) 789
PpsI GAGTC 1 cut(s) 789
PpuMI RGGWCCY 3 cut(s) 8, 292, 544
Psp5II RGGWCCY 3 cut(s) 8, 292, 544
Psp6I CCWGG 2 cut(s) 325, 844
PspGI CCWGG 2 cut(s) 325, 844
PspN4I GGNNCC 3 cut(s) 293, 458, 545
PspPI GGNCC 3 cut(s) 8, 292, 544
PspPPI RGGWCCY 3 cut(s) 8, 292, 544
PstI CTGCAG 1 cut(s) 145
PsuI RGATCY 1 cut(s) 86
RsaI GTAC 1 cut(s) 62
RsaNI GTAC 1 cut(s) 61
RseI CAYNNNNRTG 1 cut(s) 441
SaqAI TTAA 3 cut(s) 282, 660, 726
SatI GCNGC 2 cut(s) 238, 611
Sau3AI GATC 6 cut(s) 13, 86, 145, 496, 622, 933
Sau96I GGNCC 3 cut(s) 8, 292, 544
SchI GAGTC 1 cut(s) 790
ScrFI CCNGG 2 cut(s) 327, 846
SduI GDGCHC 2 cut(s) 459, 880
SexAI ACCWGGT 1 cut(s) 844
SfaNI GCATC 2 cut(s) 52, 622
SfcI CTRYAG 3 cut(s) 90, 141, 185
SfuI TTCGAA 1 cut(s) 424
SgrAI CRCCGGYG 1 cut(s) 764
SinI GGWCC 3 cut(s) 8, 292, 544
SmiMI CAYNNNNRTG 1 cut(s) 441
Sse9I AATT 2 cut(s) 216, 686
SseBI AGGCCT 1 cut(s) 225
SsiI CCGC 1 cut(s) 737
SspMI CTAG 2 cut(s) 486, 704
StuI AGGCCT 1 cut(s) 225
StyD4I CCNGG 2 cut(s) 325, 844
StyI CCWWGG 1 cut(s) 50
TaaI ACNGT 2 cut(s) 469, 815
TaiI ACGT 2 cut(s) 397, 641
TaqI TCGA 3 cut(s) 108, 220, 424
TasI AATT 2 cut(s) 216, 686
TatI WGTACW 1 cut(s) 60
TfiI GAWTC 1 cut(s) 398
Tru1I TTAA 3 cut(s) 282, 660, 726
Tru9I TTAA 3 cut(s) 282, 660, 726
TscAI CASTG 4 cut(s) 139, 201, 391, 901
TseFI GTSAC 3 cut(s) 508, 770, 892
TseI GCWGC 2 cut(s) 237, 610
Tsp45I GTSAC 3 cut(s) 508, 770, 892
TspDTI ATGAA 3 cut(s) 135, 309, 431
TspRI CASTG 4 cut(s) 139, 201, 391, 901
VpaK11BI GGWCC 3 cut(s) 8, 292, 544
XapI RAATTY 2 cut(s) 216, 686
XceI RCATGY 2 cut(s) 440, 703
XcmI CCANNNNNNNNNTGG 1 cut(s) 120
XspI CTAG 2 cut(s) 486, 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.