Rroxscaffold_5G00371580

Protein pleiotropic regulatory locus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002996
Physical Location & Seq
Forward (+)
53159861 .. 53160823
963 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00371580.1

Sequence Viewer

Length: 963 bp
ATGTACAGGGTGATTAGTGGCCACTTGGGATCTGTAGGATTGATTGCTTTCGACCCAAGTAATAAATGGTTTTGCACTGGCTCAGTAGATCGCACAATCAAGATATGGGATGTAGCAACCGGAAAGCTACAGCTTACACTGACAGGACATACTCTTCAAATTCGAGGCCTTGCCGTTAGCAGCAAGCATCCCTATATGTTCTCTGCTGGCGATGACAAAGAAGTTAAATGTTGGGACCTTACACAGAATAAGGTCATTCGTTCATATCATGGTCATCTAAGTAGTGTTTACTGCTTGGCAACTCATCCCACTCTTGATATTCTACTCACAGGGGGACGTGATTCAGTATGTCGGGTTTGGGATATTCGTACCCAAACGCATGTTCATGCATTCTTGGGGCACAACAGCACAGTTTGCTCGGTTTTCACTAGACCAATCGATCCCCTAGTTGTCACTGGCTCGCACGATGCAACCATCAAGTTCTGGGACCTTAGAGGTGGAAAAGCAATGTCAACTCTCACAAACCATAAGAAATCCGTGCGTGCAATGGCGCCATATCCTAAGGATCAGCATTGTTTTACTTCTGCGTCAGTAGACAACATTAAGAAGTTCAACCTTCCGAGAGGGGAATTTTTACACAATATGCTAGCTCAGCAGAACACCATAGTTAACACAATGGCGGTCAATGGTGATGGAGTTATGGCCACCGGAGATGACAGTGGGAGTTTGTGGTTTTGGGATTGGGAGAGTGGCCATAACTATCAGCAATCCCAAACAACTGTACAGCCAGGTTCACTGAATAGTGAAGCTGGTATTTGTGCTCTTTCTTATGATGTCACTGGCACAAGGCTAGTCACTTGTGGAGTTGACAAGACGATCAAAATGTGGAAACAAGACGAAAACGCCACACCAGAAACTCATCCTCTCAATTTCAGTCCGCCTAAAGATATCCGGCACCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
WD40

Protein Analysis

320

Amino Acids

35.52

Weight (kDa)

8.37

Isoelectric Point (pI)

32.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 2 - 87 1.1e-08 CAF1B/HIR1 beta-propeller domain
WD40_Gbeta PF25391 3 - 141 2.5e-17 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 3 - 112 1.3e-13 CDC20/Fizzy WD40 domain
WD40 PF00400 3 - 37 4.6e-08 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 4 - 122 2.1e-25 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 4 - 100 2.9e-07 TEP-1 second beta-propeller
WD40_Prp19 PF24814 5 - 141 6.9e-23 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 5 - 258 2.3e-46 WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 7 - 80 8.3e-06 MABP1/WDR62 second WD40 domain
Beta-prop_THOC3 PF25174 10 - 103 8.5e-16 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 12 - 89 9e-11 WDHD1 first WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 14 - 84 1.7e-06 WDR90/POC16, second beta-propeller
WDR55 PF24796 14 - 168 1e-16 WDR55
Beta-prop_WDR3_2nd PF25172 18 - 121 6.5e-18 WDR3 second beta-propeller domain
Beta-prop_WDR41 PF25178 22 - 125 2e-09 WDR41
WD40 PF00400 41 - 79 6.4e-07 WD domain, G-beta repeat
EIF3I PF24805 45 - 130 3.4e-08 EIF3I
Beta-prop_TEP1_2nd PF25047 46 - 184 5.6e-15 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 48 - 171 3.7e-14 WDR36/Utp21 second beta-propeller domain
WD40_RFWD3 PF23419 50 - 141 3e-07 E3 ubiquitin-protein ligase RFWD3 WD40 domain
Beta-prop_EIPR1 PF23609 52 - 162 1e-07 EIPR1 beta-propeller
WD40_MABP1-WDR62_2nd PF24782 63 - 199 2.2e-06 MABP1/WDR62 second WD40 domain
Beta-prop_THOC3 PF25174 66 - 141 1.3e-11 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 72 - 196 1.6e-19 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 75 - 200 1.2e-12 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 83 - 204 1.8e-21 WDR3 first beta-propeller domain
WD40 PF00400 84 - 121 2.8e-08 WD domain, G-beta repeat
Beta-prop_EML PF23409 87 - 305 1.2e-17 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3 PF25174 92 - 302 4.8e-23 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 95 - 258 5.1e-12 WDHD1 first WD40 domain
WD40_Prp19 PF24814 96 - 296 2.4e-23 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 96 - 259 7.4e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 106 - 235 1e-09 WDR90/POC16, second beta-propeller
WD40_Gbeta PF25391 109 - 224 1.8e-12 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 128 - 239 6e-08 WDR3 second beta-propeller domain
EIF3I PF24805 130 - 297 1.3e-06 EIF3I
WDR55 PF24796 170 - 251 3.2e-07 WDR55
Beta-prop_WDR3_2nd PF25172 180 - 302 5.8e-07 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 194 - 297 9.2e-06 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 550, 954
AccI GTMKAC 1 cut(s) 594
AciI CCGC 2 cut(s) 680, 938
AclWI GGATC 3 cut(s) 37, 434, 573
AcoI YGGCCR 3 cut(s) 19, 702, 751
AcsI RAATTY 2 cut(s) 159, 629
AcyI GRCGYC 1 cut(s) 551
AfaI GTAC 3 cut(s) 5, 370, 783
AgsI TTSAA 2 cut(s) 158, 613
AjiI CACGTC 1 cut(s) 338
AjnI CCWGG 1 cut(s) 787
AjuI GAANNNNNNNTTGG 2 cut(s) 366, 398
AluBI AGCT 4 cut(s) 127, 133, 650, 809
AluI AGCT 4 cut(s) 127, 133, 650, 809
Alw21I GWGCWC 1 cut(s) 823
AlwI GGATC 3 cut(s) 37, 434, 573
AoxI GGCC 4 cut(s) 19, 166, 702, 751
ApeKI GCWGC 1 cut(s) 180
ApoI RAATTY 2 cut(s) 159, 629
AspLEI GCGC 1 cut(s) 553
AspS9I GGNCC 2 cut(s) 235, 487
AsuHPI GGTGA 2 cut(s) 22, 701
AsuNHI GCTAGC 1 cut(s) 646
AvaII GGWCC 2 cut(s) 235, 487
AxyI CCTNAGG 1 cut(s) 561
BaeGI GKGCMC 1 cut(s) 402
BalI TGGCCA 3 cut(s) 21, 704, 753
BanI GGYRCC 2 cut(s) 550, 954
Bbv12I GWGCWC 1 cut(s) 823
BbvI GCAGC 1 cut(s) 192
BccI CCATC 2 cut(s) 482, 686
BceAI ACGGC 1 cut(s) 158
BciT130I CCWGG 1 cut(s) 789
BfaI CTAG 4 cut(s) 429, 446, 647, 851
BfmI CTRYAG 2 cut(s) 33, 128
BfoI RGCGCY 1 cut(s) 554
BisI GCNGC 1 cut(s) 181
BlpI GCTNAGC 1 cut(s) 651
BlsI GCNGC 1 cut(s) 182
Bme1390I CCNGG 1 cut(s) 789
Bme18I GGWCC 2 cut(s) 235, 487
BmgBI CACGTC 1 cut(s) 338
BmgT120I GGNCC 2 cut(s) 235, 487
BmiI GGNNCC 4 cut(s) 236, 488, 552, 956
BmrFI CCNGG 1 cut(s) 789
BmsI GCATC 2 cut(s) 196, 457
BmtI GCTAGC 1 cut(s) 650
Bpu1102I GCTNAGC 1 cut(s) 651
Bsa29I ATCGAT 1 cut(s) 438
BsaHI GRCGYC 1 cut(s) 551
BsaWI WCCGGW 2 cut(s) 119, 707
Bse1I ACTGG 3 cut(s) 82, 460, 844
Bse21I CCTNAGG 1 cut(s) 561
Bse3DI GCAATG 2 cut(s) 513, 552
BseBI CCWGG 1 cut(s) 789
BseCI ATCGAT 1 cut(s) 438
BseGI GGATG 4 cut(s) 115, 187, 304, 919
BseMI GCAATG 2 cut(s) 513, 552
BseMII CTCAG 2 cut(s) 96, 665
BseNI ACTGG 3 cut(s) 82, 460, 844
BseSI GKGCMC 1 cut(s) 402
BseXI GCAGC 1 cut(s) 192
BshFI GGCC 4 cut(s) 21, 168, 704, 753
BshNI GGYRCC 2 cut(s) 550, 954
BshVI ATCGAT 1 cut(s) 438
BsiHKAI GWGCWC 1 cut(s) 823
BsiSI CCGG 3 cut(s) 120, 708, 952
BslFI GGGAC 3 cut(s) 248, 348, 500
BsmFI GGGAC 3 cut(s) 248, 348, 500
BsmI GAATGC 1 cut(s) 389
BsnI GGCC 4 cut(s) 21, 168, 704, 753
Bsp1286I GDGCHC 2 cut(s) 402, 823
Bsp1407I TGTACA 2 cut(s) 3, 781
Bsp143I GATC 5 cut(s) 29, 88, 439, 565, 876
Bsp1720I GCTNAGC 1 cut(s) 651
BspACI CCGC 2 cut(s) 680, 938
BspANI GGCC 4 cut(s) 21, 168, 704, 753
BspCNI CTCAG 2 cut(s) 95, 664
BspDI ATCGAT 1 cut(s) 438
BspLI GGNNCC 4 cut(s) 236, 488, 552, 956
BspOI GCTAGC 1 cut(s) 650
BspPI GGATC 3 cut(s) 37, 434, 573
BspT107I GGYRCC 2 cut(s) 550, 954
BsrDI GCAATG 2 cut(s) 513, 552
BsrGI TGTACA 2 cut(s) 3, 781
BsrI ACTGG 3 cut(s) 82, 460, 844
BssMI GATC 5 cut(s) 29, 88, 439, 565, 876
BssNI GRCGYC 1 cut(s) 551
Bst2UI CCWGG 1 cut(s) 789
Bst4CI ACNGT 3 cut(s) 412, 719, 781
Bst6I CTCTTC 1 cut(s) 159
BstACI GRCGYC 1 cut(s) 551
BstAPI GCANNNNNTGC 1 cut(s) 414
BstAUI TGTACA 2 cut(s) 3, 781
BstC8I GCNNGC 5 cut(s) 185, 208, 461, 543, 648
BstDEI CTNAG 5 cut(s) 82, 278, 491, 561, 651
BstF5I GGATG 4 cut(s) 115, 187, 304, 919
BstH2I RGCGCY 1 cut(s) 554
BstHHI GCGC 1 cut(s) 553
BstKTI GATC 5 cut(s) 32, 91, 442, 568, 879
BstMBI GATC 5 cut(s) 29, 88, 439, 565, 876
BstMWI GCNNNNNNNGC 2 cut(s) 414, 652
BstNI CCWGG 1 cut(s) 789
BstNSI RCATGY 1 cut(s) 383
BstSCI CCNGG 1 cut(s) 787
BstSFI CTRYAG 2 cut(s) 33, 128
BstSLI GKGCMC 1 cut(s) 402
BstV1I GCAGC 1 cut(s) 192
BstX2I RGATCY 1 cut(s) 29
BstYI RGATCY 1 cut(s) 29
Bsu15I ATCGAT 1 cut(s) 438
Bsu36I CCTNAGG 1 cut(s) 561
BsuRI GGCC 4 cut(s) 21, 168, 704, 753
BsuTUI ATCGAT 1 cut(s) 438
BtgZI GCGATG 1 cut(s) 225
BtrI CACGTC 1 cut(s) 338
BtsCI GGATG 4 cut(s) 115, 187, 304, 919
BtsIMutI CAGTG 6 cut(s) 75, 137, 453, 724, 794, 837
Cac8I GCNNGC 5 cut(s) 185, 208, 461, 543, 648
CfoI GCGC 1 cut(s) 553
Cfr13I GGNCC 2 cut(s) 235, 487
ClaI ATCGAT 1 cut(s) 438
CseI GACGC 1 cut(s) 576
Csp6I GTAC 3 cut(s) 4, 369, 782
CviAII CATG 3 cut(s) 269, 380, 386
CviQI GTAC 3 cut(s) 4, 369, 782
DdeI CTNAG 5 cut(s) 82, 278, 491, 561, 651
DinI GGCGCC 1 cut(s) 552
DpnI GATC 5 cut(s) 31, 90, 441, 567, 878
DpnII GATC 5 cut(s) 29, 88, 439, 565, 876
EaeI YGGCCR 3 cut(s) 19, 702, 751
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
EciI GGCGGA 1 cut(s) 927
Eco147I AGGCCT 1 cut(s) 168
Eco32I GATATC 1 cut(s) 949
Eco47I GGWCC 2 cut(s) 235, 487
Eco81I CCTNAGG 1 cut(s) 561
EcoO109I RGGNCCY 2 cut(s) 235, 487
EcoRII CCWGG 1 cut(s) 787
EcoRV GATATC 1 cut(s) 949
EcoT22I ATGCAT 1 cut(s) 391
EgeI GGCGCC 1 cut(s) 552
EheI GGCGCC 1 cut(s) 552
FaeI CATG 3 cut(s) 272, 383, 389
FaqI GGGAC 3 cut(s) 248, 348, 500
FatI CATG 3 cut(s) 268, 379, 385
FblI GTMKAC 1 cut(s) 594
Fnu4HI GCNGC 1 cut(s) 181
FokI GGATG 4 cut(s) 122, 174, 291, 906
Fsp4HI GCNGC 1 cut(s) 181
FspBI CTAG 4 cut(s) 429, 446, 647, 851
GlaI GCGC 1 cut(s) 552
GluI GCNGC 1 cut(s) 181
HaeII RGCGCY 1 cut(s) 554
HaeIII GGCC 4 cut(s) 21, 168, 704, 753
HapII CCGG 3 cut(s) 120, 708, 952
HgaI GACGC 1 cut(s) 576
HhaI GCGC 1 cut(s) 553
Hin1I GRCGYC 1 cut(s) 551
Hin1II CATG 3 cut(s) 272, 383, 389
Hin6I GCGC 1 cut(s) 551
HinP1I GCGC 1 cut(s) 551
HincII GTYRAC 3 cut(s) 513, 670, 868
HindII GTYRAC 3 cut(s) 513, 670, 868
HinfI GANTC 1 cut(s) 341
HpaI GTTAAC 1 cut(s) 670
HpaII CCGG 3 cut(s) 120, 708, 952
HphI GGTGA 2 cut(s) 22, 701
Hpy166II GTNNAC 6 cut(s) 289, 513, 595, 670, 794, 868
Hpy188I TCNGA 1 cut(s) 621
Hpy188III TCNNGA 2 cut(s) 100, 314
Hpy8I GTNNAC 6 cut(s) 289, 513, 595, 670, 794, 868
HpyAV CCTTC 1 cut(s) 626
HpyCH4III ACNGT 3 cut(s) 412, 719, 781
HpyCH4IV ACGT 1 cut(s) 337
HpyCH4V TGCA 4 cut(s) 75, 389, 470, 545
HpyF10VI GCNNNNNNNGC 2 cut(s) 414, 652
HpyF3I CTNAG 5 cut(s) 82, 278, 491, 561, 651
HpySE526I ACGT 1 cut(s) 337
Hsp92I GRCGYC 1 cut(s) 551
Hsp92II CATG 3 cut(s) 272, 383, 389
HspAI GCGC 1 cut(s) 551
KasI GGCGCC 1 cut(s) 550
KspAI GTTAAC 1 cut(s) 670
Kzo9I GATC 5 cut(s) 29, 88, 439, 565, 876
Lsp1109I GCAGC 1 cut(s) 192
LweI GCATC 2 cut(s) 196, 457
MaeI CTAG 4 cut(s) 429, 446, 647, 851
MaeII ACGT 1 cut(s) 337
MaeIII GTNAC 3 cut(s) 451, 835, 853
MalI GATC 5 cut(s) 31, 90, 441, 567, 878
MboI GATC 5 cut(s) 29, 88, 439, 565, 876
MboII GAAGA 1 cut(s) 146
MflI RGATCY 1 cut(s) 29
MhlI GDGCHC 2 cut(s) 402, 823
MlsI TGGCCA 3 cut(s) 21, 704, 753
MluCI AATT 3 cut(s) 159, 629, 928
MluNI TGGCCA 3 cut(s) 21, 704, 753
Mly113I GGCGCC 1 cut(s) 551
MnlI CCTC 4 cut(s) 158, 488, 617, 933
Mox20I TGGCCA 3 cut(s) 21, 704, 753
Mph1103I ATGCAT 1 cut(s) 391
MscI TGGCCA 3 cut(s) 21, 704, 753
MseI TTAA 3 cut(s) 225, 603, 669
MslI CAYNNNNRTG 1 cut(s) 384
Msp20I TGGCCA 3 cut(s) 21, 704, 753
MspI CCGG 3 cut(s) 120, 708, 952
MspR9I CCNGG 1 cut(s) 789
Mva1269I GAATGC 1 cut(s) 389
MvaI CCWGG 1 cut(s) 789
MwoI GCNNNNNNNGC 2 cut(s) 414, 652
NarI GGCGCC 1 cut(s) 551
NdeII GATC 5 cut(s) 29, 88, 439, 565, 876
NheI GCTAGC 1 cut(s) 646
NlaIII CATG 3 cut(s) 272, 383, 389
NlaIV GGNNCC 4 cut(s) 236, 488, 552, 956
NmuCI GTSAC 3 cut(s) 451, 835, 853
NsiI ATGCAT 1 cut(s) 391
NspI RCATGY 1 cut(s) 383
PceI AGGCCT 1 cut(s) 168
PctI GAATGC 1 cut(s) 389
PfeI GAWTC 1 cut(s) 341
PkrI GCNGC 1 cut(s) 182
PluTI GGCGCC 1 cut(s) 554
PpuMI RGGWCCY 2 cut(s) 235, 487
Psp5II RGGWCCY 2 cut(s) 235, 487
Psp6I CCWGG 1 cut(s) 787
PspGI CCWGG 1 cut(s) 787
PspN4I GGNNCC 4 cut(s) 236, 488, 552, 956
PspPI GGNCC 2 cut(s) 235, 487
PspPPI RGGWCCY 2 cut(s) 235, 487
PsuI RGATCY 1 cut(s) 29
RsaI GTAC 3 cut(s) 5, 370, 783
RsaNI GTAC 3 cut(s) 4, 369, 782
RseI CAYNNNNRTG 1 cut(s) 384
SaqAI TTAA 3 cut(s) 225, 603, 669
SatI GCNGC 1 cut(s) 181
Sau3AI GATC 5 cut(s) 29, 88, 439, 565, 876
Sau96I GGNCC 2 cut(s) 235, 487
ScrFI CCNGG 1 cut(s) 789
SduI GDGCHC 2 cut(s) 402, 823
SfaNI GCATC 2 cut(s) 196, 457
SfcI CTRYAG 2 cut(s) 33, 128
SfoI GGCGCC 1 cut(s) 552
SinI GGWCC 2 cut(s) 235, 487
SmiMI CAYNNNNRTG 1 cut(s) 384
Sse9I AATT 3 cut(s) 159, 629, 928
SseBI AGGCCT 1 cut(s) 168
SsiI CCGC 2 cut(s) 680, 938
SspDI GGCGCC 1 cut(s) 550
SspMI CTAG 4 cut(s) 429, 446, 647, 851
StuI AGGCCT 1 cut(s) 168
StyD4I CCNGG 1 cut(s) 787
TaaI ACNGT 3 cut(s) 412, 719, 781
TaiI ACGT 1 cut(s) 340
TaqI TCGA 3 cut(s) 51, 163, 438
TasI AATT 3 cut(s) 159, 629, 928
TatI WGTACW 2 cut(s) 3, 781
TfiI GAWTC 1 cut(s) 341
Tru1I TTAA 3 cut(s) 225, 603, 669
Tru9I TTAA 3 cut(s) 225, 603, 669
TscAI CASTG 6 cut(s) 82, 144, 460, 724, 801, 844
TseFI GTSAC 3 cut(s) 451, 835, 853
TseI GCWGC 1 cut(s) 180
Tsp45I GTSAC 3 cut(s) 451, 835, 853
TspDTI ATGAA 2 cut(s) 252, 374
TspGWI ACGGA 1 cut(s) 526
TspRI CASTG 6 cut(s) 82, 144, 460, 724, 801, 844
VpaK11BI GGWCC 2 cut(s) 235, 487
XapI RAATTY 2 cut(s) 159, 629
XceI RCATGY 1 cut(s) 383
XcmI CCANNNNNNNNNTGG 1 cut(s) 63
XmiI GTMKAC 1 cut(s) 594
XspI CTAG 4 cut(s) 429, 446, 647, 851
Zsp2I ATGCAT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.