MD05G1262800.v1.1

Protein pleiotropic regulatory locus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002996
Physical Location & Seq
Reverse (-)
39842632 .. 39846316
3685 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1262800.v1.1.491

Sequence Viewer

Length: 1617 bp
ATGGAGTCGTCACTTGGAAAGTTAATTTCTAAATCTCTAAAGAGACCTGGCGATTTATTTTTCCTCACCCATGGCAGCGAACTGGCCTCTCCTGACCTGCAGACTAAAAAATTTCGCATGACTTACAAGGCAAGCATGGAGTACAGTGGACTTAAACCGACGGCCCATCGGCAAAATCCCGCCGCCGACTGCGATGAATCGTCCAATGCTCTTCCCCTTGATCCAAATCCTAATACTGCTAGGAGCCCTTCAATCACCCAAGGAGCTCAAAACGCTGATTTGTTTTTGAGTTCCGCACCCAACAAGGCCAAGGCGCTGATTAATATTGCTGATGGCACTTCCGTTTTTTTTGCTCCTGGTTCGCCCCGTTCCCGCGATCAGAGGTGTTCAACAACAACGCTGGATCTAGCCCGAAATTGGCCACGTCCCATTTGGCATAGCCCGTGGAAGATTTACAGGGTCATCAGTGGCCACCTAGGATGGGTTCGCTGTGTTGCTTTTGATCCAAGCAACAGATGGTTCTGTACAGGATCTGCAGACCGGACAATCAAAATATGGGACGTAGCAAGCGGGACGCTACAACTTTCTCTTACCGGACACACTGGGCAAATACGAAGCCTTGCTCTTAGCACCAGGCAACCCTATTTGTTCTCGGCTGGCGATGACAAACAAGTCAAATGCTGGGACCTTGAACAGAACAAGGTTATCCGTTCGTATCACGGTCATCTTAGTGGCGTCTACAGCATGGCTCTCCATCTGACTCTTGATGTTTTAGTCACCGGGGGACGGGATAGCGTATGCCGTGTTTGGGATGTTCGTCTCAAGAATGCGCAAGTTCATGCATTGACTGGGCACGAAGATGCAGTTTGCTCCCTTCTCACCCTCCCAACAGATCCACAAGTTATTAGTGGGTCTCACGATACAACTATCAAGCTGTGGGACCTTAGAAAGGGAAAAACCTTTTCAACTTTAACGCATCATAAGAAATCGGTACGGGCAATGACACATCATCCTAAGGTAGATCGGGGTAATTGCTTCGCATCGGCTTCGACAAACACCATAAAAAAGTTCAACCTTCCGAGGGGTGAGTTTTTGCACAACATGCTCTCCCAACACAAGTCCATTATCAACGCGATGGCCGTCAATGAAGATGGAGTTATGGCTACAGGAGGCGACAACGGTAGCTTGTGGACCGAGTCACCATCGAAGAGAGGCTCGCAACGAACTAGGGATCCCTGCACTGCGGCTCGACGACACGGAGCAAAGCTCCGGCGAGTTTTCCCCATTTTTTTCCGTCGGGTTTTTGCTTCGGAACGGTCGTGGGTGAGCACACCCACTTTTCCGGTGAACCCGTGCACCCCGAGGACTTTCCCGGTCACCTCCGACCGAGTTACGACGTTTGGAAGTGGAGAAATTCTAAATCCTTATTCGCGCGACTCTTCTGCTAAGGAATATTTGCACCTAATCCCGGCGTGGAGACACTTCCGCAGCAGCATCTTCGGATCCTCTCGATCTGACAAGACAATCAAGATGCGGAAACAAGACGAAAAGGCCACCCGGGAAACTCATCCTCTAAATTTTAGGCCTCCTAATCTTAAAGACGTTGGGCGGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
WD40

Protein Analysis

539

Amino Acids

60.09

Weight (kDa)

9.98

Isoelectric Point (pI)

42.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR41 PF25178 147 - 273 1.3e-08 WDR41
Beta-prop_CAF1B_HIR1 PF24105 148 - 237 1e-07 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 150 - 187 1.2e-11 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 150 - 216 2e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 150 - 248 8.9e-18 THOC3 beta-propeller domain
WD40_Gbeta PF25391 150 - 292 6.4e-19 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 150 - 271 2.6e-20 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 153 - 272 2.3e-28 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 153 - 272 3.8e-12 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 155 - 334 1.2e-40 WDR5 beta-propeller domain
WD40_Prp19 PF24814 162 - 239 4.2e-14 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 162 - 238 1.3e-13 WDHD1 first WD40 domain
Beta-prop_ACSF4 PF13570 164 - 329 7.8e-06 Beta-alanine-activating enzyme, beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 164 - 234 6.4e-07 WDR90/POC16, second beta-propeller
WDR55 PF24796 165 - 320 5.8e-17 WDR55
WD40 PF00400 192 - 229 2.1e-07 WD domain, G-beta repeat
EIF3I PF24805 195 - 273 4.2e-07 EIF3I
Beta-prop_WDR36-Utp21_2nd PF25168 198 - 324 6.8e-11 WDR36/Utp21 second beta-propeller domain
WD40_RFWD3 PF23419 200 - 298 2.5e-07 E3 ubiquitin-protein ligase RFWD3 WD40 domain
WD40_CDC20-Fz PF24807 204 - 339 3.3e-22 CDC20/Fizzy WD40 domain
Beta-prop_SCAP PF24017 220 - 317 2.4e-06 SCAP Beta-propeller
WD40 PF00400 234 - 271 3.4e-06 WD domain, G-beta repeat
Beta-prop_EML PF23409 237 - 397 3.1e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 238 - 396 3.9e-07 WDR36/Utp21 first beta-propeller
Beta-prop_TEP1_2nd PF25047 240 - 335 3.6e-09 TEP-1 second beta-propeller
WD40_Prp19 PF24814 244 - 394 5.9e-18 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 246 - 386 3.8e-17 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 246 - 385 1.3e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 246 - 395 1.8e-10 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 258 - 339 8.4e-17 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 259 - 378 1.7e-11 G protein beta WD-40 repeat protein
WD40 PF00400 278 - 314 2.5e-07 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 279 - 387 1.1e-12 WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 279 - 357 1.7e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 281 - 392 2.9e-06 WDR3 second beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 671
Acc16I TGCGCA 1 cut(s) 831
Acc36I ACCTGC 1 cut(s) 105
AccI GTMKAC 1 cut(s) 738
AccII CGCG 4 cut(s) 375, 1133, 1432, 1434
AciI CCGC 9 cut(s) 180, 183, 294, 373, 570, 1244, 1486, 1534, 1609
AclWI GGATC 9 cut(s) 215, 411, 497, 538, 887, 1226, 1239, 1497, 1510
AcoI YGGCCR 3 cut(s) 419, 469, 1137
AcsI RAATTY 3 cut(s) 110, 1413, 1576
AcyI GRCGYC 1 cut(s) 735
AfaI GTAC 3 cut(s) 143, 526, 993
AfiI CCNNNNNNNGG 7 cut(s) 417, 481, 786, 808, 949, 1081, 1468
AgsI TTSAA 5 cut(s) 252, 390, 692, 966, 1072
AjiI CACGTC 1 cut(s) 425
AjnI CCWGG 3 cut(s) 46, 355, 632
AloI GAACNNNNNNTCC 2 cut(s) 1216, 1248
AluBI AGCT 4 cut(s) 266, 934, 1185, 1267
AluI AGCT 4 cut(s) 266, 934, 1185, 1267
Alw21I GWGCWC 3 cut(s) 268, 1331, 1358
Alw26I GTCTC 4 cut(s) 37, 824, 918, 1471
Alw44I GTGCAC 1 cut(s) 1354
AlwI GGATC 9 cut(s) 215, 411, 497, 538, 887, 1226, 1239, 1497, 1510
AlwNI CAGNNNCTG 1 cut(s) 533
Ama87I CYCGRG 2 cut(s) 1360, 1557
AoxI GGCC 8 cut(s) 84, 162, 306, 419, 469, 1137, 1551, 1583
ApaLI GTGCAC 1 cut(s) 1354
ApeKI GCWGC 3 cut(s) 75, 1488, 1491
ApoI RAATTY 3 cut(s) 110, 1413, 1576
AseI ATTAAT 1 cut(s) 321
AspA2I CCTAGG 1 cut(s) 475
AspLEI GCGC 3 cut(s) 316, 832, 1434
AspS9I GGNCC 4 cut(s) 163, 685, 940, 1191
AsuC2I CCSGG 5 cut(s) 781, 1373, 1469, 1558, 1559
AsuHPI GGTGA 9 cut(s) 58, 247, 769, 871, 1097, 1191, 1336, 1357, 1369
AvaI CYCGRG 2 cut(s) 1360, 1557
AvaII GGWCC 3 cut(s) 685, 940, 1191
AvrII CCTAGG 1 cut(s) 475
AxyI CCTNAGG 1 cut(s) 1014
BaeGI GKGCMC 2 cut(s) 855, 1358
BalI TGGCCA 2 cut(s) 421, 471
BamHI GGATCC 2 cut(s) 1231, 1502
BanII GRGCYC 2 cut(s) 248, 268
Bbv12I GWGCWC 3 cut(s) 268, 1331, 1358
BbvI GCAGC 3 cut(s) 87, 1500, 1503
BccI CCATC 8 cut(s) 174, 326, 474, 510, 762, 1129, 1145, 1210
BceAI ACGGC 3 cut(s) 177, 786, 1124
BcgI CGANNNNNNTGC 6 cut(s) 1029, 1063, 1424, 1458, 1480, 1514
BciT130I CCWGG 3 cut(s) 48, 357, 634
BcnI CCSGG 5 cut(s) 781, 1373, 1469, 1558, 1559
BcoDI GTCTC 4 cut(s) 37, 824, 918, 1471
BfaI CTAG 5 cut(s) 240, 407, 476, 1227, 1615
BfmI CTRYAG 4 cut(s) 98, 534, 739, 1164
BfoI RGCGCY 1 cut(s) 317
BfuAI ACCTGC 1 cut(s) 105
BisI GCNGC 5 cut(s) 76, 183, 1245, 1489, 1492
BlnI CCTAGG 1 cut(s) 475
BlsI GCNGC 5 cut(s) 77, 184, 1246, 1490, 1493
Bme1390I CCNGG 8 cut(s) 48, 357, 634, 781, 1373, 1469, 1558, 1559
Bme18I GGWCC 3 cut(s) 685, 940, 1191
BmeT110I CYCGRG 2 cut(s) 1360, 1557
BmgBI CACGTC 1 cut(s) 425
BmgT120I GGNCC 4 cut(s) 163, 685, 940, 1191
BmiI GGNNCC 5 cut(s) 245, 686, 941, 1233, 1504
BmrFI CCNGG 8 cut(s) 48, 357, 634, 781, 1373, 1469, 1558, 1559
BmrI ACTGGG 2 cut(s) 612, 858
BmsI GCATC 5 cut(s) 850, 985, 1049, 1503, 1521
BmuI ACTGGG 2 cut(s) 612, 858
BplI GAGNNNNNCTC 2 cut(s) 1251, 1283
Bpu10I CCTNAGC 1 cut(s) 1446
BpuEI CTTGAG 1 cut(s) 806
BpuMI CCSGG 5 cut(s) 781, 1373, 1469, 1558, 1559
BsaBI GATNNNNATC 1 cut(s) 225
BsaHI GRCGYC 1 cut(s) 735
BsaI GGTCTC 2 cut(s) 37, 918
BsaJI CCNNGG 9 cut(s) 70, 259, 309, 443, 475, 780, 1079, 1361, 1557
BsaWI WCCGGW 3 cut(s) 540, 593, 1342
BsaXI ACNNNNNCTCC 2 cut(s) 1091, 1121
Bsc4I CCNNNNNNNGG 7 cut(s) 417, 481, 786, 808, 949, 1081, 1468
Bse1I ACTGG 3 cut(s) 87, 607, 853
Bse21I CCTNAGG 1 cut(s) 1014
Bse3DI GCAATG 1 cut(s) 1005
Bse8I GATNNNNATC 1 cut(s) 225
BseBI CCWGG 3 cut(s) 48, 357, 634
BseDI CCNNGG 9 cut(s) 70, 259, 309, 443, 475, 780, 1079, 1361, 1557
BseGI GGATG 4 cut(s) 485, 817, 1009, 1567
BseJI GATNNNNATC 1 cut(s) 225
BseLI CCNNNNNNNGG 7 cut(s) 417, 481, 786, 808, 949, 1081, 1468
BseMI GCAATG 1 cut(s) 1005
BseNI ACTGG 3 cut(s) 87, 607, 853
BseSI GKGCMC 2 cut(s) 855, 1358
BseXI GCAGC 3 cut(s) 87, 1500, 1503
BseYI CCCAGC 1 cut(s) 681
BsgI GTGCAG 1 cut(s) 1222
Bsh1236I CGCG 4 cut(s) 375, 1133, 1432, 1434
Bsh1285I CGRYCG 2 cut(s) 1319, 1387
BshFI GGCC 8 cut(s) 86, 164, 308, 421, 471, 1139, 1553, 1585
BsiEI CGRYCG 2 cut(s) 1319, 1387
BsiHKAI GWGCWC 3 cut(s) 268, 1331, 1358
BsiHKCI CYCGRG 2 cut(s) 1360, 1557
BsiSI CCGG 8 cut(s) 541, 594, 780, 1270, 1343, 1373, 1469, 1558
BslFI GGGAC 6 cut(s) 411, 572, 586, 698, 798, 953
BslI CCNNNNNNNGG 7 cut(s) 417, 481, 786, 808, 949, 1081, 1468
BsmAI GTCTC 4 cut(s) 37, 824, 918, 1471
BsmBI CGTCTC 1 cut(s) 824
BsmFI GGGAC 6 cut(s) 411, 572, 586, 698, 798, 953
BsmI GAATGC 1 cut(s) 832
BsnI GGCC 8 cut(s) 86, 164, 308, 421, 471, 1139, 1553, 1585
Bso31I GGTCTC 2 cut(s) 37, 918
BsoBI CYCGRG 2 cut(s) 1360, 1557
Bsp1286I GDGCHC 5 cut(s) 248, 268, 855, 1331, 1358
Bsp1407I TGTACA 1 cut(s) 524
Bsp19I CCATGG 1 cut(s) 70
BspACI CCGC 9 cut(s) 180, 183, 294, 373, 570, 1244, 1486, 1534, 1609
BspANI GGCC 8 cut(s) 86, 164, 308, 421, 471, 1139, 1553, 1585
BspFNI CGCG 4 cut(s) 375, 1133, 1432, 1434
BspLI GGNNCC 5 cut(s) 245, 686, 941, 1233, 1504
BspMAI CTGCAG 2 cut(s) 102, 538
BspMI ACCTGC 1 cut(s) 105
BspPI GGATC 9 cut(s) 215, 411, 497, 538, 887, 1226, 1239, 1497, 1510
BspQI GCTCTTC 1 cut(s) 216
BspTNI GGTCTC 2 cut(s) 37, 918
BsrDI GCAATG 1 cut(s) 1005
BsrGI TGTACA 1 cut(s) 524
BsrI ACTGG 3 cut(s) 87, 607, 853
BssECI CCNNGG 9 cut(s) 70, 259, 309, 443, 475, 780, 1079, 1361, 1557
BssNI GRCGYC 1 cut(s) 735
BssT1I CCWWGG 4 cut(s) 70, 259, 309, 475
Bst2UI CCWGG 3 cut(s) 48, 357, 634
Bst4CI ACNGT 4 cut(s) 146, 722, 1181, 1317
Bst6I CTCTTC 3 cut(s) 216, 1202, 1444
BstACI GRCGYC 1 cut(s) 735
BstAPI GCANNNNNTGC 1 cut(s) 1102
BstAUI TGTACA 1 cut(s) 524
BstC8I GCNNGC 4 cut(s) 133, 568, 658, 1217
BstDEI CTNAG 5 cut(s) 626, 728, 944, 1014, 1446
BstDSI CCRYGG 2 cut(s) 70, 443
BstEII GGTNACC 1 cut(s) 1375
BstENI CCTNNNNNAGG 1 cut(s) 947
BstF5I GGATG 4 cut(s) 485, 817, 1009, 1567
BstFNI CGCG 4 cut(s) 375, 1133, 1432, 1434
BstH2I RGCGCY 1 cut(s) 317
BstHHI GCGC 3 cut(s) 316, 832, 1434
BstMAI GTCTC 4 cut(s) 37, 824, 918, 1471
BstMCI CGRYCG 2 cut(s) 1319, 1387
BstMWI GCNNNNNNNGC 3 cut(s) 272, 741, 1102
BstNI CCWGG 3 cut(s) 48, 357, 634
BstNSI RCATGY 1 cut(s) 1105
BstPI GGTNACC 1 cut(s) 1375
BstSCI CCNGG 8 cut(s) 46, 355, 632, 779, 1371, 1467, 1556, 1557
BstSFI CTRYAG 4 cut(s) 98, 534, 739, 1164
BstSLI GKGCMC 2 cut(s) 855, 1358
BstUI CGCG 4 cut(s) 375, 1133, 1432, 1434
BstV1I GCAGC 3 cut(s) 87, 1500, 1503
BstX2I RGATCY 5 cut(s) 403, 530, 892, 1231, 1502
BstYI RGATCY 5 cut(s) 403, 530, 892, 1231, 1502
Bsu36I CCTNAGG 1 cut(s) 1014
BsuRI GGCC 8 cut(s) 86, 164, 308, 421, 471, 1139, 1553, 1585
BtgI CCRYGG 2 cut(s) 70, 443
BtgZI GCGATG 3 cut(s) 207, 675, 1148
BtrI CACGTC 1 cut(s) 425
BtsCI GGATG 4 cut(s) 485, 817, 1009, 1567
BtsI GCAGTG 1 cut(s) 1239
BtsIMutI CAGTG 4 cut(s) 151, 472, 600, 1239
BveI ACCTGC 1 cut(s) 105
Cac8I GCNNGC 4 cut(s) 133, 568, 658, 1217
CaiI CAGNNNCTG 1 cut(s) 533
CfoI GCGC 3 cut(s) 316, 832, 1434
Cfr13I GGNCC 4 cut(s) 163, 685, 940, 1191
Cfr9I CCCGGG 1 cut(s) 1557
CseI GACGC 2 cut(s) 583, 724
Csp6I GTAC 3 cut(s) 142, 525, 992
CviAII CATG 6 cut(s) 71, 118, 136, 745, 839, 1102
CviQI GTAC 3 cut(s) 142, 525, 992
DdeI CTNAG 5 cut(s) 626, 728, 944, 1014, 1446
DrdI GACNNNNNNGTC 1 cut(s) 671
DseDI GACNNNNNNGTC 1 cut(s) 671
EaeI YGGCCR 3 cut(s) 419, 469, 1137
Eam1104I CTCTTC 3 cut(s) 216, 1202, 1444
EarI CTCTTC 3 cut(s) 216, 1202, 1444
Ecl136II GAGCTC 1 cut(s) 266
Eco130I CCWWGG 4 cut(s) 70, 259, 309, 475
Eco147I AGGCCT 1 cut(s) 1585
Eco24I GRGCYC 2 cut(s) 248, 268
Eco31I GGTCTC 2 cut(s) 37, 918
Eco47I GGWCC 3 cut(s) 685, 940, 1191
Eco53kI GAGCTC 1 cut(s) 266
Eco81I CCTNAGG 1 cut(s) 1014
Eco88I CYCGRG 2 cut(s) 1360, 1557
Eco91I GGTNACC 1 cut(s) 1375
EcoICRI GAGCTC 1 cut(s) 266
EcoNI CCTNNNNNAGG 1 cut(s) 947
EcoO109I RGGNCCY 2 cut(s) 685, 940
EcoO65I GGTNACC 1 cut(s) 1375
EcoRII CCWGG 3 cut(s) 46, 355, 632
EcoT14I CCWWGG 4 cut(s) 70, 259, 309, 475
EcoT22I ATGCAT 1 cut(s) 844
EcoT38I GRGCYC 2 cut(s) 248, 268
ErhI CCWWGG 4 cut(s) 70, 259, 309, 475
Esp3I CGTCTC 1 cut(s) 824
FaeI CATG 6 cut(s) 74, 121, 139, 748, 842, 1105
FaqI GGGAC 6 cut(s) 411, 572, 586, 698, 798, 953
FatI CATG 6 cut(s) 70, 117, 135, 744, 838, 1101
FauI CCCGC 3 cut(s) 187, 380, 563
FblI GTMKAC 1 cut(s) 738
Fnu4HI GCNGC 5 cut(s) 76, 183, 1245, 1489, 1492
FokI GGATG 4 cut(s) 492, 824, 996, 1554
FriOI GRGCYC 2 cut(s) 248, 268
Fsp4HI GCNGC 5 cut(s) 76, 183, 1245, 1489, 1492
FspBI CTAG 5 cut(s) 240, 407, 476, 1227, 1615
FspI TGCGCA 1 cut(s) 831
GlaI GCGC 3 cut(s) 315, 831, 1433
GluI GCNGC 5 cut(s) 76, 183, 1245, 1489, 1492
GsaI CCCAGC 1 cut(s) 685
HaeII RGCGCY 1 cut(s) 317
HaeIII GGCC 8 cut(s) 86, 164, 308, 421, 471, 1139, 1553, 1585
HapII CCGG 8 cut(s) 541, 594, 780, 1270, 1343, 1373, 1469, 1558
HgaI GACGC 2 cut(s) 583, 724
HhaI GCGC 3 cut(s) 316, 832, 1434
Hin1I GRCGYC 1 cut(s) 735
Hin1II CATG 6 cut(s) 74, 121, 139, 748, 842, 1105
Hin6I GCGC 3 cut(s) 314, 830, 1432
HinP1I GCGC 3 cut(s) 314, 830, 1432
HinfI GANTC 5 cut(s) 5, 197, 760, 1196, 1436
HpaII CCGG 8 cut(s) 541, 594, 780, 1270, 1343, 1373, 1469, 1558
HphI GGTGA 9 cut(s) 58, 247, 769, 871, 1097, 1191, 1336, 1357, 1369
Hpy166II GTNNAC 5 cut(s) 149, 739, 1191, 1348, 1356
Hpy188I TCNGA 7 cut(s) 381, 759, 1080, 1312, 1384, 1502, 1516
Hpy188III TCNNGA 6 cut(s) 92, 764, 823, 917, 1509, 1528
Hpy8I GTNNAC 5 cut(s) 149, 739, 1191, 1348, 1356
Hpy99I CGWCG 4 cut(s) 163, 1254, 1299, 1399
HpyAV CCTTC 3 cut(s) 258, 884, 1085
HpyCH4III ACNGT 4 cut(s) 146, 722, 1181, 1317
HpyCH4IV ACGT 4 cut(s) 424, 561, 1397, 1602
HpyCH4V TGCA 8 cut(s) 100, 536, 842, 863, 1096, 1239, 1356, 1459
HpyF10VI GCNNNNNNNGC 3 cut(s) 272, 741, 1102
HpyF3I CTNAG 5 cut(s) 626, 728, 944, 1014, 1446
HpySE526I ACGT 4 cut(s) 424, 561, 1397, 1602
Hsp92I GRCGYC 1 cut(s) 735
Hsp92II CATG 6 cut(s) 74, 121, 139, 748, 842, 1105
HspAI GCGC 3 cut(s) 314, 830, 1432
LguI GCTCTTC 1 cut(s) 216
LmnI GCTCC 6 cut(s) 243, 263, 358, 875, 1259, 1272
Lsp1109I GCAGC 3 cut(s) 87, 1500, 1503
LweI GCATC 5 cut(s) 850, 985, 1049, 1503, 1521
MaeI CTAG 5 cut(s) 240, 407, 476, 1227, 1615
MaeII ACGT 4 cut(s) 424, 561, 1397, 1602
MaeIII GTNAC 5 cut(s) 9, 775, 1197, 1375, 1390
MboII GAAGA 7 cut(s) 203, 460, 869, 1160, 1219, 1431, 1489
MflI RGATCY 5 cut(s) 403, 530, 892, 1231, 1502
MhlI GDGCHC 5 cut(s) 248, 268, 855, 1331, 1358
MlsI TGGCCA 2 cut(s) 421, 471
MluCI AATT 6 cut(s) 24, 110, 415, 1030, 1413, 1576
MluNI TGGCCA 2 cut(s) 421, 471
MlyI GAGTC 4 cut(s) 14, 754, 1205, 1430
MmeI TCCRAC 1 cut(s) 1407
Mox20I TGGCCA 2 cut(s) 421, 471
Mph1103I ATGCAT 1 cut(s) 844
MscI TGGCCA 2 cut(s) 421, 471
MseI TTAA 5 cut(s) 23, 153, 321, 971, 1596
MslI CAYNNNNRTG 2 cut(s) 729, 858
Msp20I TGGCCA 2 cut(s) 421, 471
MspI CCGG 8 cut(s) 541, 594, 780, 1270, 1343, 1373, 1469, 1558
MspR9I CCNGG 8 cut(s) 48, 357, 634, 781, 1373, 1469, 1558, 1559
Mva1269I GAATGC 1 cut(s) 832
MvaI CCWGG 3 cut(s) 48, 357, 634
MvnI CGCG 4 cut(s) 375, 1133, 1432, 1434
MwoI GCNNNNNNNGC 3 cut(s) 272, 741, 1102
NciI CCSGG 5 cut(s) 781, 1373, 1469, 1558, 1559
NcoI CCATGG 1 cut(s) 70
NlaIII CATG 6 cut(s) 74, 121, 139, 748, 842, 1105
NlaIV GGNNCC 5 cut(s) 245, 686, 941, 1233, 1504
NmeAIII GCCGAG 1 cut(s) 632
NmuCI GTSAC 4 cut(s) 9, 775, 1197, 1375
NsbI TGCGCA 1 cut(s) 831
NsiI ATGCAT 1 cut(s) 844
NspI RCATGY 1 cut(s) 1105
PceI AGGCCT 1 cut(s) 1585
PciSI GCTCTTC 1 cut(s) 216
PcsI WCGNNNNNNNCGW 2 cut(s) 1137, 1316
PctI GAATGC 1 cut(s) 832
PfeI GAWTC 1 cut(s) 197
PflFI GACNNNGTC 1 cut(s) 1195
PkrI GCNGC 5 cut(s) 77, 184, 1246, 1490, 1493
PleI GAGTC 4 cut(s) 13, 754, 1204, 1430
PpsI GAGTC 4 cut(s) 13, 754, 1204, 1430
PpuMI RGGWCCY 2 cut(s) 685, 940
PshBI ATTAAT 1 cut(s) 321
Psp124BI GAGCTC 1 cut(s) 268
Psp5II RGGWCCY 2 cut(s) 685, 940
Psp6I CCWGG 3 cut(s) 46, 355, 632
PspEI GGTNACC 1 cut(s) 1375
PspFI CCCAGC 1 cut(s) 681
PspGI CCWGG 3 cut(s) 46, 355, 632
PspN4I GGNNCC 5 cut(s) 245, 686, 941, 1233, 1504
PspPI GGNCC 4 cut(s) 163, 685, 940, 1191
PspPPI RGGWCCY 2 cut(s) 685, 940
PstI CTGCAG 2 cut(s) 102, 538
PstNI CAGNNNCTG 1 cut(s) 533
PsuI RGATCY 5 cut(s) 403, 530, 892, 1231, 1502
PsyI GACNNNGTC 1 cut(s) 1195
RsaI GTAC 3 cut(s) 143, 526, 993
RsaNI GTAC 3 cut(s) 142, 525, 992
RseI CAYNNNNRTG 2 cut(s) 729, 858
SacI GAGCTC 1 cut(s) 268
SapI GCTCTTC 1 cut(s) 216
SaqAI TTAA 5 cut(s) 23, 153, 321, 971, 1596
SatI GCNGC 5 cut(s) 76, 183, 1245, 1489, 1492
Sau96I GGNCC 4 cut(s) 163, 685, 940, 1191
SchI GAGTC 4 cut(s) 14, 754, 1205, 1430
ScrFI CCNGG 8 cut(s) 48, 357, 634, 781, 1373, 1469, 1558, 1559
SduI GDGCHC 5 cut(s) 248, 268, 855, 1331, 1358
SfaNI GCATC 5 cut(s) 850, 985, 1049, 1503, 1521
SfcI CTRYAG 4 cut(s) 98, 534, 739, 1164
SinI GGWCC 3 cut(s) 685, 940, 1191
SmaI CCCGGG 1 cut(s) 1559
SmiMI CAYNNNNRTG 2 cut(s) 729, 858
SmlI CTYRAG 1 cut(s) 821
SmoI CTYRAG 1 cut(s) 821
Sse9I AATT 6 cut(s) 24, 110, 415, 1030, 1413, 1576
SseBI AGGCCT 1 cut(s) 1585
SsiI CCGC 9 cut(s) 180, 183, 294, 373, 570, 1244, 1486, 1534, 1609
SspI AATATT 2 cut(s) 325, 1454
SspMI CTAG 5 cut(s) 240, 407, 476, 1227, 1615
SstI GAGCTC 1 cut(s) 268
StuI AGGCCT 1 cut(s) 1585
StyD4I CCNGG 8 cut(s) 46, 355, 632, 779, 1371, 1467, 1556, 1557
StyI CCWWGG 4 cut(s) 70, 259, 309, 475
TaaI ACNGT 4 cut(s) 146, 722, 1181, 1317
TaiI ACGT 4 cut(s) 427, 564, 1400, 1605
TaqI TCGA 4 cut(s) 1049, 1205, 1249, 1510
TaqII GACCGA 2 cut(s) 1208, 1401
TasI AATT 6 cut(s) 24, 110, 415, 1030, 1413, 1576
TatI WGTACW 2 cut(s) 141, 524
TauI GCSGC 2 cut(s) 185, 1247
TfiI GAWTC 1 cut(s) 197
Tru1I TTAA 5 cut(s) 23, 153, 321, 971, 1596
Tru9I TTAA 5 cut(s) 23, 153, 321, 971, 1596
TscAI CASTG 4 cut(s) 151, 472, 607, 1246
TseFI GTSAC 4 cut(s) 9, 775, 1197, 1375
TseI GCWGC 3 cut(s) 75, 1488, 1491
Tsp45I GTSAC 4 cut(s) 9, 775, 1197, 1375
TspDTI ATGAA 3 cut(s) 210, 827, 1161
TspGWI ACGGA 4 cut(s) 331, 698, 1272, 1283
TspMI CCCGGG 1 cut(s) 1557
TspRI CASTG 4 cut(s) 151, 472, 607, 1246
Tth111I GACNNNGTC 1 cut(s) 1195
VneI GTGCAC 1 cut(s) 1354
VpaK11BI GGWCC 3 cut(s) 685, 940, 1191
VspI ATTAAT 1 cut(s) 321
XagI CCTNNNNNAGG 1 cut(s) 947
XapI RAATTY 3 cut(s) 110, 1413, 1576
XceI RCATGY 1 cut(s) 1105
XcmI CCANNNNNNNNNTGG 2 cut(s) 429, 513
XmaI CCCGGG 1 cut(s) 1557
XmaJI CCTAGG 1 cut(s) 475
XmiI GTMKAC 1 cut(s) 738
XspI CTAG 5 cut(s) 240, 407, 476, 1227, 1615
Zsp2I ATGCAT 1 cut(s) 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.